CN117624344A - 一种重组人源化金属硫蛋白的制备方法及其应用 - Google Patents
一种重组人源化金属硫蛋白的制备方法及其应用 Download PDFInfo
- Publication number
- CN117624344A CN117624344A CN202311661822.XA CN202311661822A CN117624344A CN 117624344 A CN117624344 A CN 117624344A CN 202311661822 A CN202311661822 A CN 202311661822A CN 117624344 A CN117624344 A CN 117624344A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- metallothionein
- iptg
- medium
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000003792 Metallothionein Human genes 0.000 title claims abstract description 60
- 108090000157 Metallothionein Proteins 0.000 title claims abstract description 60
- DIGQNXIGRZPYDK-WKSCXVIASA-N (2R)-6-amino-2-[[2-[[(2S)-2-[[2-[[(2R)-2-[[(2S)-2-[[(2R,3S)-2-[[2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S,3S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2R)-2-[[2-[[2-[[2-[(2-amino-1-hydroxyethylidene)amino]-3-carboxy-1-hydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1,5-dihydroxy-5-iminopentylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]hexanoic acid Chemical compound C[C@@H]([C@@H](C(=N[C@@H](CS)C(=N[C@@H](C)C(=N[C@@H](CO)C(=NCC(=N[C@@H](CCC(=N)O)C(=NC(CS)C(=N[C@H]([C@H](C)O)C(=N[C@H](CS)C(=N[C@H](CO)C(=NCC(=N[C@H](CS)C(=NCC(=N[C@H](CCCCN)C(=O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)N=C([C@H](CS)N=C([C@H](CO)N=C([C@H](CO)N=C([C@H](C)N=C(CN=C([C@H](CO)N=C([C@H](CS)N=C(CN=C(C(CS)N=C(C(CC(=O)O)N=C(CN)O)O)O)O)O)O)O)O)O)O)O)O DIGQNXIGRZPYDK-WKSCXVIASA-N 0.000 title claims abstract description 57
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 238000000855 fermentation Methods 0.000 claims abstract description 122
- 230000004151 fermentation Effects 0.000 claims abstract description 117
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 claims abstract description 42
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 claims abstract description 40
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims abstract description 38
- OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 claims abstract description 21
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 21
- 229960003692 gamma aminobutyric acid Drugs 0.000 claims abstract description 21
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 21
- 239000001301 oxygen Substances 0.000 claims abstract description 21
- 108010024636 Glutathione Proteins 0.000 claims abstract description 17
- 229960003180 glutathione Drugs 0.000 claims abstract description 17
- 239000006228 supernatant Substances 0.000 claims abstract description 15
- 239000007788 liquid Substances 0.000 claims description 32
- 239000002609 medium Substances 0.000 claims description 30
- 108090000623 proteins and genes Proteins 0.000 claims description 20
- 241000894006 Bacteria Species 0.000 claims description 18
- 238000002835 absorbance Methods 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 13
- 238000003756 stirring Methods 0.000 claims description 10
- 230000006698 induction Effects 0.000 claims description 8
- 239000013612 plasmid Substances 0.000 claims description 8
- 241000588724 Escherichia coli Species 0.000 claims description 7
- 238000011081 inoculation Methods 0.000 claims description 6
- 239000007222 ypd medium Substances 0.000 claims description 4
- 239000000047 product Substances 0.000 claims description 2
- 239000003002 pH adjusting agent Substances 0.000 claims 1
- 241001052560 Thallis Species 0.000 abstract description 3
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract description 2
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 33
- 102000004169 proteins and genes Human genes 0.000 description 16
- 239000001963 growth medium Substances 0.000 description 15
- 239000000243 solution Substances 0.000 description 15
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 13
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 235000018102 proteins Nutrition 0.000 description 9
- 230000001580 bacterial effect Effects 0.000 description 6
- 230000003716 rejuvenation Effects 0.000 description 6
- 239000011780 sodium chloride Substances 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 5
- 229960000723 ampicillin Drugs 0.000 description 5
- 101000708016 Caenorhabditis elegans Sentrin-specific protease Proteins 0.000 description 4
- 239000001888 Peptone Substances 0.000 description 4
- 108010080698 Peptones Proteins 0.000 description 4
- 229920005654 Sephadex Polymers 0.000 description 4
- 239000012507 Sephadex™ Substances 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 229940041514 candida albicans extract Drugs 0.000 description 4
- 230000029087 digestion Effects 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 108020001507 fusion proteins Proteins 0.000 description 4
- 102000037865 fusion proteins Human genes 0.000 description 4
- 239000012535 impurity Substances 0.000 description 4
- 239000002808 molecular sieve Substances 0.000 description 4
- 235000019319 peptone Nutrition 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 4
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 239000011550 stock solution Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 239000012138 yeast extract Substances 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- RWSXRVCMGQZWBV-PHDIDXHHSA-N L-Glutathione Natural products OC(=O)[C@H](N)CCC(=O)N[C@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-PHDIDXHHSA-N 0.000 description 3
- 229940098773 bovine serum albumin Drugs 0.000 description 3
- 229910001385 heavy metal Inorganic materials 0.000 description 3
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000011218 seed culture Methods 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000194035 Lactococcus lactis Species 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 235000014897 Streptococcus lactis Nutrition 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 238000003259 recombinant expression Methods 0.000 description 2
- 230000001502 supplementing effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- DJQYYYCQOZMCRC-UHFFFAOYSA-N 2-aminopropane-1,3-dithiol Chemical group SCC(N)CS DJQYYYCQOZMCRC-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 102000010750 Metalloproteins Human genes 0.000 description 1
- 108010063312 Metalloproteins Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- YVNQAIFQFWTPLQ-UHFFFAOYSA-O [4-[[4-(4-ethoxyanilino)phenyl]-[4-[ethyl-[(3-sulfophenyl)methyl]amino]-2-methylphenyl]methylidene]-3-methylcyclohexa-2,5-dien-1-ylidene]-ethyl-[(3-sulfophenyl)methyl]azanium Chemical compound C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C(=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S(O)(=O)=O)C)C=2C(=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S(O)(=O)=O)C)C=C1 YVNQAIFQFWTPLQ-UHFFFAOYSA-O 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 239000012152 bradford reagent Substances 0.000 description 1
- 230000009920 chelation Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 239000013028 medium composition Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 229910021654 trace metal Inorganic materials 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/825—Metallothioneins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/185—Escherichia
- C12R2001/19—Escherichia coli
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Birds (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Epidemiology (AREA)
- General Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
金属硫蛋白得率(mg/ml) | |
实施例1 | 0.769 |
实施例2 | 0.776 |
实施例3 | 0.813 |
对比例1 | 0.441 |
对比例2 | 0.489 |
对比例3 | 0.502 |
对比例4 | 0.633 |
对比例5 | 0.468 |
对比例6 | 0.530 |
对比例7 | 0.662 |
对比例8 | 0.637 |
金属硫蛋白百分比(%) | |
实施例1 | 10.23 |
实施例2 | 11.57 |
实施例3 | 11.26 |
对比例1 | 7.82 |
对比例2 | 8.05 |
对比例3 | 7.22 |
对比例4 | 8.74 |
对比例5 | 8.82 |
对比例6 | 9.19 |
对比例7 | 8.64 |
对比例8 | 8.51 |
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202311661822.XA CN117624344A (zh) | 2023-12-06 | 2023-12-06 | 一种重组人源化金属硫蛋白的制备方法及其应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202311661822.XA CN117624344A (zh) | 2023-12-06 | 2023-12-06 | 一种重组人源化金属硫蛋白的制备方法及其应用 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN117624344A true CN117624344A (zh) | 2024-03-01 |
Family
ID=90030241
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202311661822.XA Pending CN117624344A (zh) | 2023-12-06 | 2023-12-06 | 一种重组人源化金属硫蛋白的制备方法及其应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN117624344A (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN118307664A (zh) * | 2024-06-11 | 2024-07-09 | 英特菲尔(成都)生物制品有限责任公司 | 一种重组人金属硫蛋白及其制备方法与应用 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101003806A (zh) * | 2006-12-31 | 2007-07-25 | 吉林农业大学 | 利用植物油体表达人金属硫蛋白的方法 |
CN102181468A (zh) * | 2011-01-26 | 2011-09-14 | 暨南大学 | 酿酒酵母金属硫蛋白成熟肽的高效表达纯化方法 |
CN103898153A (zh) * | 2012-12-28 | 2014-07-02 | 山东东兴生物科技股份有限公司 | 一种多拷贝的金属硫蛋白重组表达载体及其高效表达金属硫蛋白的方法 |
-
2023
- 2023-12-06 CN CN202311661822.XA patent/CN117624344A/zh active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101003806A (zh) * | 2006-12-31 | 2007-07-25 | 吉林农业大学 | 利用植物油体表达人金属硫蛋白的方法 |
CN102181468A (zh) * | 2011-01-26 | 2011-09-14 | 暨南大学 | 酿酒酵母金属硫蛋白成熟肽的高效表达纯化方法 |
CN103898153A (zh) * | 2012-12-28 | 2014-07-02 | 山东东兴生物科技股份有限公司 | 一种多拷贝的金属硫蛋白重组表达载体及其高效表达金属硫蛋白的方法 |
Non-Patent Citations (2)
Title |
---|
彭冬;杨威;王昭;王席;柳忠玉;: "GST-SUMO-MT融合蛋白在大肠杆菌中的自诱导表达", 生物技术通报, no. 02, 26 February 2016 (2016-02-26), pages 219 - 224 * |
黄亚东等: "融和蛋白GST-SUMO-MT在大肠杆菌中的表达、纯化及其活性研究", 中国生物工程杂志, no. 12, 15 December 2007 (2007-12-15), pages 11 - 16 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN118307664A (zh) * | 2024-06-11 | 2024-07-09 | 英特菲尔(成都)生物制品有限责任公司 | 一种重组人金属硫蛋白及其制备方法与应用 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US9017985B2 (en) | Photosynthetic microorganisms enriched in selenium using selenohydroxy acid compounds, used thereof in nutrition, cosmetics and pharmacy | |
CN112553120B (zh) | 一株大肠埃希氏菌及其在产聚唾液酸中的应用 | |
CN114015729B (zh) | 植物乳杆菌在制备乳酸菌素、乳酸和苯乳酸中的应用 | |
CN116496950B (zh) | 一株赖氨酸生产菌株及用途,生产赖氨酸的方法 | |
CN117089503B (zh) | 一种大肠杆菌k-12 mg1655 blbyzt6及其应用 | |
EP4442694A1 (en) | New crystal form of trisaccharide | |
CN107699505B (zh) | 一种富硒酵母培养工艺的优化方法及工艺 | |
CN113321580B (zh) | 一种生产苹果酸的方法 | |
CN113046253B (zh) | 一种提高马克斯克鲁维酵母耐热性的培养方法 | |
CN117624344A (zh) | 一种重组人源化金属硫蛋白的制备方法及其应用 | |
CN115895976B (zh) | 一株产l-色氨酸的大肠杆菌及其生产l-色氨酸的应用 | |
CN101984066A (zh) | 一种生物法生产l-苯丙氨酸的方法 | |
CN107446909B (zh) | 一种大肠杆菌的固定化方法及利用固定化大肠杆菌补料发酵生产l-赖氨酸的方法 | |
CN109136299A (zh) | 一种制备、提取以及纯化苏氨酸的方法 | |
CN116333948A (zh) | 一种食气梭菌增菌培养基及其制备方法 | |
CN115651918A (zh) | 一种提高葡萄糖氧化酶产量的方法 | |
CN114634882B (zh) | 一种促进酵母生长和发酵的方法 | |
CN110283855B (zh) | 一种提高嗜热链球菌210202菌株产gaba能力的方法 | |
CN110527705B (zh) | 一种酶解兔血发酵液制备抗氧化低聚肽的方法 | |
CN113981021A (zh) | 一种通过嗜氨副球菌发酵合成pqq的方法 | |
CN110564805A (zh) | 一种兔血抗氧化肽的发酵制备方法 | |
CN110724726A (zh) | 一种鱼肉腐败菌氨基酸脱氨能力的检测方法 | |
CN118256398B (zh) | 高产谷氨酰胺的副干酪乳酪杆菌及其应用 | |
CN114873739B (zh) | 一种亚硝酸盐还原酶促进剂及其应用 | |
CN114540248B (zh) | 甲烷菌lgm-dz1及其分离纯化方法和应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20240415 Address after: 510000, 108, No. 2 Tianyuan East Road, Huangpu District, Guangzhou City, Guangdong Province Applicant after: Guangdong Saiyuan Biomedical Technology Co.,Ltd. Country or region after: China Address before: Room 401, No. 2 Tianyuan East Road, Huangpu District, Guangzhou City, Guangdong Province, 510405 Applicant before: Guangzhou boshipai Biotechnology Co.,Ltd. Country or region before: China |
|
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20240619 Address after: 510000 307, No. 6, Yongjia Road, Jiahe street, Baiyun District, Guangzhou City, Guangdong Province Applicant after: Guangzhou boshipai Biotechnology Co.,Ltd. Country or region after: China Address before: 510000, 108, No. 2 Tianyuan East Road, Huangpu District, Guangzhou City, Guangdong Province Applicant before: Guangdong Saiyuan Biomedical Technology Co.,Ltd. Country or region before: China |
|
TA01 | Transfer of patent application right |