CN117918175A - Method for preventing and controlling bactrocera dorsalis in peach orchard and bactrocera dorsalis preventing and controlling attractant - Google Patents

Method for preventing and controlling bactrocera dorsalis in peach orchard and bactrocera dorsalis preventing and controlling attractant Download PDF

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CN117918175A
CN117918175A CN202410066451.9A CN202410066451A CN117918175A CN 117918175 A CN117918175 A CN 117918175A CN 202410066451 A CN202410066451 A CN 202410066451A CN 117918175 A CN117918175 A CN 117918175A
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bactrocera dorsalis
controlling
insect
peach
preventing
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王轶
沈建生
吴嘉颀
孙萍
林贤锐
朱建锡
陈辰斐
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Zhejiang Agricultural Machinery Research Institute
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Zhejiang Agricultural Machinery Research Institute
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/40Fabaceae, e.g. beans or peas
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01MCATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M1/00Stationary means for catching or killing insects
    • A01M1/02Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01MCATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M1/00Stationary means for catching or killing insects
    • A01M1/14Catching by adhesive surfaces
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/10Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00Arthropodicides
    • A01P7/04Insecticides

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Insects & Arthropods (AREA)
  • General Health & Medical Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Catching Or Destruction (AREA)

Abstract

The invention provides a method for preventing and controlling bactrocera dorsalis in a peach garden and an bactrocera dorsalis preventing and controlling attractant, and aims to solve the problem that the preventing and controlling effect on bactrocera dorsalis in the peach garden is not ideal in the prior art. The method for preventing and controlling the bactrocera dorsalis in the peach orchard comprises the following steps: s100, intensively cleaning worm disease fruits; s200, bagging the fruits; s300, sowing or broadcasting the astragalus sinicus seeds in autumn and winter; s400, setting a trap or an insect-attracting plate in 6-10 months, wherein n-octyl phthalate and/or 3-isobutyl hexahydropyrrole [1,2-a ] pyrazine-1, 4-dione are added into the trap or the insect-attracting core of the insect-attracting plate. Because the milk vetch has better repellent effect on the bactrocera dorsalis, the bactrocera dorsalis is easier to approach to the n-octyl phthalate and 3-isobutyl hexahydropyrrole [1,2-a ] pyrazine-1, 4-dione trapping device which have volatility and attract the bactrocera dorsalis; the milk vetch and the trapping device containing n-octyl phthalate and 3-isobutyl hexahydro pyrrole [1,2-a ] pyrazine-1, 4-dione can better realize the effect of preventing the bactrocera dorsalis.

Description

Method for preventing and controlling bactrocera dorsalis in peach orchard and bactrocera dorsalis preventing and controlling attractant
Technical Field
The invention belongs to the technical field of control of bactrocera dorsalis in peach orchards, and particularly relates to a control method and a control attractant for bactrocera dorsalis in peach orchards.
Background
The sex attractant, yellow board and other tools are utilized to select representative peach gardens in the peach producing area in Jinhua to carry out investigation and research on the occurrence characteristics and occurrence dynamics of peach garden fruit moth pests such as carpopodium borer, fruit moth, fruit fly, carpopodium borer and the like; the investigation shows that the fruit-eating pests of the peach nurseryman in the kumquat area are fruit flies and fruit borers, and the quantity and the hazard degree of the fruit flies are higher than those of the fruit borers. The carpopodium borer, the fruit-sucking noctuid and the carpopodium borer are also found in the Jinhua area, but the hazard degree is low. However, the current farmers have an unsatisfactory control effect on the bactrocera dorsalis in the peach orchard.
Disclosure of Invention
The invention provides a method for preventing and controlling bactrocera dorsalis in a peach garden and an bactrocera dorsalis preventing and controlling attractant, and aims to solve the problem that the preventing and controlling effect on bactrocera dorsalis in the peach garden is not ideal in the prior art.
In order to solve the technical problems, the invention adopts the following technical scheme:
in a first aspect, the invention provides a method for controlling bactrocera dorsalis in a peach orchard, which comprises the following steps:
S100, cleaning an orchard: intensively cleaning the insect disease fruits;
s200, fruit bagging: bagging the fruits;
S300, sowing or broadcasting the astragalus sinicus seeds in autumn and winter;
s400, setting a trap or an insect-attracting plate in 6-10 months, wherein n-octyl phthalate and/or 3-isobutyl hexahydropyrrole [1,2-a ] pyrazine-1, 4-dione are added into the trap or the insect-attracting core of the insect-attracting plate.
The further improved scheme is as follows: in step S100, during the fruit ripening period, the insects on the tree are removed periodically, the fruits falling on the ground are picked up, and the fruits are put into the insects treatment bag for centralized treatment of the insects.
Based on the scheme, the insect fruit processing bag is used for centralized processing to replace the deep-buried insect fruit, so that the fruit fly can be eradicated from the insect fruit.
The further improved scheme is as follows: in the step S200, bagging is carried out 30-45 days after flowers, redundant leaves on the fruit stalks or the fruit cluster bases are removed before bagging, and meanwhile, 1 time of pesticide and bactericide are sprayed; the bag opening is tied up when the bag is sleeved, and the bottom is provided with a hole for ventilation.
Based on the scheme, the principle of bagging is to wrap fruits so as to prevent the fruit fly from entering the fruits to lay eggs, and the fruit retention rate can reach more than 97%.
The further improved scheme is as follows: in the step S300, the seed quantity of the milk vetch seeds is 45 kg-75 kg/hm 2.
Based on the scheme, the seed quantity is adopted, the astragalus sinicus grows vigorously in spring, and the rows can be covered in the spring flowering phase, so that the weeds in the spring peach orchard are controlled, and the inhibition effect on malignant weeds between the orchards is achieved.
The further improved scheme is as follows: in the step S400, an insect attracting plate is arranged in 6-10 months, is hung on the position, on the upper part of the peach tree, of the downwind position in the back, direct sunlight is avoided, 300-375 pieces/hm 2 is avoided, and after the insect body is adhered, the insect attracting plate is replaced in time and is hung for 2 times continuously.
Based on the scheme, the insect attracting plate is hung on the downwind position of the upper back of the upper branch of the peach tree, so that the service life of the insect attracting plate can be prolonged.
The further improved scheme is as follows: in step S400, the suspension of 40-80/hm 2 traps is set at 6-10 months.
Based on the above scheme, the number of traps can be selected according to the severity of the occurrence of the bactrocera dorsalis.
The further improved scheme is as follows: the method for preventing and controlling the bactrocera dorsalis in the peach orchard further comprises the following steps: and a sexual attractant is added into the attracting cores of part of the traps or the attracting plates.
Based on the scheme, sex attractant is utilized to trap and kill a large amount of male insects, so that the probability of mating male and female insects in the field is reduced, and the density of insect population of the next generation is reduced.
The further improved scheme is as follows: the method for preventing and controlling the bactrocera dorsalis in the peach orchard further comprises the steps of raising chickens and killing insects: raising chickens in peach orchards, and prohibiting pesticide use in orchards during the period of raising chickens.
Based on the scheme, chickens are raised in the peach orchard to eat the bactrocera dorsalis pupae in the surface soil, and the breeding coefficient of pests in the next generation can be reduced by pecking the bactrocera dorsalis larvae in the ground fruits.
The further improved scheme is as follows: the method for preventing and controlling the bactrocera dorsalis in the peach orchard further comprises the following steps: and releasing parasitic wasps in the field.
Based on the scheme, for a peach garden with good ecology, natural enemy parasitic wasps of the bactrocera dorsalis are released, and the effect of controlling the bactrocera dorsalis naturally can be fully exerted.
In a second aspect, the invention provides a bactrocera dorsalis control attractant comprising n-octyl phthalate and/or 3-isobutyl hexahydropyrrole [1,2-a ] pyrazine-1, 4-dione.
The beneficial effects of the invention are as follows:
According to the invention, experiments prove that the main metabolites of the peach are higher in attraction rate of n-octyl phthalate and 3-isobutyl hexahydro-pyrrole [1,2-a ] pyrazine-1, 4-dione to the bactrocera dorsalis, and the main metabolites can be used in a trap or an attractant of an insect attracting plate for attracting the bactrocera dorsalis, so that the effect of preventing and controlling the bactrocera dorsalis is achieved.
In addition, experiments prove that the milk vetch has better repellent effect on the bactrocera dorsalis; because the milk vetch has better repellent effect on the bactrocera dorsalis, the bactrocera dorsalis is easier to approach to the n-octyl phthalate and 3-isobutyl hexahydropyrrole [1,2-a ] pyrazine-1, 4-dione trapping device which have volatility and attract the bactrocera dorsalis; the milk vetch and the trapping device containing n-octyl phthalate and 3-isobutyl hexahydro pyrrole [1,2-a ] pyrazine-1, 4-dione can better realize the effect of preventing the bactrocera dorsalis.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a diagram of planting different flowering plants in a peach orchard.
FIG. 2 is a graph showing the comparison of the number of Bactrocera dorsalis after planting different flowering plants.
FIG. 3 is a sample volatile material profile.
FIG. 4 is a statistical chart of the number of compounds detected by the sample.
FIG. 5 is a statistical chart of the number of samples detected for each compound
FIG. 6 is a graph comparing the behavioral response of bactrocera dorsalis to 13 chemicals.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention. All other embodiments, which can be obtained by a person skilled in the art without creative efforts, are included in the protection scope of the present invention based on the embodiments of the present invention.
Embodiment one:
The embodiment provides a method for preventing and controlling Bactrocera dorsalis in peach orchard, which comprises the following steps:
S100, cleaning an orchard: intensively cleaning the insect disease fruits;
specifically, during the fruit ripening period, the insects on the tree are removed periodically, the fruits falling on the ground are picked up, and the fruits are put into the insects treatment bag for centralized treatment of the insects.
The insect fruit processing bags are used for replacing the deep-buried insect fruits in the pits, 75 insect fruit processing bags per hm 2 are configured and used for picking up fallen fruits and rotten fruits, the insect disease fruits are processed in a concentrated mode, and insect sources are reduced. During the period of peach fruit ripening, picking up the insects on the tree at regular intervals (1 time per week), picking up the fruits falling on the ground (picking up the fruits falling 1 time per day from the full period to the final period of the fruits falling), putting the insects, the fruits falling and the like into the insect-fruit processing bag without dead angles, intensively processing the insects, and killing the larvae and the pupae in different development periods, thereby reducing the density of insect population in the field.
S200, fruit bagging: bagging the fruits;
Specifically, the bagging time is 30-45 days after flowers, redundant leaves on the fruit stalks or the base parts of the clusters are removed before bagging, and meanwhile, the insecticidal and bactericidal agent is sprayed for 1 time; the bag opening is tied up when the bag is sleeved, and the bottom is provided with a hole for ventilation.
Meanwhile, the selection of the types of fruit bags is particularly important, and fruit bags with good texture, light transmittance and strong air permeability are generally selected; the bagging is carried out on sunny days.
The principle of the bagging is that fruit is wrapped to prevent fruit fly from entering the fruit to lay eggs, and the fruit retention rate can reach more than 97%.
S300, sowing or broadcasting the astragalus sinicus seeds in autumn and winter;
Specifically, the seed quantity of the milk vetch seeds is 45 kg-75 kg/hm 2.
In autumn and winter (in middle and late 10 months), according to the soil moisture content of the peach orchard, grass seeds such as milk vetch and the like are sown or broadcasted, and the seed consumption is 45 kg-75 kg/hm 2. The warm climate and rainwater in autumn and winter are utilized to promote germination and growth of cold season grass seeds, after a freezing process in winter, the spring vigor is vigorous, and the spring vigor can cover the inter-row weeds in the spring flowering period, so that the weeds in the spring peach orchard are controlled, and the weed control agent has an inhibition effect on the inter-garden malignant weeds. And Mao She seeds which grow more vigorously on the tree plates are cut after flowering, so that climbing of the tree is prevented, permeability among flowers is kept, and grass cushion layers are formed under the tree after pod bearing is naturally dead, so that management among the flowers is facilitated.
S400, setting a trap or an insect-attracting plate in 6-10 months, wherein n-octyl phthalate and/or 3-isobutyl hexahydropyrrole [1,2-a ] pyrazine-1, 4-dione are added into the trap or the insect-attracting core of the insect-attracting plate.
Wherein, trappers (lure worm cover, sex lure bucket etc.) or lure worm board can purchase according to actual demand, for example: insect attracting plate (double-sided 20 x 100cm sticky tape; blue double-sided 25 x 20cm, blue double-sided 25 x 40cm, huang Shuangmian x 20cm, huang Shuangmian x 40cm, green double-sided 25 x 20cm sticky plate); insect attracting cover (containing sugar vinegar solution, patent number: ZL 202023081248.5), sex attractant bucket (Nanjing Xin Zhongqing Biotechnology Co., ltd.); only n-octyl phthalate and/or 3-isobutyl hexahydropyrrole [1,2-a ] pyrazine-1, 4-dione are added into the trap or the trap core of the trap plate; under the condition of no conflict, sex attractants and other attractants capable of attracting the bactrocera dorsalis can be added into the lure; the addition amount of the n-octyl phthalate and/or the 3-isobutyl hexahydro pyrrole [1,2-a ] pyrazine-1, 4-dione can be added according to different specifications of traps and insect trap plates and actual conditions.
Specifically, in step S400, an insect attracting plate is arranged in 6-10 months, the insect attracting plate is hung on the position of the upper part of the peach tree, which is downwind from the back, so that direct sunlight is avoided, 300-375 pieces/hm 2 is avoided, and the insect attracting plate is replaced in time after being stuck with insects and is hung for 2 times continuously.
Or in step S400, the suspension of 40-80/hm 2 traps is set at 6-10 months. In areas where the bactrocera dorsalis occurs seriously, 75/hm 2 traps are hung; in the slight region, the bactrocera dorsalis only needs to be hung by 45 bactrocera dorsalis per hm 2. 1.5-2 mL of the liquid is added into each trapper at one time, 1 time is added every 30-40 d, and the trapper is cleaned in time.
On the basis of any scheme, the method for preventing and controlling the bactrocera dorsalis in the peach orchard further comprises the following steps: and a sexual attractant is added into the attracting cores of part of the traps or the attracting plates.
On the basis of any scheme, the method for preventing and controlling the bactrocera dorsalis in the peach orchard further comprises the steps of raising chickens and killing insects: raising chickens in peach orchards, and prohibiting pesticide use in orchards during the period of raising chickens.
Specifically, mature larvae enter surface soil pupation through bouncing from fallen fruits, chickens can be raised in peach orchards by utilizing the phenomenon to pick up the bactrocera dorsalis pupae in the surface soil, and the insect breeding coefficient of the next generation can be reduced by pecking up the bactrocera dorsalis larvae in fallen fruits.
On the basis of any scheme, the method for preventing and controlling the bactrocera dorsalis in the peach orchard further comprises the following steps: and releasing parasitic wasps in the field.
Specifically, for a peach garden with good ecology, natural enemies of the bactrocera dorsalis are released, and the natural control effect of the bactrocera dorsalis can be fully exerted. Under the general condition, the cardinality of the bactrocera dorsalis is firstly depressed, then the parasitic wasps in different insect states such as egg parasitic wasps, larva parasitic wasps, pupa parasitic wasps and the like are released in the field according to the field monitoring condition, and the release quantity of the parasitic wasps is determined according to the population quantity of the bactrocera dorsalis in the field. The bee laying condition needs to meet the clear weather, the wind power is less than 3 levels, the air temperature is above 22 ℃, and the time is generally 10:00-16:00 better.
Embodiment two:
the embodiment provides a bactrocera dorsalis control attractant, which comprises n-octyl phthalate and/or 3-isobutyl hexahydropyrrole [1,2-a ] pyrazine-1, 4-dione. Reference is made to embodiment one for specific use.
The invention is further illustrated in the following in connection with the test:
test one: research on regulating and controlling effects of astragalus sinicus on fruit-eating pests in peach orchard
1. Materials and methods
1.1 Test floor
The experimental plot was jinhua city source east country east forward village.
1.2 Test methods
The peach tree species are flat peaches of golden nepheline, each species is interplanted with 90 square meters, and about 8-10 peach trees are interplanted. Referring to the similar fruit tree grass planting method, combined with the actual requirements of peach planting specifications, the test starts sowing in the middle 11 th year of 2021. Before sowing, the test area is comprehensively sprayed with glufosinate for weeding, and deep root malignant weeds such as mugwort, nutgrass galingale rhizome, vetiver grass and glossoside are picked up by comprehensively turning over and leveling among fruit tree rows. The Chinese milk vetch, the fescue, the white clover, the ryegrass and the sesbania grass are sown in a broadcasting mode, and a thin fine soil layer is covered after sowing, and the thickness is not more than 2 cm. The management of water fertilizer, pruning, garden cleaning in winter, pest control and the like in the test area are all kept consistent.
FIG. 1 shows different flowering plants planted in a peach orchard. The left side to the right side is sequentially as follows: white clover, astragalus sinicus, ryegrass and fescue.
1.3 Investigation method
The field population density of bactrocera dorsalis was investigated in 2022, 8-9 months. The investigation was done every 5-7 days on average. And monitoring the bactrocera dorsalis by utilizing a sex-induced barrel. The repellent effect of 5 raw grasses on the bactrocera dorsalis is calculated.
Repellent effect (%) =1- (average of bactrocera dorsalis in grassland/average of bactrocera dorsalis in control land) ×100
1.4 Investigation results
As can be seen from FIG. 2, planting Astragalus sinicus, lemongrass, lespedeza sativa, lolium perennial and sesbania between peach orchards suppresses the damage of Bactrocera dorsalis to some extent. In the area where white clover, sesbania and astragalus sinicus are planted, the bactrocera dorsalis reaches the highest peak in 8 months and 25 days, wherein the quantity of bactrocera dorsalis in the area of the astragalus sinicus is the lowest, and the quantity of bactrocera dorsalis is 6.7. The area where cyrtomium praeruptorum and ryegrass were planted reached the highest peak at 9 months 1 day, and the subsequent numbers began to drop. The number of the bactrocera dorsalis reaches the highest peak (9.5) within 8 months and 25 days, which is higher than that of the bactrocera dorsalis in all grass growing areas. The number of the white clover is the most (5.1) and the number of the orange fruit flies is the least (3.3) in the astragalus mongholicus in the area of 9 months and 29 days, and the number of sesbania areas and the number of the astragalus mongholicus in the area of no significant difference.
As can be seen from Table 1, the repellent effect of Astragalus sinicus is best, and reaches 43.13%, and there is no significant difference between the repellent effects of sesbania and fescue. The repellent effect of the white clover is the worst (24.88%), and there is no obvious difference between the white clover and the ryegrass.
TABLE 1 repellent effects of different grasses on Bactrocera dorsalis
Grass seeds Repellent effect/%
Astragalus sinicus 43.13%
Sesbania (Sesbania) 32.96%
Royal jelly 32.79%
Radix Trifolium Pratentis 24.88%
Ryegrass (ryegrass) 25.20%
And (2) testing II: test of attracting effect of main metabolite of peach on Bactrocera dorsalis
Contour analysis of major metabolite of peach
(1) Experimental materials
33 Control tips and bags of Rui Pan 19, leather peach, ganalyte, gold flat and the like and peaches treated by different varieties are used as test materials, and are used for analysis after picking in the mature period, and each group of the peaches is treated for 3 times.
(2) Metabolic substance extraction method
Reagent: analysis of acetonitrile, sodium chloride, magnesium sulfate, chromatographic acetonitrile and the like
The device comprises: IKA high speed refiners, rotary evaporators (RE-2000), thermostatted shakers, centrifuge bottles, separating funnels and other laboratory instrumentation common.
