CN117990430A - Liquid quantitative sampling method and sampling device thereof - Google Patents
Liquid quantitative sampling method and sampling device thereof Download PDFInfo
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- CN117990430A CN117990430A CN202211379249.9A CN202211379249A CN117990430A CN 117990430 A CN117990430 A CN 117990430A CN 202211379249 A CN202211379249 A CN 202211379249A CN 117990430 A CN117990430 A CN 117990430A
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- 239000007788 liquid Substances 0.000 title claims abstract description 177
- 238000005070 sampling Methods 0.000 title claims abstract description 63
- 238000000034 method Methods 0.000 title claims abstract description 21
- 238000006243 chemical reaction Methods 0.000 claims abstract description 131
- 239000012488 sample solution Substances 0.000 claims abstract description 77
- 239000000523 sample Substances 0.000 claims abstract description 56
- 239000003153 chemical reaction reagent Substances 0.000 claims description 54
- 239000002699 waste material Substances 0.000 claims description 28
- 239000012085 test solution Substances 0.000 claims description 20
- 238000004891 communication Methods 0.000 claims description 18
- 239000010902 straw Substances 0.000 claims description 9
- 150000007523 nucleic acids Chemical class 0.000 description 6
- 102000039446 nucleic acids Human genes 0.000 description 6
- 108020004707 nucleic acids Proteins 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 4
- 230000003321 amplification Effects 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- 238000007664 blowing Methods 0.000 description 2
- 239000011888 foil Substances 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- -1 mg2+ Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 239000011535 reaction buffer Substances 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000010723 turbine oil Substances 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
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Abstract
The invention relates to the technical field of medical equipment, in particular to a liquid quantitative sampling method and a liquid quantitative sampling device, wherein the liquid quantitative sampling method comprises the following steps: loading a sample container into a quantitative sampling device, and communicating the sample container with the quantitative connecting pipe fitting; flowing the sample solution in the sample container into and filling a dosing chamber in the dosing connecting tube; and driving the quantitative connecting pipe fitting to move towards the reaction container, so that the quantitative connecting pipe fitting is communicated with the reaction container, and the sample solution in the quantitative cavity flows into the reaction container. The liquid quantitative sampling device introduces and fills the quantitative cavity in the quantitative connecting pipe fitting with the sample solution stored in the sample container through the quantitative connecting pipe fitting, and drives the sample solution in the quantitative cavity to be introduced into the reaction container.
Description
Technical Field
The invention relates to the technical field of medical equipment, in particular to a liquid quantitative sampling method and a liquid quantitative sampling device.
Background
Nucleic acid amplification techniques are common procedures used in molecular biology for the amplification and detection of nucleic acids. Nucleic acid amplification reactions typically require templates, primers, dNTP mixtures, reaction buffers, mg2+, distilled water, and DNA polymerase, and primers typically include various components and materials, such as upstream and downstream primers. In practical operation, the above reactants are added to a PCR reaction tube in a certain proportion, and then the reaction tube is placed in a fluorescence detector for reaction.
Chinese patent CN201310456290.6 discloses an automatic quantitative sampling device for barreled turbine oil, which can set a sampling amount at a controller end, send sampling information to a corresponding device, and start to automatically sample a sample to be sampled; meanwhile, quantitative sampling is realized on the sample by controlling the micro-flowmeter, and the whole sampling process is carried out in a sealed environment, so that the oil sample is prevented from being polluted by the environment, and the accuracy of the oil quality assay is further ensured.
The automatic quantitative sampling device related to the patent needs to be provided with a micro vacuum pump and a micro flowmeter which are respectively used for driving the sampling liquid to enter the liquid and detecting the liquid flow in the sampling tube. The micro vacuum pump is started and stopped, so that the liquid filling error is easy to occur due to control delay, and the sampling capacity is not accurate enough.
In addition, chinese patent CN201811308459.2 discloses a ration formula filling equipment for cosmetics production, and the filling equipment includes cylinder, the right side of cylinder and is located the bottom surface fixed mounting who is equipped with the suction pump of fixed plate, the lower right side intercommunication of suction pump has the drain pipe, the one end and the jar liquid dish intercommunication of suction pump are kept away from to the drain pipe, the one end fixed mounting that the drain pipe is close to the liquid dish has liquid flowmeter, liquid flowmeter's right side and be located the drain pipe and keep away from the one end fixed mounting who is equipped with the solenoid valve of suction pump, the right side of suction pump and be located the bottom surface fixed mounting of fixed plate have the liquid reserve tank, the right-hand member intercommunication of suction pump has the suction pipe, the one end and the bottom intercommunication of liquid reserve tank left surface of suction pump are kept away from to the suction pipe, the positive top intercommunication of liquid reserve tank has the feed liquor mouth. When the filling liquid passes through the liquid flowmeter, the liquid flowmeter can automatically calculate the liquid amount of the bottle body, when the set amount of the liquid flowmeter is reached, the electromagnetic valve can automatically stop, and then the quantitative liquid filled in the liquid container can be automatically filled in the bottle body.
Similarly, in the automatic quantitative sampling device according to the above patent, the liquid flow meter is used to calculate the amount of the liquid to be filled, and the electromagnetic valve is used as a switch for the drain pipe, and when the amount of the liquid to be filled reaches a set value, the electromagnetic valve is closed. In the process, the control delay is easy to occur when the electromagnetic valve is started and stopped, so that the filling amount is error.
To sum up, the existing liquid filling sampling equipment needs to be connected with a liquid flowmeter on a liquid filling pipeline, and the liquid filling capacity is controlled through the liquid flowmeter, but when the water pump is started and stopped, the liquid filling amount is easy to cause liquid filling errors due to control delay, so that the sampling capacity is not accurate enough, and the liquid filling effect is affected.
Disclosure of Invention
The invention aims to provide a quantitative liquid sampling method, which aims to solve the technical problems that the existing liquid filling machine is poor in liquid filling accuracy and the liquid filling capacity cannot be controlled accurately.
The invention aims at providing a liquid quantitative sampling device.
In order to achieve the above object, the present invention further provides a liquid quantitative sampling method, comprising the following steps:
Loading a sample container into a quantitative sampling device, and communicating the sample container with the quantitative connecting pipe fitting;
Flowing the sample solution in the sample container into and filling a dosing chamber in the dosing connecting tube;
And driving the quantitative connecting pipe fitting to move towards the reaction container, so that the quantitative connecting pipe fitting is communicated with the reaction container, and the sample solution in the quantitative cavity flows into the reaction container.
As a further improvement of the invention: the method also comprises the following steps:
Filling test liquid into the reagent tank in the quantitative connecting pipe fitting;
the quantitative connecting pipe fitting is driven to move towards the reaction container, so that the quantitative connecting pipe fitting is communicated with the reaction container, and the test solution in the reagent tank flows into the reaction container.
As a further improvement of the invention: the reaction container is provided with a flow guiding piece which is communicated with the reaction container; the quantitative cavity and the reagent tank are both provided with pistons, and the flow guide piece is used for abutting and opening the pistons so that the quantitative cavity and the reagent tank are both communicated with the reaction container.
As a further improvement of the invention: the method also comprises the following steps:
The elastic piece arranged on the reaction container drives the quantitative connecting pipe fitting to be separated from the reaction container, and part of sample solution and test solution in the reaction container are sucked into the reagent tank;
And repeatedly driving the quantitative connecting pipe fitting to be connected with the reaction container, so that the sample solution and the test solution flow back and forth in the reagent tank and the reaction container, and the sample solution and the test solution are fully and uniformly mixed.
As a further improvement of the invention: the step of flowing the sample solution in the sample container into and filling the dosing chamber in the dosing connecting tube further comprises:
and allowing a part of the sample solution in the sample container to flow into and fill the quantitative cavity, and allowing the other part of the sample solution to flow into the waste liquid groove in the quantitative connecting pipe fitting.
In order to achieve the second object, the present invention provides a liquid quantitative sampling device, comprising:
A sample container;
a reaction vessel;
the quantitative connecting pipe fitting is respectively communicated with the sample container and the reaction container; wherein,
The quantitative connecting pipe fitting is internally provided with a quantitative cavity, and is used for introducing and filling the quantitative cavity with the sample solution stored in the sample container and driving the sample solution in the quantitative cavity to be introduced into the reaction container.
As a further improvement of the invention: the quantitative connecting pipe fitting is characterized in that one end of the quantitative connecting pipe fitting is provided with a liquid inlet, a liquid inlet channel and a waste liquid tank are arranged in the quantitative connecting pipe fitting, and the liquid inlet channel is sequentially communicated with the liquid inlet, the quantitative cavity and the waste liquid tank.
As a further improvement of the invention: the liquid quantitative sampling device also comprises a flow guiding piece, wherein the flow guiding piece is positioned at one end of the reaction container and is communicated with the reaction container; wherein,
One end of the flow guiding piece is connected with a communicating rod, and the communicating rod is connected with the quantitative connecting pipe fitting so that the quantitative connecting pipe fitting is communicated with the reaction container.
As a further improvement of the invention: the communication rod comprises a first communication rod, a piston and a liquid outlet are arranged in the quantitative cavity, the piston is slidable in the quantitative cavity and is attached to the liquid outlet, and the first communication rod is opened and abutted to the piston so that the quantitative cavity is communicated with the reaction container.
As a further improvement of the invention: the communicating rod comprises a second communicating rod, a reagent groove is arranged in the quantitative connecting pipe fitting, and the second communicating rod penetrates through and is connected with the reagent groove so that the reagent groove is communicated with the reaction container.
As a further improvement of the invention: the connecting rod comprises a third connecting rod, the flow guide piece is provided with a liquid outlet communicated with the third connecting rod, a waste liquid tank is arranged in the quantitative connecting pipe fitting, and the third connecting rod penetrates through and is connected with the waste liquid tank so that the waste liquid tank is communicated with the liquid outlet.
As a further improvement of the invention: the quantitative connecting pipe fitting is connected in the shell in a sliding mode, the sample container is connected to one end of the quantitative connecting pipe fitting, the reaction container is fixed to the bottom of the shell, and the quantitative connecting pipe fitting moves towards the reaction container and is connected with the reaction container so that the sample container is communicated with the reaction container.
As a further improvement of the invention: the quantitative connecting pipe fitting is provided with an elastic piece, one end of the elastic piece is connected with the quantitative connecting pipe fitting, and the elastic piece is used for abutting against the reaction container so as to separate the reaction container from the quantitative connecting pipe fitting.
As a further improvement of the invention: the quantitative connecting pipe fitting is provided with a reagent groove, the reaction container is connected with a flow guiding piece communicated with the reagent groove, and the elastic piece is used for abutting against the flow guiding piece so as to separate the flow guiding piece from the reagent groove.
As a further improvement of the invention: one end of the quantitative connecting pipe fitting is connected with a liquid inlet straw, the liquid inlet straw is communicated with the liquid inlet, and the other end of the liquid inlet straw is used for being opened and communicated with the sample container.
Compared with the prior art, the invention has the following beneficial effects:
According to the technical scheme, the sample solution stored in the sample container is introduced through the quantitative connecting pipe fitting and is filled in the quantitative cavity in the quantitative connecting pipe fitting, the sample solution in the quantitative cavity is driven to be introduced into the reaction container, and the filling quantity of each sample solution can be quantitatively controlled due to the fact that the volume of the quantitative cavity is fixed, so that the filling is more accurate.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to the structures shown in these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic view of a quantitative liquid sampling device according to an embodiment of the present application;
FIG. 2 is a schematic diagram illustrating a sampling operation of an embodiment of a quantitative liquid sampling device according to the present application;
FIG. 3 is an exploded view of one embodiment of a liquid quantitative sampling device of the present application;
FIG. 4 is a schematic view of an embodiment of a quantitative connecting tube of the present application;
FIG. 5 is a cross-sectional view of an embodiment of a liquid quantitative sampling device of the present application.
Reference numerals illustrate:
| Reference numerals | Name of the name | Reference numerals | Name of the name |
| 1 | Sample tube | 2 | Reaction tube |
| 3 | Quantitative connecting pipe fitting | 4 | Flow guiding piece |
| 5 | Shell body | 11 | Liquid inlet suction pipe |
| 31 | Quantitative cavity | 32 | Liquid inlet |
| 33 | Liquid inlet channel | 34 | Waste liquid tank |
| 35 | Reagent tank | 36 | Elastic piece |
| 311 | Piston | 312 | Liquid outlet |
| 41 | First connecting rod | 42 | Second communicating rod |
| 43 | Third communicating rod | 44 | Liquid outlet |
| 341 | Liquid discharge tank | 51 | Connecting hole |
| 361 | Spring mounting hole | 45 | Liquid guide needle tube |
The achievement of the objects, functional features and advantages of the present invention will be further described with reference to the accompanying drawings, in conjunction with the embodiments.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It should be noted that, if directional indications (such as up, down, left, right, front, and rear … …) are included in the embodiments of the present invention, the directional indications are merely used to explain the relative positional relationship, movement conditions, etc. between the components in a specific posture, and if the specific posture is changed, the directional indications are correspondingly changed.
