CN118203005A - Botanical attractant for soybean thrips and application thereof - Google Patents
Botanical attractant for soybean thrips and application thereof Download PDFInfo
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- CN118203005A CN118203005A CN202410619634.9A CN202410619634A CN118203005A CN 118203005 A CN118203005 A CN 118203005A CN 202410619634 A CN202410619634 A CN 202410619634A CN 118203005 A CN118203005 A CN 118203005A
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- thrips
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Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a plant source attractant for soybean thrips and application thereof. The 2, 4-decadienal with obvious attracting effect on the soybean thrips is identified from the flower separation of the soybean thrips non-host plant, namely the wood milk fruit, and can be used for monitoring and preventing the soybean thrips, and compared with a plant source attractant screened from cowpea, the plant source attractant is not diluted and covered by the smell of cowpea after application; compared with the existing color plate trapping technology, the 2, 4-decadienal can trap and kill soybean thrips all-weather. The 2, 4-decadienal is derived from plants, is environment-friendly, and has high application value.
Description
Technical Field
The invention belongs to the technical field of green prevention and control of pests, and particularly relates to a plant source attractant for soybean thrips and application thereof.
Background
Thrips sojae (Megalurothrips usitatus (Bagnall)) is a multi-feeding pest that can be harmful to 28 crops such as cowpea, peanut, soybean, etc., wherein the damage to cowpea is the most serious. The bean thrips adults and nymphs prefer to file and absorb the juice of cowpea buds, leaves, flowers, pods and other organs, which often results in growth stagnation, malformation, flower and pod falling, or form flaky black or brown teeth on fruits, so that the quality and quality of cowpeas are seriously affected. In view of the serious hazard of the soybean thrips, the soybean thrips are listed in a 'first crop disease and pest damage directory' for key management in 2023, 3 and 7 days in China.
The physical and chemical attraction control technology is an important technical measure for preventing and controlling soybean thrips, and is mainly realized by suspending color plates and information compounds with attraction effect in the field. Tang Liangde et al found that blue plates of soybean thrips preferences 440 nm and 461 nm (plant protection, 2015, 41 (6): 169-172), liyang et al identified screening 4 volatile substances of beta-caryophyllene, phytol, ethyl palmitate, o-xylene, etc. from soybean thrips host plants had significant attraction to soybean thrips (environmental insect theory report, 2021, 43 (6): 1566-1580), however, the trapping distance of blue plates was limited, volatiles screened from host plants were easily disturbed by volatiles released from host plants in application, and compounds screened from host plants were lacking field test verification. Dan Wangpeng and other scholars separate and identify geranyl acetate and farnesyl acetate from male thrips sojae as aggregation pheromone of the soybean thrips, (CN 201810865181.2), while Li Xiaowei and other scholars consider that farnesyl acetate is aggregation pheromone of the soybean thrips (insect school report, 2019, 62 (9): 1017-1027), and the components and effects of the aggregation pheromone still need to be further verified. Based on the background, diversified and novel physicochemical induction control products and technologies are yet to be developed.
Disclosure of Invention
The invention aims to provide a plant source attractant for soybean thrips and application thereof, aiming at the problems, although the current soybean thrips outbreak into disaster in southern areas of China, a physical and chemical control technology is an important measure for preventing and controlling the thrips, and active substance types for efficiently attracting the soybean thrips are less.
In the early stage, the inventors found a large number of adult soybean thrips in flowers of the soybean thrips non-host plant, wood milk fruit (Baccaurea ramifloralour.), which suggests that volatile substances with strong attractant activity for soybean thrips are present in the wood milk fruit flowers. Based on the method, the volatile matters with the attraction effect on the soybean thrips are separated, identified and screened from the wood milk fruit flowers, so that the attractant is prepared, and important foundation and technical support are provided for monitoring and trapping the soybean thrips. According to the invention, volatile substances of the Muzhui flowers are collected and identified through a dynamic headspace adsorption method and a gas chromatography mass spectrometry (GC-MS), an EAG (insect antenna potential), a Y-type olfactometer and the like are adopted to perform a behavior selection test, and a field trapping test is combined to screen and verify that 2, 4-decadienal (chemical formula: C 10H16 O) which is a volatile substance with remarkable trapping effect on soybean thrips is obtained.
It is a first object of the present invention to provide the use of 2, 4-decadienal for controlling the plant thistle.
