CN117730762B - Method for promoting growth of submerged plants under stress of flocculant - Google Patents
Method for promoting growth of submerged plants under stress of flocculant Download PDFInfo
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- CN117730762B CN117730762B CN202410182221.9A CN202410182221A CN117730762B CN 117730762 B CN117730762 B CN 117730762B CN 202410182221 A CN202410182221 A CN 202410182221A CN 117730762 B CN117730762 B CN 117730762B
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Abstract
The invention discloses a method for promoting growth of submerged plants under the stress of flocculant, which comprises the following steps: submerged plant seedlings with good growth vigor are subjected to pre-culture in 5-15% Hoagland nutrient solution after trimming and cleaning, seedlings with consistent growth vigor are selected, and seedlings to be planted are obtained after cleaning; preparing sodium nitroprusside pretreatment solution with the concentration of 100-400 mu M by using CaCl 2 solution under the light-shielding condition; completely immersing the seedlings to be planted in the pretreatment liquid under the constant-temperature light-shielding condition, pretreating for 3-5 hours, and cleaning with deionized water for later use; transplanting the pretreated seedlings into a water body containing a flocculating agent. Aiming at the problem that the growth of submerged plants is inhibited due to pollution of flocculating agents in water, the method improves the tolerance of the plants to flocculating agent toxicity, promotes the growth of the plants in the flocculating agent environment, is suitable for restoring and reconstructing the submerged plants in water with different flocculating agent accumulation amounts, and has simple operation, economic ecology and remarkable effect.
Description
Technical Field
The invention relates to the technical field of water ecological restoration, in particular to a method for promoting growth of submerged plants under the stress of flocculating agents.
Background
Eutrophication of water is one of the important problems facing current global water environments. Long-term eutrophication caused by exceeding of nitrogen and phosphorus pollutants in water can cause cyanobacteria bloom, and the ecological function of the water is declined. The restoration of water eutrophication is mainly a physical, chemical and biological method. Among them, one of the chemical restoration methods commonly used in early stage, a large amount of flocculant (such as aluminum salt, ferric salt, polyacrylamide, etc.) is frequently used, and at present, secondary pollution is caused to the environment. Along with the enrichment of the flocculant in the water ecological system, a certain ecological risk is brought, so that the environment is polluted, aquatic animals and plants are damaged, and even human health is poisoned. Therefore, the ecological risk of the flocculating agent in the water ecological system is controlled, and the flocculating agent is one of urgent and necessary tasks for protecting and treating the water environment at present.
Currently, phytoremediation in the bioremediation technology has been widely used as an emerging means of remediation due to its advantages of environmental friendliness, low treatment cost, low energy consumption, and the like. Along with the aggravation of eutrophication of lakes and polluted water bodies, the water ecological system is gradually degraded, so that submerged vegetation which is one of important components in the ecological system plays a key and long-term role in ecological restoration of the eutrophication lakes and the water bodies by recovery and reconstruction. However, pooling of flocculants in the water ecosystem can lead to degradation of submerged plant growth, significantly reducing its repair capacity. Thus, how to increase the resistance of submerged plants to flocculant poisoning is of urgent and important importance in the remediation of water ecosystems.
Sodium nitroprusside is commonly used as an exogenous nitric oxide donor and is periodically applied during the growth of crops and trees to improve the antioxidant capacity, delay the senescence of leaves, enhance the aluminum stress tolerance and promote the growth of plants. Chinese patent CN 113261565B discloses a compound agent for improving the aluminum resistance of crops and application thereof, relates to the technical field of plant planting, solves the problems of serious toxicity and poor aluminum resistance of crops under the stress of aluminum in the prior art, and adopts the technical scheme that: the sodium hydrosulfide and sodium nitroprusside compound comprises a sodium hydrosulfide solution and a sodium nitroprusside solution, wherein the volume ratio of the sodium hydrosulfide solution to the sodium nitroprusside solution is 1:1-4, wherein the concentration of the sodium hydrosulfide solution is 2-300 mu mol/L, and the concentration of the sodium nitroprusside solution is 0.1-1000 mu mol/L. The invention has the advantages of reducing the growth inhibition of the aluminum to the elongation of the crop roots and improving the aluminum resistance of the crops. The invention aims at the toxic action of trivalent free Al 3+ on crops under acidic conditions, and the toxic action mechanism of the trivalent free Al 3+ on the crops is as follows: toxic free Al 3+ accumulates on the plant root tip cell wall, reduces the elasticity and ductility of the cell wall, further inhibits the elongation of root tip cells, and promotes the growth of plant root systems to be inhibited, thereby preventing the plants from absorbing water and nutrients.
In addition, the invention aims at the long-term blade spraying in the aluminum stress process of the sodium nitroprusside-containing compound agent, but the method is easy to cause secondary pollution to the water body, brings certain ecological risk hidden trouble to other aquatic animals, and consumes certain manpower and material resource cost.
The aluminum element has amphiprotic properties and forms which are different under different pH values, and a great deal of researches in the past consider that the toxic effect of aluminum mainly occurs in an acidic environment, and the aluminum is mainly considered to exist in an insoluble Al (OH) 3 stable form under a neutral condition, so that the toxicity is not caused. The pH values of lakes and water bodies in China are generally neutral to slightly alkaline, and with the enrichment of flocculating agents (such as aluminum salt, ferric salt, polyacrylamide and the like) in an aquatic ecosystem, the flocculating agents influence the growth of submerged plants, and aiming at the stress of the flocculating agents in the aquatic ecosystem on the growth of the submerged plants, a method for relieving the poison of the flocculating agents to the submerged plants is developed, which is simple to operate, wide in application range and low in economic cost, and has urgent and important significance in the restoration of the aquatic ecosystem.
Disclosure of Invention
In order to solve one of the problems, the invention provides a method for promoting the growth of submerged plants under the stress of a flocculating agent, which promotes the ecological restoration of the submerged plants, and has the advantages of simple operation, wide application range, low economic cost and remarkable promotion effect.
In order to achieve the above purpose, the technical measures adopted by the invention are as follows:
The invention provides a method for promoting growth of submerged plants under the stress of flocculant, which comprises the following steps:
(1) Pre-culturing seedlings: submerged plant seedlings with good growth vigor are subjected to pruning and cleaning and then are pre-cultured in 5-15% Hoagland nutrient solution for 15-20 days, seedlings with consistent growth vigor are selected, and seedlings to be planted are obtained after cleaning;
(2) Preparing pretreatment liquid: preparing a sodium nitroprusside pretreatment solution with the concentration of 100-400 mu M by using a CaCl 2 solution under a light-shielding condition, and placing the sodium nitroprusside pretreatment solution in a dark place for standby; the high concentration of the sodium nitroprusside pretreatment liquid obviously inhibits the elongation of plant root systems, and the low concentration cannot improve the resistance of submerged plants to flocculant stress;
(3) Seedling pretreatment: completely immersing the seedlings to be planted in the pretreatment liquid under the constant-temperature light-shielding condition, pretreating for 3-5 hours, and then cleaning with deionized water for later use;
(4) Transplanting: transplanting the pretreated seedlings into water containing flocculant and having pH value of 6.5-7.0, and performing ecological restoration of the water.
Further, in the step (2), the CaCl 2 solution concentration is 0.4-0.6 mM.
Further, in the step (3), the constant temperature condition is 24-26 ℃.
Further, the seedlings are trimmed to a height of 10-15 cm in step (1), and the effect of this treatment is that: ensuring the consistency of the initial states of the young seedlings of the bitter grass in different incubators, and more accurately evaluating the poisoning phenomenon of the aluminum salt flocculant on the growth of the bitter grass.
Further, in the step (1), the seedling is washed by deionized water to remove Hoagland nutrient solution attached to the seedling plant. The young grass is typically rinsed 3-4 times with deionized water to thoroughly wash away the Hoagland's nutrient solution adhering to the young plant.
Preferably, in the step (3), the cleaned seedlings are soaked in pure water for standby, and the soaking time is not more than 6 hours.
Further, in the step (4), the flocculant is stressed in the water body, and the flocculant concentration is higher than 2 mg/L.2 mg/L is the critical concentration value of the aluminum salt flocculant for inhibiting the growth of the kuh-seng in the method.
In some embodiments of the application, the flocculant is an aluminum salt flocculant, in particular, a polyaluminum chloride flocculant.
