CN112970777A - Method for strengthening copper pollution resistance of reed by using gas molecule NO - Google Patents
Method for strengthening copper pollution resistance of reed by using gas molecule NO Download PDFInfo
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- CN112970777A CN112970777A CN202110188715.4A CN202110188715A CN112970777A CN 112970777 A CN112970777 A CN 112970777A CN 202110188715 A CN202110188715 A CN 202110188715A CN 112970777 A CN112970777 A CN 112970777A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/24—Cyanogen or compounds thereof, e.g. hydrogen cyanide, cyanic acid, cyanamide, thiocyanic acid
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/327—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae characterised by animals and plants
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2301/00—General aspects of water treatment
- C02F2301/08—Multistage treatments, e.g. repetition of the same process step under different conditions
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Abstract
The invention discloses a method for strengthening copper pollution resistance of reeds by utilizing gas molecules NO. The method comprises the following steps: 1) planting bulrush in the copper polluted water body, adding exogenous NO donor sodium nitroprusside, and performing growth culture; 2) the reed which is grown and cultured is removed from the polluted water body, and is repeatedly planted and repeatedly operated, so that the copper polluted water body is expected to meet the quality standard requirement of II-type water environment of surface water in China. According to the invention, exogenous NO is applied to strengthen the tolerance of the reed to copper pollution, so that the bottlenecks of poor plant tolerance and low absorption efficiency in the past are overcome, the tolerance of the reed to copper is improved, the normal growth of the reed is kept, the water quality can be purified, the concentration of heavy metal copper in a water body environment is obviously reduced, and the heavy metal enriched in a plant body can be recycled, so that the method has good economic and social benefits, and is beneficial to large-scale popularization and application.
Description
Technical Field
The invention relates to the technical field of copper pollution water body tolerance repair, in particular to a method for strengthening copper pollution tolerance of reeds by utilizing gas molecules NO.
Background
At present, with the acceleration of the industrialization process, the discharge amount of heavy metal wastewater is huge, the heavy metal pollution is increasingly serious, and the heavy metal pollution of a water body becomes one of the current main environmental problems. Heavy metals in water mainly come from key pollution industries such as electroplating, mining and metallurgy, electronic manufacturing, thermal power generation and the like. Heavy metal contaminants are relatively stable and not degradable in water and when they accumulate to a certain extent in water they can cause serious damage to the water-aquatic plant-aquatic animal system and may directly or indirectly affect human health through the food chain. Therefore, heavy metal pollution in the water body not only can influence sustainable utilization of water resources, but also can seriously threaten the living environment of aquatic organisms. Researches show that with the rapid development of industries such as metal smelting, mineral mining, animal husbandry and the like in China, a large amount of wastewater and waste residues containing Cu pollutants enter soil and water, and the Cu pollution degree of the soil and the water is increased. Copper (Cu) is a trace element necessary for plant growth and development, participates in a plurality of processes of photosynthesis and the like of plants, plays an irreplaceable role in a plurality of enzymes related to cell metabolism and photosynthesis, but excessive Cu in plant tissues can generate toxic action on the plants, for example, excessive Cu absorption can break nutrient balance, inhibit the absorption and running of nutrient elements, influence processes of an antioxidant system, a cell structure, nutrient element metabolism and the like, inhibit the photosynthesis, reduce the activity of enzyme, and accordingly show visible symptoms, such as plant leaf greening, root elongation obstruction, root blackening, lateral root growth inhibition and the like.
