CN118238242A

CN118238242A - Dry lawn coloring agent and application thereof

Info

Publication number: CN118238242A
Application number: CN202410286959.XA
Authority: CN
Inventors: 李明; 龚梦华; 马伟杰; 陈浩旭; 郑先福
Original assignee: ZHENGZHOU ZHENGSHI CHEMICAL PRODUCT CO Ltd
Current assignee: ZHENGZHOU ZHENGSHI CHEMICAL PRODUCT CO Ltd
Priority date: 2024-03-13
Filing date: 2024-03-13
Publication date: 2024-06-25

Abstract

The invention relates to a dry lawn coloring agent and application thereof, belonging to the technical field of lawn greening agents. The dry lawn coloring agent consists of water, a wetting agent, an auxiliary agent and the following components in percentage by mass: 0.2 to 0.3 percent of trinexapac-ethyl, 0.2 to 0.25 percent of osthole, 20 to 25 percent of ethanol, 30 to 35 percent of water-based color paste and 25 to 30 percent of water-based acrylic emulsion. According to the invention, the green period of the lawn can be effectively prolonged by physically coloring the lawn leaves and combining with regulating the content of chlorophyll in the lawn, and spraying the lawn in the dormancy period under the combined action of the two aspects. The dry lawn coloring agent provided by the invention has the advantages of quick response, lasting coloring time and good weather resistance, can improve the safe overwintering performance of the lawn, is simple to use and is safe and harmless to the lawn.

Description

Dry lawn coloring agent and application thereof

Technical Field

The invention relates to a dry lawn coloring agent and application thereof, belonging to the technical field of lawn greening agents.

Background

In recent years, with the enhancement of environmental protection consciousness of people, urban greening areas are increased year by year, urban landscaping is fast in development, and lawns are important components of urban greening, so that the urban landscaping is popular with people because the urban landscaping can beautify the environment and provide leisure and entertainment places. Therefore, the lawn area is increased year by year, and the lawn green land becomes an important component of urban landscaping. However, due to weather and other reasons, the lawn loses green and turns yellow and gradually withers in winter, even if cool-season type lawn grass with strong cold resistance and long green period is selected to plant the lawn, the dry-yellow period of 2 to 3 months also exists in winter, so that the attractive appearance and the use value of the lawn are greatly influenced, and the prolonging of the green period of the lawn becomes one of the problems to be solved in the current production and use of the lawn.

At present, common measures for prolonging the green period of the lawn are as follows: (1) reasonable water and fertilizer management: the green period can be prolonged by 20-50d, but the lawn still enters the dormancy period; (2) electrothermal and hydrothermal heating: the heating pipeline is paved, so that the lawn can be protected from withering yellow in winter, but the cost is high, and the lawn is not easy to control; and (3) mulching: the green-turning of the lawn can be realized in advance, but the lawn still enters a dormancy period in winter and the appearance is influenced; (4) spraying a plant growth regulator: gibberellin and the like are sprayed, so that the lawn can be turned green in advance, but the lawn still enters a dormancy period in winter; (5) spraying a greening agent: the green period can reach 60-100d, but the safe overwintering performance of the lawn cannot be ensured. Among them, spraying a lawn greening agent is one of the most effective means for extending the green period of a lawn.

The Chinese patent publication No. CN102653489B, publication No. 2014, no. 10 and No. 15 discloses a lawn coloring agent, which comprises the following components, by weight, 100 parts of deionized water, 0.005-0.01 part of monopotassium phosphate, 0.002-0.005 part of potassium nitrate, 0.01-0.02 part of magnesium sulfate, 0.005-0.01 part of ferrous sulfate, 0.02-0.05 part of sodium naphthalene acetate, 0.005-0.01 part of sodium copper chlorophyllin, 0.01-0.02 part of barium stearate and 0.01-0.02 part of calcium stearate;

The Chinese patent publication No. CN102653490B, publication No. 2015, no. 07 and No. 08 disclose a lawn greening agent, which comprises the components and the content of the components of 0.03-0.05 part of potassium dihydrogen phosphate, 0.03-0.05 part of potassium nitrate, 0.05-0.1 part of sodium nitrate, 0.01-0.03 part of EDTA-Fe, 0.05-0.1 part of EDTA-Mg, 0.02-0.05 part of sodium naphthalene acetate and 0.01-0.05 part of manganese sulfate in 100 parts of deionized water;

The invention patent of China with publication number CN105399500A and publication number of 2016-03/16 discloses a nutritional lawn greening agent, and a preparation method and a use method thereof, wherein the formula comprises, by weight, 100 parts of deionized water, 0.005-0.1 part of dopamine, 0.003-0.005 part of ammonium phytate, 0.002-0.006 part of potassium sulfate, 0.008-0.015 part of amino acid iron, 0.01-0.03 part of magnesium chloride, 0.02-0.05 part of allantoin and 0.005-0.01 part of green plant dye;

The Chinese patent of the invention with publication date of 2017, 04 month 05 and application publication number of CN106554231A discloses a lawn greening agent with good adhesiveness and a preparation method thereof, and specifically discloses the lawn greening agent which is prepared from the following raw materials in parts by weight: 2-3 parts of yellow dextrin, 20-30 parts of corn gluten meal, 1-2 parts of saponin, 6-11 parts of fatty acid, 3-5 parts of monosodium glutamate waste liquid, 10-20 parts of rhamnus dulcis, a proper amount of acetic acid, a proper amount of sodium carbonate, 1-2 parts of potassium sulfate, 4-7 parts of dopamine, 2-3 parts of EDTA-iron, 5-9 parts of urea, 2-3 parts of magnesium chloride and 40-60 parts of water;

The Chinese patent of the invention with publication number CN106631373A, publication number 2017, month 05 and 10 discloses a lawn greening agent which is used for enhancing photosynthesis and contains corn gluten meal and a preparation method thereof, and specifically discloses the lawn greening agent which is prepared from the following raw materials in parts by weight: 2-3 parts of potassium nitrate, 20-30 parts of corn gluten meal, 2-4 parts of actinomycete colony, 5-10 parts of fatty acid, 3-5 parts of monosodium glutamate waste liquid, 15-25 parts of rhamnus dulcis, a proper amount of acetic acid, a proper amount of sodium carbonate, 1-3 parts of triacontanol, 4-7 parts of dopamine, 2-3 parts of EDTA-iron, 5-9 parts of urea, 2-3 parts of magnesium chloride and 40-60 parts of water; the lawn coloring agent or the lawn greening agent in the Chinese patent can prolong the green period of the lawn, but in northern areas, the winter temperature is lower, the low temperature time is as long as 2-3 months, and the stress resistance of the lawn is poor due to the low temperature, so that the lawn is easy to be affected by bacteria in winter, the green turning of the lawn in the coming year is affected, and the lawn coloring agent or the lawn greening agent has the problems of short green period, rain wash resistance, easy fading and very little effect on improving the safe overwintering performance of the lawn when being used on the lawn in northern winter.

Disclosure of Invention

The invention aims to provide a dry lawn coloring agent, which solves the problems of short green period and poor safety overwintering performance of a prolonged lawn when the lawn coloring agent or the lawn greening agent in the prior art is used on a lawn in a dormant period.

The second purpose of the invention is to provide the application of the dry lawn coloring agent in prolonging the green period of the lawn in winter, so as to solve the problems of shorter green period and poorer safe overwintering performance of the lawn coloring agent or the lawn greening agent in the prior art when the lawn coloring agent or the lawn greening agent is used on the lawn in the dormant period.

In order to achieve the above purpose, the technical scheme of the dry lawn coloring agent in the invention is as follows:

The dry lawn coloring agent consists of water, a wetting agent, an auxiliary agent and the following components in percentage by mass: 0.2 to 0.3 percent of trinexapac-ethyl, 0.2 to 0.25 percent of osthole, 20 to 25 percent of ethanol, 30 to 35 percent of water-based color paste and 25 to 30 percent of water-based acrylic emulsion.

