CN118104823A - Preparation method of sea buckthorn oily extract with repairing effect - Google Patents
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Abstract
The invention belongs to the field of deep processing of agricultural products, and discloses a preparation method of a sea buckthorn oily extract with a repairing effect. The preparation method of oleum Hippophae extract comprises homogenizing fresh fructus Hippophae, adding pectase, cellulase and beta-glucanase, and performing enzymolysis; after enzymolysis is completed, carrying out ultrasonic treatment, carrying out solid-liquid separation, removing slag, and further separating liquid to obtain an oil phase and a water phase; filtering the oil phase to obtain refined oil, concentrating the water phase, and drying to obtain water extract; mixing the refined oil and the water extract uniformly to obtain the sea buckthorn oily extract. According to the preparation method of the sea buckthorn oily extract, disclosed by the embodiment of the invention, sea buckthorn seeds and sea buckthorn pulps are extracted simultaneously, so that the pretreatment of sea buckthorn is simplified, meanwhile, the active ingredients of sea buckthorn can be comprehensively extracted, and the prepared sea buckthorn oily extract has good restoration effect, can be used for foods, skin care products, cosmetics and the like, and comprises but is not limited to products such as color cosmetics, skin care products and washing products.
Description
Technical Field
The invention belongs to the field of deep processing of agricultural products, and particularly relates to a preparation method of a sea buckthorn oily extract with a repairing effect.
Background
Sea buckthorn, also known as sea red fruit, hippophae rhamnoides, is a deciduous shrub or small arbor, belonging to the genus Hippophae of the family Elaeagnaceae. The plant is mainly distributed in the middle coast of Mediterranean to the middle part of Asia, and in China, the plant is mainly distributed in North China, northwest China, southwest and other places. The fructus Hippophae has a round ball shape with a diameter of 4-6 mm, and is orange or red. Sea buckthorn is not only a plant with high economic value, but also plays an important role in water and soil conservation, wind prevention, sand fixation and the like.
The sea buckthorn fruits contain rich nutrients and bioactive substances, especially very high vitamin C content, and are known as vitamin C king. The fructus Hippophae can be used as food material, and can be used for preparing health tea, jam, and confection. In addition, the roots, stems, leaves and flowers of the sea buckthorn have high utilization value, and can be widely applied to the fields of food, medicine, light industry, aerospace, agriculture, animal husbandry and the like. The sea buckthorn fruit has the effects of relieving cough and reducing sputum, invigorating stomach and promoting digestion, and promoting blood circulation and removing blood stasis, and modern medical research also shows that the sea buckthorn can reduce cholesterol and relieve angina pectoris, and has the effect of preventing and treating coronary atherosclerotic heart disease.
Among the numerous extracts of seabuckthorn, seabuckthorn seed oil has various effects including eliminating phlegm, benefiting lung, resolving dampness, anti-infection, promoting healing and the like. The total flavonoids of sea buckthorn contained in the composition can capture superoxide radicals and hydroxyl radicals, has the functions of resisting oxidization and eliminating radicals on cell membranes, and can effectively delay human aging. In addition, the seabuckthorn seed oil can supplement nutrition, such as vitamin E, carotene, carotenoid, beta-sitosterol and the like, contains a large amount of amino acids, organic acids and other various nutritional components, can promote gastric acid synthesis, stimulates gastric secretion, and has the effects of promoting digestion, resolving food stagnation, strengthening spleen, nourishing stomach, soothing liver and benefiting qi. The organic acid such as malic acid, oxalic acid and the like contained in the composition has the effect of relieving the toxicity of antibiotics and other medicines, and can effectively protect the liver. The sea buckthorn seed oil can also form an oily protective film on a wound surface, shield the wound surface, has quick local permeation and absorption, resists inflammation and pain, nourishes epithelial mucosa, contains abundant vitamins and amino acids, can promote collagen synthesis, resists injury, prevents infection, and reduces inflammation and pain. In the prior art, the deep processing of sea buckthorn generally mainly comprises the extraction of sea buckthorn seed oil.
CN112159712a discloses a method for extracting sea buckthorn oil, which comprises the following steps: ultrasonic processing is carried out on sea buckthorn fruits, and then drying and crushing are carried out in sequence, so that ultrasonic sea buckthorn fruit powder is obtained; and extracting the ultrasonic seabuckthorn fruit powder to obtain seabuckthorn oil. CN106635378a discloses a method for extracting fruit oil from sea buckthorn fruit puree. A method for extracting oleum Hippophae from fructus Hippophae mud comprises adding 0.05-0.1%of compound pectase into fructus Hippophae mud, performing enzymolysis at 45-50deg.C for 30min, and separating oleum Hippophae by combination of centrifugal separation and supercritical CO 2 extraction; and step two, degumming, deacidifying, decoloring and deodorizing the sea buckthorn fruit oil obtained by the separation in the step one. CN101732371a discloses a method for co-producing and extracting fruit oil and polysaccharide in sea buckthorn fruit puree, which comprises the following steps: (1) thickening; (2) juice separation; (3) fruit puree oil separation; (4) solid-liquid separation; (4) deacidifying; (5) decoloring; (6) Deodorizing to obtain refined sea buckthorn fruit oil with a yield of 50%. CN101705145A discloses a new method for separating and extracting sea buckthorn fruit oil, which mainly adopts a method of combining bioengineering enzyme technology, liquid high-speed gravity separation technology and microporous filtration technology to separate and extract sea buckthorn fruit oil from sea buckthorn fruits, and because a plurality of enzyme preparations such as cell wall breaking enzyme, pectase, protease and the like are adopted to carry out biocatalysis reaction in the processing process, the coupling bond among substances is broken, so that substances such as pectin, protein, cellulose, hemicellulose, fat and the like in sea buckthorn fruits can be fully decomposed, original nutritional ingredients in sea buckthorn fruits are uniformly dispersed in fruit pulp, and the quality of sea buckthorn juice is improved while the sea buckthorn fruit oil is separated to the maximum extent.
In the prior art, the sea buckthorn fruit oil and other components are extracted respectively, the extraction process is complex, and the loss of active ingredients of the components is large.
Disclosure of Invention
The invention aims to overcome at least one defect of the prior art and provides a preparation method of a sea buckthorn oily extract with a repairing effect.
The technical scheme adopted by the invention is as follows:
in a first aspect of the invention, there is provided:
A preparation method of oleum Hippophae extract comprises the following steps:
homogenizing fresh fructus Hippophae, adding pectase, cellulase and beta-glucanase, and performing enzymolysis;
After enzymolysis is completed, carrying out ultrasonic treatment, carrying out solid-liquid separation, removing slag, and further separating liquid to obtain an oil phase and a water phase;
Filtering the oil phase to obtain refined oil, concentrating the water phase, and drying to obtain water extract;
mixing the refined oil and the water extract uniformly to obtain the sea buckthorn oily extract.
In some examples of the preparation method, the mass mixing ratio of the fresh sea buckthorn fruit to the water is 1: (0.5 to 5), preferably 1: (1-3). Experimental data shows 1: the mixing ratio of (1-3) is advantageous in improving the yield.
In some examples of the preparation method, fresh seabuckthorn fruit is homogenized to 30-50 mesh. This is advantageous in that the productivity is improved and the residue can be removed more conveniently.
In some examples of the preparation method, the pectinase: cellulase: beta-glucanase = 100U: (20-40) U: (3-6) U.
In some examples of the preparation method, the mixing ratio of fresh seabuckthorn fruit to pectase is 1 kg fresh seabuckthorn fruit: (10000-20000) U.
In some examples of the preparation method, the time of the enzymolysis treatment is 3 to 5 hours.
In some examples of the preparation method, the temperature of the enzymolysis treatment is 40-60 ℃.
In some examples of the preparation method, the pH of the enzymatic treatment is 3.5 to 6.0.
In some examples of the preparation method, the time of the ultrasonic treatment is 30 to 60 min.
In some examples of the preparation process, the aqueous phase is concentrated to dryness at a temperature of no more than 60 ℃.
In some examples of the preparation process, the oil phase is refined using ceramic membrane filtration and the aqueous phase is concentrated using membrane separation and then dried.
In some examples of the preparation process, the process may be carried out,
In a second aspect of the invention, there is provided: the sea buckthorn oil extract prepared by the preparation method according to the first aspect of the invention.
In a third aspect of the invention, there is provided: a composition comprises an oily extract of Hippophae rhamnoides according to the second aspect of the invention.
In some examples of compositions, they are foods, skin care products, cosmetics.
The beneficial effects of the invention are as follows:
according to the preparation method of the sea buckthorn oily extract, disclosed by the embodiment of the invention, sea buckthorn seeds and sea buckthorn pulps are extracted simultaneously, so that the pretreatment of sea buckthorn is simplified, meanwhile, the active ingredients of sea buckthorn can be comprehensively extracted, and the prepared sea buckthorn oily extract has good restoration effect, can be used for foods, skin care products, cosmetics and the like, and comprises but is not limited to products such as color cosmetics, skin care products and washing products.
According to the preparation method of the sea buckthorn oily extract, disclosed by the invention, the cell wall and pectin can be effectively destroyed by using specific complex enzyme, meanwhile, the viscosity of enzymolysis liquid is effectively reduced, the operation is greatly facilitated, and the higher yield is obtained.
Drawings
FIG. 1 is a photograph of refined sea buckthorn oil prepared in example 2.
Detailed Description
A preparation method of oleum Hippophae extract comprises the following steps:
homogenizing fresh fructus Hippophae, adding pectase, cellulase and beta-glucanase, and performing enzymolysis;
After enzymolysis is completed, carrying out ultrasonic treatment, carrying out solid-liquid separation, removing slag, and further separating liquid to obtain an oil phase and a water phase;
Filtering the oil phase to obtain refined oil, concentrating the water phase, and drying to obtain water extract;
mixing the refined oil and the water extract uniformly to obtain the sea buckthorn oily extract.
In some examples of the preparation method, the mass mixing ratio of the fresh sea buckthorn fruit to the water is 1: (0.5 to 5), preferably 1: (1-3).
In some examples of the preparation method, fresh seabuckthorn fruit is homogenized to 30-50 mesh.
In some examples of the preparation method, the pectinase: cellulase: beta-glucanase = 100U: (20-40) U: (3-6) U. The data show that at this mixing ratio, it is advantageous to increase the yield.
In some examples of the preparation method, the mixing ratio of fresh seabuckthorn fruit to pectase is 1 kg fresh seabuckthorn fruit: (10000-20000) U. At this mixing ratio, not only can the requirements of enzymolysis be satisfied, but also the adverse effect of excessive use on the yield can be avoided, and simultaneously, the method has better economical efficiency.
In some examples of the preparation method, the pectinase: cellulase: beta-glucanase = 100U: (20-40) U: (3-6) U, wherein the mixing ratio of the fresh sea buckthorn fruits and the pectase is 1 kg: (10000-20000) U.
In some examples of the preparation method, the time of the enzymolysis treatment is 3 to 5 hours. The method can be specifically and correspondingly adjusted according to the enzymolysis degree.
In some examples of the preparation method, the temperature of the enzymolysis treatment is 40-60 ℃. The specific temperature may be adjusted according to the optimal enzyme activity temperature of the enzyme. With a more desirable enzymatic hydrolysis rate, lower enzymatic hydrolysis temperatures are beneficial in avoiding loss of active ingredients.
In some examples of the preparation method, the pH of the enzymatic treatment is 3.5 to 6.0. The specific pH may be adjusted according to the pH of the enzyme's optimal enzyme activity.
In some examples of the preparation method, the time of the ultrasonic treatment is 30 to 60 min. This facilitates sufficient release of the extract.
In some examples of the preparation process, the aqueous phase is concentrated to dryness at a temperature of no more than 60 ℃. This is advantageous in avoiding loss of active ingredient.
In some examples of the preparation process, the oil phase is refined using ceramic membrane filtration and the aqueous phase is concentrated using membrane separation and then dried.
The technical scheme of the invention is further described below by combining examples.
The fresh seabuckthorn fruits used in the following examples were all high quality fresh seabuckthorn fruits produced in the same batch from the plateau area on the Qinghai altitude 2300 m unless otherwise specified. The mixing ratios are, for example, mass mixing ratios unless otherwise specified.
Example 1:
A preparation method of oleum Hippophae extract comprises the following steps:
Sea buckthorn fresh fruits and water are mixed according to the proportion of 1:1, homogenizing to about 40 meshes, adding pectase 1000U, cellulase 300U and beta-glucanase 45U into each 100g of fresh sea buckthorn fruit, mixing and carrying out enzymolysis, wherein the enzymolysis temperature is 40-45 ℃, the pH is 3.5-4.0, and the total enzymolysis time is controlled to be 4 hours;
after the enzymolysis is finished, carrying out ultrasonic treatment on the mixture 45 min, carrying out solid-liquid separation, removing residues, and further separating the liquid to obtain an oil phase and a water phase;
filtering and refining the oil phase with ceramic membrane to obtain refined oil, separating and concentrating the water phase with membrane, and drying at no more than 60deg.C to obtain water extract;
mixing the refined oil and the water extract uniformly to obtain the sea buckthorn oily extract.
Example 2:
A preparation method of oleum Hippophae extract comprises the following steps:
sea buckthorn fresh fruits and water are mixed according to the proportion of 1:2, homogenizing to about 30 meshes, adding pectase 1500U, cellulase 600U and beta-glucanase 45U into each 100g of fresh sea buckthorn fruit, mixing and carrying out enzymolysis at 43-48 ℃ and pH 4.0-4.5, and controlling the total enzymolysis time to be 4 hours;
After the enzymolysis is finished, carrying out ultrasonic treatment on the mixture 40 min, carrying out solid-liquid separation, removing residues, and further separating the liquid to obtain an oil phase and a water phase;
filtering and refining the oil phase with ceramic membrane to obtain refined oil, separating and concentrating the water phase with membrane, and drying at no more than 60deg.C to obtain water extract;
mixing the refined oil and the water extract uniformly to obtain the sea buckthorn oily extract.
Example 3:
A preparation method of oleum Hippophae extract comprises the following steps:
Sea buckthorn fresh fruits and water are mixed according to the proportion of 1: mixing in a mass ratio of 0.5, homogenizing to about 50 meshes, adding pectase 2000U, cellulase 400U and beta-glucanase 100U into each 100g of fresh sea buckthorn fruit, mixing and carrying out enzymolysis, wherein the enzymolysis temperature is 50-60 ℃, the pH is 3.5-4.0, and the total enzymolysis time is controlled to be 5 hours;
after the enzymolysis is finished, carrying out ultrasonic treatment on the mixture 60 min, carrying out solid-liquid separation, removing residues, and further separating the liquid to obtain an oil phase and a water phase;
filtering and refining the oil phase with ceramic membrane to obtain refined oil, separating and concentrating the water phase with membrane, and drying at no more than 60deg.C to obtain water extract;
mixing the refined oil and the water extract uniformly to obtain the sea buckthorn oily extract.
Example 4:
A preparation method of oleum Hippophae extract comprises the following steps:
sea buckthorn fresh fruits and water are mixed according to the proportion of 1:5, homogenizing to about 50 meshes, adding pectase 1200U, cellulase 480U and beta-glucanase 72U into each 100g of fresh sea buckthorn fruit, mixing and carrying out enzymolysis, wherein the enzymolysis temperature is 40-45 ℃, the pH is 5.0-6.0, and the total enzymolysis time is controlled to be 3 hours;
after the enzymolysis is finished, performing ultrasonic treatment for 30min, performing solid-liquid separation, removing residues, and further separating the liquid to obtain an oil phase and a water phase;
filtering and refining the oil phase with ceramic membrane to obtain refined oil, separating and concentrating the water phase with membrane, and drying at no more than 60deg.C to obtain water extract;
mixing the refined oil and the water extract uniformly to obtain the sea buckthorn oily extract.
Example 5:
A preparation method of oleum Hippophae extract comprises the following steps:
Sea buckthorn fresh fruits and water are mixed according to the proportion of 1:3, homogenizing to about 40 meshes, adding pectase 1600U, cellulase 480U and beta-glucanase 65U into each 100g of fresh sea buckthorn fruit, mixing and carrying out enzymolysis, wherein the enzymolysis temperature is 40-45 ℃, the pH is 3.5-4.0, and the total enzymolysis time is controlled to be 4.5 hours;
After the enzymolysis is finished, carrying out ultrasonic treatment on the mixture 40 min, carrying out solid-liquid separation, removing residues, and further separating the liquid to obtain an oil phase and a water phase;
filtering and refining the oil phase with ceramic membrane to obtain refined oil, separating and concentrating the water phase with membrane, and drying at no more than 60deg.C to obtain water extract;
mixing the refined oil and the water extract uniformly to obtain the sea buckthorn oily extract.
Example 6:
A preparation method of oleum Hippophae extract comprises the following steps:
sea buckthorn fresh fruits and water are mixed according to the proportion of 1:4, homogenizing to about 30 meshes, adding pectase 1500U, cellulase 450U and beta-glucanase 60U into each 100g of fresh sea buckthorn fruit, mixing and carrying out enzymolysis, wherein the enzymolysis temperature is 40-45 ℃, the pH is 3.5-4.0, and the total enzymolysis time is controlled to be 4 hours;
After the enzymolysis is finished, carrying out ultrasonic treatment on the mixture 40 min, carrying out solid-liquid separation, removing residues, and further separating the liquid to obtain an oil phase and a water phase;
filtering and refining the oil phase with ceramic membrane to obtain refined oil, separating and concentrating the water phase with membrane, and drying at no more than 60deg.C to obtain water extract;
mixing the refined oil and the water extract uniformly to obtain the sea buckthorn oily extract.
Comparative example 1:
The difference is that the pectase is added in the amount of 800U per 100g fresh sea buckthorn fruit as in example 1.
Comparative example 2:
the difference is that the cellulase is added in the amount of 800U per 100g fresh sea buckthorn fruit as in example 2.
Comparative example 3:
The difference is that no beta-glucanase was added as in example 3.
Comparison of extraction effects for different examples:
The refined oil and the dry water extract of each example were weighed, and the amounts of the refined oil and the dry water extract obtained by extracting each kg of fresh sea buckthorn fruit were calculated, and the results are shown in Table 1.
TABLE 1 product amount (g/kg fresh sea buckthorn fruit) for different examples
| Numbering device | Refined oil/g | Dry water extract/g | Sea buckthorn oil extract/g |
| Example 1 | 1.55 | 8.53 | 10.08 |
| Example 2 | 1.82 | 9.74 | 11.56 |
| Example 3 | 1.49 | 9.83 | 11.32 |
| Example 4 | 1.58 | 8.25 | 9.83 |
| Example 5 | 1.75 | 8.87 | 10.62 |
| Example 6 | 1.72 | 8.65 | 10.37 |
| Comparative example 1 | 1.24 | 6.48 | 7.72 |
| Comparative example 2 | 1.42 | 6.89 | 8.31 |
| Comparative example 3 | 1.55 | 7.73 | 9.28 |
As is clear from Table 1, the examples can obtain more refined sea buckthorn oil and dry aqueous extract than the comparative examples
FIG. 1 is a photograph of refined sea buckthorn oil prepared in example 2, which shows that the refined sea buckthorn oil is clear and transparent and has uniform texture.
Experimental study of the effect of sea buckthorn oil extract on skin trauma
Reference Che Xiping, guo Feng, guan Xiaogong, et al, seabuckthorn seed oil and fruit oil effects on treatment of animal skin scalds, burns, wounds [ J ]. Seabuckthorn, 1999 (4): experimental procedures for effects of 4.2.3 on rat skin wounds, the effects of a portion of seabuckthorn oil extract on skin wounds were tested. The experimental results are shown in table 2.
TABLE 2 repair of skin wounds in rats by Hippophae rhamnoides oily extract
| Grouping | Animal number (only) | Dosage (times/d) | Number of infections | Healing time (d) |
| Physiological saline control group | 10 | 1 | 6 | 25.4±5.2 |
| Example 2 sea buckthorn oil extract | 10 | 1 | 3 | 18.6±3.8 |
| Example 2 sea buckthorn oil extract | 10 | 2 | 2 | 16.6±2.1 |
| Example 5 sea buckthorn oil extract | 10 | 2 | 2 | 18.1±2.9 |
| 1% Amyda sinensis solution | 10 | 1 | 1 | 19.1±3.7 |
The experimental results show that: the sea buckthorn oily extract is used for coating the skin wound of the rat for 1 time or 2 times every day, and the healing time of the wound surface of the animal is obviously shortened compared with that of a saline control group. The sea buckthorn oil extract has the effect of repairing skin wounds. The seabuckthorn oil extract of example 2 has a better effect.
Influence of sea buckthorn oil extract on sensitive muscle and after-sun repair
The sea buckthorn oil extract of different examples is added into the matrix paste according to the addition amount of 5%, so that the use is convenient. The compositions of the skin lotions used for sensitive muscle and after-sun repair are shown in Table 3.
TABLE 3 composition of skin lotions
| Component names or trade names | Dosage/wt.% | Remarks |
| Trisatin (trade name) | 1.00 | Emulsifying agent, available from Fu-Dai trade Co., guangzhou City |
| Monoglyceride (monoglyceride) | 2.00 | |
| Caprylic/capric triglyceride | 3.00 | |
| 350. Silicone oil | 2.50 | |
| No. 26 white oil | 3.00 | |
| 16/18 Alcohol | 2.00 | |
| Vitamin E | 0.50 | |
| EDTA2Na | 0.05 | |
| Clip wave 940 | 0.20 | |
| Glycerol | 6.00 | |
| Hansheng rubber | 0.15 | |
| Para hydroxy acetophenone | 0.60 | |
| Propanol (propanol) | 3.00 | |
| Arginine (Arg) | 0.15 | |
| 1, 2-Hexanediol | 0.60 | |
| Sea buckthorn oil extract | 5.00 | The blank group uses equivalent amount of water instead of |
| Water and its preparation method | To 100% |
Female volunteers with sensitive muscles are selected for 60 people, the ages of which are 18-35 years, and the female volunteers have symptoms of different degrees of damage after sun exposure, such as facial redness, hair scalding, redness and swelling, and the like, and are randomly divided into 5 groups of 12 people; after cleansing the face in the morning and evening, each group of volunteers was smeared with the product of the above example for 3 days.
The post-sun repair effect judgment criteria are as follows:
① The effect is shown: the red and swelling subsides by more than or equal to 60 percent, and the scalding symptoms disappear;
② The method is effective: the red swelling subsides by more than or equal to 30 percent, and the scalding symptoms subside obviously;
③ Invalidation: the red swelling resolved < 30%, or aggravated.
The results are shown in Table 4.
TABLE 4 after-sun repair effects of different lotions
| Group of | Number of apparent effects | Effective number | Number of invalidity |
| Example 1 | 6 | 4 | 2 |
| Example 2 | 8 | 3 | 1 |
| Example 3 | 4 | 6 | 2 |
| Example 4 | 5 | 5 | 2 |
| Blank control | 1 | 7 | 4 |
As can be seen from table 4, the seabuckthorn oil extract of example 2 has a better after-sun restoration effect.
Acne-removing effect of sea buckthorn oily extract
The sea buckthorn oil extract of different examples is added into the matrix paste according to the addition amount of 1.5%, so that the use is convenient. The composition of the repair cream is shown in table 5.
TABLE 5 composition of skin lotions
| Component names or trade names | Dosage/wt.% | Remarks |
| Trisatin (trade name) | 1.00 | Emulsifying agent, available from Fu-Dai trade Co., guangzhou City |
| Monoglyceride (monoglyceride) | 2.00 | |
| Caprylic/capric triglyceride | 3.00 | |
| 350. Silicone oil | 2.50 | |
| No. 26 white oil | 3.00 | |
| 16/18 Alcohol | 2.00 | |
| Vitamin E | 0.50 | |
| EDTA2Na | 0.05 | |
| Clip wave 940 | 0.20 | |
| Glycerol | 6.00 | |
| Hansheng rubber | 0.15 | |
| Para hydroxy acetophenone | 0.60 | |
| Propanol (propanol) | 3.00 | |
| Arginine (Arg) | 0.15 | |
| 1, 2-Hexanediol | 0.60 | |
| Sea buckthorn oil extract | 1.50 | The blank group uses equivalent amount of water instead of |
| Sophora flavescens extract | 2.00 | |
| Aloe extract | 1.50 | |
| Water and its preparation method | To 100% |
Female volunteers 60 with different degrees of acne on the face are selected, the ages of the female volunteers are 21-33 years, the female volunteers are randomly divided into 5 groups, each group of the female volunteers is 12 people, after face cleaning in the morning and evening, the products are respectively smeared on the volunteers in each group, and the female volunteers are continuously used for 15 days.
The acne removal effect judgment criteria are as follows:
① The effect is shown: the fading rate of facial acne is more than or equal to 60 percent, and a small amount of pock marks remain;
② The method is effective: the resolution rate of facial acne is more than or equal to 30%;
③ Invalidation: the resolution rate of facial acne is less than 30 percent.
The results are shown in Table 6.
TABLE 6 acne-removing effect of different repair creams
| Group of | Number of apparent effects | Effective number | Number of invalidity |
| Example 1 | 7 | 3 | 2 |
| Example 2 | 9 | 3 | 0 |
| Example 3 | 6 | 5 | 1 |
| Example 4 | 6 | 4 | 2 |
| Blank control | 5 | 4 | 3 |
As can be seen from table 6, the seabuckthorn oil extract of example 2 has better acne-removing effect.
The above description of the present invention is further illustrated in detail and should not be taken as limiting the practice of the present invention. It is within the scope of the present invention for those skilled in the art to make simple deductions or substitutions without departing from the concept of the present invention.
Claims (10)
1. A preparation method of oleum Hippophae extract comprises the following steps:
homogenizing fresh fructus Hippophae, adding pectase, cellulase and beta-glucanase, and performing enzymolysis;
After enzymolysis is completed, carrying out ultrasonic treatment, carrying out solid-liquid separation, removing slag, and further separating liquid to obtain an oil phase and a water phase;
Filtering the oil phase to obtain refined oil, concentrating the water phase, and drying to obtain water extract;
mixing the refined oil and the water extract uniformly to obtain the sea buckthorn oily extract.
2. The preparation method according to claim 1, wherein the mass mixing ratio of fresh sea buckthorn fruit to water is 1: (0.5-5).
3. The method of claim 1, wherein the pectase: cellulase: beta-glucanase = 100U: (20-40) U: (3-6) U.
4. A method of preparing as claimed in claim 3 wherein the mixing ratio of fresh seabuckthorn to pectase is 1 kg for fresh seabuckthorn: (10000-20000) U.
5. The method according to claim 1, wherein the time for the enzymolysis treatment is 3 to 5 hours.
6. The method according to claim 1, wherein the time of the ultrasonic treatment is 30 to 60 min.
7. The process of claim 1, wherein the aqueous phase is concentrated to dryness at a temperature not exceeding 60 ℃.
8. The method according to claim 1, wherein the oil phase is purified by filtration through a ceramic membrane, and the aqueous phase is concentrated by membrane separation and then dried.
9. The seabuckthorn oil extract prepared by the preparation method according to any one of claims 1 to 8.
10. A composition comprising the seabuckthorn oil extract of claim 9.
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