CN117844684B - Marseilles with phosphorus dissolving capability and application thereof - Google Patents
Marseilles with phosphorus dissolving capability and application thereof Download PDFInfo
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- CN117844684B CN117844684B CN202311721788.0A CN202311721788A CN117844684B CN 117844684 B CN117844684 B CN 117844684B CN 202311721788 A CN202311721788 A CN 202311721788A CN 117844684 B CN117844684 B CN 117844684B
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- massilia
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- 229910052698 phosphorus Inorganic materials 0.000 title claims abstract description 30
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 title claims abstract description 29
- 239000011574 phosphorus Substances 0.000 title claims abstract description 29
- 241001200409 Massilia arvi Species 0.000 claims abstract description 24
- 229910019142 PO4 Inorganic materials 0.000 claims abstract description 23
- 241000233866 Fungi Species 0.000 claims abstract description 19
- 239000010452 phosphate Substances 0.000 claims abstract description 19
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims abstract description 19
- 241000894006 Bacteria Species 0.000 claims abstract description 16
- 239000001506 calcium phosphate Substances 0.000 claims abstract description 7
- 229910000389 calcium phosphate Inorganic materials 0.000 claims abstract description 7
- 235000011010 calcium phosphates Nutrition 0.000 claims abstract description 7
- 239000001963 growth medium Substances 0.000 claims abstract description 7
- 238000009630 liquid culture Methods 0.000 claims abstract description 7
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims abstract description 7
- ILRRQNADMUWWFW-UHFFFAOYSA-K aluminium phosphate Chemical compound O1[Al]2OP1(=O)O2 ILRRQNADMUWWFW-UHFFFAOYSA-K 0.000 claims abstract description 6
- WBJZTOZJJYAKHQ-UHFFFAOYSA-K iron(3+) phosphate Chemical compound [Fe+3].[O-]P([O-])([O-])=O WBJZTOZJJYAKHQ-UHFFFAOYSA-K 0.000 claims abstract description 6
- 239000002367 phosphate rock Substances 0.000 claims abstract description 6
- 238000004321 preservation Methods 0.000 claims abstract 2
- 238000000855 fermentation Methods 0.000 claims description 37
- 230000004151 fermentation Effects 0.000 claims description 37
- 235000021317 phosphate Nutrition 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 12
- 239000002609 medium Substances 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 150000003013 phosphoric acid derivatives Chemical class 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 229910000398 iron phosphate Inorganic materials 0.000 claims description 2
- 239000003337 fertilizer Substances 0.000 abstract description 10
- 239000002689 soil Substances 0.000 abstract description 9
- 230000001580 bacterial effect Effects 0.000 abstract description 7
- 239000005955 Ferric phosphate Substances 0.000 abstract description 4
- 229940032958 ferric phosphate Drugs 0.000 abstract description 4
- 229910052816 inorganic phosphate Inorganic materials 0.000 abstract description 4
- 229910000399 iron(III) phosphate Inorganic materials 0.000 abstract description 4
- 239000000843 powder Substances 0.000 abstract description 4
- 230000000813 microbial effect Effects 0.000 abstract description 3
- 244000005700 microbiome Species 0.000 description 6
- 239000000243 solution Substances 0.000 description 4
- 238000012163 sequencing technique Methods 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 241000235342 Saccharomycetes Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000002686 phosphate fertilizer Substances 0.000 description 2
- -1 phosphokalium-aluminum Chemical compound 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 230000003381 solubilizing effect Effects 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- WYWFMUBFNXLFJK-UHFFFAOYSA-N [Mo].[Sb] Chemical compound [Mo].[Sb] WYWFMUBFNXLFJK-UHFFFAOYSA-N 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- KMQAPZBMEMMKSS-UHFFFAOYSA-K calcium;magnesium;phosphate Chemical compound [Mg+2].[Ca+2].[O-]P([O-])([O-])=O KMQAPZBMEMMKSS-UHFFFAOYSA-K 0.000 description 1
- YYRMJZQKEFZXMX-UHFFFAOYSA-N calcium;phosphoric acid Chemical compound [Ca+2].OP(O)(O)=O.OP(O)(O)=O YYRMJZQKEFZXMX-UHFFFAOYSA-N 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 208000037824 growth disorder Diseases 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 1
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000002426 superphosphate Substances 0.000 description 1
- 239000013585 weight reducing agent Substances 0.000 description 1
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- Fertilizers (AREA)
Abstract
The invention discloses a mosaic bacterium with phosphorus dissolving capability and application thereof, wherein the preservation number of the mosaic bacterium (MASSILIA ARVI P) is CCTCC NO: m20232231. The strain MASSILIA ARVI P of the invention has the capacity of dissolving indissolvable phosphate, can convert indissolvable inorganic phosphate into soluble phosphorus which can be directly absorbed and utilized by plants, and has the phosphorus dissolving amount of 45.2 mug mL ‑1、5.9μg mL‑1、6.9μg mL‑1、4.2μg mL‑1 respectively after being cultured in four indissolvable phosphate liquid culture media of calcium phosphate, ferric phosphate, aluminum phosphate and phosphate rock powder for 7 days. The invention provides a certain reference for the later research of wild strains by combining the phosphorus dissolving capability of the mosaic fungus MASSILIA ARVI P and the soil for the first time, and has important value for the development of microbial fertilizers such as phosphate dissolving bacterial fertilizers and the like.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to a mosaic bacterium with phosphorus dissolving capability and application thereof.
Background
Phosphorus is one of the most important nutrient elements required for plant growth, and is a component of many important organic compounds in plants, and is involved in various metabolic processes in plants in various ways. Phosphorus deficiency generally results in slow plant growth, stunting, poor fruiting and delayed ripening, resulting in reduced fruit yield and quality. The phosphorus in the inorganic phosphate fertilizer for traditional agriculture is usually fixed by the action of active iron and aluminum in soil solution, and then is hydrolyzed and converted into phosphate such as phosphokalium-aluminum and phosphokalium-iron-aluminum, and further converted into closed phosphate, for example, 80-90% of superphosphate and calcium magnesium phosphate fertilizer contained in the common phosphate fertilizer can be converted into Fe-P and Al-P forms in red soil dry land and cannot be utilized by plants. And a part of microorganisms can promote the conversion of ineffective phosphorus in soil into effective phosphorus which can be absorbed and utilized by plants, and the microorganisms are called soil phosphorus conversion microorganisms, also called phosphorus dissolving microorganisms, wherein bacteria are called phosphorus dissolving bacteria or phosphorus dissolving bacteria, and if the microorganisms can be applied to phosphate fertilizers, the efficiency of the phosphate fertilizers can be effectively improved, and the weight reduction and the synergy of the phosphate fertilizers are realized.
Disclosure of Invention
The invention aims to provide a mosaic bacterium with phosphorus dissolving capability and application thereof.
In order to achieve the aim of the invention, the invention adopts the following technical scheme:
in a first aspect, a mosaic bacterium (MASSILIA ARVI P) having a phosphorus-dissolving capability is deposited in China center for type culture collection (CCTCC NO: m20232231.
In a second aspect, there is provided a dissolving agent containing mosaic fungus MASSILIA ARVI P, which comprises a mosaic fungus fermentation broth, or a mosaic fungus aseptic fermentation broth.
Further, the preparation method of the mosaic fermentation broth comprises the following steps:
1) Preparing seed liquid: inoculating mosaic fungus MASSILIA ARVI P to a modified PKO liquid culture medium, culturing at 28 ℃ for 5 to 7 days at 240r/min, and preparing seed liquid;
2) Fermentation: inoculating the seed liquid into a fermentation medium, wherein the fermentation conditions are as follows: the inoculation amount is 1vol%, the bottling amount is 200mL/250mL, the initial pH value of the fermentation medium is 7.0-7.2, the temperature is 28 ℃, the fermentation time is 180r/min, and the fermentation time is 10 days, so that the mosaic fermentation liquid is obtained.
Further, the preparation method of the mosaic bacteria aseptic fermentation broth comprises the following steps:
Centrifuging the mosaic fungus fermentation liquor for 5min at 5000r/min, and filtering and sterilizing by using a 0.4 mu m sterilizing microporous filter membrane to obtain the mosaic fungus aseptic fermentation liquor.
In a third aspect, an application of mosaic fungus MASSILIA ARVI P2 in dissolving insoluble phosphate is provided.
Further, the poorly soluble phosphate includes, but is not limited to, one or more of calcium phosphate, iron phosphate, aluminum phosphate, and ground phosphate rock.
In a fourth aspect, there is provided the use of a lytic agent comprising mosaic bacteria MASSILIA ARVI P for the lysis of poorly soluble phosphates.
The beneficial effects of the invention are as follows:
The strain MASSILIA ARVI P of the invention has the capacity of dissolving indissolvable phosphate, can convert indissolvable inorganic phosphate into soluble phosphorus which can be directly absorbed and utilized by plants, and has the phosphorus dissolving amount of 45.2 mug mL -1、5.9μg mL-1、6.9μg mL-1、4.2μg mL-1 respectively after being cultured in four indissolvable phosphate liquid culture media of calcium phosphate, ferric phosphate, aluminum phosphate and phosphate rock powder for 7 days. The invention provides a certain reference for the later research of wild strains by combining the mosaic fungus MASSILIA ARVI with the soil phosphorus dissolving capability for the first time, and has important value for developing microbial fertilizers such as phosphate dissolving bacterial fertilizers.
Detailed Description
The following description of the embodiments of the present invention is provided to facilitate understanding of the present invention by those skilled in the art, but it should be understood that the present invention is not limited to the scope of the embodiments, and all the inventions which make use of the inventive concept are protected by the spirit and scope of the present invention as defined and defined in the appended claims to those skilled in the art.
Example 1 isolation and identification of Male Saccharomycetes MASSILIA ARVI P2
The method for separating the mosaic fungus MASSILIA ARVI comprises the following steps:
1g of soil sample is weighed under sterile environment, placed in a triangular flask (with sterile glass beads inside) filled with 9mL of sterile water, oscillated for 30min at 28 ℃ for 160 r.min, 3 continuous dilutions (10 -4,10-5,10-6) are selected, and inoculated on modified PKO flat-plate medium by a surface coating method.
Each plate was inoculated with 0.1ml of soil solution. The inoculated plates were incubated in an incubator at 28℃for about 4d. After the colonies grow out, dominant colonies are further screened according to the growth conditions and the size of the colonies.
The strain of the present transparent phosphate solubilizing ring was picked up, streaked continuously 3 times, and only the strain which could grow on PKO plates after 3 times of continuous streaked culture was considered as effective phosphate solubilizing bacteria.
Preferably, the modified PKO medium comprises 3g of calcium phosphate, 10g of sucrose, 0.5g of ammonium sulfate, 0.1g of sodium chloride, 0.1g of magnesium sulfate heptahydrate, 0.2g of potassium chloride, 0.03g of manganese sulfate, 0.03g of ferrous sulfate heptahydrate, 0.5g of saccharomycete paste, 15g of agar, 1000mL of water and pH of 7.0-7.2.
Selecting a purified and cultured strain, and sequencing by 16SrDNA, wherein the sequencing sequence is as follows:
ACATGCAGTCGAACGGCAGCGCGGGGCAACCTGGCGGCGAGTGGCGAACGGGTGAGTAATACATCGGAACGTACCCAGAAGTGGGGGATAACGTAGCGAAAGTTACGCTAATACCGCATACGTTCTACGGAAGAAAGTGGGGGATCTTCGGACCTCATGCTTTTGGAGCGGCCGATGTCTGATTAGCTAGTTGGTGAGGTAAAGGCTCACCAAGGCGACGATCAGTAGCTGGTCTGAGAGGACGACCA
GCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGT
GGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCAATGCCGCGTGAG
TGAAGAAGGCCTTCGGGTTGTAAAGCTCTTTTGTCAGGGAAGAAACGGTT
CGGGCTAATATCCGGGGCTAATGACGGTACCTGAAGAATAAGCACCGGCTA
ACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGA
ATTACTGGGCGTAAAGCGTGCGCAGGCGGTTTTGTAAGTCTGACGTGAAAT
CCCCGGGCTTAACCTGGGAATTGCGTTGGAGACTGCAAGGCTGGAGTCTG
GCAGAGGGGGGTAGAATTCCACGTGTAGCAGTGAAATGCGTAGAGATGTG
GAGGAACACCGATGGCGAAGGCAGCCCCCTGGGTCAAGACTGACGCTCAT
GCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGC
CCTAAACGATGTCTACTAGTTGTCGGGTCTTAATTGACTTGGTAACGCAGCT
AACGCGTGAAGTAGACCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCA
AAGGAATTGACGGGGACCCGCACAAGCGGTGGATGATGTGGATTAATTCG
ATGCAACGCGAAAAACCTTACCTACCCTTGACATGTCAGGAACCTCCGAG
AGATCGGAGGGTGCCCGAAAGGGAGCCTGAACACAGGTGCTGCATGGCT
GTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCA
ACCCTTGTCATTAGTTGCTACGAAAGGGCACTCTAATGAGACTGCCGGTGA
CAAACCGGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCCTTATGGG
TAGGGCTTCACACGTCATACAATGGTACATACAGAGGGCCGCCAACCCGCG
AGGGGGAGCTAATCCCAGAAAGTGTATCGTAGTCCGGATCGCAGTCTGCA
ACTCGACTGCGTGAAGTTGGAATCGCTAGTAATCGCGGATCAGCATGCCGC
GGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGC
GGGTTTTACCAGAAGTAGGTAGCTTAACCGCAAGGAGGGCGCtTACCACGGTAGGATA。
and comparing the sequences obtained by sequencing in NCBI, and selecting the sequence with the maximum similarity as a species identification result. The similarity between the sequence and the 16SrDNA gene sequence of mosaic bacteria MASSILIA ARVI is up to 99.01%.
Example 2 preparation of Marseilles MASSILIA ARVI P2 fermentation broth
1) Preparing seed liquid: inoculating mosaic fungus MASSILIA ARVI P to a modified PKO liquid culture medium, culturing at 28 ℃ for 5 to 7 days at 240r/min, and preparing seed liquid;
2) Fermentation: inoculating the seed liquid into a fermentation medium, wherein the fermentation conditions are as follows: the inoculation amount is 1vol%, the bottling amount is 200mL/250mL, the initial pH value of the fermentation medium is 7.0-7.2, the temperature is 28 ℃, the fermentation time is 180r/min, and the fermentation time is 10 days, so as to obtain the mosaic fungus MASSILIA ARVI P fermentation broth.
EXAMPLE 3 preparation of sterile fermentation broth of Marseilles MASSILIA ARVI P2
Centrifuging the mosaic fungus MASSILIA ARVI P fermentation liquor obtained in the example 2 for 5min at 5000r/min, and filtering and sterilizing with a 0.4 μm sterilizing microporous filter membrane to obtain the mosaic fungus aseptic fermentation liquor.
Example 4 use of mosaic bacteria MASSILIA ARVI P2 in dissolving poorly soluble phosphates
The strain MASSILIA ARVI P has the capacity of dissolving indissolvable phosphate, can convert indissolvable inorganic phosphate into soluble phosphorus which can be directly absorbed and utilized by plants, is inoculated with bacterial liquid (OD 600 is 0.5) prepared by the same amount of strain into PKO liquid culture medium taking calcium phosphate, ferric phosphate, aluminum phosphate and phosphate rock powder as phosphorus sources, is placed in a constant-temperature shaking table (160 r/min,28 ℃) for 7d, is centrifuged (10000 r/min) at 4 ℃ for 15min, and the effective phosphorus content of the supernatant is measured by a molybdenum-antimony colorimetric method, and is also compared with a non-inoculated bacterial suspension.
The calculation formula is as follows: p= (K-Kck) x V/V1
Wherein: p is the phosphorus dissolving amount (mug/mL); k is the content of available phosphorus measured by the bacterial culture solution to be measured; kck is the available phosphorus content (μg/mL) measured without inoculating the bacterial suspension; v is the volume (mL) of the fixed volume of the solution during color development; v1 is the volume of supernatant aspirated (mL) during development.
The result shows that the phosphorus dissolving amount of the mosaic fungus MASSILIA ARVI cultured in four indissolvable phosphate liquid culture media of calcium phosphate, ferric phosphate, aluminum phosphate and phosphate rock powder for 7 days is 45.224±7.067μg mL-1、5.907±4.422μg mL-1、6.881±3.137μg mL-1、4.189±5.335μg mL-1. respectively, and the invention combines the phosphorus dissolving capacity of the mosaic fungus MASSILIA ARVI and soil for the first time, provides a certain reference for later research of wild strains, and has important value for development of microbial fertilizers such as phosphate dissolving bacterial fertilizers.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.
Claims (7)
1. A mosaic bacterium (MASSILIA ARVI P) with phosphorus dissolving capability, which is characterized in that the preservation number of the mosaic bacterium is CCTCC NO: m20232231.
2. A dissolving agent comprising the mosaic bacteria of claim 1, wherein the dissolving agent comprises a fermentation broth of mosaic bacteria or a sterile fermentation broth of mosaic bacteria.
3. The dissolving agent according to claim 2, characterized in that the preparation method of the mosaic fermentation broth comprises the following steps:
1) Preparing seed liquid: inoculating mosaic fungus MASSILIA ARVI P to a modified PKO liquid culture medium, culturing at 28 ℃ for 5 to 7 days at 240r/min, and preparing seed liquid;
2) Fermentation: inoculating the seed liquid into a fermentation medium, wherein the fermentation conditions are as follows: the inoculation amount is 1vol%, the bottling amount is 200mL/250mL, the initial pH value of the fermentation medium is 7.0-7.2, the temperature is 28 ℃, the fermentation time is 180r/min, and the fermentation time is 10 days, so that the mosaic fermentation liquid is obtained.
4. A dissolving agent according to claim 3, wherein the preparation method of the mosaic bacteria aseptic body fermentation broth comprises the following steps:
Centrifuging the mosaic fungus fermentation liquor for 5min at 5000r/min, and filtering and sterilizing by using a 0.4 mu m sterilizing microporous filter membrane to obtain the mosaic fungus aseptic fermentation liquor.
5. Use of the mosaic of claim 1 for dissolving poorly soluble phosphates.
6. The use according to claim 5, wherein the poorly soluble phosphate comprises one or more of calcium phosphate, iron phosphate, aluminum phosphate, ground phosphate rock.
7. Use of a dissolving agent according to any one of claims 2-4 for dissolving poorly soluble phosphates.
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| CN110317744A (en) * | 2019-05-08 | 2019-10-11 | 安徽农业大学 | A method of producing Marseille bacterium and its production basket purpurin of Indigo pigment |
| CN111500494A (en) * | 2020-04-25 | 2020-08-07 | 甘肃省科学院生物研究所 | Thymus damascena with high enzyme activity and screening method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110317744A (en) * | 2019-05-08 | 2019-10-11 | 安徽农业大学 | A method of producing Marseille bacterium and its production basket purpurin of Indigo pigment |
| CN111500494A (en) * | 2020-04-25 | 2020-08-07 | 甘肃省科学院生物研究所 | Thymus damascena with high enzyme activity and screening method and application thereof |
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