CN117717492A - Biological agent for repairing armpit microecology and preparation method and application thereof - Google Patents
Biological agent for repairing armpit microecology and preparation method and application thereof Download PDFInfo
- Publication number
- CN117717492A CN117717492A CN202311752001.7A CN202311752001A CN117717492A CN 117717492 A CN117717492 A CN 117717492A CN 202311752001 A CN202311752001 A CN 202311752001A CN 117717492 A CN117717492 A CN 117717492A
- Authority
- CN
- China
- Prior art keywords
- repairing
- armpit
- biological agent
- acid
- microecology
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003124 biologic agent Substances 0.000 title claims abstract description 68
- 238000002360 preparation method Methods 0.000 title claims abstract description 48
- TXFPEBPIARQUIG-UHFFFAOYSA-N 4'-hydroxyacetophenone Chemical compound CC(=O)C1=CC=C(O)C=C1 TXFPEBPIARQUIG-UHFFFAOYSA-N 0.000 claims abstract description 34
- 108090000988 Lysostaphin Proteins 0.000 claims abstract description 27
- 239000002253 acid Substances 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 25
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 23
- WHMDKBIGKVEYHS-IYEMJOQQSA-L Zinc gluconate Chemical compound [Zn+2].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O WHMDKBIGKVEYHS-IYEMJOQQSA-L 0.000 claims abstract description 19
- 239000011670 zinc gluconate Substances 0.000 claims abstract description 19
- 235000011478 zinc gluconate Nutrition 0.000 claims abstract description 19
- 229960000306 zinc gluconate Drugs 0.000 claims abstract description 19
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 18
- QBYIENPQHBMVBV-HFEGYEGKSA-N (2R)-2-hydroxy-2-phenylacetic acid Chemical compound O[C@@H](C(O)=O)c1ccccc1.O[C@@H](C(O)=O)c1ccccc1 QBYIENPQHBMVBV-HFEGYEGKSA-N 0.000 claims abstract description 16
- IWYDHOAUDWTVEP-UHFFFAOYSA-N R-2-phenyl-2-hydroxyacetic acid Natural products OC(=O)C(O)C1=CC=CC=C1 IWYDHOAUDWTVEP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 229960002510 mandelic acid Drugs 0.000 claims abstract description 16
- 102000016943 Muramidase Human genes 0.000 claims abstract description 15
- 108010014251 Muramidase Proteins 0.000 claims abstract description 15
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims abstract description 15
- 229960000274 lysozyme Drugs 0.000 claims abstract description 15
- 235000010335 lysozyme Nutrition 0.000 claims abstract description 15
- 239000004325 lysozyme Substances 0.000 claims abstract description 15
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims abstract description 14
- 230000002401 inhibitory effect Effects 0.000 claims abstract description 10
- 210000003491 skin Anatomy 0.000 claims abstract description 10
- 239000004310 lactic acid Substances 0.000 claims abstract description 9
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 9
- 230000028327 secretion Effects 0.000 claims abstract description 6
- 206010016936 Folliculitis Diseases 0.000 claims abstract description 4
- 206010020649 Hyperkeratosis Diseases 0.000 claims abstract description 3
- 210000003780 hair follicle Anatomy 0.000 claims abstract description 3
- 210000002374 sebum Anatomy 0.000 claims abstract description 3
- 238000003756 stirring Methods 0.000 claims description 12
- 239000002537 cosmetic Substances 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 7
- 241000191967 Staphylococcus aureus Species 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 6
- 230000001877 deodorizing effect Effects 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 239000008213 purified water Substances 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 5
- 241000191940 Staphylococcus Species 0.000 claims description 4
- 241000191963 Staphylococcus epidermidis Species 0.000 claims description 4
- 239000007921 spray Substances 0.000 claims description 3
- 239000006260 foam Substances 0.000 claims description 2
- 150000007529 inorganic bases Chemical class 0.000 claims description 2
- 150000007522 mineralic acids Chemical class 0.000 claims description 2
- 150000007524 organic acids Chemical class 0.000 claims description 2
- 150000007530 organic bases Chemical class 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 208000035985 Body Odor Diseases 0.000 abstract description 36
- GETQZCLCWQTVFV-UHFFFAOYSA-N trimethylamine Chemical compound CN(C)C GETQZCLCWQTVFV-UHFFFAOYSA-N 0.000 abstract description 13
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract description 12
- NQRYJNQNLNOLGT-UHFFFAOYSA-N Piperidine Chemical compound C1CCNCC1 NQRYJNQNLNOLGT-UHFFFAOYSA-N 0.000 abstract description 12
- 229910021529 ammonia Inorganic materials 0.000 abstract description 6
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 5
- 210000000040 apocrine gland Anatomy 0.000 abstract description 3
- 206010040904 Skin odour abnormal Diseases 0.000 abstract description 2
- 238000004140 cleaning Methods 0.000 abstract 1
- 230000006589 gland dysfunction Effects 0.000 abstract 1
- 206010055000 Bromhidrosis Diseases 0.000 description 34
- 230000000052 comparative effect Effects 0.000 description 19
- 230000000694 effects Effects 0.000 description 19
- 238000012360 testing method Methods 0.000 description 19
- 238000003287 bathing Methods 0.000 description 9
- 206010040880 Skin irritation Diseases 0.000 description 7
- 231100000475 skin irritation Toxicity 0.000 description 7
