CN117617256A - Nematicidal composition and application thereof in preventing and controlling nematode infection and development on crops - Google Patents

Abstract

The invention relates to a nematicidal composition and application thereof in preventing and controlling nematode infection and development on crops, belonging to the field of agricultural nematicidal compositions. The effective components of the nematicide composition provided by the invention are compounded by fluopyram and hymexazol, and the weight ratio of the fluopyram to the hymexazol is 1:80-80:1, preferably 1:20-20:1. The preparation prepared from the compound has high activity on root knot nematodes; not a simple superposition of the activities of the two components, but a remarkable synergy; the field dosage of the active ingredients is reduced, the production and use cost is reduced, and the pesticide residue and the environmental pollution are reduced; the pesticide consists of active ingredients with different action mechanisms, and the action mechanisms complement each other, thereby being beneficial to overcoming and delaying the generation of pesticide resistance of pests and being safe for crops.

Description

Nematicidal composition and application thereof in preventing and controlling nematode infection and development on crops

Technical Field

The invention belongs to the field of agricultural nematicidal compositions, and particularly relates to a nematicidal composition and application thereof in preventing and controlling nematode infection and development on crops.

Background

Root-knot nematodes are common soil-borne pathogens, and can be parasitic in plant roots to directly harm the plant roots, and the wound caused by the root-knot nematodes can cause secondary infection of other fungi, bacteria and other microorganisms, aggravate soil-borne diseases, cause tumor-like protrusions of the root of the damaged plant, reduce fibrous roots, further cause slow growth of overground parts, dwarf and yellow plants, and cause growth arrest and even death in serious cases. However, root-knot nematodes have become one of the most difficult pests to control in current crop production due to their small size, fast breeding speed, difficult diagnosis, few control agents, strong resistance to the old products, etc. In order to control root knot nematodes, various pesticides are currently available in the market. However, the conventional pesticides have the problems of low safety, large dosage, environmental pollution, serious resistance, poor effect and the like, and always plague pesticide enterprises and farmers. Therefore, how to effectively prevent and control the root-knot nematodes and simultaneously ensure the quality and safety of agricultural products is an urgent problem to be solved.

The fluopyram is a pyridine ethyl benzamide bactericide which is discovered and developed by Bayer and is reported for the first time in 2009, is a succinic dehydrogenase inhibitor (SDHI), has low toxicity in sterilization, high safety and environmental friendliness, and can effectively inhibit fungal spore germination, bud tube elongation, hypha growth and spore formation. In the plant body, fluopyram can be conducted and transferred in xylem, can be used for each growth stage of plants, and has plant surface activity; the systemic conduction effect is provided, so that the systemic conduction effect can be quickly distributed in the whole plant, and the new tissue is protected; it also has thin penetrability, and can prevent and treat diseases on the back even if the front of the leaf is applied with medicine.

The hymexazol belongs to a new generation of novel pesticide bactericides, has systemic property and can be used as a soil disinfectant. Green, environment-friendly, low-toxicity and pollution-free. The hymexazol is absorbed by the soil after entering the soil and is combined with inorganic metal salt ions such as iron, aluminum and the like in the soil, so that the germination of spores and the normal growth of pathogenic fungus mycelia are effectively inhibited or the pathogenic fungi are directly killed, and the efficacy can reach two weeks. Can effectively inhibit the normal growth of pathogenic fungus mycelium or directly kill germs, and has special effects on fusarium wilt, verticillium wilt, damping-off, banded sclerotial blight, leaf blight and continuous cropping obstacle. Meanwhile, hymexazol is also a plant growth regulator, can be absorbed by roots of plants and moves in the roots, and is metabolized to produce two kinds of glycoside, and has the effect of improving physiological activity on crops, so that plant growth, tillering of the roots, increase of root hairs and improvement of the activity of the roots can be promoted. The microbial agent has little influence on bacteria and actinomycetes except pathogenic bacteria in the soil, so that the microbial agent has no influence on the ecology of microorganisms in the soil, can be decomposed into compounds with low toxicity in the soil, and is safe to the environment.

However, there is no report on the application of hymexazol in root-knot nematode control. In addition, the nematicidal composition variety containing a single active component often has defects of different degrees in the agricultural nematicidal composition control, and if different components with synergistic effect are reasonably compounded, the purpose of effectively controlling crop pests can be achieved. The pesticide compound can expand the insecticidal spectrum, improve the control effect, expand the control range, reduce the dosage of active ingredients, save the medication cost and reduce the environmental pollution, so that the research and development of the efficient low-toxicity nematicide composition has positive effects on sustainable development of agriculture.

Disclosure of Invention

The invention aims to provide a nematicidal composition and application thereof in preventing and controlling nematode infection and development on crops, and the nematicidal composition has the advantages of obvious synergistic effect, low cost, good efficacy, long duration and little environmental pollution.

In order to achieve the technical effects, the invention provides a nematicidal composition, which comprises active ingredients of fluopyram and hymexazol in a weight ratio of 1:80-80:1, preferably 1:20-20:1.

Preferably, in the nematicidal composition, the active ingredients fluopyram and hymexazol account for 1 to 90 percent by weight, preferably 10 to 60 percent by weight of the nematicidal composition.

The content of active ingredient in the composition of the present invention depends on the amount applied when used alone, and also on the mixing ratio of one compound to another and the degree of synergism, and is also related to the target disease. The weight percentage of active ingredient in the composition is generally 1% to 90%, preferably 5% to 80% of the total weight. The range of active ingredient content varies according to the type of formulation. Typically, the liquid formulation contains from 1% to 60% by weight of active substance, preferably from 5% to 50%; the solid preparation contains 5 to 80% by weight of active substance, preferably 10 to 80%.

Preferably, the nematicidal composition comprises agricultural preparation auxiliary ingredients besides fluopyram and hymexazol. The auxiliary components of the pesticide preparation comprise a carrier, a solvent, a filler and an auxiliary agent.

Preferably, the carrier is at least one selected from clay particles, broken brick particles, sand, plant source carriers and fertilizers;

the solvent is selected from at least one of organic solvents such as solvent oil, azomethylpyrrolidone, acetophenone, cyclohexanone and dimethyl sulfoxide, and plant source environment-friendly solvents such as turpentine, methyl oleate, soybean oil, corn oil and rapeseed oil;

the filler is at least one selected from kaolin, diatomite, bentonite, attapulgite, white carbon black, starch and light calcium carbonate;

the auxiliary agent comprises at least one surfactant to facilitate the dispersion of the active ingredient in water during application, wherein the surfactant is at least one selected from emulsifying agent, dispersing agent, wetting agent and penetrating agent, the content of the surfactant is 2-30% of the total weight of the preparation, and the balance is solid or liquid diluent. It will be appreciated that the above surfactants are single or complex formulations of conventional nonionic or anionic surfactants.

Preferably, the auxiliary agent can also comprise other functional auxiliary agents selected from one or more of antifreezing agents, thickening agents, stabilizing agents, disintegrating agents, defoaming agents, warning colors and film forming agents according to different application occasions and requirements.

The emulsifier is selected from one or more of Nongru 500# (calcium dodecyl benzene sulfonate), tristyrylphenol polyoxyethylene ether, OP series phosphate (nonylphenol polyoxyethylene ether phosphate), 600# phosphate (phenylphenol polyoxyethylene ether phosphate), styrene polyoxyethylene ether ammonium sulfate salt, alkyl diphenyl ether disulfonate magnesium salt, triethanolamine salt, nongru 400# (benzyl dimethyl phenol polyoxyethylene ether), nongru 700# (alkylphenol formaldehyde resin polyoxyethylene ether), ningru 36# (phenethyl phenol formaldehyde resin polyoxyethylene ether), nongru 1600# (phenethyl phenol polyoxyethylene polypropylene ether), ethylene oxide-propylene oxide block copolymer, OP series (nonylphenol polyoxyethylene ether), BY series (castor oil polyoxyethylene ether), nongru 33# (alkylaryl polyoxyethylene polyoxypropylene ether), span series (sorbitol monostearate), tween series (sorbitol fatty acid ester polyoxyethylene ether) or AEO series (fatty alcohol polyoxyethylene ether).

The dispersing agent is selected from one or more of polycarboxylate, lignosulfonate, alkylphenol ethoxylate formaldehyde condensate sulfate, calcium alkylbenzenesulfonate, naphthalene sulfonate formaldehyde condensate sodium salt, alkylphenol ethoxylate, fatty amine ethoxylate, fatty acid ethoxylate or glycerin fatty acid ester ethoxylate.

The wetting agent is selected from one or more of sodium dodecyl sulfate, sodium dodecyl benzene sulfonate, nekal BX, wetting penetrating agent F, fructus Gleditsiae Abnormalis powder, faeces Bombycis or soapberry powder.

The penetrating agent is selected from one or more of penetrating agent JFC (fatty alcohol polyoxyethylene ether), penetrating agent T (diisooctyl maleate sulfonate), azone or organic silicon.

The antifreezing agent is selected from one or a mixture of more of ethylene glycol, propylene glycol, glycerol or urea.

The thickener is selected from one or more of gelatin, sodium carboxymethyl cellulose, hydroxyethyl cellulose, sodium polyacrylate, modified starch, xanthan gum, bentonite, silicon dioxide or aluminum magnesium silicate.

The stabilizer is selected from one or more of epoxidized soybean oil, epichlorohydrin, BHT, ethyl acetate or triphenyl phosphate.

The disintegrating agent is selected from one or more of bentonite, urea, ammonium sulfate, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid or sodium bicarbonate.

The defoamer is selected from one or a mixture of more of silicone oil, silicone compounds, C10-C20 saturated fatty acid compounds or C8-C10 fatty alcohol compounds.

The warning color is selected from pigment or dye, including natural or synthetic pigment, such as scarlet, rose bengal, fruit green, malachite green, alizarin red, phthalocyanine blue, titanium white, lead chrome yellow, iron blue, etc.

The film forming agent is one or a mixture of a plurality of protein film forming agents, acrylic resin film forming agents, butadiene resin film forming agents, polyurethane film forming agents, nitrocellulose film forming agents, acrylic resin modified casein film forming agents, acrylic resin polyurethane copolymer resins, polyethylene glycol, acrylic ester modified butadiene resins, polyurethane modified nitrocellulose film forming agents and the like.

All of the above materials are commercially available.

The invention also provides an agricultural compound nematicide, which is prepared from the nematicide composition according to any one of the technical schemes.

Preferably, the agricultural compound nematicide can be processed into any pesticide acceptable dosage form according to the requirement. Wherein the preferable dosage forms are wettable powder, water dispersible granule, suspending agent, microemulsion, aqueous emulsion, dispersible oil suspending agent, seed treatment dispersible powder and seed treatment suspending agent.

The composition comprises the following components in percentage by weight when being prepared into wettable powder:

0.01 to 80 percent of fluopyram, 0.01 to 80 percent of hymexazol, 0.01 to 8 percent of wetting agent, 0.01 to 12 percent of dispersing agent, 0.01 to 10 percent of functional auxiliary agent and 100 percent of filler.

The composition is prepared into seed treatment dispersible powder and comprises the following components in percentage by weight:

0.01-80% of fluopyram, 0.01-80% of hymexazol, 0.01-8% of wetting agent, 0.01-12% of dispersing agent, 0.01-10% of defoaming agent, 0.01-20% of warning color, 0.01-20% of film forming agent and 100% of filler.

The composition comprises the following components in percentage by weight when being prepared into water dispersible granules:

0.01-80% of fluopyram, 0.01-80% of hymexazol, 0.01-8% of wetting agent, 0.01-12% of dispersing agent, 0.01-1% of defoaming agent, 0.01-20% of disintegrating agent, 0-20% of binding agent and 100% of filler.

The composition comprises the following components in percentage by weight when being prepared into a suspending agent:

0.01-50% of fluopyram, 0.01-50% of hymexazol, 0.01-10% of wetting agent, 0.01-12% of dispersing agent, 0.01-1% of defoaming agent, 0.01-5% of thickening agent, 0.01-10% of antifreezing agent, 0.01-10% of pH regulator and 100% of water.

The composition is prepared into a seed treatment suspending agent and comprises the following components in percentage by weight:

0.01-50% of fluopyram, 0.01-50% of hymexazol, 0.01-10% of wetting agent, 0.01-12% of dispersing agent, 0.01-1% of defoaming agent, 0.01-5% of thickening agent, 0.01-10% of antifreezing agent, 0.01-10% of pH regulator, 0.01-20% of warning color, 0.01-20% of film forming agent and 100% of water.

The composition is prepared into a suspoemulsion and comprises the following components in percentage by weight:

0.01-50% of fluopyram, 0.01-50% of hymexazol, 0.01-20% of solvent, 0.01-10% of wetting agent, 0.01-20% of emulsifying agent, 0.01-12% of dispersing agent, 0.01-1% of defoaming agent, 0.01-5% of thickening agent, 0.01-10% of antifreezing agent, 0.01-10% of pH regulator and 100% of water.

The composition is prepared into a soluble agent and comprises the following components in percentage by weight:

0.01 to 50 percent of fluopyram, 0.01 to 50 percent of hymexazol, 0.01 to 50 percent of solvent, 0.01 to 20 percent of emulsifier, 0.01 to 20 percent of wetting agent, 0.01 to 1 percent of defoamer, 0.01 to 10 percent of antifreezing agent, 0.01 to 10 percent of pH regulator and 100 percent of water.

The composition is prepared into an aqueous emulsion and comprises the following components in percentage by weight:

0.01 to 50 percent of fluopyram, 0.01 to 50 percent of hymexazol, 1 to 30 percent of solvent, 0.01 to 20 percent of emulsifier, 0.01 to 2 percent of thickener, 0.01 to 1 percent of defoamer, 0.01 to 10 percent of antifreezing agent, 0.01 to 10 percent of pH regulator and 100 percent of water.

The composition is prepared into microemulsion and comprises the following components in percentage by weight:

0.01-50% of fluopyram, 0.01-50% of hymexazol, 0.01-50% of solvent, 0.01-20% of emulsifier, 0.01-1% of defoamer, 0.01-10% of antifreezing agent, 0.01-10% of pH regulator and 100% of water.

The composition is prepared into a dispersible oil suspending agent and comprises the following components in parts by weight:

0.01 to 50 percent of fluopyram, 0.01 to 50 percent of hymexazol, 0.01 to 5 percent of dispersing agent, 0.01 to 30 percent of emulsifying agent, 0.01 to 1 percent of defoaming agent, 0.01 to 10 percent of thickening agent, 0.01 to 10 percent of pH regulator and 100 percent of solvent (continuous phase).

The composition is prepared into granules and comprises the following components in percentage by weight:

0.01 to 50 percent of fluopyram, 0.01 to 50 percent of hymexazol, 0 to 5 percent of wetting agent, 0 to 10 percent of dispersing agent, 0 to 20 percent of emulsifying agent, 0 to 50 percent of solvent (or oil substances such as methyl oleate), 0.01 to 10 percent of pH regulator and 100 percent of carrier.

The invention also provides an application of the nematicidal composition according to any one of the technical schemes in preventing and controlling nematode infection and development on crops.

Preferably, the crop is at least one selected from vegetables, cotton, fruit trees, corn, tobacco, flowers, melons and beans;

the nematode is at least one of root-knot nematode, perforin nematode, bursaphelenchus, swordate nematode, cyclonematode and cyst nematode.

Preferably, the plant growth regulator is used for preventing and controlling the infection and development of root knot nematodes on potatoes, tomatoes, peppers and cucumbers.

The main technical indexes of various dosage forms of the invention meet the related standard requirements of 'specification and formulation of pesticide registration products'.

The invention has the beneficial effects that:

the nematicidal composition provided by the invention is mainly used for crops such as vegetables, cotton, fruit trees, corns, tobaccos, flowers, melons, beans and the like, can be used for preventing and treating various nematodes such as root knot nematodes, perforin nematodes, bursaphelenchus, caenorhabditis elegans, ring nematodes and cyst nematodes, and can be used for effectively preventing and treating various leaf diseases and root diseases, promoting the regulation of root growth, promoting the growth of crop plants, remarkably reducing the application dosage of each active ingredient, reducing the planting cost of crops, reducing pollution and improving the income of farmers.

The concrete steps are as follows:

1. the new function of the product is utilized, and the problem of nematode resistance commonly existing in agricultural production is effectively solved;

2. the synergy is obvious, and the activity of each component is not simply overlapped, but the synergy is obvious;

3. the prevention and treatment effect is strong, the duration is long, and the resistance risk is small;

4. the sterilizing, insecticidal and growth promoting functions, multiple functions, one medicine and multiple treatments, the medicine use times and the medicine use cost are reduced, the income of farmers is improved, and the pesticide residues and the environmental pollution are reduced;

5. the composition consists of active ingredients with different action mechanisms, has complementary actions, is beneficial to overcoming and delaying the generation of drug resistance of pests, and is safe to crops.

Detailed Description

The following description of the technical solutions in the embodiments of the present invention will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

And combining fluopyram and hymexazol according to different proportions, and detecting the control effect on the meloidogyne incognita. The test method is as follows:

the embodiment of the invention adopts a method combining indoor toxicity measurement and field test. Firstly, determining the co-toxicity coefficient (CTC) of two medicaments compounded according to a certain proportion through indoor toxicity measurement, wherein CTC is smaller than 80 and is antagonistic, CTC is smaller than or equal to 80 and is smaller than or equal to 120 and is additive, CTC is larger than 120 and is synergistic, and then performing field test on the basis.

The test method comprises the following steps: in the test, the water suspension agent mother liquor of each mixed agent is diluted into five series of concentrations and placed in a beaker for standby.

Culturing the meloidogyne incognita: taking potato root systems with a large number of root knots, gently washing the potato root systems with water, gently picking milky oocysts on the diseased root systems with an dissecting needle, placing the milky oocysts into a small culture dish with the diameter of 6cm, adding a small amount of sterile water, incubating for 3-4 days in a constant temperature box with the temperature of 25 ℃, collecting second-age larvae by a whole-process reference creep arming method, adding the sterile water, and preparing a suspension with a certain concentration (about 1000 strips/mL) for later use.

The method of 96 plate holes is adopted, a continuous sample feeder is used for adding nematode suspension into the plate holes, 30 microliters of the nematode suspension are added into each hole, then different doses of liquid medicine are respectively added according to the sequence from low to high, 5 different concentration gradients are added, the mixture is uniformly shaken, and the mixture is placed in a constant temperature incubator at 25 ℃, the humidity is kept at 60-80%, and the observation is carried out. 3 replicates were set for each treatment, with fresh water control, stiff nematodes were dead, curved nematodes were alive, dead rates were observed at 24h, 48h, 72h, mortality and corrected mortality were calculated, finney probability value analysis was referenced, corrected mortality of pests was converted to a probability value (y), treatment concentration (μg/ml) was converted to a number (x), data treatment was performed with IBM SPSS Statistics statistical software, a virulence regression equation was developed, and LC50 values for each agent were calculated therefrom. If the control mortality rate is greater than 10%, it is considered an ineffective test. The calculation formula is as follows:

mortality (%) = ((number of live insects before drug-number of live insects after drug)/number of live insects before drug) ×100%

Mortality correction rate (%) = ((treatment group mortality-control group mortality)/(100-control mortality)) ×100%

The co-toxicity coefficient CTC was calculated according to the Sun Yun Pei formula. The calculation formula is as follows (fluopyram is used as a standard medicament, and the toxicity index is 100):

toxicity index of hymexazol (TI) = (LC 50 of fluopyram/LC 50 of hymexazol) ×100

Actual virulence index of M (ATI) = (LC 50 of fluopyram LC50/M LC 50) ×100

Theoretical virulence index (TTI) =ti×p of fluopyram for M _Fluopicolide TI x P of +effective hymexazol _Hymexazol

Co-toxicity coefficient (CTC) = (ATI/TTI of M) ×100

Wherein: m is a mixture with different proportions

P _Fluopicolide Is the proportion of fluopyram in the composition

P _Hymexazol Is the proportion of hymexazol in the composition

The toxicity measurement results are shown in Table 1, and the synergy measurement results are shown in Table 2. Wherein, CK1 is fluopyram single agent and CK2 is hymexazol single agent.

TABLE 1 toxicity determination results of fluopyram and hymexazol on potato root knot nematode in different ratios

Table 2 results of synergy measurements of fluopyram and hymexazol in different ratios on potato root knot nematodes

As can be seen from table 1 and table 2, the co-toxicity coefficients of fluopyram and hymexazol composition are both greater than 120, and the synergistic effect is shown, and the weight ratio is 1:2, 1:3:1:5: at 1:8, the co-toxicity coefficient is much higher than 120 times, indicating that at 1:2-1: the synergy is most obvious at 8. The application weight ratio is illustrated as 10:1 to 1: the compound preparation of the fluopyram and the hymexazol has reasonability and feasibility for preventing and controlling the potato root knot nematode.

The control effect of the fluopyram on tomatoes, peppers and cucumber root-knot nematodes is measured by the method, and the synergy coefficients in different proportioning ranges are calculated, so that the fluopyram and hymexazol are combined to show a synergy effect: 1:2, 1:3:1:5:1:8 the most obvious conclusion of synergy.

The present invention will be specifically described with reference to examples. In the examples, the percentages are weight percentages, but the invention is not limited to the percentages, and the treatment dosage of the field efficacy is the dosage of the effective components.

The wettable powder is as follows:

example 1: (40% fluopyram-hymexazol wettable powder) 1:3

The components and the content are as follows: fluopyram: 10%; hymexazol: 30%; wetting agent sodium dodecyl sulfate: 5%; dispersant calcium lignosulfonate: 8%; dispersant naphthalene sulfonate: 4%; the filler is white carbon black: 5%; defoaming agent: 0.2%; kaolin: the balance.

Example 2: (70% fluopyram-hymexazol wettable powder) 1:6

The components and the content are as follows: fluopyram: 10%; hymexazol: 60 percent; wetting agent sodium dodecyl benzene sulfonate: 3%; dispersant calcium alkyl benzene sulfonate: 7%; dispersant naphthalene sulfonate: 3%; the filler is white carbon black: 5%; defoaming agent: 0.2%; light calcium carbonate: the balance.

Example 3: (11% fluopyram-hymexazol wettable powder) 1:0.1

The components and the content are as follows: fluopyram: 10%; hymexazol: 1%; the wetting agent is nekal BX: sodium dodecyl benzene sulfonate and wetting penetrating agent F in the amount of 1%, 1% and 1% respectively; the dispersant is polycarboxylate and naphthalene sulfonate, and the dosage is 1% and 3% respectively; white carbon black: 3%; defoaming agent: 0.2%; the filler is diatomite: 20%, kaolin: the balance.

The preparation methods of the above examples 1 to 3 are: according to the components and the weight percentages thereof provided by the embodiment, the hymexazol and the fluopyram which are active components are added into the filler, and the auxiliary agents such as wetting agent, dispersing agent and the like are added into the filler, and the mixture is uniformly mixed, and then the mixture is mixed after jet milling to prepare the wettable powder. The main equipment is a mixer and an air flow pulverizer.

In the above examples, each index of the preparation samples meets the requirements in the wettable powder management specification.

The water dispersible granule comprises the following components:

example 4: (30% fluopyram and hymexazol water dispersible granule) 1:2

The components and the content are as follows: fluopyram: 10%; hymexazol: 20% of a base; wetting agent sodium dodecyl benzene sulfonate: 3%; dispersant alkyl naphthalene sulfonate formaldehyde condensate: 8%; defoaming agent: 0.2%; filling attapulgite: the balance.

Example 5: (42% fluopyram-hymexazol water dispersible granule) 1:5

The components and the content are as follows: fluopyram: 7%; hymexazol: 35%; wetting agent gleditsia sinensis powder: 3%; dispersant naphthalene sulfonic acid formaldehyde condensate sodium salt: 2%; dispersing agent polycarboxylate: 2%; defoaming agent: 0.2%; and the balance of the filling attapulgite.

The preparation methods of the above examples 4 to 5 are: according to the components and the weight percentages thereof provided by the embodiment, the effective components of hymexazol and fluopyram are added into the filler, and auxiliary agents such as wetting agents, dispersing agents and the like are added into the filler for mixing, 10 to 25 percent of water is added after air current crushing, and then the water dispersible granule product is prepared through kneading, granulating, drying and screening; or spraying water, granulating, drying, and sieving to obtain the final product. The main equipment comprises a mixer: jet mill, kneader, extrusion granulator, drying in drying room or fluidized bed, or boiling granulator, sieving machine.

In the embodiment, the preparation samples have all indexes meeting the requirements in the water dispersible granule management standard.

The suspending agent is as follows:

example 6: (27% fluopyram-hymexazol suspension) 1:8

The components and the content are as follows: fluopyram: 3%; hymexazol: 24%; wetting agent NP-10:2%; dispersing agent polycarboxylate: 2%; dispersant phosphate: 2%; the thickener is xanthan gum and magnesium aluminum silicate, and the dosage is 0.1% and 1% respectively; antifreeze glycol: 5%; defoamer silicone-based compounds: 0.2%; water, balance.

Example 7: (5.5% fluopyram-hymexazol suspension) 1:10

The components and the content are as follows: fluopyram: 0.5%; hymexazol: 5%; wetting agent NP-10:1%; dispersant alkylphenol ethoxylates: 3%; dispersing agent polycarboxylate: 2%; the thickener is xanthan gum and magnesium aluminum silicate, and the dosage is 0.15% and 2% respectively; antifreeze glycol: 5%; defoaming agent silicone oil: 0.2%; water: the balance.

Comparative example 1: (30% hymexazol suspension)

The components and the content are as follows: : hymexazol 30%; wetting agent sodium dodecyl sulfate: 2%; dispersing agent polycarboxylate: 2%; dispersant phosphate: 3%; dispersant alkylphenol ethoxylates: 2%; the thickener is xanthan gum and magnesium aluminum silicate, and the dosage is 0.15% and 1.5% respectively; antifreeze glycol: 5%; defoaming agent silicone oil: 0.2%; water: the balance.

Comparative example 2: (30% fluopyram suspension)

The components and the content are as follows: fluopyram: 30%; wetting agent sodium dodecyl sulfate: 2%; dispersing agent polycarboxylate: 2%; dispersant phosphate: 3%; dispersant high molecular polymer: 2%; the thickener is xanthan gum and magnesium aluminum silicate, and the dosage is 0.1% and 1% respectively; antifreeze glycol: 5%; defoaming agent silicone oil: 0.2%; water: the balance.

The preparation methods of examples 6 to 7 and comparative examples 1 and 2 are as follows: according to the components and the weight percentages thereof provided by the embodiment, the effective components of hymexazol, fluopyram, wetting agent, dispersing agent, thickening agent, antifreezing agent, defoaming agent and other auxiliary agents are sequentially placed in a reaction kettle, water is added and mixed uniformly, and the products are obtained after high-speed shearing, wet sanding and uniform filtration. The main equipment is a batching kettle, a colloid mill or a homogenizing mixer and a sand mill.

Each index of the preparation samples of the above examples and comparative examples meets the requirements in the suspending agent management specification.

The dispersible oil suspending agent is as follows:

example 8: (30% fluopyram-hymexazol dispersible oil suspension) 1:2

The components and the content are as follows: fluopyram: 10%, hymexazol: 10% of dodecylbenzene sulfonic acid calcium salt: 5%, castor oil polyoxyethylene ether: 1% of fatty alcohol polyoxyethylene ether: 8, selecting organic soil and gas-phase white carbon black as thickening agents, wherein the organic soil and the gas-phase white carbon black are respectively 0.7%; soybean oil balance.

Example 9: (35% fluopyram-hymexazol dispersible oil suspension) 2:3

The components and the content are as follows: fluopyram: 15%, hymexazol: 20%, calcium dodecylbenzenesulfonate salt: 4%, castor oil polyoxyethylene ether: 2%, fatty alcohol polyoxyethylene ether: 10%, isomerised alcohol ether: 2, selecting organic soil and gas-phase white carbon black as a thickener, wherein the organic soil and the gas-phase white carbon black are respectively 0.6%; soybean oil balance.

The preparation methods of the above examples 8 to 9 are: according to the components and the weight percentages thereof provided by the embodiment, the soybean oil, the effective components hymexazol, fluopyram, an emulsifying agent, a dispersing agent, a thickening agent, a defoaming agent and other auxiliary agents are sequentially placed in a reaction kettle, uniformly mixed, sheared at a high speed, sanded by a wet method, and finally filtered uniformly to obtain the product. The main equipment is a batching kettle, a colloid mill or a homogenizing mixer and a sand mill.

In the above examples, each index of the formulation samples meets the requirements in the dispersible oil suspension agent management specification.

The effect of the present invention was more fully and accurately demonstrated by performing a field drip irrigation test on potato root knot nematodes using the formulations of examples 1, 4, 5, 6, 8 and comparative examples 1 and 2 at the dilution factors shown in table 3, and the test sites were selected from the field test for Qingdao Han Biotechnology Co., ltd. In Qingdao Laiyi, and the soil conditions, water fertilizers and various management were consistent during the test. The test results are shown in table 3 below. Wherein:

example 1:40% fluopyram-hymexazol wettable powder (10+30);

example 4:30% fluopyram and hymexazol water dispersible granule (10+20);

example 5:42% fluopyram-hymexazol water dispersible granule (7+35);

example 6:27% fluopyram-hymexazol aqueous suspension (3+24);

example 8:30% fluopyram-hymexazol dispersible oil suspension (10+20);

comparative example 1:30% of hymexazol aqueous suspension;

comparative example 2:30% fluopyram aqueous suspension.

In this test, the agents of examples 1, 4, 5, 6, 8 were: the medicines of comparative examples 1 and 2 and the medicine without any use are 8 treatments, each treatment is repeated 3 times, 24 treatment cells are provided, each cell is 30 square meters, isolation rows are arranged among the cells, and the treatments of the cells are arranged randomly. The dosage of each plant of active ingredient is 0.01g, the diluent is 400ml, and drip irrigation is implemented at the initial stage of the onset of the potato root knot nematode disease. 3 points are randomly taken from each treatment district, 3 strains of potatoes are taken from each point, root knot disease conditions of potato roots are respectively investigated 1 day, 3 days and 7 days after the administration, and grading standards of the root knot nematode disease are as follows:

grade 0 (root system without visible root knot);

level 1 (1% -10% root system has root knot);

level 2 (10% -25% root system with root knot);

grade 3 (25% -50% root system with root knot);

level 4 (50% -80% root system has root knot);

grade 5 (root system with root knot above 80%).

The capability of preventing and controlling the root-knot nematode is based on two indexes of disease index and preventing and controlling effect of the root-knot nematode, and simultaneously, the growth condition of potatoes in each district is observed and recorded: and the occurrence of phytotoxicity.

The disease index and the prevention and treatment effect calculating method comprises the following steps:

disease index (%) = (Σ number of disease stage disease plants×number of disease stage)/(total number of investigation×highest stage) ×100%

Control effect (%) = (control disease index-treatment disease index)/control disease index×100%

TABLE 3 control effects of different treatments on potato root knot nematode disease

As can be seen from the test results in Table 3, the effect of the medicaments in examples 1, 4, 5, 6 and 8 on controlling potato root-knot nematodes is obviously higher than that of the single medicaments of fluopyram and hymexazol in comparative examples 1-2 after 1, 3 and 7 days of administration. And the medicament has long lasting time, and still achieves good control effect after 7 days of medicament application. The preparation with the same proportion and the same content is also illustrated by the table 3, the efficacy of the dispersible oil suspending agent is higher than that of the water dispersible granule, and the effect of the oil in the preparation on the preparation is verified to be promoted. The experiment period shows that the medicines of the embodiments have no phytotoxicity to potatoes and good safety.

In addition, referring to the above treatment method, a biological test was performed on three crops of tomato, pepper and cucumber using 30% fluopyram and hymexazol OD (1:2), the dosage of the active ingredient per plant was 0.01g, and drip irrigation was performed with 400ml of the diluted solution, and the test data are shown in Table 4.

TABLE 4 control effect of 30% fluopyram-hymexazol OD on root knot nematode disease of tomato, capsicum and cucumber

Through table 4, it is further verified that the fluopyram and hymexazol have better control effects on root-knot nematodes of different crops of tomatoes, peppers and cucumbers after being compounded, the control effect is highest on the 3 rd day, the control capability is still higher on the 7 th day, the duration of effect is long, and the crops are normally produced during the administration period without obvious phytotoxicity.

With the agent, 0.01g, 0.012g and 0.015g of effective components are respectively used for cucumber root-knot nematodes, and 400m of diluent is used for drip irrigation, so that the control effect of different dosage on the root-knot nematodes is further verified, and test data are shown in Table 5.

TABLE 5 control effect of different 30% fluopyram hymexazol OD dosages on cucumber root knot nematode disease

As can be seen from Table 5, with the increase of the dosage, the control effect of the 30% fluopyram-hymexazol OD on the cucumber root knot nematode disease also shows an increasing trend, the peak is reached on the 3 rd day, the higher control effect is still achieved on the 7 th day, the lasting period is long, and the cucumber grows normally in the whole dosage period without obvious phytotoxicity.

The results fully show that the fluopyram and the hymexazol have the advantages of obvious synergism and prolonged drug effect on root-knot nematodes after being compounded, and the whole process is safe.

Finally, in the invention, the fluopyram and the hymexazol are combined to find that the root-knot nematode is slow to start moving after being dosed, and the symptoms are initially developed immediately after the treatment, so that the root-knot nematode becomes completely paralyzed and immobilized for 1 h. The effect is obviously better than that of two single agents, one component, and the composition is slowly and uniformly distributed in the soil on the rhizosphere soil layer, so that the plant root system can be effectively and long-term protected from nematode invasion. The compound combination of the two can be used for preventing and controlling various nematodes such as root-knot nematodes, perforin-parvula, burley nematodes, caenorhabditis elegans, and cycloparaffins, and has good prevention effect on cyst nematodes. Because both the two active ingredients have systemic property, the soil application does not influence the control of various leaf diseases and root diseases and the promotion of root growth regulation, and even can control nematodes on the ground. And is suitable for various application methods (drip irrigation, root irrigation, ditch application, soil mixed application and the like). The two components are compounded to have the functions of sterilization, disinsection and growth promotion, and after the technology is converted and produced, the medication cost is obviously reduced, the prevention effect is improved, the dosage is reduced, the environmental pollution is reduced, and the value created for enterprises and farmers is considerable.

Claims (10)

1. The nematicidal composition is characterized in that the active ingredients of the nematicidal composition are composed of fluopyram and hymexazol in a weight ratio of 1:80-80:1, preferably 1:20-20:1.

2. A nematicidal composition according to claim 1 wherein the active ingredients fluopyram and hymexazol comprise from 1% to 90%, preferably from 10% to 60% by weight of the nematicidal composition.

3. The nematicidal composition of claim 1 or 2 further comprising a pesticide formulation adjunct ingredient comprising a carrier, a solvent, a filler, and an adjuvant.

4. A nematicidal composition according to claim 3 wherein the carrier is selected from at least one of clay particles, crushed brick particles, sand, plant-derived carriers, fertilisers;

the solvent is selected from at least one of organic solvents such as solvent oil, azomethylpyrrolidone, acetophenone, cyclohexanone and dimethyl sulfoxide, and plant source environment-friendly solvents such as turpentine, methyl oleate, soybean oil, corn oil and rapeseed oil;

the filler is at least one selected from kaolin, diatomite, bentonite, attapulgite, white carbon black, starch and light calcium carbonate;

the auxiliary agent comprises at least one surfactant, and the surfactant is at least one selected from emulsifying agents, dispersing agents, wetting agents and penetrating agents.

5. The nematicidal composition of claim 4 wherein said adjuvants further comprise other functional adjuvants selected from at least one of antifreeze agents, thickening agents, stabilizers, disintegrants or defoamers, alert colors, film formers.

6. An agricultural compound nematicide, characterized in that it is prepared by using the nematicide composition of any one of claims 1 to 5.

7. The agricultural compound nematicide of claim 6 wherein the dosage form is at least one selected from the group consisting of wettable powders, water dispersible granules, dry suspensions, dispersible oil suspensions, seed treatment dispersible powders, solutions, emulsifiable concentrates, aqueous suspensions, aqueous emulsions, microemulsions, suspoemulsions, and granules.

8. Use of a nematicidal composition according to any one of claims 1 to 5 for controlling nematode infestation, development on crops.

9. The use according to claim 8, wherein the crop is at least one selected from the group consisting of vegetables, cotton, fruit trees, corn, tobacco, flowers, melons, beans;

the nematode is at least one of root-knot nematode, perforin nematode, bursaphelenchus, swordate nematode, cyclonematode and cyst nematode.

10. The use according to claim 8, characterized in that it prevents the infestation, development of root-knot nematodes on potatoes, tomatoes, peppers, cucumbers.