The beneficial effects of the technical scheme are that: the dry lawn coloring agent is a pioneering invention. According to the invention, the plant growth regulator trinexapac-ethyl is selected to improve the chlorophyll content in the lawn leaves and enhance the cold resistance of the lawn under low-temperature stress; selecting a plant source bactericide osthole (7-methoxy-8-isopentenyl coumarin) to reduce the infection probability of the lawn in the coming year; simultaneously, the types and the amounts of the solvent, the water-based color paste, the film forming agent and the thickening agent are optimized, and the physical coloring and film forming technology is applied to quickly color the lawn and resist rain wash. According to the invention, the green period of the lawn can be effectively prolonged by spraying the lawn in the dormancy period under the combined action of the two aspects of physically coloring the lawn leaves and regulating the content of chlorophyll in the lawn. The dry lawn coloring agent provided by the invention has the advantages of quick response, lasting coloring time, good weather resistance, capability of improving the safe overwintering performance of the lawn, simple use and safety and harmlessness to the lawn.

Specifically, ethanol is selected as the solvent, so that the trinexapac-ethyl and osthole (7-methoxy-8-isopentenyl coumarin) can be well dissolved, are uniformly dispersed, and have good compatibility with other components; the film forming agent is water-based acrylic emulsion, so that the coloring agent can be attached to the surface of the hay to form a film; the wetting agent can enable the coloring agent to wet the surface of the hay rapidly, is beneficial to rapid film formation of the coloring agent on the surface of the hay, and reduces fading. The components are mutually matched, so that the stable performance and good water resistance of the coloring agent are ensured, and meanwhile, the film can be quickly formed on the surface of the dried grass, and the fading is further prevented.

As a further improvement, the wetting agent is octyl phenol polyoxyethylene ether; the mass percentage of the wetting agent in the coloring agent is 0.5-1%.

As a further improvement, the auxiliary agent includes a thickener; the mass percentage of the thickener in the coloring agent is 0.2-0.3%.

The beneficial effects of the technical scheme are that: the thickener can effectively increase the viscosity of the coloring agent and enhance the physical stability of the medicament.

As a further improvement, the thickener is hydroxyethyl cellulose.

As a further improvement, the auxiliary agent comprises an antifreezing agent; the mass percentage of the antifreezing agent in the coloring agent is 1-5%.

The beneficial effects of the technical scheme are that: the addition of the antifreezing agent can improve the stability of the coloring agent at low temperature.

As a further improvement, the antifreezing agent is one of ethylene glycol and 1, 2-propylene glycol.

As a further improvement, the auxiliary agent comprises one or two of an antifoaming agent and a preservative; the mass percentage of the defoaming agent in the coloring agent is 0.3-0.5%; the mass percentage of the preservative in the coloring agent is 0.1-0.3%.

The beneficial effects of the technical scheme are that: the defoaming agent can effectively eliminate foam generated in the processing and using processes of the coloring agent, and prevent the problem of uneven coloring caused by foam on the surface of the dried grass; the preservative can effectively prevent the stain from deteriorating, and the quality assurance period is prolonged.

Specifically, the formulation composition of the dry lawn stain of the present invention is shown in table 1.

TABLE 1 formulation composition of the dry lawn stain of the present invention

Further specifically, the preparation method of the dry lawn coloring agent comprises the following steps:

(1) Uniformly mixing an antifreezing agent and water, slowly adding a thickening agent under stirring, and fully dissolving for standby;

(2) The plant growth regulator, the plant source bactericide, the wetting agent and the solvent are accurately weighed according to the calculated dosage, added in sequence and stirred uniformly for standby;

(3) Respectively adding the water-based color paste, the film forming agent, the preservative and the defoaming agent, and uniformly stirring for standby;

(4) Adding the liquid in the step (1) into the liquid in the step (3) under stirring, uniformly stirring, adding the liquid in the step (2), continuously stirring, and uniformly mixing.

The preparation method of the dry lawn coloring agent is simple to operate, common chemical materials are physically mixed to form the dry lawn coloring agent, the process stability is good, the preparation process is simple, and the dry lawn coloring agent is suitable for industrial production. And the materials selected are common chemical materials, so that the market can conveniently purchase.

In order to achieve the above purpose, the technical scheme of the application of the dry lawn coloring agent in prolonging the green period of the lawn in winter is as follows:

an application of a dry lawn coloring agent in prolonging green period of lawn in winter.

The beneficial effects of the technical scheme are that: the application of the dry lawn coloring agent in prolonging the green period of the lawn is a pioneering invention. The practical application effect proves that the dry lawn coloring agent provided by the invention has the advantages of quick response, lasting coloring time, good weather resistance, capability of improving the safe overwintering performance of the lawn, simplicity in use and no harm to the safety of the lawn, and can effectively solve the problem that the green period of the lawn is prolonged when the lawn coloring agent or the lawn greening agent in the prior art is used on the lawn in the dormancy period.

As a further improvement, the diluted dye is sprayed on the lawn in the dormant period.

The beneficial effects of the technical scheme are that: after the coloring agent is diluted, the liquid level spraying technology is adopted to directly spray the coloring agent on the lawn in the dormancy stage, so that the hay can be quickly colored, the coloring time is long, the weather resistance is good, the use is convenient, and the coloring agent is suitable for large-area popularization and use.

As a further improvement, the lawn variety is festuca arundinacea.

Drawings

FIG. 1 is a flow chart of the process of the dry lawn stain of the present invention;

FIG. 2 is a graph showing the effect of various plant growth regulators on the electrolyte permeability of festuca arundinacea under low temperature stress in experimental example 1 of the present invention;

FIG. 3 shows the effect of different plant growth regulators on the chlorophyll content of festuca arundinacea under low temperature stress in experimental example 1 of the present invention;

FIG. 4 shows the effect of different plant growth regulators on festuca arundinacea proline under low temperature stress in experimental example 1 of the present invention;

FIG. 5 shows the percentage of inhibition of powdery mildew by different plant source bactericide-treated media in Experimental example 2 of the present invention.

Detailed Description

Aiming at the problem that the green period of the lawn is prolonged and the green period of the lawn is shorter when the lawn coloring agent or the lawn greening agent in the prior art is used on the lawn in the dormancy period, the invention provides a dry lawn coloring agent. The dry lawn coloring agent disclosed by the invention consists of water and the following components in percentage by mass: 0.2 to 0.3 percent of trinexapac-ethyl, 0.2 to 0.25 percent of osthole, 20 to 25 percent of ethanol, 30 to 35 percent of water-based color paste, 25 to 30 percent of water-based acrylic emulsion and 0.2 to 0.3 percent of hydroxyethyl cellulose.

According to the invention, the plant growth regulator trinexapac-ethyl is selected to improve the chlorophyll content in the lawn leaves and enhance the cold resistance of the lawn under low-temperature stress; selecting a plant source bactericide osthole (7-methoxy-8-isopentenyl coumarin) to reduce the infection probability of the lawn in the coming year; simultaneously, the types and the amounts of the solvent, the water-based color paste, the film forming agent and the thickening agent are optimized, and the physical coloring and film forming technology is applied to quickly color the lawn and resist rain wash. According to the invention, the green period of the lawn can be effectively prolonged by spraying the lawn in the dormancy period under the combined action of the two aspects of physically coloring the lawn leaves and regulating the content of chlorophyll in the lawn. The dry lawn coloring agent provided by the invention has the advantages of quick response, lasting coloring time, good weather resistance, capability of improving the safe overwintering performance of the lawn, simple use and safety and harmlessness to the lawn.

Specifically, ethanol is selected as the solvent, so that the trinexapac-ethyl and osthole (7-methoxy-8-isopentenyl coumarin) can be well dissolved, are uniformly dispersed, and have good compatibility with other components; the film forming agent is water-based acrylic emulsion, so that the coloring agent can be attached to the surface of the hay to form a film; the thickening agent is hydroxyethyl cellulose (HEC), so that the viscosity of the coloring agent can be effectively increased, and the physical stability of the medicament can be enhanced. The components are mutually matched, so that the stable performance and good water resistance of the coloring agent are ensured, and meanwhile, the film can be quickly formed on the surface of the dried grass, and the fading is further prevented.