- 230000036556 skin irritation Effects 0.000 description 7
- 238000005507 spraying Methods 0.000 description 7
- 230000001954 sterilising effect Effects 0.000 description 7
- 238000011156 evaluation Methods 0.000 description 6
- 230000007794 irritation Effects 0.000 description 6
- 230000002829 reductive effect Effects 0.000 description 6
- 239000011734 sodium Substances 0.000 description 6
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- 206010015150 Erythema Diseases 0.000 description 4
- 206010030113 Oedema Diseases 0.000 description 4
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical group [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 4
- 231100000321 erythema Toxicity 0.000 description 4
- 238000009472 formulation Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000001556 precipitation Methods 0.000 description 4
- CDBYLPFSWZWCQE-UHFFFAOYSA-L sodium carbonate Substances [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 4
- 238000010998 test method Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 208000002557 hidradenitis Diseases 0.000 description 3
- 230000036619 pore blockages Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 208000032544 Cicatrix Diseases 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- 241000295644 Staphylococcaceae Species 0.000 description 2
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 description 2
- BDJRBEYXGGNYIS-UHFFFAOYSA-N azelaic acid group Chemical group C(CCCCCCCC(=O)O)(=O)O BDJRBEYXGGNYIS-UHFFFAOYSA-N 0.000 description 2
- 239000012459 cleaning agent Substances 0.000 description 2
- 238000004332 deodorization Methods 0.000 description 2
- 230000004064 dysfunction Effects 0.000 description 2
- 239000004519 grease Substances 0.000 description 2
- 229940124452 immunizing agent Drugs 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 229960000448 lactic acid Drugs 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 231100000344 non-irritating Toxicity 0.000 description 2
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 231100000241 scar Toxicity 0.000 description 2
- 230000037387 scars Effects 0.000 description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 description 2
- 229910000029 sodium carbonate Inorganic materials 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 2
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 2
- 238000004383 yellowing Methods 0.000 description 2
- 239000005635 Caprylic acid (CAS 124-07-2) Substances 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 208000002847 Surgical Wound Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000000172 allergic effect Effects 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 210000000436 anus Anatomy 0.000 description 1
- 210000004883 areola Anatomy 0.000 description 1
- 208000010668 atopic eczema Diseases 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 239000000022 bacteriostatic agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 239000012159 carrier gas Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 210000000613 ear canal Anatomy 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000003325 follicular Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000003780 keratinization Effects 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000873 masking effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 229960002446 octanoic acid Drugs 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical compound OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 description 1
- -1 picric acid trimethylamine salt Chemical class 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 208000020016 psychiatric disease Diseases 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 235000003441 saturated fatty acids Nutrition 0.000 description 1
- 150000004671 saturated fatty acids Chemical class 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 244000005714 skin microbiome Species 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 230000000472 traumatic effect Effects 0.000 description 1
- LADGBHLMCUINGV-UHFFFAOYSA-N tricaprin Chemical compound CCCCCCCCCC(=O)OCC(OC(=O)CCCCCCCCC)COC(=O)CCCCCCCCC LADGBHLMCUINGV-UHFFFAOYSA-N 0.000 description 1
- 210000001364 upper extremity Anatomy 0.000 description 1
- 210000003905 vulva Anatomy 0.000 description 1
- 239000011787 zinc oxide Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention relates to a biological agent for repairing armpit microecology and a preparation method and application thereof, wherein the biological agent comprises, by mass, 0.001-0.5 part of lysostaphin, 0.1-0.5 part of lysozyme, 0.1-0.6 part of an acid agent, 0.1-1 part of p-hydroxyacetophenone, 0.001-0.005 part of zinc gluconate, 0.01-0.5 part of a pH regulator, 0.0001-0.01 part of EDTA-2Na and the balance of water; the acid agent is selected from one or a combination of lactic acid and mandelic acid. The biological agent can effectively remove ammonia, trimethylamine and piperidine, has remarkable bactericidal effect, and can be used for cleaning hair follicles, inhibiting sebum secretion, relieving skin hyperkeratosis, inhibiting folliculitis, relieving or eliminating body odor of apocrine gland dysfunction patients and repairing armpit microecology.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and in particular relates to a biological agent for repairing armpit microecology, a preparation method and application thereof.
Background
The large amount of malodor in human skin is dependent on abnormal secretory function of apocrine glands, which are present in the armpit, areola, anus, vulva and external auditory canal, where bacteria and organic matter contained in the apocrine glands secretions act to produce saturated fatty acids, forming malodor. The common name of bromhidrosis is bromhidrosis, also called sweat odor, is the odor generated by unsaturated fatty acid generated after the apocrine sweat gland is decomposed by bacteria, and is common in young and strong years, so that psychological disorder is generated for patients, and life, work and social contact are affected.
At present, the underarm odor can be thoroughly cured by removing glands through surgical operation, but the surgical wound is large, obvious scars are left, and the activities of the upper limbs are influenced, and a plurality of complications are caused, so that a minimally invasive small incision underarm odor removing method appears, which has hidden scars and good recovery effect, but still belongs to the field of traumatic treatment; in addition, there are problems that the bromhidrosis removing product containing aluminum powder is liable to cause skin diseases such as excessive keratinization, pore blockage, folliculitis and the like, or the balance of skin flora is destroyed by using a powerful bactericide to cause a great amount of multiplication of harmful bacteria and cause strengthening of bromhidrosis or reduction of long-term use effect, and Chinese patent No. 104800099A discloses a skin cleaning agent, the components of which are prepared by using a bacteriostatic agent containing lysostaphin, an immunizing agent such as chitin and a degrading agent containing the immunizing agent of lysozyme and the like, and have the effects of inhibiting the formation of bacterial plaques of skin, reducing bleeding, diminishing inflammation and the like, but the cleaning agent needs to be used for more than 6 months and has insignificant effect. In addition, the problems of poor effect, excessive smell of masking agents and the like exist in the prior art, so that no obvious medicine and product for treating the bromhidrosis exist at present, and the development of a product for treating the bromhidrosis is very necessary for carrying out non-invasive treatment on patients with apocrine sweat gland secretion dysfunction.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a biological agent for repairing armpit microecology, a preparation method and an application method thereof, and provides more effective treatment means and methods for treating apocrine sweat gland secretion dysfunction patients at present.
In the first aspect, the invention provides a biological agent for repairing armpit microecology, which comprises, by weight, 0.001-0.5 part of lysostaphin, 0.2-0.5 part of lysozyme, 0.1-0.6 part of an acid agent, 0.1-1 part of p-hydroxyacetophenone, 0.001-0.005 part of zinc gluconate, 0.01-0.5 part of a pH regulator, 0.0001-0.01 part of EDTA-2Na and 80-100 parts of water.
Further, the acid agent is selected from one or a combination of lactic acid and mandelic acid.
Further, the pH regulator is selected from one or a combination of inorganic base, organic base, inorganic acid and organic acid;
further, the pH regulator is weak base and weak acid;
preferably, the pH regulator is sodium bicarbonate or sodium carbonate, and the pH is regulated to 3-4.
Preferably, the biological agent comprises, by weight, 0.1-0.2 part of lysostaphin, 0.1-0.2 part of lysozyme, 0.3-0.5 part of an acid agent, 0.1-0.2 part of p-hydroxyacetophenone, 0.001 part of zinc gluconate, 0.01 part of sodium bicarbonate or sodium carbonate, 0.002 part of EDTA-2Na and 98.5-99.5 parts of water.
In a second aspect, the invention also provides a preparation method of the biological agent for repairing armpit microecology, which specifically comprises the following preparation steps:
s1, adding lysostaphin, lysozyme, an acid agent and zinc gluconate into purified water, and uniformly stirring;
s2, adding p-hydroxyacetophenone into hot water at 60-80 ℃, completely dissolving, adding EDTA-2Na, and cooling to 30-40 ℃ for standby;
and S3, mixing the S1 and the S2, stirring, and finally adding a pH regulator to regulate the pH value to 3-4 to obtain the biological preparation for repairing the armpit microecology.
In a third aspect, the invention also provides the use of the biological agent for repairing armpit microecology in cosmetics;
further, the cosmetic may be used to inhibit staphylococci including, but not limited to, staphylococcus epidermidis, staphylococcus aureus.
Further, the cosmetic may also be used for, but not limited to, cleansing hair follicles, inhibiting sebum secretion, alleviating skin overstrain
Hyperkeratosis, inhibition of folliculitis, deodorization and body odor removal.
Further, the cosmetic includes a liquid, a solid, a gel, a foam, or a spray.
The lysostaphin added in the biological preparation for repairing armpit microecology has specific bactericidal property on staphylococci and has certain characteristic of decomposing grease, but has weaker bactericidal capability, if better effect needs to be achieved, the addition amount needs to be increased, the stability is poorer, the degradation is quicker, the addition amount is too high, and a stable preparation is more difficult to form, therefore, the acid agent is added in the compound lysostaphin preparation, the pore can be softened by the mandelic acid with stronger acidity, the penetration of the lysostaphin can be promoted, the grease decomposition capability is improved, the bactericidal effect is improved, and meanwhile, the odor of lactic acid and mandelic acid on ammonia, trimethylammonium, piperidine and the like generated by pore blockage has stronger deodorizing effect, but has stronger irritation on skin.
