CN117858885A - 核苷酸类的纯化方法和核苷酸类的纯化装置以及疏水性试剂和疏水性底物 - Google Patents

核苷酸类的纯化方法和核苷酸类的纯化装置以及疏水性试剂和疏水性底物 Download PDF

Info

Publication number
CN117858885A
CN117858885A CN202280056963.6A CN202280056963A CN117858885A CN 117858885 A CN117858885 A CN 117858885A CN 202280056963 A CN202280056963 A CN 202280056963A CN 117858885 A CN117858885 A CN 117858885A
Authority
CN
China
Prior art keywords
hydrophobic
nucleotide
protecting group
group
compound
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202280056963.6A
Other languages
English (en)
Chinese (zh)
Inventor
阿部洋
阿部奈保子
稻垣雅仁
木村康明
桥谷文贵
Z·李
中嶋裕子
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Research And Development Corp Science And Technology Revitalization Organization
Original Assignee
National Research And Development Corp Science And Technology Revitalization Organization
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Research And Development Corp Science And Technology Revitalization Organization filed Critical National Research And Development Corp Science And Technology Revitalization Organization
Publication of CN117858885A publication Critical patent/CN117858885A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/7105—Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/22—Amides of acids of phosphorus
    • C07F9/24—Esteramides
    • C07F9/2454—Esteramides the amide moiety containing a substituent or a structure which is considered as characteristic
    • C07F9/2458—Esteramides the amide moiety containing a substituent or a structure which is considered as characteristic of aliphatic amines
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
    • C07H17/02—Heterocyclic radicals containing only nitrogen as ring hetero atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/167—Purine radicals with ribosyl as the saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Saccharide Compounds (AREA)
CN202280056963.6A 2021-07-05 2022-07-05 核苷酸类的纯化方法和核苷酸类的纯化装置以及疏水性试剂和疏水性底物 Pending CN117858885A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021-111420 2021-07-05
JP2021111420 2021-07-05
PCT/JP2022/026665 WO2023282245A1 (ja) 2021-07-05 2022-07-05 ヌクレオチド類の精製方法及びヌクレオチド類の精製装置並びに疎水性試薬及び疎水性基質

Publications (1)

Publication Number Publication Date
CN117858885A true CN117858885A (zh) 2024-04-09

Family

ID=84800657

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202280056963.6A Pending CN117858885A (zh) 2021-07-05 2022-07-05 核苷酸类的纯化方法和核苷酸类的纯化装置以及疏水性试剂和疏水性底物

Country Status (5)

Country Link
US (1) US20240327444A1 (https=)
EP (1) EP4368628A4 (https=)
JP (1) JPWO2023282245A1 (https=)
CN (1) CN117858885A (https=)
WO (1) WO2023282245A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024185697A1 (ja) * 2023-03-03 2024-09-12 国立大学法人東海国立大学機構 ポリヌクレオチド連結産物の製造方法
CN116478226B (zh) * 2023-03-07 2025-08-19 江苏申基生物科技有限公司 一种锁核苷帽类似物和应用
WO2025054401A2 (en) * 2023-09-06 2025-03-13 Trilink Biotechnologies, Llc Cap analogs and methods of use thereof
CN116875658B (zh) * 2023-09-09 2024-02-06 南京鸿明生物科技有限公司 一种脱氧核酶及检测mRNA加帽率的方法
WO2025141025A2 (en) * 2023-12-26 2025-07-03 Eleven Therapeutics Ltd Novel capping strategies for mrna 5'cap
WO2025187713A1 (en) * 2024-03-07 2025-09-12 Eisai R&D Management Co., Ltd. Polynucleotide and producing method thereof
WO2026009964A1 (ja) * 2024-07-05 2026-01-08 国立大学法人東海国立大学機構 キャップ化ポリヌクレオチド
EP4725949A1 (en) * 2024-10-08 2026-04-15 BioSpring Gesellschaft für Biotechnologie mbH Improved solid-phase phosphoramidite-synthesis of long oligonucleotide molecules

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3433649A1 (de) * 1984-09-13 1986-03-20 Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig Verfahren zur aufreinigung synthetischer oligonucleotide
US5998604A (en) * 1997-09-15 1999-12-07 The Perkin-Elmer Corporation Polynucleotide purification method
US7345163B2 (en) * 2002-08-28 2008-03-18 Quiatech Ab Process for separating and deprotecting oligonucleotides
NZ590265A (en) 2008-06-11 2012-11-30 Lasergen Inc Nucleotides and nucleosides and methods for their use in dna sequencing
WO2013040257A1 (en) * 2011-09-13 2013-03-21 Lasergen, Inc. 5-methoxy. 3'-oh unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
CN102898464B (zh) * 2012-05-23 2015-04-01 北京大学 光敏感基团保护的功能基团亚磷酰胺及其制备方法和应用
US10428379B2 (en) * 2013-03-15 2019-10-01 Ibis Biosciences, Inc. Nucleotide analogs for sequencing
US10808242B2 (en) 2015-08-28 2020-10-20 Biontech Rna Pharmaceuticals Gmbh Method for reducing immunogenicity of RNA
CN120842296A (zh) 2015-09-21 2025-10-28 垂林克生物技术有限公司 用于合成5’-加帽rna的组合物和方法
EP3372610A1 (en) 2017-03-08 2018-09-12 ETH Zurich Novel phosphorylation reagents and uses thereof
JP7099190B2 (ja) 2018-08-30 2022-07-12 横浜ゴム株式会社 空気入りタイヤ
JP7551134B2 (ja) 2019-07-31 2024-09-17 国立研究開発法人科学技術振興機構 プライマー及びこれを用いた二本鎖ｄｎａの製造装置並びに二本鎖ｄｎａの製造方法

Also Published As

Publication number Publication date
EP4368628A1 (en) 2024-05-15
WO2023282245A1 (ja) 2023-01-12
JPWO2023282245A1 (https=) 2023-01-12
US20240327444A1 (en) 2024-10-03
EP4368628A4 (en) 2025-11-26

Similar Documents

Publication Publication Date Title
EP4368628A1 (en) Method for purifying nucleotides, device for purifying nucleotides, hydrophobic reagent, and hydrophobic substrate
EP4234567A1 (en) Oligonucleotide for 5'-capped rna synthesis
CN103068834B (zh) 用于反向合成rna的亚磷酰胺
DE69829760T3 (de) Bi- und tri-zyklische - nukleosid, nukleotid und oligonukleotid-analoga
EP3409780B1 (en) Nucleic acid complex
JP2021517126A (ja) ヌクレオチド前駆体、ヌクレオチド類似体およびこれを含むオリゴマー化合物
CN102971335A (zh) 可逆终止引物延伸试剂
EP3556764A1 (en) Nucleoside derivative and use therefor
CN106661075A (zh) 二磷酸和三磷酸前体药物
Lavergne et al. Synthesis and preliminary evaluation of pro-RNA 2′-O-masked with biolabile pivaloyloxymethyl groups in an RNA interference assay
FR2927901A1 (fr) Procede de preparation de nucleotides et analogues a facon par synthese sur support soluble et outils biologiques prepares
EP2854813B1 (en) Pyrazolotriazolyl nucleoside analogues and oligonucleotides comprising them
JP4802712B2 (ja) リボ核酸化合物及びオリゴ核酸化合物の液相合成法
US11110114B2 (en) Dinucleotides
WO2019195494A1 (en) Compositions and methods for synthesis of phosphorylated molecules
KR20230123318A (ko) 5'-캡핑된 rna 합성용 올리고뉴클레오티드
Bartosik et al. Access to capped RNAs by chemical ligation
AU2024351425C1 (en) Mrna cap analog and use thereof
WO1995031470A2 (en) Antisense inhibitors of gene expression
Timofeev et al. Oligodeoxynucleotides containing 2′‐deoxy‐1‐methyladenosine and Dimroth rearrangement
WO2025053250A1 (ja) タンパク質又はペプチドの製造方法、５´キャップ化ポリヌクレオチドの製造方法、及びそれらの製造方法に用いるための試薬
Moriguchi et al. Novel method of the synthesis and hybridization properties of an oligonucleotide containing non-ionic diisopropylsilyl internucleotide linkage
CN121986173A (en) Method for producing protein or peptide, method for producing 5' -capped polynucleotide, and reagent for use in these methods
TW202528539A (zh) 抑制受磷蛋白之表現的siRNA
KR20230083197A (ko) 5'-캡핑된 rna 합성용 올리고뉴클레오티드

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination