CN117866743A - Lactobacillus johnsonii strain culture equipment - Google Patents

Abstract

The invention discloses lactobacillus johnsonii strain culture equipment, and particularly relates to the technical field of strain culture, which comprises an incubator, wherein a rack is arranged in the incubator, a plurality of culture components for culturing lactobacillus johnsonii strains are placed on the rack, the rack comprises a bracket, a plurality of movable plates distributed up and down are arranged in the bracket, the centers of two sides of the movable plates are rotationally connected with the bracket through a rotating shaft, the top end of each movable plate is fixedly provided with a placement frame, two placement cavities are arranged in the placement frame, a plurality of culture components are uniformly placed in the placement frames of the placement cavities, connecting rods are arranged at four corners between two adjacent movable plates, and an electric push rod is fixed at the bottom end inside the incubator. According to the invention, the plurality of movable plates are driven to shake by the reciprocating expansion of the electric push rod, so that the culture assembly on the movable plates shakes, and then the lactobacillus johnsonii strain, oxygen and a culture medium in the culture assembly are uniformly mixed, thereby being beneficial to the growth and metabolism of the lactobacillus johnsonii strain and improving the propagation speed of the strain.

Description

Lactobacillus johnsonii strain culture equipment

Technical Field

The invention relates to the technical field of strain culture, in particular to lactobacillus johnsonii strain culture equipment.

Background

Lactobacillus johnsonii is a microorganism of the genus lactobacillus and can be used for brewing wine and also for agriculture. When the method is applied to brewing wine, the Lactobacillus johnsonii can start and finish MLF under the low-temperature condition, and can improve the fragrance quality of the wine. When the lactobacillus johnsonii is applied to agriculture, the lactobacillus johnsonii can realize: 1. regulating the micro-ecological environment for plant growth, and providing optimal environment for healthy growth of crops, wherein the micro-ecological environment for plant growth is facultative anaerobic metabolism and does not compete with crops for oxygen; 2. promoting the root system development of crops and expanding the nutrition absorption range; 3. the disease resistance of crops is improved; 4. soil is raised, improved, formation of soil aggregate structures is promoted, and soil is loosened; 5. the stress resistance, drought resistance, waterlogging resistance and freezing resistance of crops are improved, and the quality of agricultural products of the crops is obviously improved.

The lactobacillus johnsonii strain is cultivated by means of a manually prepared culture medium and artificially created culture conditions, and can grow and reproduce rapidly, for example, a strain cultivation machine with the prior art publication No. CN115197811A can be used for cultivation, a strain cultivation disk is placed on a placement frame, and then the placement frame is pushed into the interior of a cultivation tank for strain cultivation; and can cultivate the material at the culture process, the material of passing through the external connection pipe of the front and the back of culture tank that sets up can get into the inside of split plate through the hose at first, then set up the inside that the splitter box lets in the ventilation groove through split plate opposite side, then transmit for the water conservancy diversion piece through the ventilation groove, get into the inside in upper chamber and lower chamber respectively through the water conservancy diversion piece material, the material of getting into the lower intracavity portion can be surging through the gas outlet, and the material of getting into the upper chamber inside can be surging through the outlet duct, then directly act on the inside bacterial of culture dish.

When the microorganism is cultured in the liquid culture medium, the strain and the liquid culture medium are required to be stirred to be uniformly mixed, meanwhile, sterile air can be continuously introduced into the container, so that the growth and metabolism of the strain are facilitated, the microorganism can be rapidly propagated after being fully contacted with oxygen and the culture medium, but in the prior art, the material cannot be uniformly mixed with the strain after being introduced into the culture disc, so that the strain propagation speed is low, and the culture effect is poor.

Disclosure of Invention

In order to overcome the defects in the prior art, the invention provides the lactobacillus johnsonii strain culture equipment, which drives a plurality of movable plates to shake by reciprocating and stretching the electric push rod, so that a culture assembly on the movable plates shakes, and further, the lactobacillus johnsonii strain, oxygen and a culture medium in the culture assembly are uniformly mixed, thereby being beneficial to the growth and metabolism of the lactobacillus johnsonii strain and improving the propagation speed of the strain.

In order to achieve the above purpose, the present invention provides the following technical solutions: the utility model provides a lactobacillus johnsonii strain culture apparatus, includes the incubator, the inside back wall of incubator is fixed with the heater, the inside rack that is equipped with of incubator has placed a plurality of culturing components that are used for cultivateing lactobacillus johnsonii strain on the rack, and places to erect in the heater front side for provide the required temperature of cultivateing.

The placing rack comprises a support, a plurality of movable plates which are distributed up and down are arranged in the support, the centers of two sides of each movable plate are rotationally connected with the support through a rotating shaft, a placing frame is fixed at the top end of each movable plate, two placing cavities are arranged in the placing frame, a plurality of culture components are uniformly placed in the placing frames of the placing cavities, connecting rods are arranged at four corners between every two adjacent movable plates, and two ends of each connecting rod are rotationally connected with the two movable plates respectively;

the bottom end of the inside of the incubator is fixedly provided with an electric push rod, the electric push rod is arranged at the rear side of the bottom of the bottommost movable plate, the top end of a piston rod of the electric push rod is in contact with the bottom of the movable plate, and a plurality of groups of movable plates are driven to rotate through the expansion and contraction of the electric push rod, so that a culture assembly on the movable plate shakes, and lactobacillus johnsonii strains, oxygen and a culture medium in the culture assembly are uniformly mixed;

and the rear side of the incubator is provided with a ventilation assembly.

Preferably, the culture assembly comprises a culture dish and a dish cover with a sealing cover arranged at the top of the culture dish, wherein a connecting pipe is fixed at the top end of the dish cover and is communicated with the interior of the culture dish, and a filter screen for blocking the lactobacillus johnsonii strain from entering is fixed at the bottom of the connecting pipe.

Preferably, each of the front side wall and the rear side wall of the placing cavity is provided with a fixing component for fixing the culture component, each group of fixing components comprises a hollow plate and clamping plates arranged in the hollow plate, one side of each clamping plate extends out of the hollow plate, the other side of each clamping plate is connected with the inner wall of the hollow plate through a spring, the top of one side of each clamping plate extending out of the hollow plate is processed into an inclined surface, the hollow plate is fixed on the inner wall of the placing cavity, and two clamping plates in one placing cavity are arranged on the front side and the rear side of the culture plate and used for clamping and fixing the culture plate.

Preferably, each movable plate bottom four corners all is equipped with the rubber pad, and rubber pad and movable plate contact, and a plurality of rubber pads all are fixed in on the support, be connected with the torsional spring between movable plate and the support, and the torsional spring cover is located in the pivot between movable plate and the support.

Preferably, the four corners of the bottom end of the support are respectively fixed with a movable wheel, two wheel grooves are fixed at the bottom end of the inside of the incubator, and the two movable wheels on the same side are clamped in one wheel groove for limiting the movable wheels, so that the stability of the support is improved.

Preferably, the connecting pipes of the plurality of groups of culture components on each movable plate are connected through connecting pipes, a conveying pipe is fixed on one side of the culture box, one end of the conveying pipe extends into the culture box and is connected with the plurality of connecting pipes, and an air conveying pipe and a culture medium conveying pipe are fixed on one end of the conveying pipe, which is positioned outside the culture box, and are used for conveying oxygen and culture medium into the culture components respectively.

Preferably, the ventilation assembly comprises a box body fixed at the rear side of the incubator, a ventilation pipe with a valve is fixed at one side of the incubator, air is conveniently introduced into the box body, and a plurality of sterilizing lamps for sterilizing the air in the box body are fixed at the top end inside the box body.

Preferably, the front wall inside the box is fixed with communicating pipe, communicating pipe and incubator inside intercommunication, and the communicating pipe is located the heater bottom, be fixed with the motorised valve on the communicating pipe, the aseptic air in the box of being convenient for gets into in the incubator.

Preferably, the bottom of one side of the incubator is fixedly provided with a vacuum pump, and an air inlet of the vacuum pump is fixedly provided with an air exhaust pipe, and the air exhaust pipe penetrates one side of the incubator and is communicated with the inside of the incubator, so that air in the incubator is conveniently exhausted.

Preferably, a controller for controlling the heater, the electric push rod, the vacuum pump and the electric valve is fixed at the top of one side of the incubator.

