CN118104667A - Binary insecticidal synergistic composition and application thereof - Google Patents

Binary insecticidal synergistic composition and application thereof Download PDF

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Publication number
CN118104667A
CN118104667A CN202211511894.1A CN202211511894A CN118104667A CN 118104667 A CN118104667 A CN 118104667A CN 202211511894 A CN202211511894 A CN 202211511894A CN 118104667 A CN118104667 A CN 118104667A
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compound
active component
spinetoram
emamectin benzoate
emamectin
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常秀辉
范晓溪
孙俊
宋玉泉
刘少武
张俊龙
呼健洋
刘艳
徐靖博
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Jiangsu Youjia Plant Protection Co ltd
Jiangsu Yangnong Chemical Co Ltd
Shenyang Sinochem Agrochemicals R&D Co Ltd
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Jiangsu Youjia Plant Protection Co ltd
Jiangsu Yangnong Chemical Co Ltd
Shenyang Sinochem Agrochemicals R&D Co Ltd
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Priority to CN202211511894.1A priority Critical patent/CN118104667A/en
Priority to PCT/CN2023/128516 priority patent/WO2024114260A1/en
Publication of CN118104667A publication Critical patent/CN118104667A/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/24—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with two or more hetero atoms
    • A01N43/26—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with two or more hetero atoms five-membered rings
    • A01N43/28—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with two or more hetero atoms five-membered rings with two hetero atoms in positions 1,3
    • A01N43/30—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with two or more hetero atoms five-membered rings with two hetero atoms in positions 1,3 with two oxygen atoms in positions 1,3, condensed with a carbocyclic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P5/00—Nematocides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • A01P7/02—Acaricides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • A01P7/04—Insecticides

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Abstract

The invention discloses an insecticidal synergistic composition containing piperic acid derivative compounds and application thereof, wherein the composition consists of two active ingredients, namely a compound A and a compound B, and the component B is selected from any one or two of macrolide insecticide and acaricide; the macrolide insecticide comprises avermectin, spinosad, spinetoram, emamectin benzoate, liuyangmycin, emamectin, ivermectin, milbemycin, mi Beier mycin and the like; the compound I-72 has the following structure:

Description

Binary insecticidal synergistic composition and application thereof
Technical Field
The invention belongs to the technical field of pesticide application, and in particular relates to a binary insecticidal synergistic composition containing piperic acid derivative compounds and macrolide internal insecticidal and acaricidal agents and application thereof.
Background
In agricultural production practice, the application of chemical agents is still the most effective means for controlling plant diseases and insect pests, however, the single-component pesticide varieties often have certain limitations in pest control, such as narrow insecticidal spectrum, higher cost, short duration, extremely easy occurrence of resistance when continuously used pests, and the like. The two pesticides with different action mechanisms are mixed into mixed components according to a certain proportion, so that the defects can be overcome, and the effects of improving the efficacy, expanding the insecticidal spectrum, slowing down the generation of resistance and the like are achieved.
The macrolide pesticides such as abamectin, spinosad, emamectin benzoate and the like are novel biological source pesticides, have the characteristics of high insecticidal activity, wide insecticidal spectrum, safety to crops, no pollution and the like, and become the most popular and strongly competitive products in the current pesticide market. However, with the wide application of avermectin in pest and mite control, the problem of resistance is increasingly serious. The literature reports that the plutella xylostella and the tetranychus urticae have serious drug resistance to abamectin in partial areas, and once the resistance is more difficult to recover, reasonable blending becomes one of effective means for prolonging the service life of the variety.
The Chinese patent CN112457288 discloses a piperic acid derivative and application thereof, wherein the compound I-72 has contact killing and stomach poisoning activities and good conduction activities, and can be used for preventing and controlling various pests such as lepidoptera, hemiptera, thysanoptera, coleoptera and the like. The compound has novel structure, wide insecticidal spectrum, high activity and no cross resistance with other pesticides, and is expected to become an environment-friendly medicament for controlling resistant pests.
The mixed use of pesticides with different action mechanisms can generate a plurality of additional advantages and effects, can overcome the defects of single use of pesticides, improve the pesticide effect and slow down the generation of resistance, and more importantly, the reasonable mixing of pesticides can prolong the service life of new varieties in the market and reduce the research and development cost of new pesticides. By literature search, the technical solution of the binary pesticide composition containing the compound I-72 is not specifically disclosed.
Disclosure of Invention
The invention aims to develop a synergistic binary pesticide composition containing a compound I-72 and application thereof in effectively preventing and controlling lepidoptera and diptera pests and in preventing and controlling resistant pests and mites.
The technical scheme adopted by the invention for achieving the purpose is as follows:
A binary insecticidal synergistic composition consists of A, B active ingredients, wherein the active ingredient A is selected from a compound I-72, and the active ingredient B is selected from macrolides insecticidal and acaricidal agents; the weight part ratio of the two active components is 100:1-1:100;
The compound I-72 has the following structure:
the macrolide pesticide is selected from avermectin, spinetoram, spinosad, emamectin benzoate (simply called emamectin benzoate), liuyanmycin, ivermectin, emamectin, milbemycin, mi Beier mycin, doramectin, acetaminophen, moxidectin, selamectin, alkenyl emamectin, aureomycin, nikkomycins or Milbemycin Oxime.
Preferably, the active component A in the composition is selected from a compound I-72, and the active component B is selected from abamectin, spinetoram or emamectin benzoate; the weight ratio of the two active components is 50:1-1:50.
Further preferably, the active component A in the composition is selected from the group consisting of compound I-72, and the active component B is selected from the group consisting of avermectin, spinetoram or emamectin benzoate; the weight ratio of the two active components is 50:1-1:20.
Still further preferably, the active component A in the compound is selected from the group consisting of compound I-72 and the active component B is selected from the group consisting of avermectin, spinetoram or emamectin benzoate; the weight part ratio of the two active components is 20:1 to 1:20.
Or, the active component A in the compound is selected from the compound I-72, and the active component B is selected from abamectin, spinetoram or emamectin benzoate; the weight part ratio of the two active components is 10:1 to 1: 10.
The binary pesticide composition can be used for controlling pests, mites and parasitic nematodes in agriculture, forestry, fruits, vegetables and tea crops.
The weight part ratio of the two active components in the binary pesticide composition is 100:1-1:100, preferably 50:1 to 1:20, more preferably 20:1 to 1:10.
Indoor biological activity measurement shows that the binary pesticide composition has synergistic effect. For example, compound I-72 is mixed with abamectin, spinosad, spinetoram at 50:1 to 1: the compound I-72 and emamectin benzoate have good synergism to lepidoptera target corn borer within the range of 50, and the compound I-72 and emamectin benzoate are shown in the range of 50:1 to 1: the synergistic effect on corn borers is shown in the range of 10; compound I-72 and abamectin, spinetoram at 10:1 to 1: the synergistic effect on the Chilo suppressalis is achieved within the proportion range of 10; compound I-72 with emamectin benzoate at 10:1 to 1: the synergistic effect on the asparagus caterpillar is achieved within the proportion range of 10; compound I-72 with spinosad at 10:1 to 1: the synergistic effect on spodoptera frugiperda is achieved within the proportion range of 10; the compound I-72 and spinetoram are mixed in a ratio of 10:1-1:10, and have obvious synergism on 2-year nymphs of frankliniella occidentalis; the compound I-72 and emamectin benzoate are mixed at a ratio of 10:1-1:10 to have obvious synergism on bemisia tabaci adults; the compound I-72 and the abamectin are mixed at a ratio of 10:1-1:10, so that the synergistic effect on the bemisia tabaci adults is obvious.
The insecticidal and acaricidal compositions according to the invention are suitable for controlling pests, mites, and parasitic nematodes occurring in agriculture, horticulture, forests and ornamental plants or on tissues and organs of such plants, for example fruits, flowers, leaves, stems, tubers or roots.
The compositions of the present invention are effective against susceptible and resistant species and all or individual developmental stages of pests including, but not limited to, lepidoptera, thysanoptera, coleoptera, hemiptera, diptera, homoptera, orthoptera, isoptera, hymenoptera, and mites or parasitic nematodes.
Preferably, the lepidopteran pest, such as chilo suppressalis, cnaphalocrocis medinalis, tryporyza incertulas, borer, silk worm, pod borer, sugarcane borer, armyworm, corn borer, athetis lepigone, cotton bollworm, beet armyworm, prodenia litura, cabbage looper, fall webworm, cutworm, geometrid, peach fruit borer, pear fruit borer, soybean fruit borer, spodoptera frugiperda, plutella xylostella, cabbage caterpillar, tobacco budworm, tobacco leaf looper.
Preferably, the thysanoptera pests, such as frankliniella occidentalis, palmtop thrips, shallot thrips, rice thrips, large Jiang Jima, mango thrips.
Preferably, the homopteran pest, such as bemisia tabaci, trialeurodes vaporariorum, myzus persicae, myzus gossypii, aphis medicata, aphis pomace, psyllid, brown planthopper, and Laodelphax striatellus.
Preferably, the coleopteran pest, such as flea beetle, coccinella twenty-eight, potato beetle, wireworms, grubs.
Preferably, the parasitic nematodes, such as root knot nematodes, soybean cyst nematodes, sweet potato stem nematodes, aphelenchus xylophilus, and pine wood nematodes.
The compositions of the present invention are suitable for, but not limited to, the following crops or plants:
Cereal crops, such as wheat, barley, rye, oats, rice, maize, sorghum, millet, etc.; tuber crops: including sweet potato, yam, potato, etc.; legume crops: including soybean, broad bean, pea, mung bean, etc.; fiber crops: including cotton, jute, kenaf, flax, sisal, and the like; oil crops: including oilseed, vines, mustards, peanuts, flax, sunflowers and the like; sugar crops: including sugarcane, beet, etc.; beverage crops: including tea, coffee, cocoa, etc.; dye crops: including indigo, saffron, etc.; spice crops: comprises lemongrass, peppermint and rose; feed crops: comprises alfalfa, sweet clover, milk vetch, vetch and the like; forage crops including ryegrass, oat grass, sudan grass, and the like; green manure crops such as vetch, milk vetch, alfalfa, sweet clover, tamarix, sesbania, amorpha fruticosa, etc.; fruits such as apples, pears, plums, peaches, almonds, cherries, oranges, mangos, litchis, bananas, grapes; or berries, such as strawberry, raspberry or blackberry mustard, poppy, olive, coconut, castor, etc.; vegetables such as cucumber, luffa, watermelon, melon, pumpkin, melon, spinach, celery, cabbage, cauliflower, cucurbit, capsicum, eggplant, tomato, onion, ginger, garlic, leek, lettuce, beans, cowpea, radish, carrot, yam, etc.; the compositions of the invention may also be used on any ornamental plant, including flowers, shrubs, broadleaf trees and evergreen trees, for example, violet, begonia, dahlia, african aster, hydrangea, verbena, rose, kalanchoe, poinsettia, aster, cornflower, golden aster, delphinium, monopetiole, fluccous, rhodiola, sedum, petunia, viola, impatiens, geranium, chrysanthemum, buttercup, majoram, red, sage, wild banksia, rosemary, sage, san john's woad, peppermint.
Compared with the prior art, the composition has the following advantages: the composition has obvious synergistic effect, and improves the control effect on pests; because the compound I-72 has novel action mechanism and no interactive resistance with the existing pesticide, the composition can be used for treating resistant pests, and the application of the composition not only expands the insecticidal spectrum of the compound I-72, but also delays the generation and development of the drug resistance of the pests. The invention mixes the active component A with the pesticide to generate a plurality of additional advantages and effects, can overcome the defects of single use of the pesticide, improves the pesticide effect, slows down the generation of resistance, and more importantly, prolongs the service life of new varieties in the market by reasonably mixing the pesticide, and reduces the research and development cost of the new pesticide.
Detailed description of the preferred embodiments
The following specific examples serve to further illustrate the invention in detail, but the invention is by no means limited to these examples.
The active component A in the examples is compound I-72 prepared by the description in CN112457288 patent, and the content is 97%; the active component B and other raw materials are all commercial products. The percentages or the proportions of the components are calculated by weight. The active components in each formulation are calculated as effective content.
The structural formula of the compound I-72 is as follows:
Example 1 synergistic effect of compositions containing Compound I-72 on corn borer
Test subject: corn borer (Pyrausta nubilalis Hubern), 3-instar larvae, and indoor feeding sensitive strain.
Test conditions: temperature: 24-26 ℃, humidity: RH 60%, illumination: l: d=14:10
The preparation method comprises the following steps: the method comprises the steps of respectively and accurately weighing the tested medicines by an electronic analytical balance, respectively adding proper amounts of solvents into the two raw medicines to be completely dissolved, preparing mother solutions with required concentration by water containing 0.1% of Tween 80, mixing the mother solutions with the two effective components according to a certain proportion to prepare mixed solutions, and respectively diluting the mixed solutions into series of liquid medicines with certain concentration gradients according to test design doses.
The test method comprises the following steps: adopts an artificial feed medicine membrane method. A standard clean 24-hole culture plate is selected, 1ml of artificial feed is added into each hole, after cooling and solidification, a continuous sampler is used for adding a certain amount of liquid medicine into the culture plate, and natural drying in the shade is carried out. Standard test insects were accessed, 1 head per well, 48 heads total. And (3) placing the treated test insects in an observation chamber under a certain condition, periodically observing the reaction condition of the test insects, and investigating the number of dead and alive insects after 72 hours.
The evaluation method adopts the Bliss method. The method is suitable for qualitative screening of insecticidal compositions. The theoretical mortality rate P of the pesticide mixture according to its proposed independent combined action concept, as suggested by Bliss, can be calculated by:
P＝Pm+Pn(1-Pm)
P m is the mortality (%) of the first active component target at a concentration of m; p n is the mortality (%) of the target when the second active component is used at a concentration of n.
If the actual mortality of the target is greater than the theoretical mortality P after the two active components are mixed at a certain concentration, the two active components are judged to have synergistic effect when mixed at a set concentration, and otherwise, antagonistic effect is judged.
The test results are shown in Table 1. From the table, the compound I-72 and abamectin, spinosad and spinetoram are mixed to show synergy between 50:1 and 1:50; the synergistic effect is achieved when the compound is mixed with emamectin benzoate in a ratio of 50:1-1:10.
TABLE 1 synergistic effect of compositions containing Compound I-72 on corn borer 3-instar larvae
Example 2 indoor Combined toxicity determination of Rice stem borer Using Compound I-72 in combination with Avermectin
Test target: chilo suppressalis Chilo suppressalis (Walker), 2-year larva, insect source from Jiangxi Nanchang, indoor continuous culture for 11 th generation, and high drug resistance level to amide drugs such as chlorantraniliprole.
Test conditions: temperature: 25-27 ℃, humidity: RH 60%, illumination: l: d=14:10
The preparation method comprises the following steps: the method comprises the steps of respectively and accurately weighing the tested medicines by an electronic analytical balance, respectively adding proper amounts of solvents into the two raw medicines to be completely dissolved, preparing mother solutions with required concentration by water containing 0.1% of Tween 80, mixing the mother solutions with the two effective components according to a certain proportion to prepare mixed solutions, and respectively diluting the mixed solutions into series of liquid medicines with certain concentration gradients according to test design doses.
The test method comprises the following steps: spray method is adopted. Selecting rice stems with consistent length and thickness, cutting the same length, uniformly spraying according to the sequence from low dose to high dose according to test design, placing 1.5mL of liquid medicine in a culture dish with the diameter of 6cm in which filter paper is placed, naturally drying in the shade, inoculating regular healthy insects, repeating for 4 times in each treatment of 10 heads, and additionally arranging blank control. The number of dead and alive insects was investigated after 72 hours. The corrected mortality was calculated using the abbott formula, and the toxicity LC 50 values for the single and the test mix were calculated using statistical analysis using DPS data processing software.
The evaluation method comprises the following steps: and (3) solving the co-toxicity coefficient of the mixed ingredients by adopting a Sun cloud Peel (Sun Y-P) method, and evaluating the combined action type of each mixed ingredient.
The co-toxicity coefficient (CTC value) of the blend was calculated according to the following formula:
Wherein: the ATI-mixed agent actually measures the toxicity index; LC 50 of S-standard agent; LC 50 of M-mix.
TTI＝TIA×PA+TIB×PB
Wherein: TTI-mixed agent theoretical virulence index; TIA-A medicament toxicity index; the percentage of the PA-A agent in the mixture; TIB-B agent virulence index; the PB-B medicament comprises the following components in percentage by weight.
Wherein: CTC-co-toxicity coefficient; the ATI-mixed agent actually measures the toxicity index; TTI-blend theoretical virulence index.
CTC-Co-toxicity coefficient
ATI-actual measured toxicity index of mixture
TTI-theoretical toxicity index of Mixed Agents
The co-toxicity coefficient (CTC) of the compound in the formula is more than or equal to 120, and the compound shows synergistic effect; ctc.ltoreq.80 shows antagonism; 80. < CTC < 120 shows additive effect.
It can be seen from the table (table 2) that the compound I-72 and abamectin are compounded to have a synergistic effect against chilo suppressalis at a ratio of 10:1-1:10.
Table 2 results of indoor Combined toxicity determination of Chilo suppressalis by mixing Compound I-72 with Avermectin
EXAMPLE 3 use of Compound I-72 in combination with spinetoram for indoor Activity determination of Chilo suppressalis
The test subjects, test conditions, chemical formulation, test methods and evaluation methods were the same as in example 2.
As can be seen from the table (Table 3), the compound I-72 and spinetoram were compounded at a ratio of 10:1 to 1:10 to show a synergistic effect against chilo suppressalis.
Table 3 results of indoor Combined toxicity determination of Chilo suppressalis by Mixed use of Compound I-72 and spinetoram
EXAMPLE 4 indoor Activity assay of Compound I-72 in combination with emamectin benzoate on Spodoptera exigua
Test subject: beet armyworm (Spodoptera exigua H u bner), 3-instar larva, indoor feeding sensitive strain
The test method comprises the following steps: the dipping method is adopted. Taking cabbage leaves cultivated in a greenhouse, removing a surface waxy layer, preparing leaf discs with the diameter of 3cm by using a puncher, immersing the leaf discs in the prepared liquid medicine for 10s, putting the leaf discs in a culture dish with the diameter of 9cm, naturally drying in the shade, inoculating standard insects to be tested, inoculating 10 heads of insects in each dish, and repeating for 3 times. And (3) placing the treated test insects in an observation chamber under a certain condition, periodically observing the reaction condition of the test insects, and investigating the number of dead and alive insects after 72 hours.
Test conditions, preparation of a drug solution, and evaluation methods were the same as in example 2.
As can be seen from the table (Table 4), the compound I-72 and emamectin benzoate show obvious synergism to the 3-instar larvae of asparagus caterpillar when mixed at a ratio of 10:1-1:10.
Table 4 results of indoor Combined toxicity measurements of Spodoptera exigua by mixing Compound I-72 with emamectin benzoate
EXAMPLE 5 indoor Activity assay of Compound I-72 in combination with spinosad on Spodoptera frugiperda
Test subject: spodoptera frugiperda (Spodoptera frugiperda), 3-instar larvae, sensitive strain fed indoors
The test method comprises the following steps: the dipping method is adopted. Cutting corn leaves cultivated in a greenhouse into 5cm long leaf segments, soaking the corn leaves in the prepared liquid medicine for 10 seconds, naturally drying in the shade, cutting the corn leaves into small pieces with the length of 1.5cm and the length of 1.5cm, placing the small pieces in a 24-hole culture plate, inoculating standard tested insects, and treating 48 insects each time. And (3) placing the treated test insects in an observation chamber under a certain condition, periodically observing the reaction condition of the test insects, and investigating the number of dead and alive insects after 72 hours.
Test conditions, preparation of a drug solution, and evaluation methods were the same as in example 2.
As can be seen from the table (Table 5), the compound I-72 and spinosad combination showed significant synergy against spodoptera frugiperda 3-instar larvae at a ratio of 10:1 to 1:10.
TABLE 5 determination of indoor combined toxicity of Compound I-72 and spinosad on Spodoptera frugiperda
EXAMPLE 6 use of Compound I-72 in combination with spinetoram for indoor Activity determination against Frankliniella occidentalis
Test subject: frankliniella occidentalis FRANKLINIELLA OCCIDENTALIS, 2-year nymphs, and sensitive strains are fed indoors.
The test method comprises the following steps: selecting green bean leaves cultivated in a greenhouse by adopting a spraying method, wrapping leaf stalks by wet de-esterified cotton and a sealing film, uniformly spraying according to the sequence from low dose to high dose according to test design, placing the green bean leaves in a culture dish with the diameter of 6cm and filter paper, naturally drying in the shade, inoculating tested insects, repeating for 3 times every 20 heads, and additionally setting a blank control. The number of dead and alive insects was investigated after 48 hours. The corrected mortality was calculated using the abbott formula, and the toxicity LC 50 values for the single and the test mix were calculated using statistical analysis using DPS data processing software.
Test conditions, preparation of a drug solution, and evaluation methods were the same as in example 2.
From the table, it can be seen (Table 6) that the compound I-72 and spinetoram have obvious synergism on the 2-year nymphs of frankliniella occidentalis when mixed in a ratio of 10:1-1:10.
Table 6 results of indoor combined toxicity measurements on frankliniella occidentalis using a combination of compound I-72 and spinetoram
Example 7 compound I-72 in combination with emamectin benzoate indoor activity assay for bemisia tabaci adults test subjects: bemisia tabaci Bemisiatabaci (Gennadius), adults, and indoor feeding sensitive strains.
The test method comprises the following steps: adopting a spraying method, selecting green bean seedlings cultivated in a greenhouse, uniformly spraying according to the sequence from low dose to high dose of test design, naturally drying in the shade, covering a self-made glass cover, inoculating tested insects, repeating for 3 times every 20-30 heads, and additionally setting a blank control. The number of dead and alive insects was investigated after 72 hours. The corrected mortality was calculated using the abbott formula, and the toxicity LC 50 values for the single and the test mix were calculated using statistical analysis using DPS data processing software.
Test conditions, preparation of a drug solution, and evaluation methods were the same as in example 2.
As can be seen from the table (Table 7), the compound I-72 and emamectin benzoate have obvious synergism on the bemisia tabaci adults when mixed at a ratio of 10:1-1:10.
Table 7 results of indoor Combined toxicity measurements on Bemisia tabaci by mixing Compound I-72 with emamectin benzoate
EXAMPLE 8 indoor Activity assay of Compound I-72 in combination with Avermectin on Bemisia tabaci adults
Test subjects, test conditions, chemical formulation, and evaluation methods were the same as in example 2.
As can be seen from the table (Table 8), the compound I-72 and abamectin have obvious synergistic effect on bemisia tabaci adults when mixed in a ratio of 10:1-1:10.
Table 8 results of indoor Combined toxicity measurements on Bemisia tabaci by mixing Compound I-72 with Avermectin

Claims (7)

1. A binary insecticidal synergistic composition is characterized in that: the composition consists of A, B active components, wherein the active component A is selected from a compound I-72, and the active component B is selected from macrolides insecticidal and acaricidal agents; the weight part ratio of the two active components is 100:1-1:100;
The compound I-72 has the following structure:
2. A binary insecticidal synergistic composition as claimed in claim 1, characterised in that: the macrolide pesticide is selected from avermectin, spinetoram, spinosad, emamectin benzoate (simply called emamectin benzoate), liuyanmycin, ivermectin, emamectin, milbemycin, mi Beier mycin, doramectin, acetaminophen, moxidectin, selamectin, alkenyl emamectin, aureomycin, nikkomycins or Milbemycin Oxime.
3. A pesticidal composition according to claim 1 or 2, wherein: the active component A is selected from a compound I-72, and the active component B is selected from abamectin, spinetoram or emamectin benzoate; the weight ratio of the two active components is 50:1-1:50.
4. A pesticidal composition according to claim 3, wherein: the active component A is selected from a compound I-72, and the active component B is selected from abamectin, spinetoram or emamectin benzoate; the weight ratio of the two active components is 50:1-1:20.
5. A composition according to claim 3, characterized in that: the active component A is selected from a compound I-72, and the active component B is selected from abamectin, spinetoram or emamectin benzoate; the weight part ratio of the two active components is 20:1 to 1:20.
6. A composition according to claim 3, characterized in that: the active component A is selected from a compound I-72, and the active component B is selected from abamectin, spinetoram or emamectin benzoate; the weight part ratio of the two active components is 10:1 to 1:10.
7. Use of a binary pesticide composition according to claim 1 for controlling pests, mites and parasitic nematodes in agricultural, forestry, fruit, vegetable and tea crops.
CN202211511894.1A 2022-11-29 2022-11-29 Binary insecticidal synergistic composition and application thereof Pending CN118104667A (en)

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