In order to stabilize the lysostaphin for a long period, the biological preparation for repairing armpit microecology is further added with a trace amount of zinc gluconate to further strengthen the lysostaphin, so that the lysostaphin can keep stable performance in long-term storage and can not cause the lysostaphin to generate precipitation.
Compared with the prior art, the invention has the following beneficial effects:
the biological agent for repairing armpit microecology is mainly compounded by adopting lysostaphin, an acid agent, p-hydroxyacetophenone and zinc gluconate in different proportions, so that the effects of effectively clearing pore blockage and reducing follicular inflammation are achieved, specific proteins are used for killing staphylococcus, the sterilization rate of staphylococcus epidermidis and staphylococcus aureus can reach 99.99%, the staphylococcus is used for decomposing volatile unsaturated fatty acid without raw materials, and the removal effect of substances such as ammonia, trimethylamine and piperidine generated is obvious, thereby repairing armpit microecology and achieving the purpose of radically treating or reducing bromhidrosis and odor.
Detailed Description
The experimental methods of the present invention, in which specific conditions are not specified in the following examples, are generally conducted under conventional conditions or under conditions recommended by the manufacturer. The various chemicals commonly used in the examples are commercially available.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
The present invention will be further described in detail with reference to the following embodiments, in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the description is only illustrative and is not intended to limit the scope of the invention. In addition, in the following description, descriptions of well-known structures and techniques are omitted so as not to unnecessarily obscure the present invention.
The lysostaphin in the biological preparation component for repairing armpit microecology is purchased from biological engineering (Shanghai) Co., ltd (model A619001), and the lysostaphin is purchased from biological engineering (Shanghai) Co., ltd (model A610308).
Example 1
The formulation of the biological agent for repairing armpit microecology prepared in this example is as follows:
the biological preparation for repairing armpit microecology comprises the following components: 0.1 part of lysostaphin, 0.2 part of lysozyme, 0.5 part of mandelic acid, 0.2 part of p-hydroxyacetophenone, 0.001 part of zinc gluconate and Na 2 CO 3 0.01 part of EDTA-2Na 0.002 part and water 98.987 parts.
The preparation is carried out according to the biological agent formula, and the specific preparation steps are as follows:
s1, adding lysostaphin, lysozyme, mandelic acid and zinc gluconate into purified water, and stirring for 15 minutes;
s2, adding the p-hydroxyacetophenone into hot water at 80 ℃, adding EDTA-2Na after complete dissolution, cooling for standby,
s3, mixing the S1 and the S2, stirring for 15 minutes, and finally adding Na 2 CO 3 And adjusting the pH value to 3-4.
Example 2
The formulation of the biological agent for repairing armpit microecology prepared in this example is as follows:
the biological preparation for repairing armpit microecology comprises the following components: lysostaphin 0.2 parts, lysozyme0.1 part of mandelic acid 0.3 part, p-hydroxyacetophenone 0.1 part, zinc gluconate 0.001 part and Na 2 CO 3 0.01 part of EDTA-2Na 0.002 part and water 99.287 parts.
The preparation is carried out according to the biological agent formula, and the specific preparation steps are as follows:
s1, adding lysostaphin, lysozyme, mandelic acid and zinc gluconate into purified water, and uniformly stirring;
s2, adding p-hydroxyacetophenone into hot water at 80 ℃, completely dissolving, adding EDTA-2Na, and cooling for standby;
s3, mixing the S1 and the S2, uniformly stirring, and finally adding Na 2 CO 3 And adjusting the pH value to 3-4.
Example 3
The formulation of the biological agent for repairing armpit microecology prepared in this example is as follows:
the biological preparation for repairing armpit microecology comprises the following components: the enzyme preparation comprises the following components: 0.2 part of lysostaphin, 0.1 part of lysozyme, 0.4 part of lactic acid, 0.1 part of mandelic acid, 0.1 part of p-hydroxyacetophenone, 0.001 part of zinc gluconate and NaHCO 3 0.01 part of EDTA-2Na 0.002 part and water 99.087 parts.
The preparation is carried out according to the biological agent formula, and the specific preparation steps are as follows:
s1, adding lysostaphin, lysozyme, lactic acid, mandelic acid and zinc gluconate into purified water, and uniformly stirring;
s2, adding p-hydroxyacetophenone into hot water at 80 ℃, completely dissolving, adding EDTA-2Na, and cooling for standby;
s3, mixing the S1 and the S2, uniformly stirring, and finally adding NaHCO 3 And adjusting the pH value to 3-4.
Example 4
The formulation of the biological agent for repairing armpit microecology prepared in this example is as follows:
the biological preparation for repairing armpit microecology comprises the following components: the enzyme preparation comprises the following components: 0.2 part of lysostaphin, 0.1 part of lysozyme, 0.2 part of lactic acid, 0.2 part of mandelic acid, 0.1 part of p-hydroxyacetophenone, 0.001 part of zinc gluconate and Na 2 CO 3 0.01 partEDTA-2Na 0.002 parts, water 99.187 parts.
The preparation is carried out according to the biological agent formula, and the specific preparation steps are as follows:
s1, adding lysostaphin, lysozyme, lactic acid, mandelic acid and zinc gluconate into purified water, and uniformly stirring;
s2, adding p-hydroxyacetophenone into hot water at 80 ℃, completely dissolving, adding EDTA-2Na, and cooling for standby;
s3, mixing the S1 and the S2, uniformly stirring, and finally adding Na 2 CO 3 And adjusting the pH value to 3-4.
Comparative example 1
The difference from the best example 4 is that no acid agent is added into the components of the biological agent for repairing armpit microecology, water is adopted to complement the quality, and the rest components and the preparation method are the same as those of the example 4.
Comparative example 2
The difference from the best example 4 is that the component of the biological agent for repairing armpit microecology contains 2 parts of mandelic acid and 97.587 parts of water, and the rest components and the preparation method are the same as those of example 4.
Comparative example 3
The difference from the best example 4 is that the acid agent in the component of the biological agent for repairing armpit microecology is azelaic acid 0.2 parts, mandelic acid 0.2 parts, water 98.187 parts, and the rest components and the preparation method are the same as in example 4.
Comparative example 4
The difference from the best example 4 is that the zinc gluconate in the components of the biological agent for repairing armpit microecology is replaced by nano zinc oxide, and the rest components and the preparation method are the same as those of the example 4.
Comparative example 5
The difference from the best example 4 is that the component of the biological agent for repairing armpit microecology contains 0.01 part of zinc gluconate, and the rest components and the preparation method are the same as those of the example 4.
Comparative example 6
The difference from the best example 4 is that the hydroxyacetophenone in the component of the biological agent for repairing armpit microecology adopts caprylic acid/capric acid triglyceride, and the rest components and the preparation method are the same as those of the example 4.
Comparative example 7
The difference from the preferred example 4 is that the pH of the biological agent for restoring the armpit microecology is 5.5, na 2 CO 3 The addition amount was 0.03 part by weight of water 99.167, and the other components and the production method were the same as those in example 4.
Test example one, in vitro deodorization and sterilization effect evaluation of biological agent for repairing armpit microecology
Test sample: sample of composite lysostaphin preparation prepared in examples 1-4 and comparative examples 1-7;
the test method comprises the following steps: (1) ammonia removal rate was measured according to GB/T33610.2-2017; (2) trimethylamine removal rate: extracting a sample by using anhydrous toluene, reacting trimethylamine with picric acid to generate yellow picric acid trimethylamine salt, measuring absorbance of an extraction solution at 410nm, and quantitatively analyzing by using a standard curve method; (3) piperidine removal rate: detecting with a gas chromatograph by using a SE-54 capillary column (15 mx0.20 mmx0.25m), heating the column temperature by adopting a program, keeping the initial temperature at 70 ℃, keeping the temperature at 5min, keeping the temperature at 10 ℃/min to 100 ℃, keeping the temperature at 2min, keeping the temperature at 25 ℃/min to 200 ℃, keeping the temperature at 5min, keeping the carrier gas at nitrogen at a flow rate of 1.0mL/min, keeping the temperature of a detector and a sample inlet at 260 ℃ for 20min, and keeping the balance time at 20min; (4) the microorganism was measured in accordance with GB/T15979-2002.
The ammonia removal rate, trimethylamine removal rate, piperidine removal rate and sterilization rate were tested by the above test methods, and the results are shown in table 1;
table 1: in vitro test result of biological agent for repairing armpit microecology
As shown in Table 1, the ammonia removal rate of the 3 groups of biological preparations for repairing armpit micro-ecology prepared in examples 1-4 can reach 92.5-96.8%, the trimethylamine removal rate can reach 94.6-98.6%, the piperidine removal rate can reach 90.7-92.8%, and the staphylococcus aureus and staphylococcus epidermidis sterilization rates can reach 99.99%, which indicates that the biological preparation for repairing armpit micro-ecology prepared in examples 1-4 has remarkable inhibiting and sterilizing effects on bromhidrosis and armpit micro-ecology, the biological preparation for repairing armpit micro-ecology prepared in comparative examples 1-7 has remarkable inhibiting effects on the one hand, the results of comparative examples 1-3 show that the influence of an acid agent and a pH value on the deodorizing and sterilizing effects of the biological preparation is large, and meanwhile, the deodorizing effects of mandelic acid and lactic acid on the enzyme preparation are very good, and meanwhile, staphylococcus aureus and staphylococcus aureus are inhibited to a certain extent, but the effects of deodorizing and bacteriostasis of the epidermis are not further improved, and skin irritation is that the acid agent mainly has the effect of inhibiting odor and sterilizing effect on skin, and the effect is that the acid agent is reduced in an excessive amount, and the activity of the acid agent is reduced, and the activity of the acid is partially or the pH value is reduced, and the activity of the acid is not excessively and the activity of the acid agent is reduced, and the activity is partially is reduced, on the basis of the alkaline.
