CN118217268A - Aquatic insecticide containing hexaflumuron and application thereof - Google Patents
Aquatic insecticide containing hexaflumuron and application thereof Download PDFInfo
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- CN118217268A CN118217268A CN202410075552.2A CN202410075552A CN118217268A CN 118217268 A CN118217268 A CN 118217268A CN 202410075552 A CN202410075552 A CN 202410075552A CN 118217268 A CN118217268 A CN 118217268A
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- CN
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- Prior art keywords
- hexaflumuron
- aquatic
- synergist
- carvacrol
- fish
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- RGNPBRKPHBKNKX-UHFFFAOYSA-N hexaflumuron Chemical compound C1=C(Cl)C(OC(F)(F)C(F)F)=C(Cl)C=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F RGNPBRKPHBKNKX-UHFFFAOYSA-N 0.000 title claims abstract description 38
- 239000002917 insecticide Substances 0.000 title claims abstract description 12
- 241000251468 Actinopterygii Species 0.000 claims abstract description 28
- HHTWOMMSBMNRKP-UHFFFAOYSA-N carvacrol Natural products CC(=C)C1=CC=C(C)C(O)=C1 HHTWOMMSBMNRKP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 235000007746 carvacrol Nutrition 0.000 claims abstract description 18
- WYXXLXHHWYNKJF-UHFFFAOYSA-N isocarvacrol Natural products CC(C)C1=CC=C(O)C(C)=C1 WYXXLXHHWYNKJF-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000004480 active ingredient Substances 0.000 claims abstract description 15
- 150000007822 carvacrol derivatives Chemical group 0.000 claims abstract description 3
- 239000000575 pesticide Substances 0.000 claims description 19
- 244000045947 parasite Species 0.000 claims description 11
- 239000000839 emulsion Substances 0.000 claims description 8
- 241000238424 Crustacea Species 0.000 claims description 4
- 239000004530 micro-emulsion Substances 0.000 claims description 2
- 239000002671 adjuvant Substances 0.000 claims 1
- 239000006071 cream Substances 0.000 claims 1
- RECUKUPTGUEGMW-UHFFFAOYSA-N carvacrol Chemical compound CC(C)C1=CC=C(C)C(O)=C1 RECUKUPTGUEGMW-UHFFFAOYSA-N 0.000 abstract description 15
- 230000000694 effects Effects 0.000 abstract description 15
- 238000002360 preparation method Methods 0.000 abstract description 10
- 238000009360 aquaculture Methods 0.000 abstract description 7
- 244000144974 aquaculture Species 0.000 abstract description 7
- 239000012747 synergistic agent Substances 0.000 abstract description 6
- 231100000674 Phytotoxicity Toxicity 0.000 abstract description 3
- 235000019688 fish Nutrition 0.000 description 26
- 238000012360 testing method Methods 0.000 description 19
- 239000003814 drug Substances 0.000 description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 12
- 241000238631 Hexapoda Species 0.000 description 10
- 241000252230 Ctenopharyngodon idella Species 0.000 description 7
- 239000003795 chemical substances by application Substances 0.000 description 6
- QYDYPVFESGNLHU-UHFFFAOYSA-N elaidic acid methyl ester Natural products CCCCCCCCC=CCCCCCCCC(=O)OC QYDYPVFESGNLHU-UHFFFAOYSA-N 0.000 description 6
- QYDYPVFESGNLHU-KHPPLWFESA-N methyl oleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC QYDYPVFESGNLHU-KHPPLWFESA-N 0.000 description 6
- 229940073769 methyl oleate Drugs 0.000 description 6
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 6
- 229920000053 polysorbate 80 Polymers 0.000 description 6
- HRYILSDLIGTCOP-UHFFFAOYSA-N N-benzoylurea Chemical compound NC(=O)NC(=O)C1=CC=CC=C1 HRYILSDLIGTCOP-UHFFFAOYSA-N 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 241000883306 Huso huso Species 0.000 description 4
- 208000030852 Parasitic disease Diseases 0.000 description 4
- 238000012937 correction Methods 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 4
- 230000003071 parasitic effect Effects 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 239000005893 Diflubenzuron Substances 0.000 description 3
- QQQYTWIFVNKMRW-UHFFFAOYSA-N diflubenzuron Chemical compound FC1=CC=CC(F)=C1C(=O)NC(=O)NC1=CC=C(Cl)C=C1 QQQYTWIFVNKMRW-UHFFFAOYSA-N 0.000 description 3
- 229940019503 diflubenzuron Drugs 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 230000001846 repelling effect Effects 0.000 description 3