The invention has the technical effects and advantages that:

1. placing lactobacillus johnsonii strains in a culture tray of a culture assembly, placing the culture tray in a placing frame, fixing the culture tray by using a fixing assembly, and connecting a connecting pipe of the culture assembly with a conveying pipe by a connecting pipe; in the culture process, sterile air is injected into the culture tray through the air conveying pipe, the connecting pipe and the connecting pipe by utilizing the air pump, a culture medium is injected into the culture tray through the culture medium conveying pipe, the connecting pipe and the connecting pipe by utilizing the conveying pump, and simultaneously, the plurality of movable plates are driven to shake by reciprocating telescopic motion of the electric push rod, so that a culture assembly on the movable plates shakes, and further, lactobacillus johnsonii strains, oxygen and the culture medium in the culture assembly are uniformly mixed, thereby being beneficial to growth and metabolism of the lactobacillus johnsonii strains and improving the propagation speed of the strains;

2. injecting air into the ventilation assembly through the ventilation pipe, and sterilizing the air in the box body through the sterilizing lamp, so that sterile air is stored in the box body; after pushing the rack with the cultivation subassembly into the incubator, take out the incubator internal air through the vacuum pump and make incubator internal negative pressure, then open the motorised valve on the communicating pipe, aseptic air in the box gets into the incubator through communicating pipe to with incubator internal air replacement, make the cultivation subassembly be in aseptic environment and cultivate, reduce the probability that the lactobacillus johnsonii bacterial was polluted in the cultivation subassembly, improve the cultivation effect.

Drawings

FIG. 1 is a front view of the present invention;

FIG. 2 is a rear view of the present invention;

FIG. 3 is a front view of the interior of the incubator of the present invention;

FIG. 4 is a top view of the interior of the incubator of the present invention;

FIG. 5 is an interior side view of the incubator of the present invention;

FIG. 6 is a block diagram of a rack and culture assembly of the present invention;

FIG. 7 is a block diagram of a rack and culture assembly of the present invention;

FIG. 8 is a bottom view of the bracket and flap of the present invention;

FIG. 9 is a block diagram of a movable plate, a placement frame, a stationary assembly and a culturing assembly according to the present invention;

FIG. 10 is a view showing a construction of a culture assembly according to the present invention;

FIG. 11 is a side cross-sectional view of the securing assembly of the present invention;

FIG. 12 is a horizontal schematic view of a fly leaf according to the invention;

fig. 13 is a rotating schematic view of the movable plate according to the present invention.

The reference numerals are: 1 incubator and 2 heater;

3 a placing frame, a 31 support, a 32 movable plate, a 33 placing frame, a 34 fixing component, a 35 connecting rod, a 36 moving wheel and a 37 rubber pad;

341 hollow plate, 342 clamping plate, 343 spring;

4, a culture assembly, a 41 culture disc, a 42 disc cover and a 43 connecting tube;

5, an electric push rod;

6, a ventilation assembly, a 61 box body, a 62 sterilizing lamp, a 63 communicating pipe and a 64 ventilating pipe;

7 wheel grooves, 8 conveying pipes, 9 connecting pipes, 10 air conveying pipes, 11 culture medium conveying pipes, 12 vacuum pumps and 13 exhaust pipes.

Detailed Description

The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

As shown in fig. 1 to 5, the invention provides lactobacillus johnsonii strain culture equipment, which comprises an incubator 1, wherein a heater 2 is fixed on the rear wall inside the incubator 1, a placing frame 3 is arranged inside the incubator 1, a plurality of culture assemblies 4 for culturing lactobacillus johnsonii strains are placed on the placing frame 3, and the placing frame 3 is arranged on the front side of the heater 2; the inside of the incubator 1 is heated by the heater 2, and the temperature of the heater 2 is controlled by a controller at the top of one side of the incubator 1 to provide a desired temperature for the culture of the lactobacillus johnsonii strain.

As shown in fig. 6-12, the culture assembly 4 comprises a culture dish 41 and a dish cover 42 sealing the top of the culture dish 41, lactobacillus johnsonii strains are placed in the culture dish 41 for culture, a connecting pipe 43 is fixed at the top end of the dish cover 42, the connecting pipe 43 is communicated with the interior of the culture dish 41, and a filter screen for blocking the lactobacillus johnsonii strains from entering is fixed at the bottom of the connecting pipe 43;

the rack 3 comprises a bracket 31, movable wheels 36 are fixed at four corners of the bottom end of the bracket 31, two wheel grooves 7 are fixed at the bottom end inside the incubator 1, and the two movable wheels 36 on the same side are clamped in one wheel groove 7 and used for limiting the movable wheels 36, so that the stability of the bracket 31 is improved;

a plurality of movable plates 32 which are distributed up and down are arranged in the support 31, the centers of two sides of each movable plate 32 are rotationally connected with the support 31 through a rotating shaft, a placement frame 33 is fixed at the top end of each movable plate 32, two placement cavities are arranged in the placement frame 33, a plurality of culture components 4 are uniformly placed in the placement frames 33 of the placement cavities, connecting rods 35 are arranged at four corners between every two adjacent movable plates 32, and two ends of each connecting rod 35 are respectively rotationally connected with the two movable plates 32;

the bottom end inside the incubator 1 is fixedly provided with an electric push rod 5, the electric push rod 5 is arranged at the rear side of the bottom of the bottommost movable plate 32, the top end of a piston rod of the electric push rod 5 is in contact with the bottom of the movable plate 32, and a plurality of groups of movable plates 32 are driven to rotate through the expansion and contraction of the electric push rod 5, so that a culture assembly 4 on the movable plate 32 shakes, and further lactobacillus johnsonii strains, oxygen and a culture medium in the culture assembly 4 are uniformly mixed;

rubber pads 37 are arranged at four corners of the bottom of each movable plate 32, the rubber pads 37 are in contact with the movable plates 32, a plurality of rubber pads 37 are fixed on the support 31, torsion springs are connected between the movable plates 32 and the support 31, the torsion springs are sleeved on rotating shafts between the movable plates 32 and the support 31, the rubber pads 37 can be used for supporting the movable plates 32, and the rubber pads 37 can be bent at will due to elasticity, so that the rotation of the movable plates 32 cannot be hindered;

the front side wall and the rear side wall of each placing cavity are respectively provided with a fixing component 34 for fixing the culture component 4, each group of fixing components 34 comprises a hollow plate 341 and a clamping plate 342 arranged in the hollow plate 341, one side of each clamping plate 342 extends out of the hollow plate 341, the other side of each clamping plate 342 is connected with the inner wall of the hollow plate 341 through a spring 343, the top of one side of each clamping plate 342 extending out of the hollow plate 341 is processed into an inclined surface, the hollow plate 341 is fixed on the inner wall of the placing cavity, and two clamping plates 342 in one placing cavity are arranged on the front side and the rear side of the culture plate 41 and are used for clamping and fixing the culture plate 41;

and the connecting pipes 43 of the multiple groups of culture components 4 on each movable plate 32 are connected through the connecting pipes 9, a conveying pipe 8 is fixed on one side of the culture box 1, one end of the conveying pipe 8 extends into the culture box 1 and is connected with the multiple connecting pipes 9, and an air conveying pipe 10 and a culture medium conveying pipe 11 are fixed on one end of the conveying pipe 8, which is positioned outside the culture box 1, and are used for conveying oxygen and culture medium into the culture components 4 respectively.

Placing lactobacillus johnsonii strain in the culture dish 41, covering the dish cover 42, placing the culture dish 41 in the placing cavity of the placing frame 33, simultaneously contacting the culture dish 41 with the inclined surface of the clamping plate 342 in the placing cavity and pressing the clamping plate 342 downwards, so that the hollow plate 341 moves inwards of the hollow plate 341 and presses the spring 343, and the clamping plate 342 clamps and fixes the culture dish 41 under the elasticity of the spring 343, thereby improving the stability of the culture dish 41 in the placing frame 33;

connecting pipes 9 to connecting pipes 43 of a plurality of culture components 4 in each placement frame 33, pushing the placement frames 3 into the incubator 1, enabling movable wheels 36 on the placement frames 33 to be clamped in wheel grooves 7 in the incubator 1 for limiting, and then connecting a conveying pipe 8 with the connecting pipes 9; then providing a required temperature for lactobacillus johnsonii strain culture by a heater 2;

in the culture process, the air conveying pipe 10 is connected with an external air pump, sterile air is injected into the culture dish 41 through the air conveying pipe 10, the conveying pipe 8, the connecting pipe 9 and the connecting pipe 43 by utilizing the air pump, the culture medium conveying pipe 11 is connected with an external conveying pump, the culture medium is injected into the culture dish 41 through the culture medium conveying pipe 11, the conveying pipe 8, the connecting pipe 9 and the connecting pipe 43 by utilizing the conveying pump, the electric push rod 5 is started to stretch simultaneously, the rack 3 drives the bottommost movable plate 32 to rotate around the rotating shaft with the bracket 31, meanwhile, the torsion spring is twisted, the bottommost movable plate 32 drives other movable plates 32 to rotate around the rotating shaft with the bracket 31 through the connecting rod 35, as shown in fig. 13, when the electric push rod 5 is retracted, the movable plate 32 is reset under the elastic force of the torsion spring, so that the shaking of the movable plates 32 can be realized through the reciprocating extension of the electric push rod 5, the culture assembly 4 on the movable plate 32 can be rocked, and the lactobacillus johnsonii strain, oxygen and the culture medium in the culture assembly 4 can be uniformly mixed, the growth and metabolism of lactobacillus johnsonii strain can be facilitated, and the propagation speed of the strain can be improved.

As shown in fig. 1-5, a ventilation assembly 6 is arranged at the rear side of the incubator 1, the ventilation assembly 6 comprises a case 61 fixed at the rear side of the incubator 1, a ventilation pipe 64 with a valve is fixed at one side of the incubator 1, air is conveniently introduced into the case 61, a plurality of sterilizing lamps 62 for sterilizing the air in the case 61 are fixed at the top end inside the case 61, a communicating pipe 63 is fixed at the front wall inside the case 61, the communicating pipe 63 is communicated with the inside of the incubator 1, the communicating pipe 63 is arranged at the bottom of the heater 2, and an electric valve is fixed on the communicating pipe 63, so that sterile air in the case 61 can enter the incubator 1.

Still be fixed with vacuum pump 12 in incubator 1 one side bottom, and the air inlet of vacuum pump 12 is fixed with exhaust tube 13, exhaust tube 13 runs through incubator 1 one side and communicates with incubator 1 inside, is convenient for take out the interior air of incubator 1. The controller at the top of one side of the incubator 1 is used for controlling the electric push rod 5, the vacuum pump 12 and the electric valve to work.

Injecting air into the ventilation assembly 6 through the ventilation pipe 64, and then sterilizing the air in the box 61 through the sterilizing lamp 62, so that the sterile air is stored in the box 61 (the sterile air introduced into the culture dish 41 can be sourced from the inside of the box 61); after pushing the rack 3 with the culture assembly 4 placed into the incubator 1, the vacuum pump 12 is used for pumping out air in the incubator 1 to enable negative pressure to be generated in the incubator 1, then an electric valve on the communicating pipe 63 is opened, sterile air in the box 61 enters the incubator 1 through the communicating pipe 63, so that air in the incubator 1 is replaced, the culture assembly 4 is cultured in a sterile environment, the probability of pollution of lactobacillus johnsonii strains in the culture assembly 4 is reduced, and the culture effect is improved.

Finally: the foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and principles of the invention are intended to be included within the scope of the invention.

Claims (10)

1. The utility model provides a lactobacillus johnsonii strain culture apparatus, includes incubator (1), inside back wall of incubator (1) is fixed with heater (2), its characterized in that: a rack (3) is arranged in the incubator (1), a plurality of culture assemblies (4) for culturing lactobacillus johnsonii strains are arranged on the rack (3), and the rack (3) is arranged at the front side of the heater (2);

the rack (3) comprises a support (31), a plurality of movable plates (32) which are distributed up and down are arranged in the support (31), centers of two sides of each movable plate (32) are rotationally connected with the support (31) through a rotating shaft, a placement frame (33) is fixed at the top end of each movable plate (32), two placement cavities are arranged in the placement frame (33), a plurality of culture components (4) are uniformly placed in the placement frames (33) of the placement cavities, connecting rods (35) are arranged at four corners between every two adjacent movable plates (32), and two ends of each connecting rod (35) are rotationally connected with the two movable plates (32) respectively;

an electric push rod (5) is fixed at the bottom end inside the incubator (1), the electric push rod (5) is arranged at the rear side of the bottom of the bottommost movable plate (32), the top end of a piston rod of the electric push rod (5) is in contact with the bottom of the movable plate (32), and a plurality of groups of movable plates (32) are driven to rotate through the expansion and contraction of the electric push rod (5), so that a culture assembly (4) on the movable plate (32) shakes, and then lactobacillus johnsonii strains, oxygen and a culture medium in the culture assembly (4) are uniformly mixed;

the rear side of the incubator (1) is provided with a ventilation assembly (6).

2. A lactobacillus johnsonii strain culture apparatus as claimed in claim 1 wherein: the culture assembly (4) comprises a culture dish (41) and a dish cover (42) arranged at the top of the culture dish (41) in a sealing cover manner, a connecting pipe (43) is fixed at the top end of the dish cover (42), the connecting pipe (43) is communicated with the inside of the culture dish (41), and a filter screen for blocking the lactobacillus johnsonii strains from entering is fixed at the bottom of the connecting pipe (43).

3. A lactobacillus johnsonii strain culture apparatus as claimed in claim 2 wherein: each of the front side wall and the rear side wall of each placing cavity is provided with a fixing component (34) for fixing the culture component (4), each group of fixing components (34) comprises a hollow plate (341) and a clamping plate (342) arranged in the hollow plate (341), one side of each clamping plate (342) extends out of the hollow plate (341), the other side of each clamping plate (342) is connected with the inner wall of the hollow plate (341) through a spring (343), and the tops of the sides of the clamping plates (342) extending out of the hollow plate (341) are processed into inclined surfaces;

the hollow plate (341) is fixed on the inner wall of the placing cavity, and two clamping plates (342) in one placing cavity are arranged on the front side and the rear side of the culture plate (41) and used for clamping and fixing the culture plate (41).

4. A lactobacillus johnsonii strain culture apparatus as claimed in claim 1 wherein: each movable plate (32) bottom four corners all is equipped with rubber pad (37), and rubber pad (37) and movable plate (32) contact, and a plurality of rubber pads (37) all are fixed in on support (31), be connected with the torsional spring between movable plate (32) and support (31), and in the pivot between movable plate (32) and support (31) is located to the torsional spring cover.

5. A lactobacillus johnsonii strain culture apparatus as claimed in claim 1 wherein: the four corners of the bottom end of the support (31) are respectively fixed with a movable wheel (36), two wheel grooves (7) are fixed at the bottom end inside the incubator (1), and the two movable wheels (36) on the same side are clamped in one wheel groove (7) for limiting the movable wheels (36), so that the stability of the support (31) is improved.

6. A lactobacillus johnsonii strain culture apparatus as claimed in claim 2 wherein: the connecting pipe (43) of multiunit cultivation subassembly (4) on every fly leaf (32) are connected through connecting pipe (9), incubator (1) one side is fixed with conveying pipeline (8), conveying pipeline (8) one end extends into incubator (1) and is connected with a plurality of connecting pipes (9), conveying pipeline (8) are located and are fixed with air conveying pipe (10) and culture medium conveyer pipe (11) on the one end outside incubator (1) for carry oxygen and culture medium in to cultivation subassembly (4) respectively.

7. A lactobacillus johnsonii strain culture apparatus as claimed in claim 1 wherein: the ventilation assembly (6) comprises a box body (61) fixed at the rear side of the incubator (1), a ventilation pipe (64) with a valve is fixed at one side of the incubator (1) so as to facilitate ventilation of air into the box body (61), and a plurality of bactericidal lamps (62) used for sterilizing the air in the box body (61) are fixed at the top end inside the box body (61).

8. The lactobacillus johnsonii strain culture apparatus of claim 7, wherein: the utility model discloses a incubator, including box (61), incubator (1), heater (2), communicating pipe (63) are located to inside front wall of box (61), communicating pipe (63) are fixed with communicating pipe (63), communicating pipe (63) are located heater (2) bottom, be fixed with the motorised valve on communicating pipe (63), the aseptic air in box (61) of being convenient for gets into in incubator (1).

9. The lactobacillus johnsonii strain culture apparatus of claim 8, wherein: the vacuum incubator is characterized in that a vacuum pump (12) is fixed at the bottom of one side of the incubator (1), an air inlet of the vacuum pump (12) is fixedly provided with an air exhaust pipe (13), and the air exhaust pipe (13) penetrates through one side of the incubator (1) and is communicated with the inside of the incubator (1) so as to conveniently exhaust air in the incubator (1).

10. A lactobacillus johnsonii strain culture apparatus as claimed in claim 9 wherein: the top of one side of the incubator (1) is fixed with a controller for controlling the heater (2), the electric push rod (5), the vacuum pump (12) and the electric valve.