In addition, if there is a description of "first", "second", etc. in the embodiments of the present invention, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In addition, if "and/or" and/or "are used throughout, the meaning includes three parallel schemes, for example," a and/or B "including a scheme, or B scheme, or a scheme where a and B are satisfied simultaneously. In addition, the technical solutions of the embodiments may be combined with each other, but it is necessary to base that the technical solutions can be realized by those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should be considered to be absent and not within the scope of protection claimed in the present invention.
The existing liquid filling sampling equipment needs to be connected with a liquid flowmeter on a liquid filling pipeline, and the liquid filling capacity is controlled through the liquid flowmeter, but the liquid filling amount is easy to occur due to the control delay when the water pump is started and stopped by the control mode, so that the sampling capacity is not accurate enough, and the liquid filling effect is affected.
The invention aims to provide a liquid quantitative sampling device, and aims to solve the technical problems that the existing liquid filling machine is poor in liquid filling accuracy and the liquid filling capacity cannot be controlled accurately.
Referring to fig. 1 to 5, in an embodiment of the quantitative liquid sampling device of the present application, a sample container is specifically a sample tube 1, and a reaction container is specifically a reaction tube 2. The liquid quantitative sampling device is used for integrated nucleic acid detection, and is matched with ultraRADAR sample virus treatment system and ultraRADAR nucleic acid detection reagent.
The liquid quantitative sampling device of the present embodiment includes:
a sample tube 1;
a reaction tube 2;
A quantitative connecting pipe fitting 3, wherein the quantitative connecting pipe fitting 3 is respectively communicated with the sample tube 1 and the reaction tube 2; wherein,
A quantifying cavity 31 is arranged in the quantifying connecting pipe fitting 3, and the quantifying connecting pipe fitting 3 is used for introducing and filling the quantifying cavity 31 with the sample solution stored in the sample tube 1, and driving the sample solution in the quantifying cavity 31 to be introduced into the reaction tube 2.
Specifically, in the present embodiment, the sample tube 1 is used for storing a sample solution, and the quantitative connection tube 3 is used for introducing the sample solution into the reaction tube 2. When the sample tube 1 and the quantitative connecting tube 3 are installed, the aluminum foil used for sealing at the top of the sample tube 1 is punctured, and one end of the quantitative connecting tube 3 extends into the sample tube 1. The sample solution in the sample tube 1 is pressed into the quantitative cavity 31 in the quantitative connecting tube 3 due to the air pressure, and the sample solution in the quantitative cavity 31 is introduced into the reaction tube 2 under the action of external driving force due to the communication of the quantitative connecting tube 3 with the reaction tube 2, thereby realizing the transfer of the sample solution. In addition, since the volume of the quantitative chamber 31 is fixed, the volume of the sample solution introduced into the reaction tube 2 is uniform every time the drive is performed, thereby realizing quantitative control of the filling amount of the sample solution.
According to the technical scheme, the sample solution stored in the sample tube 1 is introduced into and fills the quantitative cavity 31 in the quantitative connecting tube through the quantitative connecting tube 3, and the sample solution in the quantitative cavity 31 is driven to be introduced into the reaction tube 2, and the filling quantity of each sample solution can be quantitatively controlled due to the fixed volume of the quantitative cavity 31, so that the filling is more accurate.
Further, in one embodiment, a liquid inlet 32 is disposed at one end of the quantitative connection pipe 3, a liquid inlet channel 33 and a waste liquid tank 34 are disposed in the quantitative connection pipe 3, and the liquid inlet channel 33 is sequentially connected with the liquid inlet 32, the quantitative cavity 31 and the waste liquid tank 34.
Specifically, referring to fig. 3 and 4, in the present embodiment, after the sample enters the liquid inlet channel 33 of the quantifying connecting tube 3, the sample solution flows toward the quantifying cavity 31, and the sample solution first passes through the quantifying cavity 31, and then flows into the quantifying cavity under the action of gravity and completely fills the quantifying cavity 31. The volume of the dosing chamber 31 is the volume of the dosing solution. Meanwhile, a liquid outlet 312 is provided at the bottom of the dosing chamber 31, but since the liquid outlet 312 is sealed with an aluminum foil, the sample solution does not flow out of the dosing chamber. After the quantitative chamber 31 is completely filled, the excess sample solution continues to flow toward the waste liquid tank 34 and finally flows into the waste liquid tank 34.
Further, in one embodiment, the quantitative liquid sampling device further comprises a flow guiding member 4, wherein the flow guiding member 4 is positioned at one end of the reaction tube 2 and is communicated with the reaction tube 2; wherein,
One end of the flow guiding piece 4 is connected with a communicating rod, and the communicating rod is connected with the quantitative connecting pipe fitting 3, so that the quantitative connecting pipe fitting 3 is communicated with the reaction pipe 2.
Specifically, in the present embodiment, the flow guide 4 is used to pierce the reaction tube 2 and communicate with the reaction tube 2 by the bottom of the flow guide 4 pushed by the quantitative connection tube 3 when the quantitative connection tube 3 moves toward the reaction tube 2 and connects the reaction tube 2. The top of the flow guiding piece 4 is provided with a communicating rod, and the communicating rod is used for connecting the quantitative connecting pipe fitting 3, so that the sample solution of the quantitative cavity 31 in the quantitative connecting pipe fitting 3 flows through the flow guiding piece 4 and then flows into the reaction pipe 2.
Further, in an embodiment, the communicating rod includes a first communicating rod 41, a piston 311 and a liquid outlet 312 are disposed in the dosing chamber 31, the piston 311 is slidable in the dosing chamber 31 and fits the liquid outlet 312, and the first communicating rod 41 is opened and abuts against the piston 311, so that the dosing chamber 31 is communicated with the reaction tube 2.
Specifically, in the present embodiment, the first communication lever 41 is disposed vertically, and the inside is hollow. The liquid outlet 312 of the quantitative cavity 31 is disposed at the bottom of the quantitative cavity 31, and the piston 311 can slide up and down in the quantitative cavity 31, so that the liquid outlet 312 can be attached under the action of gravity, and the sample solution in the cavity is prevented from flowing out. When the dosing tube 3 moves toward the reaction tube 2, the dosing chamber 31 in the dosing tube 3 is in contact with the first communication rod 41. At this time, the first communicating rod 41 is abutted upward against the piston from the bottom of the dosing chamber 31, the piston is separated from the liquid outlet, and the sample solution flows into the first communicating rod 31 and then into the reaction tube 2.
Further, in an embodiment, the communicating rod includes a second communicating rod 42, the reagent tank 35 is disposed in the quantitative connecting tube 3, and the second communicating rod 42 penetrates the reagent tank 35 and is connected to the reagent tank 35, so that the reagent tank 35 communicates with the reaction tube 2.
Specifically, referring to fig. 4, in the present embodiment, a reagent tank 35 is disposed at one side of the quantifying chamber 31, and the reagent tank 35 is filled with a test solution for activating the sample solution. A liquid outlet and a slidable piston having the same structure as the quantitative chamber 31 are provided in the reagent tank 35. The second communicating rod 42 is hollow, when the quantitative connecting pipe fitting 3 moves towards the reaction tube 2, the second communicating rod 42 contacts with the reagent tank 35, the second communicating rod 42 is upwards abutted against the piston from the bottom of the reagent tank 35, the piston is separated from the liquid outlet, the test liquid flows into the second communicating rod 42, and then uniformly flows into the reaction tube 2 together with the sample solution.
Referring to fig. 5, a liquid guiding needle tube 45 is connected to the bottom of the guiding element 4. The liquid guide needle tube 45 is used for puncturing the sealing film of the reaction tube 2, so that the flow guide piece 4 is communicated with the quantitative cavity 31 and the reagent groove 35. Specifically, the first communicating rod 41 and the second communicating rod 42 are hollow, and the flow guiding member 4 is provided with a through hole, so that the sample solution flows through the first communicating rod 41, the test solution flows through the second communicating rod 42, and the two liquids are converged to enter the through hole of the flow guiding member 4 and then enter the reaction tube 2 at the same time.
Further, in one embodiment, the communicating rod includes a third communicating rod 43, the fluid guiding member 4 is provided with a fluid outlet 44 communicating with the third communicating rod, the waste liquid tank 34 is disposed in the quantitative connecting tube 3, and the third communicating rod 43 penetrates the waste liquid tank 34 and is connected to the waste liquid tank 34, so that the waste liquid tank 34 communicates with the fluid outlet 44.
Specifically, referring to FIG. 4, in the present embodiment, the waste liquid tank 34 includes a liquid discharge tank 341, the waste liquid tank 34 is communicated with the liquid discharge tank 341 through a liquid inlet pipe 33, the liquid discharge tank 341 is provided at one side of the reagent tank 35, and when the sample solution stored in the waste liquid tank 34 is saturated, the sample solution flows from the waste liquid tank 34 into the liquid discharge tank 341. When the quantitative connection pipe 3 moves toward the reaction tube 2, the third communication rod 43 is connected to the liquid discharge groove 341, and the sample solution flows into the third communication rod 43 and then flows to the outside of the apparatus through the liquid discharge port 44.
Further, in one embodiment, the quantitative liquid sampling device further comprises a housing 5, the quantitative connection tube 3 is slidably connected in the housing 5, the sample tube 1 is connected to one end of the quantitative connection tube 3, the reaction tube 2 is fixed to the bottom of the housing 5, and the quantitative connection tube 3 moves towards the reaction tube 2 and is connected to the reaction tube 2, so that the sample tube 1 is in communication with the reaction tube 2.
Specifically, in the present embodiment, the top of the housing 5 is provided with a connection hole 51, the housing 5 is installed in the nucleic acid detecting integrated machine, the equipment is extended into the connection hole 51 through a link (not shown) which is connected with the quantitative connecting tube 3, and then the movement of the quantitative connecting tube 3 is controlled by a cylinder or a motor. Because the reaction tube 2 is fixed at the bottom of the shell 5, when the device drives the quantitative connection pipe fitting 3 to move downwards, the quantitative connection pipe fitting 3 is connected with the reaction tube 2, so that the communication between the sample tube 1 and the reaction tube 2 is realized.
Further, in an embodiment, the quantitative connection tube 3 is provided with an elastic member 36, one end of the elastic member 36 is connected to the quantitative connection tube 3, and the elastic member 36 is used for abutting against the reaction tube 2 to separate the reaction tube 2 from the quantitative connection tube 3.
Specifically, referring to fig. 3, in the present embodiment, the elastic member 36 is a spring. Spring mounting holes 361 are provided on both sides of the reagent tank 35, and the elastic member 36 is provided in the spring mounting holes 361. When the quantitative connecting tube 3 moves toward the reaction tube 2, one end of the elastic member 36 abuts against the flow guiding member 4, and at this time, the elastic member 36 is compressed. When the driving force of the quantitative connection tube 3 is removed, the elastic force of the elastic member 36 acts on the flow guide member 4, and the quantitative connection tube 3 is separated from the reaction tube 2. At this time, the piston in the reagent vessel 35 is sprung back by the elastic force, and the piston rises, so that a part of the sample solution and the test solution is withdrawn from the reaction tube 2 into the reagent vessel 35. Through repeated reciprocating drive ration connecting tube 3, simulate the effect of blowing mixing for sample solution and test solution round trip flow, thereby realize test solution and sample solution homogeneous mixing.
Further, in one embodiment, the quantitative connection tube 3 is provided with a reagent tank 35, the reaction tube 2 is connected with a flow guiding member 4 that is in communication with the reagent tank 35, and the elastic member 36 is used for abutting against the flow guiding member 4 so as to separate the flow guiding member 4 from the reagent tank 35.
Specifically, in the present embodiment, the elastic member 36 is used to abut against the flow guiding member 4. When the driving force of the quantitative connection tube 3 is removed, the elastic force of the elastic member 36 acts on the flow guide 4, thereby separating the flow guide 4 from the reagent tank 35.
Further, in an embodiment, one end of the quantitative connection pipe 3 is connected with a liquid inlet straw 11, the liquid inlet straw 11 is communicated with the liquid inlet 32, and the other end of the liquid inlet straw 11 is used for opening and communicating with the sample tube 1, so that the sample solution in the sample tube 1 enters the quantitative connection pipe 3.
In summary, the liquid quantitative sampling device can transfer the sample solution into the quantitative cavity without a separate power source, and quantitatively pour the sample solution into the reaction tube 2 by driving the quantitative connecting tube 3.
The invention also provides a liquid quantitative sampling method, which adopts the liquid quantitative sampling device of the embodiment, and comprises the following steps:
S100: loading a sample tube into a quantitative sampling device, and communicating the sample tube with the quantitative connecting pipe fitting;
s200: flowing the sample solution in the sample tube into and filling the dosing chamber in the dosing connecting tube;
s300: and driving the quantitative connecting pipe fitting to move towards the reaction pipe, so that the quantitative connecting pipe fitting is communicated with the reaction pipe, and the sample solution in the quantitative cavity flows into the reaction pipe.
Specifically, in the present embodiment, since the quantitative connection tube member introduces and fills the quantitative chamber in the quantitative connection tube member with the sample solution stored in the sample tube, the sample solution in the quantitative chamber is introduced into the reaction tube by driving. And the volume of the quantitative cavity is fixed, so that the filling quantity of each sample solution can be ensured, and the filling is more accurate.
Further, in an embodiment, the liquid quantitative sampling method further comprises the steps of:
S400: filling test liquid into the reagent tank in the quantitative connecting pipe fitting;
S500: the quantitative connecting pipe fitting is driven to move towards the reaction pipe, so that the quantitative connecting pipe fitting is communicated with the reaction pipe, and the test liquid in the reagent tank flows into the reaction pipe.
Specifically, in this embodiment, the quantitative connection pipe is driven to move toward the reaction tube, and at this time, the quantitative connection pipe is connected to the reaction tube, and the reagent tank and the quantitative chamber are respectively connected to the reaction tube, so that the test solution in the reagent tank and the sample solution in the quantitative chamber flow into the reaction tube at the same time.
Further, in one embodiment, the reaction tube is provided with a flow guiding member, and the flow guiding member is communicated with the reaction tube; the quantitative cavity and the reagent tank are both provided with pistons, and the flow guide piece is used for abutting and opening the pistons so that the quantitative cavity and the reagent tank are both communicated with the reaction tube.
Further, in an embodiment, the liquid quantitative sampling method further comprises the steps of:
The elastic piece arranged on the reaction tube drives the quantitative connecting pipe fitting to be separated from the reaction tube, and part of sample solution and test solution in the reaction tube are sucked into the reagent tank;
And repeatedly driving the quantitative connecting pipe fitting to be connected with the reaction pipe, so that the sample solution and the test solution flow back and forth in the reagent tank and the reaction pipe, and the sample solution and the test solution are fully and uniformly mixed.
Specifically, in the present embodiment, when the quantitative connecting tube is separated from the reaction tube, a part of the sample solution and the test solution is withdrawn from the reaction tube into the reagent vessel. The quantitative connecting pipe fitting is connected and separated with the reaction pipe by repeatedly driving the quantitative connecting pipe fitting for a plurality of times, so that the effect of blowing and mixing is simulated, the sample solution and the test solution flow back and forth in the reagent tank and the reaction pipe, and the uniform mixing of the test solution and the sample solution is realized.
Further, in an embodiment, the step S200: flowing the sample solution in the sample tube into and filling the dosing chamber in the dosing connecting tube further comprises:
And allowing one part of the sample solution in the sample tube to flow into and fill the quantitative cavity, and allowing the other part of the sample solution to flow into the waste liquid groove in the quantitative connecting pipe fitting.
Specifically, in this embodiment, after the sample solution flows into the quantitative chamber, since the volume of the quantitative chamber is fixed, the excess sample solution flows into the waste liquid tank to be stored, and when the waste liquid tank is full of the sample solution, the sample solution is discharged out of the apparatus through the liquid discharge port.
The foregoing description is only of the optional embodiments of the present invention, and is not intended to limit the scope of the invention, and all the equivalent structural changes made by the description of the present invention and the accompanying drawings or the direct/indirect application in other related technical fields are included in the scope of the invention.
Claims (15)
1. A method for quantitatively sampling a liquid, comprising the steps of:
Loading a sample container into a quantitative sampling device, and communicating the sample container with the quantitative connecting pipe fitting;
Flowing the sample solution in the sample container into and filling a dosing chamber in the dosing connecting tube;
And driving the quantitative connecting pipe fitting to move towards the reaction container, so that the quantitative connecting pipe fitting is communicated with the reaction container, and the sample solution in the quantitative cavity flows into the reaction container.
2. The method of quantitatively sampling a liquid according to claim 1, further comprising the steps of:
Filling test liquid into the reagent tank in the quantitative connecting pipe fitting;
the quantitative connecting pipe fitting is driven to move towards the reaction container, so that the quantitative connecting pipe fitting is communicated with the reaction container, and the test solution in the reagent tank flows into the reaction container.
3. The quantitative liquid sampling method according to claim 2, wherein a flow guiding member is provided on the reaction vessel, and the flow guiding member is communicated with the reaction vessel; the quantitative cavity and the reagent tank are both provided with pistons, and the flow guide piece is used for abutting and opening the pistons so that the quantitative cavity and the reagent tank are both communicated with the reaction container.
4. The method of quantitatively sampling a liquid according to claim 2, further comprising the steps of:
The elastic piece arranged on the reaction container drives the quantitative connecting pipe fitting to be separated from the reaction container, and part of sample solution and test solution in the reaction container are sucked into the reagent tank;
And repeatedly driving the quantitative connecting pipe fitting to be connected with the reaction container, so that the sample solution and the test solution flow back and forth in the reagent tank and the reaction container, and the sample solution and the test solution are fully and uniformly mixed.
5. The method of claim 1, wherein the step of flowing the sample solution in the sample container into and filling the dosing chamber in the dosing tube further comprises:
and allowing a part of the sample solution in the sample container to flow into and fill the quantitative cavity, and allowing the other part of the sample solution to flow into the waste liquid groove in the quantitative connecting pipe fitting.
6. A liquid quantitative sampling device, comprising:
A sample container;
a reaction vessel;
the quantitative connecting pipe fitting is respectively communicated with the sample container and the reaction container; wherein,
The quantitative connecting pipe fitting is internally provided with a quantitative cavity, and is used for introducing and filling the quantitative cavity with the sample solution stored in the sample container and driving the sample solution in the quantitative cavity to be introduced into the reaction container.
7. The liquid quantitative sampling device according to claim 6, wherein a liquid inlet is formed in one end of the quantitative connecting pipe, a liquid inlet channel and a waste liquid tank are formed in the quantitative connecting pipe, and the liquid inlet channel is sequentially communicated with the liquid inlet, the quantitative cavity and the waste liquid tank.
8. The liquid quantitative sampling device according to claim 6, further comprising a flow guide member located at one end of the reaction vessel and in communication with the reaction vessel; wherein,
One end of the flow guiding piece is connected with a communicating rod, and the communicating rod is connected with the quantitative connecting pipe fitting so that the quantitative connecting pipe fitting is communicated with the reaction container.
9. The liquid quantitative sampling device according to claim 8, wherein the communication rod comprises a first communication rod, a piston and a liquid outlet are arranged in the quantitative cavity, the piston is slidable in the quantitative cavity and is attached to the liquid outlet, and the first communication rod is opened and abuts against the piston to enable the quantitative cavity to be communicated with the reaction container.
10. The liquid quantitative sampling device according to claim 8, wherein the communication rod comprises a second communication rod, a reagent tank is arranged in the quantitative connection pipe fitting, and the second communication rod penetrates through and is connected with the reagent tank so that the reagent tank is communicated with the reaction container.
11. The liquid quantitative sampling device according to claim 8, wherein the communicating rod comprises a third communicating rod, the flow guiding member is provided with a liquid outlet communicated with the third communicating rod, the quantitative connecting pipe is internally provided with a waste liquid tank, and the third communicating rod penetrates through and is connected with the waste liquid tank so as to enable the waste liquid tank to be communicated with the liquid outlet.
12. The liquid quantitative sampling device according to claim 6, further comprising a housing, wherein the quantitative connecting pipe is slidably connected in the housing, wherein the sample container is connected to one end of the quantitative connecting pipe, wherein the reaction container is fixed to a bottom of the housing, and wherein the quantitative connecting pipe moves toward and is connected to the reaction container so that the sample container communicates with the reaction container.
13. The liquid quantitative sampling device according to claim 12, wherein the quantitative connection pipe is provided with an elastic member, one end of the elastic member is connected with the quantitative connection pipe, and the elastic member is used for abutting against the reaction container so as to separate the reaction container from the quantitative connection pipe.
14. The liquid quantitative sampling device according to claim 13, wherein the quantitative connection pipe is provided with a reagent tank, a flow guiding member communicated with the reagent tank is connected to the reaction container, and the elastic member is used for abutting against the flow guiding member so as to separate the flow guiding member from the reagent tank.
15. The liquid quantitative sampling device according to claim 7, wherein one end of the quantitative connection pipe is connected with a liquid inlet straw, the liquid inlet straw is communicated with the liquid inlet, and the other end of the liquid inlet straw is used for opening and communicating with the sample container.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211379249.9A CN117990430A (en) | 2022-11-04 | 2022-11-04 | Liquid quantitative sampling method and sampling device thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211379249.9A CN117990430A (en) | 2022-11-04 | 2022-11-04 | Liquid quantitative sampling method and sampling device thereof |
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| Publication Number | Publication Date |
|---|---|
| CN117990430A true CN117990430A (en) | 2024-05-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202211379249.9A Pending CN117990430A (en) | 2022-11-04 | 2022-11-04 | Liquid quantitative sampling method and sampling device thereof |
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| Country | Link |
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| CN (1) | CN117990430A (en) |
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- 2022-11-04 CN CN202211379249.9A patent/CN117990430A/en active Pending
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