- WCXDHFDTOYPNIE-RIYZIHGNSA-N (E)-acetamiprid Chemical compound N#C/N=C(\C)N(C)CC1=CC=C(Cl)N=C1 WCXDHFDTOYPNIE-RIYZIHGNSA-N 0.000 description 2
- IBSREHMXUMOFBB-JFUDTMANSA-N 5u8924t11h Chemical compound O1[C@@H](C)[C@H](O)[C@@H](OC)C[C@@H]1O[C@@H]1[C@@H](OC)C[C@H](O[C@@H]2C(=C/C[C@@H]3C[C@@H](C[C@@]4(O3)C=C[C@H](C)[C@@H](C(C)C)O4)OC(=O)[C@@H]3C=C(C)[C@@H](O)[C@H]4OC\C([C@@]34O)=C/C=C/[C@@H]2C)/C)O[C@H]1C.C1=C[C@H](C)[C@@H]([C@@H](C)CC)O[C@]11O[C@H](C\C=C(C)\[C@@H](O[C@@H]2O[C@@H](C)[C@H](O[C@@H]3O[C@@H](C)[C@H](O)[C@@H](OC)C3)[C@@H](OC)C2)[C@@H](C)\C=C\C=C/2[C@]3([C@H](C(=O)O4)C=C(C)[C@@H](O)[C@H]3OC\2)O)C[C@H]4C1 IBSREHMXUMOFBB-JFUDTMANSA-N 0.000 description 2
- 239000005660 Abamectin Substances 0.000 description 2
- 239000005875 Acetamiprid Substances 0.000 description 2
- 241000245654 Gladiolus Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 229950008167 abamectin Drugs 0.000 description 2
- 239000003096 antiparasitic agent Substances 0.000 description 2
- 210000000081 body of the sternum Anatomy 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000000857 drug effect Effects 0.000 description 2
- 208000010824 fish disease Diseases 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 210000003128 head Anatomy 0.000 description 2
- 230000000749 insecticidal effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- MGSRCZKZVOBKFT-UHFFFAOYSA-N thymol Chemical compound CC(C)C1=CC=C(C)C=C1O MGSRCZKZVOBKFT-UHFFFAOYSA-N 0.000 description 2
- 241000972773 Aulopiformes Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 206010006895 Cachexia Diseases 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 241000252211 Carassius Species 0.000 description 1
- 241000252229 Carassius auratus Species 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 239000005944 Chlorpyrifos Substances 0.000 description 1
- 241000223782 Ciliophora Species 0.000 description 1
- 241000239250 Copepoda Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000252228 Ctenopharyngodon Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 241000696958 Lernaea Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241001674048 Phthiraptera Species 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 239000005844 Thymol Substances 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 239000000921 anthelmintic agent Substances 0.000 description 1
- 229940124339 anthelmintic agent Drugs 0.000 description 1
- 229940125687 antiparasitic agent Drugs 0.000 description 1
- 239000003904 antiprotozoal agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- SBPBAQFWLVIOKP-UHFFFAOYSA-N chlorpyrifos Chemical compound CCOP(=S)(OCC)OC1=NC(Cl)=C(Cl)C=C1Cl SBPBAQFWLVIOKP-UHFFFAOYSA-N 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 244000078703 ectoparasite Species 0.000 description 1
- 208000026500 emaciation Diseases 0.000 description 1
- 239000004495 emulsifiable concentrate Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 210000001508 eye Anatomy 0.000 description 1
- -1 fluorobenzoyl urea Chemical compound 0.000 description 1
- 210000002816 gill Anatomy 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 210000000214 mouth Anatomy 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 230000001338 necrotic effect Effects 0.000 description 1
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000003250 oocyst Anatomy 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229960000790 thymol Drugs 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the field of aquaculture, and in particular relates to an aquatic insecticide containing hexaflumuron and application thereof, wherein the aquatic insecticide contains an active ingredient and a synergist; the active ingredient is hexaflumuron, and the synergist is carvacrol; the mass ratio of the active ingredients to the synergist is 5-25:1-5. The synergistic agent carvacrol is added into the hexaflumuron, so that the control effect of the hexaflumuron on the hawk can be obviously improved; especially when the mass ratio of the hexaflumuron to the carvacrol is 10:1, the control effect is further enhanced. The preparation is safe to fish and has no phytotoxicity.
