CN118084565A - Plant growth regulator for promoting rice fruiting and grouting and use method and application thereof - Google Patents

Plant growth regulator for promoting rice fruiting and grouting and use method and application thereof Download PDF

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Publication number
CN118084565A
CN118084565A CN202410208760.5A CN202410208760A CN118084565A CN 118084565 A CN118084565 A CN 118084565A CN 202410208760 A CN202410208760 A CN 202410208760A CN 118084565 A CN118084565 A CN 118084565A
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rice
plant growth
growth regulator
grouting
leucine
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陈婷婷
王莹
符冠富
奉保华
王文婷
陶龙兴
符卫蒙
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China National Rice Research Institute
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China National Rice Research Institute
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/10Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/24Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing the groups, or; Thio analogues thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00Plant growth regulators
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00Fungicides
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C11/00Other nitrogenous fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a plant growth regulator for promoting rice fruiting and grouting, and a use method and application thereof, wherein the plant growth regulator comprises the active ingredients of naphthylacetic acid, bactericide and leucine, the naphthylacetic acid is 10-50 mu mol/L, the bactericide is 0.1-0.5% in volume percentage concentration, and the leucine is 0.1-0.5% in mass percentage concentration. Wherein the bactericide is oryzanol. When the plant growth regulator is used, the mixed aqueous solution of the plant growth regulator is used for spraying rice leaves and rice ears, and is respectively sprayed in the flowering period and the middle stage of grain grouting of rice. The plant growth regulator for promoting rice seed setting and grouting is used for promoting rice seed setting and grouting, and can improve seed setting rate and grain weight and increase rice yield.

Description

Plant growth regulator for promoting rice fruiting and grouting and use method and application thereof
Technical Field
The invention relates to the field of plant growth regulators, in particular to a plant growth regulator for promoting rice fruiting and grouting, and a use method and application thereof.
Background
The fruiting rate of rice is one of the important factors of yield, and is mainly determined by flowering fertilization and grain filling. In the flowering and fertilization process of rice, pollen grains are firstly transferred to a pistil column head through anther cracking of stamen, then pollen tubes are sent out by pollen grains germination to extend into the column head, the pollen tubes pass through the anther to reach ovaries and ovules, and a pair of carried sperm cells are sent into embryo sacs to fertilize with egg cells and central cells respectively. The fertilized ovary is continuously expanded and enriched, endosperm cells are continuously proliferated, accumulated and stored with starch, and the seed grains are filled and developed into caryopsis. The fertilization quality of the former in flowering determines the quantity of empty grains on the small spikes, and the filling degree of the latter grains influences the quantity of shrunken grains on one hand and the grain weight on the other hand, and finally determines the yield of the rice.
The fertilization setting period is a period in which the rice is very sensitive to environmental factors such as moisture, temperature and the like, and adverse environmental factors influence the setting rate of small spikes by influencing glume cracking, pollen grain fertility, germination and elongation of pollen tubes and the like; the environmental factors in the grouting period influence the grain grouting filling degree mainly by influencing the proliferation rate of endosperm cells, so that the yield is determined, and the quality of rice is influenced by influencing the stacking and arranging process of starch particles. For example, drought stress during flowering and fruiting period can delay heading, reduce germination rate of stigma pollen grains, increase degradation number of glume flowers, reduce grain number of ears, reduce grain weight and deteriorate rice quality. In the high-temperature weather with the highest air temperature of 35 ℃ or above in the flowering period, the seed setting rate of the rice variety is reduced to about 55 percent, and the yield is reduced by 160 kg/mu. The main mechanism comprises high temperature inhibition of anther cracking, stigma pollen germination and elongation of pollen tubes in pistil tissues, thus leading to small ear abortion, increased empty grains and reduced setting rate.
It is found that the application of nitrogen, especially in flowering and fruiting period, can improve the nutritional status of rice in late growth period, delay the aging of functional leaves, increase the seed storage capacity and activity, coordinate the balance among source, storage and flow, increase or decrease the proportion of photosynthetic product occupied by the seed storage capacity, and increase the physiological activity of seed grouting. Some researches prove that moderate soil drought after the heading and flowering of the rice is favorable for improving photosynthesis of sword leaves, increasing accumulation of assimilates, and influencing the activity of key enzymes of a starch synthesis pathway by regulating balance of certain hormones in plants so as to promote filling of seeds with grouting materials. Compared with the regulation and control of the traditional water and fertilizer and other cultivation modes, the chemical regulation and control substances are sprayed, so that the chemical regulation and control substances have timeliness and high efficiency, and are also one of the most effective measures for regulating the physiological activity of the rice fruiting grouting and coping with unpredictable environmental stress. Therefore, how to provide a plant growth regulator which synergistically promotes rice setting and grouting is a great goal of improvement in the current industry.
Disclosure of Invention
The invention aims to provide a plant growth regulator capable of synergistically promoting rice setting grouting, and a use method and application thereof.
In order to solve the technical problems, the invention adopts the following technical scheme:
On one hand, the invention provides a plant growth regulator for promoting rice fruiting and grouting, which comprises the active ingredients of Naphthalene Acetic Acid (NAA), bactericide and Leucine (LEC), wherein the naphthalene acetic acid is 10-50 mu mol/L, the bactericide is 0.1-0.5% in volume percentage concentration, and the leucine is 0.1-0.5% in mass percentage concentration according to the use concentration.
Further, the bactericide is oryzanol.
Further, the content of the active ingredients in the rice whey is 9% pyraclostrobin.
Further, the naphthalene acetic acid is 10-20 mu mol/L, the volume percentage concentration of the bactericide is 0.1-0.22%, and the mass percentage concentration of the leucine is 0.1-0.2%.
Further, the naphthalene acetic acid is 10 mu mol/L, the volume percentage concentration of the bactericide is 0.1%, and the mass percentage concentration of the leucine is 0.1%;
Or, the naphthalene acetic acid is 20 mu mol/L, the volume percentage concentration of the bactericide is 0.2-0.22%, and the mass percentage concentration of the leucine is 0.2%.
Further, the plant growth regulator also contains auxiliary agents so as to be prepared into a formulation suitable for agriculture.
In a second aspect, the invention also provides a use method of the plant growth regulator for promoting rice seed setting and grouting, wherein the mixed aqueous solution of the plant growth regulator is used for spraying on rice leaves and rice ears, and is sprayed in a flowering period and a middle stage of grain grouting of rice respectively.
In a third aspect, the invention also provides an application of the plant growth regulator for promoting rice seed setting and grouting, which is used for promoting rice seed setting and grouting, improving seed setting rate and grain weight and increasing rice yield.
By adopting the technical scheme, the invention has at least the following beneficial effects:
(1) According to the invention, research shows that the ternary compound combination of the plant growth regulator naphthalene acetic acid, the bactericide oryza sativa serum and leucine has obvious influence on the grain grouting growth in the flowering period and the later heading period of rice, the optimal application concentration range of the compound combination is screened, the grain grouting of the rice under the concentration combination is obviously improved, the material transfer rate is obviously improved, the fruiting rate and grain weight are obviously increased, and the yield is obviously increased.
(2) The plant growth regulator of the invention can be widely applied to rice production, promote the seed setting and grouting, increase the seed setting rate and grain weight and improve the yield. The significance is that the method not only can be applied to varieties which are applied in large area in production and used for improving the seed setting grouting, but also has important reference value for hybrid seed production with lower seed setting rate.
Detailed Description
The plant growth regulator for promoting rice seed setting and grouting is mainly used for promoting rice seed setting and grouting, improving seed setting rate and grain weight and increasing rice yield. The active ingredients of the composition are naphthalene acetic acid, bactericide and leucine, and the composition belongs to a ternary compound composition, wherein the naphthalene acetic acid is a plant growth regulator, the bactericide mainly plays a role in sterilization, and the leucine is an amino acid nutrient. Calculated according to the use concentration, wherein, the naphthylacetic acid is 10-50 mu mol/L, the volume percentage concentration of the bactericide is 0.1-0.5%, and the mass percentage concentration of the leucine is 0.1-0.5%. Preferably, the bactericide is selected from rice whey (Bass China Co., ltd.) with an active ingredient content of 9% pyraclostrobin. Further preferably, the naphthalene acetic acid is 10-20 mu mol/L, the bactericide is 0.1-0.22% in volume percentage concentration, and the leucine is 0.1-0.2% in mass percentage concentration.
The concentration of the configuration in use can be referred to as follows:
naphthalene acetic acid: 3.724mg in 1L water (1000 g water), i.e. at a concentration of 20. Mu. Mol/L;
Rice serum (the active ingredient is pyraclostrobin with the content of 9 percent): 0.22mL of the mixture is dissolved in 100mL of water, namely, the volume percentage concentration is 0.22%;
Leucine: 0.2g is dissolved in 100mL of water (100 g of water), i.e., the mass percent concentration is 0.2%.
The plant growth regulator can be prepared into a dosage form suitable for agriculture by adding auxiliary agents besides active ingredients. Such as common wettable powder, water dispersible granules, microemulsion, emulsion in water, emulsifiable concentrate, suspending agent or granules, and the like. The present embodiment is preferably a water-dispersible granule.
When the plant growth regulator is used in a field, the mixed aqueous solution of the plant growth regulator is used for spraying rice leaves and rice ears, and the plant growth regulator is used in a spraying mode in a rice flowering period and a grain grouting medium period (20 days after flowering) respectively; the spraying liquid medicine amount per mu is 30 liters, and the leaf surface and the rice spike are uniformly sprayed.
The following is a detailed description of the embodiments:
2021-2022, zhejiang Fuyang China rice research institute test field. The rice varieties to be tested are large-spike indica-japonica hybrid rice Chun you 927 and Chun you 7860 with higher grain number per spike. Spraying for 2 times in the flowering period and the grouting medium period respectively, wherein the concentration of each component is 20 mu mol/L of naphthylacetic acid, 0.22% of oryza sativa L (Pasteff) and 0.2% of leucine.
The tested variety is flowering in 9 months and 5-10 days, after the combination of naphthalene acetic acid, oryza sativa serum, leucine and its compound agent is sprayed in flowering period and grain grouting medium 9 months and 25-30 days, the effect of the tested variety on physiological indexes of rice leaves is analyzed by sampling every other day, and the yield and composition change of the tested variety under the treatment of each agent are measured in the harvest period.
Table 1 shows the variation of the yield of Liangyou Hua of rice variety C under the spraying of the rice serum with different concentrations of the naphthylacetic acid. The result shows that the independent spraying of the naphthylacetic acid or the oryza sativa serum can improve the yield of the paddy rice, and the effect varies according to the spraying concentration. Wherein, when the spray concentration of the naphthylacetic acid is 20 mu mol/L, the yield increase amplitude of the rice is 4.31 percent; the sprayed rice white (the volume percentage concentration is 0.22%) has a certain increase effect on the rice yield, and the yield increase is about 4.08%. In addition, the effect of spraying leucine alone on the rice yield is small.
TABLE 1 influence of spray application of naphthylacetic acid on rice yield (2021)
The same letter in the same column indicates that the difference is not significant at the 5% level.
According to the single-component concentration spraying test result, 20 mu mo L/L of naphthylacetic acid, 0.22% of oryza sativa serum (v/v) and 0.2% of leucine (m/m) are selected for a compounding test. Table 2 shows the variation of rice yield when different formulations were sprayed. The result shows that the spray application of the naphthylacetic acid and the oryza sativa can improve the yield of the paddy rice, and the average yield increase amplitude of Chun you 927 and Yongyou 7860 is 5.88 percent and 5.22 percent respectively; the compound of the spray naphthylacetic acid, the oryza sativa serum and the leucine obviously increases the yield of the paddy rice by about 7.66-11.17 percent.
TABLE 2 influence of spray application of Naphthol and oryza sativa composition on rice yield (2021)
Different letters within the same line of the same cultivar indicate a significant difference between treatments at the 5% level.
From the viewpoint of yield composition, the complex application of naphthylacetic acid, oryza sativa L.and leucine has no significant effect on the number of rice ears and grains per ear, but the fruiting rate and grain weight of the rice ears are significantly increased, and the yield is further significantly increased by 10.29% -11.34% (Table 3). The effect of the treatment with the agent on the pollen grains and germination rate of the rice glume heads was measured, and the result shows that the pollen grains of the rice glume heads are remarkably increased and the germination rate of the pollen grains is remarkably improved after the compound of the naphthylacetic acid, the oryza sativa serum and the leucine is sprayed (table 4). The method shows that the grain number of stigma pollen and the germination rate of pollen are increased after the compound pesticide combination is sprayed, the success rate of fertilization and setting of rice is effectively promoted, the setting rate is further improved, and the final yield is obviously increased.
TABLE 3 influence of the combination of Naphthol, oryza sativa serum and leucine on the yield of rice (2022)
The different letters of the same variety in the same column indicate significant differences between treatments at the 0.05 level, as shown below.
TABLE 4 influence of spray application of Naphtacetic acid, oryza sativa serum and leucine on the number of grains and germination rate of glume-like heads of oryza sativa (2022)
Measurement of each physiological index of rice leaves shows that the compounding of the spray application of the naphthylacetic acid, the oryza sativa serum and the leucine remarkably increases the chlorophyll content and the photosynthetic rate of the leaves in the later stage of grain grouting, and reduces the content of malondialdehyde and hydrogen peroxide (Table 5). An increase in leaf chlorophyll content during the grouted period indicates a decrease in leaf senescence, thus facilitating maintenance of a higher net photosynthetic rate, production of more photosynthetic assimilates, and promotion of grouted carbohydrate accumulation. The content of malondialdehyde and hydrogen peroxide which are products of membrane lipid peroxidation injury are reduced, which shows that the compound of the spray application of the naphthylacetic acid, the oryza sativa serum and the leucine can effectively relieve the physiological activity decline of leaf organs of plants in the later period of grouting, improve the physiological metabolism such as photosynthesis and increase the accumulation of assimilates; the source is sufficient, the grain grouting is smooth, thus the setting rate and grain weight are increased, and the yield is improved.
TABLE 5 influence of spray application of Naphthol, oryza sativa serum and leucine on physiological indicators of rice leaves (2022)
Further explores the spraying concentration of the combination of the naphthylacetic acid, the oryza sativa L.and the leucine. The results are shown in Table 6 below.
TABLE 6 influence of spraying naphthalene acetic acid and oryza sativa serum at different concentrations on rice yield (2022)
As can be seen from Table 6, the effect of different concentrations of the spray naphthalene acetic acid, the oryza sativa serum and the leucine on the yield of the rice is different. The yield of 10-50 mu mol/L naphthylacetic acid, 0.1-0.5% oryza sativa L.and 0.1-0.5% leucine can be obviously increased, the yield increase range reaches 8.9-12.6%, and the yield increase effect is the maximum by compounding and combining 10 mu mol/L naphthylacetic acid, 0.1% oryza sativa L.and 0.1% leucine, and the amplification is 11.4-12.6%.
The above description is only of the preferred embodiments of the present invention, and is not intended to limit the invention in any way, and some simple modifications, equivalent variations or modifications can be made by those skilled in the art using the teachings disclosed herein, which fall within the scope of the present invention.

Claims (8)

1. The plant growth regulator for promoting rice setting and grouting features that the effective components include naphthalene acetic acid, bactericide and leucine in the concentration of 10-50 mu mol/L, bactericide in 0.1-0.5 wt% and leucine in 0.1-0.5 wt%.
2. The plant growth regulator for promoting rice setting and grouting as claimed in claim 1, wherein the bactericide is oryza sativa serum.
3. The plant growth regulator for promoting rice setting and grouting as claimed in claim 2, wherein the content of the effective ingredient in the rice whey is 9% pyraclostrobin.
4. A plant growth regulator for promoting rice setting grouting as defined in any one of claims 1 to 3, wherein the naphthalene acetic acid is 10 to 20 μmol/L, the volume percentage concentration of the bactericide is 0.1 to 0.22%, and the mass percentage concentration of the leucine is 0.1 to 0.2%.
5. The plant growth regulator for promoting rice setting grouting according to claim 4, wherein naphthalene acetic acid is 10 μmol/L, the volume percentage concentration of the bactericide is 0.1%, the mass percentage concentration of leucine is 0.1%;
or, the naphthalene acetic acid is 20 mu mol/L, the volume percentage concentration of the bactericide is 0.2-0.22%, and the mass percentage concentration of the leucine is 0.2%.
6. The plant growth regulator for promoting the setting and grouting of paddy rice according to any one of claims 1 to 5, wherein the plant growth regulator further comprises an auxiliary agent to be formulated into a formulation suitable for agricultural use.
7. A method of using the plant growth regulator for promoting rice setting and grouting as defined in any one of claims 1 to 6, wherein the mixed aqueous solution of the plant growth regulator is applied to the leaf surface of rice and the ear of rice for spraying at the flowering stage and the middle stage of grain filling of rice respectively.
8. Use of a plant growth regulator for promoting rice grain setting and filling according to any one of claims 1 to 6, for promoting rice grain setting and filling, increasing grain setting rate and grain weight, and increasing rice yield.
CN202410208760.5A 2024-02-26 2024-02-26 Plant growth regulator for promoting rice fruiting and grouting and use method and application thereof Pending CN118084565A (en)

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