The toxic action mechanism of the flocculating agent in the water body on the aquatic plants is as follows: the flocculant can reduce the concentration of calcium and phosphorus in water, influence the absorption of plant cells to nutrient elements phosphorus and calcium required by growth, and can be chelated with plant growth molecules, thereby inhibiting the growth of submerged plants.
The beneficial effects of the invention are that
Compared with the prior art, the invention has the following beneficial effects: the invention provides a method for promoting growth of broadleaf holly or other submerged plants, aiming at the problem that the growth of broadleaf holly or other submerged plants is inhibited due to pollution of flocculating agents, which is easily caused by chemical reagent restoration in the early stage of water body.
The treated seedling has increased leaf chlorophyll a content and MDA content, shows enhanced tolerance to flocculant toxicity, is suitable for restoring and reconstructing bitter grass or other submerged plants in water bodies with different flocculant accumulation amounts, and has simple operation, economic ecology and remarkable effect.
Compared with the prior art, the method is simple to operate, is suitable for water restoration projects with various pollution degrees from slight to severe, is low in economic cost, does not cause external pollution to the water, improves the survival rate of the sowthistle or other submerged plant seedlings in the planting adaptation period, promotes the growth of the seedlings, and further promotes the reconstruction of the submerged plants so as to improve the stability of an aquatic ecological system and realize water quality purification.
Drawings
FIG. 1 is a schematic view of the structure of an incubator of the present invention;
In the figure: 1. plants; 2. planting a partition plate; 3. a water inlet; 4. a water outlet; 5. an aeration pump; 6. aerated stone;
FIG. 2 is a graph showing comparison of growth vigor observation of seedlings of Sophora flavescens under different preculture conditions in the present invention;
FIG. 3 is a graph showing the effect of different flocculant concentrations on chlorophyll a content in kutsk leaves;
FIG. 4 is a graph showing the effect of different flocculant concentrations on cell death in a grass root system;
FIG. 5 shows a comparison of chlorophyll a content of kutsk leaves under different treatment conditions;
FIG. 6 is a graph showing MDA content comparison of a root system of the herba Sonchi Oleracei under different treatment conditions;
fig. 7 is a graph showing comparison of growth vigor of kucao under different pretreatment durations of sodium nitroprusside solution.
Detailed Description
The following examples are presented herein to demonstrate preferred embodiments of the present invention. It will be appreciated by those skilled in the art that the techniques disclosed in the examples which follow represent techniques discovered by the inventor to function in the practice of the invention, and thus can be considered to constitute preferred modes for its practice. Those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain a like or similar result without departing from the spirit or scope of the invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs, the disclosure of which is incorporated herein by reference as is commonly understood by reference. Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the claims.
The technical scheme of the patent is further described in detail below with reference to the specific embodiments.
A method of promoting growth of flocculant-stressed submerged plants, comprising the steps of:
(1) Pre-culturing seedlings: the method comprises the steps of (1) well growing submerged plant seedlings, pruning the seedlings to 10-15 cm high after cleaning with clear water, then pre-culturing the seedlings in 5-15% Hoagland nutrient solution for 15-20 days, selecting seedlings with consistent growth vigor, and flushing with deionized water to remove Hoagland nutrient solution attached to the seedling plants to obtain seedlings to be planted;
(2) Preparing pretreatment liquid: preparing sodium nitroprusside pretreatment solution with the concentration of 100-400 mu M by using 0.4-0.6 mM CaCl 2 solution under the light-shielding condition, and placing the solution in a dark place for standby;
(3) Seedling pretreatment: completely immersing the seedlings to be planted in the pretreatment liquid at the constant temperature of 24-26 ℃ and in the dark, pretreating for 3-5 hours, and then cleaning with deionized water for later use; the cleaned seedlings can be soaked in pure water for standby, and the soaking time is not more than 6 hours;
(4) Transplanting: transplanting the pretreated seedlings into a water body containing a flocculating agent and having a pH value of 6.5-7.0.
Example 1
The incubator shown in fig. 1 is used, and comprises an incubator body, wherein black light shielding plates are arranged on the periphery of the lower part of the outer side of the incubator body, a water inlet and a water outlet are formed in the incubator body, and aeration is performed to flocculant water body through an aeration pump and aeration stones.
Clear water is added into the incubator body, and the sowthistle seedlings with consistent growth vigor are selected to be planted into the black planting partition plates with the thickness of 10mm in the incubator.
The culture conditions of the incubator are as follows: the temperature of the thermostatic chamber is 25 ℃, the illumination intensity is 3500 lux, and the illumination/darkness time is 12/12 hours.
And (3) supplementing water regularly in the culture process, ensuring the liquid level of the incubator to be consistent, culturing for about 15 days, and collecting the young grass growing under the condition.
Example 2
The incubator shown in fig. 1 is used, and comprises an incubator body, wherein black light shielding plates are arranged on the periphery of the lower part of the outer side of the incubator body, a water inlet and a water outlet are formed in the incubator body, and aeration is performed to flocculant water body through an aeration pump and aeration stones.
Adding clear water into the incubator body, pre-culturing for 15 days with 5% Hoagland nutrient solution, and selecting the seedlings of the Sophora flavescens with consistent growth vigor and clean seedlings of the Sophora flavescens to plant into a black planting partition plate with the thickness of 10mm in the incubator.
The culture conditions of the incubator are as follows: the temperature of the thermostatic chamber is 25 ℃, the illumination intensity is 3500 lux, and the illumination/darkness time is 12/12 hours.
And (3) supplementing water regularly in the culture process, ensuring the liquid level of the incubator to be consistent, culturing for about 15 days, and collecting the young grass growing under the condition.
Example 3
The incubator shown in fig. 1 is used, and comprises an incubator body, wherein black light shielding plates are arranged on the periphery of the lower part of the outer side of the incubator body, a water inlet and a water outlet are formed in the incubator body, and aeration is performed to flocculant water body through an aeration pump and aeration stones.
Adding clear water into the incubator body, pre-culturing for 15 days by using 10% Hoagland nutrient solution, and then selecting the seedlings of the Sophora flavescens with consistent growth vigor and clean seedlings of the Sophora flavescens to plant into a black planting partition plate with the thickness of 10mm in the incubator.
The culture conditions of the incubator are as follows: the temperature of the thermostatic chamber is 25 ℃, the illumination intensity is 3500 lux, and the illumination/darkness time is 12/12 hours.
And (3) supplementing water regularly in the culture process, ensuring the liquid level of the incubator to be consistent, culturing for about 15 days, and collecting the young grass growing under the condition.
Example 4
The incubator shown in fig. 1 is used, and comprises an incubator body, wherein black light shielding plates are arranged on the periphery of the lower part of the outer side of the incubator body, a water inlet and a water outlet are formed in the incubator body, and aeration is performed to flocculant water body through an aeration pump and aeration stones.
Adding clear water into the incubator body, pre-culturing for 15 days with 20% Hoagland nutrient solution, and selecting the seedlings of the Sophora flavescens with consistent growth vigor and clean seedlings of the Sophora flavescens to plant into a black planting partition plate with the thickness of 10mm in the incubator.
The culture conditions of the incubator are as follows: the temperature of the thermostatic chamber is 25 ℃, the illumination intensity is 3500 lux, and the illumination/darkness time is 12/12 hours.
And (3) supplementing water regularly in the culture process, ensuring the liquid level of the incubator to be consistent, culturing for about 15 days, and collecting the young grass growing under the condition.
The growth of seedlings of Sophora flavescens, pre-cultured with clear water, pre-cultured with 5% Hoagland nutrient solution, pre-cultured with 10% Hoagland nutrient solution, and pre-cultured with 20% Hoagland nutrient solution in comparative examples 1 to 4 was observed, and the results are shown in FIG. 2.
The results show that: the leaves of the grass seedlings pre-cultured by clear water turn yellow and grow most slowly, and secondly, 5% of Hoagland nutrient solution and 10% of Hoagland nutrient solution are used for pre-culturing, so that the leaves of the grass plants are greener and grow most vigorously, and when the concentration of the Hoagland nutrient solution exceeds 20%, the leaves of the grass show filiform algae.
Example 5
Adding a polyaluminium chloride flocculant water body with the concentration of 1 mg/L into the incubator body, wherein the pH value of the water body is 7.0. After pre-culturing for 15 days by using 10% Hoagland nutrient solution, selecting the seedlings of the ku grass with consistent growth vigor and cleaning the seedlings of the ku grass to be planted in a black planting partition plate with the thickness of 10mm in an incubator.
The culture conditions of the incubator are as follows: the temperature of the thermostatic chamber is 25 ℃, the illumination intensity is 3500 lux, and the illumination/darkness time is 12/12 hours.
The water is supplemented regularly in the culture process, the liquid level of the incubator is guaranteed to be consistent, the incubator is cultured for about 15 days, and the seedlings of the ku grown in the flocculating agent water body with the concentration of 1 mg/L are collected.
Example 6
Adding a polyaluminium chloride flocculant water body with the concentration of 2 mg/L into the incubator body, wherein the pH value of the water body is 7.0. After pre-culturing for 15 days by using 10% Hoagland nutrient solution, selecting the seedlings of the ku grass with consistent growth vigor and clean seedlings of the ku grass to be planted in a black planting partition plate with the thickness of 10 mm in an incubator.
The culture conditions of the incubator are as follows: the temperature of the thermostatic chamber is 25 ℃, the illumination intensity is 3500 lux, and the illumination/darkness time is 12/12 hours.
The water is supplemented regularly in the culture process, the liquid level of the incubator is guaranteed to be consistent, the incubator is cultured for about 15 days, and the seedlings of the ku grown in the flocculating agent water body with the concentration of 2mg/L are collected.
Example 7
Adding a water body of polyaluminium chloride flocculant with the concentration of 3 mg/L into the incubator body, wherein the pH value of the water body is 7.0. After pre-culturing for 15 days by using 10% Hoagland nutrient solution, selecting the seedlings of the ku grass with consistent growth vigor and clean seedlings of the ku grass to be planted in a black planting partition plate with the thickness of 10 mm in an incubator.
The culture conditions of the incubator are as follows: the temperature of the thermostatic chamber is 25 ℃, the illumination intensity is 3500 lux, and the illumination/darkness time is 12/12 hours. The water is supplemented regularly in the culture process, the liquid level of the incubator is guaranteed to be consistent, the incubator is cultured for about 15 days, and the seedlings of the ku grown in the flocculating agent water body with the concentration of 3 mg/L are collected.
Example 8
Adding a polyaluminium chloride flocculant water body with the concentration of 4 mg/L into the incubator body, wherein the pH value of the water body is 7.0. After pre-culturing in 10% Hoagland nutrient solution for 15 days, selecting young seedlings of Sophora flavescens with consistent growth vigor, and planting into black planting partition plates with thickness of 10 mm in an incubator.
The culture conditions of the incubator are as follows: the temperature of the thermostatic chamber is 25 ℃, the illumination intensity is 3500 lux, and the illumination/darkness time is 12/12 hours. The water is supplemented regularly in the culture process, the liquid level of the incubator is guaranteed to be consistent, the incubator is cultured for about 15 days, and the seedlings of the ku grown in the flocculating agent water body with the concentration of 4 mg/L are collected.
For the young seedlings of Almond obtained in examples 4 to 8, the leaf chlorophyll a content was measured, and the results are shown in Table 1 and FIG. 3; the amount of cell death of the root system was observed, and the results are shown in FIG. 4.
TABLE 1 chlorophyll a content in Sophora Albae leaves cultivated at different flocculant concentrations
The cell death amount of the plant root system is expressed by the Evan's blue staining degree, and the darker the blue color of the root system after staining indicates the larger the cell death amount. As a result, 2mg/L of flocculant can cause cell death of the root system of the broadleaf holly, and the cell death amount of the root system is increased along with the increase of the concentration.
From the above results, it can be seen that the critical concentration value of the flocculant for the growth inhibition of the broadside is 2 mg/L.
Example 9
Adding clear water into the incubator body. After pre-culturing for 15 days by using 10% Hoagland nutrient solution, selecting the seedlings of the ku grass with consistent growth vigor and clean seedlings of the ku grass to be planted in a black planting partition plate with the thickness of 10 mm in an incubator.
The culture conditions of the incubator are as follows: the temperature of the thermostatic chamber is 25 ℃, the illumination intensity is 3500 lux, and the illumination is as follows: the dark time ratio was 12/12 hours.
And water is supplemented regularly in the culture process, the liquid level of the incubator is ensured to be consistent, the incubator is cultured for about 15 days, and the young grass seedlings growing in the water body are collected.
Example 10
Adding CaCl 2 solution with the concentration of 0.5 mM into clear water of a box body of the incubator. After pre-culturing in 10% Hoagland nutrient solution for 15 days, selecting the seedlings of the Sophora flavescens which have consistent growth vigor and are cleaned cleanly, and planting the seedlings into a black planting partition plate with the thickness of 10 mm in an incubator.
The culture conditions of the incubator are as follows: the temperature of the thermostatic chamber is 35 ℃, the illumination intensity is 3500 lux, and the illumination is as follows: the dark time ratio was 12/12 hours.
And water is supplemented regularly in the culture process, the liquid level of the incubator is ensured to be consistent, the incubator is cultured for about 15 days, and the young grass seedlings growing in the water body are collected.
Example 11
Pre-culturing in 10% Hoagland nutrient solution for 15 days at 25deg.C under the condition of keeping away from light, completely immersing the cleaned herba Sonchi Oleracei seedling in 200 μm sodium nitroprusside treatment solution prepared from 0.5 mM CaCl 2 solution, pre-treating for 3 hr, and cleaning with deionized water; soaking cleaned seedling in pure water for no more than 6 hr.
The flocculant water body with the concentration of 2 mg/L is added into the incubator body. The treated ku seedling is planted in a black planting partition plate with the thickness of 10 mm a in an incubator.
The culture conditions of the incubator are as follows: the temperature of the thermostatic chamber is 25 ℃, the illumination intensity is 3500 lux, and the illumination/darkness time is 12/12 hours.
And water is supplemented regularly in the culture process, the liquid level of the incubator is ensured to be consistent, the incubator is cultured for about 15 days, and the young grass seedlings growing in the water body are collected.
Example 12
Pre-culturing in 10% Hoagland nutrient solution for 15 days at 25deg.C under the condition of keeping away from light, completely immersing the cleaned herba Sonchi Oleracei seedling in 200 μm sodium nitroprusside treatment solution prepared from 0.5 mM CaCl 2 solution, pre-treating for 5 hr, and cleaning with deionized water; soaking cleaned seedling in pure water for no more than 6 hr.
The flocculant water body with the concentration of 2 mg/L is added into the incubator body. The treated ku seedling is planted in a black planting partition plate with the thickness of 10 mm a in an incubator.
The culture conditions of the incubator are as follows: the temperature of the thermostatic chamber is 25 ℃, the illumination intensity is 3500 lux, and the illumination/darkness time is 12/12 hours.
And water is supplemented regularly in the culture process, the liquid level of the incubator is ensured to be consistent, the incubator is cultured for about 15 days, and the young grass seedlings growing in the water body are collected.
For the young seedlings of Sophora flavescens obtained in example 6 and example 9 to example 11, the chlorophyll a content and the MDA content of root system in the leaves were measured. The results are shown in Table 2 and FIG. 5, FIG. 6.
TABLE 2 chlorophyll a content and root MDA content in leaves under different treatment conditions
From the results, the CaCl 2 is added into the water body to provide nutrition for plants, and the chlorophyll a content in the leaves of the seedling of the ku with CaCl 2 is higher than that of the ku growing under the condition of clear water; compared with the bitter grass seedlings under the stress of the flocculating agent, after the bitter grass seedlings are pretreated by 200 mu M sodium nitroprusside prepared by 0.5 mM CaCl 2 solution, the chlorophyll a content of leaves of the bitter grass seedlings is increased by 39%, the MDA content of root systems of the bitter grass seedlings is increased by 33% after the bitter grass seedlings are cultured in a water body under the stress of the flocculating agent, and the tolerance to the toxic hazard of the flocculating agent is improved.
The seedlings of Sophora flavescens obtained in example 11 and example 12 were compared with each other, as shown in FIG. 7.
The results show that: under different pretreatment time periods of sodium nitroprusside, the growth conditions of the seedlings of the broadleaf holly are basically consistent, and the great difference is avoided.
All documents mentioned in this disclosure are incorporated by reference in this disclosure as if each were individually incorporated by reference. Further, it will be understood that various changes and modifications may be made by those skilled in the art after reading the above teachings of the application, and such equivalents are intended to fall within the scope of the application as defined by the claims.
Claims (7)
1. A method for promoting growth of a submerged plant under stress of a flocculant, comprising the steps of:
(1) Pre-culturing seedlings: submerged plant seedlings with good growth vigor are subjected to pruning and cleaning and then are pre-cultured in 5-15% Hoagland nutrient solution for 15-20 days, seedlings with consistent growth vigor are selected, and seedlings to be planted are obtained after cleaning;
(2) Preparing pretreatment liquid: preparing a sodium nitroprusside pretreatment solution with the concentration of 100-400 mu M by using a CaCl 2 solution under a light-shielding condition, and placing the sodium nitroprusside pretreatment solution in a dark place for standby;
(3) Seedling pretreatment: completely immersing the seedlings to be planted in the pretreatment liquid under the constant-temperature light-shielding condition, pretreating for 3-5 hours, and then cleaning with deionized water for later use;
(4) Transplanting: transplanting the pretreated seedlings into a water body containing a flocculating agent;
In the step (4), the concentration of the flocculant in the water body containing the flocculant is higher than 2mg/L; the flocculant is an aluminum salt flocculant; the pH value of the water body is 6.5-7.0.
2. The method for promoting the growth of flocculant-stressed submerged plants of claim 1, wherein in step (2), the concentration of CaCl 2 solution is 0.4-0.6mM.
3. The method for promoting the growth of a flocculant-stressed submerged plant of claim 1, wherein in the step (3), the constant temperature condition is 24-26 ℃.
4. The method for promoting the growth of submerged plants under the stress of flocculant according to claim 1, wherein in the step (3), the cleaned seedlings are soaked in pure water for standby, and the soaking time is not more than 6 hours.
5. The method for promoting the growth of flocculant-stressed submerged plants of claim 1, wherein the seedlings in step (1) are trimmed to a height of 10-15 cm.
6. The method of claim 1, wherein the seedling washing in step (1) uses deionized water to wash out the Hoagland's nutrient solution adhering to the seedling plants.
7. A method of promoting the growth of a flocculant stress submerged plant according to claim 1, wherein the flocculant is a polyaluminum chloride flocculant.
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| WO2005108596A1 (en) * | 2004-05-07 | 2005-11-17 | Friedrich-Alexander-Universität Erlangen-Nürnberg | Method for producing cyclic peptides from in vitro plant cell cultures |
| CN112970777A (en) * | 2021-02-19 | 2021-06-18 | 辽宁大学 | Method for strengthening copper pollution resistance of reed by using gas molecule NO |
| CN114455774A (en) * | 2021-12-29 | 2022-05-10 | 河南中汇环保科技有限公司 | Ecological safety flocculant water transparency improving method |
| CN114620838A (en) * | 2022-04-07 | 2022-06-14 | 辽宁大学 | Method for repairing copper-polluted water body by strengthening duckweed |
| CN115784456A (en) * | 2022-12-02 | 2023-03-14 | 江南大学 | Application of sodium nitroprusside in improving purification of eutrophic water body by water hyacinth |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005108596A1 (en) * | 2004-05-07 | 2005-11-17 | Friedrich-Alexander-Universität Erlangen-Nürnberg | Method for producing cyclic peptides from in vitro plant cell cultures |
| CN112970777A (en) * | 2021-02-19 | 2021-06-18 | 辽宁大学 | Method for strengthening copper pollution resistance of reed by using gas molecule NO |
| CN114455774A (en) * | 2021-12-29 | 2022-05-10 | 河南中汇环保科技有限公司 | Ecological safety flocculant water transparency improving method |
| CN114620838A (en) * | 2022-04-07 | 2022-06-14 | 辽宁大学 | Method for repairing copper-polluted water body by strengthening duckweed |
| CN115784456A (en) * | 2022-12-02 | 2023-03-14 | 江南大学 | Application of sodium nitroprusside in improving purification of eutrophic water body by water hyacinth |
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