CN119278264A - 改造启动子、表达载体、微生物、物质的生产方法、改造蓝细菌和改造启动子的制作方法 - Google Patents

改造启动子、表达载体、微生物、物质的生产方法、改造蓝细菌和改造启动子的制作方法 Download PDF

Info

Publication number
CN119278264A
CN119278264A CN202380042225.0A CN202380042225A CN119278264A CN 119278264 A CN119278264 A CN 119278264A CN 202380042225 A CN202380042225 A CN 202380042225A CN 119278264 A CN119278264 A CN 119278264A
Authority
CN
China
Prior art keywords
promoter
bases
modified
region
base sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202380042225.0A
Other languages
English (en)
Chinese (zh)
Inventor
若林万纱也
草间翔子
儿岛征司
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Panasonic Intellectual Property Management Co Ltd
Original Assignee
Panasonic Intellectual Property Management Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Panasonic Intellectual Property Management Co Ltd filed Critical Panasonic Intellectual Property Management Co Ltd
Publication of CN119278264A publication Critical patent/CN119278264A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN202380042225.0A 2022-05-31 2023-05-16 改造启动子、表达载体、微生物、物质的生产方法、改造蓝细菌和改造启动子的制作方法 Pending CN119278264A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2022088371 2022-05-31
JP2022-088371 2022-05-31
PCT/JP2023/018190 WO2023233996A1 (ja) 2022-05-31 2023-05-16 改変プロモーター、発現ベクター、微生物、物質の生産方法、改変シアノバクテリア及び改変プロモーターの作製方法

Publications (1)

Publication Number Publication Date
CN119278264A true CN119278264A (zh) 2025-01-07

Family

ID=89026577

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202380042225.0A Pending CN119278264A (zh) 2022-05-31 2023-05-16 改造启动子、表达载体、微生物、物质的生产方法、改造蓝细菌和改造启动子的制作方法

Country Status (6)

Country Link
US (1) US20250066801A1 (https=)
EP (1) EP4534667A4 (https=)
JP (1) JPWO2023233996A1 (https=)
CN (1) CN119278264A (https=)
MX (1) MX2024013351A (https=)
WO (1) WO2023233996A1 (https=)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6509185B1 (en) * 2000-01-07 2003-01-21 Genencor International, Inc. Mutant aprE promotor
JP2002085076A (ja) * 2000-09-13 2002-03-26 Mitsubishi Heavy Ind Ltd シネコシスチスｐｃｃ６７１４由来のプロモーター
CA2830864C (en) * 2002-04-22 2017-03-21 Genencor International, Inc. Methods of creating modified promoters resulting in varying levels of gene expression
JP4648038B2 (ja) 2004-03-05 2011-03-09 花王株式会社 改変プロモーター
EP3101129B1 (en) 2014-01-31 2020-10-28 Kaneka Corporation Microorganism having adjusted expression of r-specific enoyl-coa hydratase gene, and method for manufacturing polyhydroxyalkanoate copolymer using same
WO2021100640A1 (ja) * 2019-11-21 2021-05-27 パナソニックＩｐマネジメント株式会社 改変シアノバクテリア、改変シアノバクテリアの製造方法、及び、タンパク質の製造方法

Also Published As

Publication number Publication date
WO2023233996A1 (ja) 2023-12-07
EP4534667A1 (en) 2025-04-09
EP4534667A4 (en) 2025-12-24
JPWO2023233996A1 (https=) 2023-12-07
US20250066801A1 (en) 2025-02-27
MX2024013351A (es) 2024-12-06

Similar Documents

Publication Publication Date Title
JP6125493B2 (ja) 転写終結配列
Korneli et al. Getting the big beast to work—systems biotechnology of Bacillus megaterium for novel high-value proteins
US12098373B2 (en) Bacillus subtilis efficiently-induced expression system based on artificial series promoter
EP4578951A1 (en) Multi-fragmented polya polynucleotide stably existing in host cell and use thereof
Choi et al. Development of a high-copy-number plasmid via adaptive laboratory evolution of Corynebacterium glutamicum
CN116179513B (zh) 一种Cpf1蛋白及其在基因编辑中的应用
Lee Development and characterization of expression vectors for Corynebacterium glutamicum
CN108977890B (zh) 一种启动子库以及利用其在细菌中构建不同强度表达系统的方法
Mahanil et al. Simple transformation of the filamentous thermophilic cyanobacterium Leptolyngbya sp. KC45
JP2006526985A (ja) ｄｓＲＮＡを大量産生するための方法およびキット
CN119278264A (zh) 改造启动子、表达载体、微生物、物质的生产方法、改造蓝细菌和改造启动子的制作方法
Huo et al. Identifying amino acid overproducers using rare-codon-rich markers
CN108220317B (zh) 一种重组表达质粒及其制备方法、用途
JP2003245078A (ja) 放射線抵抗性細菌／大腸菌シャトルベクター
US20130244904A1 (en) Escherichia coli having a modified translational property
Zaleski et al. New cloning and expression vector derived from Escherichia coli plasmid pIGWZ12; a potential vector for a two-plasmid expression system
CN117264859B (zh) 一种原核表达系统及其制备方法和应用
CN116064521A (zh) 解淀粉芽孢杆菌来源的pH诱导型启动子及其应用
CN116064522A (zh) 解淀粉芽孢杆菌来源的温度诱导型启动子及其应用
Wang et al. Genetic engineering tools for the filamentous microalga Tribonema minus
CN114774459A (zh) 一种香蕉枯萎菌CRISPR/Cas9基因编辑载体、制备方法及应用
CN107974451A (zh) 一种应答3-脱氢莽草酸的生物传感器及其应用
Dafa’alla et al. Direct colony identification by PCR-miniprep
CN119955805B (zh) 一种与柑橘黄龙病抗性相关的Helitron转座子片段及其应用
US20230058740A1 (en) Robust Protein Expression Enabled by Dynamic Control over Host Proteases

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination