CN117821358B - Fermentation method of pine and red plum extract and application thereof - Google Patents
Fermentation method of pine and red plum extract and application thereof Download PDFInfo
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Abstract
The invention relates to the technical field of skin care product raw materials, and particularly discloses a fermentation method of a pine and red plum extract and application thereof. The fermentation method comprises the following steps: 1) Preparing a liquid culture medium; 2) Inoculating composite bacteria in the liquid culture medium, and culturing until the OD value is more than or equal to 1 to obtain bacterial suspension; 3) Preparing an extracting solution, soaking the truffle plum blossom in the extracting solution, then adding a bacterial suspension, and fermenting for 5-7d; 4) Heating and extracting; 5) Filtering to obtain filtrate; 6) Concentrating the filtrate, and drying to obtain extract of flos Pini Massonianae; the liquid culture medium is a compound of solvent, crab apple juice, lantern fruit juice, white melon juice, sweet potato leaf juice, monopotassium phosphate, magnesium sulfate, calcium chloride, zinc sulfate and ferric citrate; the compound bacteria are the compound of staphylococcus equi, sea fiber monad and coriolus versicolor; the extracting solution is a compound of solvent, potassium dihydrogen phosphate, magnesium sulfate, calcium chloride, zinc sulfate and ferric citrate. The invention has the advantage of quick anti-allergy and relieving effects.
Description
Technical Field
The invention relates to the field of skin care product raw materials, in particular to a fermentation method of a pine and red plum extract and application thereof.
Background
The skin care product is a product for protecting skin, and has good effects of improving skin and improving qi.
In the current fast-paced life, people are more or less injured due to various reasons such as irregular work and rest, excessive use of makeup products and the like, and allergic phenomena such as skin redness, swelling, itching and the like can occur partially seriously, so that skin care products specially used for resisting allergy and relieving are needed to improve the skin in a targeted manner, and the skin is healthier.
The skin care product for anti-allergy repair is usually prepared by adding raw materials with effects of calming, relieving itching, diminishing inflammation, relieving pain and the like to realize the effects of anti-allergy relief, as the green natural concept is continuously in deep into the heart of a person, more and more plant extracts are applied to the skin care product, the pine red plum extract is one of the existing extracts with the effects of calming, relieving itching, diminishing inflammation, relieving pain and the like, and is usually used for preparing the skin care product for anti-allergy relief, but the existing plant extracts are mainly prepared by a high-temperature water extraction method, active ingredients of the pine red plum extract are easily damaged under the action of high temperature in the extraction process of the pine red plum extract, and the addition amount of the plant extracts is lower in the skin care product, so that the effect of anti-allergy relief generated after the pine red plum extract prepared by the existing method is applied to the skin care product is more general, the effect of fast anti-allergy relief is difficult to play, and the consumer needs are difficult to be satisfied, and therefore, the room for improvement is still provided.
Disclosure of Invention
In order to play a role in quick anti-allergy and relieving, the application provides a fermentation method of a pine-red plum extract and application thereof.
In a first aspect, the application provides a fermentation method of a pine-red plum extract, which adopts the following technical scheme:
a fermentation method of a pine-red plum extract, comprising the steps of:
Step 1), preparing a liquid culture medium;
step 2), inoculating composite bacteria in the liquid culture medium, and culturing until the OD value is more than or equal to 1 to obtain bacterial suspension;
Step 3), preparing an extracting solution, soaking the truffle plum blossom in the extracting solution, then adding a bacterial suspension, and fermenting for 5-7d;
Step 4), heating and extracting;
Step 5), filtering to obtain filtrate;
step 6), concentrating and drying the filtrate to obtain a pine and red plum extract;
The liquid culture medium is a compound of a solvent, crab apple juice, lantern fruit juice, white melon juice, sweet potato leaf juice, monopotassium phosphate, magnesium sulfate, calcium chloride, zinc sulfate and ferric citrate;
the compound bacteria are the compound of staphylococcus equi, sea fiber monad and coriolus versicolor;
the extracting solution is a compound of solvent, potassium dihydrogen phosphate, magnesium sulfate, calcium chloride, zinc sulfate and ferric citrate.
By adopting the technical scheme, under the mutual coordination of staphylococcus equi, sea fiber monad and coriolus versicolor, the fermentation effect is better, so that active ingredients in cells can be better dissolved in a solvent, the extraction effect of the active ingredients is better, the content of the active ingredients in the extract is higher, and the anti-allergy relieving effect of the pine and red plum extract is faster.
The crabapple juice, the lantern juice, the white melon juice and the sweet potato leaf juice are added into the liquid culture medium, so that the composite bacteria can play a natural screening effect in the reproduction process of the composite bacteria in the special liquid culture medium, the composite bacteria in the bacterial suspension formed by culture have stronger decomposition function on the cell wall of the pine-red plum, the efficiency of damaging the cell wall is higher, the composite bacteria are reproduced in the special extracting solution by throwing the bacterial suspension into the extracting solution, the nutrition components in the extracting solution are continuously consumed while the cell wall of the pine-red plum is decomposed, special metabolic substances can be generated through the coordination of staphylococcus equi, sea-fiber monad and coriolus versicolor, the irritation substances are not easily generated in the subsequent high-temperature extraction process, the prepared pine-red plum extract has higher safety, the skin care products added with the pine-red plum extract are not easily damaged for the second time to the skin, the anti-red plum active components of the pine-red plum are easily counteracted, the quick-sensitive ingredients are quickly damaged, the anti-allergic effect is well reduced, and the anti-allergic effect is better realized, and the anti-allergic effect is better satisfied for the consumers is realized.
Preferably, in the step 3), after the bacterial suspension is put into the reactor, the temperature is kept at 33-35 ℃ and the fermentation is carried out for 5-7d.
By adopting the technical scheme, the fermentation is carried out at the constant temperature of 33-35 ℃, and the activity of the compound bacteria is higher at the specific temperature, so that the fermentation efficiency is higher, the content of active ingredients in the extracted pine-red plum extract is higher, and the effect of resisting allergy and relieving is faster.
Preferably, in the step 3), the ratio of the input mass of the bacterial suspension to the mass of the extracting solution is 1-2:1000.
By adopting the technical scheme, the proportion of the bacterial suspension to the extracting solution is specifically selected, so that the content of special components in the extracting solution after the bacterial suspension is put into is proper, and the compound bacteria can continuously generate special metabolites, thereby better inhibiting the generation of irritant substances, leading the irritation of the pine and red plum extract to be lower, and further better playing the anti-allergy and soothing effects.
Preferably, in the step 4), the heating extraction temperature is 88-90 ℃, and the condensing reflux is carried out for 2-3h.
By adopting the technical scheme, the extraction temperature is specifically selected, so that the extraction effect is better, the process cost is effectively reduced, and the method has higher economic value.
Preferably, in the liquid culture medium, the mass ratio of the solvent to the crabapple juice to the lantern juice to the white melon juice to the sweet potato leaf juice to the monopotassium phosphate to the magnesium sulfate to the calcium chloride to the zinc sulfate to the ferric citrate is 100:3-4:1-2:4-5:2-3:0.2-0.3:0.1-0.2:0.005-0.006:0.3-0.4:0.4-0.5.
By adopting the technical scheme, the liquid culture medium can better improve the activity of the compound bacteria by specifically selecting the proportion of each component in the liquid culture medium, so that the culture efficiency of the compound bacteria is higher, the screening effect is better, the fermentation efficiency of the obtained bacterial suspension on the pine and red plum is higher, the subsequent extraction effect is better, the content of active ingredients is higher, and the anti-allergy relieving effect takes effect faster.
Preferably, in the composite bacteria, the mass ratio of Ma Putao cocci, sea fiber monad and coriolus versicolor is 3.3-3.5:0.7-0.9:1.5-1.7.
By adopting the technical scheme, the mass ratio of staphylococcus equi, sea fiber monad and coriolus versicolor can be specifically selected, so that the cell wall of the red plum can be decomposed more efficiently, the fermentation efficiency is higher, the generation of the stimulating components can be reduced better by the metabolite, the stimulation of the red plum extract can be reduced better, and the anti-allergy and relieving effects of the red plum extract can be exerted better.
Preferably, in the extracting solution, the mass ratio of the solvent to the monopotassium phosphate to the magnesium sulfate to the calcium chloride to the zinc sulfate to the ferric citrate is 100:0.2-0.3:0.1-0.2:0.005-0.006:0.3-0.4:0.4-0.5.
By adopting the technical scheme, the activity of the compound bacteria can be well maintained by specifically selecting the mass proportion of each component in the extracting solution, the effect of fermenting the truffle is better, and the subsequent extracting effect is better.
In a second aspect, the application provides an application of a pine-red plum extract, which adopts the following technical scheme:
The application of the pine and red plum extract is added into skin care products to prepare the skin care products with anti-allergy and soothing effects.
By adopting the technical scheme, the skin care product prepared by adding the pine and red plum extract has the effects of well removing red, relieving itching, calming, relieving pain, local anesthesia, diminishing inflammation and the like, can well play roles in resisting and relieving, has quick response, and can quickly relieve discomfort caused by skin allergy of consumers.
In summary, the application has the following beneficial effects:
1. According to the application, by the mutual coordination of staphylococcus equi, sea fiber monad and coriolus versicolor, the fermentation effect is better, so that active ingredients in cells can be better dissolved in a solvent, the extraction effect of the active ingredients is better, the content of the active ingredients in the extract is higher, and the anti-allergy and soothing effects of the pine-red plum extract are faster.
2. According to the application, preferably, the crabapple juice, the lantern juice, the white melon juice and the sweet potato leaf juice are added into the liquid culture medium, so that the composite bacteria in the bacterial suspension formed by culture have a stronger decomposition function on the cell wall of the red plum blossom, and continuously consume the nutrition components in the extracting solution while decomposing the cell wall of the red plum blossom.
Detailed Description
The present application will be described in further detail with reference to examples.
Example 1
A fermentation method of a pine-red plum extract, comprising the steps of:
step 1), preparing a liquid culture medium:
100kg of a solvent was weighed, 3kg of a crabapple juice, 1kg of a lantern juice, 4kg of a white melon juice, 2kg of a sweet potato leaf juice, 0.2kg of monopotassium phosphate, 0.1kg of magnesium sulfate, 0.005kg of calcium chloride, 0.3kg of zinc sulfate and 0.4kg of ferric citrate were put into the solvent, and the mixture was stirred for 5 minutes at a rotation speed of 120r/min to prepare a liquid culture medium.
Step 2), inoculating a composite bacterium in a liquid culture medium, inoculating 3.3g Ma Putao g cocci, 0.7g sea fiber single-cell and 1.5g coriolus versicolor in each 100kg liquid culture medium, and culturing at the constant temperature of 33 ℃ until the OD value is=1, thus obtaining a bacterial suspension.
Step 3), preparing an extracting solution:
Weighing 100kg of solvent, adding 0.2kg of monopotassium phosphate, 0.1kg of magnesium sulfate, 0.005kg of calcium chloride, 0.3kg of zinc sulfate and 0.4kg of ferric citrate into the solvent, and stirring at a rotating speed of 120r/min for 5min to obtain an extract;
Then, putting the truffle into the extracting solution, putting 100kg of truffle into every 100kg of extracting solution, then putting bacterial suspension into every 100kg of extracting solution, putting 0.1kg of bacterial suspension into every 100kg of extracting solution, stirring for 3min at the rotating speed of 30r/min, and then fermenting for 5d at the constant temperature of 33 ℃.
And 4) continuously stirring at the rotating speed of 60r/min, heating the extracting solution to 88 ℃, condensing and refluxing, and extracting for 2 hours.
Step 5), filtering through 200-mesh filter cloth, and taking filtrate.
And 6) concentrating the filtrate by rotary evaporation at 60 ℃ and 0.01Mpa, concentrating the filtrate into paste, and spraying powder and drying the paste to obtain the pine and red plum extract.
Wherein the solvent is deionized water.
Wherein, the crabapple juice is self-made and the preparation method is as follows:
cleaning fresh fructus Begoniae, breaking wall in a wall breaking machine to obtain fructus Begoniae pulp, press-filtering the fructus Begoniae pulp with a press filter, and collecting filtrate to obtain fructus Begoniae juice.
Wherein, the lantern fruit juice is self-made, and the preparation method is as follows:
cleaning fresh fructus Physalis, breaking wall in a wall breaking machine to obtain fructus Physalis pulp, press-filtering with a press filter, and collecting filtrate to obtain fructus Physalis juice.
Wherein, the white melon juice is self-made, and the preparation method is as follows:
cleaning fresh white melon, breaking wall in a wall breaking machine to obtain white melon pulp, press-filtering the white melon pulp with a press filter, and collecting filtrate to obtain white melon juice.
The sweet potato leaf juice is self-made, and the preparation method comprises the following steps:
Cleaning fresh sweet potato leaves, putting into a wall breaking machine for breaking walls to form sweet potato leaf pulp, press-filtering the sweet potato leaf pulp by a press filter, and taking filtrate to obtain sweet potato leaf juice.
Among them, monopotassium phosphate is commercially available and has CAS number: 7778-77-0.
Wherein, the magnesium sulfate is from commercial market, CAS number: 7487-88-9.
Wherein, the calcium chloride is from commercial market, CAS number: 10043-52-4.
Wherein, zinc sulfate is from commercial market, CAS number: 7746-19-7.
Wherein, the ferric citrate is from commercial market, CAS number: 3522-50-7.
Wherein Ma Putao cocci are freeze-dried powder, purchased in Shanghai preservation biotechnology center, strain number: SHBCC D12479.
Wherein, the sea fiber monad is freeze-dried powder, which is purchased in Shanghai preservation biotechnology center, strain number: SHBCC D57965.
Wherein, the coriolus versicolor is freeze-dried powder, which is purchased in Shanghai preservation biotechnology center, and the strain number is as follows: SHBCC D25122.
Wherein the pine and red plum blossom is freshly picked.
Example 2
A fermentation method of a pine-red plum extract, comprising the steps of:
step 1), preparing a liquid culture medium:
100kg of a solvent was weighed, 3.5kg of a crabapple juice, 1.5kg of a lantern juice, 4.5kg of a white melon juice, 2.5kg of a sweet potato leaf juice, 0.25kg of monopotassium phosphate, 0.15kg of magnesium sulfate, 0.0055kg of calcium chloride, 0.35kg of zinc sulfate and 0.45kg of ferric citrate were put into the solvent, and the mixture was stirred for 5 minutes at a rotation speed of 120r/min to prepare a liquid culture medium.
Step 2), inoculating compound bacteria in a liquid culture medium, inoculating Ma Putao g of cocci, 0.8g of sea fiber single-cell bacteria and 1.6g of coriolus versicolor bacteria in each 100kg of the liquid culture medium, and culturing at the constant temperature of 34 ℃ until the OD value is=1 to obtain bacterial suspension.
Step 3), preparing an extracting solution:
Weighing 100kg of solvent, adding 0.25kg of monopotassium phosphate, 0.15kg of magnesium sulfate, 0.0055kg of calcium chloride, 0.35kg of zinc sulfate and 0.45kg of ferric citrate into the solvent, and stirring for 5min at the rotating speed of 120r/min to obtain an extracting solution;
Then, putting the truffle into the extracting solution, putting 100kg of truffle into every 100kg of extracting solution, then putting bacterial suspension into every 100kg of extracting solution, putting 0.15kg of bacterial suspension into every 100kg of extracting solution, stirring for 3min at the rotating speed of 30r/min, and then fermenting for 6d at the constant temperature of 34 ℃.
And 4) continuously stirring at the rotating speed of 60r/min, heating the extracting solution to 89 ℃, condensing and refluxing, and extracting for 2.5h.
Step 5), filtering through 200-mesh filter cloth, and taking filtrate.
And 6) concentrating the filtrate by rotary evaporation at 60 ℃ and 0.01Mpa, concentrating the filtrate into paste, and spraying powder and drying the paste to obtain the pine and red plum extract.
Wherein the solvent is deionized water.
Wherein, the crabapple juice is self-made and the preparation method is as follows:
cleaning fresh fructus Begoniae, breaking wall in a wall breaking machine to obtain fructus Begoniae pulp, press-filtering the fructus Begoniae pulp with a press filter, and collecting filtrate to obtain fructus Begoniae juice.
Wherein, the lantern fruit juice is self-made, and the preparation method is as follows:
cleaning fresh fructus Physalis, breaking wall in a wall breaking machine to obtain fructus Physalis pulp, press-filtering with a press filter, and collecting filtrate to obtain fructus Physalis juice.
Wherein, the white melon juice is self-made, and the preparation method is as follows:
cleaning fresh white melon, breaking wall in a wall breaking machine to obtain white melon pulp, press-filtering the white melon pulp with a press filter, and collecting filtrate to obtain white melon juice.
The sweet potato leaf juice is self-made, and the preparation method comprises the following steps:
Cleaning fresh sweet potato leaves, putting into a wall breaking machine for breaking walls to form sweet potato leaf pulp, press-filtering the sweet potato leaf pulp by a press filter, and taking filtrate to obtain sweet potato leaf juice.
Among them, monopotassium phosphate is commercially available and has CAS number: 7778-77-0.
Wherein, the magnesium sulfate is from commercial market, CAS number: 7487-88-9.
Wherein, the calcium chloride is from commercial market, CAS number: 10043-52-4.
Wherein, zinc sulfate is from commercial market, CAS number: 7746-19-7.
Wherein, the ferric citrate is from commercial market, CAS number: 3522-50-7.
Wherein Ma Putao cocci are freeze-dried powder, purchased in Shanghai preservation biotechnology center, strain number: SHBCC D12479.
Wherein, the sea fiber monad is freeze-dried powder, which is purchased in Shanghai preservation biotechnology center, strain number: SHBCC D57965.
Wherein, the coriolus versicolor is freeze-dried powder, which is purchased in Shanghai preservation biotechnology center, and the strain number is as follows: SHBCC D25122.
Wherein the pine and red plum blossom is freshly picked.
Example 3
A fermentation method of a pine-red plum extract, comprising the steps of:
step 1), preparing a liquid culture medium:
100kg of a solvent was weighed, 4kg of a crabapple juice, 2kg of a lantern juice, 5kg of a white melon juice, 3kg of a sweet potato leaf juice, 0.3kg of monopotassium phosphate, 0.2kg of magnesium sulfate, 0.006kg of calcium chloride, 0.4kg of zinc sulfate and 0.5kg of ferric citrate were put into the solvent, and the mixture was stirred for 5 minutes at a rotation speed of 120r/min to prepare a liquid culture medium.
Step 2), inoculating compound bacteria in a liquid culture medium, inoculating Ma Putao g of cocci, 0.9g of sea fiber single-cell bacteria and 1.7g of coriolus versicolor in each 100kg of the liquid culture medium, and culturing at the constant temperature of 35 ℃ until the OD value is=1 to obtain bacterial suspension.
Step 3), preparing an extracting solution:
Weighing 100kg of solvent, adding 0.3kg of monopotassium phosphate, 0.2kg of magnesium sulfate, 0.006kg of calcium chloride, 0.4kg of zinc sulfate and 0.5kg of ferric citrate into the solvent, and stirring at a rotating speed of 120r/min for 5min to obtain an extract;
then, putting the truffle into the extracting solution, putting 100kg of truffle into every 100kg of extracting solution, then putting bacterial suspension into every 100kg of extracting solution, putting 0.2kg of bacterial suspension into every 100kg of extracting solution, stirring for 3min at the rotating speed of 30r/min, and then fermenting for 7d at the constant temperature of 35 ℃.
And 4) continuously stirring at the rotating speed of 60r/min, heating the extracting solution to 90 ℃, condensing and refluxing, and extracting for 3 hours.
Step 5), filtering through 200-mesh filter cloth, and taking filtrate.
And 6) concentrating the filtrate by rotary evaporation at 60 ℃ and 0.01Mpa, concentrating the filtrate into paste, and spraying powder and drying the paste to obtain the pine and red plum extract.
Wherein the solvent is deionized water.
Wherein, the crabapple juice is self-made and the preparation method is as follows:
cleaning fresh fructus Begoniae, breaking wall in a wall breaking machine to obtain fructus Begoniae pulp, press-filtering the fructus Begoniae pulp with a press filter, and collecting filtrate to obtain fructus Begoniae juice.
Wherein, the lantern fruit juice is self-made, and the preparation method is as follows:
cleaning fresh fructus Physalis, breaking wall in a wall breaking machine to obtain fructus Physalis pulp, press-filtering with a press filter, and collecting filtrate to obtain fructus Physalis juice.
Wherein, the white melon juice is self-made, and the preparation method is as follows:
cleaning fresh white melon, breaking wall in a wall breaking machine to obtain white melon pulp, press-filtering the white melon pulp with a press filter, and collecting filtrate to obtain white melon juice.
The sweet potato leaf juice is self-made, and the preparation method comprises the following steps:
Cleaning fresh sweet potato leaves, putting into a wall breaking machine for breaking walls to form sweet potato leaf pulp, press-filtering the sweet potato leaf pulp by a press filter, and taking filtrate to obtain sweet potato leaf juice.
Among them, monopotassium phosphate is commercially available and has CAS number: 7778-77-0.
Wherein, the magnesium sulfate is from commercial market, CAS number: 7487-88-9.
Wherein, the calcium chloride is from commercial market, CAS number: 10043-52-4.
Wherein, zinc sulfate is from commercial market, CAS number: 7746-19-7.
Wherein, the ferric citrate is from commercial market, CAS number: 3522-50-7.
Wherein Ma Putao cocci are freeze-dried powder, purchased in Shanghai preservation biotechnology center, strain number: SHBCC D12479.
Wherein, the sea fiber monad is freeze-dried powder, which is purchased in Shanghai preservation biotechnology center, strain number: SHBCC D57965.
Wherein, the coriolus versicolor is freeze-dried powder, which is purchased in Shanghai preservation biotechnology center, and the strain number is as follows: SHBCC D25122.
Wherein the pine and red plum blossom is freshly picked.
Comparative example 1
The extraction method of the pine-red plum extract is different from that of the embodiment 2 only in that:
In the liquid culture medium, the wax apple juice is replaced by the wax apple juice in an equivalent way.
The wax apple juice is self-made and the preparation method is as follows:
Cleaning fresh wax apple, breaking wall in a wall breaking machine to form wax apple pulp, press-filtering the wax apple pulp by a press filter, and collecting filtrate to obtain wax apple juice.
Comparative example 2
The extraction method of the pine-red plum extract is different from that of the embodiment 2 only in that:
in the liquid culture medium, pineapple juice is used for replacing lantern juice in an equivalent way.
The pineapple juice is self-made and the preparation method is as follows:
cleaning fresh pineapple, breaking wall in a wall breaking machine to obtain pineapple pulp, press-filtering the pineapple pulp with a press filter, and collecting filtrate to obtain pineapple juice.
Comparative example 3
The extraction method of the pine-red plum extract is different from that of the embodiment 2 only in that:
in the liquid culture medium, the balsam pear juice is used for replacing the white melon juice in an equivalent way.
The balsam pear juice is self-made and the preparation method is as follows:
Cleaning fresh fructus Momordicae Charantiae, breaking wall in a wall breaking machine to obtain fructus Momordicae Charantiae pulp, press filtering with a press filter, and collecting filtrate to obtain fructus Momordicae Charantiae juice.
Comparative example 4
The extraction method of the pine-red plum extract is different from that of the embodiment 2 only in that:
in the liquid culture medium, spinach juice is used for replacing sweet potato leaf juice in an equivalent way.
The spinach juice is self-made and is prepared by the following steps:
Cleaning fresh spinach, breaking wall in a wall breaking machine to obtain spinach pulp, press-filtering the spinach pulp with a press filter, and collecting filtrate to obtain spinach juice.
Comparative example 5
The extraction method of the pine-red plum extract is different from that of the embodiment 2 only in that:
In the liquid culture medium, the same amount of wax apple juice is adopted to replace the crab apple juice; adopting pineapple juice to replace lantern juice in equal quantity; the balsam pear juice is adopted to replace the white melon juice in equal quantity; the spinach juice is used for replacing the sweet potato leaf juice in an equivalent way.
The wax apple juice is self-made and the preparation method is as follows:
Cleaning fresh wax apple, breaking wall in a wall breaking machine to form wax apple pulp, press-filtering the wax apple pulp by a press filter, and collecting filtrate to obtain wax apple juice.
The pineapple juice is self-made and the preparation method is as follows:
cleaning fresh pineapple, breaking wall in a wall breaking machine to obtain pineapple pulp, press-filtering the pineapple pulp with a press filter, and collecting filtrate to obtain pineapple juice.
The balsam pear juice is self-made and the preparation method is as follows:
Cleaning fresh fructus Momordicae Charantiae, breaking wall in a wall breaking machine to obtain fructus Momordicae Charantiae pulp, press filtering with a press filter, and collecting filtrate to obtain fructus Momordicae Charantiae juice.
The spinach juice is self-made and is prepared by the following steps:
Cleaning fresh spinach, breaking wall in a wall breaking machine to obtain spinach pulp, press-filtering the spinach pulp with a press filter, and collecting filtrate to obtain spinach juice.
Comparative example 6
The extraction method of the pine-red plum extract is different from that of the embodiment 2 only in that:
In the composite bacteria, trichoderma reesei is adopted to replace staphylococcus equi in an equivalent way.
Trichoderma reesei was purchased from Beijing Bai Ou Bo Wei Biotechnology Co., ltd., accession number: ATCC 24449.
Comparative example 7
The extraction method of the pine-red plum extract is different from that of the embodiment 2 only in that:
In the composite bacteria, polysaccharide-dissolving clostridium is adopted to replace sea fiber single-cell bacteria in an equivalent way.
Polysaccharide-dissolving clostridium is purchased from Beijing Bai Ou Bo Wei Biotechnology Co., ltd., accession number: ATCC 33142.
Comparative example 8
The extraction method of the pine-red plum extract is different from that of the embodiment 2 only in that:
In the composite bacteria, vibrio fibrinolyticus is adopted to replace coriolus versicolor in an equivalent way.
Vibrio fibrinolyticus is purchased from beijing Bai-o-Bo-Wei biotechnology Co., ltd., accession number: ATCC 27208.
Comparative example 9
The extraction method of the pine-red plum extract is different from that of the embodiment 2 only in that:
In the composite bacteria, the Trichoderma reesei is adopted to replace Ma Putao cocci in an equivalent way; the polysaccharide dissolving clostridium is adopted to equally replace the sea fiber monad; and replacing Coriolus versicolor with vibrio fibrinolyticus in equal quantity.
Trichoderma reesei was purchased from Beijing Bai Ou Bo Wei Biotechnology Co., ltd., accession number: ATCC 24449.
Polysaccharide-dissolving clostridium is purchased from Beijing Bai Ou Bo Wei Biotechnology Co., ltd., accession number: ATCC 33142.
Vibrio fibrinolyticus is purchased from beijing Bai-o-Bo-Wei biotechnology Co., ltd., accession number: ATCC 27208.
Experiment 1
The Irritation Score (IS) of the test sample prepared from the extract of pine red plum prepared in each example and comparative example was measured according to SNT2329-2009 "cosmetic eye-irritating corrosive chick embryo chorioallantoic membrane test".
The preparation method of the sample to be tested comprises the following steps:
1g of pine and red plum extract and 9g of deionized water are taken and uniformly mixed until the mixture is completely dissolved, and a sample to be tested is obtained.
Experiment 2
Several volunteers were summoned, aged 30-40 years, all women, all had skin allergy, and the total skin loss was between 90-110.
The pine and red plum extracts prepared in each example and comparative example are used by volunteers in groups, and the pine and red plum extracts are firstly cleaned by facial cleanser without anti-inflammatory effect and are diluted by distilled water until the content of the pine and red plum extract is 1% after the water is wiped off before the use, so that repairing liquid is prepared, 5ml of repairing liquid is taken and is uniformly smeared on the face, and the affected parts are ensured to be covered by the repairing liquid.
Before using the pine and red plum extract, the total skin loss is recorded as the initial total skin loss, and then the total skin loss is recorded again when the pine and red plum extract is used for 3d and 7d, namely the total skin loss when the pine and red plum extract is used for 3d and 7 d. The average was calculated for every 10 persons in one group.
The specific experimental data for experiments 1-2 are detailed in Table 1.
TABLE 1
According to the data comparison in table 1, the irritation of the pine-red plum extract prepared in each example is obviously lower than that of each comparative example, and the residual skin loss amount of each example is obviously lower than that of each comparative example when the pine-red plum extract is used for 3d and 7d, therefore, the pine-red plum extract prepared in each example has lower irritation, higher active ingredient content and better anti-allergy and soothing effects, and the repair degree of skin loss is obviously improved.
Comparative examples 1 to 5 damaged the complex system of malus asiatica juice, cape gooseberry juice, white melon juice, sweet potato leaf juice in the liquid medium, resulting in a decrease in the effect of improving the activity of complex bacteria, resulting in a decrease in the fermentation effect and a decrease in the effect of reducing the irritating substances, and thus in a decrease in the anti-allergy and soothing effects of the prepared pine-red plum extract.
Comparative examples 6-9 damaged the complex system of staphylococcus equi, pseudomonas marinus, coriolus versicolor in the complex bacteria, resulting in a decrease in fermentation effect, a decrease in active ingredient content of the pine and red plum extract, and more irritant substances, resulting in a significant decrease in anti-allergy and soothing effects of the prepared pine and red plum extract.
The present embodiment is only for explanation of the present application and is not to be construed as limiting the present application, and modifications to the present embodiment, which may not creatively contribute to the present application as required by those skilled in the art after reading the present specification, are all protected by patent laws within the scope of claims of the present application.
Claims (5)
1. A fermentation method of a pine-red plum extract is characterized in that: the method comprises the following steps:
step 1), preparing a liquid culture medium;
Step 2), inoculating composite bacteria in the liquid culture medium, and culturing until the OD value is more than or equal to 1 to obtain bacterial suspension;
step 3), preparing an extracting solution, soaking the truffle plum blossom in the extracting solution, then adding a bacterial suspension, and fermenting for 5-7d;
Step 4), heating and extracting;
Step 5), filtering to obtain filtrate;
step 6), concentrating and drying the filtrate to obtain a pine and red plum extract;
The liquid culture medium is a compound of a solvent, crab apple juice, lantern fruit juice, white melon juice, sweet potato leaf juice, monopotassium phosphate, magnesium sulfate, calcium chloride, zinc sulfate and ferric citrate;
the compound bacteria are the compound of staphylococcus equi, sea fiber monad and coriolus versicolor;
the extracting solution is a compound of a solvent, potassium dihydrogen phosphate, magnesium sulfate, calcium chloride, zinc sulfate and ferric citrate;
The strain number of the staphylococcus equi is SHBCC D12479, the strain number of the sea fiber monad is SHBCC D57965, and the strain number of the corious versicolor is SHBCC D25122;
In the liquid culture medium, the mass ratio of the solvent to the crabapple juice to the lantern juice to the white melon juice to the sweet potato leaf juice to the monopotassium phosphate to the magnesium sulfate to the calcium chloride to the zinc sulfate to the ferric citrate is 100:3-4:1-2:4-5:2-3:0.2-0.3:0.1-0.2:0.005-0.006:0.3-0.4:0.4-0.5;
In the composite bacteria, the mass ratio of Ma Putao cocci to sea fiber monad to coriolus versicolor is 3.3-3.5:0.7-0.9:1.5-1.7;
In the extracting solution, the mass ratio of the solvent to the monopotassium phosphate to the magnesium sulfate to the calcium chloride to the zinc sulfate to the ferric citrate is 100:0.2-0.3:0.1-0.2:0.005-0.006:0.3-0.4:0.4-0.5.
2. The method for fermenting a pine-red plum extract according to claim 1, wherein: in the step 3), after the bacterial suspension is put into the fermentation tank, the temperature is kept at 33-35 ℃ and the fermentation time is 5-7d.
3. The method for fermenting a pine-red plum extract according to claim 2, wherein: in the step 3), the ratio of the input mass of the bacterial suspension to the mass of the extracting solution is 1-2:1000.
4. The method for fermenting a pine-red plum extract according to claim 1, wherein: in the step 4), the heating extraction temperature is 88-90 ℃, and the condensing reflux is carried out, and the extraction is carried out for 2-3 hours.
5. Use of the extract of prunus maritima according to any one of claims 1-4, characterized in that: can be added into skin care product to prepare skin care product with antiallergic and soothing effects.
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