Preferably, the 2, 4-decadienal is used as an active attracting component for attracting the soybean thrips.
The second object of the invention is to provide the application of the plant source attractant in preventing and controlling the field thistle, which is characterized in that the attractant contains 2, 4-decadienal as an attracting active ingredient, and the concentration of the 2, 4-decadienal in the attractant is 0.1 mu g/mu L-100 mu g/mu L.
Preferably, the concentration of 2, 4-decadienal in the attractant is 0.1 μg/μl to 1 μg/μl.
Preferably, the concentration of 2, 4-decadienal in the attractant is 0.1 μg/μl.
Preferably, the solvent of the attractant is n-hexane or paraffin oil.
Preferably, the soybean thrips is female and male soybean thrips.
The third object of the invention is to provide a method for preventing and controlling soybean thrips, which comprises the following steps: placing 2, 4-decadienal, the attractant or the trap with the attractant in a target area of soybean thrips to be trapped.
Preferably, the trap is an adhesive trap.
The invention has the beneficial effects that:
1. according to the invention, the volatile matter with the attracting effect on the soybean thrips is separated, identified and screened from the wood milk fruit flowers, wherein the 2, 4-decadienal with the best attracting effect is prepared into the attractant, and an important basis and technical support can be provided for monitoring and preventing and controlling the soybean thrips.
2. The volatile matter for trapping and killing the soybean thrips is separated, identified and screened from the fruit flowers of the mare milk, can be used for monitoring and preventing and controlling the soybean thrips, and is not diluted and covered by the smell of the cowpea after being applied compared with the plant source attractant screened from the cowpea; compared with the existing color plate trapping technology, the 2, 4-decadienal can trap and kill soybean thrips all-weather.
3. The 2, 4-decadienal used in the invention is derived from plants, is environment-friendly, and has a relatively high application value.
Drawings
FIG. 1 is an olfactory selection of thrips sojae atricolor on Mukouossi flower, cowpea flower; and (3) injection: chi-square test indicates that the attraction of the Mukouossi flower and cowpea flower to soybean thrips is significantly different from that of the control (air) at the level of P <0.001 respectively.
FIG. 2 is a total ion mass spectrum of the Mumilk fruit flower volatiles; wherein "1" represents ocimene, "2" represents linalool, "3" represents trans-2-decenal, "4" represents 2, 4-decadienal, and "5" represents trans-2-undecenal.
FIG. 3 is a total ion chromatogram comparison of volatiles isolated from Mucuna flowers with their compound standards; and (3) injection: a is the total ion chromatogram of the wood milk fruit flower volatiles; b is the total ion chromatogram of the test compound standard; "1" represents ocimene, "2" represents linalool, "3" represents trans-2-decenal, "4" represents 2, 4-decadienal, and "5" represents trans-2-undecenal.
FIG. 4 is an electrophysiological response of different volatiles to soybean thrips; and (3) injection: a is the relative reaction value of EAG of linalool with different concentrations, B is the relative reaction value of EAG of 2, 4-decadienal with different concentrations, C is the relative reaction value of EAG of trans-2-undecylenal with different concentrations, D is the relative reaction value of EAG of trans-2-decenal with different concentrations, E is the relative reaction value of EAG of ocimene with different concentrations; different lowercase letters indicate that there was a significant difference (P < 0.05) between different concentrations of the same volatiles as analyzed by Duncan.
FIG. 5 is a selection of different volatiles for soybean thrips; and (3) injection: a is the selectivity of the soybean thrips to linalool with different concentrations, B is the selectivity of the soybean thrips to 2, 4-decadienal with different concentrations, C is the selectivity of the soybean thrips to trans-2-undecylenic aldehyde with different concentrations, D is the selectivity of the soybean thrips to trans-2-decenal with different concentrations, and E is the selectivity of the soybean thrips to ocimene with different concentrations; * The terms "a", "b" and "c" refer to the number of thrips sojae atricolor that was induced at the same concentration of the same volatiles and the number of thrips sojae atricolor induced by the control (n-hexane) as measured by Kruskal-Wallis test, respectively, at levels P <0.05, P <0.01 and P <0.001, respectively.
FIG. 6 is a comparison of the attraction of different concentrations of the same volatiles to soybean thrips.
Fig. 7 is a comparison of the attraction of different volatile optimum attraction concentrations to soybean thrips.
FIG. 8 is a comparison of the trapping effect of different concentrations of volatiles on a field of thrips sojae; and (3) injection: values in the figures are mean ± standard deviation (n=10), different letters indicate significant differences between different concentrations (P < 0.05) by Duncan analysis; the first data for each volatiles was control, and the concentrations of 2, 4-decadienal were paraffin oil, 0.01. Mu.g/. Mu.L, 0.05. Mu.g/. Mu.L, 0.1. Mu.g/. Mu.L, 0.5. Mu.g/. Mu.L, and 1. Mu.g/. Mu.L in order from left to right, linalool, trans-2-decenal, trans-2-undecylaldehyde were paraffin oil, 1. Mu.g/. Mu.L, 5. Mu.g/. Mu.L, 10. Mu.g/. Mu.L, 50. Mu.g/. Mu.L, and 100. Mu.g/. Mu.L in order from left to right.
Detailed Description
The following examples are further illustrative of the invention and are not intended to be limiting thereof.
The number of soybean thrips male insects is very small, and the reproduction mode of soybean thrips is mainly parthenogenetic oviposition, so that the soybean thrips female insects can be induced and collected to be the most critical for preventing and controlling soybean thrips, and therefore, the indoor experiments in the following embodiments are carried out by using soybean thrips female adults.
Example 1
1. Trending behavior of female adult soybean thrips on Mumeria nivea flowers and cowpea flowers
And testing the trend behaviors of female adults of the soybean thrips on the wood milk flowers and the cowpea flowers by adopting a Y-shaped olfactory instrument.
The Y-type olfactory instrument is formed by connecting an air pump, a drying tower, a gas washing bottle, a flavor source bottle, a flowmeter and a Y-type glass tube in sequence through a Teflon tube. The air flow rate of the two arms is controlled at 200 mL/min by a gas flowmeter. The drying tower is provided with active carbon for purifying air, and the gas washing cylinder is provided with 2/3 distilled water for humidifying. All glassware was washed with absolute ethanol and then distilled water. In order to avoid interference of uneven environmental light intensity on the selection behavior of thrips, a piece of black cloth is used for covering the whole Y-shaped glass tube, so that the light intensity of the two test arms is the same. The experiment was performed at room temperature of 26℃and a relative humidity of 70% -75%, and the experiment was performed by selecting the time (9:00 to 14:00) for which insects were active.
Fresh cowpea flowers and wood milk fruit flowers are respectively placed in taste source bottles, and air is used as a blank control. One bean thrips female adult is selected each time and placed in a Y-shaped glass tube for experiment, observation is carried out within 5 minutes after each experiment is started, and when thrips enter 2/3 of the side wall within 5 minutes and stay for more than 30 seconds, the animal is marked as having a reaction to substances in the side wall. Behavior observations were terminated when no selection was made after thrips entered the side wall for 5 minutes. Each treatment tested 100 bean thrips female adults. In order to eliminate the influence of the pipe wall position effect, two side arm positions are exchanged for every 5 thrips to be tested, a clean Y-shaped glass pipe is replaced for every 10 thrips to be tested, the Teflon pipe, the gas washing cylinder and the Y-shaped glass pipe are cleaned by absolute ethyl alcohol after each test, and the glass pipe is used after being dried so as to eliminate the odor influence among different treatments.
The results of fig. 1 show that, compared with the control (air), both the marshmallow flowers and the cowpea flowers have a very significant attraction effect (P < 0.001) on female adults of soybean thrips; compared with cowpea flowers, the wood milk flowers attract more female imagoes of soybean thrips, but the difference is not obvious. This suggests that the roses of Mumeria nivea and cowpea have a comparable attraction to the female adults of Frankliniella douglas (P < 0.317).
2. Collecting and identifying volatile matters of Muzhi fruit flowers
The test is to collect and identify the volatile matters of the Mucuna flowers by a dynamic headspace adsorption method and a gas chromatography mass spectrometer (GC-MS instrument, purchased from Siemens, model: ISQ GC-MS). The wood-milk fruit flowers which are just collected are placed in a collecting bag, and the volatile matters of the wood-milk fruit flowers are collected by adopting a dynamic headspace absorption method under the indoor conditions that the temperature is 26 ℃ and the relative humidity is 60% -75%. The gas blown out from the vacuum pump is purified by a drying tower at the flow rate of 100 mL/min, then distilled water is distilled by a washing bottle, and then the distilled water is conveyed by a sampling bag, and finally enters a Tenax-TA sampling adsorption tube, and each time is collected for 6-10 hours. The harvested gas was then eluted 30 min with 2mL hexane and 30 min with 2mL ethane as a control. The solution was filtered through a 0.45 μm and 0.22 μm filter in sequence and then stored in a brown Agilent sample bottle. The vials were wrapped with tinfoil and stored in a-20 ℃ refrigerator.
Chromatographic conditions: siemens gas chromatograph-mass spectrometer ISQ GC-MS, TR-5 MS chromatographic column (30 m X250 μm), sample inlet temperature: nitrogen is adopted as carrier gas at the temperature of 250 ℃, the flow rate of the carrier gas is 1 mL/min, and the sample injection is not split in a 112 Kpa constant pressure mode. Mass spectrometry conditions: the ion source is EI 70 eV, the voltage is 350V, the interface temperature is 230 ℃, and the scanning voltage range is 33-400 m/Z. Heating program: the initial temperature was 50deg.C, 10 ℃/min to 180deg.C, holding 2min, and 5 ℃/min to 280℃and holding 5 min. 1 μl of each sample was sampled manually. And (3) calculating the content of each component of the obtained total ion chromatogram by using a peak area normalization method, and comparing with a NIST17 (National Institute of STANDARDS AND Technology) database to obtain the names, the peak time, the molecular formula, the CAS number and the relative content of various substances. The volatile matter collection and identification are repeated 5 times, the volatile matter with the highest occurrence frequency and the matching degree of more than 90% in the 5 times is determined as the volatile matter of the Mu-Nitraria fruit flowers, and the standard product is purchased for verification. Diluting the standard substance to 1 ppm by using normal hexane, taking 10 mu L of standard substance diluent by using a 50 mu L microsyringe, carrying out experiments according to the same GC-MS program, and then comparing and analyzing a mass spectrogram result of the standard sample with a mass spectrogram result of a volatile list to determine the types of the volatile substances.
60 Compounds are included in the Mucuna flowers volatiles, 17 volatiles remain after removing impurities and keeping substances with high matching degree (figure 2 and table 1), wherein ocimene, linalool, trans-2-decenal, 2, 4-decadienal and trans-2-undecylenic aldehyde exist stably in 5 repetitions, and the results are verified by the standard substances, so that the identification results are accurate (figure 3).
TABLE 1 identification of essential active ingredients of the volatile Wood, milk, fruit and flower
3. Electrophysiological response of female adult soybean thrips to volatile wood-milk-fruit flowers
Insect antennal potential (EAG) experiments, chemical standards were diluted with n-hexane and the dose-response chemical standard concentrations were 0.01. Mu.g/. Mu.L, 0.1. Mu.g/. Mu.L, 1. Mu.g/. Mu.L, 10. Mu.g/. Mu.L, and 100. Mu.g/. Mu.L. The reference and recording electrodes consisted of two glass capillaries (1.1 mm a diameter) filled with Ringer's electrolyte and two silver wires, each immersed in the glass capillaries. The head was cut at the groove between the head and the forebreast of the thistle and a small portion of the two forelegs and the two antenna ends was cut off. The head was placed in the reference electrode and the two antennas were placed in the recording electrode for testing.
Filter paper (3 mm ×40 mm) was placed in a 1mL pipette tip, and 15 μl of test compounds at concentrations of 0.01 μg/μl, 0.1 μg/μl, 1 μg/μl, 10 μg/μl, and 100 μg/μl were added, respectively, and the same volume of n-hexane treated filter paper (3 mm ×40 mm) was used as a control. The tip of the pipette tip was inserted into a stainless steel tube of diameter 12 mm and length 200 mm, the ends were connected by a silicone tube, and the air flow blown out of the stainless steel tube was about 5mm from the compound to be tested or n-hexane (control). Before each experiment, the pipette tip must be replaced with a new one. A strip of filter paper treated with n-hexane in the pipette tip was used as a control. N-hexane blank was tested once each before (EAG CK1) and after (EAG CK2) the chemical standard test. Stimulus (test compound or n-hexane (control)) was blown into the continuous humidified gas stream generated by the air stimulus controller for 0.5 seconds. Recording of the EAG signal starts 1 second before stimulation starts and lasts 10 seconds. Between each stimulus, the haptic (antenna) had a recovery time of at least 1 minute before testing. The absolute value of each compound reference is subtracted from the average of the two previous and subsequent controls to obtain the relative value of each compound reference. Each chemical standard was assayed 10 times in duplicate, 10 worms each time. The volatiles that trigger the antenna response of the adult thrips sojae were screened by comparing the electrophysiological response of the antenna pair volatiles to the control.
The results in FIG. 4 show that the adult soybean thrips has an electrophysiological response to all 5 volatiles, linalool (A in FIG. 4), 2, 4-decadienal (B in FIG. 4), trans-2-undecenal (C in FIG. 4), trans-2-decenal (D in FIG. 4) and ocimene (E in FIG. 4). Also, the EAG response of the female thrips sojae to these 5 volatiles increased with increasing concentration.
4. Selective behaviour of female adult thrips sojae atricolor on volatile wood-milk-fruit-flower
The test sets up the comparison of 5 different volatiles (linalool, 2, 4-decadienal, trans-2-undecylenic aldehyde, trans-2-decenal, ocimene) with a blank (air), the comparison between different concentrations of the same volatiles, the comparison between optimal concentrations of 5 different volatiles, and adopts a Y-type olfactometer test, and the experimental method is the same as the Y-type olfactometer test described above.
The female adult soybean thrips was behaving in response to all 5 volatiles. Wherein 1. Mu.g/. Mu.L, 10. Mu.g/. Mu.L and 100. Mu.g/. Mu.L linalool (A in FIG. 5), 0.1. Mu.g/. Mu.L, 1. Mu.g/. Mu.L, 10. Mu.g/. Mu.L and 100. Mu.g/. Mu.L 2, 4-decadienal (B in FIG. 5) and 10. Mu.g/. Mu.L and 100. Mu.g/. Mu.L of trans-2-undecylenic aldehyde (C in FIG. 5) all have a significant attracting effect on female adult soybean thrips; whereas 0.01 μg/μl, 0.1 μg/μl, 1 μg/μl and 10 μg/μl ocimene had a significant repellent effect on adult soybean thrips (E in fig. 5). 10. Mu g/. Mu.L of trans-2-decenal had a significant attracting effect on female adult soybean thrips, while 100. Mu.g/. Mu.L of trans-2-decenal had a significant repelling effect on adult soybean thrips (D in FIG. 5).
As only one concentration of the trans-2-decenal has an attracting effect on the female adult of the soybean thrips, the ocimene has a repellent effect on the female adult of the soybean thrips, and the attracting effects of linalool and 2, 4-decadienal on the female adult of the soybean thrips are further compared with the attracting effects of the same substance of the trans-2-undecylenic aldehyde on different concentrations of the female adult of the soybean thrips. According to the two conditions of cost saving and effect, there is no significant difference in the attraction effect on the soybean thrips female adults between 0.1 μg/μL, 1 μg/μL, 10 μg/μL and 100 μg/μL of 2, 4-decadienal, there is no significant difference in the attraction effect on the soybean thrips female adults between 10 μg/μL and 100 μg/μL of trans-2-undecylenic aldehyde, and 10 μg/μL of linalool is the best attraction effect on the soybean thrips female adults between 1 μg/μL, 10 μg/μL and 100 μg/μL of linalool, so that the best attraction concentration of 2, 4-decadienal on the soybean thrips female adults is 0.1 μg/μL, the best attraction concentration of trans-2-undecylenic aldehyde on the soybean thrips female adults is 10 μg/μL, and the best attraction effect of linalool on the soybean thrips female adults is 10 μg/μL, and the best attraction effect on the soybean thrips female adults is 10 μg/μL (the best attraction effect on the soybean thrips female adults is 6 μg/μL).
Further comparing the attraction effect between the optimal concentrations of linalool, 2, 4-decadienal, trans-2-undecylenic aldehyde, trans-2-decenal 4 substances with the attraction effect on the female adults of soybean thrips, the attraction effect of 0.1 μg/μl of 2, 4-decadienal on the female adults of soybean thrips was found to be strongest, followed by 10 μg/μl of linalool, and finally 10 μg/μl of trans-2-decenal and 10 μg/μl of trans-2-undecylenic aldehyde (fig. 7).
5. Four kinds of field attraction test of wood milk fruit volatile matters on soybean thrips
And selecting to perform a field attracting test in cowpea fields. According to the volatile matters which are screened out by the Y-shaped olfactometer and have an attracting effect on the soybean thrips indoors, the volatile matters are diluted by paraffin oil for standby with different concentration gradients. The concentrations of 2, 4-decadienal were set to 0.01. Mu.g/. Mu.L, 0.05. Mu.g/. Mu.L, 0.1. Mu.g/. Mu.L, 0.5. Mu.g/. Mu.L and 1. Mu.g/. Mu.L, linalool, trans-2-decenal, trans-2-undecyleal were all 1. Mu.g/. Mu.L, 5. Mu.g/. Mu.L, 10. Mu.g/. Mu.L, 50. Mu.g/. Mu.L and 100. Mu.g/. Mu.L. The adhesive trap, which is composed of a transparent plastic plate coated with viscose and a fiber rod fitted with a cross bar, is placed at a height of 1.2 meters from the ground. A high density sponge plate was attached to the center of the transparent plastic plate, and then 100. Mu.L of volatiles or a blank (paraffin oil) was drawn up on the sponge plate with a pipette and marked with a marker. The cowpea fields were about 60m a long each row, the adhesive traps were arranged in the center of each row, 10 adhesive traps were provided each row, each trap was spaced at least 5m a distance apart, at least one row (about 2m a) spaced apart between different concentrations, and each substance was repeated 10 times per concentration. The number of soybean thrips captured after 2 days was recorded.
As can be seen from FIG. 8, the 2, 4-decadienal attracted the best effect on the soybean thrips, and the adhesive trap captured not only the soybean thrips females but also the soybean thrips males, with the greatest attraction amounts of 0.1. Mu.g/. Mu.L, 0.5. Mu.g/. Mu.L and 1. Mu.g/. Mu.L, but the differences were not significant. Therefore, 2, 4-decadienal with the concentration of 0.1 mu g/mu L to 1 mu g/mu L is recommended to attract soybean thrips.
Claims (9)
- Use of 2, 4-decadienal for controlling the growth of thistle.
- 2. Use according to claim 1, characterized in that 2, 4-decadienal is used as an attracting active ingredient for attracting soybean thrips.
- 3. The application of a plant source attractant in preventing and controlling the soybean-thistle horse is characterized in that the attractant contains 2, 4-decadienal as an attracting active ingredient, and the concentration of the 2, 4-decadienal in the attractant is 0.1 mu g/mu L-100 mu g/mu L.
- 4. The use according to claim 3, wherein the concentration of 2, 4-decadienal in the attractant is between 0.1 μg/μl and 1 μg/μl.
- 5. The use according to claim 4, wherein the concentration of 2, 4-decadienal in the attractant is 0.1 μg/μl.
- 6. The use according to claim 3, wherein the solvent of the attractant is n-hexane or paraffin oil.
- 7. The use according to any one of claims 1 to 6, wherein the soybean thrips are soybean thrips female and male.
- 8. A method for controlling soybean thrips, which is characterized by comprising the following steps: placing 2, 4-decadienal, the attractant of any one of claims 3-6 or a trap with the attractant of any one of claims 3-6 in a target area to be trapped on soybean thrips.
- 9. The method of claim 8, wherein the trap is a viscous trap.
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| US20090202468A1 (en) * | 2007-12-27 | 2009-08-13 | Renato Ripa Schaul | Attractant compounds for yellow jacket wasps |
| CN109892328A (en) * | 2019-04-25 | 2019-06-18 | 福建省农业科学院植物保护研究所 | A kind of compound for improving thrips female adult and luring rate and indoor egg laying amount |
| CN110604133A (en) * | 2019-08-22 | 2019-12-24 | 浙江省农业科学院 | Gathering pheromone and lure of common thrips and application thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090202468A1 (en) * | 2007-12-27 | 2009-08-13 | Renato Ripa Schaul | Attractant compounds for yellow jacket wasps |
| CN109892328A (en) * | 2019-04-25 | 2019-06-18 | 福建省农业科学院植物保护研究所 | A kind of compound for improving thrips female adult and luring rate and indoor egg laying amount |
| CN110604133A (en) * | 2019-08-22 | 2019-12-24 | 浙江省农业科学院 | Gathering pheromone and lure of common thrips and application thereof |
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| 唐良德;韩云;吴建辉;付步礼;张瑞敏;邱海燕;刘奎;: "豆大蓟马对寄主植物及挥发性化合物的趋性", 环境昆虫学报, no. 05, 25 September 2015 (2015-09-25), pages 1024 - 1029 * |
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