CN117603857A - Copper bacteria with phosphorus dissolving capability and application thereof - Google Patents
Copper bacteria with phosphorus dissolving capability and application thereof Download PDFInfo
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- CN117603857A CN117603857A CN202311562215.8A CN202311562215A CN117603857A CN 117603857 A CN117603857 A CN 117603857A CN 202311562215 A CN202311562215 A CN 202311562215A CN 117603857 A CN117603857 A CN 117603857A
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- phosphate
- fermentation
- copper
- bacteria
- dissolving
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- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 title claims abstract description 42
- 229910052802 copper Inorganic materials 0.000 title claims abstract description 42
- 239000010949 copper Substances 0.000 title claims abstract description 42
- 241000894006 Bacteria Species 0.000 title claims abstract description 29
- 229910052698 phosphorus Inorganic materials 0.000 title claims abstract description 23
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 title claims abstract description 22
- 239000011574 phosphorus Substances 0.000 title claims abstract description 22
- 241000252867 Cupriavidus metallidurans Species 0.000 claims abstract description 21
- 229910019142 PO4 Inorganic materials 0.000 claims abstract description 20
- 239000010452 phosphate Substances 0.000 claims abstract description 18
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims abstract description 18
- 239000001506 calcium phosphate Substances 0.000 claims abstract description 6
- 229910000389 calcium phosphate Inorganic materials 0.000 claims abstract description 6
- 235000011010 calcium phosphates Nutrition 0.000 claims abstract description 6
- 239000001963 growth medium Substances 0.000 claims abstract description 6
- 238000009630 liquid culture Methods 0.000 claims abstract description 6
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims abstract description 6
- ILRRQNADMUWWFW-UHFFFAOYSA-K aluminium phosphate Chemical compound O1[Al]2OP1(=O)O2 ILRRQNADMUWWFW-UHFFFAOYSA-K 0.000 claims abstract description 5
- WBJZTOZJJYAKHQ-UHFFFAOYSA-K iron(3+) phosphate Chemical compound [Fe+3].[O-]P([O-])([O-])=O WBJZTOZJJYAKHQ-UHFFFAOYSA-K 0.000 claims abstract description 5
- 239000002367 phosphate rock Substances 0.000 claims abstract description 5
- 238000004321 preservation Methods 0.000 claims abstract description 4
- 238000000855 fermentation Methods 0.000 claims description 37
- 230000004151 fermentation Effects 0.000 claims description 37
- 239000007788 liquid Substances 0.000 claims description 11
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 239000002609 medium Substances 0.000 claims description 7
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 229910000398 iron phosphate Inorganic materials 0.000 claims description 2
- 235000021317 phosphate Nutrition 0.000 claims 3
- 150000003013 phosphoric acid derivatives Chemical class 0.000 claims 2
- 230000002101 lytic effect Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000003337 fertilizer Substances 0.000 abstract description 9
- 239000002689 soil Substances 0.000 abstract description 9
- 239000005955 Ferric phosphate Substances 0.000 abstract description 3
- 230000001580 bacterial effect Effects 0.000 abstract description 3
- 229940032958 ferric phosphate Drugs 0.000 abstract description 3
- 229910000399 iron(III) phosphate Inorganic materials 0.000 abstract description 3
- 230000000813 microbial effect Effects 0.000 abstract description 3
- 239000000843 powder Substances 0.000 abstract description 3
- 210000003608 fece Anatomy 0.000 abstract 1
- 239000010871 livestock manure Substances 0.000 abstract 1
- 244000005700 microbiome Species 0.000 description 6
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000002686 phosphate fertilizer Substances 0.000 description 2
- -1 phosphokalium-aluminum Chemical compound 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 230000003381 solubilizing effect Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- KMQAPZBMEMMKSS-UHFFFAOYSA-K calcium;magnesium;phosphate Chemical compound [Mg+2].[Ca+2].[O-]P([O-])([O-])=O KMQAPZBMEMMKSS-UHFFFAOYSA-K 0.000 description 1
- YYRMJZQKEFZXMX-UHFFFAOYSA-N calcium;phosphoric acid Chemical compound [Ca+2].OP(O)(O)=O.OP(O)(O)=O YYRMJZQKEFZXMX-UHFFFAOYSA-N 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 208000037824 growth disorder Diseases 0.000 description 1
- 229910052816 inorganic phosphate Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 1
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000002426 superphosphate Substances 0.000 description 1
- 239000013585 weight reducing agent Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Materials Engineering (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a copper bacteria with phosphorus dissolving capability and application thereof, wherein the preservation number of the copper bacteria P4 (Cupriavidus metallidurans P4) is CCTCC NO: m20231864. The strain Cupriavidus metallidurans P of the invention has the capacity of dissolving indissolvable phosphate, and the phosphorus dissolving amount of the strain is 4.1 mug mL respectively after the strain is cultured in four indissolvable phosphate liquid culture media of calcium phosphate, ferric phosphate, aluminum phosphate and rock phosphate powder for 7 days ‑1 、36.0μgmL ‑1 、33.4μg mL ‑1 、2.7μg mL ‑1 . For the first time, combining the copper bacteria Cupriavidus metallidurans P4 with the phosphorus dissolving capability of soil, is wildThe strain later-stage research provides a certain reference and has important value for the development of phosphate-dissolving bacterial manure microbial fertilizer.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to a copper bacteria with phosphorus dissolving capability and application thereof.
Background
Phosphorus is one of the most important nutrient elements required for plant growth, and is a component of many important organic compounds in plants, and is involved in various metabolic processes in plants in various ways. Phosphorus deficiency generally results in slow plant growth, stunting, poor fruiting and delayed ripening, resulting in reduced fruit yield and quality. The phosphorus in the inorganic phosphate fertilizer for traditional agriculture is usually fixed by the action of active iron and aluminum in soil solution, and then is hydrolyzed and converted into phosphate such as phosphokalium-aluminum and phosphokalium-iron-aluminum, and further converted into closed phosphate, for example, 80-90% of superphosphate and calcium magnesium phosphate fertilizer contained in the common phosphate fertilizer can be converted into Fe-P and Al-P forms in red soil dry land and cannot be utilized by plants. And a part of microorganisms can promote the conversion of ineffective phosphorus in soil into effective phosphorus which can be absorbed and utilized by plants, and the microorganisms are called soil phosphorus conversion microorganisms, also called phosphorus dissolving microorganisms, wherein bacteria are called phosphorus dissolving bacteria or phosphorus dissolving bacteria, and if the microorganisms can be applied to phosphate fertilizers, the efficiency of the phosphate fertilizers can be effectively improved, and the weight reduction and the synergy of the phosphate fertilizers are realized.
Disclosure of Invention
The invention aims to provide a copper bacteria with phosphorus dissolving capability and application thereof.
In order to achieve the aim of the invention, the invention adopts the following technical scheme:
the utility model provides a copper fungus genus P4 (Cupriavidus metallidurans P) with phosphorus dissolving capability, which is preserved in China center for type culture Collection (China) for 10 months and 12 days in 2023, wherein the preservation address is China, university of Wuhan, and the preservation number is CCTCC NO: m20231864.
A dissolving agent containing copper greedy Cupriavidus metallidurans P4 is provided, which comprises a copper greedy fermentation broth, or a copper greedy aseptic fermentation broth.
Further, the preparation method of the copper bacteria fermentation broth comprises the following steps:
1) Preparing seed liquid: inoculating the copper bacteria Cupriavidus metallidurans P4 into an improved PKO liquid culture medium, culturing at 28 ℃ for 240r/min for 5-7 d, and preparing seed liquid;
2) Fermentation: inoculating the seed liquid into a fermentation medium, wherein the fermentation conditions are as follows: the inoculation amount is 1vol%, the bottling amount is 200mL/250mL, the initial pH value of the fermentation medium is 7.0-7.2, the temperature is 28 ℃, the fermentation time is 180r/min, and the fermentation time is 10 days, so that the copper bacteria fermentation liquid is obtained.
Further, the preparation method of the copper greedy bacteria aseptic fermentation broth comprises the following steps:
centrifuging the copper bacteria fermentation liquor at 5000r/min for 5min, and filtering and sterilizing by using a 0.4 mu m sterilizing microporous filter membrane to obtain the copper bacteria aseptic fermentation liquor.
An application of the copper bacteria Cupriavidus metallidurans P4 in dissolving insoluble phosphate is provided.
Further, the poorly soluble phosphate includes, but is not limited to, one or more of calcium phosphate, iron phosphate, aluminum phosphate, and ground phosphate rock.
There is provided the use of a dissolving agent comprising a copper bacterium for dissolving a poorly soluble phosphate.
The beneficial effects of the invention are as follows:
the strain Cupriavidus metallidurans P of the invention has the capacity of dissolving indissolvable phosphate, and the phosphorus dissolving amount of the strain is 4.1 mug mL respectively after the strain is cultured in four indissolvable phosphate liquid culture media of calcium phosphate, ferric phosphate, aluminum phosphate and rock phosphate powder for 7 days -1 、36.0μg mL -1 、33.4μg mL -1 、2.7μg mL -1 . The method provides a certain reference for the combination of the copper bacteria Cupriavidus metallidurans P4 and the soil phosphorus dissolving capability for the first time and the later research of the wild strain, and has important value for the development of microbial fertilizers such as phosphate dissolving bacterial fertilizers.
Detailed Description
The following description of the embodiments of the present invention is provided to facilitate understanding of the present invention by those skilled in the art, but it should be understood that the present invention is not limited to the scope of the embodiments, and all the inventions which make use of the inventive concept are protected by the spirit and scope of the present invention as defined and defined in the appended claims to those skilled in the art.
EXAMPLE 1 isolation and identification of copper greedy Cupriavidus metallidurans P4
A method for separating copper bacteria Cupriavidus metallidurans P4, comprising the steps of:
1g of soil sample is weighed under sterile environment, placed in a triangular flask (with sterile glass beads inside) filled with 9mL of sterile water, oscillated for 30min at 28 ℃ for 160 r.min, and 3 continuous dilutions (10 -4 ，10 -5 ，10 -6 ) The modified PKO plates were inoculated with surface smear.
Each plate was inoculated with 0.1ml of soil solution. The inoculated plates were incubated in an incubator at 28℃for about 4d. After the colonies grow out, dominant colonies are further screened according to the growth conditions and the size of the colonies.
The strain of the present transparent phosphate solubilizing ring was picked up, streaked continuously 3 times, and only the strain which could grow on PKO plates after 3 times of continuous streaked culture was considered as effective phosphate solubilizing bacteria.
Preferably, the modified PKO medium comprises 3g of calcium phosphate, 10g of sucrose, 0.5g of ammonium sulfate, 0.1g of sodium chloride, 0.1g of magnesium sulfate heptahydrate, 0.2g of potassium chloride, 0.03g of manganese sulfate, 0.03g of ferrous sulfate heptahydrate, 0.5g of saccharomycete paste, 15g of agar, 1000mL of water and pH of 7.0-7.2.
Selecting purified and cultured strains, sequencing by 16SrDNA, comparing sequences obtained by sequencing in NCBI, and selecting the sequence with the maximum similarity as a species identification result. The similarity of the sequence with the sequence of the 16SrDNA gene of the copper bacteria (Cupriavidus metallidurans) is up to 98.80 percent through comparison.
EXAMPLE 2 preparation of fermentation broth of copper greedy Cupriavidus metallidurans P4
1) Preparing seed liquid: inoculating the copper bacteria Cupriavidus metallidurans P4 into an improved PKO liquid culture medium, culturing at 28 ℃ for 240r/min for 5-7 d, and preparing seed liquid;
2) Fermentation: inoculating the seed liquid into a fermentation medium, wherein the fermentation conditions are as follows: the inoculation amount is 1vol%, the bottling amount is 200mL/250mL, the initial pH value of the fermentation medium is 7.0-7.2, the temperature is 28 ℃, the fermentation time is 180r/min, and the fermentation time is 10 days, so that the copper bacteria Cupriavidus metallidurans P fermentation liquor is obtained.
EXAMPLE 3 preparation of copper greedy Cupriavidus metallidurans P4 aseptic fermentation broth
After the copper bacteria Cupriavidus metallidurans P fermentation broth obtained in example 2 was centrifuged at 5000r/min for 5min, the copper bacteria sterile fermentation broth was obtained by filtration sterilization with a 0.4 μm sterilizing microporous filter membrane.
The strain Cupriavidus metallidurans P of the invention has the capacity of dissolving indissolvable phosphate, and the phosphorus dissolving amount of the strain is 4.1 mug mL respectively after the strain is cultured in four indissolvable phosphate liquid culture media of calcium phosphate, ferric phosphate, aluminum phosphate and rock phosphate powder for 7 days -1 、36.0μg mL -1 、33.4μg mL -1 、2.7μg mL -1 . The invention provides a certain reference for the later research of wild strains by combining the capacity of dissolving phosphorus of the copper bacteria Cupriavidus metallidurans P and soil for the first time, and has important value for the development of microbial fertilizers such as phosphate-dissolving bacterial fertilizers and the like.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.
Claims (7)
1. Copper bacteria (Cupriavidus metallidurans P4) with phosphorus dissolving capability, wherein the copper bacteria P4 has a preservation number of CCTCC NO: m20231864.
2. A dissolving agent comprising the copper bacteria of claim 1, wherein the dissolving agent comprises a copper bacteria fermentation broth, or a copper bacteria aseptic fermentation broth.
3. The dissolving agent according to claim 2, characterized in that the process for preparing a fermentation broth of copper bacteria comprises the following steps:
1) Preparing seed liquid: inoculating the copper bacteria Cupriavidus metallidurans P4 into an improved PKO liquid culture medium, culturing at 28 ℃ for 240r/min for 5-7 d, and preparing seed liquid;
2) Fermentation: inoculating the seed liquid into a fermentation medium, wherein the fermentation conditions are as follows: the inoculation amount is 1vol%, the bottling amount is 200mL/250mL, the initial pH value of the fermentation medium is 7.0-7.2, the temperature is 28 ℃, the fermentation time is 180r/min, and the fermentation time is 10 days, so that the copper bacteria fermentation liquid is obtained.
4. A lytic agent according to claim 3, wherein the preparation method of the copper bacteria aseptic body fermentation broth comprises the steps of:
centrifuging the copper bacteria fermentation liquor at 5000r/min for 5min, and filtering and sterilizing by using a 0.4 mu m sterilizing microporous filter membrane to obtain the copper bacteria aseptic fermentation liquor.
5. Use of the copper bacteria of claim 1 for dissolving poorly soluble phosphates.
6. The use according to claim 5, wherein the poorly soluble phosphate comprises one or more of calcium phosphate, iron phosphate, aluminum phosphate, ground phosphate rock.
7. Use of a dissolving agent according to any one of claims 2-4 for dissolving poorly soluble phosphates.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311562215.8A CN117603857A (en) | 2023-11-22 | 2023-11-22 | Copper bacteria with phosphorus dissolving capability and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311562215.8A CN117603857A (en) | 2023-11-22 | 2023-11-22 | Copper bacteria with phosphorus dissolving capability and application thereof |
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| CN117603857A true CN117603857A (en) | 2024-02-27 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113846027A (en) * | 2021-06-29 | 2021-12-28 | 湖南人文科技学院 | Metal-tolerant cuprum strain and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113846027A (en) * | 2021-06-29 | 2021-12-28 | 湖南人文科技学院 | Metal-tolerant cuprum strain and application thereof |
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