CN118974243A - 哺乳动物细胞冷冻保存液 - Google Patents

哺乳动物细胞冷冻保存液 Download PDF

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Publication number
CN118974243A
CN118974243A CN202380032758.0A CN202380032758A CN118974243A CN 118974243 A CN118974243 A CN 118974243A CN 202380032758 A CN202380032758 A CN 202380032758A CN 118974243 A CN118974243 A CN 118974243A
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CN
China
Prior art keywords
cell
dextran
cells
trehalose
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202380032758.0A
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English (en)
Chinese (zh)
Inventor
西村益浩
小森奈月
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Otsuka Pharmaceutical Factory Inc
Original Assignee
Otsuka Pharmaceutical Factory Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Otsuka Pharmaceutical Factory Inc filed Critical Otsuka Pharmaceutical Factory Inc
Publication of CN118974243A publication Critical patent/CN118974243A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/56—Physical preservation processes for animal cells or human cells
    • C12N5/562—Temperature processes, e.g. following predefined temperature changes over time
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/52—Chemical aspects of preservation of animal cells or human cells
    • C12N5/522—Preservation media
    • C12N5/525—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202380032758.0A 2022-04-12 2023-03-03 哺乳动物细胞冷冻保存液 Pending CN118974243A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2022065637 2022-04-12
JP2022-065637 2022-04-12
PCT/JP2023/008060 WO2023199641A1 (ja) 2022-04-12 2023-03-03 哺乳動物細胞凍結保存液

Publications (1)

Publication Number Publication Date
CN118974243A true CN118974243A (zh) 2024-11-15

Family

ID=88329352

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202380032758.0A Pending CN118974243A (zh) 2022-04-12 2023-03-03 哺乳动物细胞冷冻保存液

Country Status (5)

Country Link
US (1) US20250263673A1 (https=)
EP (1) EP4509596A4 (https=)
JP (1) JPWO2023199641A1 (https=)
CN (1) CN118974243A (https=)
WO (1) WO2023199641A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW202543677A (zh) * 2024-02-28 2025-11-16 美商安斯泰來再生醫藥協會 細胞冷凍保存調配物及其使用方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0646840A (ja) 1992-08-04 1994-02-22 Nippon Zenyaku Kogyo Kk 細胞凍結保存液
JPH07255469A (ja) 1994-03-17 1995-10-09 Kurabo Ind Ltd 動物細胞の凍結保存液
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
JPH08325101A (ja) 1995-05-31 1996-12-10 Seitai Kagaku Kenkyusho:Kk 動物組織保存方法
US6280718B1 (en) 1999-11-08 2001-08-28 Wisconsin Alumni Reasearch Foundation Hematopoietic differentiation of human pluripotent embryonic stem cells
JP4385158B2 (ja) 2000-12-04 2009-12-16 株式会社リンフォテック 細胞の保存液および該保存液を用いた細胞の保存方法
WO2002101057A1 (fr) 2001-06-08 2002-12-19 Dnavec Research Inc. Transfert genique dans des cellules souches embryonnaires de primate a l'aide d'un virus de l'immunodeficience simienne de pseudo type vsv-g utilise comme vecteur
CA2474968A1 (en) 2002-01-31 2003-08-07 Asahi Techno Glass Corporation Cryopreservation medium for primate embryo stem cells and cryopreservation method
JP5867912B2 (ja) * 2011-05-11 2016-02-24 国立大学法人福井大学 細胞の凍結保存液および凍結保存方法
WO2019172112A1 (ja) 2018-03-06 2019-09-12 ゼノアックリソース株式会社 細胞凍結保存用溶液およびその利用
JP7524077B2 (ja) * 2019-01-17 2024-07-29 長瀬産業株式会社 細胞凍結保存液
JP2021000027A (ja) 2019-06-21 2021-01-07 帝人株式会社 医療用細胞凍結保存液

Also Published As

Publication number Publication date
EP4509596A1 (en) 2025-02-19
US20250263673A1 (en) 2025-08-21
JPWO2023199641A1 (https=) 2023-10-19
WO2023199641A1 (ja) 2023-10-19
EP4509596A4 (en) 2026-04-01

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