The traditional methods mainly adopt physical and chemical methods for treating the heavy metal pollution of water bodies, and comprise a precipitation method, a high-molecular trapping agent method, a natural zeolite adsorption method, a membrane technology, an activated carbon adsorption process, an ion exchange method and the like. The methods have the advantages of high purification efficiency, short period and the like, but most of the working procedures are too long, the operation is complicated, secondary pollution is easy to generate, and the treatment cost is expensive. Accordingly, more environmentally friendly, economically viable methods have been sought to replace them. Bioremediation is one of the methods, and since the oil spill pollution of the oil tanker with the number of Valdi is successfully treated by using a bioremediation technology in the United states in 1989, the bioremediation method is applied to various pollution treatments by researchers at home and abroad. In recent years, the technology for restoring the water body polluted by the heavy metals by using plants is gradually developed, the technology realizes ecological restoration by using the effects of absorption, enrichment, storage and the like of the plants with excessive accumulation of the heavy metals on the heavy metals, has the advantages of low cost, high efficiency, energy conservation, secondary pollution avoidance, environmental protection and the like, has potential possibility of heavy metal recovery, and is a hotspot of current biological restoration technology research. The aquatic plant has unique superiority in repairing heavy metal polluted water, and has various aquatic plants and larger research space.
Reed is one of the species that many researchers believe is most suitable for constructing artificial wetlands for phytoremediation. Phragmites communis (Phragmitis australis) belongs to the family of Gramineae and Phragmites communis and belongs to perennial rhizome grasses, is widely distributed in wetlands in temperate and tropical areas, and has the advantages of high survival rate, high growth rate, cold resistance, drought resistance, developed root system and the like. At present, the copper pollution is repaired by adopting reed, so that the plant tolerance is poor and the absorption efficiency is low. Therefore, it is of practical significance to adopt some strengthening repair measures to improve the tolerance of the reed to copper pollution.
Disclosure of Invention
The invention aims to provide a method for strengthening the copper pollution resistance of reeds by utilizing gas molecules NO, which can improve the copper resistance of reeds, keep the reeds growing normally, strengthen the copper enrichment of the reeds in a copper-polluted water body, reduce the copper pollution degree in the water body and ensure that the copper-polluted water body can reach the national surface water environment quality standard. The method can realize low-cost, efficient and safer water body remediation and can be practically applied to the remediation of the copper-polluted water body.
In order to achieve the purpose, the invention adopts the technical scheme that the method for strengthening the copper pollution resistance of the reed by utilizing gas molecules NO comprises the following steps:
1) planting bulrush in the copper polluted water body, adding an exogenous NO donor, and performing growth culture;
2) and removing the grown and cultured reeds from the copper-polluted water body, re-planting the reeds, and repeating the operation.
Preferably, in the above method, step 1), the growth culture time is not less than 21 days.
In the method, in the step 1), the reed is planted in the copper-polluted water body, namely, the reed seedlings are planted in the copper-polluted water body.
Preferably, the method adjusts the concentration of copper ions in the copper-polluted water body to be 5-10 mg/L.
Preferably, in the above method, the exogenous NO donor is nitroprusside.
Preferably, in the method, the addition amount of the exogenous NO donor sodium nitroprusside is 0.05-2 mmol/L.
Preferably, in the method, the adding amount of the exogenous NO donor sodium nitroprusside is 0.1 mmol/L.
In the invention, the possible mechanism that NO can relieve heavy metal Cu stress and improve the tolerance of the heavy metal Cu is as follows: firstly, NO can be used as an antioxidant to directly react with active oxygen to eliminate excessive active oxygen in plants, thereby relieving oxidative stress caused by heavy metals. Second, NO participates in the regulation of the antioxidant system, reducing MDA and H2O2Accumulation of (c), etc. Third, NO can activate the expression of resistance genes through a signal cascade amplification process, thereby increasing the resistance of plants.
The invention has the beneficial effects that:
(1) according to the invention, the tolerance of the reed to copper pollution is strengthened by adding exogenous NO, and the reed strengthened by gas molecule NO has an obvious effect on the tolerance of the reed to copper pollution by observing the growth, development and physiological characteristics of the reed.
(2) The method of the invention makes up the limitation of the reed on the basis of consolidating the inherent advantages of the reed by adding the exogenous NO. The normal growth and development of the reeds are ensured while the tolerance of the reeds to copper pollution is improved, so that the growth of the reeds is not influenced by the copper pollution.
(3) The plant selected by the invention avoids the defects of plant restoration such as short plant, slow growth speed, small biomass of overground part and the like.
(4) The invention does not need to introduce foreign plant species and does not need to carry out genetic engineering modification, thereby reducing the invasion or gene pollution of the foreign species.
In a word, the method strengthens the tolerance of the reed to copper pollution by applying gas molecules NO, overcomes the bottlenecks of poor plant tolerance and low absorption efficiency in the past, can purify water quality, obviously reduce the concentration of heavy metal copper in a water body environment, can recycle heavy metals enriched in plant bodies, saves resources, protects the environment, has good economic and social benefits, and is beneficial to large-scale popularization and application.
Nitric Oxide (NO) as a water-soluble and fat-soluble gas small molecule signal substance widely distributed in organisms freely shuttles between the cell water solution part and the lipid phase of a cell membrane, widely participates in various physiological processes of plants, and influences seed germination, growth and development, photosynthesis, root growth, stress resistance reaction and the like. Sodium Nitroprusside (SNP) is a commonly used exogenous NO donor, sodium nitroprusside is dissolved in water to release NO, and 0.5mmol of SNP can generate about 0.2 mu mol of NO. Exogenous SNP is applied, so that the physiological conditions of leaves and roots of pepper plants can be improved, the activity of antioxidant enzyme is improved, and the method is beneficial to being adaptive to the environment. By adding NO from an external source, the physiological condition of the festuca arundinacea under lead stress is improved, the damage of lead to the festuca arundinacea is weakened, the absorption of lead is increased, and the aim of improving the repairing effect of the festuca arundinacea is fulfilled.
Detailed Description
Example 1
A method for strengthening the copper pollution resistance of reed by using gas molecule NO comprises the following steps:
1) fresh reed seedlings are selected and put into water for culturing for 15 days, then reeds with the same growth vigor are selected, 6 reeds are taken as a group and planted in 3000ml beakers containing 2500ml of improved Hoagland nutrient solution for culturing, the beakers are randomly placed and placed in a greenhouse with the same conditions for re-culturing, the nutrient solution is replaced every two days and randomly placed again, and errors caused by environment differences are reduced. Continuously culturing for 7 days, and using the plant in a water culture experiment after the plant grows stably;
2) planting the reed seedlings in a copper-polluted water body with the copper concentration of 5-10mg/L, adding 0.05-0.2mmol/L of exogenous NO donor sodium nitroprusside, and growing and culturing for 21 days;
3) and removing the grown and cultured reeds from the polluted water body, planting the same number of reed seedlings, and repeating the operation for many times until the copper content in the water body is less than 1mg/L, so that the quality standard requirement of the II-class water environment of the surface water in China is met.
(II) detecting the tolerance effect of the gas molecule NO strengthened reed on copper pollution by adopting a water culture experimental method
Nutrient solution is prepared according to different treatment settings for culture, and the nutrient solution is replaced every 2 days. Treatment setting (1) Cu2+5mg/L + SNP 0mmol/L (control); (2) cu2+5mg/L+SN P0.05mmol/L；(3)Cu2+5mg/L+SNP 0.1mmol/L；(4)Cu2+5mg/L+SNP 0.2mmol/L。Cu2+With CuSO4·5H2O (analytically pure) and NO in the form of an aqueous SNP solution. The test vessel was a 3000ml beaker and 3 replicates were used for each treatment. The reed is cultured in nutrient solution containing copper pollution and different SNP treatments for 21 days to be harvested and detected.
After SNP is added at different levels, the growth parameters and heavy metal content of the reed under copper pollution are shown in Table 1.
TABLE 1 growth parameters and heavy metal content of reeds treated differently
Different letters indicate significant differences between the different treatment groups. The significance test adopts single-factor analysis of variance (Duncan test)
As can be seen from the Cu content of different parts of the reed in Table 1, with the addition of gas molecules NO, the copper concentration in the nutritive organ of the reed is firstly reduced and then increased, when the SNP concentration is 0.05mmol/L, the copper concentration in the stem leaf and the root system is respectively 0.98 and 0.95 times of that in the control group, when the SNP concentration is 0.1mmol/L, the copper concentration is respectively 1.1 and 0.98 times of that in the control group, and when the SNP concentration is 0.2mmol/L, the copper concentration is 124 percent and 103 percent higher than that in the control group. Therefore, the application of the gas molecules can reduce the capability of copper ions to permeate through the root system of the reed, so that the tolerance of the reed to copper pollution is improved.
As can be seen from Table 1, although the growth of the overground part of the reed is not obviously promoted by adding the gas molecule NO, the biomass of the reed is increased, the copper tolerance of the reed is improved, and the inhibition effect of copper stress on the reed is effectively relieved; the growth of the reed root system is inhibited when 0.1mmol/L SNP is added, and the addition of 0.1mmol/L SNP can be found by combining the heavy metal content and the growth index, so that the reed can keep a better growth condition while the tolerance to copper stress is improved.
In conclusion, compared with a blank control group, the gas molecule NO reinforced reed group disclosed by the invention can relieve the stress influence of copper stress on the growth of the reed under the condition of copper pollution, and improve the tolerance of the reed to the copper pollution, and the optimal effect is achieved when the addition amount of the SNP is 0.1 mmol/L.
Claims (7)
1. A method for strengthening the tolerance of reed to copper pollution by using gas molecule NO is characterized by comprising the following steps:
1) planting bulrush in the copper polluted water body, adding an exogenous NO donor, and performing growth culture;
2) and removing the grown and cultured reeds from the copper-polluted water body, re-planting the reeds, and repeating the operation.
2. The method according to claim 1, wherein the growth culture time in step 1) is not less than 21 days.
3. The method as claimed in claim 2, wherein the step 1) of planting the reed in the copper-polluted water body is planting a reed seedling in the copper-polluted water body.
4. The method of claim 3, wherein the concentration of copper ions in the copper-contaminated water is adjusted to be 5-10 mg/L.
5. The method of claim 4, wherein the exogenous NO donor is nitroprusside.
6. The method as claimed in claim 5, wherein the amount of the added exogenous NO donor sodium nitroprusside is 0.05-2 mmol/L.
7. The method according to claim 6, wherein the exogenous NO donor, nitroprusside, is added in an amount of 0.1 mmol/L.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114276958A (en) * | 2021-12-20 | 2022-04-05 | 无锡弘焕微生态科技有限公司 | Preparation method and application of triple probiotic fermented compound with anti-inflammatory effect |
| CN114477463A (en) * | 2022-01-28 | 2022-05-13 | 辽宁大学 | Optimization method for restoring copper-polluted water body by scindapsus aureus based on response curved surface |
| CN115784456A (en) * | 2022-12-02 | 2023-03-14 | 江南大学 | Application of sodium nitroprusside in improving purification of eutrophic water body by water hyacinth |
| CN117730762A (en) * | 2024-02-19 | 2024-03-22 | 浙江省生态环境科学设计研究院 | Method for promoting growth of submerged plants under stress of flocculant |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114276958A (en) * | 2021-12-20 | 2022-04-05 | 无锡弘焕微生态科技有限公司 | Preparation method and application of triple probiotic fermented compound with anti-inflammatory effect |
| CN114477463A (en) * | 2022-01-28 | 2022-05-13 | 辽宁大学 | Optimization method for restoring copper-polluted water body by scindapsus aureus based on response curved surface |
| CN115784456A (en) * | 2022-12-02 | 2023-03-14 | 江南大学 | Application of sodium nitroprusside in improving purification of eutrophic water body by water hyacinth |
| CN115784456B (en) * | 2022-12-02 | 2023-08-25 | 江南大学 | Application of sodium nitroprusside in improving purification of eutrophic water body of water hyacinth |
| CN117730762A (en) * | 2024-02-19 | 2024-03-22 | 浙江省生态环境科学设计研究院 | Method for promoting growth of submerged plants under stress of flocculant |
| CN117730762B (en) * | 2024-02-19 | 2024-05-03 | 浙江省生态环境科学设计研究院 | Method for promoting growth of submerged plants under stress of flocculant |
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Application publication date: 20210618 |