CN119101651A - 双分化或多分化类器官 - Google Patents

双分化或多分化类器官 Download PDF

Info

Publication number
CN119101651A
CN119101651A CN202411042018.8A CN202411042018A CN119101651A CN 119101651 A CN119101651 A CN 119101651A CN 202411042018 A CN202411042018 A CN 202411042018A CN 119101651 A CN119101651 A CN 119101651A
Authority
CN
China
Prior art keywords
tissue
cells
ventral
differentiation
differentiated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202411042018.8A
Other languages
English (en)
Chinese (zh)
Inventor
J·诺布里奇
J·A·巴格利
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Imba Molikul Institute Of Biotechnology
Original Assignee
Imba Molikul Institute Of Biotechnology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Imba Molikul Institute Of Biotechnology filed Critical Imba Molikul Institute Of Biotechnology
Publication of CN119101651A publication Critical patent/CN119101651A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/41—Hedgehog proteins; Cyclopamine (inhibitor)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/08—Coculture with; Conditioned medium produced by cells of the nervous system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Toxicology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN202411042018.8A 2017-04-25 2018-04-25 双分化或多分化类器官 Pending CN119101651A (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP17168051.5A EP3395942A1 (en) 2017-04-25 2017-04-25 Bi- or multi-differentiated organoid
EP17168051.5 2017-04-25
PCT/EP2018/060559 WO2018197544A1 (en) 2017-04-25 2018-04-25 Bi- or multi-differentiated organoid
CN201880027801.3A CN110573609A (zh) 2017-04-25 2018-04-25 双分化或多分化类器官

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201880027801.3A Division CN110573609A (zh) 2017-04-25 2018-04-25 双分化或多分化类器官

Publications (1)

Publication Number Publication Date
CN119101651A true CN119101651A (zh) 2024-12-10

Family

ID=58672346

Family Applications (2)

Application Number Title Priority Date Filing Date
CN202411042018.8A Pending CN119101651A (zh) 2017-04-25 2018-04-25 双分化或多分化类器官
CN201880027801.3A Pending CN110573609A (zh) 2017-04-25 2018-04-25 双分化或多分化类器官

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201880027801.3A Pending CN110573609A (zh) 2017-04-25 2018-04-25 双分化或多分化类器官

Country Status (10)

Country Link
US (1) US20210095260A1 (enExample)
EP (2) EP3395942A1 (enExample)
JP (1) JP7248590B2 (enExample)
KR (1) KR102692498B1 (enExample)
CN (2) CN119101651A (enExample)
AU (1) AU2018260183B2 (enExample)
CA (1) CA3061412A1 (enExample)
ES (1) ES3068551T3 (enExample)
SG (1) SG11201909470TA (enExample)
WO (1) WO2018197544A1 (enExample)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011140441A2 (en) 2010-05-06 2011-11-10 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
ES2860423T3 (es) 2014-05-28 2021-10-05 Childrens Hospital Med Ct Métodos y sistemas para convertir células precursoras en tejidos gástricos mediante diferenciación dirigida
CA2963704A1 (en) 2014-10-17 2016-04-21 Children's Hospital Medical Center In vivo model of human small intestine using pluripotent stem cells and methods of making and using same
CN109415685B (zh) 2016-05-05 2023-07-04 儿童医院医疗中心 用于体外制造胃底组织的方法和与其相关的组合物
SG10202104575WA (en) 2016-11-04 2021-06-29 Childrens Hospital Med Ct Liver organoid compositions and methods of making and using same
CA3045145A1 (en) 2016-12-05 2018-06-14 Children's Hospital Medical Center Colonic organoids and methods of making and using same
US12281334B2 (en) 2017-04-14 2025-04-22 Children's Hospital Medical Center Multi donor stem cell compositions and methods of making same
EP3658666A4 (en) * 2017-07-28 2021-04-28 Memorial Sloan-Kettering Cancer Center PRODUCTION OF A TOPOGRAPHICAL ORGANIZATION IN A THREE-DIMENSIONAL TISSUE CULTURE
WO2019074793A1 (en) 2017-10-10 2019-04-18 Children's Hospital Medical Center OESOPHAGIAN TISSUE COMPOSITIONS AND / OR ORGANOIDS AND METHODS OF MAKING SAME
JP7702784B2 (ja) * 2017-12-04 2025-07-04 ザ アドミニストレイターズ オブ ザ テューレイン エデュケイショナル ファンド スフェロイドを用いた細胞システムならびにそれらの製造方法及び使用方法
US12379372B2 (en) 2017-12-21 2025-08-05 Children's Hospital Medical Center Digitalized human organoids and methods of using same
CA3106634A1 (en) * 2018-07-26 2020-01-30 Children's Hospital Medical Center Hepato-biliary-pancreatic tissues and methods of making same
CN112823012A (zh) 2018-09-12 2021-05-18 儿童医院医学中心 用于产生造血干细胞及其衍生物的类器官组合物
WO2020160371A1 (en) 2019-02-01 2020-08-06 The University Of Hong Kong Innervated organoid compositions and methods of making same
US12534709B2 (en) 2019-05-31 2026-01-27 Children's Hospital Medical Center Shaped organoid compositions and methods of making same
CA3141807A1 (en) 2019-05-31 2020-12-03 Children's Hospital Medical Center Methods of generating and expanding hematopoietic stem cells
US20230287357A1 (en) * 2020-07-06 2023-09-14 President And Fellows Of Harvard College Programmable organoids and methods of producing the same via orthogonal differentiation and bioprinting
WO2022154080A1 (ja) * 2021-01-15 2022-07-21 国立大学法人東京大学 複雑な活動と可塑性を示すインビトロ神経回路組織、その神経活動の観察装置およびこれを用いた物質のスクリーニング方法
DE102021132190A1 (de) * 2021-12-07 2023-06-22 Eberhard Karls Universität Tübingen, Körperschaft des öffentlichen Rechts Biokompatible Strukturen zur Verbindung und Kultivierung von biologischem Material
HUP2200189A2 (hu) * 2022-05-30 2023-12-28 Biotalentum Tudasfejlesztoe Kft Új, riportert stabilan expresszáló humán indukált pluripotens õssejt
EP4286513A1 (en) 2022-06-03 2023-12-06 IMBA-Institut für Molekulare Biotechnologie GmbH Triple tissue culture fusion
CN116286342B (zh) * 2022-12-23 2025-10-28 山东大学 一种类人脑皮质芯片、3d打印类人脑皮质的方法及应用
CN116129193B (zh) * 2023-02-27 2024-06-11 重庆大学附属肿瘤医院 一种类器官生长预测方法、系统及设备
CN118530833B (zh) * 2024-04-02 2024-11-29 武汉大学 一种同步共分化的多组织或多类器官模型及其构建方法