Extracting: weighing the prepared peach sample in a white centrifugal bottle, adding 20mL of deionized water, adding 50mL of acetonitrile, crushing the sample by a high-speed homogenizer (20-30 seconds, depending on the state of the sample), filtering the sample by a triangular funnel with absorbent cotton plugged at the bottom to a measuring cylinder with a plug, carefully adding 6g of NaCl and 8gMgSO 4, vigorously oscillating for 1min, standing for 5-10min, and sucking 25mL of supernatant solution in a flat-bottomed flask. Concentrating, drying with nitrogen, adding 2ml of ethyl acetate to constant volume, and passing through 0.22 μm organic film into sample bottle for GC-MS/MS detection.
(3) Metabolite detection analysis method
Detection instrument: 7000C GC-MS/MS, column: DB-1
Sample introduction parameters: ion source EI temperature 230 ℃, sample inlet temperature: 250 ℃, auxiliary heater temperature: 280 ℃. The sample injection amount is 1uL, and the carrier gas rate is 1mL/min.
Heating program: 60 ℃ for 3min; raising the speed of 13 ℃/min to 200 ℃ and keeping for 1min; the rate of 25 ℃/min rises to 250 ℃ and is kept for 5min.
(4) Analysis results
As shown in FIGS. 3 and 4, 8 out of 33 peach samples had limited sensitivity detection, no volatile components were detected, and the other 25 samples detected 88 compounds such as sterols, alkanes, esters, phenols, etc.
In a single sample, a minimum of 4 volatile substances are detected, and a maximum of 37 volatile substances are detected.
As shown in FIG. 5, 17 volatile compounds were detected at a detection rate of 11.36% (10/88), stigmasterol (stigmasterol) (CAS: 83-48-7) in 16 samples, and 2-Cyclohexen-1-one (2-cyclohexen-1-one) (CAS: 7070-24-8) and Sitostero (sitosterol) (CAS: 83-47-6) in 15 samples, respectively.
2 Peach main metabolite attracting effect on Bactrocera dorsalis
The project group utilizes an attracting test to explore the attracting effect of main metabolites of peach on the bactrocera dorsalis, and provides a basis for developing an efficient bactrocera dorsalis attractant.
3 Materials and methods
(1) Insect source for test
Adult fruit fly in 2022 is captured in village before the source of Jinhua in the east country in 8 months, and male and female insects are separated after capturing.
Reagents and materials
The metabolite standard obtained by the outline analysis result of the main metabolite of the peach is not easy to purchase, so that the standard which is easy to purchase is selected for analysis. In combination with reference results, a total of 13 compounds were selected for the attraction test (Table 2). The Y-shaped olfactory instrument is self-made, a colorless transparent glass tube is adopted as a Y-shaped tube, a straight tube is 20cm long, two arms are 15cm long, the inner diameter is 1.5cm, the included angle of the two arms is 60 degrees, the tail ends of the two arms are respectively connected with a flowmeter, a 100mL flavor source bottle, a 100mL air humidifying bottle and an activated carbon adsorption bottle, the tail end of the device is an air pump, and all the devices are connected by a Teflon tube.
Table 2: reagent for attracting test
(3) Method of
And testing the behavioral response of the fruit fly adults to 13 peach metabolites by using a Y-type olfactometer, so as to screen out the peach metabolites with stronger attracting effect on the fruit fly. The gas flow rates in the two arm tubes of the Y-type olfactometer are 200mL/min, the test temperature is 25-28 ℃, the test time is 10:00-17:00, the odor sources are stigmasterol, tetracosane, phenethyl alcohol, ocimene and the like, and the concentrations of the odor sources are 10 mu L/mL.
10 Volatiles were diluted 100-fold with mineral oil as test solution, 100 μl of each volatiles solution was placed on filter paper in a flavor bottle, with mineral oil as control. The method comprises the steps of selecting adults with good crawling ability each time, inoculating the adults, carrying out random male and female sex, carrying out 1 repetition on 10 heads, carrying out 3 repetition, exchanging the directions of two arms of a Y-shaped pipe every time 5 heads are tested, cleaning an olfactometer by using 95% alcohol every time 10 heads are tested, and carrying out the next test after air drying. When the insects pass over 1/3 of an arm, the selection is considered; and if the reaction is not carried out within 5 minutes, the reaction is regarded as no reaction.
The attracting quantity of 13 peach metabolites on the fruit fly adults is counted, and the attracting rate is calculated, wherein the attracting rate is the number of insects of the selected side arm/the number of test insects multiplied by 100%.
4 Test results
The results of the attracting test show that as shown in fig. 6, 13 compounds have certain attracting effect on the bactrocera dorsalis. The attraction rate of the compound 10 to the bactrocera dorsalis is highest (72.6%), and the attraction rate of the compound 7 is lowest (24.7%). There was no significant difference between the attracting rates of compound 10 and compound 6, and there was a significant difference between the attracting rates of compound 6 and compound 4.
Conclusion 5
Among 13 compounds, the attraction rate of n-octyl phthalate and 3-isobutyl hexahydropyrrole [1,2-a ] pyrazine-1, 4-dione to bactrocera dorsalis is higher.
In addition, by using the method for preventing the bactrocera dorsalis in the invention, an exemplary base 100hm 2 is established in the Dongxiang of Jin Dongou in Jinhua city, so that a good effect of preventing the bactrocera dorsalis is achieved.
The invention is not limited to the above-described alternative embodiments, and any person who may derive other various forms of products in the light of the present invention, however, any changes in shape or structure thereof, all falling within the technical solutions defined in the scope of the claims of the present invention, fall within the scope of protection of the present invention.

Claims (10)

1. The method for preventing and controlling the bactrocera dorsalis in the peach orchard is characterized by comprising the following steps of:
S100, cleaning an orchard: intensively cleaning the insect disease fruits;
s200, fruit bagging: bagging the fruits;
S300, sowing or broadcasting the astragalus sinicus seeds in autumn and winter;
s400, setting a trap or an insect-attracting plate in 6-10 months, wherein n-octyl phthalate and/or 3-isobutyl hexahydropyrrole [1,2-a ] pyrazine-1, 4-dione are added into the trap or the insect-attracting core of the insect-attracting plate.
2. The method for controlling fruit fly in peach orchard according to claim 1, wherein in step S100, fruits on the tree are removed periodically during the fruit ripening period, and fruits falling on the ground are picked up and put into a fruit handling bag for centralized handling of the insect disease fruits.
3. The method for controlling bactrocera dorsalis in peach orchards according to claim 1, wherein in the step S200, bagging is performed 30-45 days after flowers, redundant leaves on fruit stalks or the base of clusters are removed before bagging, and meanwhile, 1 time of insecticidal and bactericidal agent is sprayed; the bag opening is tied up when the bag is sleeved, and the bottom is provided with a hole for ventilation.
4. The method for controlling bactrocera dorsalis according to claim 1, wherein in the step S300, the seed amount of milk vetch seed is 45 kg-75 kg/hm 2.
5. The method for controlling Bactrocera dorsalis in peach orchard according to claim 1, wherein in step S400, an insect attracting plate is arranged in 6-10 months, the insect attracting plate is hung on the upper back of the upper branch of peach tree, direct sunlight is avoided, 300-375 pieces/hm 2 is avoided, and after the insect body is adhered, the insect attracting plate is replaced in time and hung for 2 times continuously.
6. The method for controlling bactrocera dorsalis according to claim 1, wherein in step S400, 40-80/hm 2 traps are suspended for 6-10 months.
7. The method for controlling bactrocera dorsalis in peach orchard according to claim 1, further comprising: and a sexual attractant is added into the attracting cores of part of the traps or the attracting plates.
8. The method for controlling bactrocera dorsalis in peach orchard according to claim 1, further comprising the step of raising chickens to remove insects: raising chickens in peach orchards, and prohibiting pesticide use in orchards during the period of raising chickens.
9. The method for controlling bactrocera dorsalis in peach orchard according to claim 1, further comprising: and releasing parasitic wasps in the field.
10. The bactrocera dorsalis control attractant is characterized by comprising n-octyl phthalate and/or 3-isobutyl hexahydropyrrole [1,2-a ] pyrazine-1, 4-dione.
CN202410066451.9A 2024-01-17 2024-01-17 Method for preventing and controlling bactrocera dorsalis in peach orchard and bactrocera dorsalis preventing and controlling attractant Pending CN117918175A (en)

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