CN118000316A - Feed additive containing plant polysaccharide and preparation method and application thereof - Google Patents
Feed additive containing plant polysaccharide and preparation method and application thereof Download PDFInfo
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 86
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 86
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 86
- 239000003674 animal food additive Substances 0.000 title claims abstract description 56
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 241000196324 Embryophyta Species 0.000 claims abstract description 77
- 239000000843 powder Substances 0.000 claims abstract description 51
- 239000002994 raw material Substances 0.000 claims abstract description 20
- 150000001875 compounds Chemical class 0.000 claims abstract description 17
- 241000512259 Ascophyllum nodosum Species 0.000 claims abstract description 15
- 240000002900 Arthrospira platensis Species 0.000 claims abstract description 13
- 235000016425 Arthrospira platensis Nutrition 0.000 claims abstract description 13
- 244000144977 poultry Species 0.000 claims abstract description 13
- 229940082787 spirulina Drugs 0.000 claims abstract description 13
- 244000068988 Glycine max Species 0.000 claims abstract description 12
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 12
- GOMNOOKGLZYEJT-UHFFFAOYSA-N isoflavone Chemical compound C=1OC2=CC=CC=C2C(=O)C=1C1=CC=CC=C1 GOMNOOKGLZYEJT-UHFFFAOYSA-N 0.000 claims abstract description 12
- CJWQYWQDLBZGPD-UHFFFAOYSA-N isoflavone Natural products C1=C(OC)C(OC)=CC(OC)=C1C1=COC2=C(C=CC(C)(C)O3)C3=C(OC)C=C2C1=O CJWQYWQDLBZGPD-UHFFFAOYSA-N 0.000 claims abstract description 12
- 235000008696 isoflavones Nutrition 0.000 claims abstract description 12
- 241000251468 Actinopterygii Species 0.000 claims abstract description 9
- 244000144972 livestock Species 0.000 claims abstract description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 72
- 239000011259 mixed solution Substances 0.000 claims description 52
- 238000010438 heat treatment Methods 0.000 claims description 25
- 238000002791 soaking Methods 0.000 claims description 23
- 240000001008 Dimocarpus longan Species 0.000 claims description 20
- 235000000235 Euphoria longan Nutrition 0.000 claims description 20
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 18
- 238000001035 drying Methods 0.000 claims description 18
- 235000019441 ethanol Nutrition 0.000 claims description 18
- 239000007788 liquid Substances 0.000 claims description 18
- 239000000243 solution Substances 0.000 claims description 18
- 239000002904 solvent Substances 0.000 claims description 18
- 239000012153 distilled water Substances 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 11
- 238000001704 evaporation Methods 0.000 claims description 7
- 238000009210 therapy by ultrasound Methods 0.000 claims description 7
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 claims description 6
- 235000019743 Choline chloride Nutrition 0.000 claims description 6
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 claims description 6
- 229960003178 choline chloride Drugs 0.000 claims description 6
- 238000000227 grinding Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 238000000926 separation method Methods 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 239000002131 composite material Substances 0.000 claims 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 abstract description 18
- 241001465754 Metazoa Species 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 8
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- 238000003786 synthesis reaction Methods 0.000 abstract description 6
- AUYYCJSJGJYCDS-LBPRGKRZSA-N Thyrolar Chemical class IC1=CC(C[C@H](N)C(O)=O)=CC(I)=C1OC1=CC=C(O)C(I)=C1 AUYYCJSJGJYCDS-LBPRGKRZSA-N 0.000 abstract description 3
- 210000001789 adipocyte Anatomy 0.000 abstract description 3
- 230000001939 inductive effect Effects 0.000 abstract description 3
- 235000019626 lipase activity Nutrition 0.000 abstract description 3
- 230000004130 lipolysis Effects 0.000 abstract description 3
- 239000006041 probiotic Substances 0.000 abstract description 3
- 235000018291 probiotics Nutrition 0.000 abstract description 3
- 239000005495 thyroid hormone Substances 0.000 abstract description 3
- 229940036555 thyroid hormone Drugs 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 230000003064 anti-oxidating effect Effects 0.000 abstract description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract description 2
- 230000006372 lipid accumulation Effects 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 11
- 235000013594 poultry meat Nutrition 0.000 description 11
- 241000287828 Gallus gallus Species 0.000 description 6
- 235000013330 chicken meat Nutrition 0.000 description 6
- 238000012360 testing method Methods 0.000 description 5
- 239000004519 grease Substances 0.000 description 3
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 230000035508 accumulation Effects 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 238000013329 compounding Methods 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 210000002865 immune cell Anatomy 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 235000003687 soy isoflavones Nutrition 0.000 description 2
- 241001474374 Blennius Species 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- 208000007976 Ketosis Diseases 0.000 description 1
- 206010057249 Phagocytosis Diseases 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 150000001323 aldoses Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000005859 cell recognition Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000004154 complement system Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 125000005456 glyceride group Chemical group 0.000 description 1
- 150000002327 glycerophospholipids Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 230000031891 intestinal absorption Effects 0.000 description 1
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 1
- 150000002584 ketoses Chemical class 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000008782 phagocytosis Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000003270 steroid hormone Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Birds (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Insects & Arthropods (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Materials Engineering (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Medicinal Chemistry (AREA)
- Marine Sciences & Fisheries (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Physiology (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention relates to a feed additive containing plant polysaccharide, and a preparation method and application thereof, belonging to the technical field of feed additives. The invention discloses a feed additive containing plant polysaccharide, which comprises the following raw materials in parts by weight: 50-70 parts of compound plant polysaccharide, 10-15 parts of kelp powder, 5-10 parts of soybean isoflavone and 10-15 parts of spirulina powder. The polysaccharide can promote the activity of probiotics, activate the antioxidation capability, and in addition, the polysaccharide helps to release free glycerol by inducing lipolysis of fat cells, so that lipid accumulation is reduced, and meanwhile, the lipase activity is inhibited to obviously reduce the glycerol absorption in intestinal tracts; kelp powder and soybean isoflavone promote the synthesis of thyroid hormone and improve animal activity. The feed additive containing plant polysaccharide prepared by the invention can be applied to fish feed, poultry feed and livestock feed.
Description
Technical Field
The invention belongs to the technical field of feed additives, and relates to a feed additive containing plant polysaccharide, and a preparation method and application thereof.
Background
The cultivation mode is divided into traditional cultivation and intensive cultivation, and the intensive cultivation is a main form of cultivation scale due to site limitation and more convenient management. The intensive cultivation can improve the production efficiency of poultry. Through scientific feeding management and environmental control, the growth and the weight growth of the poultry can be optimized, the feed conversion rate is improved, and the waste of resources is reduced, so that the economic benefit of cultivation is improved. However, the intensive animal cultivation is limited in activity, a large amount of nutrient components are contained in the feed, and the excessive nutrient components are converted into fat which is accumulated in the animal body, so that the health is influenced, and the taste is seriously influenced.
Polysaccharides are a class of natural macromolecular polymers linked by glycosidic linkages between aldoses or ketoses. Polysaccharide is widely available in nature, and especially in higher plants, animals, microorganisms and seaweed. Polysaccharides play an extremely important role in cell recognition, control of cell division and differentiation, and the like. The active polysaccharide can directly activate immune cells, enhance phagocytosis and killing effect of animal immune cells, activate complement system, promote antibody production, and synthesize and secrete cytokines.
Disclosure of Invention
The invention relates to a feed additive containing plant polysaccharide, and a preparation method and application thereof, belonging to the technical field of feed additives. The invention discloses a feed additive containing plant polysaccharide, which comprises the following raw materials in parts by weight: 50-70 parts of compound plant polysaccharide, 10-15 parts of kelp powder, 5-10 parts of soybean isoflavone and 10-15 parts of spirulina powder. The polysaccharide can promote the activity of probiotics, activate the antioxidation capability, and in addition, the polysaccharide helps to release free glycerol by inducing lipolysis of fat cells, so that lipid accumulation is reduced, and meanwhile, the lipase activity is inhibited to obviously reduce the glycerol absorption in intestinal tracts; kelp powder and soybean isoflavone promote the synthesis of thyroid hormone and improve animal activity. The feed additive containing plant polysaccharide prepared by the invention can be applied to fish feed, poultry feed and livestock feed.
The aim of the invention can be achieved by the following technical scheme:
the feed additive containing the plant polysaccharide comprises the following raw materials in parts by weight: 50-70 parts of compound plant polysaccharide, 10-15 parts of kelp powder, 5-10 parts of soybean isoflavone and 10-15 parts of spirulina powder.
Further, the preparation method of the compound plant polysaccharide comprises the following steps:
a: removing shells and cores of longan, sun drying, grinding into longan powder, soaking in ethanol solution, and centrifuging to obtain mixed solution a;
b: adding distilled water into the mixed solution a, heating and stirring, standing and filtering to obtain a mixed solution b;
C: removing the core of the red date, adding absolute ethyl alcohol for soaking, and then drying to obtain a red date stock;
d: crushing the red date stock into red date powder, adding a solvent, mixing to form a mixture, carrying out ultrasonic treatment under pressure, and filtering to obtain a mixed solution c;
e: mixing the mixed solution b and the mixed solution c, and then heating, evaporating and concentrating to obtain the compound plant polysaccharide.
Further, in the step A, the mass fraction of the ethanol solution is 80%, the solid-to-liquid ratio of the longan powder to the ethanol solution is 1:5g/mL, the soaking time is 16-24h, and the rotating speed and the time of centrifugal separation are 4000r/min and 5-10min respectively.
Further, in the step B, the volume ratio of the mixed solution a to the distilled water is 1:15-20, the heating means heating to 80 ℃, and the standing time is 2-3h.
Further, in the step C, the solid-to-liquid ratio of the red dates to the absolute ethyl alcohol is 1:10g/mL, the soaking time of the absolute ethyl alcohol is 30-60min, and the drying temperature is 40-50 ℃.
Further, in the step D, the solid-to-liquid ratio of the red date powder to the solvent is 1:5g/mL, the solvent consists of choline chloride and ethylene glycol with the mass ratio of 1:1, the pressurizing pressure is 20-30Mpa, and the ultrasonic time is 20-30min.
Further, in the step E, the volume ratio of the mixed solution b to the mixed solution c is 1:1, and the temperature is raised to 50-60 ℃.
Further, the preparation method of the feed additive containing plant polysaccharide comprises the following steps: all the raw material components are mixed to obtain the feed additive containing plant polysaccharide.
The application of the plant polysaccharide-containing feed additive is that the plant polysaccharide-containing feed additive is applied to fish feed, poultry feed and livestock feed.
The invention has the beneficial effects that:
1. The plant polysaccharide prepared by compounding the longan and the red dates enhances the activity of probiotics, and in addition, the free glycerol is released by inducing lipolysis of fat cells, so that the accumulation of lipid is reduced, and meanwhile, the lipase activity is inhibited, so that the intestinal absorption of the glycerol is obviously reduced. The feed additive containing plant polysaccharide prepared by the invention can effectively reduce the grease content in animals.
2. The soybean isoflavone and the polysaccharide are added for compound use, the expression of related proteins and genes can be influenced through various signal paths, and the synthesis of fatty acid organisms, the synthesis of steroid hormone organisms, the synthesis of unsaturated fatty acid organisms, the elongation of fatty acid, the metabolism of glyceride and the metabolism of glycerophospholipids are influenced, so that the possibility that nutrition is converted into fat is further prevented.
3. Kelp powder and spirulina are rich in iodine, vitamins, amino acids and other substances, promote the synthesis of animal thyroid hormone, make animals more active, reduce fat accumulation through exercise and have better taste to meat quality.
Detailed Description
In order to further describe the technical means and effects adopted by the present invention for achieving the intended purpose, the following detailed description will refer to the specific embodiments, structures, features and effects according to the present invention in conjunction with examples.
Example 1
The feed additive containing the plant polysaccharide comprises the following raw materials in parts by weight: 50 parts of compound plant polysaccharide, 10 parts of kelp powder, 5 parts of soybean isoflavone and 10 parts of spirulina powder.
The preparation method of the compound plant polysaccharide comprises the following steps:
a: removing shells and cores of longan, sun drying, grinding into longan powder, soaking in ethanol solution, and centrifuging to obtain mixed solution a;
b: adding distilled water into the mixed solution a, heating and stirring, standing and filtering to obtain a mixed solution b;
C: removing the core of the red date, adding absolute ethyl alcohol for soaking, and then drying to obtain a red date stock;
d: crushing the red date stock into red date powder, adding a solvent, mixing to form a mixture, carrying out ultrasonic treatment under pressure, and filtering to obtain a mixed solution c;
e: mixing the mixed solution b and the mixed solution c, and then heating, evaporating and concentrating to obtain the compound plant polysaccharide.
In the step A, the mass fraction of the ethanol solution is 80%, the solid-to-liquid ratio of the longan powder to the ethanol solution is 1:5g/mL, the soaking time is 16h, and the rotating speed and the time of centrifugal separation are 4000r/min and 5min respectively.
In the step B, the volume ratio of the mixed solution a to the distilled water is 1:15, the heating means heating to 80 ℃, and the standing time is 2 hours.
In the step C, the solid-to-liquid ratio of the red dates to the absolute ethyl alcohol is 1:10g/mL, the soaking time of the absolute ethyl alcohol is 30min, and the drying temperature is 40 ℃.
In the step D, the solid-to-liquid ratio of the red date powder to the solvent is 1:5g/mL, the solvent consists of choline chloride and ethylene glycol with the mass ratio of 1:1, the pressurizing pressure is 20Mpa, and the ultrasonic time is 20min.
In the step E, the volume ratio of the mixed solution b to the mixed solution c is 1:1, and the temperature is raised to 50 ℃.
The preparation method of the feed additive containing plant polysaccharide comprises the following steps: all the raw material components are mixed to obtain the feed additive containing plant polysaccharide.
The application of the plant polysaccharide-containing feed additive is that the plant polysaccharide-containing feed additive is applied to fish feed, poultry feed and livestock feed.
Example 2
The feed additive containing the plant polysaccharide comprises the following raw materials in parts by weight: 60 parts of compound plant polysaccharide, 10 parts of kelp powder, 5 parts of soybean isoflavone and 10 parts of spirulina powder.
The preparation method of the compound plant polysaccharide comprises the following steps:
a: removing shells and cores of longan, sun drying, grinding into longan powder, soaking in ethanol solution, and centrifuging to obtain mixed solution a;
b: adding distilled water into the mixed solution a, heating and stirring, standing and filtering to obtain a mixed solution b;
C: removing the core of the red date, adding absolute ethyl alcohol for soaking, and then drying to obtain a red date stock;
d: crushing the red date stock into red date powder, adding a solvent, mixing to form a mixture, carrying out ultrasonic treatment under pressure, and filtering to obtain a mixed solution c;
e: mixing the mixed solution b and the mixed solution c, and then heating, evaporating and concentrating to obtain the compound plant polysaccharide.
In the step A, the mass fraction of the ethanol solution is 80%, the solid-to-liquid ratio of the longan powder to the ethanol solution is 1:5g/mL, the soaking time is 20h, and the rotating speed and the time of centrifugal separation are 4000r/min and 10min respectively.
In the step B, the volume ratio of the mixed solution a to the distilled water is 1:18, the heating means heating to 80 ℃, and the standing time is 2 hours.
In the step C, the solid-to-liquid ratio of the red dates to the absolute ethyl alcohol is 1:10g/mL, the soaking time of the absolute ethyl alcohol is 45min, and the drying temperature is 40 ℃.
In the step D, the solid-to-liquid ratio of the red date powder to the solvent is 1:5g/mL, the solvent consists of choline chloride and ethylene glycol with the mass ratio of 1:1, the pressurizing pressure is 25Mpa, and the ultrasonic time is 25min.
In the step E, the volume ratio of the mixed solution b to the mixed solution c is 1:1, and the temperature is raised to 55 ℃.
The preparation method of the feed additive containing plant polysaccharide comprises the following steps: all the raw material components are mixed to obtain the feed additive containing plant polysaccharide.
The application of the plant polysaccharide-containing feed additive is that the plant polysaccharide-containing feed additive is applied to fish feed, poultry feed and livestock feed.
Example 3
The feed additive containing the plant polysaccharide comprises the following raw materials in parts by weight: 70 parts of compound plant polysaccharide, 15 parts of kelp powder, 10 parts of soybean isoflavone and 15 parts of spirulina powder.
The preparation method of the compound plant polysaccharide comprises the following steps:
a: removing shells and cores of longan, sun drying, grinding into longan powder, soaking in ethanol solution, and centrifuging to obtain mixed solution a;
b: adding distilled water into the mixed solution a, heating and stirring, standing and filtering to obtain a mixed solution b;
C: removing the core of the red date, adding absolute ethyl alcohol for soaking, and then drying to obtain a red date stock;
d: crushing the red date stock into red date powder, adding a solvent, mixing to form a mixture, carrying out ultrasonic treatment under pressure, and filtering to obtain a mixed solution c;
e: mixing the mixed solution b and the mixed solution c, and then heating, evaporating and concentrating to obtain the compound plant polysaccharide.
In the step A, the mass fraction of the ethanol solution is 80%, the solid-to-liquid ratio of the longan powder to the ethanol solution is 1:5g/mL, the soaking time is 24 hours, and the rotating speed and the time of centrifugal separation are 4000r/min and 10min respectively.
In the step B, the volume ratio of the mixed solution a to the distilled water is 1:20, the heating means heating to 80 ℃, and the standing time is 3 hours.
In the step C, the solid-to-liquid ratio of the red dates to the absolute ethyl alcohol is 1:10g/mL, the soaking time of the absolute ethyl alcohol is 60min, and the drying temperature is 50 ℃.
In the step D, the solid-to-liquid ratio of the red date powder to the solvent is 1:5g/mL, the solvent consists of choline chloride and ethylene glycol with the mass ratio of 1:1, the pressurizing pressure is 30Mpa, and the ultrasonic time is 30min.
In the step E, the volume ratio of the mixed solution b to the mixed solution c is 1:1, and the temperature is raised to 60 ℃.
The preparation method of the feed additive containing plant polysaccharide comprises the following steps: all the raw material components are mixed to obtain the feed additive containing plant polysaccharide.
The application of the plant polysaccharide-containing feed additive is that the plant polysaccharide-containing feed additive is applied to fish feed, poultry feed and livestock feed.
Comparative example 1
On the basis of the embodiment 2, the feed additive containing the plant polysaccharide comprises the following raw materials in parts by weight: 60 parts of plant polysaccharide, 10 parts of kelp powder, 5 parts of soybean isoflavone and 10 parts of spirulina powder.
The preparation method of the plant polysaccharide comprises the following steps:
a: removing the core of the red date, adding absolute ethyl alcohol for soaking, and then drying to obtain a red date stock;
b: crushing the red date stock into red date powder, adding a solvent, mixing to form a mixture, carrying out ultrasonic treatment under pressure, and filtering to obtain a mixed solution c;
C: and heating, evaporating and concentrating the mixed solution c to obtain the plant polysaccharide.
In the step A, the solid-to-liquid ratio of the red dates to the absolute ethyl alcohol is 1:10g/mL, the soaking time of the absolute ethyl alcohol is 45min, and the drying temperature is 40 ℃.
In the step B, the solid-to-liquid ratio of the red date powder to the solvent is 1:5g/mL, the solvent consists of choline chloride and ethylene glycol with the mass ratio of 1:1, the pressurizing pressure is 25Mpa, and the ultrasonic time is 25min.
The temperature rise in the step C means that the temperature rises to 55 ℃.
The preparation method of the feed additive containing plant polysaccharide comprises the following steps: all the raw material components are mixed to obtain the feed additive containing plant polysaccharide.
The application of the plant polysaccharide-containing feed additive is that the plant polysaccharide-containing feed additive is applied to fish feed, poultry feed and livestock feed.
Comparative example 2
On the basis of the embodiment 2, the feed additive containing the plant polysaccharide comprises the following raw materials in parts by weight: 60 parts of plant polysaccharide, 10 parts of kelp powder, 5 parts of soybean isoflavone and 10 parts of spirulina powder.
The preparation method of the plant polysaccharide comprises the following steps:
a: removing shells and cores of longan, sun drying, grinding into longan powder, soaking in ethanol solution, and centrifuging to obtain mixed solution a;
b: adding distilled water into the mixed solution a, heating and stirring, standing and filtering to obtain a mixed solution b;
C: and heating, evaporating and concentrating the mixed solution b to obtain the plant polysaccharide.
In the step A, the mass fraction of the ethanol solution is 80%, the solid-to-liquid ratio of the longan powder to the ethanol solution is 1:5g/mL, the soaking time is 20h, and the rotating speed and the time of centrifugal separation are 4000r/min and 10min respectively.
In the step B, the volume ratio of the mixed solution a to the distilled water is 1:18, the heating means heating to 80 ℃, and the standing time is 2 hours.
The temperature rise in the step C means that the temperature rises to 55 ℃.
The preparation method of the feed additive containing plant polysaccharide comprises the following steps: all the raw material components are mixed to obtain the feed additive containing plant polysaccharide.
The application of the plant polysaccharide-containing feed additive is that the plant polysaccharide-containing feed additive is applied to fish feed, poultry feed and livestock feed.
Comparative example 3
On the basis of example 2, soy isoflavones were removed from the raw material components, and the other conditions were the same as in example 2.
Comparative example 4
The kelp powder was removed from the raw material component based on example 2 and replaced with spirulina powder in equal parts by weight, and the other conditions were the same as in example 2.
Comparative example 5
On the basis of example 2, the spirulina component was removed from the raw material component and replaced with the kelp powder in equal parts by weight, and the other conditions were the same as in example 2.
1. Performance testing
The feed additives containing plant polysaccharide prepared in examples 1 to 3 and comparative examples 1 to 5 were added to a poultry feed as a sample in an amount of 10%, the poultry feed consisting of the following raw materials in parts by weight: 2.0 parts of corn, 1.3 parts of sorghum, 1.7 parts of barley, she Kang 2.0.0 parts of bean cake and bean pulp, 1 part of sweet potato powder and 1.5 parts of wheat bran. Selecting 800 healthy chickens which are born for 30 days, dividing the chickens into 8 groups, feeding the chickens in a containment mode and using a sample for feeding, wherein the specific feeding mode is as follows: once in the morning, in the middle and in the evening. In addition, 100 chickens were selected as a control group using feed without additives.
Fat testing: the fat content of the chicken is measured by scanning the abdomen of the chicken with an ultrasonic instrument, and the fat increment is calculated by measuring every 1 month and testing for 3 months respectively. The results of the above tests are shown in Table 1.
Table 1 test results
As can be seen from Table 1, the grease increment per month was smaller in examples 1-3 than in comparative examples 1-5 and the control group, wherein the grease increment per month was the largest in the control group. In comparative example 1, longan is not added for compounding; comparative example 2 was not formulated with red date added; comparative example 3 lacks the addition of soy isoflavones; comparative examples 4 to 5 only one of kelp powder and spirulina powder was added. Thus the absence of either component affects the fat content.
The present invention is not limited to the above embodiments, but is capable of modification and variation in detail, and other modifications and variations can be made by those skilled in the art without departing from the scope of the present invention.
Claims (9)
1. The feed additive containing the plant polysaccharide is characterized by comprising the following raw materials in parts by weight: 50-70 parts of compound plant polysaccharide, 10-15 parts of kelp powder, 5-10 parts of soybean isoflavone and 10-15 parts of spirulina powder.
2. The plant polysaccharide-containing feed additive according to claim 1, wherein the preparation method of the composite plant polysaccharide comprises the following steps:
a: removing shells and cores of longan, sun drying, grinding into longan powder, soaking in ethanol solution, and centrifuging to obtain mixed solution a;
b: adding distilled water into the mixed solution a, heating and stirring, standing and filtering to obtain a mixed solution b;
C: removing the core of the red date, adding absolute ethyl alcohol for soaking, and then drying to obtain a red date stock;
d: crushing the red date stock into red date powder, adding a solvent, mixing to form a mixture, carrying out ultrasonic treatment under pressure, and filtering to obtain a mixed solution c;
e: mixing the mixed solution b and the mixed solution c, and then heating, evaporating and concentrating to obtain the compound plant polysaccharide.
3. The plant polysaccharide-containing feed additive according to claim 2, wherein in the step A, the mass fraction of the ethanol solution is 80%, the solid-to-liquid ratio of the longan powder to the ethanol solution is 1:5g/mL, the soaking time is 16-24h, and the rotational speed and the time of centrifugal separation are 4000r/min and 5-10min respectively.
4. The plant polysaccharide-containing feed additive according to claim 2, wherein the volume ratio of the mixed solution a to the distilled water in the step B is 1:15-20, the heating means heating to 80 ℃, and the standing time is 2-3h.
5. The plant polysaccharide-containing feed additive according to claim 2, wherein the solid-to-liquid ratio of the red dates to the absolute ethyl alcohol in the step C is 1:10g/mL, the absolute ethyl alcohol is soaked for 30-60min, and the drying temperature is 40-50 ℃.
6. The plant polysaccharide-containing feed additive according to claim 2, wherein in the step D, the solid-to-liquid ratio of the red date powder to the solvent is 1:5g/mL, the solvent consists of choline chloride and ethylene glycol in a mass ratio of 1:1, the pressurizing pressure is 20-30Mpa, and the ultrasonic treatment time is 20-30min.
7. The plant polysaccharide-containing feed additive according to claim 2, wherein the volume ratio of the mixed solution b to the mixed solution c in the step E is 1:1, and the temperature is raised to 50-60 ℃.
8. The plant polysaccharide-containing feed additive according to claim 1, wherein the preparation method of the plant polysaccharide-containing feed additive comprises the following steps: all the raw material components are mixed to obtain the feed additive containing plant polysaccharide.
9. Use of a plant polysaccharide containing feed additive according to any one of claims 1-8, wherein the plant polysaccharide containing feed additive is applied in fish feed, poultry feed and livestock feed.
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