CN117919215A - Aquatic insecticide containing lufenuron and application thereof - Google Patents

Abstract

The invention belongs to the field of aquaculture, and in particular relates to an aquatic insecticide containing lufenuron and application thereof, wherein the aquatic insecticide contains an active ingredient and a synergistic agent; the active ingredient is lufenuron, and the synergist is carvacrol; the mass ratio of the active ingredients to the synergist is 5-30:1-5. The synergistic agent carvacrol is added into the lufenuron, so that the control effect of the lufenuron on the hawk can be obviously improved; especially when the mass ratio of lufenuron to carvacrol is 5-15:1, the control effect is further enhanced. The preparation is safe to fish and has no phytotoxicity.

Description

Aquatic insecticide containing lufenuron and application thereof

Technical Field

The invention belongs to the field of aquaculture, and particularly relates to an aquatic insecticide containing lufenuron and application thereof.

Background

With the rapid development of intensive aquaculture industry, fish diseases caused by parasites frequently outbreak, and the fish diseases cause great harm to fishery production. Parasitic diseases of fish not only affect the growth and reproduction of fish, resulting in emaciation, reduced immunity and poor disease resistance, but also affect the quality of fish. Therefore, the fish parasitic diseases cannot be ignored, and the killing and the control are carried out periodically according to the severity of the fish parasitic diseases and the parasite reproduction period. Most of the control of parasitic diseases in fish is currently using chemical pesticides. The fish antiparasitic agents are mainly classified into antiprotozoal agents (including repelling and killing sporozoons, ciliates, asparaffins, etc.), anthelmintic agents (including repelling and killing monofansis, parafansis, cestodes, etc.), and parasitic crustacean agents (including repelling and killing copepods, etc.).

The naea belongs to the family of the gladiolus, the order of the gladiolus, and the family of the naea, and is a crustacean parasitized at gills, skins, fins, eyes, heads, oral cavities and the like of fishes. The head of the hawk is drilled into the fish body to absorb nutrition, so that the fish body is lean. The part of the fish body drilled in by the naea is broken, the skin and muscle tissues are inflamed and red, the tissues are necrotic, and the hydromycete invades the cluster. The part of the hawk exposed outside the body surface of the fish is often parasitized by clockworm and algae, and looks like a bundle of gray cotton wool. When the fish body is infected with the solonergea in a large amount, the fish body is covered with the Chinese character 'the auricle' so called as 'the auricle disease'. In recent years, with the increase of medicine for the naea, the phenomenon that the naea is killed and easy to relapse occurs in many areas, and the naea becomes a difficult problem in the process of raising fishes for farmers.

The life history of the hawk is divided into nauplii, copepodi and adults. Nauplii of the hawk are developed into radial foot larvae after 5 times of molting, and the radial foot larvae are parasitic to fish bodies through mating after 4 times of molting. When the ambient water temperature is 18-20 ℃, 5-6 days are required from oocyst incubation to 1 st radial foot larva, and when the water temperature is about 25 ℃,3 days are required. The 1 st radial foot larva grows into a 5 th radial foot larva, and the water temperature is 16-20 ℃, and the 5-8 days are needed for the hawk of the parasitic grass carp. The adult stage of the haonea can be divided into 3 stages of 'child insects', 'strong insects', 'old insects' according to the form of the insect body. Once the hawk is parasitic on the fish body, the insect body in the stage of ' child's insect ', ' strong insect ' or ' old insect ' is difficult to be acted on by the drug because the body part possibly affected by the drug is deeply invaded into the muscle of the fish body.

In recent years, benzoylurea insecticides have been used in both livestock and aquaculture, where diflubenzuron, novaluron, flubenuron and insecticidal urea were used initially for plant protection and later for veterinary use, but chlorpyrifos was used only for veterinary use. Approved uses for benzoylurea insecticides include the treatment of animals (e.g., cattle, sheep, pigs, chickens), horses, cats, dogs, etc., producing foods of animal origin. At present, the benzoylurea insecticides used in the aquaculture field are mainly diflubenzuron and fluorobenzoyl urea, which are commonly found in the pre-stages of ectoparasite larvae and adults in the treatment of salmon lice. In aquaculture, diflubenzuron and flubenuron pesticides can be transmitted through the host's mucus, skin and blood and act by interfering with the synthesis of chitin in the exoskeleton. In recent years, some scholars at home and abroad have conducted a certain research on the application of benzoyl urea pesticides in aquaculture, and mainly comprise the usage amount, residual metabolism condition, toxicity research and the influence of the benzoyl urea pesticides on the environment. Along with the wider and wider application, the residual problem of the pesticide gradually appears, and threatens the diet health of consumers, so that the scientific and reasonable selection of antiparasitic drugs is particularly important, the research and development of the insecticidal composition capable of improving the efficacy can obviously reduce the dosage of the pesticide, reduce the environmental pollution, reduce the residual and ensure the food safety. CN107714712a discloses a hexaflumuron composition for preventing and treating the naea disease, which comprises an active component and an auxiliary material component, wherein the active component comprises hexaflumuron, acetamiprid and abamectin; the weight ratio of the hexaflumuron to the acetamiprid to the abamectin is 1: (0.01-0.2): (0.1-20). There is no report in the prior art about synergists for controlling the aquatic parasite hexaflumuron.

Disclosure of Invention

The invention aims to provide an aquatic insecticide containing lufenuron, which comprises an active ingredient and a synergist; the active ingredient is lufenuron, and the synergist is carvacrol; the mass ratio of the active ingredients to the synergist is 5-30:1-5. Preferably, the mass ratio of the active ingredients to the synergistic agent is 5-20:1-4. Preferably, the mass ratio of the active ingredients to the synergistic agent is 5-15:1.

The aquatic insecticide also comprises auxiliary materials, wherein the weight percentage of the active ingredients is 5-25%.

The pesticide is prepared from emulsifiable concentrate, aqueous emulsion and microemulsion.

The aquatic pesticide is used for preventing and controlling parasites on aquatic products, wherein the parasites are fish crustacean parasites. Preferably, the parasite is Levonaea.

Use of carvacrol for enhancing the control effect of lufenuron on the naea.

By adopting the technical scheme, the invention has the following beneficial effects:

The synergistic agent carvacrol is added into the lufenuron, so that the control effect of the lufenuron on the hawk can be obviously improved; especially when the mass ratio of lufenuron to carvacrol is 5-15:1, the control effect is further enhanced. The preparation is safe to fish and has no phytotoxicity.

Detailed Description

All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

The invention is further illustrated with reference to specific embodiments.

Example 1: indoor efficacy evaluation of aquatic pesticide on grass carp Leptonaea

Test object: grass carp with Levonaea is taken from a fish pond with disease;

Testing the medicament:

medicament 1:5% lufenuron preparation

Comprises the following components in percentage by weight: 5% of lufenuron, 3% of ethanol, 500#4% of agro-emulsion, 4% of Tween-80 and 100% of methyl oleate.

Medicament 2:5% lufenuron preparation

Comprises the following components in percentage by weight: 5% of lufenuron, 1% of carvacrol, 3% of ethanol, 4% of agricultural emulsion 500#4%, 4% of Tween 80 and 100% of methyl oleate.

Medicament 3:5% lufenuron preparation

Comprises the following components in percentage by weight: 5% of lufenuron, 1% of thymol, 3% of ethanol, 4% of pesticide emulsion 500#4%, 4% of tween 80 and 100% of methyl oleate.

Agent 4:10% lufenuron preparation

Comprises the following components in percentage by weight: 10% of lufenuron, 1% of carvacrol, 3% of ethanol, 5% of agricultural emulsion 500#5%, 5% of Tween 80 and 100% of methyl oleate.

Agent 5:15% lufenuron preparation

Comprises the following components in percentage by weight: 15% of lufenuron, 1% of carvacrol, 3% of ethanol, 5% of agricultural emulsion 500#5%, 5% of tween 80 and 100% of methyl oleate.

Agent 6:20% lufenuron preparation

Comprises the following components in percentage by weight: 20% of lufenuron, 1% of carvacrol, 3% of ethanol, 5% of agricultural emulsion 500#5%, 5% of Tween 80 and 100% of methyl oleate.

The testing method comprises the following steps:

the test water comes from a fish pond with disease, the test container is a plastic water tank, each water tank stores 200L of pond water, the water temperature (27+/-0.5) DEG C, the pH value is 7.4, and the test period is continuously inflated. The dosages of the agents are shown in the table below. 10 diseased grass carps are put in each water tank, the number of hausens attached to each tested fish is counted before putting, the number of residual insects of each test group hausens for 72 hours is recorded, and correction and prevention effects are calculated; the test was repeated 4 times and a blank control was set.

Correction control (%) = (control zone survival-control zone survival)/control zone survival x 100.

Test results:

As can be seen from Table 1, the test concentration of lufenuron in the medicament 1 is 1mg/L, the control effect on grass carp fluke is 79.3%; the synergistic agent carvacrol is added in the medicament 2, so that the control effect of lufenuron on hausena of grass carp can be obviously improved; in particular, when the mass ratio of lufenuron to carvacrol in the medicaments 2,4 and 5 is 5-15:1, the control effect is further enhanced. Thymol in the medicament 3 has no synergistic effect. In addition, the carvacrol alone has no control effect on the grass carp naea. The preparation is safe to grass carp and has no phytotoxicity.

Table 1: indoor efficacy evaluation of aquatic pesticide on grass carp Leptonaea

Example 2: indoor drug effect evaluation of aquatic pesticide on crucian hausea

Test object: the crucian with the lernaea is taken from a fish pond with onset;

Testing the medicament: agents 1-6 of example 1;

The test water comes from a fish pond with disease, the test container is a plastic water tank, each water tank stores 200L of pool water, and the test period is continuously inflated. The dosages of the agents are shown in the table below. 10 diseased crucian carps are put in each water tank, the number of the hausens attached to each tested fish is counted before putting, the number of residual insects of the hausens of each test group for 72 hours is recorded, and correction and prevention effects are calculated; the test was repeated 4 times and a blank control was set.

Correction control (%) = (control zone survival-control zone survival)/control zone survival x 100.

Test results:

As shown in Table 2, when the test concentration of lufenuron in the medicament 1 is 0.8mg/L, the control effect on the Lenaea of crucian is 75.2%; the synergistic agent carvacrol is added in the medicament 2, so that the control effect of lufenuron on the solenonaea of crucian can be obviously improved; in particular, when the mass ratio of lufenuron to carvacrol in the medicaments 2, 4 and 5 is 5-15:1, the control effect is further enhanced. Thymol in the medicament 3 has no synergistic effect. In addition, the carvacrol alone has no control effect on the solenonaea of crucian. The preparation is safe to crucian and has no drug damage.

Table 2: indoor drug effect evaluation of aquatic pesticide on crucian hausea

Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same.

Claims (8)

1. An aquatic insecticide containing lufenuron, which is characterized by comprising an active ingredient and a synergistic agent; the active ingredient is lufenuron, and the synergist is carvacrol; the mass ratio of the active ingredients to the synergist is 5-30:1-5.

2. The aquatic product pesticide according to claim 1, wherein the mass ratio of the active ingredient to the synergist is 5-20:1-4.

3. The aquatic product pesticide according to claim 2, wherein the mass ratio of the active ingredient to the synergist is 5-15:1.

4. An aquatic product pesticide according to any one of claims 1 to 3, further comprising an adjuvant component in an amount of 5 to 25% by weight of the active ingredient.

5. The aquatic product pesticide of claim 4 wherein the pesticide is formulated as a cream, an aqueous emulsion, or a microemulsion.

6. Use of an aquatic insecticide according to any one of claims 1 to 5 for parasite control on aquatic products.

7. The use according to claim 6, wherein the parasite is a fish crustacean parasite.

8. The use according to claim 7, wherein the parasite is haemala.