CN118272451A - Method for preparing struvite by recycling nitrogen and phosphorus in sewage - Google Patents
Method for preparing struvite by recycling nitrogen and phosphorus in sewage Download PDFInfo
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- CN118272451A CN118272451A CN202410384199.6A CN202410384199A CN118272451A CN 118272451 A CN118272451 A CN 118272451A CN 202410384199 A CN202410384199 A CN 202410384199A CN 118272451 A CN118272451 A CN 118272451A
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- struvite
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- rhodococcus erythropolis
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title claims abstract description 152
- 229910052567 struvite Inorganic materials 0.000 title claims abstract description 100
- CKMXBZGNNVIXHC-UHFFFAOYSA-L ammonium magnesium phosphate hexahydrate Chemical compound [NH4+].O.O.O.O.O.O.[Mg+2].[O-]P([O-])([O-])=O CKMXBZGNNVIXHC-UHFFFAOYSA-L 0.000 title claims abstract description 93
- 229910052757 nitrogen Inorganic materials 0.000 title claims abstract description 77
- 229910052698 phosphorus Inorganic materials 0.000 title claims abstract description 68
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 title claims abstract description 67
- 239000011574 phosphorus Substances 0.000 title claims abstract description 67
- 239000010865 sewage Substances 0.000 title claims abstract description 56
- 238000000034 method Methods 0.000 title claims abstract description 44
- 238000004064 recycling Methods 0.000 title claims abstract description 31
- 241000187561 Rhodococcus erythropolis Species 0.000 claims abstract description 60
- 230000001580 bacterial effect Effects 0.000 claims abstract description 36
- 239000000725 suspension Substances 0.000 claims abstract description 29
- 238000004519 manufacturing process Methods 0.000 claims abstract description 18
- 230000003213 activating effect Effects 0.000 claims abstract description 5
- 239000002351 wastewater Substances 0.000 claims description 29
- 230000008859 change Effects 0.000 claims description 18
- 239000001963 growth medium Substances 0.000 claims description 17
- JOPOVCBBYLSVDA-UHFFFAOYSA-N chromium(6+) Chemical compound [Cr+6] JOPOVCBBYLSVDA-UHFFFAOYSA-N 0.000 claims description 9
- 239000002504 physiological saline solution Substances 0.000 claims description 9
- 239000012137 tryptone Substances 0.000 claims description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 229940041514 candida albicans extract Drugs 0.000 claims description 8
- 239000012138 yeast extract Substances 0.000 claims description 8
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 6
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- BHEPBYXIRTUNPN-UHFFFAOYSA-N hydridophosphorus(.) (triplet) Chemical compound [PH] BHEPBYXIRTUNPN-UHFFFAOYSA-N 0.000 claims 3
- 238000002425 crystallisation Methods 0.000 abstract description 7
- 230000008025 crystallization Effects 0.000 abstract description 7
- 239000011651 chromium Substances 0.000 description 23
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 16
- 230000033558 biomineral tissue development Effects 0.000 description 15
- 229910001385 heavy metal Inorganic materials 0.000 description 14
- 239000013078 crystal Substances 0.000 description 11
- 239000002609 medium Substances 0.000 description 9
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 8
- 229910052804 chromium Inorganic materials 0.000 description 8
- KMUONIBRACKNSN-UHFFFAOYSA-N potassium dichromate Chemical compound [K+].[K+].[O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O KMUONIBRACKNSN-UHFFFAOYSA-N 0.000 description 8
- 238000011084 recovery Methods 0.000 description 8
- MXZRMHIULZDAKC-UHFFFAOYSA-L ammonium magnesium phosphate Chemical compound [NH4+].[Mg+2].[O-]P([O-])([O-])=O MXZRMHIULZDAKC-UHFFFAOYSA-L 0.000 description 7
- 241000863430 Shewanella Species 0.000 description 6
- 238000001035 drying Methods 0.000 description 6
- 238000004062 sedimentation Methods 0.000 description 6
- 238000000926 separation method Methods 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
- 238000012512 characterization method Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 235000019441 ethanol Nutrition 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 210000003608 fece Anatomy 0.000 description 5
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- 241000894006 Bacteria Species 0.000 description 4
- 239000003513 alkali Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000003344 environmental pollutant Substances 0.000 description 4
- 230000008014 freezing Effects 0.000 description 4
- 238000007710 freezing Methods 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 231100000719 pollutant Toxicity 0.000 description 4
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 3
- 229910052749 magnesium Inorganic materials 0.000 description 3
- 239000011777 magnesium Substances 0.000 description 3
