CN118019845A - tRNA、氨酰基tRNA、多肽合成用试剂、非天然氨基酸的导入方法、多肽的制作方法、核酸展示文库的制作方法、核酸-多肽连接体及筛选方法 - Google Patents

tRNA、氨酰基tRNA、多肽合成用试剂、非天然氨基酸的导入方法、多肽的制作方法、核酸展示文库的制作方法、核酸-多肽连接体及筛选方法 Download PDF

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CN118019845A
CN118019845A CN202280063245.1A CN202280063245A CN118019845A CN 118019845 A CN118019845 A CN 118019845A CN 202280063245 A CN202280063245 A CN 202280063245A CN 118019845 A CN118019845 A CN 118019845A
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trna
polypeptide
nucleic acid
codon
amino acid
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Chinese (zh)
Inventor
石井由纪子
富永让
木原史织
芳坂贵弘
渡边贵嘉
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Fujifilm Corp
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Fujifilm Corp
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1044—Preparation or screening of libraries displayed on scaffold proteins
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1062—Isolating an individual clone by screening libraries mRNA-Display, e.g. polypeptide and encoding template are connected covalently
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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    • C12N2320/00—Applications; Uses
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    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C12N2330/31—Libraries, arrays

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • Engineering & Computer Science (AREA)
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  • Biomedical Technology (AREA)
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  • Wood Science & Technology (AREA)
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  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202280063245.1A 2021-09-24 2022-09-26 tRNA、氨酰基tRNA、多肽合成用试剂、非天然氨基酸的导入方法、多肽的制作方法、核酸展示文库的制作方法、核酸-多肽连接体及筛选方法 Pending CN118019845A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021-156183 2021-09-24
JP2021156183 2021-09-24
PCT/JP2022/035791 WO2023048291A1 (ja) 2021-09-24 2022-09-26 ｔＲＮＡ、アミノアシルｔＲＮＡ、ポリペプチド合成用試薬、非天然アミノ酸の導入方法、ポリペプチドの作製方法、核酸ディスプレイライブラリの作製方法、核酸－ポリペプチド連結体及びスクリーニング方法

Publications (1)

Publication Number Publication Date
CN118019845A true CN118019845A (zh) 2024-05-10

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CN202280063245.1A Pending CN118019845A (zh) 2021-09-24 2022-09-26 tRNA、氨酰基tRNA、多肽合成用试剂、非天然氨基酸的导入方法、多肽的制作方法、核酸展示文库的制作方法、核酸-多肽连接体及筛选方法

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US (1) US20240229015A1 (https=)
EP (1) EP4389890A4 (https=)
JP (1) JPWO2023048291A1 (https=)
CN (1) CN118019845A (https=)
WO (1) WO2023048291A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024214800A1 (ja) * 2023-04-11 2024-10-17 富士フイルム株式会社 生理活性評価用のポリペプチド溶液を調製する方法、及びポリペプチドの生理活性を評価する方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003281613A1 (en) 2002-07-18 2004-02-09 Takahiro Hohsaka NON-NATURAL LABELED AMINO ACID AND METHOD OF CONSTRUCTING AMINO ACID/tRNA COMPLEX
WO2007055429A1 (ja) 2005-11-14 2007-05-18 Japan Science And Technology Agency 非天然アミノ酸をタンパク質に導入するためのtRNA変異体
JP2008193911A (ja) * 2007-02-08 2008-08-28 Protein Express:Kk タンパク質のｎ末端を特異的に修飾する方法
JP5818237B2 (ja) * 2010-09-09 2015-11-18 国立大学法人 東京大学 Ｎ−メチルアミノ酸およびその他の特殊アミノ酸を含む特殊ペプチド化合物ライブラリーの翻訳構築と活性種探索法
RU2731211C1 (ru) 2017-03-21 2020-08-31 Фуджифилм Корпорэйшн Пептидное соединение и способ его получения, композиция для скринингового использования и способ отбора пептидного соединения
KR102930465B1 (ko) * 2017-11-02 2026-02-25 더 위스타 인스티튜트 오브 아나토미 앤드 바이올로지 ACE-tRNA를 이용한 유전 재할당을 통한 정지 코돈의 구조 방법
US20220325269A1 (en) * 2019-08-08 2022-10-13 Brickbio, Inc. Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids
JP7211389B2 (ja) 2020-03-25 2023-01-24 トヨタ自動車株式会社 内燃機関の制御装置

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EP4389890A1 (en) 2024-06-26
WO2023048291A1 (ja) 2023-03-30
US20240229015A1 (en) 2024-07-11
EP4389890A4 (en) 2025-12-31
JPWO2023048291A1 (https=) 2023-03-30

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