Test example two, evaluation of stability of biological agent for repairing armpit microecology
The biological agent samples prepared in examples 1-4 and comparative examples 1-7 for repairing armpit microecology are stored for 24 months in a cool and dry environment, and the biological agent samples in examples 1-4, comparative examples 1-3 and comparative example 7 are colorless and transparent, and have no phenomena of yellowing, precipitation and the like, so that the biological agent is stable, while the biological agent is precipitated after being stored for 3 months in comparative example 4, a small amount of precipitation occurs in comparative example 5 after being stored for 9 months, a small amount of precipitation occurs in comparative example 6 after being stored for 7 months, and the liquid is yellowing.
Test example three, evaluation of skin irritation of biological agent for repairing armpit microecology
Test sample: biological agent preparation samples for repairing armpit microecology prepared in examples 1-4 and comparative examples 1-7;
the test method comprises the following steps: the complete skin irritation test was performed several times according to the disinfection technical Specification (2002 edition) 2.3.3 and scoring was performed according to the scoring criteria for skin irritation response in the Specification (tables 2-11), and the specific results are shown in Table 2.
Table 2: evaluation result of biological agent body skin irritation for repairing armpit microecology
As shown in Table 2, the biological agent for repairing armpit microecology prepared in examples 1-4 of the present invention has no obvious skin irritation, while comparative example 2 has obvious allergic phenomenon due to excessive addition of the acid agent and large skin irritation, and the visible erythema appears on day 3 of application, obvious erythema appears on day 7 with visible edema, serious erythema appears on day 10, and skin rising and well-defined edema appears, edema 11 is still aggravated, thus the addition of the acid agent needs a proper amount, in addition, comparative example 6 also has visible erythema on day 7, edema appears on day 11 again, and the red spot is aggravated, so that the use irritation of the biological agent for repairing armpit microecology of the present invention can be obviously reduced by the hydroxyacetophenone component, and the mild and non-irritating in the use process can be ensured.
Test example IV, evaluation of human body use effect of biological agent for repairing armpit microecology
Test sample: biological agent samples (experimental groups) for repairing armpit microecology, which are prepared in examples 1-4;
the test method comprises the following steps: 10 volunteers (5 men and 5 women) with bromhidrosis/armpit odor are selected, biological agent samples for repairing armpit microecology are sprayed to the armpit in the morning and after bath for about 3-5 times (about 100 mg), the liquid is contacted with the skin, and the liquid is continuously used for 7-10 days, so that bromhidrosis/armpit odor reduction conditions are recorded;
the evaluation method comprises the following steps:
test object 1: the biological agent sample for repairing armpit microecology prepared in the example 1 is used for spraying the biological agent sample on armpit after bathing in the morning and evening in the case of men, 35 years old, the using amount is about 100mg, the armpit odor is obviously relieved after 7 days of use, the biological agent sample is mild and has no irritation, and the biological agent sample for repairing armpit microecology can be kept for 4-8 hours without obvious armpit odor or armpit odor after spraying.
Test object 2: the biological agent sample for repairing armpit microecology prepared in the example 1 is used for spraying the biological agent sample on armpit after bathing in the morning and evening in the daily use of about 100mg, the armpit odor is obviously relieved after using for 9 days, the biological agent sample is mild and has no irritation, and the biological agent sample for repairing armpit microecology can be kept for 4-8 hours without obvious armpit odor.
Test object 3: the biological agent sample for repairing armpit microecology prepared in example 2 was used for treating male, 45 years old, bromhidrosis/armpit odor by spraying on armpit after bathing in the morning and evening, the usage amount was about 10mg, and bromhidrosis/armpit odor completely disappeared after 10 days of use.
Test object 4: the biological agent sample for repairing armpit microecology prepared in the example 3 is used for spraying the biological agent sample on armpit after bathing in the morning and evening in the case of 30 years old, the using amount is about 100mg, the armpit odor is obviously relieved after 7 days of use, the biological agent sample is mild and has no irritation, and the biological agent sample for repairing armpit microecology can be kept for 4-8 hours without obvious armpit odor.
Test object 5: the biological agent sample for repairing armpit microecology prepared in the example 3 is used for spraying the biological agent sample on armpit after bathing in the morning and evening in the use amount of about 100mg, the armpit odor is obviously relieved after 8 days of use, the biological agent sample is mild and has no irritation, and the biological agent sample for repairing armpit microecology can be kept to have no obvious armpit odor or armpit odor for 4-8 hours after spraying.
Test object 6: women, 26 years old, had bromhidrosis/bromhidrosis, and the armpit microecological biological preparation sample prepared in example 4 was sprayed in the morning and evening after bathing, at an amount of about 10mg, and after 7 days of use, bromhidrosis/bromhidrosis completely disappeared.
