CN118984871A - 制备人多能干细胞衍生的睾丸间质样细胞和人多能干细胞衍生的睾丸间质样细胞群的方法 - Google Patents

制备人多能干细胞衍生的睾丸间质样细胞和人多能干细胞衍生的睾丸间质样细胞群的方法 Download PDF

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CN118984871A
CN118984871A CN202380032988.7A CN202380032988A CN118984871A CN 118984871 A CN118984871 A CN 118984871A CN 202380032988 A CN202380032988 A CN 202380032988A CN 118984871 A CN118984871 A CN 118984871A
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cells
pluripotent stem
cell
testosterone
derived
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Chinese (zh)
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青井贵之
佐藤克哉
藤泽正人
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Kobe University NUC
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Kobe University NUC
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    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/52—Sperm; Prostate; Seminal fluid; Leydig cells of testes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/10—Drugs for genital or sexual disorders; Contraceptives for impotence
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0683—Cells of the male genital tract, e.g. prostate, epididymis; Non-germinal cells from testis, e.g. Leydig cells, Sertoli cells
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/165—Vascular endothelial growth factor [VEGF]
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2510/00—Genetically modified cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Reproductive Health (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
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  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
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  • Wood Science & Technology (AREA)
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  • Animal Behavior & Ethology (AREA)
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  • Endocrinology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Gynecology & Obstetrics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202380032988.7A 2022-02-15 2023-02-08 制备人多能干细胞衍生的睾丸间质样细胞和人多能干细胞衍生的睾丸间质样细胞群的方法 Pending CN118984871A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2022021533 2022-02-15
JP2022-021533 2022-02-15
PCT/JP2023/004081 WO2023157727A1 (ja) 2022-02-15 2023-02-08 ヒト多能性幹細胞由来ライディッヒ様細胞の作製方法及びヒト多能性幹細胞由来ライディッヒ様細胞集団

Publications (1)

Publication Number Publication Date
CN118984871A true CN118984871A (zh) 2024-11-19

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CN202380032988.7A Pending CN118984871A (zh) 2022-02-15 2023-02-08 制备人多能干细胞衍生的睾丸间质样细胞和人多能干细胞衍生的睾丸间质样细胞群的方法

Country Status (5)

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US (1) US20250186507A1 (https=)
EP (1) EP4481036A4 (https=)
JP (1) JPWO2023157727A1 (https=)
CN (1) CN118984871A (https=)
WO (1) WO2023157727A1 (https=)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2026049146A (ja) * 2024-09-06 2026-03-18 国立大学法人神戸大学 ヒト多能性幹細胞由来ライディッヒ様細胞の作製方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8048999B2 (en) 2005-12-13 2011-11-01 Kyoto University Nuclear reprogramming factor
EP2596096B1 (en) * 2010-07-21 2018-01-31 Kyoto University Method for inducing differentiation of human pluripotent stem cell into intermediate mesoderm cell
FR3014316B1 (fr) * 2013-12-10 2017-01-20 Defymed Organe bioartificiel
JP6531335B2 (ja) * 2015-07-17 2019-06-19 国立大学法人京都大学 血管内皮細胞の誘導方法
WO2018088240A1 (ja) 2016-11-10 2018-05-17 国立大学法人神戸大学 ヒト多能性幹細胞由来ライディッヒ様細胞の作製方法及びヒト多能性幹細胞由来ライディッヒ様細胞
JP6831147B1 (ja) 2020-07-22 2021-02-17 アサヒエンジニアリング株式会社 横抗の形成方法及び横抗

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US20250186507A1 (en) 2025-06-12
EP4481036A4 (en) 2026-03-11
JPWO2023157727A1 (https=) 2023-08-24
EP4481036A1 (en) 2024-12-25
WO2023157727A1 (ja) 2023-08-24

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