The processing flow of the dry lawn coloring agent is shown in figure 1, and the specific preparation method comprises the following steps:

The invention screens the components and the dosage of the plant growth regulator and the plant source bactericide, and the specific description is as follows:

(1) 3 determination of Cold resistance of plant growth regulator to lawn under Low temperature stress

Selecting festuca arundinacea seeds of the cold season type lawn grass with holes with the diameter of 10cm, sowing 0.4g of each flowerpot, and culturing in a greenhouse until the four-leaf period.

Diluting with different concentrations of medicines (0.05%, 0.10%, 0.15%, 0.20%, 0.25%, and 0.30% of the effective components respectively) for 50 times, spraying with clear water as Control (CK), uniformly covering the leaves without dripping, treating 3 pots in each group, treating for 15 days, placing the tested material at-5deg.C for 12 hr, measuring electrolyte permeability (conductivity method), proline (sulfosalicylic acid method), and chlorophyll (spectrophotometry), and observing change of conductivity, proline, and chlorophyll content of the Gaofang Mao Shepian under different concentrations of medicines according to the measured data.

A. Detection of electrolyte permeability

Cutting off leaf tips and leaf stalk bases of penultimate leaves at the lower parts of plants, flushing 3 times by tap water, flushing 3 times by distilled water, finally flushing 1 time by pure water, sucking dry by using absorbent paper, weighing 1.00g of sample, filling 20mI of pure water into a test tube, stirring by using a glass rod, immersing the leaves in water completely, standing for 1h to measure initial conductivity (R ₁), boiling the materials in boiling water for 30min, cooling to room temperature, measuring final conductivity (R ₂), and calculating electrolyte permeability by using a formula (1).

R1: measuring the initial conductivity after soaking; r2: final conductivity measured after boiling.

B. Proline content determination

1) Drawing of a Standard Curve

And (I) accurately weighing 25mg of proline on an analytical balance, pouring into a small beaker, dissolving with a small amount of distilled water, pouring into a 250mL volumetric flask, adding distilled water to a certain volume to scale, and keeping the volume of 100 mug of proline per milliliter in the standard solution.

And (II) preparing the concentration of the series proline. Taking 6 50mL volumetric flasks, respectively filling 0.5mL, 1.0mL, 1.5mL, 2.0mL, 2.5mL and 3.0mL of proline stock solution, fixing the volume to a scale by distilled water, and shaking uniformly, wherein the proline concentration of each flask is respectively 1 mug/mL, 2 mug/mL, 3 mug/mL, 4 mug/mL, 5 mug/mL and 6 mug/mL.

(III) 6 test tubes were taken, and 2mL of a series of proline solutions of standard concentration and 2mL of glacial acetic acid (mass fraction 98%) and 2mL of acidic ninhydrin solution (concentration 2%, pH 5.2) were each aspirated, and each tube was heated in a boiling water bath for 30min.

And (IV) accurately adding 4mL of toluene into each test tube after cooling, oscillating for 30s, and standing for a moment to ensure that all pigments are transferred to a toluene solution.

(V) the upper proline toluene solution of each tube was gently aspirated into the cuvette with a syringe, and the cuvette was colorized at 520nm wavelength with toluene solution as a blank.

(VI) drawing a standard curve: the regression equation of the absorbance value (y) according to the proline concentration (x) is firstly obtained, then a standard curve is drawn according to the regression equation, and the proline content (mug/mL) in 2mL of measuring solution is calculated.

2) Determination of samples

Extraction of proline: accurately weighing 0.5g of plant leaves to be measured, respectively placing the plant leaves into large pipes, then respectively adding 5mL of 3% sulfosalicylic acid solution into each pipe, extracting for 10min in a boiling water bath (frequent shaking in the extraction process), cooling, and filtering in a clean test tube, wherein filtrate is proline extracting solution.

And (II) sucking 2mL of the extracting solution into another clean test tube with a glass plug, adding 2mL of glacial acetic acid and 2mL of acidic ninhydrin reagent, and heating in a boiling water bath for 30min to obtain a red solution.

(III) after cooling, adding 4mL of toluene, shaking for 30s, standing for a moment, taking the supernatant into a centrifuge tube, and centrifuging for 5min at 3000 r/min.

And (IV) lightly sucking the upper proline red toluene solution into a cuvette by using a suction tube, and colorizing at a wavelength of 520nm on a spectrophotometer by using toluene as a blank control to obtain an absorbance value.

3) Result calculation

The proline content (μg/mL) in 2mL of the assay was calculated (or looked up from the standard curve) according to the regression equation, and then the percentage of proline content in the sample was calculated. Calculated according to formula (2):

X: proline content (μg/mL) in 2mL of the measurement solution was calculated according to the regression equation.

C. chlorophyll content determination

Cleaning and drying the leaves, weighing 0.5g of the leaves, shearing, putting into a mortar, adding 2mL of acetone and a little (0.5-1 g) of quartz sand into the mortar, fully grinding, transferring into a 100mL volumetric flask, washing the mortar with acetone for multiple times, transferring the cleaning liquid into the volumetric flask, and finally fixing the volume to 100mL with acetone. After shaking sufficiently, the mixture was filtered with filter paper, and the light absorption values of the filtrate were measured at 645nm, 652nm and 663nm, respectively, with acetone as a blank. The contents of chlorophyll a and chlorophyll b and the total amount of chlorophyll were calculated according to the following formulas, respectively:

D ₆₆₃ and D ₆₄₅: representing the optical density value of the chlorophyll extract at a specified wavelength;

v: chlorophyll acetone extract final volume; m: fresh weight of leaf blade used, g.

(2) Indoor sterilization effect determination of 3 plant source bactericides on powdery mildew

Test medium (PDA): 200g of potato, 20g of glucose, 16g of agar powder, 1000mL of deionized water, sterilizing for 20min at 121 ℃ by high-pressure steam, cooling to 65 ℃ and sub-packaging for later use.

Test strain: the grass is picked from wild natural disease, and is inoculated and reproduced on a blank culture medium under the condition of a greenhouse for standby. Diameter of inoculated bacterial cake: 4mm.

The hypha growth rate method is adopted. Based on the pre-test, the tested bactericide raw medicines are prepared into PDA culture media with medicines with different concentration gradients (concentration gradient treatment: the effective component content is 0.05%, 0.10%, 0.15%, 0.20% and 0.25% respectively, and the preparation is prepared into the culture media with medicines according to the mass ratio of the medicine to the culture media=1:49), and the PDA plate added with the corresponding solvent dimethyl sulfoxide is used as a control, and each treatment is repeated for 4 times. Colony diameters were measured by the crisscross method after 5d of dark culture at 25℃and the percent inhibition of hypha growth was calculated according to the following formula (7).

Net growth = colony diameter-cake diameter … … (6)

The performance test of the dry lawn coloring agent comprises the following items of appearance, stability, water resistance, drying speed and the like, and the specific explanation is as follows:

(1) Quality evaluation

1) Appearance of

The mixed liquid is a uniform thick green liquid, a small amount of sediment or layering is allowed to exist for long-term storage, and after stirring and mixing, the mixed liquid is free of hard blocks and mechanical impurities.

2) Thermal storage stability

According to GB/T19136. Placing the sample in a centrifuge tube, placing in a constant temperature box at 54+/-2 ℃ for 14 days, and observing whether layering, precipitation and the like occur.

3) Low temperature stability

According to GB/T19137. Placing the sample in a centrifuge tube, placing in a freeze dryer at 0+ -2deg.C for 7 days, and observing whether layering and precipitation occur.

