CN117883533A - Anticoccidial growth-promoting traditional Chinese medicine compound, preparation and application thereof - Google Patents
Anticoccidial growth-promoting traditional Chinese medicine compound, preparation and application thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine compound for resisting coccidiosis and promoting growth and preparation and application thereof, and relates to the technical field of veterinary medicines. The traditional Chinese medicine compound comprises beautyberry, betel nut and fructus alpiniae oxyphyllae in a mass ratio of 6-10: 4-8: 6-10; the Chinese herbal medicine has the effects of resisting insects, diminishing inflammation, stopping dysentery, stopping diarrhea, preventing and resisting insect diseases, has the capability of preventing and/or treating chicken coccidiosis, can effectively promote the growth performance of cultured animals, and has the advantages of low cost, safety, no drug resistance, environmental protection, no residue and no antibiotics. Is superior to growth-promoting antibiotics on the whole, and provides a new idea for a non-resistant, green and healthy cultivation mode. In addition, the traditional Chinese medicine compound is simple to prepare, does not need other adjuvants, and can meet the requirement of large-scale use in the breeding industry.
Description
Technical Field
The invention relates to the technical field of veterinary medicines, in particular to a traditional Chinese medicine compound for resisting coccidiosis and promoting growth and preparation and application thereof.
Background
The growth-promoting antibiotics are added into the feed for a long time in the breeding industry, so that on one hand, the opportunity of drug resistance transmission can be enlarged, and a large number of livestock and poultry and even human beings are exposed to the infection danger caused by drug-resistant strains, and on the other hand, the great hidden trouble is brought to food safety. For this reason, all growth-promoting antibiotics will be prohibited from being added to the feed, and feed enterprises will not be allowed to add veterinary prescription drugs (other than traditional Chinese medicines) to the breeding enterprises' processed feed.
Chicken coccidiosis is a type of protozoal disease caused by eimeria parasites and is a serious hazard to the chicken industry. The global poultry industry has been counted to cost up to $30 billion for economic losses due to coccidiosis. The total number of coccidia causing chicken coccidiosis is 7, wherein the eimeria tenella is most pathogenic, and parasitic in the cecum part of the chicken, the eimeria tenella can cause damage to the epithelial cells of the cecum and obstruct nutrient absorption and vitamin production necessary for organism metabolism, thereby causing blood dysentery and weight loss of the poultry, and even death.
At present, the poultry industry mainly depends on chemical medicines and anticoccidial feed additives to control coccidiosis, but can cause phenomena of drug resistance, drug residues and the like. Therefore, there is a need for more effective, environmentally friendly and safe anticoccidial drugs to control coccidiosis. The traditional Chinese medicine is gradually applied to chicken coccidiosis prevention and treatment due to the characteristics of small toxic and side effects, difficult generation of drug resistance and the like. In this regard, the traditional Chinese medicine is also widely applied to feeds or additives for preventing and treating diseases of cultured animals and promoting growth, and the cultivation mode has no resistance, is green and healthy and has wide development prospect. The traditional Chinese medicine contains various nutritional ingredients such as protein, amino acid, vitamin, polysaccharide and the like, can effectively improve the growth performance of animals, and can enhance the digestion and absorption functions; meanwhile, the composition contains a plurality of effective immunocompetent components, promotes phagocytic function of macrophages and conversion rate of lymphocytes, is favorable for inhibiting bacteria, and has obvious enhancement effect on cellular immunity and humoral immunity of organisms. Although the disease resistance effect of the traditional Chinese medicine product developed by natural plants is difficult to be better than that of antibiotics and chemical synthetic medicines, the traditional Chinese medicine greatly reduces the drug resistance risk, avoids the generation of super bacteria, and the traditional Chinese medicine also has the advantages of abundant resources, low price, small toxic and side effects, no drug resistance, difficult residue and the like. Therefore, a novel disease-resistant and growth-promoting Chinese herbal compound which is low in price, high in efficiency, safe, free of drug resistance, environment-friendly, free of residues and free of antibiotics is developed, and has important application value in animal breeding industry.
Disclosure of Invention
In order to overcome the defects and shortcomings of the feed and additives thereof in the prior art, the primary purpose of the invention is to provide the traditional Chinese medicine compound for resisting coccidiosis and promoting growth.
The invention also aims at providing a preparation method of the traditional Chinese medicine compound.
The invention also aims to provide an application of the traditional Chinese medicine compound in preparing feed or feed additives.
In the traditional Chinese medicine compound, the mass ratio of beautyberry, betel nut and fructus alpiniae oxyphyllae is 6-10: 4-8: 6 to 10. The Chinese herbal medicine compound has the effects of resisting insects, diminishing inflammation, stopping diarrhea, preventing insects and disease resistance, promoting the growth of cultured animals, and has the advantages of low cost, safety, no drug resistance, environmental protection, no residue and no antibiotics.
The aim of the invention is achieved by the following technical scheme:
The invention provides a traditional Chinese medicine compound for resisting coccidiosis and promoting growth, which comprises beautyberry, betel nut and fructus alpiniae oxyphyllae in a mass ratio of 6-10: 4-8: 6 to 10.
Wherein, the beautyberry has the effects of detumescence, stasis elimination, astringency, hemostasis, wind dispelling, dehumidification and the like, and also has a certain inhibition effect on chicken escherichia coli; fructus Alpinae Oxyphyllae has effects of warming spleen, relieving diarrhea, controlling salivation, warming kidney, reducing urination and stopping nocturnal emission. The traditional Chinese medicine considers that the fructus alpiniae oxyphyllae is warm but not hot, warm but not dry, tonic but not drastic, astringent but not diarrhea, and has the property of moderating and nourishing. Betel nut has the effects of killing parasites, removing food retention, promoting diuresis, preventing malaria, preventing chicken coccidium and stopping diarrhea. The Chinese herbal medicine compound has the effects of resisting bacteria, diminishing inflammation, stopping diarrhea, preventing insect and disease, promoting the growth of cultured animals, and has the advantages of low cost, safety, no drug resistance, environmental protection, no residue and no antibiotics.
Through experiments, as a most preferable scheme, the mass ratio of beautyberry, betel nut and fructus alpiniae oxyphyllae is 8:6:10.
Experiments on yellow-feather broilers by using the traditional Chinese medicine compound show that the performance of the broiler experimental group eating the feed added with the traditional Chinese medicine compound is superior to or equal to that of the broiler control group added with antibiotics in the aspects of average daily gain, average weight in the whole period, feed conversion rate, ratio of small intestine villus density to crypt depth, spleen index, bursa index, thymus index, antioxidant index and the like. Experiments on Ling nan quick-acting rhubarb chickens by using the traditional Chinese medicine compound show that the broiler experimental group eating the feed added with the traditional Chinese medicine compound can reduce bloody stool and intestinal lesions caused by coccidian, reduce the scores of coccidian lesions and increase the weight of chickens. The traditional Chinese medicine compound is not added with antibiotics, does not cause drug resistance, does not cause a large number of livestock and poultry and even human beings to be exposed to infection danger caused by drug-resistant strains, and does not bring hidden danger to food safety, so the traditional Chinese medicine compound is integrally superior to growth-promoting antibiotics, and can provide a new idea for a non-resistant, green and healthy cultivation mode.
Preferably, the dosage form of the traditional Chinese medicine compound is available dosage forms for animal medicines such as powder, tablets, capsules, decoction and the like.
The medicine for the cultured animals has a plurality of applicable formulations, but in the breeding industry, the medicine is often taken as powder and added into feed or feed additive for the animals to directly eat, and the main reasons are that the powder has simple preparation method, easy dispersion in the body, quick effect and suitability for taking.
When the traditional Chinese medicine compound is powder, the preparation method comprises the steps of weighing the traditional Chinese medicine raw materials in parts by weight, crushing, drying and uniformly mixing.
The powder has simple preparation process, low cost of the required raw materials, no hormone or antibiotic, accords with the trend of industry future resistance reduction, replacement resistance and environmental protection, and has great practical significance for the green and healthy cultivation mode.
Preferably, the traditional Chinese medicine raw materials are crushed and then are sieved by a sieve of 80-150 meshes.
Preferably, the drying is at 60 ℃.
The invention also claims the application of the traditional Chinese medicine compound in preparing feed or feed additive.
Preferably, the addition amount of the traditional Chinese medicine compound is 0.1-0.3% of the weight of the feed.
Most preferably, the test animals gain most weight when 0.1% is added to the feed, the feed conversion rate is also optimal, and the anticoccidial effect is optimal when 0.3% is added to the feed.
Specifically, the traditional Chinese medicine compound can promote the growth of broiler chickens, promote digestion and absorption of broiler chickens, improve the immunity of broiler chickens, prevent and/or treat coccidiosis of chickens and the like.
The promotion of digestion and absorption of the broiler chicken is to increase the height of the nap of the broiler chicken and/or increase the ratio of the height of the nap of the small intestine of the broiler chicken to the depth of the crypt of the small intestine.
The improving the immunity of the broiler chickens is to improve the cell immunity function of the broiler chickens and/or improve the humoral immunity function of the broiler chickens.
Compared with the prior art, the invention has the following advantages and effects:
The Chinese herbal medicine compound has the effects of resisting insects, diminishing inflammation, stopping diarrhea, preventing insects and disease resistance, has the capability of preventing and/or treating chicken coccidiosis, can effectively promote the growth performance of cultured animals, and is low in cost, safe, free of drug resistance, environment-friendly, free of residues and free of antibiotics. Is superior to growth-promoting antibiotics on the whole, and provides a new idea for a non-resistant, green and healthy cultivation mode. In addition, the traditional Chinese medicine compound is simple to prepare, does not need other adjuvants, and can meet the requirement of large-scale use in the breeding industry.
Drawings
FIG. 1 is a graph of intestinal mucosa tissue of jejunum of each group of broilers in example 1; wherein a: blank group; b: antibiotic group (aureomycin 75 mg/kg); c: chinese herbal compound 1 (1 g/kg); d: chinese herbal compound 2 (3 g/kg).
FIG. 2 is the bloody stool of each group of broilers in example 2; wherein A: a traditional Chinese medicine compound group D; b: a traditional Chinese medicine compound group G; c: a model group; d: blank control group; e: sensitive pharmaceutical group.
FIG. 3 shows the results of cecal lesions of groups of broilers from example 2; wherein A: a traditional Chinese medicine compound group D; b: a traditional Chinese medicine compound group G; c: a sensitive drug group; d: a model group; e: blank control group.
FIG. 4 is the bloody stool of each group of broilers in example 3; wherein A: a traditional Chinese medicine compound group G; b: bare: benefit = 8:6 group; c: bare: set of champagne=8:10; d: the beneficial effects are that: champagne = 6:10 group; e: fructus Alpinae Oxyphyllae group; f: a beautyberry group; g: betel nut group; h: a model group; i: a sensitive drug group; j: blank control group.
FIG. 5 shows the results of cecal lesions of groups of broilers from example 3; wherein A: blank control group; b: a traditional Chinese medicine compound group G; c: a model group; d: bare: benefit = 8:6 group; e: bare: set of champagne=8:10; f: the beneficial effects are that: champagne = 6:10 group; g: a beautyberry group; h: fructus Alpinae Oxyphyllae group; i: betel nut group; j: sensitive pharmaceutical group.
Detailed Description
The present invention will be described in further detail with reference to examples and drawings, but embodiments of the present invention are not limited thereto. Unless otherwise indicated, the materials used in the examples of the present invention were conventionally purchased.
Example 1
1. Experimental materials
The traditional Chinese medicine compound group: the mass ratio is 8:6:10, respectively weighing raw materials of beautyberry, betel nut and fructus alpiniae oxyphyllae, crushing, drying in a hot air drying oven at 60 ℃, sieving with a 80-mesh sieve, uniformly mixing to obtain a traditional Chinese medicine compound, and respectively mixing the traditional Chinese medicine compound into two groups with feed uniformly, wherein the addition amount of the traditional Chinese medicine compound in the feed is 1g/kg (traditional Chinese medicine compound/feed) and 3g/kg (traditional Chinese medicine compound/feed).
Antibiotic group (aureomycin 75 mg/kg): the aureomycin and the feed are uniformly mixed, so that the mass ratio of the aureomycin to the feed is 75mg/kg (aureomycin/feed).
2. Experimental method
100 Yellow feather broilers of 1 day old are selected and divided into four groups, namely a blank group (feed only), an antibiotic group (aureomycin 75 mg/kg), a traditional Chinese medicine compound group 1 (1 g/kg) and a traditional Chinese medicine compound group 2 (3 g/kg), weighing is carried out, the average empty weight of each group is calculated, and the chickens are fed for 28 days, are free to drink water during the period, so that good ventilation and proper temperature are ensured. After 28 days of feeding, recording feed intake, weighing on an empty stomach, and calculating Average Daily Gain (ADG), average weight in whole period and feed-weight ratio (F/G) of each group of experimental broilers; the method comprises the steps of performing neck breaking and sacrifice after jugular vein blood sampling, rapidly taking the middle section of a jejunum, adding 20mL of 4% paraformaldehyde phosphate buffer solution, fixing for 48 hours (keeping pH=7.4), embedding with conventional paraffin, preparing 10 continuous transverse sections (thickness of 5 μm), and measuring the villus height and the crypt depth of the small intestine and calculating the ratio after staining with hematoxylin-eosin (H.E.); spleen, thymus, bursa were collected, surrounding fat was removed and the water was blotted with filter paper, weighed and recorded, and an immune organ index [ immune organ index (g/kg) =immune organ fresh weight (g)/live weight (kg) ] was calculated. The collected blood is kept still, and then centrifuged for 10min at 3 000r/min, the separated serum is preserved at-80 ℃, to be tested, and the content of SOD, T-AOC and MDA in the serum is measured according to the operation steps of the instruction book of the kit.
3. Experimental results
The experimental results are shown in 1-6.
Table 1 average daily gain results for broiler chickens (x±s.e, n=25)
Note that: each marked letter is different indicating a significant difference (P < 0.05).
As can be seen from Table 1, compared with the blank group, the antibiotic group (aureomycin 75 mg/kg), the traditional Chinese medicine compound group 1 (1 g/kg) and the traditional Chinese medicine compound group 2 (3 g/kg) have certain effects on daily gain of the broiler chickens, wherein the daily gain effect of the traditional Chinese medicine compound group 1 (1 g/kg) on the broiler chickens is more obvious, and the average daily gain of the broiler chickens can be improved.
Table 2 average weight results of broiler chickens throughout the period
Note that: each marked letter is different indicating a significant difference (P < 0.05).
As can be seen from Table 2, the antibiotic group (aureomycin 75 mg/kg), the Chinese herbal compound group 1 (1 g/kg) and the Chinese herbal compound group 2 (3 g/kg) have certain effects on the average weight of the whole period of the broiler chickens compared with the blank group, wherein the weight gain effect of the Chinese herbal compound group 1 (1 g/kg) on the whole period growth of the broiler chickens is more obvious, and the difference is obvious (P < 0.05).
Table 3 results of feed to weight ratio of broiler chickens
Note that: each marked letter is different indicating a significant difference (P < 0.05).
As shown in Table 3, the feed conversion rate of the compound Chinese medicine group 1 (1 g/kg) is obviously reduced compared with the blank group (P < 0.05).
Table 4 index value of small intestine digestion and absorption function of broiler chickens
Note that: each marked letter is different indicating a significant difference (P < 0.05).
The quality of intestinal mucosa structure can directly influence the absorption and utilization of nutrient substances by animals, and the ratio of the villus height to the crypt depth of the small intestine is an important index for measuring the digestion and absorption functions of the small intestine. As can be seen from the data in fig. 1 and the analysis table 4, compared with the antibiotic group (aureomycin 75 mg/kg), the depth of the small intestine crypt of the broiler chicken in the compound group 1 (1 g/kg) is reduced, and the villus height and the V/C value (small intestine villus height/small intestine crypt depth) are both obviously improved (P < 0.05), namely, the compound group can improve the intestinal mucosa structure, increase the density of small intestine villus, and increase the small intestine villus height, so that the small intestine absorption area is increased, and the compound group 1 has a promoting effect on maintaining healthy development of the intestinal tract and the functions of intestinal mucosa epithelial cells, and is beneficial to absorption and utilization of nutrient substances, thereby promoting growth of animals.
Comprehensive tables 1 to 4 show that after the feed added with the traditional Chinese medicine compound is eaten, the weight growth, feed conversion rate and digestion and absorption functions of the broiler chickens are all obviously improved, wherein when the adding amount of the traditional Chinese medicine compound is 0.1% of the using amount of the feed, the effect is optimal, and the growth of the broiler chickens can be obviously promoted.
TABLE 5 immune organ index results of broiler chickens
Spleen, thymus and bursa are organs of the poultry organism which exert immune effects, and the immune indexes of the organs can be detected to preliminarily measure the immune state of the organism and the development condition of the immune system. As can be seen from Table 5, the spleen index and bursa of Fabricius index of the compound Chinese medicine group 1 (1 g/kg) are improved to a certain extent, which belongs to the increase in the normal range, reflects the enhancement of the immune function of animals to a certain extent, and is beneficial to promoting the organism to generate immune cells and improving the vaccine antibody level. The results show that the feed added with the traditional Chinese medicine compound of the invention has the effect of obviously improving the cell immunity and humoral immunity of the broiler chickens. The spleen index of the aureomycin group tended to decrease, suggesting a decrease in immunocompetence.
TABLE 6 serum antioxidant index detection
Note that: each marked letter is different indicating a significant difference (P < 0.05).
The antioxidant capacity of an animal body can reflect to some extent the immune function and anti-infective capacity of the body. SOD can catalyze superoxide anion free radical O 2- in the organism to be differentiated into H 2O2 and O 2, remove oxygen free radical in the organism, reduce oxidation damage in the organism, and SOD is an index reflecting the capability of the organism to remove free radical. T-AOC can reflect to some extent the total ability of the body to scavenge ROS/NOS (oxidized free radicals). MDA is an important marker product of lipid peroxidation, suggesting lipid peroxidation strength.
Compared with the blank group, the total antioxidant capacity (T-AOC) of the traditional Chinese medicine compound group is improved. The superoxide dismutase (SOD) activity of the traditional Chinese medicine compound group 2 (3 g/kg) is obviously improved (P is less than 0.05). The content of Malondialdehyde (MDA) in the traditional Chinese medicine compound group 1 (1 g/kg) is obviously reduced (P is less than 0.05) compared with the content of a blank group and an antibiotic group, which proves that the traditional Chinese medicine compound group 1 (1 g/kg) can obviously improve the oxidation resistance of broilers.
In conclusion, the performance of the broiler experimental group eating the feed added with the traditional Chinese medicine compound is superior or equivalent to that of the broiler control group eating the feed added with antibiotics in the aspects of average daily gain, average weight in whole period, feed conversion rate, ratio of small intestine villus density to crypt depth, spleen index, antioxidation index and the like, and the traditional Chinese medicine compound does not add antibiotics, does not cause drug resistance, does not cause a great deal of livestock and poultry even human to be exposed to infection danger caused by drug-resistant strains, and does not bring hidden trouble to food safety, so the traditional Chinese medicine compound is superior to growth-promoting antibiotics as a whole, and can provide a new idea for a non-resistant, green and healthy cultivation mode.
Example 2
1. Experimental materials
The traditional Chinese medicine compound group: 80g of beautyberry, 60g of betel nut and 100g of fructus alpiniae oxyphyllae according to the mass ratio of 8:6:10, weighing the raw materials, crushing, drying in a hot air drying oven at 60 ℃, sieving with a 80-mesh sieve, and uniformly mixing to obtain the traditional Chinese medicine compound.
Sensitive pharmaceutical group (mix 1g/1 kg): the three-word coccidium powder (namely, 30% sulfachloropyrazine sodium soluble powder, 100 g/bag, purchased from Anmu fish biotechnology Co., ltd.) was uniformly mixed with the feed so that the mass ratio thereof was 1g/kg (drug/feed).
2. Experimental method
100 Healthy, substantially uniform weight, ling nan quick-acting rhubarb chickens were selected and randomly divided into 5 groups (20 per group) and dosing was started at 12 days of age. The test groups are shown in Table 7, the blank control group is fed with a non-resistant basic ration, 1G/kg (and 3G/kg) of the traditional Chinese medicine compound group D and the traditional Chinese medicine compound group G are respectively added into the non-resistant basic ration, the traditional Chinese medicine compound group D and the traditional Chinese medicine compound group G are pre-fed for one week (namely 5-11 days old), after weighing one by one, lean or oversized chickens are removed, healthy chickens with individual weight difference within 10G are selected, and each drug test group and each non-dosing insect attack test group are respectively inoculated with 1.0X10 4 Eimeria tenella GD sporulation oocysts through mouth at 14 days old. The test period was 9 days. In the test period, the chickens only eat and drink water freely, the constant illumination is carried out for 16 hours every day, and the fan is ventilated. The health condition of the chicken flock is monitored, the number of dead panning chickens and the reasons thereof are recorded, and regular disinfection is carried out in the house. Wherein, the Eimeria tenella GD strain (i.e., eimeria tenella Guangdong strain) sporulates oocysts, which are maintained by the national institute of animal health, national academy of agricultural sciences, guangdong.
Table 7 test design grouping
The test index was determined as follows:
(1) Score for bloody stool
Fecal scoring was performed by reference to the Sonchun (1997) method. Fifth day after attack, observing blood-stool proportion, photographing and recording, and scoring blood-stool: for Eimeria tenella infection, a score of 0 indicates 100% fecal bloodless, +1 indicates 25% fecal bloodless, +2 indicates 50% fecal bloodless, +3 indicates 75% fecal bloodless, and +4 indicates 100% fecal bloodless for a given 12-24h time frame.
(2) Relative weight gain rate
The chicken weights were weighed at the beginning and end of the test, and the average weight gain and the relative weight gain rate were calculated. Relative weight gain = (test group weight gain/no dosing no challenge test group weight gain) x 100%.
(3) Survival rate
The number of dead chickens in each group is recorded, the death factor is determined by the section inspection, and the survival rate is calculated. Survival = (number of surviving chickens at the end of the trial/number of experimental group chickens) ×100%.
(4) Lesion value
Slaughtering the chickens on the 7 th day after infection, and referring to a lesion scoring method designed by Johnson and Reid (1970), scoring intestinal lesions of each chicken, and converting the lesion scores into lesion values;
lesion scoring: (when the cecal lesions on both sides are inconsistent, the serious side is the reference):
0 minutes, no macroscopic lesions;
1 minute, the cecum wall has a small amount of scattered stasis points, the intestinal wall is not thickened, and the content is normal;
2 minutes, the number of lesions is large, the cecum content is obviously bloody, the cecum wall is slightly thickened, and the content is normal;
3 minutes, the cecum has a large amount of blood or cecum cores (blood clots or gray cheese-like banana-shaped blocks), the wall of the cecum is thickened obviously, and the fecal content in the cecum is low;
4 minutes, the cecum is enlarged due to the filling of a large amount of blood or intestinal cores, the intestinal cores contain or do not contain manure, and the death of the chicken due to coccidiosis is only recorded as 4 minutes.
Lesion values (0-40) =average lesion score for each test group (0-4) ×10.
Lesion score reduction Rate (RLS) = (mean lesion score for non-infected control group-mean lesion score for infected control group)/mean lesion score for non-infected control group 100%.
Lesion score reduction Rate (RLS) criteria: the drug control group RLS < = 30% is judged to have drug resistance, 31-49% of the drug resistance is partly, and > = 50 is sensitive.
(5) Oocyst value and relative oocyst yield (ROP)
Fecal oocyst counts were performed using the macmaster count method, and the number of fecal Oocysts Per Group (OPG) was determined and converted to oocyst values according to table 8.
Relative oocyst yield (ROP) = (average oocyst yield in infected/average oocyst yield in infected non-administered) ×100%. ROP is more than or equal to 15 percent, and has drug resistance; ROP <15%, no drug resistance.
TABLE 8 conversion of oocyst count to oocyst value
(6) Anticoccidial index (ACI)
According to the formula of Merck (Merck) in the united states, namely:
aci= (relative weight gain rate + survival rate) ×100- (lesion value + oocyst value).
Drug efficacy criterion: ACI >180 is a highly effective anticoccidial; 160< aci <180 genus moderate anticoccidial; 120< aci <160 genus of low potency anticoccidial; ACI <120 is not effective against coccidium.
Test results
(1) Clinical symptoms
The reaction of reduced feeding, poor spirit and the like gradually appears after the sporulated oocysts are infected by the test chickens. Part of medicine groups and model groups have different degrees of blood and feces discharge after infection, the water intake is reduced, the disease is more serious on the 5 th day and the 6 th day, the cecum disease is killed and detected on the 7 th day of attack, the bleeding or blood clot with different degrees is caused, and other organs do not observe the disease; the blank control groups have no blood manure, and the drinking water is normal.
(2) Blood stool score and cecum lesion result
The observation result of blood and stool in the fifth day after attack of insects is available, and the blood and stool condition of the traditional Chinese medicine compound group D, G shows +1 score; model group blood stool condition presents +3 points; the blood stool condition of the sensitive medicine group and the blank control group is 0 point. The blood stool results are shown in fig. 2, and the cecum lesions results are shown in fig. 3.
(3) Results of drug sensitivity test
And (3) result prompting: in the test medicine, ACI indexes of the traditional Chinese medicine compound group D, G show low-efficiency anticoccidial effect under mixed administration; wherein, the traditional Chinese medicine compound group G has the lowest oocyst yield (ROP) of Eimeria tenella GD strain, the highest lesion score reduction Rate (RLS), higher relative weight gain rate and the best anticoccidial effect. All index effects of the sensitive medicine group are obviously improved, and the anticoccidial effect with high equivalent efficacy is achieved. The results are detailed in Table 9.
TABLE 9 results of drug sensitivity test
Example 3
In order to verify whether the combined prescription of the three traditional Chinese medicines is better than the effect of two medicines or a single medicine, a further experiment is carried out. This time, the preferred 3g/kg dose was used as a unified dose for adding the traditional Chinese medicine, and the other steps were the same as in example 2, and the detailed group is shown in Table 10.
TABLE 10 drug sensitivity test 2 grouping
Test results
(1) Clinical symptoms
The reaction of reduced feeding, poor spirit and the like gradually appears after the sporulated oocysts are infected by the test chickens. Part of the medicine groups and the model groups have different degrees of blood and feces discharge after infection, the water intake is reduced, the disease is more serious on the 5 th day and the 6 th day, the cecum disease is killed and detected on the 7 th day of attack, the bleeding or blood clot with different degrees is caused, and the disease is not observed in other organs; the blank control groups have no blood manure, and the drinking water is normal.
(2) Blood stool score and cecum lesion result
The observation result of blood and stool in the fifth day after attack of insects is available, and the blood and stool condition of the traditional Chinese medicine group shows +1 score; model group blood stool condition presents +3 points; the blood stool condition of the sensitive medicine group and the blank control group is 0 point. The blood stool results are shown in FIG. 4, and the cecal lesions results are shown in FIG. 5.
(3) Results of drug sensitivity test
And (3) result prompting: in the test medicine, the ACI index of the traditional Chinese medicine compound group G shows a low-efficiency anticoccidial effect under the administration of 3G/kg; wherein, the traditional Chinese medicine compound group G has the lowest oocyst yield (ROP) of Eimeria tenella GD strain, the highest lesion score reduction Rate (RLS), higher relative weight gain rate and the best anticoccidial effect. All index effects of the sensitive medicine group are obviously improved, and the anticoccidial effect with high equivalent efficacy is achieved. The results are detailed in Table 11.
TABLE 11 results of drug sensitivity test 2
The above examples are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above examples, and any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principle of the present invention should be made in the equivalent manner, and the embodiments are included in the protection scope of the present invention.
Claims (10)
1. A traditional Chinese medicine compound for resisting coccidiosis and promoting growth is characterized in that: the traditional Chinese medicine compound comprises beautyberry, betel nut and fructus alpiniae oxyphyllae, and the mass ratio is 6-10: 4-8: 6 to 10.
2. The traditional Chinese medicine compound according to claim 1, wherein:
The mass ratio of the beautyberry to the betel nut to the fructus alpiniae oxyphyllae is 8:6:10.
3. The traditional Chinese medicine compound according to claim 1 or 2, characterized in that:
The formulation of the traditional Chinese medicine compound is powder, tablet, capsule or decoction.
4. The method for preparing the traditional Chinese medicine compound according to claim 1 or 2, which is characterized in that: the traditional Chinese medicine compound preparation is powder, and is prepared by weighing the traditional Chinese medicine raw materials in parts by weight, crushing, drying and uniformly mixing.
5. The method of manufacturing according to claim 4, wherein: the traditional Chinese medicine raw materials are crushed and then are sieved by a sieve of 80-150 meshes.
6. The method of claim 4 or 5, wherein: the drying is drying at 60 ℃.
7. Use of a herbal compound as claimed in any one of claims 1 to 3 for the preparation of a feed or feed additive.
8. The use according to claim 7, characterized in that:
The addition amount of the traditional Chinese medicine compound is 0.1-0.3% of the weight of the feed.
9. Use according to claim 7 or 8, characterized in that:
The application of the traditional Chinese medicine compound in preparing feed or feed additive for at least one of promoting the growth of broiler chickens, promoting digestion and absorption of broiler chickens, improving the immunity of broiler chickens, preventing chicken coccidiosis and treating chicken coccidiosis.
10. The use according to claim 9, characterized in that:
the promotion of digestion and absorption of the broiler chicken is to increase the height of the nap of the broiler chicken and/or increase the ratio of the height of the nap of the small intestine of the broiler chicken to the depth of the recess of the small intestine;
The improving the immunity of the broiler chickens is to improve the cell immunity function of the broiler chickens and/or improve the humoral immunity function of the broiler chickens.
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