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4829000A (en) 1985-08-30 1989-05-09 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Reconstituted basement membrane complex with biological activity
DE10003521A1 (de) 2000-01-27 2001-08-09 Medigene Ag Vorrichtung zum Herstellen eines dreidimensionalen Matrixkörpers, Multi-Well-Platte, Lösung zum Kultivieren von Säugerkardiomyocyten, Verfahren zum Kultivieren einer Zellkultur, Vorrichtung für die Messung isometrischer Kraftparameter von Zellkulturen sowie Verfahren zum meßbaren Verfolgen von Kontraktionen eines in eine Trägersubstanz eingelagerten Zellgewebes
US8021876B2 (en) 2001-11-15 2011-09-20 Children's Medical Center Corporation Methods of isolation, expansion and differentiation of fetal stem cells from chorionic villus, amniotic fluid, and placenta and therapeutic uses thereof
WO2004084950A2 (en) 2003-03-24 2004-10-07 Case Western Reserve University Cell targeting methods and compositions
US20100196923A1 (en) 2007-08-14 2010-08-05 Anthony Atala Pluripotent adult stem cells
EP2028268A1 (en) * 2007-08-20 2009-02-25 Université Libre De Bruxelles Generation of neuronal cells from pluripotent stem cells
US20110143433A1 (en) 2008-03-17 2011-06-16 Agency For Science, Technology And Research Microcarriers for Stem Cell Culture
US10793830B2 (en) * 2009-11-05 2020-10-06 Riken Method for producing retinal tissue in vitro
US20130149287A1 (en) * 2010-07-30 2013-06-13 Cambridge Enterprise Limited Corticogenesis of Human Pluripotent Cells
EP2893000B1 (en) 2012-09-03 2019-04-10 Novo Nordisk A/S Generation of pancreatic endoderm from pluripotent stem cells using small molecules
EP2743345A1 (en) * 2012-12-13 2014-06-18 IMBA-Institut für Molekulare Biotechnologie GmbH Three dimensional heterogeneously differentiated tissue culture
KR101381798B1 (ko) * 2013-02-27 2014-04-07 씨제이제일제당 (주) 신규 박테리오파지 및 이를 포함하는 항균 조성물
US9677085B2 (en) * 2013-03-18 2017-06-13 Massachusetts Institute Of Technology Engineering a heterogeneous tissue from pluripotent stem cells
JP6756610B2 (ja) * 2013-04-26 2020-09-16 メモリアル スローン ケタリング キャンサー センター 多分化能細胞および多能性細胞の分化を方向付けることによって発生させる皮質介在ニューロンおよびその他のニューロン細胞
US10794802B2 (en) * 2013-09-20 2020-10-06 California Institute Of Technology Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high resolution intact circuit mapping and phenotyping
US11767507B2 (en) * 2013-11-08 2023-09-26 The Mclean Hospital Corporation Methods for efficient generation of GABAergic interneurons from pluripotent stem cells
KR102317610B1 (ko) 2013-11-22 2021-10-26 고쿠리쓰 겐큐 가이하쓰 호징 리가가쿠 겐큐소 종뇌 또는 그 선구 조직의 제조 방법
US10081792B2 (en) * 2014-12-31 2018-09-25 Wisconsin Alumni Research Foundation Derivation of human microglia from pluripotent stem cells
AU2016226178B2 (en) * 2015-03-03 2021-07-29 President And Fellows Of Harvard College Methods of generating functional human tissue
US10087417B2 (en) * 2015-04-22 2018-10-02 William J. Freed Three-dimensional model of human cortex
US10081791B2 (en) * 2015-05-07 2018-09-25 William J. Freed Modeling connections between dopaminergic neurons and the cerebral cortex
LU92845B1 (en) * 2015-10-08 2017-05-02 Univ Du Luxembourg Campus Belval Means and methods for generating midbrain organoids
US10597623B2 (en) * 2015-11-13 2020-03-24 The Johns Hopkins University Multiwell cell culture system having rotating shafts for mixing culture media and method of use thereof
WO2017117547A1 (en) * 2015-12-31 2017-07-06 President And Fellows Of Harvard College Methods for generating neural tissue and uses thereof
SG11201807487WA (en) * 2016-03-14 2018-09-27 Agency Science Tech & Res Generation of midbrain-specific organoids from human pluripotent stem cells
FR3058892B1 (fr) * 2016-11-23 2021-04-09 Univ Bordeaux Unite de tissu neural et utilisation d'une telle unite pour l'implantation dans le systeme nerveux d'un mammifere
US10676715B2 (en) * 2017-03-28 2020-06-09 The Board Of Trustees Of The Leland Stanford Junior University Assembly of functionally integrated human forebrain spheroids and methods of use thereof
KR102695118B1 (ko) * 2017-03-29 2024-08-16 이스트만 케미칼 컴파니 위치-선택적으로 치환된 셀룰로스 에스터
GB201900930D0 (en) * 2019-01-23 2019-03-13 Res & Innovation Uk Choroid plexus organoids and methods for productions thereof
WO2020247336A1 (en) * 2019-06-03 2020-12-10 The Regents Of The University Of California Pifithrin analogues and methods of treating rett syndrome

Also Published As

Publication number Publication date
EP3615661B1 (en) 2026-02-11
AU2018260183A1 (en) 2019-10-31
EP3615661A1 (en) 2020-03-04
AU2018260183B2 (en) 2024-09-19
CN110573609A (zh) 2019-12-13
WO2018197544A1 (en) 2018-11-01
US20210095260A1 (en) 2021-04-01
ES3068551T3 (en) 2026-06-01
JP2020517283A (ja) 2020-06-18
CA3061412A1 (en) 2018-11-01
EP3395942A1 (en) 2018-10-31
SG11201909470TA (en) 2019-11-28
JP7248590B2 (ja) 2023-03-29
KR102692498B1 (ko) 2024-08-05
EP3615661C0 (en) 2026-02-11
KR20190141231A (ko) 2019-12-23

Similar Documents

Publication Publication Date Title
EP3615661B1 (en) Bi- or multi-differentiated organoid
ES2987290T3 (es) Modelo de tejido cardiaco
KR102656200B1 (ko) 혈관 오가노이드, 상기 오가노이드의 제조 및 사용 방법
EP3072960B1 (en) Method for manufacturing telencephalon or progenitor tissue thereof
CN110494555B (zh) 具有分支结构的肺芽类器官的生成和其用于肺疾病建模的用途
EP2679673B1 (en) Method of producing retinal pigment epithelial cell sheet
Ribatti et al. Epithelial-mesenchymal interactions: a fundamental developmental biology mechanism
Peretz et al. A new role of hindbrain boundaries as pools of neural stem/progenitor cells regulated by Sox2
Hocevar et al. Matrigel is required for efficient differentiation of isolated, stem cell-derived otic vesicles into inner ear organoids
KR20170110579A (ko) 신장 오가노이드를 형성시키기 위한 다능성 줄기세포의 분화
Völkner et al. Mouse retinal organoid growth and maintenance in longer-term culture
KR20180025817A (ko) 탈세포화된 세포외 기질을 포함하는 직접교차분화 촉진용 조성물 및 이의 용도
AU2004241380A1 (en) Preparation of endodermal stem cells
Tang‐Schomer et al. In vitro 3D regeneration‐like growth of human patient brain tissue
EP3630948A1 (en) Methods for chemically induced lineage reprogramming
Malheiro et al. 3D culture platform of human iPSCs-derived nociceptors for peripheral nerve modeling and tissue innervation
Olmsted et al. Fully characterized mature human iPS-and NMP-derived motor neurons thrive without neuroprotection in the spinal contusion cavity
Eintracht et al. Efficient embryoid-based method to improve generation of optic vesicles from human induced pluripotent stem cells
KR20220146467A (ko) 연골세포를 사용하여 연골 이식체를 제공하는 방법
HK40014216A (en) Bi- or multi-differentiated organoid
US20260049290A1 (en) Human spinal cord injury organoids
US20240287430A1 (en) Systems and Methods for Cellular Lumen Formation and Cellular Differentiation
Pinto Engineering Vascularized Organoids in a Defined Fibrin-based Hydrogel
Hosseini et al. Combined Effect of Decellularized Rat Brain Tissue and Nerve Growth Factor on 5 Neural Differentiation of PC12 Cell Line in 2D Cell Culture
Schickel Development of human brain tumor assembloids that mimic the interaction of normal and tumorous tissue to assess the impact of ionizing radiation

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination