CN109452311B - Plant growth regulating composition, preparation and application thereof - Google Patents
Plant growth regulating composition, preparation and application thereof Download PDFInfo
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- CN109452311B CN109452311B CN201811519318.5A CN201811519318A CN109452311B CN 109452311 B CN109452311 B CN 109452311B CN 201811519318 A CN201811519318 A CN 201811519318A CN 109452311 B CN109452311 B CN 109452311B
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- active ingredient
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Abstract
The invention discloses a plant growth regulating composition, a preparation and application thereof, wherein the composition comprises: active ingredient A and active ingredient B; the active component A is strigolactones plant growth regulator, and the active component B is 6-benzylaminopurine; the weight ratio of the active ingredient A to the active ingredient B is 1: 1-100; the growth of the tobacco leaves can be adjusted, the germination and growth of axillary buds of the tobacco are reduced, and the yield and the quality of the tobacco leaves are improved.
Description
Technical Field
The invention relates to the technical field of pesticides, and particularly relates to a plant growth regulating composition, a preparation and application thereof.
Background
The tobacco is a leaf economic crop, the reproductive growth of the tobacco is prevented by topping, the enlargement of the leaf can be promoted, and the full development and maturity of the middle and upper leaves are particularly facilitated, so that the yield and the quality of the tobacco leaves are improved. But the tobacco plant axillary buds grow vigorously while topping, and 2-3 or more axillary buds can be regenerated for each 1 leaf axillary of the tobacco plant. If the tobacco leaves are allowed to grow, the tobacco leaves compete for nutrients and water with the growth of the tobacco leaves, a large amount of nutrients are consumed, the growth and the fullness of the main stem leaves are influenced, the tobacco leaves at the middle lower part of the tobacco plant are not full, the identity is light, the oil content is less, the elasticity is poor, and the taste and the aroma are reduced; the upper leaves grow small and thin, which results in reduced yield and quality, aggravates insect pest occurrence and disease infection in the current year, and enlarges the insect pest population in the next year. Therefore, the method has more important practical significance for ensuring the normal growth and development of the tobacco leaves left on the tobacco plants after topping and forming ideal yield and quality by controlling the growth of the axillary buds or removing tobacco branches in time after topping.
However, after topping, the apical dominance disappeared and the axillary buds had strong re-germination properties. The bud picking is carried out for 4-6 times by manual work, on one hand, the bud picking by manual work is difficult to be carried out completely, and a large amount of manpower is consumed; on the other hand, the axillary buds have strong regeneration, consume a large amount of nutrients, influence the growth of harvested leaves, delay the harvesting due to insufficient accumulation of assimilates, and reduce the quality of tobacco leaves. Ultimately resulting in less than ideal yields and quality.
Disclosure of Invention
In view of the above, the present application provides a plant growth regulating composition, a preparation and an application thereof, wherein the composition or the preparation containing the composition can regulate the growth of tobacco leaves, reduce the germination and growth of axillary buds of the tobacco leaves, and improve the yield and quality of the tobacco leaves.
The technical scheme provided by the invention is a plant growth regulating composition, which comprises the following components: active ingredient A and active ingredient B; the active component A is strigolactones plant growth regulator, and the active component B is 6-benzylaminopurine; the weight ratio of the active ingredient A to the active ingredient B is 1: 1-100.
Preferably, the weight ratio of the active ingredient A to the active ingredient B is 1: 10-100.
Preferably, the weight ratio of the active ingredient A to the active ingredient B is 1: 20.
preferably, the active ingredient A is strigolactone synthetic analogue GR 24.
Preferably, the composition consists of the active ingredient a and the active ingredient B.
The invention also provides a plant growth regulating composition preparation which consists of the plant growth regulating composition and pharmaceutically acceptable auxiliary materials.
Preferably, the formulation is prepared in a manner already known in the art.
Preferably, the preparation of the formulation comprises: mixing the composition with the auxiliary materials, and crushing and/or stirring for dissolving and/or grinding.
Preferably, the preparation formulation is missible oil, spray, dilutable solution, dilutable emulsion, wettable powder, soluble powder, suspending agent, granule or microcapsule.
Preferably, the auxiliary materials are selected from one or more of inorganic fillers, solvents, organic cosolvents, antifoaming agents, preservatives, thickeners and surfactants.
Preferably, the content of the composition in the preparation is 0.001-99% by mass percentage.
Preferably, the content of the composition in the preparation is 0.001-50% by mass percentage.
Preferably, the content of the composition in the preparation is 1-50% by mass percentage.
Preferably, the content of the composition in the preparation is 10-50% by mass percentage.
The invention also provides application of the plant growth regulating composition in regulating the growth of tobacco leaves.
Preferably, the composition is applied to the reduction of the germination of axillary buds of tobacco plants, the increase of the area of tobacco leaves, the improvement of the quality of the tobacco leaves and the increase of the yield of the tobacco leaves.
Preferably, the method of applying the plant growth regulating composition comprises: the application amount of the composition is 10 g/ha-1000 g/ha.
Preferably, the composition is applied in an amount of 100g/ha to 500 g/ha.
Preferably, the composition is applied in an amount of 150g/ha to 300 g/ha.
The strigolactones plant growth regulator A can regulate the growth processes of lateral bud elongation, plant height, leaf shape, senescence, seed germination, lateral root growth and the like, and the strigolactones can reduce the transportation effect of the auxin by applying the strigolactones to monocotyledons and dicotyledons so as to reduce the occurrence of branches. Furthermore, the strigolactone synthetic analogue GR24 is used as an environmental stimulus model and can induce the germination of weed seeds. As allelochemicals, the plant allelochemicals can promote the growth of mycorrhiza and coordinate the transportation and distribution of the substances with plants better. The application of strigolactone synthetic analogue GR24 with proper concentration on plants can reduce the level of auxin and cytokinin hormones at the corresponding parts.
The active ingredient B6-benzylaminopurine broad-spectrum plant growth regulator can promote plant cell growth, delay leaf senescence, inhibit the decomposition of chlorophyll, nucleic acid and protein in plant leaves, and protect green and prevent aging; the regulation of amino acids, auxin, inorganic salts, etc. to the treated part can promote the division and growth of cell. Treatment of tobacco plants with 6-benzylaminopurine alone promotes the growth of tobacco leaves, but also promotes the germination and growth of axillary buds.
In the present invention, "tobacco" is synonymous with "tobacco plant"; "axillary region" and "junction of tobacco petiole and stem"; the term "tobacco axillary bud" is synonymous with "tobacco leaf axillary bud", and refers to a bud formed at the junction of the tobacco leaf stalk and stem. The axillary buds grow when the hormone level homophilicity is accumulated to a certain extent at the axillary part of the leaf. When the nutrient is sufficient, the tobacco leaves can be developed into branches, and in order to improve the yield and the quality of the tobacco, the tobacco leaves are generally removed by adopting an artificial chemical method. In the present invention, the effect of the composition can be reflected by evaluating the number of axillary buds.
Compared with the prior art, the detailed description of the application is as follows:
the application of the active ingredient A obviously reduces the level of cytokinin at the growth points of the leaf axils, can obviously reduce the germination of cryptocaryon, basically has no effect of inhibiting the growth of leaves with the length of more than 10cm, does not influence the continuous growth of the leaves, but has obvious effect of inhibiting the growth of tobacco leaves with the length of less than 10cm, and leads to the increase of the ratio of lobules. The active ingredient B is singly used, a large amount of photosynthetic assimilates are transported to the leaves, the accepting capability of photosynthetic products of the leaves is obviously enhanced, the area of the leaves is increased, the quality is improved, but the content of cytokinin at axillary parts of the leaves is improved, the germination of hidden buds at the axillary parts of the leaves can be promoted, the proportion of small leaves is increased, and the quality sum of tobacco leaves is reduced. When the active ingredient A is used alone, the increase in leaf area of less than 10cm is also suppressed in suppressing the growth of axillary buds, and when the active ingredient B is used alone, the increase in leaf area is promoted, the risk of axillary bud growth is also increased.
The plant growth regulating composition provided by the invention is prepared by screening and proportioning the components and compounding the active ingredient A and the active ingredient B, so that the defect that the number of small leaves is increased when the small leaves are used singly is overcome, and the yield and the grade of tobacco leaves are improved. The composition or preparation containing the composition is used as tobacco leaf, and active ingredient B can promote new leaf with length less than 10cm to grow rapidly, and active ingredient A can make cytokinin at growth point of leaf axillary be still at low level, and reduce germination of hidden bud at leaf axillary. Therefore, the plant growth regulating composition or the preparation containing the composition is used for the tobacco leaves, the proportion of large leaves in the harvested tobacco leaves is increased, the consumption of assimilates on the growth of axillary buds and low-value small leaves is avoided, and the yield and the quality of the tobacco leaves are improved. The plant growth regulating composition or the preparation containing the composition provided by the invention is used for tobacco plants, can reduce the germination and growth of hidden buds at axillary leaves, has obvious effect of increasing yield and has synergistic effect.
Detailed Description
In order to make those skilled in the art better understand the technical solution of the present invention, the following detailed description of the present invention is provided with reference to specific embodiments.
Example 1
Wettable powder
The preparation method of the strigolactone artificially synthesized analogue GR 24.6-benzylaminopurine wettable powder comprises the following steps: dissolving strigolactone artificially synthesized analogue GR24 in acetone with the same weight, adsorbing with white carbon black, removing acetone at 40 ℃, uniformly mixing with 6-benzylaminopurine, polycarboxylate, fatty alcohol polyoxyethylene ether and diatomite, and performing jet milling to obtain the strigolactone analogue.
Example 2
Water dispersible granule
The preparation method of the strigolactone artificially synthesized analogue GR 24.6-benzylaminopurine water dispersible granule comprises the following steps: dissolving strigolactone artificially synthesized analogue GR24 with the same weight of acetone, adsorbing with white carbon black, removing the acetone at the temperature of 40 ℃, uniformly mixing with 6-benzylaminopurine, polycarboxylate, sodium lignosulfonate, corn starch and diatomite, carrying out air flow crushing, adding 2-5% of atomized water for wetting, kneading, granulating and drying to obtain the strigolactone.
Example 3
Dispersible oil suspension
The preparation method of the strigolactone artificially synthesized analogue GR 24.6-benzylaminopurine dispersible oil suspending agent comprises the following steps: artificially synthesized strigolactone analogues GR24, 6-benzylaminopurine, BREAK-THRUDA646, BREAK-THRUSD260, fumed silica A-974 and methyl oleate are ground until D98 is less than 3um, and discharged for packaging.
Example 4
1. Test subjects: tobacco leaves.
2. The experimental method comprises the following steps: referring to 'indoor bioassay test criteria for pesticides NY/T2061.2-2011' part 2 of plant growth regulators: stem and leaf spraying for plant growth promotion and inhibition test, and pot culture method; the method for treating the tobacco leaves by using the composition and the single agent after the components are mixed comprises the following steps: spraying, after the pesticide is applied, naturally drying the surface liquid medicine, moving the liquid medicine to an indoor room for growth, and observing the growth condition; the application time is as follows: and (4) topping the tobacco plants.
3. Data statistical analysis: the analysis was performed using DPS7.05(Duncan) statistical software. Calculating the average yield per mu C according to the test data1(kg), average yield per mu increase rate D over blank control1(％)。
4. The evaluation method comprises the following steps:
evaluation index D of actually measured mixture of components1Theoretical index E obtained by the Gowing formula1Comparing;
differences between the actual and theoretical values of greater than 10% are synergistic effects, differences between the actual and theoretical values of ± 10% are additive effects, and differences between the actual and theoretical values of less than-10% are antagonistic effects.
The calculation formula is as follows:
increase rate ═ (test data-blank)/blank 100%;
gowing formula: e ═ x + y-xy/100; x and y are actual increasing rate or actual decreasing rate obtained by using each component alone respectively.
5. The test results show that the composition using the mixed components has no phytotoxicity such as leaf deformity and green loss, and the results are shown in Table 1.
TABLE 1
As can be seen from the above table, the weight ratio of the active ingredient a to the active ingredient B is 1: 1-100 hours, the plant growth regulating composition provided by the invention has an obvious effect of improving the yield of tobacco leaves and has a synergistic effect.
Example 5
1. Test subjects: tobacco leaves.
2. The experimental method comprises the following steps: refer to GB-T17980.139-2004-pesticide field efficacy test criteria; respectively treating tobacco leaves by using a composition and a single agent after the components are mixed, wherein the application method is spraying, naturally drying the surface liquid medicine after the application, moving the liquid medicine to an indoor room for growth, and observing the growth condition; the application time is as follows: and (4) topping the tobacco plants.
3. Data statistical analysis: the analysis was performed using DPS7.05(Duncan) statistical software. Selecting 20 points for each treatment, measuring the axillary buds of 20 tobacco leaves for each point, and calculating the average axillary bud number C of 20 tobacco leaves according to test data2(one), calculating the average axillary bud number reduction rate D relative to the blank control2(％)。
4. The evaluation method comprises the following steps:
evaluation index D of actually measured mixture of components2Theoretical index E obtained by the Gowing formula2Comparing; the difference between the actual value and the theoretical value is more than 10 percent, the actual value and the theoretical value are the synergistic effectDifferences between the theoretical values of + -10% are additive effects, differences between the actual values and the theoretical values of less than-10% are antagonistic effects.
The calculation formula is as follows:
increase rate ═ (test data-blank)/blank 100%;
gowing formula: e ═ x + y-xy/100; x and y are actual increasing rate or actual decreasing rate obtained by using each component alone respectively.
5. The test results show that the composition using the mixed components has no phytotoxicity such as leaf deformity and green loss, and the results are shown in tables 2 and 3.
TABLE 2
As can be seen from the above table, the single-dose application of the active ingredient B has the effect of promoting the germination of the cryptophyte. The weight ratio of the active ingredient A to the active ingredient B is 1: 1-100 hours, the plant growth regulating composition provided by the invention can reduce the germination of hidden buds of tobacco leaves to grow into axillary buds, has obvious effect and has a synergistic effect.
The above is only a preferred embodiment of the present invention, and it should be noted that the above preferred embodiment should not be considered as limiting the present invention, and the protection scope of the present invention should be subject to the scope defined by the claims. It will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the spirit and scope of the invention, and these modifications and adaptations should be considered within the scope of the invention.
Claims (9)
1. A plant growth regulating composition, comprising: active ingredient A and active ingredient B; the active ingredient A is strigolactone artificially synthesized analogue GR24, and the active ingredient B is 6-benzylaminopurine; the weight ratio of the active ingredient A to the active ingredient B is 1: 1-100.
2. The composition according to claim 1, characterized in that the weight ratio of active ingredient a to active ingredient B is 1: 10-100.
3. The composition according to claim 1, characterized in that the weight ratio of active ingredient a to active ingredient B is 1: 20.
4. the composition according to claim 1, characterized in that it consists of said active ingredient a and said active ingredient B.
5. A plant growth regulating composition formulation, comprising the plant growth regulating composition of any one of claims 1-4 and a pharmaceutically acceptable adjuvant.
6. The formulation of claim 5, wherein the formulation is an emulsifiable concentrate, a spray, a dilutable solution, a dilutable emulsion, a wettable powder, a soluble powder, a dust, a suspension, a granule, or a microcapsule.
7. Use of a plant growth regulating composition according to any one of claims 1 to 4 for regulating the growth of tobacco leaves.
8. Use according to claim 7, wherein the composition is used for reducing the germination and growth of axillary buds of tobacco, improving the quality of tobacco leaves, and increasing the yield of tobacco leaves.
9. Use according to claim 7, wherein the plant growth regulating composition is applied by a method comprising: the application amount of the composition is 10 g/ha-1000 g/ha.
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| CN114158570B (en) * | 2021-12-13 | 2022-10-21 | 四川中烟工业有限责任公司 | Compound agent for reducing stem content of tobacco leaves as well as preparation method and application thereof |
| CN115363045A (en) * | 2022-04-15 | 2022-11-22 | 福建省烟草公司三明市公司 | Protective agent for preventing plant leaves from sunburn and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2999385A1 (en) * | 2012-12-14 | 2014-06-20 | Nufarm | Liquid solution, useful for treating plants and vegetables, comprises plant growth regulator e.g. gibberellin A3 and polar aprotic solvent including 1,3-dioxolan-4-yl-methanol compound, 1,3-dioxan-5-ol compound and/or dicarbonyl compound |
| CN105849104A (en) * | 2013-10-25 | 2016-08-10 | 阿西洛玛生物公司 | Strigolactone formulations and uses thereof |
| CN108849897A (en) * | 2018-08-15 | 2018-11-23 | 中国农业科学院油料作物研究所 | A kind of drought-enduring coating agent for seed of rape |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2999385A1 (en) * | 2012-12-14 | 2014-06-20 | Nufarm | Liquid solution, useful for treating plants and vegetables, comprises plant growth regulator e.g. gibberellin A3 and polar aprotic solvent including 1,3-dioxolan-4-yl-methanol compound, 1,3-dioxan-5-ol compound and/or dicarbonyl compound |
| CN105849104A (en) * | 2013-10-25 | 2016-08-10 | 阿西洛玛生物公司 | Strigolactone formulations and uses thereof |
| CN108849897A (en) * | 2018-08-15 | 2018-11-23 | 中国农业科学院油料作物研究所 | A kind of drought-enduring coating agent for seed of rape |
Non-Patent Citations (1)
| Title |
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| 新型植物激素独脚金内酯的研究进展;陈虞超等;《中国农学通报》;20151231;第31卷(第24期);第157-162页 * |
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