CN117701362B - Equipment for extracting nisin from lactococcus lactis fermentation liquor - Google Patents
Equipment for extracting nisin from lactococcus lactis fermentation liquor Download PDFInfo
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- CN117701362B CN117701362B CN202410135992.2A CN202410135992A CN117701362B CN 117701362 B CN117701362 B CN 117701362B CN 202410135992 A CN202410135992 A CN 202410135992A CN 117701362 B CN117701362 B CN 117701362B
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- 238000000855 fermentation Methods 0.000 title claims abstract description 92
- 230000004151 fermentation Effects 0.000 title claims abstract description 92
- NVNLLIYOARQCIX-MSHCCFNRSA-N Nisin Chemical compound N1C(=O)[C@@H](CC(C)C)NC(=O)C(=C)NC(=O)[C@@H]([C@H](C)CC)NC(=O)[C@@H](NC(=O)C(=C/C)/NC(=O)[C@H](N)[C@H](C)CC)CSC[C@@H]1C(=O)N[C@@H]1C(=O)N2CCC[C@@H]2C(=O)NCC(=O)N[C@@H](C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(NCC(=O)N[C@H](C)C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCSC)C(=O)NCC(=O)N[C@H](CS[C@@H]2C)C(=O)N[C@H](CC(N)=O)C(=O)N[C@H](CCSC)C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(N[C@H](C)C(=O)N[C@@H]3C(=O)N[C@@H](C(N[C@H](CC=4NC=NC=4)C(=O)N[C@H](CS[C@@H]3C)C(=O)N[C@H](CO)C(=O)N[C@H]([C@H](C)CC)C(=O)N[C@H](CC=3NC=NC=3)C(=O)N[C@H](C(C)C)C(=O)NC(=C)C(=O)N[C@H](CCCCN)C(O)=O)=O)CS[C@@H]2C)=O)=O)CS[C@@H]1C NVNLLIYOARQCIX-MSHCCFNRSA-N 0.000 title claims abstract description 20
- 108010053775 Nisin Proteins 0.000 title claims abstract description 20
- 239000004309 nisin Substances 0.000 title claims abstract description 20
- 235000010297 nisin Nutrition 0.000 title claims abstract description 20
- 235000014897 Streptococcus lactis Nutrition 0.000 title claims abstract description 12
- 241000194035 Lactococcus lactis Species 0.000 title claims abstract 4
- 238000003756 stirring Methods 0.000 claims abstract description 67
- 238000005185 salting out Methods 0.000 claims abstract description 44
- 239000000919 ceramic Substances 0.000 claims abstract description 43
- 239000012528 membrane Substances 0.000 claims abstract description 43
- 238000001914 filtration Methods 0.000 claims abstract description 40
- 238000001035 drying Methods 0.000 claims abstract description 16
- 230000001105 regulatory effect Effects 0.000 claims abstract description 6
- 230000001276 controlling effect Effects 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 43
- 238000001179 sorption measurement Methods 0.000 claims description 21
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims 4
- 241000894006 Bacteria Species 0.000 claims 2
- 235000014655 lactic acid Nutrition 0.000 claims 2
- 239000004310 lactic acid Substances 0.000 claims 2
- 239000012535 impurity Substances 0.000 abstract description 16
- 230000000694 effects Effects 0.000 abstract description 13
- 235000019249 food preservative Nutrition 0.000 abstract description 2
- 239000005452 food preservative Substances 0.000 abstract description 2
- 244000057717 Streptococcus lactis Species 0.000 description 8
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 6
- 235000017491 Bambusa tulda Nutrition 0.000 description 6
- 241001330002 Bambuseae Species 0.000 description 6
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 6
- 239000011425 bamboo Substances 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000009514 concussion Effects 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses equipment for extracting nisin from lactococcus lactis fermentation broth, which relates to the technical field of biological food preservatives, wherein a first support frame is externally connected with a feeding mechanism and a program regulating and controlling system, a fifth support frame is externally connected with a drying mechanism, and a fermentation mechanism, a filtering mechanism, a concentration mechanism, a salting-out mechanism and a ceramic membrane mechanism are sequentially connected between the feeding mechanism and the drying mechanism; the fermentation mechanism comprises a fermentation unit, a first feeding unit and a first stirring unit, wherein the fermentation unit is arranged on a first support frame, the first feeding unit and the first stirring unit are both arranged on the fermentation unit, the first feeding unit is connected with the first stirring unit, and the first feeding unit is connected with an externally connected feeding mechanism; the filtering mechanism comprises a second feeding unit and a filtering and adsorbing unit; according to the invention, fermentation liquor can be fully fermented through the fermentation mechanism, the fermentation efficiency and effect are good, the filtering effect can be improved through the filtering mechanism, and the filtered impurities can be conveniently cleaned.
Description
Technical Field
The invention relates to the technical field of biological food preservatives, in particular to equipment for extracting nisin from lactococcus lactis fermentation broth.
Background
With the continuous development of society, food additives are increasingly used, and searching for safe and reliable preservatives becomes important; nisin has unique biological activity and good thermal stability, and is more and more important, and at present, nisin is mostly extracted from lactococcus lactis fermentation liquor when being prepared; in the prior art, when nisin is extracted from lactococcus lactis fermentation liquid, the fermentation efficiency is lower, the fermentation effect is not good enough, meanwhile, in the prior art, residual impurities are scraped off by a scraper when impurity is removed from filtering equipment, and then the residual impurities are washed, so that the process is complicated, and meanwhile, the nisin is not thoroughly separated due to poor stirring effect when stirring, so that the fermentation liquid is wasted.
The Chinese patent application with the application number of CN201810150346.8 proposes an extraction method of nisin, and the nisin is obtained by firstly preparing streptococcus lactis fermentation liquor, then regulating the pH value of the fermentation liquor, then carrying out processes of separation filtration, impurity removal and the like, and finally obtaining nisin.
Disclosure of Invention
Aiming at the technical problems, the invention provides equipment for extracting nisin from lactococcus lactis fermentation liquor, which comprises a first support frame, a second support frame, a third support frame, a fourth support frame and a fifth support frame, wherein the first support frame is externally connected with a feeding mechanism and a program regulating and controlling system, the fifth support frame is externally connected with a drying mechanism, and a fermentation mechanism, a filtering mechanism, a concentration mechanism, a salting-out mechanism and a ceramic membrane mechanism are sequentially connected between the feeding mechanism and the drying mechanism; the fermentation mechanism comprises a fermentation unit, a first feeding unit and a first stirring unit, wherein the fermentation unit is arranged on a first support frame, the first feeding unit and the first stirring unit are both arranged on the fermentation unit, the first feeding unit is connected with the first stirring unit, and the first feeding unit is connected with an externally connected feeding mechanism; the filtering mechanism comprises a second feeding unit and a filtering and adsorbing unit, the second feeding unit and the filtering and adsorbing unit are both arranged on a second supporting frame, the second feeding unit is connected with the filtering and adsorbing unit, and the fermentation unit is connected with the second feeding unit; the concentration mechanism is arranged on the third support frame, and the filtering and adsorbing unit is connected with the concentration mechanism; the salting-out mechanism is arranged on a support frame IV, the ceramic membrane mechanism is arranged on a support frame V, one end of the salting-out mechanism is connected with the concentration mechanism, the other end of the salting-out mechanism is connected with the ceramic membrane mechanism, and the ceramic membrane mechanism is connected with the external drying mechanism.
Further, fermentation unit includes fermentation cylinder, heater, feed cylinder and connecting pipe one, fermentation cylinder fixed mounting is on support frame one, heater fixed mounting is in the lower extreme of fermentation cylinder, feed cylinder fixed mounting is in the lower extreme of heater, connecting pipe one fixed mounting is in the lower extreme of feed cylinder, the junction of feed cylinder and connecting pipe one is provided with control valve one, be provided with PH value monitor and temperature monitor in the fermentation cylinder, the heater is the back taper.
Further, the first feeding unit comprises a first cover plate, a liquid inlet pipe, a first feeding pipe and a second feeding pipe, the first cover plate is detachably arranged at the upper end of the fermentation cylinder, the first liquid inlet pipe, the first feeding pipe and the second feeding pipe are fixedly arranged on the first cover plate and are communicated with the fermentation cylinder, and the first liquid inlet pipe, the first feeding pipe and the second feeding pipe are connected with an externally connected feeding mechanism.
Further, the first stirring unit comprises a motor I, a supporting seat I, a connecting shaft I, a mounting cylinder, a gear I, a connecting frame, a gear II, a gear III, a connecting rod, a stirring shaft, a stirring rod, a supporting shaft I, a supporting shaft II and a gear IV, wherein the supporting seat I is fixedly arranged on a cover plate I, the motor I is fixedly arranged on the supporting seat I, the connecting shaft I is fixedly arranged on an output shaft of the motor I, the mounting cylinder is fixedly arranged on the cover plate I, the gear is coaxially and fixedly arranged on the mounting cylinder, the connecting shaft I penetrates through the mounting cylinder, the connecting frame is fixedly arranged on the connecting shaft I, the supporting shaft II is rotatably arranged on the connecting frame, the gear II and the gear IV are coaxially and fixedly arranged on the support shaft II, the gear II is meshed with the gear I, the support shaft is rotatably arranged on the connecting frame, the gear III is fixedly arranged on the support shaft I, the gear III is meshed with the gear IV, the connecting rod is fixedly arranged on the support shaft I, the stirring shaft is fixedly arranged on the connecting rod, the stirring rods are fixedly arranged on the stirring shaft, a plurality of stirring rods are arranged on each stirring shaft, and the gear II, the gear III, the connecting rod, the stirring shaft, the support shaft I, the support shaft II and the gear IV are all arranged in three and uniformly distributed at intervals in a circumferential shape.
Further, filter adsorption unit includes a supporting section of thick bamboo, supporting seat two, inhale material pipe, connecting pipe four, centrifuging tube, briquetting, lifter, apron two, motor two, connecting axle two, belt pulley one, depression bar, belt pulley two, cartridge filter, adsorb first and the spring of fighting, a supporting section of thick bamboo, supporting seat two, motor two equal fixed mounting are on supporting frame two, inhale material pipe fixed mounting is on supporting seat two, it has the suction mechanism to inhale the material pipe external, four fixed mounting of connecting pipe are in the lower extreme of supporting the section of thick bamboo, apron two detachably installs the upper end at a supporting section of thick bamboo, the centrifuging tube rotates to be installed on apron two, coaxial fixed mounting of belt pulley is on the centrifuging tube, depression bar fixed mounting is on the centrifuging tube, the depression bar is provided with a plurality ofly, and is circumference form evenly distributed.
The lifting rod is slidably mounted on the cover plate II, the pressing block is fixedly mounted on the lifting rod, the lifting rod is an arc-shaped surface, the connecting shaft II is rotatably mounted on the support frame II, the connecting shaft II is fixedly connected with an output shaft of the motor II in a coaxial manner, the belt pulley is fixedly mounted on the connecting shaft II in a coaxial manner, a belt is connected between the belt pulley I and the belt pulley II, the filter cartridge is fixedly mounted on the lifting rod, the spring is sleeved on the lifting rod, one end of the spring is fixedly connected with the lifting rod, the other end of the spring is fixedly connected with the filter cartridge, the pressing block, the lifting rod and the spring are all provided with a plurality of pressing blocks and springs which are uniformly distributed on the cover plate II in a circumferential manner, and the lifting rods are uniformly distributed on the filter cartridge in a circumferential manner; be provided with a plurality of absorption on the side of centrifuging tube and fight two, just absorption fight two and centrifuging tube intercommunication, absorption fight one fixed mounting in the below of centrifuging tube, absorption fight one and centrifuging tube intercommunication, be provided with a plurality of filtration pores on the cartridge filter, the lower extreme of supporting the section of thick bamboo is provided with control valve two, be provided with control valve three on the material absorbing pipe, be provided with the striking piece on the inside of supporting the inside below of section of thick bamboo, the striking piece sets up the below at the cartridge filter.
Further, the second feeding unit comprises a second connecting pipe, a feeding hopper, a third connecting pipe and a third supporting seat, wherein the second connecting pipe is fixedly arranged on the third supporting seat, the feeding hopper is fixedly arranged on the second connecting pipe, the second connecting pipe is fixedly connected with the first connecting pipe, the upper end of the third connecting pipe is fixedly arranged on the feeding hopper, the lower end of the third connecting pipe is communicated with the centrifuge tube, the suction pipe is communicated with the third connecting pipe, and a fourth control valve is arranged on the second connecting pipe.