- 239000003208 petroleum Substances 0.000 description 3
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 2
- 238000005033 Fourier transform infrared spectroscopy Methods 0.000 description 2
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
- 241000316848 Rhodococcus <scale insect> Species 0.000 description 2
- 238000002441 X-ray diffraction Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- TXCDCPKCNAJMEE-UHFFFAOYSA-N dibenzofuran Chemical compound C1=CC=C2C3=CC=CC=C3OC2=C1 TXCDCPKCNAJMEE-UHFFFAOYSA-N 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
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- 239000003337 fertilizer Substances 0.000 description 2
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
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- 230000001737 promoting effect Effects 0.000 description 2
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- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- NLHHRLWOUZZQLW-UHFFFAOYSA-N Acrylonitrile Chemical compound C=CC#N NLHHRLWOUZZQLW-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 229910017958 MgNH Inorganic materials 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- YUWBVKYVJWNVLE-UHFFFAOYSA-N [N].[P] Chemical compound [N].[P] YUWBVKYVJWNVLE-UHFFFAOYSA-N 0.000 description 1
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 150000001491 aromatic compounds Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000009388 chemical precipitation Methods 0.000 description 1
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- 230000003247 decreasing effect Effects 0.000 description 1
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- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- IINNWAYUJNWZRM-UHFFFAOYSA-L erythrosin B Chemical compound [Na+].[Na+].[O-]C(=O)C1=CC=CC=C1C1=C2C=C(I)C(=O)C(I)=C2OC2=C(I)C([O-])=C(I)C=C21 IINNWAYUJNWZRM-UHFFFAOYSA-L 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 125000001741 organic sulfur group Chemical group 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
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- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a method for preparing struvite by recycling nitrogen and phosphorus in sewage, which comprises the following steps: s1, domesticating rhodococcus erythropolis to obtain domesticated rhodococcus erythropolis; s2, activating the domesticated rhodococcus erythropolis to obtain bacterial suspension; the bacterial density in the bacterial suspension is OD 600 =0.400-0.800; and S3, inoculating the bacterial suspension into nitrogen and phosphorus containing sewage to be treated for culture to obtain struvite. The rhodococcus erythropolis used in the invention can produce struvite in sewage, and the domesticated rhodococcus erythropolis can improve the alkalinity of the culture solution, and can improve the alkalinity of sewage when used in sewage containing nitrogen and phosphorus, promote the crystallization of struvite, thereby obviously increasing the production amount of struvite. The method is simple and effective, has lower production cost and wider application range.
Description
Technical Field
The invention relates to the technical field of sewage treatment, in particular to a method for preparing struvite by recycling nitrogen and phosphorus in sewage.
Background
Along with the continuous improvement of the requirements of various emission standards on sewage nutrient substances, the requirement of resource recycling is urgent, the aim of resource recovery through a water treatment technology becomes a great research hot spot in the sewage treatment field, and a struvite crystallization recovery process is developed.
Struvite, also known as magnesium ammonium phosphate (Magnesium Ammonium Phosphate, MAP), has the formula: (MgNH 4PO 4. 6H2O), a white crystal which is difficult to dissolve in water. The magnesium ammonium phosphate is colorless and odorless, is a non-silt crystal, is rich in nutrient elements required by the growth of crops such as N (nitrogen), P (phosphorus), magnesium and the like, is not easy to dissolve in water, can slowly release N, P, magnesium and other elements, and is a very ideal slow-release fertilizer. Compared with the method of adding magnesium source, alkali source and the like, the method for producing magnesium ammonium phosphate by microorganism induced mineralization can save recovery cost and reduce the scale formation risk of a sewage treatment system. Publication CN 112852886A proposes mineralization recovery of phosphorus in wastewater by using shiva bacteria. However, the mineralization effect in the prior art is easily influenced by water quality and the content of pollutants in the water quality, the production amount of struvite is small, and under the condition of high concentration of heavy metal, the content of heavy metal in mineralized products is high, so that potential safety hazards in the struvite recycling process are easily caused.
Disclosure of Invention
The invention aims to solve the technical problems of low struvite production and high heavy metal content in mineralized products, and provides a method for preparing struvite by recycling nitrogen and phosphorus in sewage.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a method for preparing struvite by recycling nitrogen and phosphorus in sewage comprises the following steps:
S1, domesticating rhodococcus erythropolis to obtain domesticated rhodococcus erythropolis;
S2, activating the domesticated rhodococcus erythropolis to obtain bacterial suspension; the bacterial density in the bacterial suspension is OD 600 =0.400-0.800;
And S3, inoculating the bacterial suspension into nitrogen and phosphorus containing sewage to be treated for culture to obtain struvite.
In some embodiments, step S1 specifically includes: inoculating the rhodococcus erythropolis into a nitrogen source culture medium for domestication, wherein the culture medium contains 2-8g/L of tryptone and 1-4g/L of yeast extract.
In some embodiments, the acclimation is performed at a temperature of 25-40deg.C and a rotational speed of 120-200r/min for a period of 3-10d.
In some embodiments, in step S1, pH change is measured, and acclimation is stopped when the pH in the medium reaches above 8.5, to obtain acclimated rhodococcus erythropolis.
In some embodiments, step S2 specifically includes: inoculating the domesticated rhodococcus erythropolis into a culture medium for facultative anaerobic culture, and then mixing with physiological saline to prepare bacterial suspension.
In some embodiments, the medium is LB medium, the temperature of the facultative anaerobic culture is 25-35 ℃, the rotation speed is 120-200r/min, and the time is 16-24h.
In some embodiments, in step S3, the culture is a facultative anaerobic culture at a temperature of 25-30℃and a rotational speed of 120-200 r/min for a period of 48-144 hours.
In some embodiments, the tryptone content in the nitrogen and phosphorus containing wastewater is 5-10g/L, the yeast extract content is 2-5g/L, the sodium chloride content is 5-10g/L, the magnesium chloride content is 0.399-0.410 g/L, and the dipotassium hydrogen phosphate content is 0.408-0.452 g/L.
In some embodiments, the hexavalent chromium content in the nitrogen and phosphorus containing wastewater is between 0 and 0.8mmol/L.
In some embodiments, the hexavalent chromium content in the nitrogen and phosphorus containing wastewater is between 0 and 0.2mmol/L.
Compared with the prior art, the invention has the beneficial effects that:
According to the method for preparing struvite by recycling nitrogen and phosphorus in sewage, provided by the invention, nitrogen and phosphorus in sewage are removed by utilizing rhodococcus erythropolis, struvite is recycled, and the domesticated rhodococcus erythropolis has the capability of improving the alkalinity of a culture solution, so that the method has a good effect in removing nitrogen and phosphorus in sewage and recycling struvite, and the production amount of struvite is obviously increased and is superior to that of the existing Shewanella. The method is simple and effective, has lower production cost and wider application range.
In some embodiments, the ability of rhodococcus erythropolis to decompose nitrogen sources in a nitrogen source medium is domesticated, so that the metabolism is promoted, ammonium generated by decomposition is increased, and thus the alkali production ability of rhodococcus erythropolis is improved, and the domestication is performed under mineralization conditions instead of pollutant conditions, so that the mineralization ability of rhodococcus erythropolis is improved, and the phosphorus recovery ability is enhanced.
In some embodiments, the method can reduce the occurrence of chromium in struvite in the presence of hexavalent chromium, resulting in a more safe struvite.
Compared with the prior art, the method has the remarkable advantages that:
Firstly, the domesticated rhodococcus erythropolis has the capability of improving the alkalinity of the culture solution, and the pH value of the environment can be effectively regulated in the process of removing nitrogen and phosphorus in sewage, so that the formation of struvite is facilitated, the production amount of struvite is increased, and the recovery efficiency is improved. Secondly, the invention improves the mineralization capability, promotes the metabolism of rhodococcus erythropolis by domesticating the capability of decomposing nitrogen sources in the nitrogen source culture medium, so that the ammonium generated by the decomposition becomes more, thereby not only improving the alkali production capability of rhodococcus erythropolis, but also enhancing the recovery capability of rhodococcus erythropolis to phosphorus. In addition, the invention can reduce the occurrence amount of chromium in struvite under the condition that heavy metal hexavalent chromium exists. Therefore, the present invention can safely recover nitrogen and phosphorus even in sewage containing heavy metals and produce struvite with higher safety. This is of great practical importance for the treatment of industrial waste water containing heavy metals. In summary, the method for recycling nitrogen and phosphorus in sewage and preparing struvite by using rhodococcus erythropolis has the advantages of improving struvite production, enhancing mineralization capacity, reducing heavy metal content in struvite and the like, and is a simple, effective, low-production-cost and wide-application-range solution.
Other advantages of embodiments of the present invention are further described below.
Drawings
FIG. 1 is a flow chart of a method for preparing struvite by recycling nitrogen and phosphorus in sewage according to an embodiment of the invention;
FIGS. 2a to 2b are SEM photographs of a recovered product of struvite crystallization in example 1 of the present invention;
FIG. 3 is an XRD pattern of a recovered product of struvite crystallization in example 1 of the present invention;
FIG. 4 is a FT-IR image of the recovered product of struvite crystallization in example 1 of the invention;
FIG. 5 is a graph showing the total nitrogen variation in examples 1 to 4 of the present invention;
fig. 6 is a graph showing the total phosphorus variation in examples 1 to 4 of the present invention.
Detailed Description
The application will be further described with reference to the following drawings in conjunction with the preferred embodiments. It should be noted that, without conflict, the embodiments of the present application and features of the embodiments may be combined with each other.
Chromium (vi) reduces the respiratory activity of bacteria in the sludge and affects the performance of the activated sludge system, resulting in a decrease in its removal of COD (Chemical Oxygen Demand ) and nitrate nitrogen. The magnesium ammonium phosphate mineralization can realize nitrogen and phosphorus removal in the wastewater, has the advantages of low cost, no secondary pollution and the like, so that the magnesium ammonium phosphate crystallization is generated by utilizing the chromium (VI) resistant microorganism mineralization, and the removal of organic matters and heavy metals in the high-salt wastewater has great economic benefit and environmental protection value. Rhodococcus erythropolis is a gram positive bacterium belonging to the genus Rhodococcus. The main uses of known rhodococcus strains include: the method is used for producing lactic acid by fermentation in the food industry, producing enzymes and metabolites such as erythrosin, amino acid and the like in the field of biological medicine, and catalyzing acrylonitrile hydration to produce acrylamide in petroleum exploitation, and is mainly used for petroleum degradation, degrading phenol in industrial wastewater, organic sulfur-containing aromatic compounds in petroleum, toluene, indole, dibenzofuran and other organic matters, and being applied to the industrial production of acrylic acid and acrylamide, biological desulfurization and the like in water treatment.
The invention provides a method for preparing struvite by utilizing nitrogen and phosphorus in rhodococcus erythropolis recovered sewage, which improves the alkali production capacity of rhodococcus erythropolis by domesticating the capacity of decomposing nitrogen sources in a nitrogen source culture medium, and applies the rhodococcus erythropolis to prepare struvite by recycling nitrogen and phosphorus in the sewage. The invention utilizes rhodococcus erythropolis to recycle nitrogen and phosphorus in sewage to produce struvite, the capability is better than that of the prior Shewanella, the heavy metal content in the produced struvite can be reduced under the existence of heavy metal Cr (VI), the struvite with higher safety is produced, the addition of chemical reagents is avoided, the safe recycling of products is realized, and a better technological solution is provided for nitrogen and phosphorus treatment of sewage and mineralized phosphorus recycling of microorganisms.
As shown in fig. 1, the method for preparing struvite by recycling nitrogen and phosphorus in sewage provided by the embodiment of the invention comprises the following steps:
S1, domesticating rhodococcus erythropolis to obtain domesticated rhodococcus erythropolis. The method specifically comprises the following steps:
(1) Rhodococcus erythropolis (Rhodococcus erythropolis, NP-11) is selected.
(2) Inoculating Rhodococcus erythropolis stored in a freezing tube into 100ml of culture medium containing nitrogen source as main component (tryptone content of 2-8g/L tryptone and yeast extract content of 1-4 g/L), domesticating at 25-40deg.C and rotation speed of 120-200r/min for 3-10d, and measuring pH of fermentation broth.
(3) After the domestication is completed, the rhodococcus erythropolis is transferred to an inclined plane and stored in a freezing tube. Preferably, the acclimation is stopped when the pH in the medium reaches above 8.5.
S2, activating the domesticated rhodococcus erythropolis to obtain bacterial suspension. The method specifically comprises the following steps:
Inoculating the strain domesticated and preserved in the step S1 into a conical flask filled with 100ml LB culture medium, activating for 16-24h at 25-35 ℃ and rotating speed of 120-200r/min, and mixing with physiological saline to prepare bacterial suspension. The bacterial density in the bacterial suspension is OD 600 =0.400-0.800.
S3, inoculating the bacterial suspension in an equal volume into the sewage containing nitrogen and phosphorus to be treated for culture, wherein the nitrogen content is 2870mg/L and the phosphorus content is 220mg/L. The method specifically comprises the following steps:
Inoculating the bacterial suspension into the sewage containing nitrogen and phosphorus to be treated, and performing facultative anaerobic culture for 48-144h under the conditions that the temperature is 25-30 ℃ and the rotating speed is 120-200 r/min.
S4, carrying out sedimentation separation on the product after the culture is finished, washing with absolute ethyl alcohol, and drying at constant temperature to obtain struvite.
In some embodiments, the tryptone content in the nitrogen-phosphorus-containing wastewater is 5-10g/L, the yeast extract content is 2-5g/L, the sodium chloride content is 5-10g/L, the magnesium chloride content is 0.399-0.410 g/L, and the dipotassium hydrogen phosphate content is 0.408-0.452 g/L.
In some embodiments, the hexavalent chromium content in the nitrogen and phosphorus containing wastewater is between 0 and 0.8mmol/L.
In some embodiments, the concentration of physiological saline is 0.85%.
The method for preparing struvite by recycling nitrogen and phosphorus in sewage is described in detail below with reference to specific examples.
Rhodococcus erythropolis domestication
In the following examples, the domestication method of rhodococcus erythropolis for removing nitrogen and phosphorus from sewage and recovering struvite comprises the following steps:
(1) The culture medium for acclimation was prepared as follows: 5g/L tryptone, 2.5g/L yeast extract.
(2) Inoculating Rhodococcus erythropolis stored in a freezing tube into a culture medium, culturing at 30deg.C and rotation speed of 120r/min, and measuring pH change to improve the decomposition capacity of the Rhodococcus erythropolis to nitrogen source.
(3) The pH value in the culture medium reaches above 8.5, the domestication is stopped, and the bacteria are transferred and stored in an inclined plane and a freezing tube.
Example 1
A method for preparing struvite by recycling nitrogen and phosphorus in sewage by using rhodococcus erythropolis comprises the following steps:
(1) The simulated sewage used in this example was prepared: 10g/L tryptone, 5g/L yeast extract, 5g/L sodium chloride, 0.399g/L MgCl 2、0.408g/L K2HPO4, autoclaved at 121℃for 20min.
(2) The rhodococcus erythropolis preserved and domesticated in example 1 was inoculated into a conical flask containing 100mL of LB medium, followed by facultative anaerobic culture at 30℃and a rotational speed of 120r/min for 24 hours, and mixed with 0.85% physiological saline to prepare a bacterial suspension. The bacterial density in the bacterial suspension is OD 600 =0.400-0.800.
(3) And inoculating the bacterial suspension into simulated sewage in an equal volume, performing facultative anaerobic culture for 144 hours at the temperature of 30 ℃ and the rotating speed of 120r/min, observing the generation condition of crystals every 24 hours, and measuring the change of the nitrogen and phosphorus content (used for reflecting the consumption condition of nitrogen and phosphorus in the system in the process of generating magnesium ammonium phosphate) and the struvite yield.
(4) After the cultivation is finished, carrying out sedimentation separation and ethanol washing on the product, and drying the product in an oven at constant temperature for 30min until the weight is constant, thus obtaining the bird droppings Dan Chanwu.
Characterization analysis was performed on the results of three parallel experiments, resulting in an average struvite yield of 499.12mg/L.