(2) Performance testing

1) Water resistance test

After the sample is diluted by 50 times by deionized water, uniformly spraying the diluted sample on a bottom plate, after the sample is completely dried, putting the test plate into a constant-temperature water bath kettle containing deionized water, soaking the test plate in water for 2/3 of the length, maintaining the water temperature at 23+/-2 ℃, taking out the test plate after soaking for 24 hours, sucking the test plate with filter paper, observing and recording whether the phenomena such as color change, bubbling, falling and the like and recovery time exist, and parallelly measuring three test plates, wherein two test plates are qualified according to the GB/T1733 medium-first method.

The results were rated on the following 5 scale:

Level 0: no obvious change exists;

stage 1: basically has no change and little bubbling, falling or discoloration;

2 stages: has certain change, obvious bubbling, falling or color change;

3 stages: severe changes, large area bubbling, shedding or discoloration;

4 stages: serious or complete failure of shedding;

wherein, the grade 0 and the grade 1 of the judgment grade are qualified, and the grade 2, the grade 3 and the grade 4 are unqualified.

2) Drying time

And diluting the sample by 50 times with deionized water, uniformly spraying on a bottom plate, placing in a constant temperature box with the temperature of 10+/-2 ℃, and recording the time from spraying to completely drying the liquid medicine, wherein the drying time is lower than 4 hours.

3) Freeze thawing stability

The sample is stirred uniformly and then is put into a plastic bottle with the volume of 500mL, the loading amount is 2/3 of that of a container, the sample tank is put into a freeze drying box with the temperature of minus 5 plus or minus 2 ℃ for 18 hours and then is taken out, and then is placed in a constant temperature box with the temperature of 23 plus or minus 2 ℃ for 6 hours, so that the whole freeze thawing cycle is realized. After 3 cycles, the vessel was opened, the sample was stirred thoroughly, and the presence or absence of hard lumps, flocculation and separation was observed. According to the A method in GB/T9268.

The present invention will be further described with reference to the following specific embodiments, and it should be noted that, on the premise of no conflict, new embodiments may be formed by any combination of the embodiments or technical features described below. The equipment and materials used, etc., are commercially available or are conventional in the art. The methods in the following examples are conventional in the art unless otherwise specified.

The raw materials used in the following examples and comparative examples are shown in table 2.

TABLE 2 raw materials for Dry lawn stains

The main instruments used in the following examples and comparative examples are shown in table 3.

TABLE 3 Main instruments

Instrument model	Name of the name	Source(s)
			XREM-100S	Cantilever type electric stirrer	New Rui Instrument factory in western city of gold altar area
SJIA-10N	Freeze dryer	Shanghai BoXie Co Ltd
			PC104	Electronic balance	Shanghai Seisaku Co., ltd
WPL-125BE	Electric heating constant temperature incubator	Test instruments Inc. of Tianjin City
			DZKW-4	Electronic constant-temperature water bath kettle	Beijing Zhongxing Wei industry instruments Co., ltd
PI type	Ultraviolet visible spectrophotometer	Shanghai Meinada instruments Co., ltd
			RXZ type of lamp	Artificial climate box	Ningbo Jiangnan Instrument factory
	Mortar with a cover	Shanghai Jie Yi electronic technology Co Ltd
				Backpack electric sprayer	Taizhou Ming-Hui electric atomizer Co., ltd

1. Specific examples of dry lawn stains in the present invention

Example 1

The dry lawn coloring agent of the embodiment consists of trinexapac-ethyl, osthole, solvent, aqueous color paste, film forming agent, thickening agent, antifreezing agent and water; the solvent is ethanol, the aqueous color paste is GTH-6030 (emerald green F4G), the film forming agent is 825 (aqueous acrylic emulsion), the thickening agent is hydroxyethyl cellulose, and the antifreezing agent is ethylene glycol. The raw material system comprises the following components in percentage by mass: trinexapac-ethyl 0.3%, osthole 0.25%, ethanol 20%, GTH-6030 30%, 825 (aqueous acrylic emulsion) 30%, hydroxyethyl cellulose 0.2%, ethylene glycol 5%, and water in balance.

The preparation method of the dry lawn coloring agent comprises the following steps:

(1) Uniformly mixing an antifreezing agent glycol and water, slowly adding a thickener hydroxyethyl cellulose under stirring, and fully dissolving for standby;

(2) The plant growth regulator trinexapac-ethyl, the plant source bactericide osthole and the solvent ethanol are accurately weighed according to the calculated dosage, added in sequence and stirred uniformly for standby;

(3) Respectively adding water-based color paste GTH-6030 (emerald green F4G) and film forming agent 825 (water-based acrylic emulsion), and uniformly stirring for later use;

Example 2

The dry lawn coloring agent of the embodiment consists of trinexapac-ethyl, osthole, solvent, aqueous color paste, film forming agent, thickening agent, antifreezing agent and water; the solvent is ethanol, the aqueous color paste is GTH-6030 (emerald green F4G), the film forming agent is 825 (aqueous acrylic emulsion), the thickening agent is hydroxyethyl cellulose, and the antifreezing agent is ethylene glycol. The raw material system comprises the following components in percentage by mass: trinexapac-ethyl 0.3%, osthole 0.25%, ethanol 20%, GTH-6030 30%, 825 (aqueous acrylic emulsion) 30%, hydroxyethyl cellulose 0.25%, ethylene glycol 5%, and water in balance.

Example 3

The dry lawn coloring agent of the embodiment consists of trinexapac-ethyl, osthole, solvent, aqueous color paste, film forming agent, thickening agent, antifreezing agent and water; the solvent is ethanol, the aqueous color paste is GTH-6030 (emerald green F4G), the film forming agent is 825 (aqueous acrylic emulsion), the thickening agent is hydroxyethyl cellulose, and the antifreezing agent is ethylene glycol. The raw material system comprises the following components in percentage by mass: trinexapac-ethyl 0.3%, osthole 0.25%, ethanol 20%, GTH-6030 30%, 825 (aqueous acrylic emulsion) 30%, hydroxyethyl cellulose 0.3%, ethylene glycol 5%, and water in balance.

Example 4

The dry lawn coloring agent of the embodiment consists of trinexapac-ethyl, osthole, a solvent, aqueous color paste, a film forming agent, a thickening agent, a wetting agent, an antifreezing agent and water; the solvent is ethanol, the aqueous color paste is GTH-6030 (emerald green F4G), the film forming agent is 825 (aqueous acrylic emulsion), the thickening agent is hydroxyethyl cellulose, the wetting agent is OP-10 (octylphenol polyoxyethylene ether), and the antifreezing agent is ethylene glycol. The raw material system comprises the following components in percentage by mass: 0.3% of trinexapac-ethyl, 0.25% of osthole, 20% of ethanol, 30% of GTH-6030 30%, 825 (aqueous acrylic emulsion), 0.2% of hydroxyethyl cellulose, 1% of OP-10 (octylphenol polyoxyethylene ether), 5% of ethylene glycol and the balance of water.

(2) The plant growth regulator trinexapac-ethyl, the plant source bactericide osthole, the wetting agent OP-10 (octyl phenol polyoxyethylene ether) and the solvent ethanol are weighed accurately according to the calculated dosage, added in sequence and stirred uniformly for standby;

Example 5

The dry lawn coloring agent of the embodiment consists of trinexapac-ethyl, osthole, a solvent, aqueous color paste, a film forming agent, a thickening agent, a wetting agent, an antifreezing agent and water; the solvent is ethanol, the aqueous color paste is GTH-6030 (emerald green F4G), the film forming agent is 825 (aqueous acrylic emulsion), the thickening agent is hydroxyethyl cellulose, the wetting agent is OP-10 (octylphenol polyoxyethylene ether), and the antifreezing agent is ethylene glycol. The raw material system comprises the following components in percentage by mass: 0.3% of trinexapac-ethyl, 0.25% of osthole, 20% of ethanol, 30% of GTH-6030 30%, 825 (aqueous acrylic emulsion), 0.2% of hydroxyethyl cellulose, 0.5% of OP-10 (octylphenol polyoxyethylene ether), 5% of ethylene glycol and the balance of water.

Example 6

The dry lawn coloring agent of the embodiment consists of trinexapac-ethyl, osthole, a solvent, aqueous color paste, a film forming agent, a thickening agent, a wetting agent, an antifreezing agent and water; the solvent is ethanol, the aqueous color paste is GTH-6030 (emerald green F4G), the film forming agent is 825 (aqueous acrylic emulsion), the thickening agent is hydroxyethyl cellulose, the wetting agent is OP-10 (octylphenol polyoxyethylene ether), and the antifreezing agent is 1, 2-propylene glycol. The raw material system comprises the following components in percentage by mass: 0.3% of trinexapac-ethyl, 0.25% of osthole, 20% of ethanol, 30% of GTH-6030 30%, 825 (aqueous acrylic emulsion), 0.2% of hydroxyethyl cellulose, 1% of OP-10 (octylphenol polyoxyethylene ether), 1, 2-propylene glycol and the balance of water.

(1) Uniformly mixing an antifreezing agent 1, 2-propanediol and water, slowly adding a thickener hydroxyethyl cellulose under stirring, and fully dissolving for standby;

Example 7

The dry lawn coloring agent of the embodiment consists of trinexapac-ethyl, osthole, a solvent, aqueous color paste, a film forming agent, a thickening agent, a wetting agent, an antifreezing agent and water; the solvent is ethanol, the aqueous color paste is GTH-6030 (emerald green F4G), the film forming agent is 825 (aqueous acrylic emulsion), the thickening agent is hydroxyethyl cellulose, the wetting agent is OP-10 (octylphenol polyoxyethylene ether), and the antifreezing agent is 1, 2-propylene glycol. The raw material system comprises the following components in percentage by mass: 0.3% of trinexapac-ethyl, 0.25% of osthole, 20% of ethanol, 30% of GTH-6030 30%, 825 (aqueous acrylic emulsion), 0.2% of hydroxyethyl cellulose, 1% of OP-10 (octylphenol polyoxyethylene ether), 3% of 1, 2-propylene glycol and the balance of water.

Example 8

The dry lawn coloring agent of the embodiment consists of trinexapac-ethyl, osthole, a solvent, aqueous color paste, a film forming agent, a thickening agent, a wetting agent, an antifreezing agent and water; the solvent is ethanol, the aqueous color paste is GTH-6030 (emerald green F4G), the film forming agent is 825 (aqueous acrylic emulsion), the thickening agent is hydroxyethyl cellulose, the wetting agent is OP-10 (octylphenol polyoxyethylene ether), and the antifreezing agent is 1, 2-propylene glycol. The raw material system comprises the following components in percentage by mass: 0.3% of trinexapac-ethyl, 0.25% of osthole, 20% of ethanol, 30% of GTH-6030 30%, 825 (aqueous acrylic emulsion), 0.2% of hydroxyethyl cellulose, 1% of OP-10 (octylphenol polyoxyethylene ether), 5% of 1, 2-propylene glycol and the balance of water.

Example 9

The dry lawn coloring agent of the embodiment consists of trinexapac-ethyl, osthole, a solvent, aqueous color paste, a film forming agent, a thickening agent, a wetting agent, an antifreezing agent and water; the solvent is ethanol, the aqueous color paste is GTH-6030 (emerald green F4G), the film forming agent is 825 (aqueous acrylic emulsion), the thickening agent is hydroxyethyl cellulose, the wetting agent is OP-10 (octylphenol polyoxyethylene ether), and the antifreezing agent is ethylene glycol. The raw material system comprises the following components in percentage by mass: 0.3% of trinexapac-ethyl, 0.25% of osthole, 20% of ethanol, 30% of GTH-6030 30%, 825 (aqueous acrylic emulsion), 0.2% of hydroxyethyl cellulose, 1% of OP-10 (octylphenol polyoxyethylene ether), 1% of ethylene glycol and the balance of water.

Example 10

The dry lawn coloring agent of the embodiment consists of trinexapac-ethyl, osthole, a solvent, aqueous color paste, a film forming agent, a thickening agent, a wetting agent, an antifreezing agent and water; the solvent is ethanol, the aqueous color paste is GTH-6030 (emerald green F4G), the film forming agent is 825 (aqueous acrylic emulsion), the thickening agent is hydroxyethyl cellulose, the wetting agent is OP-10 (octylphenol polyoxyethylene ether), and the antifreezing agent is ethylene glycol. The raw material system comprises the following components in percentage by mass: 0.3% of trinexapac-ethyl, 0.25% of osthole, 20% of ethanol, 30% of GTH-6030 30%, 825 (aqueous acrylic emulsion), 0.2% of hydroxyethyl cellulose, 1% of OP-10 (octylphenol polyoxyethylene ether), 3% of ethylene glycol and the balance of water.

Example 11

The dry lawn coloring agent of the embodiment consists of trinexapac-ethyl, osthole, a solvent, aqueous color paste, a film forming agent, a thickening agent, a wetting agent, an antifreezing agent, a preservative, a defoaming agent and water; the solvent is ethanol, the aqueous color paste is GTH-6030 (emerald green F4G), the film forming agent is 825 (aqueous acrylic emulsion), the thickening agent is hydroxyethyl cellulose, the wetting agent is OP-10 (octyl phenol polyoxyethylene ether), the antifreezing agent is 1, 2-propanediol, the preservative is kasong, and the defoaming agent is AF1501. The raw material system comprises the following components in percentage by mass: 0.2% of trinexapac-ethyl, 0.25% of osthole, 25% of ethanol, 25% of GTH-6030 35% of 825 (aqueous acrylic emulsion), 0.2% of hydroxyethyl cellulose, 0.5% of OP-10, 3% of 1, 2-propylene glycol, 0.1% of pinocembrin, 0.3% of AF1501 and the balance of water.

(3) Adding water-based color paste GTH-6030 (emerald green F4G), film forming agent 825, antiseptic Kathon and defoaming agent AF1501 respectively, and stirring uniformly for standby;

Example 12

The dry lawn coloring agent of the embodiment consists of trinexapac-ethyl, osthole, a solvent, aqueous color paste, a film forming agent, a thickening agent, a wetting agent, an antifreezing agent, a preservative, a defoaming agent and water; the solvent is ethanol, the aqueous color paste is GTH-6030 (emerald green F4G), the film forming agent is 825 (aqueous acrylic emulsion), the thickening agent is hydroxyethyl cellulose, the wetting agent is OP-10 (octyl phenol polyoxyethylene ether), the antifreezing agent is 1, 2-propanediol, the preservative is kasong, and the defoaming agent is AF1501. The raw material system comprises the following components in percentage by mass: 0.25% of trinexapac-ethyl, 0.2% of osthole, 20% of ethanol, 20% of GTH-6030 30%, 825 (aqueous acrylic emulsion) 30%, 0.25% of hydroxyethyl cellulose, 10% of OP-1%, 5% of 1, 2-propylene glycol, 0.2% of pinocembrin, 0.5% of AF1501 and the balance of water.

Example 13

The dry lawn coloring agent of the embodiment consists of trinexapac-ethyl, osthole, a solvent, aqueous color paste, a film forming agent, a thickening agent, a wetting agent, an antifreezing agent, a preservative, a defoaming agent and water; the solvent is ethanol, the aqueous color paste is GTH-6030 (emerald green F4G), the film forming agent is 825 (aqueous acrylic emulsion), the thickening agent is hydroxyethyl cellulose, the wetting agent is OP-10 (octyl phenol polyoxyethylene ether), the antifreezing agent is glycol, the preservative is kasong, and the defoaming agent is AF1501. The raw material system comprises the following components in percentage by mass: 0.3% of trinexapac-ethyl, 0.25% of osthole, 25% of ethanol, 30% of GTH-6030 30%, 825 (aqueous acrylic emulsion), 0.3% of hydroxyethyl cellulose, 0.5% of OP-10, 3% of ethylene glycol, 0.3% of pinocembrane, 0.3% of AF1501 and the balance of water.

Example 14

The dry lawn coloring agent of the embodiment consists of trinexapac-ethyl, osthole, a solvent, aqueous color paste, a film forming agent, a thickening agent, a wetting agent, an antifreezing agent, a preservative, a defoaming agent and water; the solvent is ethanol, the aqueous color paste is GTH-6030 (emerald green F4G), the film forming agent is 825 (aqueous acrylic emulsion), the thickening agent is hydroxyethyl cellulose, the wetting agent is OP-10 (octyl phenol polyoxyethylene ether), the antifreezing agent is glycol, the preservative is kasong, and the defoaming agent is AF1501. The raw material system comprises the following components in percentage by mass: 0.2% of trinexapac-ethyl, 0.2% of osthole, 20% of ethanol, 25% of GTH-6030 35%, 825 (aqueous acrylic emulsion), 0.25% of hydroxyethyl cellulose, 10-OP, 5% of glycol, 0.1% of pinocembrin, 0.5% of AF1501 and the balance of water.

Example 15

The dry lawn coloring agent of the embodiment consists of trinexapac-ethyl, osthole, a solvent, aqueous color paste, a film forming agent, a thickening agent, a wetting agent, an antifreezing agent, a preservative, a defoaming agent and water; the solvent is ethanol, the aqueous color paste is GTH-6030 (emerald green F4G), the film forming agent is 825 (aqueous acrylic emulsion), the thickening agent is hydroxyethyl cellulose, the wetting agent is OP-10 (octyl phenol polyoxyethylene ether), the antifreezing agent is 1, 2-propanediol, the preservative is kasong, and the defoaming agent is AF1501. The raw material system comprises the following components in percentage by mass: 0.25% of trinexapac-ethyl, 0.2% of osthole, 25% of ethanol, 30% of GTH-6030 35%, 825 (aqueous acrylic emulsion), 0.3% of hydroxyethyl cellulose, 0.5% of OP-10, 3% of 1, 2-propylene glycol, 0.2% of pinocembrin, 0.3% of AF1501 and the balance of water.

Example 16

The dry lawn coloring agent of the embodiment consists of trinexapac-ethyl, osthole, a solvent, aqueous color paste, a film forming agent, a thickening agent, a wetting agent, an antifreezing agent, a preservative, a defoaming agent and water; the solvent is ethanol, the aqueous color paste is GTH-6030 (emerald green F4G), the film forming agent is 825 (aqueous acrylic emulsion), the thickening agent is hydroxyethyl cellulose, the wetting agent is OP-10 (octyl phenol polyoxyethylene ether), the antifreezing agent is glycol, the preservative is kasong, and the defoaming agent is AF1501. The raw material system comprises the following components in percentage by mass: 0.3% of trinexapac-ethyl, 0.25% of osthole, 20% of ethanol, 25% of GTH-6030 30%, 825 (aqueous acrylic emulsion), 0.2% of hydroxyethyl cellulose, 10-OP, 5% of glycol, 0.3% of pinocembrin, 0.5% of AF1501 and the balance of water.

2. Experimental example application of dry lawn coloring agent in prolonging green period of winter lawn

Experimental example 1

The experiment example shows that the cold resistance of different plant growth regulators to the lawn under low temperature stress can be obviously changed in various physiological indexes such as plasma membrane permeability, defense system enzyme activity, photosynthesis and the like of the lawn under low temperature stress. The mechanism of regulating the frost resistance of the cold-season turf grass by the plant growth regulator mainly comprises the steps of improving the enzyme activity of a plant defense system, increasing the content of intracellular penetration regulating substances such as soluble sugar, proline, protein and the like, and finally moderately inhibiting or delaying the growth of the turf grass and improving the frost resistance of the turf grass.

At present, two approaches for improving the cold resistance of turf grass are as follows: firstly, the frost resistance of the turf grass is improved by selecting high-quality turf grass varieties and carrying out reasonable water and fertilizer management; secondly, the plant frost resistance is improved by using plant growth regulator. The invention starts from the physiological index change of plants under low temperature stress, analyzes the regulating effect of trinexapac-ethyl, paclobutrazol and uniconazole on the frost resistance of the cold-season turf grass, selects proper plant growth regulator types and the dosage thereof through the change of electrolyte permeability, proline and chlorophyll content of festuca arundinacea under low temperature stress, and the experimental method is implemented in the specific implementation according to the specific operation process, and the experimental result is as follows.

(1) Influence of different agents on electrolyte permeability of festuca arundinacea under low-temperature stress

As can be seen from fig. 2, the electrolyte permeability of festuca arundinacea under low temperature stress can be effectively reduced after the treatment of the three agents, and the electrolyte permeability of festuca arundinacea gradually decreases with the increase of the agent concentration within a certain concentration range. The electrolyte permeability decreasing rate of the trinexapac-ethyl begins to decrease greatly when the concentration of the medicament increases to a certain concentration, wherein the electrolyte permeability decreasing rate of the trinexapac-ethyl begins to decrease greatly when the concentration is more than 0.2%; the electrolyte permeability reduction rate of paclobutrazol begins to be greatly reduced when the concentration is more than 0.15%; the electrolyte permeability decrease rate of uniconazole begins to decrease greatly at a concentration of more than 0.1%. The electrolyte permeability is selected to be less than 20% as an alternative, so the preferred agent is trinexapac-ethyl at a concentration of 0.2% to 0.3%.

(2) Influence of different agents on the chlorophyll content of festuca arundinacea under low-temperature stress

As can be seen from fig. 3, the chlorophyll content of festuca arundinacea under low temperature stress can be effectively improved after the treatment of the three agents, and the chlorophyll content of festuca arundinacea gradually increases with the increase of the agent concentration within a certain concentration range. The chlorophyll content increasing rate of the trinexapac-ethyl begins to be greatly reduced when the concentration of the medicament is increased to a certain concentration, wherein the chlorophyll content increasing rate of the trinexapac-ethyl begins to be greatly reduced when the concentration is more than 0.2%; the chlorophyll content increasing rate of the paclobutrazol begins to be greatly reduced when the concentration is more than 0.15%; the rate of increase of chlorophyll content of uniconazole begins to decrease greatly at a concentration of more than 0.1%. Selecting a chlorophyll content of more than 1.65mg/g as an alternative, wherein the preferable medicament is trinexapac-ethyl, and the concentration is 0.15% -0.3%; or paclobutrazol with the concentration of 0.2% -0.3%.

(3) Influence of different agents on proline content of festuca arundinacea under low-temperature stress

As can be seen from fig. 4, the proline content of festuca arundinacea under low temperature stress can be effectively increased after the treatment of the three agents, and the proline content is increased and then decreased with the increase of the agent concentration within a certain concentration range. Wherein the trinexapac-ethyl has the highest proline content at a concentration of 0.2%; paclobutrazol has the highest proline content at the concentration of 0.15%; the uniconazole has the highest proline content at a concentration of 0.1%. Alternatively, the proline content is greater than 600 μg/g, preferably the agent is trinexapac-ethyl at a concentration of 0.15% -0.3%.

In conclusion, the three selected plant growth regulators have the effect of improving the cold resistance of the festuca arundinacea, and the comprehensive preferable trinexapac-ethyl concentration is 0.2% -0.3%.

Experimental example 2

Compared with chemical pesticides, the biological pesticide has the advantages of quick degradation, low toxicity, difficult generation of drug resistance and difficult generation of harmful substances, is environment-friendly, and adopts three biological pesticides of osthole, allicin and phenytoin to carry out indoor biological test on powdery mildew-prone bacteria of a lawn.

As can be seen from fig. 5, the three agents all have an inhibitory effect on powdery mildew, and the percentage of inhibition of powdery mildew gradually increases with increasing concentration of the agents within a certain concentration range. The percentage increase rate of inhibiting the powdery mildew starts to be greatly reduced when the concentration of the medicament is increased to a certain concentration, wherein the percentage increase rate of inhibiting the powdery mildew starts to be greatly reduced when the concentration of osthole is more than 0.1%; the percent of inhibiting the powdery mildew of the allicin starts to be greatly reduced when the concentration of the allicin is more than 0.15%; the percent inhibition of powdery mildew by phenytoin begins to drop greatly at concentrations greater than 0.05%. Alternatively, the percent inhibition is greater than 90%, so the preferred agent is osthole.

In conclusion, the three selected biopesticides have inhibition effect on powdery mildew bacteria, and the comprehensive preferred osthole has the concentration of 0.2% -0.25%.

Experimental example 3

The experimental example illustrates the effect of different solvents and amounts on the stability of the dry lawn stain. The plant growth regulator is trinexapac-ethyl, and the dosage is 0.3%; the biological pesticide selects osthole, the dosage is 0.25%; the appropriate solvent type and amount is determined by varying the type and amount of solvent, and determining the effect on the stability of the agent.

(1) Selection of solvent species

Table 4 shows the effect of different solvents on the stability of the stain. The test results are shown in Table 5.

TABLE 4 influence of different solvents on the stability of the stain (formulation 1# -4#, each component is mass percent/%)

TABLE 5 influence of different solvent types on the stability of the stain

As shown in Table 5, except that the liquid separation of the small sample No. 1 was serious, the small sample No.2 and No. 4 were uniformly layered, the floating color was serious, and the appearance color of the preparation was not uniform, so that the solvent ethanol was preferred as an alternative for the next screening.

(2) Selection of the amount of solvent

Table 6 shows the effect of different amounts of solvent on the stability of the stain. The test results are shown in Table 7.

TABLE 6 influence of different amounts of solvents on the stability of the stain (formulation 5# -8#, each component is mass percent/%)

TABLE 7 influence of different solvent amounts on the stability of the dyestuffs

As can be seen from Table 7, except that the liquid separation of the pilot runs 1# and 7# was severe, the liquid separation of the pilot runs 5# and 6# was severe and the liquid level was oil-separating, and the pilot run 8# was left to stand without fluidity. Therefore, the ethanol dosage of the solvent is preferably 20-25 percent, and the next screening is carried out.

Experimental example 4

The experimental example illustrates the influence of different aqueous color pastes and the dosage on the stability of the dry lawn coloring agent. The plant growth regulator is trinexapac-ethyl, and the dosage is 0.3%; the biological pesticide selects osthole, the dosage is 0.25%; ethanol is selected as the solvent, and the dosage is 0.20%; the optimal type and amount of the aqueous color paste are determined by changing the type and amount of the aqueous color paste and measuring the influence on the stability of the medicament.

(1) Selection of the type of aqueous color paste

Table 8 shows the effect of different aqueous color pastes on the stability of the stain. The test results are shown in Table 9.

Table 8 influence of different aqueous color paste types on the stability of the colorant (formula 9#, each component is mass percent/%)

TABLE 9 influence of different aqueous mill base types on the stability of the stain

As can be seen from Table 9, the liquid separation of the bench scale No.1 is serious; the small test No. 9 is layered, the flooding is serious, and the appearance color of the medicament is nonuniform; therefore, the water-based color paste GTH-6030 is preferable as an alternative for the next screening.

(2) Selection of the amount of the aqueous color paste

Table 10 shows the effect of different amounts of aqueous color paste on the stability of the colorant. The results of the detection are shown in Table 11.

Table 10 influence of different amounts of aqueous color paste on stability of the colorant (formula 10# -12#, each component is mass percent/%)

Table 11 influence of different aqueous color paste usage on stain stability

As can be seen from Table 11, the liquid separation of the test Nos. 1, 10 and 11 was severe, and the test No. 12 was free from fluidity in heat storage, wherein the test No. 10 was diluted 50 times with water and color was compared to color difference. Therefore, the dosage of the water-based color paste GTH-6030 is preferably 30-35%, and the next screening is carried out.

Experimental example 5

The experimental example illustrates the effect of different film forming agents and the dosage on the stability of the dry lawn coloring agent. The plant growth regulator is trinexapac-ethyl, and the dosage is 0.3%; the biological pesticide selects osthole, the dosage is 0.25%; ethanol is selected as the solvent; the aqueous color paste selects GTH-6030, and the optimal film former type and dosage are determined by changing the film former type and dosage and measuring the influence on the stability of the medicament.

(1) Selection of film former species

Table 12 shows the effect of different film formers on the stability of the stain. The results of the detection are shown in Table 13.

TABLE 12 influence of different film Forming agent types on stability of the formulations (formulation 13# -15#, each component is mass percent/%)

TABLE 13 influence of different film former species on agent stability

As can be seen from Table 13, the liquid separation of the pilot scale No. 1 is serious; the small test 13# 15# is uniformly layered, has serious flooding and nonuniform medicament appearance color; film former 825 is preferred as an alternative for further screening.

(2) Selection of the amount of film Forming agent

Table 14 shows the effect of different film former amounts on the stability of the stain. The test results are shown in Table 15.

TABLE 14 influence of different film Forming Agents on stability and Water resistance of the formulations (formulation 16# -18#, each component is mass percent/%)

TABLE 15 influence of different film former amounts on agent stability and Water resistance

As can be seen from Table 15, the liquid separation of the runs 1#, 16# and 17# was severe, and the run 18# was heat-stored and free of fluidity at low temperature, with the water resistance of run 16# being grade 2. Therefore, the film former 825 is preferably used in an amount of 25% to 30% for further screening.

Experimental example 6

The experimental example illustrates the effect of different thickeners and amounts on the stability of the dry lawn stain. The plant growth regulator is trinexapac-ethyl, and the dosage is 0.3%; the biological pesticide selects osthole, the dosage is 0.25%; ethanol is selected as the solvent; the water-based color paste is selected from GTH-6030; film former selection 825; the optimum thickener type and amount was determined by varying the thickener type and amount, and determining the effect on the stability of the agent.

(1) Selection of thickener classes

Table 16 shows the effect of different thickeners on the stability of the stain. The results of the detection are shown in Table 17.

TABLE 16 influence of different thickener classes on stability of the formulations (formulation 19# -20#, each component is mass percent/%)

Note that: formulation 19# is the formulation of the dry lawn stain of example 1.

TABLE 17 influence of different thickener classes on the stability of pharmaceutical agents

As can be seen from Table 17, the stability of the pilot scale # 19 was acceptable; no flowability of pilot run No. 20; the thickener Hydroxyethylcellulose (HEC) is preferred as an alternative for the next screening.

(2) Selection of thickener dosage

Table 18 shows the effect of different amounts of thickener on the stability of the stain. The results of the detection are shown in Table 19.

TABLE 18 influence of different thickener dosages on the stability of the formulations (formulation 21# -24#, each component is mass percent/%)

Note that: formulas 22# and 23# are the formulas of the dry lawn stains of example 2 and example 3.

TABLE 19 influence of different thickener dosages on the stability of the medicaments

As can be seen from Table 19, the physical stability of each of the trials # 19, # 22 and # 23 was acceptable, the trial # 21 had a small amount of liquid out, and the trial # 24 had no fluidity in heat storage. The thickener hydroxyethyl cellulose (HEC) is preferably used in an amount of 0.2% -0.3% for the next screening.

Experimental example 7

The experimental example illustrates the effect of different wetting agents and amounts on the stability of the dry lawn stain. The plant growth regulator is trinexapac-ethyl, and the dosage is 0.3%; the biological pesticide selects osthole, the dosage is 0.25%; ethanol is selected as the solvent; the water-based color paste is selected from GTH-6030; film former selection 825; the thickener is hydroxyethyl cellulose (HEC); the optimum wetting agent type and amount is determined by varying the wetting agent type and amount, and determining the effect on the stability of the agent.

(1) Selection of wetting agent species

Table 20 shows the effect of different wetting agents on the stability of the stain. The results of the detection are shown in Table 21.

TABLE 20 influence of different wetting agent types on stability of the formulations (formulation 25# -26#, each component is mass percent/%)

Note that: formulation 26 is the formulation of the dry lawn stain of example 4.

TABLE 21 influence of different wetting agent classes on the stability of the medicament

(2) Selection of the amount of wetting agent

Table 22 shows the effect of different amounts of wetting agent on the stability of the dye. The results of the detection are shown in Table 23.

Table 22 influence of different wetting agent amounts on the stability of the formulations (formulation 27# -29#, each component is mass percent/%)

Note that: formulation 27# is the formulation of the dry lawn stain of example 5.

TABLE 23 influence of different wetting agent amounts on the stability of the medicaments

As can be seen from Table 23, both the physical stability of the pilot run No. 26 and the physical stability of the pilot run No. 27 were satisfactory, and the pilot run No. 28 and the pilot run No. 29 had a small amount of liquid. Therefore, the amount of the wetting agent OP-10 is preferably 0.5% -1% for the next screening.

Experimental example 8

The experimental example illustrates the influence of different antifreezing agents and the dosage on the stability of the dry lawn coloring agent. The plant growth regulator is trinexapac-ethyl, and the dosage is 0.3%; the biological pesticide selects osthole, the dosage is 0.25%; ethanol is selected as the solvent; the water-based color paste is selected from GTH-6030; film former selection 825; the thickener is hydroxyethyl cellulose (HEC); the wetting agent is OP-10; the optimal antifreeze type and amount is determined by varying the antifreeze type and amount, and determining the effect on the stability of the agent.

Table 24 shows the effect of different antifreeze types and amounts on the stability of the formulations. The test results are shown in Table 25.

TABLE 24 influence of different antifreeze types and amounts on the stability of the formulations (formulation 30# -36#, each component is mass percent/%)

Note that: formulas 30# to 32# are formulas of the dry lawn stains in examples 6 to 8, and formulas 34# and 35# are formulas of the dry lawn stains in examples 9 and 10.

TABLE 25 influence of different antifreeze types and amounts on the stability of the medicaments

As can be seen from Table 25, the physical stability of the pilot plant No. 26, no. 31, no. 32, no. 35 and No. 36 are qualified; the low temperatures of the pilot scale 30# and 34# are frozen; small amounts of liquid precipitation phenomenon exists in the small test pieces 33# and 36 #; therefore, the antifreezing agent ethylene glycol and 1, 2-propylene glycol can be used in 3-5% preferably.

Comprehensive experimental examples 3 to 8, preferred combination formulas were obtained as follows:

Selecting trinexapac-ethyl as a plant growth regulator through screening; the plant source bactericide is osthole; ethanol is selected as the solvent; the water-based color paste is selected from GTH-6030; film former selection 825; the thickener is hydroxyethyl cellulose (HEC); the wetting agent is OP-10; the antifreezing agent is selected from ethylene glycol or 1, 2-propylene glycol, and the influence of different combination formulas on the stability of the medicament is tested. The experimental design is shown in Table 26, and the experimental results are shown in tables 27 and 28.

Table 26 preferably shows the formulation combinations (formulation 37# -42#, each component is mass percent/%)

Note that: formulas 37# to 42# are the formulas of the dry lawn stains in examples 11 to 16.

Table 27 effects of different combination formulations on formulation stability and permeability

As can be seen from Table 27, the physical stability of the preferred combination pilot scale is acceptable.

Table 28 preferred combination recipe performance measurement results

As can be seen from Table 28, the properties of the preferred combination formulation meet the desired requirements.

Experimental example 9

The experimental examples carried out field tests on the dry lawn stains of the preferred formulas 37# to 42# (i.e. examples 11 to 16) to evaluate the actual application effect, and the specific implementation process is as follows:

(1) Test method

2022, 11, 18 Am 10:00 is carried out in Zhengzhou West flow lake park, and the lawn variety festuca arundinacea is adopted; the medicament is a preferred combination formula (pilot scale # 37, 38#, 39#, 40#, 41#, 42 #); concentration of drug used: diluting with water for 50 times. Selecting susceptible areas (powdery mildew bacteria are the main) in the past year, wherein each treatment area is 10 square meters, and spraying by using clear water as a contrast and using a backpack electric sprayer, wherein the spraying is based on the condition that the blades are uniformly covered without dripping.

(2) Survey item

The preliminary investigation is carried out 4 hours after the application, whether the liquid medicine is completely dried or not is recorded, whether the appearance of the lawn has chromatic aberration or not is recorded, the change of the appearance of the lawn is observed every 10 days before, and the coloring duration is recorded. Samples were taken at random at day 1 of 12 of 2022 to determine the electrolyte permeability, chlorophyll content and proline content of the lawn. Again, the investigation was conducted at 4/8/2023 to determine whether the grass was turned green, and the area of disease occurrence in each cell was measured and recorded using a 10cm×10cm square grid, and the disease incidence and control effect were calculated according to the following formula.

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(3) Test results

The practical use effects are shown in table 29, and as can be seen from the table, all experimental groups have good use effects, uniform coloring, no color difference with the lawn, lasting time of more than two months, and no influence on normal turning green of the lawn in the next year.

Table 29 effect of actual use

The cold resistance and disease resistance of the dried lawns are shown in table 30, and compared with a clear water control group, the experiment group can obviously reduce the electrolyte permeability of the tall thatch under low-temperature stress, improve the chlorophyll content and the proline content of the tall thatch, and obviously improve the cold resistance of the lawns; the control effect of the experimental groups is over 91.27%, and the lawns can be well protected from being infected by germs.

Table 30 influence on cold resistance and disease resistance of lawns

The above description is only a preferred embodiment of the present invention, and the patent protection scope of the present invention is defined by the claims, and all equivalent structural changes made by the specification and the drawings of the present invention should be included in the protection scope of the present invention.

Claims

1. A dry lawn coloring agent, which is characterized in that: the dry lawn coloring agent consists of water, a wetting agent, an auxiliary agent and the following components in percentage by mass: 0.2 to 0.3 percent of trinexapac-ethyl, 0.2 to 0.25 percent of osthole, 20 to 25 percent of ethanol, 30 to 35 percent of water-based color paste and 25 to 30 percent of water-based acrylic emulsion.

2. The dry lawn stain of claim 1, wherein the dry lawn stain is a white lawn stain. The wetting agent is octyl phenol polyoxyethylene ether; the mass percentage of the wetting agent in the coloring agent is 0.5-1%.

3. The dry lawn stain of claim 1, wherein the dry lawn stain is a white lawn stain. The auxiliary agent comprises a thickening agent; the mass percentage of the thickener in the coloring agent is 0.2-0.3%.

4. A dry lawn stain according to claim 3, wherein: the thickener is hydroxyethyl cellulose.

5. The dry lawn stain of claim 1, wherein the dry lawn stain is a white lawn stain. The auxiliary agent comprises an antifreezing agent; the mass percentage of the antifreezing agent in the coloring agent is 1-5%.

6. The dry lawn stain of claim 5, wherein the dry lawn stain is a white lawn stain. The antifreezing agent is one of ethylene glycol and 1, 2-propylene glycol.

7. A dry lawn stain according to any of claims 1-6, wherein: the auxiliary agent comprises one or two of an antifoaming agent and a preservative; the mass percentage of the defoaming agent in the coloring agent is 0.3-0.5%; the mass percentage of the preservative in the coloring agent is 0.1-0.3%.

8. Use of a dry lawn stain according to any one of claims 1 to 7 for extending the green phase of winter lawns.

9. The use of a dry lawn stain of claim 8 for extending the green period of winter lawns, wherein: the diluted dye is sprayed on the lawn in the dormancy stage.

10. Use of a dry lawn stain according to claim 8 or 9 for extending the green period of winter lawns, characterised in that: the lawn variety is festuca arundinacea.