Further, the concentration mechanism comprises concentration equipment, a supporting seat IV, a connecting pipe V, a cover plate III, a connecting pipe V and a supporting seat V, wherein the concentration equipment is fixedly arranged on the supporting frame III, the cover plate III is detachably arranged at the upper end of the concentration equipment, the connecting pipe V is fixedly arranged on the supporting seat IV, one end of the connecting pipe V is fixedly connected with the connecting pipe V, the other end of the connecting pipe V is fixedly connected with the cover plate V, the connecting pipe V is communicated with the concentration equipment, the connecting pipe V is fixedly arranged at the lower end of the concentration equipment, the connecting pipe V is fixedly connected with the supporting seat V, and a control valve V is arranged at the lower end of the concentration equipment.
Further, salting out mechanism includes salting out equipment, connecting pipe seven, connecting pipe eight, supporting seat six, apron four, inlet pipe three and second stirring unit, salting out equipment fixed mounting is on supporting frame four, seven fixed mounting of connecting pipe is on supporting seat five, seven one end and connecting pipe six fixed connection of connecting pipe, seven other end and apron four of connecting pipe are connected, seven and salting out equipment intercommunication of connecting pipe, eight fixed mounting of connecting pipe is in salting out the lower extreme of equipment, eight and six fixed connection of supporting seat of connecting pipe, apron four detachably installs in salting out the upper end of equipment, three fixed mounting of inlet pipe is on apron four, three and salting out equipment intercommunication of inlet pipe, second stirring unit is installed on apron four, second stirring unit is the same with first stirring unit structure, salting out the lower extreme of equipment is provided with control valve six.
Further, ceramic membrane mechanism includes ceramic membrane equipment, connecting pipe nine, connecting pipe ten, supporting seat seven and apron five, ceramic membrane equipment fixed mounting is on support frame five, connecting pipe ten fixed mounting is at ceramic membrane equipment's lower extreme, apron five detachably installs in ceramic membrane equipment's upper end, connecting pipe ten and seven fixed connection of supporting seat, connecting pipe nine fixed mounting is on supporting seat six, connecting pipe nine's one end and eight fixed connection of connecting pipe, connecting pipe nine's the other end and apron five fixed connection, connecting pipe nine and ceramic membrane equipment intercommunication, connecting pipe ten is connected with external drying mechanism, ceramic membrane equipment's lower extreme is provided with control valve seven, connecting pipe two, connecting pipe five, connecting pipe seven, connecting pipe nine, connecting pipe ten are gone up and all are provided with the drawing liquid pump, supporting cylinder, concentrator, salting out equipment, ceramic membrane equipment's lower extreme all fall the toper.
Compared with the prior art, the invention has the beneficial effects that: (1) According to the invention, the stirring shaft and the stirring rod rotate around the axis of the gear and the axis of the supporting shaft at the same time, so that fermentation liquid in the fermentation cylinder can be fully stirred through the rotation of the stirring shaft and the stirring rod, the PH value and the temperature of the fermentation liquid are monitored through the PH value monitor and the temperature monitor arranged in the fermentation cylinder, the fermentation liquid is indirectly heated through the heater, the temperature uniformity is ensured, the stability of the fermentation liquid is not damaged, the fermentation liquid can be fermented better through the nutrient introduction of the liquid inlet pipe, the fermentation efficiency is higher, and the fermentation effect is better; (2) The centrifugal tube drives the first adsorption bucket and the second adsorption bucket to rotate when rotating, so that liquid in the centrifugal tube is thrown out of the first adsorption bucket and the second adsorption bucket under the action of centrifugal force, the liquid can uniformly pass through the filtering holes on the filter cylinder, the thrown-out liquid is filtered through the filter cylinder, and further the efficiency and the effect of liquid filtration can be improved; (3) The invention realizes the reciprocating oscillation of the filter cartridge and simultaneously can enable the filter cartridge to vibrate, thereby enabling the liquid passing through the filter cartridge to shake again and further improving the filtering effect on the fermentation liquor; (4) According to the invention, when impurities filtered on the filter cartridge are cleaned, the impurities filtered in the filter cartridge can be adsorbed into the centrifuge tube through the first adsorption hopper and the second adsorption hopper and adsorbed by the third connecting pipe and the suction pipe, and the filter cartridge vibrates while the second adsorption hopper rotates and the filter cartridge vibrates reciprocally, so that the impurities filtered in the filter cartridge can be fully sucked away, and the impurities remained on the inner wall of the filter cartridge can be shaken off when the filter cartridge vibrates, so that the cleaning efficiency and effect of the impurities in the filter cartridge are improved, the subsequent filtration of fermentation liquid is facilitated, the filter cartridge is not required to be detached for cleaning, the work load of people is reduced, the work efficiency is improved, and the cost is reduced.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present invention.
Fig. 2 is a top view of the present invention.
Fig. 3 is a front view of the present invention.
Fig. 4 is a side view of the present invention.
FIG. 5 is a schematic diagram of the structure of the fermentation mechanism of the present invention.
FIG. 6 is a schematic diagram showing a partial structure of a fermentation mechanism according to the present invention.
FIG. 7 is a schematic diagram showing a partial structure of a fermentation mechanism according to the present invention.
FIG. 8 is a schematic view of a filter mechanism according to the present invention.
Fig. 9 is an enlarged schematic view of the structure of fig. 8 a according to the present invention.
Fig. 10 is a top view of the filter mechanism of the present invention.
Fig. 11 is a cross-sectional view taken along A-A in fig. 10 in accordance with the present invention.
Fig. 12 is a top view of a second embodiment of the filter mechanism of the present invention.
Fig. 13 is a cross-sectional view taken along the direction B-B in fig. 12 in accordance with the present invention.
FIG. 14 is a schematic diagram showing the structures of a concentration mechanism, a salting-out mechanism and a ceramic membrane mechanism according to the present invention.
Fig. 15 is an enlarged view of the structure of fig. 14B according to the present invention.
Reference numerals: 101-fermenting cylinder; 102-a first support frame; 103-a heater; 104-blanking cylinder; 105-connecting the pipe I; 106-cover plate I; 107-liquid inlet pipe; 108-feeding pipe I; 109-motor one; 110-a first supporting seat; 111-connecting shaft I; 112-a second feeding pipe; 113-mounting a barrel; 114-gear one; 115-a connection rack; 116-gear two; 117-gear three; 118-connecting rods; 119-stirring shaft; 120-stirring rod; 121-a first supporting shaft; 122-a second supporting shaft; 123-gear four; 201-a support cylinder; 202-a second support frame; 203-a second supporting seat; 204-a suction pipe; 205-connecting the pipe II; 206-feeding hopper; 207-connecting tube three; 208-connecting the pipe IV; 209-a third supporting seat; 210-centrifuging tube; 211-briquetting; 212-lifting rod; 213-cover plate II; 214-a belt; 215-motor two; 216-connecting the second shaft; 217-pulley one; 218-a compression bar; 219-pulley two; 220-a filter cartridge; 221-adsorbing hopper I; 222-adsorbing a second bucket; 223-spring; 301-concentrating device; 302-a third support frame; 303-a fourth supporting seat; 304-connecting pipe five; 305-cover plate III; 306-connecting pipe six; 307-fifth support; 401-salting-out equipment; 402-a fourth supporting frame; 403-connecting tube seven; 404-connecting tube eight; 405-a sixth supporting seat; 406-cover plate four; 407-feed tube three; 501-ceramic membrane device; 502-a fifth supporting frame; 503-connecting pipe nine; 504-connecting tube ten; 505-support seat seven; 506-cover plate five.
Detailed Description
The invention will be further described with reference to specific examples, illustrative examples and illustrations of which are provided herein to illustrate the invention, but are not to be construed as limiting the invention.
Examples: the equipment for extracting nisin from lactococcus lactis fermentation liquor shown in the figures 1-15 comprises a first support frame 102, a second support frame 202, a third support frame 302, a fourth support frame 402 and a fifth support frame 502 which are used for supporting, wherein the first support frame 102 is externally connected with a feeding mechanism and a program regulating and controlling system, the fifth support frame 502 is externally connected with a drying mechanism, and a fermentation mechanism, a filtering mechanism, a concentration mechanism, a salting-out mechanism and a ceramic membrane mechanism are sequentially connected between the feeding mechanism and the drying mechanism; the fermentation mechanism comprises a fermentation unit, a first feeding unit and a first stirring unit, wherein the fermentation unit is arranged on a first support frame 102, the first feeding unit and the first stirring unit are both arranged on the fermentation unit, the first feeding unit is connected with the first stirring unit, and the first feeding unit is connected with an externally connected feeding mechanism; the filtering mechanism comprises a second feeding unit and a filtering and adsorbing unit, the second feeding unit and the filtering and adsorbing unit are both arranged on a second support frame 202, the second feeding unit is connected with the filtering and adsorbing unit, and the fermentation unit is connected with the second feeding unit; the concentration mechanism is arranged on the third support frame 302, and the filtering and adsorbing unit is connected with the concentration mechanism; the salting-out mechanism is arranged on the support frame IV 402, the ceramic membrane mechanism is arranged on the support frame V502, one end of the salting-out mechanism is connected with the concentration mechanism, the other end of the salting-out mechanism is connected with the ceramic membrane mechanism, and the ceramic membrane mechanism is connected with the external drying mechanism.
The fermentation unit comprises a fermentation cylinder 101, a heater 103, a blanking cylinder 104 and a connecting pipe I105, wherein the fermentation cylinder 101 is fixedly arranged on a support frame I102, the heater 103 is fixedly arranged at the lower end of the fermentation cylinder 101, the blanking cylinder 104 is fixedly arranged at the lower end of the heater 103, the connecting pipe I105 is fixedly arranged at the lower end of the blanking cylinder 104, a control valve I is arranged at the joint of the blanking cylinder 104 and the connecting pipe I105, a PH value monitor and a temperature monitor are arranged in the fermentation cylinder 101, and the heater 103 is in an inverted cone shape.
The first feeding unit comprises a first cover plate 106, a liquid inlet pipe 107, a first feeding pipe 108 and a second feeding pipe 112, the first cover plate 106 is detachably arranged at the upper end of the fermentation cylinder 101, the liquid inlet pipe 107, the first feeding pipe 108 and the second feeding pipe 112 are fixedly arranged on the first cover plate 106 and are communicated with the fermentation cylinder 101, and the liquid inlet pipe 107, the first feeding pipe 108 and the second feeding pipe 112 are connected with an externally connected feeding mechanism.
The first stirring unit comprises a first motor 109, a first supporting seat 110, a first connecting shaft 111, a mounting cylinder 113, a first gear 114, a connecting frame 115, a second gear 116, a third gear 117, a connecting rod 118, a stirring shaft 119, a stirring rod 120, a first supporting shaft 121, a second supporting shaft 122 and a fourth gear 123, wherein the first supporting seat 110 is fixedly arranged on a first cover plate 106, the first motor 109 is fixedly arranged on the first supporting seat 110, the first connecting shaft 111 is fixedly arranged on an output shaft of the first motor 109, the mounting cylinder 113 is fixedly arranged on the first cover plate 106, the first gear 114 is coaxially and fixedly arranged on the mounting cylinder 113, the first connecting shaft 111 passes through the mounting cylinder 113, the connecting frame 115 is fixedly arranged on the first connecting shaft 111, the second supporting shaft 122 is rotatably arranged on the connecting frame 115, the second gear 116 and the fourth gear 123 are coaxially and fixedly arranged on the second supporting shaft 122, the second gear 116 is meshed and connected with the first gear 114, the first supporting shaft 121 is rotatably arranged on the connecting frame 115, the third gear 117 is coaxially and fixedly arranged on the first supporting shaft 121, the third gear 117 is meshed and connected with the fourth gear 123, the connecting rod 118 is fixedly arranged on the first supporting shaft 121, the third stirring shaft 119 is uniformly arranged on the third connecting shaft 119 and is uniformly arranged on the circumference of the stirring shaft 119, the stirring shaft 119.
The filtering and adsorbing unit comprises a supporting cylinder 201, a supporting seat II 203, a suction pipe 204, a connecting pipe IV 208, a centrifuge tube 210, a pressing block 211, a lifting rod 212, a cover plate II 213, a motor II 215, a connecting shaft II 216, a belt pulley I217, a pressing rod 218, a belt pulley II 219, a filter cylinder 220, a first adsorption bucket 221 and a spring 223, wherein the supporting cylinder 201, the supporting seat II 203 and the motor II 215 are fixedly arranged on the supporting frame II 202, the suction pipe 204 is fixedly arranged on the supporting seat II 203, the suction pipe 204 is externally connected with a suction mechanism, the connecting pipe IV 208 is fixedly arranged at the lower end of the supporting cylinder 201, the cover plate II 213 is detachably arranged at the upper end of the supporting cylinder 201, the centrifuge tube 210 is rotatably arranged on the cover plate II 213, the belt pulley II 219 is coaxially and fixedly arranged on the centrifuge tube 210, the pressing rod 218 is fixedly arranged on the centrifuge tube 210, and the pressing rod 218 is provided with a plurality of the same and is uniformly distributed in a circumference shape;
Lifting rod 212 is slidably mounted on cover plate two 213, press block 211 is fixedly mounted on lifting rod 212, lifting rod 212 is an arc surface, connecting shaft two 216 is rotatably mounted on supporting frame two 202, connecting shaft two 216 is fixedly connected with the output shaft of motor two 215, belt pulley one 217 is fixedly mounted on connecting shaft two 216, belt 214 is connected between belt pulley one 217 and belt pulley two 219, filter cartridge 220 is fixedly mounted on lifting rod 212, spring 223 is sleeved on lifting rod 212, one end of spring 223 is fixedly connected with lifting rod 212, the other end of spring 223 is fixedly connected with filter cartridge 220, press block 211, lifting rod 212 and spring 223 are all provided with a plurality of press blocks 211 and springs 223 which are uniformly distributed on cover plate two 213 in a circumferential shape, and a plurality of lifting rods 212 are uniformly distributed on filter cartridge 220 in a circumferential shape; a plurality of second adsorption hoppers 222 are arranged on the side face of the centrifuge tube 210, the second adsorption hoppers 222 are communicated with the centrifuge tube 210, the first adsorption hoppers 221 are fixedly arranged below the centrifuge tube 210, the first adsorption hoppers 221 are communicated with the centrifuge tube 210, a plurality of filtering holes are formed in the filtering cylinder 220, a second control valve is arranged at the lower end of the supporting cylinder 201, a third control valve is arranged on the absorbing pipe 204, an impact block is arranged in the inner portion below the inner portion of the supporting cylinder 201, and the impact block is arranged below the filtering cylinder 220.
The second feeding unit comprises a second connecting pipe 205, a feed hopper 206, a third connecting pipe 207 and a third supporting seat 209, wherein the second connecting pipe 205 is fixedly arranged on the third supporting seat 209, the feed hopper 206 is fixedly arranged on the second connecting pipe 205, the second connecting pipe 205 is fixedly connected with the first connecting pipe 105, the upper end of the third connecting pipe 207 is fixedly arranged on the feed hopper 206, the lower end of the third connecting pipe 207 is communicated with a centrifugal pipe 210, the suction pipe 204 is communicated with the third connecting pipe 207, and a fourth control valve is arranged on the second connecting pipe 205.
The concentration mechanism comprises a concentration device 301, a support seat four 303, a connection pipe five 304, a cover plate three 305, a connection pipe six 306 and a support seat five 307, wherein the concentration device 301 is fixedly arranged on the support frame three 302, the cover plate three 305 is detachably arranged at the upper end of the concentration device 301, the connection pipe five 304 is fixedly arranged on the support seat four 303, one end of the connection pipe five 304 is fixedly connected with the connection pipe four 208, the other end of the connection pipe five 304 is fixedly connected with the cover plate three 305, the connection pipe five 304 is communicated with the concentration device 301, the connection pipe six 306 is fixedly arranged at the lower end of the concentration device 301, the connection pipe six 306 is fixedly connected with the support seat five 307, and a control valve five is arranged at the lower end of the concentration device 301.
The salting-out mechanism comprises salting-out equipment 401, a connecting pipe seven 403, a connecting pipe eight 404, a supporting seat six 405, a cover plate four 406, a feeding pipe three 407 and a second stirring unit, wherein the salting-out equipment 401 is fixedly arranged on the supporting frame four 402, the connecting pipe seven 403 is fixedly arranged on the supporting seat five 307, one end of the connecting pipe seven 403 is fixedly connected with the connecting pipe six 306, the other end of the connecting pipe seven 403 is connected with the cover plate four 406, the connecting pipe seven 403 is communicated with the salting-out equipment 401, the connecting pipe eight 404 is fixedly arranged at the lower end of the salting-out equipment 401, the connecting pipe eight 404 is fixedly connected with the supporting seat six 405, the cover plate four 406 is detachably arranged at the upper end of the salting-out equipment 401, the feeding pipe three 407 is fixedly arranged on the cover plate four 406, the feeding pipe three 407 is communicated with the salting-out equipment 401, the second stirring unit is arranged on the cover plate four 406, the second stirring unit is identical to the first stirring unit in structure, and the lower end of the salting-out equipment 401 is provided with a control valve six.
The ceramic membrane mechanism comprises a ceramic membrane device 501, a connecting pipe nine 503, a connecting pipe ten 504, a supporting seat seven 505 and a cover plate five 506, wherein the ceramic membrane device 501 is fixedly arranged on a supporting frame five 502, the connecting pipe ten 504 is fixedly arranged at the lower end of the ceramic membrane device 501, the cover plate five 506 is detachably arranged at the upper end of the ceramic membrane device 501, the connecting pipe ten 504 is fixedly connected with the supporting seat seven 505, the connecting pipe nine 503 is fixedly arranged on the supporting seat six 405, one end of the connecting pipe nine 503 is fixedly connected with the connecting pipe eight 404, the other end of the connecting pipe nine 503 is fixedly connected with the cover plate five 506, the connecting pipe nine 503 is communicated with the ceramic membrane device 501, the connecting pipe ten 504 is connected with an externally connected drying mechanism, a control valve seven is arranged at the lower end of the ceramic membrane device 501, and the connecting pipe two 205, the connecting pipe five 304, the connecting pipe seven 403, the connecting pipe nine 503 and the connecting pipe ten 504 are all provided with liquid pumps, the supporting cylinder 201, the concentration device 301, the salting-out device 401 and the lower end of the ceramic membrane device 501 are all reversely conical.
The working principle of the invention is as follows: the fermentation mechanism works: the lactococcus lactis fermentation liquid is introduced into the fermentation cylinder 101 through a second feeding pipe 112 by an external feeding mechanism, and the reagent for adjusting the PH value of the fermentation liquid is introduced through a first feeding pipe 108.
The output shaft of the motor I109 drives the connecting shaft I111 to rotate, the connecting frame 115 is driven to rotate when the connecting shaft I111 rotates, the gear II 116 is driven to rotate around the axis of the gear I114 when the connecting frame 115 rotates, the gear II 116 drives the gear IV 123 to rotate through the supporting shaft II 122, the gear IV 123 drives the supporting shaft I121 to rotate through the gear III 117, the supporting shaft I121 drives the stirring shaft 119 to rotate through the connecting rod 118, the stirring shaft 119 drives the stirring rod 120 to rotate around the axis of the supporting shaft I121 when the stirring shaft 119 and the stirring rod 120 rotate around the axis of the gear I114, fermentation liquid in the fermentation cylinder 101 can be fully stirred through the rotation of the stirring shaft 119 and the stirring rod 120, the PH value and the temperature of the fermentation liquid can be monitored through the PH value monitor and the temperature monitor arranged in the fermentation cylinder 101, the fermentation liquid can be indirectly heated through the heater 103, the temperature is uniform and the stability of the fermentation liquid is not damaged, nutrients are introduced through the liquid inlet pipe 107, and the fermentation efficiency is high, and the fermentation effect is good.
The filter mechanism works: closing a control valve III on the suction pipe 204, opening a control valve IV arranged on a connecting pipe II 205, enabling fermented solution to enter a feed hopper 206 through a connecting pipe II 205, enabling the fermented solution to enter a centrifugal pipe 210 through a connecting pipe III 207, enabling an output shaft of a motor II 215 to drive a belt pulley I217 to rotate through a connecting shaft II 216, enabling the belt pulley I217 to drive a belt pulley II 219 to rotate through a belt 214, enabling the belt pulley II 219 to drive the centrifugal pipe 210 to rotate, enabling the centrifugal pipe 210 to drive a compression bar 218 to rotate, enabling a suction hopper I221 and a suction hopper II 222 to rotate when the centrifugal pipe 210 rotates, enabling liquid in the centrifugal pipe 210 to be thrown out from the suction hopper I221 and the suction hopper II 222 under the action of centrifugal force, enabling the liquid to uniformly pass through filter holes in a filter cartridge 220, and enabling thrown-out liquid to be filtered through the filter cartridge 220, and further improving the efficiency and effect of liquid filtration.
When pressure bar 218 rotates to the position of pressure block 211, drive lifter 212 through pressure block 211 and remove, lifter 212 drives cartridge filter 220 and removes, when cartridge filter 220 runs into on the inner wall of support cylinder 201 below, bump through the striking piece on cartridge filter 220 and the support cylinder 201 below inner wall, when pressure bar 218 breaks away from with pressure block 211, under the effect of spring 223 elasticity, cartridge filter 220 resumes the normal position, and then can make cartridge filter 220 take place vibrations when having realized the reciprocal concussion of cartridge filter 220, thereby can make the liquid through cartridge filter 220 take place to rock once more, further improved the filter effect to the fermentation broth.
When the filtered impurities on the filter cartridge 220 need to be cleaned after the filtration is completed, the control valve IV on the connecting pipe II 205 is closed, the control valve III on the suction pipe 204 is opened, the suction mechanism externally connected with the suction pipe 204 is used for sucking air, and meanwhile, the centrifuge tube 210 is rotated, so that the filter cartridge 220 is vibrated (the centrifuge tube 210 rotates and the working principle of the movement of the filter cartridge 220 is referred to before), the filtered impurities in the filter cartridge 220 are adsorbed into the centrifuge tube 210 through the adsorption hopper I221 and the adsorption hopper II 222, the filtered impurities are adsorbed by the connecting pipe III 207 and the suction pipe 204, and the filter cartridge 220 is vibrated while the rotation of the adsorption hopper II 222 and the reciprocating vibration of the filter cartridge 220 are performed, so that the filtered impurities in the filter cartridge 220 can be sufficiently sucked, and the residual impurities on the inner wall of the filter cartridge 220 can be shaken off when the filter cartridge 220 vibrates, thereby improving the cleaning efficiency and the effect of the impurities in the filter cartridge 220, facilitating the filtration of the subsequent fermentation liquid, simultaneously, improving the working efficiency while reducing the work load of people and reducing the cost.
The concentration mechanism, salting out mechanism, ceramic membrane mechanism, dry mechanism during operation: the liquid filtered by the filtering mechanism enters a connecting pipe five 304 through a connecting pipe four 208, enters a concentrating device 301 through the connecting pipe five 304, is concentrated through the concentrating device 301, enters a connecting pipe seven 403 through a connecting pipe six 306, enters a salting-out device 401 through the connecting pipe seven 403, is added with salt through a feeding pipe three 407 into the salting-out device 401, is dissolved and promotes nisin to precipitate through a second stirring unit (the same as the working principle of the first stirring unit), the precipitated liquid enters a connecting pipe nine 503 through a connecting pipe eight 404, enters a ceramic membrane device 501 through the connecting pipe nine 503 for further purification, and the purified liquid enters a drying mechanism through a connecting pipe ten 504 for drying, so that nisin is made into a form easy to store and transport, and the nisin extraction is completed.
It should be specifically noted that the above working process and the start and stop of the power part are regulated and controlled by the program regulation and control system.
Claims (4)
1. The equipment for extracting nisin from lactococcus lactis fermentation liquor comprises a first support frame (102), a second support frame (202), a third support frame (302), a fourth support frame (402) and a fifth support frame (502), wherein the first support frame (102) is externally connected with a feeding mechanism and a program regulating and controlling system, and the fifth support frame (502) is externally connected with a drying mechanism, and the equipment is characterized in that a fermentation mechanism, a filtering mechanism, a concentration mechanism, a salting-out mechanism and a ceramic membrane mechanism are sequentially connected between the feeding mechanism and the drying mechanism; the fermentation mechanism comprises a fermentation unit, a first feeding unit and a first stirring unit, wherein the fermentation unit is arranged on a first supporting frame (102), the first feeding unit and the first stirring unit are both arranged on the fermentation unit, the first feeding unit is connected with the first stirring unit, and the first feeding unit is connected with an externally connected feeding mechanism; the filtering mechanism comprises a second feeding unit and a filtering and adsorbing unit, the second feeding unit and the filtering and adsorbing unit are both arranged on a second supporting frame (202), the second feeding unit is connected with the filtering and adsorbing unit, and the fermentation unit is connected with the second feeding unit; the concentration mechanism is arranged on a third supporting frame (302), and the filtering and adsorbing unit is connected with the concentration mechanism; the salting-out mechanism is arranged on a fourth support frame (402), the ceramic membrane mechanism is arranged on a fifth support frame (502), one end of the salting-out mechanism is connected with the concentration mechanism, the other end of the salting-out mechanism is connected with the ceramic membrane mechanism, and the ceramic membrane mechanism is connected with an external drying mechanism;
The fermentation unit comprises a fermentation cylinder (101), a heater (103), a blanking cylinder (104) and a first connecting pipe (105), wherein the fermentation cylinder (101) is fixedly arranged on a first supporting frame (102), the heater (103) is fixedly arranged at the lower end of the fermentation cylinder (101), the blanking cylinder (104) is fixedly arranged at the lower end of the heater (103), the first connecting pipe (105) is fixedly arranged at the lower end of the blanking cylinder (104), a first control valve is arranged at the joint of the blanking cylinder (104) and the first connecting pipe (105), a PH value monitor and a temperature monitor are arranged in the fermentation cylinder (101), and the heater (103) is in an inverted cone shape;
The first feeding unit comprises a cover plate I (106), a liquid inlet pipe (107), a first feeding pipe (108) and a second feeding pipe (112), wherein the cover plate I (106) is detachably arranged at the upper end of the fermentation cylinder (101), the liquid inlet pipe (107), the first feeding pipe (108) and the second feeding pipe (112) are fixedly arranged on the cover plate I (106) and are communicated with the fermentation cylinder (101), and the liquid inlet pipe (107), the first feeding pipe (108) and the second feeding pipe (112) are connected with an externally-connected feeding mechanism;
The first stirring unit comprises a first motor (109), a first supporting seat (110), a first connecting shaft (111), a mounting cylinder (113), a first gear (114), a connecting frame (115), a second gear (116), a third gear (117), a connecting rod (118), a stirring shaft (119), a stirring rod (120), a first supporting shaft (121), a second supporting shaft (122) and a fourth gear (123), wherein the first supporting seat (110) is fixedly arranged on a first cover plate (106), the first motor (109) is fixedly arranged on the first supporting seat (110), the first connecting shaft (111) is fixedly arranged on an output shaft of the first motor (109), the mounting cylinder (113) is fixedly arranged on a first cover plate (106), the first gear (114) is coaxially and fixedly arranged on the mounting cylinder (113), the first connecting shaft (111) penetrates through the mounting cylinder (113), the connecting frame (115) is fixedly arranged on the first connecting shaft (111), the second supporting shaft (122) is rotatably arranged on the connecting frame (115), the second gear (116) and the fourth gear (123) are fixedly arranged on the first supporting shaft (121) and the first gear (121) in a meshed mode, the gear III (117) is coaxially and fixedly arranged on the first supporting shaft (121), the gear III (117) is meshed with the fourth gear (123), the connecting rod (118) is fixedly arranged on the first supporting shaft (121), the stirring shafts (119) are fixedly arranged on the connecting rod (118), the stirring rods (120) are fixedly arranged on the stirring shafts (119), a plurality of stirring rods (120) are arranged on each stirring shaft (119), and the second gear (116), the third gear (117), the connecting rod (118), the stirring shafts (119), the first supporting shaft (121), the second supporting shaft (122) and the fourth gear (123) are all arranged in three and uniformly distributed at intervals in a circumferential shape;
The filtering and adsorbing unit comprises a supporting cylinder (201), a supporting seat II (203), a suction pipe (204), a connecting pipe IV (208), a centrifugal pipe (210), a pressing block (211), a lifting rod (212), a cover plate II (213), a motor II (215), a connecting shaft II (216), a belt pulley I (217), a pressing rod (218), a belt pulley II (219), a filter cylinder (220), an adsorption bucket I (221) and a spring (223), wherein the supporting cylinder (201), the supporting seat II (203) and the motor II (215) are fixedly arranged on the supporting frame II (202), the suction pipe (204) is fixedly arranged on the supporting seat II (203), the suction pipe (204) is externally connected with a suction mechanism, the connecting pipe IV (208) is fixedly arranged at the lower end of the supporting cylinder (201), the cover plate II (213) is detachably arranged at the upper end of the supporting cylinder (201), the centrifugal pipe (210) is rotatably arranged on the cover plate II (213), the belt pulley II (219) is coaxially and fixedly arranged on the supporting cylinder (210), the pressing rod (218) is fixedly arranged on the centrifugal pipe (210), and the pressing rods (218) are uniformly distributed in a circular shape. The lifting rod (212) is slidably mounted on the cover plate II (213), the pressing block (211) is fixedly mounted on the lifting rod (212), the lifting rod (212) is an arc surface, the connecting shaft II (216) is rotatably mounted on the support frame II (202), the connecting shaft II (216) is fixedly connected with an output shaft of the motor II (215) coaxially, the belt pulley I (217) is fixedly mounted on the connecting shaft II (216) coaxially, a belt (214) is connected between the belt pulley I (217) and the belt pulley II (219), the filter cartridge (220) is fixedly mounted on the lifting rod (212), the spring (223) is sleeved on the lifting rod (212), one end of the spring (223) is fixedly connected with the lifting rod (212), the other end of the spring (223) is fixedly connected with the filter cartridge (220), the pressing block (211), the lifting rod (212) and the spring (223) are all provided with a plurality, the pressing block (211) and the spring (223) are uniformly distributed on the circumference of the cover plate II (213) in a uniform circumference, and the plurality of filter cartridges (220) are uniformly distributed on the circumference of the lifting rod (212); a plurality of second adsorption hoppers (222) are arranged on the side face of the centrifuge tube (210), the second adsorption hoppers (222) are communicated with the centrifuge tube (210), the first adsorption hoppers (221) are fixedly arranged below the centrifuge tube (210), the first adsorption hoppers (221) are communicated with the centrifuge tube (210), a plurality of filtering holes are formed in the filter cylinder (220), a second control valve is arranged at the lower end of the support cylinder (201), a third control valve is arranged on the suction tube (204), an impact block is arranged inside the inner lower portion of the support cylinder (201), and the impact block is arranged below the filter cylinder (220);
The second feeding unit comprises a second connecting pipe (205), a feed hopper (206), a third connecting pipe (207) and a third supporting seat (209), wherein the second connecting pipe (205) is fixedly installed on the third supporting seat (209), the feed hopper (206) is fixedly installed on the second connecting pipe (205), the second connecting pipe (205) is fixedly connected with the first connecting pipe (105), the upper end of the third connecting pipe (207) is fixedly installed on the feed hopper (206), the lower end of the third connecting pipe (207) is communicated with the centrifuge tube (210), the suction pipe (204) is communicated with the third connecting pipe (207), and a fourth control valve is arranged on the second connecting pipe (205).
2. The apparatus for extracting nisin from a lactic acid bacterium fermentation broth according to claim 1, wherein the concentration mechanism comprises a concentration apparatus (301), a fourth support seat (303), a fifth connection pipe (304), a third cover plate (305), a sixth connection pipe (306) and a fifth support seat (307), the concentration apparatus (301) is fixedly mounted on the third support seat (302), the third cover plate (305) is detachably mounted at the upper end of the concentration apparatus (301), the fifth connection pipe (304) is fixedly mounted on the fourth support seat (303), one end of the fifth connection pipe (304) is fixedly connected with the fourth connection pipe (208), the other end of the fifth connection pipe (304) is fixedly connected with the third cover plate (305), the fifth connection pipe (304) is communicated with the concentration apparatus (301), the sixth connection pipe (306) is fixedly mounted at the lower end of the concentration apparatus (301), the sixth connection pipe (306) is fixedly connected with the fifth support seat (307), and the lower end of the concentration apparatus (301) is provided with a control valve (five).
3. An apparatus for extracting nisin from a lactic acid bacteria fermentation broth as claimed in claim 2, wherein the salting-out means comprises a salting-out apparatus (401), a connection pipe seven (403), a connection pipe eight (404), a support seat six (405), a cover plate four (406), a connection pipe three (407) and a second stirring unit, wherein the salting-out apparatus (401) is fixedly mounted on the support frame four (402), the connection pipe seven (403) is fixedly mounted on the support seat five (307), one end of the connection pipe seven (403) is fixedly connected with the connection pipe six (306), the other end of the connection pipe seven (403) is connected with the cover plate four (406), the connection pipe seven (403) is communicated with the salting-out apparatus (401), the connection pipe eight (404) is fixedly mounted at the lower end of the salting-out apparatus (401), the cover plate four (406) is detachably mounted at the upper end of the salting-out apparatus (401), the connection pipe three (407) is fixedly mounted on the cover plate four (406), the third stirring unit is mounted at the lower end of the salting-out apparatus (401), and the second stirring unit is mounted at the same stirring unit (401), and the first stirring unit is connected with the second stirring unit (401).
4. An apparatus for extracting nisin from a fermentation broth of lactococcus lactis as claimed in claim 3, wherein the ceramic membrane mechanism comprises a ceramic membrane apparatus (501), a connecting pipe nine (503), a connecting pipe ten (504), a supporting seat seven (505) and a cover plate five (506), wherein the ceramic membrane apparatus (501) is fixedly arranged on a supporting frame five (502), the connecting pipe ten (504) is fixedly arranged at the lower end of the ceramic membrane apparatus (501), the cover plate five (506) is detachably arranged at the upper end of the ceramic membrane apparatus (501), the connecting pipe ten (504) is fixedly connected with the supporting seat seven (505), the ceramic membrane device comprises a support seat six (405), a connecting pipe nine (503), a connecting pipe eight (404), a connecting pipe eight (503), a connecting pipe eight (504) and a ceramic membrane device (501), wherein the connecting pipe nine (503) is fixedly arranged on the support seat six (405), one end of the connecting pipe nine (503) is fixedly connected with the connecting pipe eight (404), the other end of the connecting pipe nine (503) is fixedly connected with the cover plate five (506), the connecting pipe nine (503) is communicated with the ceramic membrane device (501), the connecting pipe ten (504) is connected with an external drying mechanism, the lower end of the ceramic membrane device (501) is provided with a control valve seven, and liquid pumps are arranged on the connecting pipe two (205), the connecting pipe five (304), the connecting pipe seven (403), the connecting pipe nine (503) and the connecting pipe ten (504), the supporting cylinder (201), the concentrating device (301), salting out device (401), the lower ends of the ceramic membrane devices (501) are all reversely tapered.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410135992.2A CN117701362B (en) | 2024-01-31 | 2024-01-31 | Equipment for extracting nisin from lactococcus lactis fermentation liquor |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410135992.2A CN117701362B (en) | 2024-01-31 | 2024-01-31 | Equipment for extracting nisin from lactococcus lactis fermentation liquor |
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| CN117701362A CN117701362A (en) | 2024-03-15 |
| CN117701362B true CN117701362B (en) | 2024-04-23 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202410135992.2A Active CN117701362B (en) | 2024-01-31 | 2024-01-31 | Equipment for extracting nisin from lactococcus lactis fermentation liquor |
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| CN220079002U (en) * | 2023-05-29 | 2023-11-24 | 谷梁和香(成都)酿酒研究院 | Filter equipment that making wine fermentation was used |
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| KR200384843Y1 (en) * | 2005-03-02 | 2005-05-19 | 대영이앤비 주식회사 | Agitator gear moving system of food waste disposer |
| CN205170827U (en) * | 2014-12-03 | 2016-04-20 | 武汉科技大学 | Automatic fermentation cylinder agitating unit of mechanical type |
| CN109266709A (en) * | 2017-07-17 | 2019-01-25 | 四川安益生物科技有限公司 | Nisin production technology |
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|---|---|
| CN117701362A (en) | 2024-03-15 |
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