Example 2
A method for preparing struvite by recycling nitrogen and phosphorus in sewage by using rhodococcus erythropolis comprises the following steps:
(1) The simulated wastewater of example 1 was sterilized after adjusting the Cr 6+ concentration to 0.2mmol/L using potassium dichromate, to obtain the simulated wastewater of example 2.
(2) The rhodococcus erythropolis preserved and domesticated in example 1 was inoculated into a conical flask containing 100mL of LB medium, followed by facultative anaerobic culture at 30℃and a rotational speed of 120r/min for 24 hours, and mixed with 0.85% physiological saline to prepare a bacterial suspension. The bacterial density in the bacterial suspension is OD 600 =0.400-0.800.
(3) Then inoculating the bacterial suspension with equal volume into the simulated sewage of the example 3, carrying out facultative anaerobic culture for 144 hours at the temperature of 30 ℃ and the rotating speed of 120r/min, observing the generation condition of crystals every 24 hours, and measuring the change of the nitrogen and phosphorus content and the struvite yield.
(4) After the cultivation is finished, carrying out sedimentation separation and ethanol washing on the product, and drying the product in an oven at constant temperature for 30min until the weight is constant, thus obtaining the bird droppings Dan Chanwu.
Characterization analysis was performed on the results of three parallel experiments, resulting in an average struvite yield of 528.42mg/L.
Example 3
A method for preparing struvite by recycling nitrogen and phosphorus in sewage by using rhodococcus erythropolis comprises the following steps:
(1) The simulated wastewater of example 1 was sterilized after adjusting Cr 6+ content to 0.4mmol/L using potassium dichromate, to obtain the simulated wastewater of example 3.
(2) The rhodococcus erythropolis preserved and domesticated in example 1 was inoculated into a conical flask containing 100mL of LB medium, followed by facultative anaerobic culture at 30℃and a rotational speed of 120r/min for 24 hours, and mixed with 0.85% physiological saline to prepare a bacterial suspension. The bacterial density in the bacterial suspension is OD 600 =0.400-0.800.
(3) Then inoculating the bacterial suspension with equal volume into the simulated sewage in the example 4, carrying out facultative anaerobic culture for 144 hours at the temperature of 30 ℃ and the rotating speed of 120r/min, observing the generation condition of crystals every 24 hours, and measuring the change of the nitrogen and phosphorus content and the struvite yield.
(4) After the cultivation is finished, carrying out sedimentation separation and ethanol washing on the product, and drying the product in an oven at constant temperature for 30min until the weight is constant, thus obtaining the bird droppings Dan Chanwu.
Characterization analysis was performed on the results of three parallel experiments, resulting in an average struvite yield of 420.97mg/L.
Example 4
A method for preparing struvite by recycling nitrogen and phosphorus in sewage by using rhodococcus erythropolis comprises the following steps:
(1) The simulated wastewater of example 1 was sterilized after adjusting Cr 6+ content to 0.8mmol/L using potassium dichromate, to obtain the simulated wastewater of example 4.
(2) The rhodococcus erythropolis preserved and domesticated in example 1 was inoculated into a conical flask containing 100mL of LB medium, followed by facultative anaerobic culture at 30℃and a rotational speed of 120r/min for 24 hours, and mixed with 0.85% physiological saline to prepare a bacterial suspension.
(3) Then inoculating the bacterial suspension with equal volume into the simulated sewage in the example 5, carrying out facultative anaerobic culture for 144 hours at the temperature of 30 ℃ and the rotating speed of 120r/min, observing the generation condition of crystals every 24 hours, and measuring the change of the nitrogen and phosphorus content and the struvite yield.
(4) After the cultivation is finished, carrying out sedimentation separation and ethanol washing on the product, and drying the product in an oven at constant temperature for 30min until the weight is constant, thus obtaining the bird droppings Dan Chanwu.
Characterization analysis was performed on the results of three parallel experiments, resulting in an average struvite yield of 130.74mg/L.
Comparative example
A method for preparing struvite by recycling nitrogen and phosphorus in sewage by using rhodococcus erythropolis comprises the following steps:
(1) The simulated wastewater of example 2 was sterilized after adjusting Cr 6+ content to 0.8mmol/L using potassium dichromate, to obtain the simulated wastewater of the comparative example.
(2) The non-domesticated rhodococcus erythropolis is inoculated into an conical flask filled with 100mL of LB culture medium, then subjected to facultative anaerobic culture for 24 hours at the temperature of 30 ℃ and the rotating speed of 120r/min, and mixed with physiological saline with the concentration of 0.85% to prepare bacterial suspension.
(3) Then inoculating the bacterial suspension with equal volume into the simulated sewage of the control example, carrying out facultative anaerobic culture for 144 hours at the temperature of 30 ℃ and the rotating speed of 120r/min, observing the generation condition of crystals every 24 hours, and measuring the change of the nitrogen and phosphorus content and the struvite yield.
(4) After the cultivation is finished, carrying out sedimentation separation and ethanol washing on the product, and drying the product in an oven at constant temperature for 30min until the weight is constant, thus obtaining the bird droppings Dan Chanwu.
Characterization analysis was performed on the results of three parallel experiments, resulting in an average struvite yield of 80.74mg/L.
TABLE 1 Material composition and struvite yield for inventive and comparative examples
SEM pictures of the recovered products of the struvite crystals in example 1 of the present invention are shown in FIGS. 2a to 2b, XRD patterns of the recovered products of the struvite crystals in example 1 are shown in FIG. 3, and FT-IR pictures of the recovered products of the struvite crystals in example 1 are shown in FIG. 4; the total nitrogen change curves in examples 1 to 4 are shown in FIG. 5, wherein the curve corresponding to 0.0mM is the total nitrogen change curve of example 1, the curve corresponding to 0.2mM is the total nitrogen change curve of example 2, the curve corresponding to 0.5mM is the total nitrogen change curve of example 3, and the curve corresponding to 0.8mM is the total nitrogen change curve of example 4; the total phosphorus change curves in examples 1 to 4 are shown in FIG. 6, wherein the curve corresponding to 0.0mM is the total phosphorus change curve of example 1, the curve corresponding to 0.2mM is the total phosphorus change curve of example 2, the curve corresponding to 0.5mM is the total phosphorus change curve of example 3, and the curve corresponding to 0.8mM is the total phosphorus change curve of example 4.
As can be seen from table 1, fig. 2a to 2b and fig. 3 to 6, the domesticated rhodococcus erythropolis has better microorganism mineralization effect under the condition of heavy metal adjustment; when the chromium concentration is 0.2mmol/L, the struvite produced by the mineralization of rhodococcus erythropolis has more production than struvite produced under the condition without chromium, namely the concentration can promote the production of struvite; when the chromium concentration is 0.8mmol/L, rhodococcus erythropolis can still grow and induce mineralization to produce struvite; the Cr occurrence amount in the struvite of the example 2 and the example 3 is lower than 270mg/L, is lower than that in the struvite produced by the Shewanella mineralization under the same and equal conditions in the chromium-containing wastewater (the data is from Chinese patent literature with patent number 2021100429784), and accords with the national slow release fertilizer standard. The results show that the rhodococcus erythropolis has wider application range in the simulated wastewater treatment, a simpler and effective treatment method is provided for chromium-containing wastewater treatment, and the prepared struvite has higher safety and provides a new way for recycling struvite.
Unlike available technology, the present invention utilizes rhodococcus erythropolis to eliminate nitrogen and phosphorus from waste water and to recover struvite, and the domesticated rhodococcus erythropolis has raised culture liquid alkalinity and excellent effect in eliminating nitrogen and phosphorus from waste water and recovering struvite. When the simulated wastewater without Cr (VI) was recovered (example 1), struvite yield was 499.12mg/L, better than 480mg/L of Shewanella; when the Cr (VI) concentration in the sewage was 0.2mmol/L (example 2), the struvite production continued to increase, and less chromium was detected in the crystals; surprisingly, it was found that when the concentration of Cr (VI) in the wastewater was increased to 0.2mmol/L, the struvite production increased significantly as compared to when Cr (VI) was absent, and when the concentration of Cr (VI) in the wastewater was continued to be increased (examples 3-4), the struvite production decreased significantly, so that the embodiment of the invention was best for struvite conversion when applied to wastewater having a Cr (VI) content of 0-0.2mmol/L, and the struvite conversion was highest when the Cr (VI) content was 0.2 mmol/L. In addition, when the concentration of Cr (VI) in sewage is 0.8mmol/L, the capacity of the strain for improving the alkalinity of the culture solution is not obviously affected, and struvite can be produced by induced mineralization, so that the strain is proved to have feasibility in treating simulated wastewater with different concentrations. Compared with the mineralized recovery product of Shewanella, the product of the embodiment of the invention has lower Cr occurrence content and stronger practical applicability. The traditional chemical precipitation technology and the technology effect of removing nitrogen and phosphorus by using Shewanella mineralization to recycle struvite are easy to be influenced by heavy metals, and when the concentration of pollutants is high, the concentration of the pollutants in struvite is increased, thus preventing the safe utilization of struvite. Therefore, the method disclosed by the invention is favorable for promoting the combination of the sewage heavy metal treatment process and the struvite crystallization process, saving the heavy metal sewage treatment cost and promoting the application of rhodococcus erythropolis in sewage treatment.
The foregoing is a further detailed description of the invention in connection with the preferred embodiments, and it is not intended that the invention be limited to the specific embodiments described. It will be apparent to those skilled in the art that several equivalent substitutions and obvious modifications can be made without departing from the spirit of the invention, and the same should be considered to be within the scope of the invention.
Claims (10)
1. A method for preparing struvite by recycling nitrogen and phosphorus in sewage is characterized by comprising the following steps:
S1, domesticating rhodococcus erythropolis to obtain domesticated rhodococcus erythropolis;
S2, activating the domesticated rhodococcus erythropolis to obtain bacterial suspension; the bacterial density in the bacterial suspension is OD 600 =0.400-0.800;
And S3, inoculating the bacterial suspension into nitrogen and phosphorus containing sewage to be treated for culture to obtain struvite.
2. The method for preparing struvite by recycling nitrogen and phosphorus in sewage according to claim 1, wherein step S1 specifically comprises: inoculating the rhodococcus erythropolis into a nitrogen source culture medium for domestication, wherein the culture medium contains 2-8g/L of tryptone and 1-4g/L of yeast extract.
3. The method for preparing struvite by recycling nitrogen and phosphorus in sewage according to claim 2, wherein the domestication temperature is 25-40 ℃, the rotating speed of the cultivation equipment is 120-200r/min, and the domestication time is 3-10d.
4. The method for producing struvite by recycling nitrogen and phosphorus from wastewater according to claim 3, wherein in step S1, pH change is measured, and domestication is stopped when pH in a culture medium reaches 8.5 or more, thereby obtaining domesticated rhodococcus erythropolis.
5. The method for preparing struvite by recycling nitrogen and phosphorous in sewage according to any one of claims 1 to 4, wherein step S2 comprises: inoculating the domesticated rhodococcus erythropolis into a culture medium for facultative anaerobic culture, and then mixing with physiological saline to prepare bacterial suspension.
6. The method for preparing struvite by recycling nitrogen and phosphorus in sewage according to claim 5, wherein the culture medium is LB culture medium, the temperature of the facultative anaerobic culture is 25-35 ℃, the rotating speed is 120-200r/min, and the time is 16-24h.
7. The method for preparing struvite by recycling nitrogen and phosphorus from sewage according to any of claims 1 to 6, wherein in step S3, the culture is a facultative anaerobic culture at a temperature of 25-30 ℃ and a rotational speed of 120-200r/min for 48-144 hours.
8. The method for preparing struvite from recycled wastewater containing nitrogen and phosphorus according to any one of claims 1 to 7, wherein the content of tryptone in the wastewater containing nitrogen and phosphorus is 5-10g/L, the content of yeast extract is 2-5g/L, the content of sodium chloride is 5-10g/L, the content of magnesium chloride is 0.399-0.410g/L, and the content of dipotassium hydrogen phosphate is 0.408-0.452g/L.
9. The method for preparing struvite from recycled wastewater containing nitrogen and phosphorous according to any one of claims 1 to 8, wherein the hexavalent chromium content in the wastewater containing nitrogen and phosphorous is 0 to 0.8mmol/L.
10. The method for preparing struvite by recycling nitrogen and phosphorus in sewage according to claim 9, wherein the hexavalent chromium content in the nitrogen and phosphorus sewage is 0-0.2 mmol/L.
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