Description
Technical Field
The invention belongs to the field of aquaculture, and particularly relates to an aquatic insecticide containing hexaflumuron and application thereof.
Background
With the rapid development of intensive aquaculture industry, fish diseases caused by parasites frequently outbreak, and the fish diseases cause great harm to fishery production. Parasitic diseases of fish not only affect the growth and reproduction of fish, resulting in emaciation, reduced immunity and poor disease resistance, but also affect the quality of fish. Therefore, the fish parasitic diseases cannot be ignored, and the killing and the control are carried out periodically according to the severity of the fish parasitic diseases and the parasite reproduction period. Most of the control of parasitic diseases in fish is currently using chemical pesticides. The fish antiparasitic agents are mainly classified into antiprotozoal agents (including repelling and killing sporozoons, ciliates, asparaffins, etc.), anthelmintic agents (including repelling and killing monofansis, parafansis, cestodes, etc.), and parasitic crustacean agents (including repelling and killing copepods, etc.).
The naea belongs to the family of the gladiolus, the order of the gladiolus, and the family of the naea, and is a crustacean parasitized at gills, skins, fins, eyes, heads, oral cavities and the like of fishes. The head of the hawk is drilled into the fish body to absorb nutrition, so that the fish body is lean. The part of the fish body drilled in by the naea is broken, the skin and muscle tissues are inflamed and red, the tissues are necrotic, and the hydromycete invades the cluster. The part of the hawk exposed outside the body surface of the fish is often parasitized by clockworm and algae, and looks like a bundle of gray cotton wool. When the fish body is infected with the solonergea in a large amount, the fish body is covered with the Chinese character 'the auricle' so called as 'the auricle disease'. In recent years, with the increase of medicine for the naea, the phenomenon that the naea is killed and easy to relapse occurs in many areas, and the naea becomes a difficult problem in the process of raising fishes for farmers.
The life history of the hawk is divided into nauplii, copepodi and adults. Nauplii of the hawk are developed into radial foot larvae after 5 times of molting, and the radial foot larvae are parasitic to fish bodies through mating after 4 times of molting. When the ambient water temperature is 18-20 ℃, 5-6 days are required from oocyst incubation to 1 st radial foot larva, and when the water temperature is about 25 ℃,3 days are required. The 1 st radial foot larva grows into a 5 th radial foot larva, and the water temperature is 16-20 ℃, and the 5-8 days are needed for the hawk of the parasitic grass carp. The adult stage of the haonea can be divided into 3 stages of 'child insects', 'strong insects', 'old insects' according to the form of the insect body. Once the hawk is parasitic on the fish body, the insect body in the stage of ' child's insect ', ' strong insect ' or ' old insect ' is difficult to be acted on by the drug because the body part possibly affected by the drug is deeply invaded into the muscle of the fish body.
In recent years, benzoylurea insecticides have been used in both livestock and aquaculture, where diflubenzuron, novaluron, flubenuron and insecticidal urea were used initially for plant protection and later for veterinary use, but chlorpyrifos was used only for veterinary use. Approved uses for benzoylurea insecticides include the treatment of animals (e.g., cattle, sheep, pigs, chickens), horses, cats, dogs, etc., producing foods of animal origin. At present, the benzoylurea insecticides used in the aquaculture field are mainly diflubenzuron and fluorobenzoyl urea, which are commonly found in the pre-stages of ectoparasite larvae and adults in the treatment of salmon lice. In aquaculture, diflubenzuron and flubenuron pesticides can be transmitted through the host's mucus, skin and blood and act by interfering with the synthesis of chitin in the exoskeleton. In recent years, some scholars at home and abroad have conducted a certain research on the application of benzoyl urea pesticides in aquaculture, and mainly comprise the usage amount, residual metabolism condition, toxicity research and the influence of the benzoyl urea pesticides on the environment. Along with the wider and wider application, the residual problem of the pesticide gradually appears, and threatens the diet health of consumers, so that the scientific and reasonable selection of antiparasitic drugs is particularly important, the research and development of the insecticidal composition capable of improving the efficacy can obviously reduce the dosage of the pesticide, reduce the environmental pollution, reduce the residual and ensure the food safety. CN107714712a discloses a hexaflumuron composition for preventing and treating the naea disease, which comprises an active component and an auxiliary material component, wherein the active component comprises hexaflumuron, acetamiprid and abamectin; the weight ratio of the hexaflumuron to the acetamiprid to the abamectin is 1: (0.01-0.2): (0.1-20). There is no report in the prior art about synergists for controlling the aquatic parasite hexaflumuron.
Disclosure of Invention
The invention aims to provide
An aquatic insecticide containing hexaflumuron comprises an active ingredient and a synergist; the active ingredient is hexaflumuron, and the synergist is carvacrol; the mass ratio of the active ingredients to the synergist is 5-25:1-5. Preferably, the mass ratio of the active ingredients to the synergistic agent is 5-20:1-4. Preferably, the mass ratio of the active ingredients to the synergistic agent is 10-20:1-2.
The aquatic insecticide also comprises auxiliary materials, wherein the weight percentage of the active ingredients is 5-25%.
The pesticide is prepared from emulsifiable concentrate, aqueous emulsion and microemulsion.
The aquatic pesticide is used for preventing and controlling parasites on aquatic products, wherein the parasites are fish crustacean parasites. Preferably, the parasite is Levonaea.
The application of carvacrol in enhancing the control effect of hexaflumuron on the naea is provided.
By adopting the technical scheme, the invention has the following beneficial effects:
The synergistic agent carvacrol is added into the hexaflumuron, so that the control effect of the hexaflumuron on the hawk can be obviously improved; especially when the mass ratio of the hexaflumuron to the carvacrol is 10:1, the control effect is further enhanced. The preparation is safe to fish and has no phytotoxicity.
Detailed Description
All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention is further illustrated with reference to specific embodiments.
Example 1: indoor efficacy evaluation of aquatic pesticide on grass carp Leptonaea
Test object: grass carp with Levonaea is taken from a fish pond with disease;
testing the medicament:
medicament 1:5% hexaflumuron preparation
Comprises the following components in percentage by weight: 5% of hexaflumuron, 4% of ethanol, 6% of pesticide emulsion 500#6%, 3% of Tween 80 and 100% of methyl oleate.
Medicament 2:5% hexaflumuron preparation
Comprises the following components in percentage by weight: 5% of hexaflumuron, 1% of carvacrol, 4% of ethanol, 6% of agricultural emulsion 500#6%, 3% of Tween 80 and 100% of methyl oleate.
Medicament 3:5% hexaflumuron preparation
Comprises the following components in percentage by weight: 5% of hexaflumuron, 1% of thymol, 4% of ethanol, 6% of pesticide emulsion 500#80, 3% of tween 80 and 100% of methyl oleate.
Agent 4:10% hexaflumuron preparation
Comprises the following components in percentage by weight: 10% of hexaflumuron, 1% of carvacrol, 4% of ethanol, 5% of agricultural emulsion 500#5%, 7% of Tween 80 and 100% of methyl oleate.
Medicament 5:15% hexaflumuron preparation
Comprises the following components in percentage by weight: 15% of hexaflumuron, 1% of carvacrol, 4% of ethanol, 5% of agricultural emulsion 500#5%, 7% of Tween 80 and 100% of methyl oleate.
Agent 6:20% hexaflumuron preparation
Comprises the following components in percentage by weight: 20% of hexaflumuron, 1% of carvacrol, 4% of ethanol, 5% of agricultural emulsion 500#5%, 7% of Tween 80 and 100% of methyl oleate.
The testing method comprises the following steps:
The test water comes from a fish pond with disease, the test container is a plastic water tank, each water tank stores 200L of pond water, the water temperature (27+/-0.5) DEG C, the pH value is 7.4, and the test period is continuously inflated. The doses of the agents administered are shown in the following table. 10 diseased grass carps are put in each water tank, the number of hausens attached to each tested fish is counted before putting, the number of residual insects of each test group hausens for 72 hours is recorded, and correction and prevention effects are calculated; the test was repeated 4 times and a blank control was set.
Correction control (%) = (control zone survival-control zone survival)/control zone survival x 100.
Test results:
As shown in Table 1, when the test concentration of hexaflumuron in the medicament 1 is 0.8mg/L, the control effect on the grass carp hawk is 72.6%; the synergistic agent carvacrol is added in the medicament 2, so that the control effect of the hexaflumuron on the hawk of grass carp can be obviously improved; particularly, when the mass ratio of the hexaflumuron to the carvacrol in the medicament 4 is 10:1, the control effect is further enhanced. In addition, the carvacrol alone has no control effect on the solenonaea of crucian. The preparation is safe to grass carp and has no phytotoxicity.
Table 1: indoor efficacy evaluation of aquatic pesticide on grass carp Leptonaea
Example 2: indoor drug effect evaluation of aquatic pesticide on crucian hausea
Test object: the crucian with the lernaea is taken from a fish pond with onset;
testing the medicament: agents 1-6 of example 1;
The test water comes from a fish pond with disease, the test container is a plastic water tank, each water tank stores 200L of pool water, and the test period is continuously inflated. The doses of the agents administered are shown in the following table. 10 diseased crucian carps are put in each water tank, the number of the hausens attached to each tested fish is counted before putting, the number of residual insects of the hausens of each test group for 72 hours is recorded, and correction and prevention effects are calculated; the test was repeated 4 times and a blank control was set.
Correction control (%) = (control zone survival-control zone survival)/control zone survival x 100.
Test results:
As shown in Table 2, when the test concentration of hexaflumuron in the medicament 1 is 0.5mg/L, the control effect on the Carassius auratus is 70.3%; the synergistic agent carvacrol is added in the medicament 2, so that the control effect of the hexaflumuron on the solenonaea of the crucian can be obviously improved; particularly, when the mass ratio of the hexaflumuron to the carvacrol in the medicament 4 is 10:1, the control effect is further enhanced. In addition, the carvacrol alone has no control effect on the solenonaea of crucian. The preparation is safe to crucian and has no drug damage.
Table 2: indoor drug effect evaluation of aquatic pesticide on crucian hausea
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same.
Claims (8)
1. An aquatic insecticide containing hexaflumuron is characterized by comprising an active ingredient and a synergist; the active ingredient is hexaflumuron, and the synergist is carvacrol; the mass ratio of the active ingredients to the synergist is 5-25:1-5.
2. The aquatic product pesticide according to claim 1, wherein the mass ratio of the active ingredient to the synergist is 5-20:1-4.
3. The aquatic product pesticide according to claim 2, wherein the mass ratio of the active ingredient to the synergist is 10-20:1-2.
4. An aquatic product pesticide according to any one of claims 1 to 3, further comprising an adjuvant component in an amount of 5 to 25% by weight of the active ingredient.
5. The aquatic product pesticide of claim 4 wherein the pesticide is formulated as a cream, an aqueous emulsion, or a microemulsion.
6. Use of an aquatic insecticide according to any one of claims 1 to 5 for parasite control on aquatic products.
7. The use according to claim 6, wherein the parasite is a fish crustacean parasite.
8. The use according to claim 7, wherein the parasite is haemala.
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