Test object 7: women, 23 years old, had bromhidrosis/bromhidrosis, and the armpit microecological biological preparation sample prepared in example 2 was sprayed in the morning and evening after bathing, and the amount of the preparation was about 100mg, and after 10 days of use, bromhidrosis/bromhidrosis was completely disappeared.
Test object 8: women, 30 years old, had bromhidrosis/bromhidrosis, and the armpit microecological biological preparation sample prepared in example 1 was sprayed in the morning and evening after bathing, and the amount of the preparation was about 100mg, and after 10 days of use, bromhidrosis/bromhidrosis was completely disappeared.
Test object 9: women, 35 years old, have bromhidrosis/bromhidrosis, use the biological agent sample of restoration armpit microecology that example 3 prepared, spray to use after the morning and evening bath, use about 100mg, use 8 days later bromhidrosis/bromhidrosis and obviously lighten, and mild and nonirritating feel, can keep 4-8 hours after the biological agent sample of restoration armpit microecology is sprayed and have obvious bromhidrosis/bromhidrosis smell.
Test object 10: women, 48 years old, had bromhidrosis/bromhidrosis, and the armpit microecological biological preparation sample prepared in example 4 was sprayed in the morning and evening after bathing, the usage amount was about 100mg, and bromhidrosis/bromhidrosis completely disappeared after 9 days of use.
It is apparent that the above examples are given by way of illustration only and are not limiting of the embodiments. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. While still being apparent from variations or modifications that may be made by those skilled in the art are within the scope of the invention.
Claims (10)
1. The biological agent for repairing armpit microecology is characterized by comprising, by weight, 0.001-0.5 part of lysostaphin, 0.1-0.5 part of lysozyme, 0.1-0.6 part of an acid agent, 0.1-1 part of p-hydroxyacetophenone, 0.001-0.005 part of zinc gluconate, 0.01-0.5 part of a pH regulator, 0.0001-0.01 part of EDTA-2Na and 80-100 parts of water;
the acid agent is selected from one or a combination of lactic acid and mandelic acid.
2. The biological agent for repairing axillary micro-ecology according to claim 1, wherein the pH adjustor is selected from one or a combination of inorganic base, organic base, inorganic acid, organic acid.
3. The biological agent for repairing axillary micro-ecology according to claim 2, wherein the pH adjustor is weak base and weak acid.
4. The biological agent for repairing armpit microecology according to claim 1, wherein the biological agent comprises, by weight, 0.1-0.2 part of lysostaphin, 0.1-0.2 part of lysozyme, 0.3-0.5 part of an acid agent, 0.1-0.2 part of p-hydroxyacetophenone, 0.001 part of zinc gluconate, 0.01 part of a pH regulator, 0.002 part of EDTA-2Na and 98.5-99.5 parts of water.
5. A method for preparing the biological agent for repairing axillary micro-ecology according to any one of claims 1 to 4, comprising the following preparation steps:
s1, adding lysostaphin, lysozyme, an acid agent and zinc gluconate into purified water, and uniformly stirring;
s2, adding p-hydroxyacetophenone into hot water at 60-80 ℃, completely dissolving, adding EDTA-2Na, and cooling to 30-40 ℃ for standby;
and S3, mixing the S1 and the S2, stirring, and finally adding a pH regulator to regulate the pH value to 3-4 to obtain the biological preparation for repairing the armpit microecology.
6. The use of the biological agent for repairing axillary micro-ecology according to any one of claims 1 to 4 in cosmetics.
7. The use according to claim 6, wherein the cosmetic is for inhibiting staphylococcus spp.
8. The use according to claim 7, wherein said staphylococcus genus comprises staphylococcus epidermidis, staphylococcus aureus.
9. The use according to claim 6, wherein said cosmetic is used for cleansing hair follicles, inhibiting sebum secretion, reducing skin hyperkeratosis, inhibiting folliculitis, deodorizing and deodorizing.
10. The use according to claim 9, wherein the cosmetic comprises a liquid, a solid, a gel, a foam or a spray.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311752001.7A CN117717492A (en) | 2023-12-19 | 2023-12-19 | Biological agent for repairing armpit microecology and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311752001.7A CN117717492A (en) | 2023-12-19 | 2023-12-19 | Biological agent for repairing armpit microecology and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117717492A true CN117717492A (en) | 2024-03-19 |
Family
ID=90210331
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311752001.7A Pending CN117717492A (en) | 2023-12-19 | 2023-12-19 | Biological agent for repairing armpit microecology and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117717492A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1367018A (en) * | 2002-01-28 | 2002-09-04 | 上海高科生物工程有限公司 | Compound preparation of staphylococcolysis enzyme and its preparation method and application |
| CN1438032A (en) * | 2002-01-28 | 2003-08-27 | 上海高科生物工程有限公司 | Compound preparation for dissolving staphyloase and preparation method |
| WO2015085998A1 (en) * | 2013-12-11 | 2015-06-18 | Henkel Ag & Co. Kgaa | Antiperspirant cosmetic agents without compounds containing aluminum |
| CN109289042A (en) * | 2018-10-17 | 2019-02-01 | 李文宜 | A kind of deodorant liquid and its preparation method and application |
| CN110124042A (en) * | 2019-05-28 | 2019-08-16 | 赵叶莲 | The composition of inhibition underarm odor without aluminium |
| CN111093611A (en) * | 2017-07-20 | 2020-05-01 | 托马斯·莱布兰德 | Brevibacillin and preparation thereof for use in treating or preventing body odor |
| US20210009922A1 (en) * | 2019-07-10 | 2021-01-14 | Shannon E Klingman | Methods and compositions for reducing persistent odor in clothing and mitigating biofilms |
| US20220296499A1 (en) * | 2021-03-19 | 2022-09-22 | Eligo Bioscience | Cosmetic use of engineered postbiotics comprising bacteriocins and/or endolysins |
-
2023
- 2023-12-19 CN CN202311752001.7A patent/CN117717492A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1367018A (en) * | 2002-01-28 | 2002-09-04 | 上海高科生物工程有限公司 | Compound preparation of staphylococcolysis enzyme and its preparation method and application |
| CN1438032A (en) * | 2002-01-28 | 2003-08-27 | 上海高科生物工程有限公司 | Compound preparation for dissolving staphyloase and preparation method |
| WO2015085998A1 (en) * | 2013-12-11 | 2015-06-18 | Henkel Ag & Co. Kgaa | Antiperspirant cosmetic agents without compounds containing aluminum |
| CN111093611A (en) * | 2017-07-20 | 2020-05-01 | 托马斯·莱布兰德 | Brevibacillin and preparation thereof for use in treating or preventing body odor |
| CN109289042A (en) * | 2018-10-17 | 2019-02-01 | 李文宜 | A kind of deodorant liquid and its preparation method and application |
| CN110124042A (en) * | 2019-05-28 | 2019-08-16 | 赵叶莲 | The composition of inhibition underarm odor without aluminium |
| US20210009922A1 (en) * | 2019-07-10 | 2021-01-14 | Shannon E Klingman | Methods and compositions for reducing persistent odor in clothing and mitigating biofilms |
| US20220296499A1 (en) * | 2021-03-19 | 2022-09-22 | Eligo Bioscience | Cosmetic use of engineered postbiotics comprising bacteriocins and/or endolysins |
Non-Patent Citations (1)
| Title |
|---|
| 熊德鑫;: "皮肤菌群的微生态学分析和应用展望", 中国微生态学杂志, no. 02, 2 July 1991 (1991-07-02), pages 74 - 81 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US6426092B1 (en) | Powder compositions comprising a skin irritation reducing agent | |
| EP0650720A1 (en) | Skin care and deodorant compositions | |
| CN111110577A (en) | Mild mite-removing itching-relieving shower gel and preparation method thereof | |
| CN108685769A (en) | A kind of herbal mixture black hair anticreep shampoo and preparation method thereof | |
| JPH05501881A (en) | Derivatives of aromatic benzoates as inhibitors of esterase-producing microorganisms | |
| CA2882392A1 (en) | Antiseptic, antiseborrheic and exfoliating composition to remove or prevent acne | |
| GB1561475A (en) | Lastic acid composition | |
| JPH07138179A (en) | Cell activator containing extract from belamcanda chinensis dc. and its application | |
| CN103478177A (en) | Air disinfectant | |
| CN117717492A (en) | Biological agent for repairing armpit microecology and preparation method and application thereof | |
| JP2008184439A (en) | External preparation for skin for ameliorating cytotoxicity of active oxygen species | |
| CN107955756B (en) | Handmade soap and preparation method thereof | |
| CN112057400B (en) | Antiperspirant and preparation method thereof | |
| CN109195592A (en) | For keeping the composition, suite of tools and method of eyelid hygiene | |
| CN110946159A (en) | Plant essence biological compound clothing mildew sweat odor removing agent | |
| CN114642602A (en) | Composition containing colloidal sulfur and application thereof | |
| CN113398050A (en) | Mite-clearing and oil-controlling shower gel | |
| JPH02193919A (en) | Sebaceous gland suppressor | |
| KR20100117261A (en) | Composite of shampoo | |
| CN111631997B (en) | Composition containing compound extracts of kochia scoparia and cnidium fruit and application thereof | |
| CN107616953A (en) | A kind of anti-acne moisturizing skin care item of the cell extract containing banana figs and preparation method thereof | |
| CN113413331B (en) | Acne-removing composition and preparation and application thereof | |
| JPH07187985A (en) | Skin-beautifying agent consisting essentially of phytic acid or its salts | |
| CN109852497B (en) | Traditional Chinese medicine antibacterial soap and preparation method thereof | |
| KR100356697B1 (en) | A skin-composition for pimples therapeutics |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |