CN118177195A - Composite disinfectant for silkworm rearing room and silkworm rearing tools, and preparation method and application thereof - Google Patents
Composite disinfectant for silkworm rearing room and silkworm rearing tools, and preparation method and application thereof Download PDFInfo
- Publication number
- CN118177195A CN118177195A CN202410298593.8A CN202410298593A CN118177195A CN 118177195 A CN118177195 A CN 118177195A CN 202410298593 A CN202410298593 A CN 202410298593A CN 118177195 A CN118177195 A CN 118177195A
- Authority
- CN
- China
- Prior art keywords
- silkworm
- silkworm rearing
- sodium carbonate
- hydrogen phosphate
- composite disinfectant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000255789 Bombyx mori Species 0.000 title claims abstract description 130
- 239000000645 desinfectant Substances 0.000 title claims abstract description 53
- 230000000384 rearing effect Effects 0.000 title claims abstract description 47
- 239000002131 composite material Substances 0.000 title claims abstract description 34
- 238000002360 preparation method Methods 0.000 title abstract description 11
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims abstract description 130
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims abstract description 66
- 229910000029 sodium carbonate Inorganic materials 0.000 claims abstract description 65
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 29
- 229960000686 benzalkonium chloride Drugs 0.000 claims abstract description 23
- CADWTSSKOVRVJC-UHFFFAOYSA-N benzyl(dimethyl)azanium;chloride Chemical compound [Cl-].C[NH+](C)CC1=CC=CC=C1 CADWTSSKOVRVJC-UHFFFAOYSA-N 0.000 claims abstract description 23
- 239000008213 purified water Substances 0.000 claims abstract description 10
- IEDVJHCEMCRBQM-UHFFFAOYSA-N trimethoprim Chemical compound COC1=C(OC)C(OC)=CC(CC=2C(=NC(N)=NC=2)N)=C1 IEDVJHCEMCRBQM-UHFFFAOYSA-N 0.000 claims description 47
- 229960001082 trimethoprim Drugs 0.000 claims description 47
- 239000000843 powder Substances 0.000 claims description 10
- 239000002994 raw material Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 4
- 239000000084 colloidal system Substances 0.000 claims description 3
- 238000000034 method Methods 0.000 claims description 3
- 238000009472 formulation Methods 0.000 claims 1
- 239000007921 spray Substances 0.000 claims 1
- 230000002147 killing effect Effects 0.000 abstract description 40
- 230000000694 effects Effects 0.000 abstract description 32
- 241000751139 Beauveria bassiana Species 0.000 abstract description 23
- 239000007864 aqueous solution Substances 0.000 abstract description 22
- 230000007797 corrosion Effects 0.000 abstract description 14
- 238000005260 corrosion Methods 0.000 abstract description 14
- 244000052769 pathogen Species 0.000 abstract description 13
- 235000001637 Ganoderma lucidum Nutrition 0.000 abstract description 9
- 240000008397 Ganoderma lucidum Species 0.000 abstract description 9
- 241000701366 unidentified nuclear polyhedrosis viruses Species 0.000 abstract description 9
- 238000004519 manufacturing process Methods 0.000 abstract description 8
- 230000007794 irritation Effects 0.000 abstract description 5
- 239000003814 drug Substances 0.000 description 97
- 229940079593 drug Drugs 0.000 description 52
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 25
- 238000012360 testing method Methods 0.000 description 20
- 241000700605 Viruses Species 0.000 description 16
- 239000008223 sterile water Substances 0.000 description 13
- 238000004659 sterilization and disinfection Methods 0.000 description 13
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 12
- 239000007844 bleaching agent Substances 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 10
- 201000010099 disease Diseases 0.000 description 10
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 10
- 230000009471 action Effects 0.000 description 9
- 239000011859 microparticle Substances 0.000 description 8
- 239000013641 positive control Substances 0.000 description 8
- 235000008708 Morus alba Nutrition 0.000 description 7
- 240000000249 Morus alba Species 0.000 description 7
- 239000000460 chlorine Substances 0.000 description 7
- 229910052801 chlorine Inorganic materials 0.000 description 7
- 150000001875 compounds Chemical class 0.000 description 7
- ZKQDCIXGCQPQNV-UHFFFAOYSA-N Calcium hypochlorite Chemical compound [Ca+2].Cl[O-].Cl[O-] ZKQDCIXGCQPQNV-UHFFFAOYSA-N 0.000 description 6
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 6
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 6
- 229910052802 copper Inorganic materials 0.000 description 6
- 239000010949 copper Substances 0.000 description 6
- 229910052742 iron Inorganic materials 0.000 description 6
- 229910052751 metal Inorganic materials 0.000 description 6
- 239000002184 metal Substances 0.000 description 6
- 229910001220 stainless steel Inorganic materials 0.000 description 6
- 239000010935 stainless steel Substances 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 5
- 235000011941 Tilia x europaea Nutrition 0.000 description 5
- 229910052782 aluminium Inorganic materials 0.000 description 5
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 5
- 239000004571 lime Substances 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 230000001954 sterilising effect Effects 0.000 description 5
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 4
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 4
- 230000000249 desinfective effect Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 241000223679 Beauveria Species 0.000 description 3
- 230000007547 defect Effects 0.000 description 3
- 239000010419 fine particle Substances 0.000 description 3
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 229930040373 Paraformaldehyde Natural products 0.000 description 2
- ZDVDCDLBOLSVGM-UHFFFAOYSA-N [chloro(phenyl)methyl]benzene Chemical compound C=1C=CC=CC=1C(Cl)C1=CC=CC=C1 ZDVDCDLBOLSVGM-UHFFFAOYSA-N 0.000 description 2
- 235000019270 ammonium chloride Nutrition 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- OSVXSBDYLRYLIG-UHFFFAOYSA-N dioxidochlorine(.) Chemical compound O=Cl=O OSVXSBDYLRYLIG-UHFFFAOYSA-N 0.000 description 2
- 230000006806 disease prevention Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229920002866 paraformaldehyde Polymers 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- YRIZYWQGELRKNT-UHFFFAOYSA-N 1,3,5-trichloro-1,3,5-triazinane-2,4,6-trione Chemical compound ClN1C(=O)N(Cl)C(=O)N(Cl)C1=O YRIZYWQGELRKNT-UHFFFAOYSA-N 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- 239000004155 Chlorine dioxide Substances 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 244000137852 Petrea volubilis Species 0.000 description 1
- 241000588769 Proteus <enterobacteria> Species 0.000 description 1
- 206010040047 Sepsis Diseases 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 239000000292 calcium oxide Substances 0.000 description 1
- 235000012255 calcium oxide Nutrition 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 235000019398 chlorine dioxide Nutrition 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- CEJLBZWIKQJOAT-UHFFFAOYSA-N dichloroisocyanuric acid Chemical compound ClN1C(=O)NC(=O)N(Cl)C1=O CEJLBZWIKQJOAT-UHFFFAOYSA-N 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 230000009972 noncorrosive effect Effects 0.000 description 1
- 239000006916 nutrient agar Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 238000005498 polishing Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 229950009390 symclosene Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/12—Quaternary ammonium compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Agronomy & Crop Science (AREA)
- Health & Medical Sciences (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a composite disinfectant for silkworm rearing room and a preparation method and application thereof, wherein the composite disinfectant comprises, by weight, 0.01-0.50% of benzalkonium chloride, 0.05-1.00% of sodium carbonate, 0.05-0.25% of disodium hydrogen phosphate and the balance of purified water, and the aqueous solution is obtained by mixing and dissolving. The composite disinfectant has good killing effect on common pathogens of silkworms such as silkworm nuclear polyhedrosis virus, corpuscle spore, beauveria bassiana, ganoderma lucidum and the like of silkworm tools in a silkworm room, has no corrosion effect on the silkworm tools in the silkworm room and has good stability and no irritation to human bodies, belongs to an environment-friendly disinfectant, and is suitable for wide application in silkworm production.
Description
Technical Field
The invention relates to the technical field of silkworm disease prevention and control. More particularly, the invention relates to a composite disinfectant for silkworm rearing equipment in a silkworm room, a preparation method and application thereof.
Background
The trade amount of cocoons and silks in China is about more than 70% of the world total, serious infectious diseases such as silkworm hematopyosis, beauveria, bacterial diseases, corpuscle diseases and the like frequently occur, the average incidence rate is 10-15%, and the occurrence of silkworm diseases brings great loss to the production of cocoons and seriously damages the healthy development of silkworm industry. The basic guidelines for preventing and treating silkworm diseases are mainly pre-prevention, and mainly comprise the steps of effectively sterilizing the silkworm raising environment, silkworm tools and mulberry leaves by using various disinfectants to prevent the occurrence of silkworm diseases.
The existing disinfectant for silkworm breeding mainly comprises bleaching powder, bleaching powder extract, trichloroisocyanuric acid, sodium dichloroisocyanurate, chlorine dioxide and other high-chlorine preparations, formaldehyde, paraformaldehyde and other formaldehyde, lime. The bleaching powder and the bleaching powder extract are the most widely used medicines for preventing and sterilizing diseases of silkworms, and have strong killing effect on various pathogens, but have the greatest defects of poor stability, easy dissipation of available chlorine, strong irritation and strong corrosiveness, and cause serious corrosion on the silkworms and furniture because of the strong corrosiveness of the disinfectants, namely, the disinfectants with low corrosion but poor disinfection effect are selected by broad silkworm farmers, so that the large-area outbreak of the silkworm diseases is caused by incomplete disinfection. Formaldehyde is a common disinfection drug, and formaldehyde preparation used for disinfection mainly comprises saturated aqueous solution of formaldehyde (formalin) and preparation taking solid paraformaldehyde as main component (such as poison dissipation and disease prevention No. 1), formaldehyde disinfection is comprehensive and thorough, and has low cost, but formaldehyde has the defects of carcinogenesis and cancer promotion, and formalin can only be used in a closed silkworm room capable of heating and has protective equipment, so the use of formaldehyde is limited to a certain extent. Lime is used as a broad disinfectant for silkworm farmers in silkworm production, and has the characteristics of convenient use and low corrosiveness, but quick lime is easy to be naturally oxidized into calcium carbonate in the air, so that the disinfection effect is drastically reduced, the disinfection effect of lime on beauveria bassiana and micro-particle spores is poor, and the pathogen cannot be thoroughly disinfected in production.
Improvements continue to be made based on the above-described deficiencies of existing disinfectants.
Disclosure of Invention
It is an object of the present invention to solve at least the above problems and to provide at least the advantages to be described later.
Aiming at various problems of using disinfection drugs in the current silkworm industry production, such as strong irritation and strong corrosiveness of chlorine-containing preparations, inconvenient use of formaldehyde preparations, cancerogenic and cancer-promoting effects, poor effect of lime on killing beauveria bassiana and corpuscle spores and the like. As a result of various extensive and detailed researches and experiments, the mixed aqueous solution of the trimethoprim ammonium chloride, the sodium bicarbonate and the disodium hydrogen phosphate has strong killing effect on common silkworm pathogens such as silkworm nuclear polyhedrosis virus, microspore, beauveria bassiana, ganoderma lucidum and the like, and the mixed solution has no corrosion effect on iron, stainless steel and copper basically and no pungent smell, belongs to an environment-friendly efficient disinfectant, can perform efficient disinfection on silkworm raising environments and silkworm tools, and can effectively prevent silkworm diseases.
The invention provides a compound disinfectant for silkworm rearing room and silkworm tools, which can effectively kill common pathogens in silkworm industry production such as viruses, bacteria, fungi, microspores and the like, has no corrosion effect on silkworm rearing environment and silkworm rearing tools, can effectively disinfect, and prevents silkworm diseases.
To achieve these objects and other advantages and in accordance with the purpose of the invention, there is provided a composite disinfectant for silkworm rearing room, comprising, by weight, 0.01 to 0.50% of trimethoprim, 0.05 to 1.00% of sodium carbonate, 0.05 to 0.25% of disodium hydrogen phosphate, and the balance of purified water.
Preferably, the composition comprises, by weight, 0.01 to 0.04% of trimethoprim, 0.05 to 0.10% of sodium carbonate, 0.05 to 0.20% of disodium hydrogen phosphate and the balance of purified water.
Preferably, the sodium bicarbonate comprises, by weight, 0.04% of benzalkonium chloride, 0.20% of sodium carbonate, 0.10% of disodium hydrogen phosphate and the balance of purified water.
Preferably, the benzalkonium chloride is a yellow colloid, the sodium carbonate is white powder, and the disodium hydrogen phosphate is white powder.
Preferably, the compound disinfectant is in the form of solution with pH value of 9-12.
The invention also provides a preparation method of the compound disinfectant for the silkworm rearing room, which comprises the steps of adding the raw materials of the trimethoprim, the sodium carbonate and the disodium hydrogen phosphate into purified water in parts by weight, fully dissolving, and filtering to obtain the compound disinfectant.
The invention also provides an application of the composite disinfectant for the silkworm rearing tools, which is characterized in that the silkworm rearing tools are soaked in the composite disinfectant for 10-30min and then taken out and washed clean by clean water, or the composite disinfectant is used for spraying the silkworm rearing tools and closing doors and windows for 10-30min.
Preferably, the compound disinfectant is used for disinfecting the silkworm tools and the silkworm rearing room before and after silkworm rearing, and the compound disinfectant is used for disinfecting the floor of the silkworm rearing room in the process of silkworm rearing.
The invention at least comprises the following beneficial effects:
1. The composite disinfectant for silkworm room and silkworm tools has good killing effect on common silkworm pathogens such as silkworm nuclear polyhedrosis virus, corpuscle spore, beauveria bassiana, ganoderma lucidum and the like, has no corrosion effect on silkworm room and silkworm tools, can achieve thorough disinfection effect, kills the pathogens from the source and prevents silkworm diseases.
2. The composite disinfectant for silkworm rearing room has stable efficacy, has good killing effect on common silkworm pathogens such as silkworm nuclear polyhedrosis virus, microspore, beauveria bassiana, ganoderma lucidum and the like after long-term storage, has the characteristics of convenient long-term storage, convenient transportation and the like, and overcomes the defect that the disinfectant such as bleaching powder, lime and the like is inconvenient to store for a long time and is easy to lose efficacy.
3. The composite disinfectant for the silkworm rearing room and the silkworm rearing tool has low corrosiveness to metal tools, no irritation to human, environmental protection and wide application in silkworm rearing industry production.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Detailed Description
The present invention is described in further detail below with reference to examples to enable those skilled in the art to practice the same by referring to the description.
It will be understood that terms, such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
The experimental methods described in the following embodiments are conventional methods unless otherwise indicated, and the reagents and materials are commercially available.
Comparative example 1
An aqueous solution containing benzalkonium chloride, wherein the content of benzalkonium chloride is 0.04%.
Comparative example 2
An aqueous solution containing sodium carbonate, the content of sodium carbonate being 0.2%.
Comparative example 3
An aqueous solution containing disodium hydrogen phosphate, the content of disodium hydrogen phosphate being 0.10%.
Comparative example 4
An aqueous solution containing benzalkonium chloride and sodium carbonate, wherein the content of benzalkonium chloride is 0.04%, and the content of sodium carbonate is 0.2%.
Comparative example 5
An aqueous solution containing benzalkonium chloride and disodium hydrogen phosphate, wherein the content of benzalkonium chloride is 0.04%, and the content of disodium hydrogen phosphate is 0.10%.
Example 1
A composite disinfectant for silkworm rearing room is prepared from aqueous solution containing 0.01% of benzalkonium chloride, 0.05% of sodium carbonate and 0.10% of disodium hydrogen phosphate.
Example 2
A composite disinfectant for silkworm rearing room is prepared from aqueous solution containing 0.01% of benzalkonium chloride, 0.2% of sodium carbonate and 0.10% of disodium hydrogen phosphate.
Example 3
A composite disinfectant for silkworm rearing room is prepared from aqueous solution containing 0.04% of benzalkonium chloride, 0.05% of sodium carbonate and 0.10% of disodium hydrogen phosphate.
Example 4
A composite disinfectant for silkworm rearing room is prepared from aqueous solution containing 0.04% of benzalkonium chloride, 0.2% of sodium carbonate and 0.10% of disodium hydrogen phosphate.
Example 5
A composite disinfectant for silkworm rearing room is prepared from aqueous solution containing 0.03% of benzalkonium chloride, 0.1% of sodium carbonate and 0.08% of disodium hydrogen phosphate.
Example 6
A composite disinfectant for silkworm rearing room is prepared from aqueous solution containing 0.01% of benzalkonium chloride, 0.05% of sodium carbonate and 0.05% of disodium hydrogen phosphate.
Example 7
A composite disinfectant for silkworm rearing room is prepared from aqueous solution containing 0.50% of benzalkonium chloride, 1.00% of sodium carbonate and 0.25% of disodium hydrogen phosphate.
The preparation method of the medicine comprises the steps of adding raw materials into purified water, and fully dissolving the raw materials according to the weight content. The raw materials are purchased in the same batch so as to facilitate the comparison of test effects, the trimethoprim ammonium chloride is yellow colloid, the sodium carbonate is white powder, and the disodium hydrogen phosphate is white powder.
Test of efficacy
Nuclear polyhedrosis virus killing test
The medicines of comparative examples 1 to 5 and examples 1 to 7 and a control medicine (containing 1% of an aqueous solution of available chlorine bleach powder) were subjected to a silkworm nuclear polyhedron virus killing test, and the effect of the medicines on virus killing was investigated, specifically by the following steps:
the method for investigating the killing effect of the medicine on viruses by the combined action of the medicine on viruses comprises the following specific steps:
And (3) drug treatment: each treatment group was set with 3 replicates, 50 silkworms each. Preparing 1X 10 8 pieces/mL of silkworm core polyhedrosis virus (NPV), taking 1mL of virus liquid, respectively adding into test medicines, immediately centrifuging for 10min, centrifuging for 2000 revolutions/min, centrifuging for 10min, removing supernatant, adding 5mL of sterile water, shaking uniformly, smearing on fresh mulberry leaves, feeding on 2-year old silkworms, feeding on normal mulberry leaves after 5h, continuously investigating for 4d after the disease of a medicine group (3-4 d after infection), counting the number of the silkworms with blood type sepsis, and calculating the medicine protection rate.
Control treatment: 2 control groups are respectively arranged, namely a positive control group and a blank control group, wherein the positive control group is directly fed with silkworms after virus treatment by using sterile water instead of medicines, and the blank control group is directly fed with silkworms by smearing mulberry leaves with the sterile water.
The disinfection effect of the medicine is expressed by the protection rate, and the specific calculation formula is as follows:
TABLE 1 disinfecting effect of drugs on Nuclear polyhedrosis Virus
Table 1 shows that medicine 2 (0.20% sodium carbonate solution), medicine 4 (0.04% benzalkonium chloride, 0.20% sodium carbonate), medicine 7 (0.01% benzalkonium chloride, 0.20% sodium carbonate, 0.10% disodium hydrogen phosphate solution), medicine 9 (0.04% benzalkonium chloride, 0.2% sodium carbonate, 0.10% disodium hydrogen phosphate) and medicine 12 (0.50% benzalkonium chloride, 1.00% sodium carbonate, 0.25% disodium hydrogen phosphate) have good killing effect on silkworm nuclear polyhedrosis virus, the effect difference between the 4 medicines is not large, after 5min of effect, the protection rate on silkworms is about 80%, after 10min of effect, the viruses can be completely killed, and the protection rate on silkworms reaches 100%.
The medicine 10 (0.03% of trimethoprim, 0.1% of sodium carbonate and 0.08% of disodium hydrogen phosphate) has better killing effect on the silkworm nuclear polyhedrosis virus, and the protection rate of the silkworm after 5min, 10min and 20min is 60%, 90% and 100% respectively.
Drug 6 (0.01% of trimethoprim, 0.05% of sodium carbonate and 0.10% of disodium hydrogen phosphate), drug 8 (0.04% of trimethoprim, 0.05% of sodium carbonate and 0.10% of disodium hydrogen phosphate) and drug 11 (0.01% of trimethoprim, 0.05% of sodium carbonate and 0.05% of disodium hydrogen phosphate) have poor killing effect on nuclear polyhedron, and have 20min of action, and the protection rate on silkworms is 10%, 18% and 18% respectively; drug 1 (0.04% benzhydryl chloride), drug 3 (0.1% disodium hydrogen phosphate), drug 5 (0.04% benzhydryl chloride, 0.10% disodium hydrogen phosphate) had no killing effect on nuclear polyhedrosis.
Fine particle spore killing test
The medicines of comparative examples 1 to 5 and examples 1 to 7 and a control medicine (containing 1% of an aqueous solution of available chlorine bleach powder) were subjected to a fine particle spore killing test, and the effect of the medicines on fine particle spores was investigated, specifically by the following steps:
and (3) drug treatment: each treatment group was set with 3 replicates, 50 silkworms each. Preparing 1X 10 8/mL of microspores (Nb), taking 1mL of Nb solution, respectively adding into test medicines, immediately centrifuging for 10min, centrifuging for 2000 rpm for 10min, removing supernatant, adding 5mL of sterile water, shaking uniformly, smearing on fresh mulberry leaves, feeding on 2-year old silkworm, feeding with normal mulberry leaves after 5h, feeding to 5-year old silkworm, checking silkworm microparticle infection condition with a microscope, and calculating the medicine protection rate.
Control treatment: 2 control groups are respectively arranged, namely a positive control group and a blank control group, wherein the positive control group is directly fed with silkworms after virus treatment by using sterile water instead of medicines, and the blank control group is directly fed with silkworms by smearing mulberry leaves with the sterile water.
The disinfection effect of the medicine is expressed by the protection rate, and the specific calculation formula is as follows:
Table 2 disinfecting effect of the drug on silkworm microspores
Table 2 shows that medicine 4 (0.04% of trimethoprim, 0.20% of sodium carbonate), medicine 9 (0.04% of trimethoprim, 0.2% of sodium carbonate, 0.10% of disodium hydrogen phosphate) and medicine 12 (0.50% of trimethoprim, 1.00% of sodium carbonate, 0.25% of disodium hydrogen phosphate) have good killing effect on silkworm microparticle spores, the effect is 10min, the microparticle spores are completely killed, and the medicine protection rate reaches 100%. Wherein, the effect of the medicine 9 and the medicine 12 is optimal, and the micro-particle spores can be completely killed after 5min of the effect, and the protection rate reaches 100 percent.
The medicine 10 (0.03% of trimethoprim, 0.1% of sodium carbonate and 0.08% of disodium hydrogen phosphate) has good killing effect on silkworm microparticle spores, and the protection rate of the silkworm after 5min, 10min and 20min is 60%, 85% and 100% respectively.
Drug 1 (0.04% of trimethoprim), drug 5 (0.04% of trimethoprim, 0.10% of disodium hydrogen phosphate), drug 6 (0.01% of trimethoprim, 0.05% of sodium carbonate, 0.10% of disodium hydrogen phosphate), drug 7 (0.01% of trimethoprim, 0.2% of sodium carbonate, 0.10% of disodium hydrogen phosphate) and drug 8 (0.04% of trimethoprim, 0.05% of sodium carbonate, 0.10% of disodium hydrogen phosphate) have poor effect of killing silkworm micro-particle spores, and after 20min of action, the protection rates of the silkworm are 20%, 25%, 23%, 25% and 40%, respectively; drug 2 (0.20% sodium carbonate) and drug 3 (0.10% disodium hydrogen phosphate) had no killing effect on silkworm microspores.
Beauveria bassiana killing test
The medicines of comparative examples 1 to 5 and examples 1 to 7 and the control medicine (containing 1% of the effective chlorine bleach powder aqueous solution) were subjected to a beauveria killing test, and the effect of the medicines on killing beauveria was investigated, specifically as follows:
And (3) drug treatment: preparing 1X 10 8/mL beauveria bassiana, taking 1mL beauveria bassiana liquid, respectively adding into test medicines, immediately centrifuging for 10min, centrifuging for 2000 r/min, centrifuging for 10min, removing supernatant, adding 5mL of sterile water, shaking uniformly, smearing a potato culture medium plate, placing into a constant temperature incubator, culturing at 28 ℃ for 72h, and observing the growth condition of beauveria bassiana. Each treatment group was set to 3 replicates.
Control treatment: 2 control groups, positive control group and blank control group, respectively, are set, the positive control group uses sterile water to replace drug to treat virus, and the blank control group uses sterile water to replace virus.
TABLE 3 sterilizing effect of drug on Beauveria bassiana
Note that: "-" means sterile growth; "+" indicates l-5 colonies; "++" indicates 6 to 30 colonies; "+". ++'s representation of more than 30 colonies; "+". ++ + "means unable to count
Table 3 shows that medicine 1 (0.04% of trimethoprim), medicine 4 (0.04% of trimethoprim, 0.20% of sodium carbonate), medicine 5 (0.04% of trimethoprim, 0.10% of disodium hydrogen phosphate), medicine 8 (0.04% of trimethoprim, 0.05% of sodium carbonate, 0.10% of disodium hydrogen phosphate), medicine 9 (0.04% of trimethoprim, 0.2% of sodium carbonate, 0.10% of disodium hydrogen phosphate) and medicine 12 have good killing effect on beauveria bassiana, and beauveria bassiana can be completely killed after 10 minutes of action. Wherein, the medicine 9 and the medicine 12 (0.50% of trimethoprim, 1.00% of sodium carbonate and 0.25% of disodium hydrogen phosphate) have the best killing effect on the beauveria bassiana, and can completely kill the beauveria bassiana after 5min of action.
The medicine 10 (0.03% of trimethoprim, 0.1% of sodium carbonate and 0.08% of disodium hydrogen phosphate) has good effect of killing the beauveria bassiana, and can completely kill the beauveria bassiana after 20 minutes of action.
Drug 6 (0.01% of trimethoprim, 0.05% of sodium carbonate and 0.10% of disodium hydrogen phosphate), drug 7 (0.01% of trimethoprim, 0.2% of sodium carbonate and 0.10% of disodium hydrogen phosphate) and drug 11 (0.01% of trimethoprim, 0.05% of sodium carbonate and 0.05% of disodium hydrogen phosphate) have certain inhibition effects on beauveria bassiana, but the effects are poor, and the beauveria bassiana is not completely killed after 20 minutes of action; drug 2 (0.20% sodium carbonate) and drug 3 (0.10% disodium hydrogen phosphate) had no killing effect on beauveria bassiana.
Test for killing Proteus
The medicines of comparative examples 1 to 5 and examples 1 to 7 and the control medicine (containing 1% of available chlorine bleach powder aqueous solution) were subjected to a ganoderma lucidum killing test, and the effect of the medicines on ganoderma lucidum killing was investigated, specifically by the following steps:
And (3) drug treatment: preparing 1X 10 8 bacteria liquid per mL, taking 1mL bacteria liquid, respectively adding into test medicines, immediately centrifuging for 10min, centrifuging for 2000r/min, centrifuging for 10min, removing supernatant, adding sterile water 5mL, shaking uniformly, smearing nutrient agar culture medium plate, placing into a constant temperature incubator, culturing at 37deg.C for 24h, and observing growth condition of bacteria.
Each treatment group was set with 3 replicates
Control treatment: 2 control groups, positive control group and blank control group, respectively, are set, the positive control group uses sterile water to replace drug to treat virus, and the blank control group uses sterile water to replace virus.
TABLE 4 sterilizing effect of the drug on Ganoderma lucidum
Note that: "-" means sterile growth; "+" indicates l-5 colonies; "++" indicates 6 to 30 colonies; "+". ++'s representation of more than 30 colonies; "+". ++ + "means unable to count
Table 4 shows that medicine 1 (0.04% of trimethoprim), medicine 4 (0.04% of trimethoprim, 0.20% of sodium carbonate), medicine 5 (0.04% of trimethoprim, 0.10% of disodium hydrogen phosphate), medicine 8 (0.04% of trimethoprim, 0.05% of sodium carbonate, 0.10% of disodium hydrogen phosphate), medicine 9 (0.04% of trimethoprim, 0.2% of sodium carbonate, 0.10% of disodium hydrogen phosphate) and medicine 12 have good killing effect on silkworm, and the silkworm can be completely killed after 10 minutes of action; wherein, the medicine 9 (0.04% of trimethoprim, 0.2% of sodium carbonate and 0.10% of disodium hydrogen phosphate) and the medicine 12 (0.50% of trimethoprim, 1.00% of sodium carbonate and 0.25% of disodium hydrogen phosphate) have the best killing effect on the silkworm bacteria, and can be completely killed after 5min of action.
The medicine 10 (0.03% of trimethoprim, 0.1% of sodium carbonate and 0.08% of disodium hydrogen phosphate) has good effect of killing silkworm and can completely kill the silkworm after 20 minutes.
Drug 6 (0.01% of trimethoprim, 0.05% of sodium carbonate and 0.10% of disodium hydrogen phosphate), drug 7 (0.01% of trimethoprim, 0.2% of sodium carbonate and 0.10% of disodium hydrogen phosphate) and drug 11 (0.01% of trimethoprim, 0.05% of sodium carbonate and 0.05% of disodium hydrogen phosphate) have a certain inhibition effect on bombyx mori, but the effect is poor, and the bombyx mori is not completely killed after 20 minutes of effect; drug 2 (0.20% sodium carbonate) and drug 3 (0.10% disodium hydrogen phosphate) had no killing effect on bombyx mori.
The comprehensive nuclear polyhedrosis virus killing test, the microparticle spore killing test, the beauveria bassiana killing test and the lingjun killing test result show that the medicine 9 (0.04% of trimethoprim, 0.2% of sodium carbonate and 0.10% of disodium hydrogen phosphate) and the medicine 12 (0.50% of trimethoprim, 1.00% of sodium carbonate and 0.25% of disodium hydrogen phosphate) have good killing effects on 4 common silkworm pathogens, and can completely kill the pathogens after 10 minutes of the pathogen.
Corrosion test
Polishing impurities on the surfaces of iron sheets, stainless steel sheets, aluminum sheets and copper sheet samples by sand paper, washing the samples by clean water, and then placing the samples in a 50 ℃ oven for drying for 1 hour, and accurately weighing (M); then, respectively placing the metal samples into comparative examples 1-3, example 4, example 5, example 6, example 7, 1% bleaching powder solution and sterile water for 3d, and replacing the new disinfectant for 1 time a day; and taking out stainless steel sheet, iron sheet, aluminum sheet and copper sheet samples after soaking for 3d, washing the samples with clear water, putting the samples into a 50 ℃ oven again, drying the samples for 1h, accurately weighing (Mt), and calculating the corrosion rate (R).
The corrosion rate calculation formula: r=8.76×10 4 × (M-Mt)/(s×t×d)
Remarks: r is the corrosion rate of the metal, M and Mt are the weight (g) of the sample before and after the test, respectively, and S is the sheet surface area (cm 2) of the metal sample; t is soaking time (h); d is the density of the metal (g/cm 3).
TABLE 5 corrosiveness of drugs to different metals
Remarks: the corrosion grading standard R is less than 0.005 and is corrosion-free; r is more than or equal to 0.005 and less than 0.010, and is basically free of corrosion, R is more than or equal to 0.010 and less than or equal to 0.100, and is slightly corroded, R is more than or equal to 0.100 and less than or equal to 1.00, and R is more than or equal to 1.00 and is slightly corroded.
From Table 5, it is shown that 0.04% benzalkonium chloride and 0.10% disodium hydrogen phosphate alone in water are non-corrosive to iron, stainless steel, aluminum, copper; however, 0.2% sodium carbonate in water is moderately corrosive to aluminum; the aqueous solution of the trimethoprim, the sodium carbonate and the disodium hydrogen phosphate prepared by the invention has no corrosiveness to iron, stainless steel and copper, can reduce corrosion grade to aluminum, and improves the safety of sterilizing silkworms in a silkworm room.
The compound disinfectant has good killing effect on common silkworm pathogens such as viruses, microspores, beauveria bassiana, ganoderma lucidum and the like in silkworm rearing room, particularly an aqueous solution containing 0.04% of trimethoprim, 0.2% of sodium carbonate and 0.10% of disodium hydrogen phosphate and an aqueous solution containing 0.50% of trimethoprim, 1.00% of sodium carbonate and 0.25% of disodium hydrogen phosphate can completely kill the nuclear polyhedron viruses, the microspores, the beauveria bassiana and the ganoderma lucidum of the silkworm after being acted for 10min, and the disinfectant composition has good disinfection effect on main silkworm pathogens, has no corrosion on iron, stainless steel and copper, has good stability and no irritation on human bodies, and belongs to an environment-friendly disinfectant, and is suitable for wide application in silkworm industry production.
The number of equipment and the scale of processing described herein are intended to simplify the description of the present invention. Applications, modifications and variations of the present invention will be readily apparent to those skilled in the art.
Although embodiments of the present invention have been disclosed above, it is not limited to the details and embodiments shown, it is well suited to various fields of use for which the invention is suited, and further modifications may be readily made by one skilled in the art, and the invention is therefore not to be limited to the particular details and examples shown and described herein, without departing from the general concepts defined by the claims and the equivalents thereof.
Claims (8)
1. The composite disinfectant for silkworm rearing room and silkworm rearing tool is characterized by comprising, by weight, 0.01-0.50% of trimethoprim, 0.05-1.00% of sodium carbonate, 0.05-0.25% of disodium hydrogen phosphate and the balance of purified water.
2. The composite disinfectant for silkworm rearing room according to claim 1, which comprises, by weight, 0.01 to 0.04% of trimethoprim, 0.05 to 0.20% of sodium carbonate, 0.05 to 0.10% of disodium hydrogen phosphate, and the balance of purified water.
3. The composite disinfectant for silkworm rearing room according to claim 2, which comprises, in weight percent, 0.04% of trimethoprim, 0.20% of sodium carbonate, 0.10% of disodium hydrogen phosphate and the balance of purified water.
4. The composite disinfectant for silkworm rearing room according to claim 3, wherein the benzalkonium chloride is a yellow colloid, the sodium carbonate is white powder, and the disodium hydrogen phosphate is white powder.
5. The composite disinfectant for silkworm rearing room according to any one of claims 1 to 4, wherein the formulation of the composite disinfectant is a solution, and the PH of the solution is 9 to 12.
6. A method for preparing a composite disinfectant for silkworm rearing in a silkworm room, characterized in that the raw materials according to any one of claims 1 to 4 are added into purified water in amounts of benzalkonium chloride, sodium carbonate and disodium hydrogen phosphate, and the mixture is filtered after being sufficiently dissolved, thereby obtaining the composite disinfectant.
7. Application of a composite disinfectant for silkworm rearing equipment, characterized in that the silkworm rearing equipment is immersed in the composite disinfectant according to any one of claims 1 to 4 for 10 to 30 minutes and then taken out and washed clean with clean water, or the composite disinfectant is used to spray the silkworm rearing equipment and the door and window are closed for 10 to 30 minutes.
8. The use of the composite disinfectant for silkworm rearing room and silkworm rearing room according to claim 7, wherein the silkworm rearing room and the silkworm rearing room are sterilized with the composite disinfectant before and after rearing, and the floor of the silkworm rearing room is sterilized with the composite disinfectant during rearing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410298593.8A CN118177195A (en) | 2024-03-15 | 2024-03-15 | Composite disinfectant for silkworm rearing room and silkworm rearing tools, and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410298593.8A CN118177195A (en) | 2024-03-15 | 2024-03-15 | Composite disinfectant for silkworm rearing room and silkworm rearing tools, and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN118177195A true CN118177195A (en) | 2024-06-14 |
Family
ID=91414678
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410298593.8A Pending CN118177195A (en) | 2024-03-15 | 2024-03-15 | Composite disinfectant for silkworm rearing room and silkworm rearing tools, and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN118177195A (en) |
-
2024
- 2024-03-15 CN CN202410298593.8A patent/CN118177195A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP3863913B2 (en) | Permanent antibacterial agent | |
| US8226993B2 (en) | Non-toxic anti microbial composition | |
| CN102077828A (en) | Compound disinfectant containing quaternary ammonium salt | |
| CN106234388B (en) | A kind of composition pesticide of alkene containing benzo fluorine bacterium azoles and jamaicin | |
| CN108633888A (en) | A kind of sodium dichloroisocyanuratepowder powder and preparation method thereof | |
| CN118177195A (en) | Composite disinfectant for silkworm rearing room and silkworm rearing tools, and preparation method and application thereof | |
| Sutio et al. | Serratia marcescens strain NPKC3_2_21 as endophytic phosphate solubilizing bacteria and entomopathogen: Promising combination approach as rice biofertilizer and biopesticide. | |
| CN104886110B (en) | A kind of multiduty composite cation surfactant disinfectants | |
| WO2021196111A1 (en) | Broad-spectrum antiviral bactericidal plastic and application thereof | |
| CN103392708B (en) | Special disinfectant for artificial diet rearing of silkworms and application thereof | |
| JP2011074047A (en) | Process of humus extract | |
| CN111328811B (en) | Low-concentration alcohol sterilization disinfectant and application thereof | |
| CN115109665A (en) | Safe and environment-friendly disinfection and sterilization laundry detergent and preparation method thereof | |
| CN113475530A (en) | Stable disinfectant and preparation method thereof | |
| CN1061817C (en) | Disinfectant for silkworm | |
| CN112205401B (en) | Disinfectant for silkworm and use method thereof | |
| CN117814239A (en) | Composite antibacterial disinfectant containing modified chitosan and preparation method thereof | |
| CN113854299B (en) | Application of 2, 5-substituted furan compounds and/or metal complexes thereof in antibacterial and mildew-proof disinfection field | |
| CN101144247A (en) | Special-purpose avian influenza resisting finishing agent for eiderdown and preparation method thereof | |
| CN107079915A (en) | Composite bactericide and its application containing fluoxastrobin and jamaicin | |
| CN113519567A (en) | Preparation method of disinfectant for medical instruments | |
| CN114916560A (en) | Composition for preventing and treating bacterial diseases of edible fungi, preparation and preparation method thereof | |
| CN1631161A (en) | Disinfecting agent used for silkworm containing stable chlorine dioxide | |
| CN105123701A (en) | Mulberry leaf disinfecting medicine composition, preparation method and application thereof | |
| CN105820884A (en) | Cheep, efficient and pollution-free sodium ferrate toilet cleaning antibacterial sustained-release material and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination |