CN114176090A - Application of bactericidal composition containing prothioconazole and metalaxyl-M in preventing and treating peanut diseases - Google Patents
Application of bactericidal composition containing prothioconazole and metalaxyl-M in preventing and treating peanut diseases Download PDFInfo
- Publication number
- CN114176090A CN114176090A CN202111558047.6A CN202111558047A CN114176090A CN 114176090 A CN114176090 A CN 114176090A CN 202111558047 A CN202111558047 A CN 202111558047A CN 114176090 A CN114176090 A CN 114176090A
- Authority
- CN
- China
- Prior art keywords
- peanut
- bactericidal composition
- active ingredient
- metalaxyl
- prothioconazole
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention relates to an application of a bactericidal composition containing prothioconazole and metalaxyl-M in controlling peanut diseases; the pesticide composition at least comprises an active ingredient A prothioconazole and an active ingredient B metalaxyl-M, wherein the mass ratio of the active ingredient A prothioconazole to the active ingredient B metalaxyl-M is 1: 50-50: 1. The bactericidal composition can be used for preventing and treating various diseases on peanuts, has good prevention and treatment effects, can prolong the lasting period of a medicament, reduce the medicament application times, effectively reduce the use amount and the use cost of effective components, delay the generation and the development of resistance, and is environment-friendly.
Description
Technical Field
The invention relates to the field of pesticide bactericide application, and in particular relates to application of a bactericidal composition containing prothioconazole and metalaxyl-M in controlling peanut diseases.
Background
Prothioconazole (prothioconazole) belongs to sterol demethylation (ergosterol biosynthesis) inhibitors, has a systemic fungicide with protection, treatment and eradication activity, and has a long duration. The prothioconazole is mainly used for preventing and treating a plurality of diseases of cereals, wheat and bean crops and the like, has low toxicity, no teratogenicity and mutagenicity, has no toxicity to embryos, and is safe to human and environment when being correctly used. CAS registry number 178928-70-6, having the chemical structure:
metalaxyl-M is a systemic aniline compound and has very good control effect on various seed-borne and soil-borne diseases caused by oomycetes fungi such as pythium, cotton and the like. The systemic bactericide absorbed by plant leaves, stems and roots has protective and therapeutic activities. The protein synthesis of fungi is inhibited by blocking ribonucleic acid synthesis. CAS registry number 70630-17-0, having the chemical structure:
peanuts are important oil crops and are planted all over the country. In recent years, with the steady improvement of economic benefits of peanut planting, the planting area is continuously increased, the crop rotation difficulty is caused, the phenomenon of continuous cropping in the whole year is prominent, meanwhile, due to the continuous improvement of the production level, the peanuts grow vigorously, the field ventilation and light transmittance are poor, the humidity is high, a proper ecological environment condition is provided for the occurrence of peanut root and stem diseases, in addition, the reduction of rural labor force, the field management is extensive, weeds grow, the harm of the peanut root and stem diseases is aggravated year by year, and the peanut yield is low, the quality is poor, and the economic benefit is poor.
With the long-term use of a large amount of single selective medicaments, the pathogenic fungi in nature have different degrees of drug resistance, and the control effect declines year by year. Chemical control is one of effective measures for controlling plant soil-borne fungal diseases, and the seed medicament coating is time-saving and labor-saving and is more easily accepted by farmers.
The patent application with the publication number of CN105394048A discloses a sterilization composition containing prothioconazole and efficient metalaxyl-M, wherein the effective components of the pesticide composition, namely prothioconazole and efficient metalaxyl-M, are compounded in a binary way, so that diseases such as gray mold, powdery mildew, rust disease and the like on vegetables and flowers and fruits can be effectively controlled. With the increasing requirements on environment and food safety and the resistance problem of medicaments, how to scientifically apply the medicaments, reduce the dosage of chemical pesticides and improve the pesticide effect becomes a problem which is urgently needed to be solved in the field of pesticides; in addition, no related report exists on the application of the bactericidal composition of prothioconazole and metalaxyl-M in controlling peanut diseases. The environmental protection concept of the broad farmers is more and more deeply known, and the inevitable trend of pesticide development is that the pesticide has high efficiency, low toxicity, high activity and low residue.
Disclosure of Invention
Based on the situation, the invention aims to provide the application of the bactericidal composition containing prothioconazole and metalaxyl-M in controlling peanut diseases, the bactericidal composition can be used for controlling various diseases on peanuts, is good in quick-acting performance, and can delay the development of the drug resistance of the peanut diseases.
In order to achieve the purpose, the bactericidal composition containing prothioconazole and metalaxyl-M is used for preventing and treating peanut diseases, the bactericidal composition at least comprises an active ingredient A and an active ingredient B, the active ingredient A is prothioconazole, the active ingredient B is metalaxyl-M, and the mass ratio of the prothioconazole of the active ingredient A to the metalaxyl-M of the active ingredient B is 50: 1-1: 50;
further, the mass ratio of the prothioconazole as the active ingredient A to the metalaxyl-M as the active ingredient B is 10: 1-1: 10;
further, the mass ratio of the prothioconazole as the active ingredient A to the metalaxyl-M as the active ingredient B is 10:1, 7:1, 3:1, 2:1, 5:3, 3:2, 1:1, 3:4, 1:5 and 1: 10;
further, the mass ratio of the active component A prothioconazole to the active component B metalaxyl-M is 7: 1-1: 5;
further, the mass ratio of the active component A prothioconazole to the active component B metalaxyl-M is 3: 1-3: 4;
further, the mass ratio of the prothioconazole as the active ingredient A to the metalaxyl-M as the active ingredient B is 3:1, 2:1, 5:3, 3:2, 1:1 and 3: 4;
furthermore, the sum of the content of the active component A and the active component B in the bactericidal composition is 0.1-80 percent based on the total weight of the bactericidal composition as 100 percent by weight,
further, the sum of the contents of the active ingredient A and the active ingredient B in the bactericidal composition is 2-50% by weight of the total weight of the bactericidal composition being 100 wt%;
further, the sum of the contents of the active ingredient A and the active ingredient B in the bactericidal composition is 2-30% by weight of the total weight of the bactericidal composition being 100 wt%;
further, the bactericidal composition also comprises an auxiliary agent, wherein the auxiliary agent is selected from one or more of a wetting agent, a dispersing agent, an emulsifying agent, a thickening agent, a disintegrating agent, an antifreezing agent, an antifoaming agent, a solvent, a preservative, a stabilizer, a warning color, a film forming agent, a synergistic agent and a carrier;
further, the dosage form of the bactericide composition is any one of agriculturally allowable dosage forms;
furthermore, the dosage form of the bactericidal composition is any one of aqueous solution, wettable powder, microemulsion, aqueous emulsion, suspending agent, missible oil, water dispersible granules, suspended seed coating agent, seed treatment suspending agent, microcapsule suspending agent and dry seed mixing agent;
the wetting agent is selected from one or more of alkyl benzene sulfonate, alkyl naphthalene sulfonate, lignosulfonate, sodium dodecyl sulfate, dioctyl sodium sulfosuccinate, alpha-olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, sodium fatty alcohol polyoxyethylene ether sulfate, silkworm excrement, Chinese honeylocust fruit powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and/or
The dispersing agent is selected from one or more of lignosulfonate, alkyl naphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylate, polyacrylic acid, phosphate, EO-PO block copolymer and EO-PO graft copolymer; and/or
The emulsifier is selected from one or more of calcium dodecylbenzene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethyl phenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styryl phenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and/or
The thickener is one or more selected from xanthan gum, organic bentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and/or
Disintegrant the disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and/or
The antifreezing agent is selected from one or more of alcohols, alcohol ethers, chlorohydrocarbons and inorganic salts; and/or
The defoaming agent is selected from C10-C20Saturated fatty acid compound, silicone oil, silicone compound, C8-C10One or more of fatty alcohols; and/or
The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel oil, solvent oil, vegetable oil derivative and water; and/or
The preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium parahydroxybenzoate, methyl parahydroxybenzoate, carbazone and 1, 2-benzisothiazoline 3-one; and/or
The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2, 6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talcum powder, montmorillonite and starch; and/or
The warning color is selected from any one or more of blue, green, red and purple; and/or
The film forming agent is selected from one or more of carboxymethyl starch sodium, cellulose derivatives (carboxymethyl cellulose sodium, sodium alginate, polyvinyl alcohol), and polyacrylic acid; and/or
Further, the film forming agent is selected from a seed coating film forming agent 851 and a seed coating film forming agent 805; and/or
The synergist is selected from synergistic phosphorus and synergistic ether; and/or
The carrier is selected from one or more of ammonium salt, ground natural mineral, ground artificial mineral, silicate, resin, wax, solid fertilizer, water, organic solvent, mineral oil, vegetable oil and vegetable oil derivative;
further, the dosage form of the bactericidal composition is a seed treatment suspending agent.
The application of a bactericidal composition containing prothioconazole and metalaxyl-M in preventing and treating peanut diseases, wherein the peanut diseases are bacterial diseases or fungal diseases;
further, the Peanut diseases are black spot disease (Cercospora personata), brown spot disease (Cercospora arachidicola), net spot disease (Ascochyta arachididis), anthracnose disease (Colletotrichum truncatum), Peanut focal spot disease (leptosphaeria crassiosa), purple stripe disease (Helicobasidium moma), rust disease (Puccinia arachididis), bacterial wilt disease (Balstonia solanacearun), stem rot disease (dipoda gossypina), southern sclerotinia blight (sclerotinioides rolfsii), root rot disease, Peanut yellow Mosaic Virus (cuumber Mosaic Virus-China ariosis, CMV-CA), stripe disease (peninsula virens, pstvs), dwarf Virus (villius), and Peanut crown rot disease (Aspergillus niger).
Furthermore, the peanut diseases comprise peanut southern blight and peanut root rot;
the pathogen of the peanut southern blight is Sclerotium rolfsii, belonging to fungi imperfecti. Basidiomycetes and basidiospores can be produced, but are not common. The mycelium of the pathogenic bacteria is white, constant roots to dozens of roots are twisted into a cord shape and radially spread, and the appearance of the mycelium is like white silk, so the mycelium is commonly called southern blight. Southern blight of peanuts mainly damages stems, fruit stalks and pods of peanuts, and the diseased parts become brown and soft rot at the early stage of disease, and corrugated disease spots appear on the diseased parts. When the soil is wet and hidden, a layer of white mycelium is also covered on the soil surface plant residues and organic matters around the plant. Many spherical sclerotia are formed in the mycelium. The tissue at the base of the injured stem decays, the cortex falls off, leaving fibrous tissue. The leaves of the diseased plant turn yellow, the edges are scorched and finally wither and die, and a plurality of white hyphae grow on the affected fruit stalks and pods and are rotten in a wet rotten state.
Furthermore, the peanut diseases are peanut root rot;
the peanut root rot is caused by infection of various fusarium and Pythium species, wherein fusarium oxysporum (f.oxysporum) and Pythium graminearum (Pythium mycinotylum) are two main pathogenic bacteria. Wherein the Fusarium app for inducing pathogenic fungi imperfecti of peanut root rot comprises Fusarium oxysporum, Fusarium solani F solani, Fusarium roseum F roseum, Fusarium triplex F tricinctum and Fusarium moniliforme F moniliforme. The root rot of peanut can be attacked in each growth period, and is mainly harmful to the root of the plant. Pathogenic bacteria infect the newly germinated seeds to cause rotten seeds; when the seedling is damaged, the main root turns brown and the plant withers. The adult plants are damaged, the main root and the rhizome have sunken elongated brown lesions, the root end is in a wet rot shape, the cortex is browned and rotten, and the adult plants are easy to fall off, have no lateral roots or few rat tails and are shaped like rat tails. When wet, adventitious roots grow at the root and stem parts. The aerial parts of the diseased plants are short and small, the plants grow badly, the leaves turn yellow, and the flowers and the fruits are few and are blighted fruits.
The application of a bactericidal mixture containing prothioconazole and metalaxyl-M in controlling peanut diseases is characterized in that the bactericidal composition is applied to diseases needing to be controlled or a growth medium thereof in an effective dose.
Compared with the prior art, the technical scheme of the invention has the following beneficial effects:
1) the bactericidal composition can prevent and treat peanut diseases, expand the prevention and treatment spectrum, and save labor and medicament cost;
2) the bactericidal composition shows a certain synergistic effect on the prevention and treatment of peanut diseases in a certain range, can reduce the usage amount of pesticide active ingredients, and improves the prevention and treatment effect;
3) the bactericidal composition can prolong the lasting period of the pesticide, reduce the pesticide application times, delay the generation of the drug resistance of harmful bacteria and prolong the service life of a single pesticide.
Detailed Description
To make the technical solutions, objects, and advantages of the present invention more apparent, the present invention is described with the following specific examples, but the present invention may be implemented in various forms and should not be limited by the embodiments set forth herein.
Indoor bioassay
Examples reference is made to the criteria for indoor bioassay of pesticides, part 2, the plate method for inhibiting the growth of pathogenic fungi hyphae, NY/T1156.2-2006.
Experimental apparatus equipment: electronic balance (induction 0.01mg), clean bench, biological incubator, phi 9cm culture dish, pipette, inoculating loop, puncher, caliper, etc.
Reagent to be tested: 95 percent of prothioconazole and 91 percent of metalaxyl-M are provided by a group research and development center.
Indoor example 1
Indoor toxicity determination method for peanut southern blight
Pathogenic bacteria to be tested: sclerotinia sclerotiorum (Sclerotium rolfsii Sacc) was supplied by the research and development center of the group.
Preparing a medicament: the prothioconazole and metalaxyl-M original drugs are dissolved by acetone and then diluted by 0.1 percent of Tween-80 aqueous solution. Preparing single-dose mother liquor respectively, designing 5 groups of proportioning according to the mixing purpose and the medicament activity, and preparing 5 series of mass concentrations of each single dose and each group of proportioning mixing agent according to an equal ratio method.
Under aseptic operation conditions, according to test treatment, 45ml of a pre-melted sterile culture medium is added into a sterile conical flask, 5ml of liquid medicine is sequentially sucked from low concentration to high concentration, and is respectively added into the conical flasks, fully shaken, and then poured into 3 culture dishes in equal amount to prepare a medicine-containing plate with corresponding concentration. The test was run with no agent treatment as a blank, which was repeated 3 times per treatment.
The cultured pathogenic bacteria are cut from the edge of a bacterial colony by a sterilization puncher with the diameter of 5mm under the aseptic operation condition, the bacterial cake is inoculated to the center of a drug-containing flat plate by an inoculator, the hypha surface is upward, 1 bacterial cake is placed in each culture dish, the dish cover is covered, and the culture dish is placed in an incubator (26 +/-0.5 ℃) for culture.
Data statistics and analysis:
and (5) investigating the growth condition of the hyphae of the pathogenic bacteria according to the growth condition of the bacteria in the blank control culture dish. The colony diameter was measured in millimeters (mm) with a caliper. The diameter of each colony was measured perpendicularly by the cross method once and averaged.
According to the investigation results, the hypha growth inhibition rate of each treatment concentration on the test target bacteria is calculated, and the calculation result in percentage (%) retains two decimal points.
D＝D1-D2
In the formula:
d-colony growing diameter;
D1-colony diameter;
D2-the diameter of the cake.
In the formula:
i-hypha growth inhibition rate;
D0-growing the diameter of the blank colony;
DTmedicament-treated colonies grow in diameter.
And (4) performing regression analysis according to the concentration logarithm value of each medicament and the corresponding probability value of the hypha growth inhibition rate. Finding EC of virulence regression line50The value and the correlation coefficient r.
Sun Yupei method: evaluating the synergistic effect of the mixed medicament according to the co-toxicity coefficient (CTC), wherein the compounded co-toxicity coefficient (CTC) is more than or equal to 120 and shows the synergistic effect; CTC is less than or equal to 80 and shows antagonism; 80 < CTC < 120 showed additive effects.
Co-toxicity coefficient (CTC value) calculation of the blend:
in the formula:
ATI-actually measured toxicity index of mixed agent;
S-EC of Standard Fungicide50In milligrams per liter (mg/L);
EC of M-mixtures50In milligrams per liter (mg/L).
TTI＝TIA*PA+TIB*PB
In the formula:
TTI-mixture theory virulence index;
TIA-agent virulence index a;
PA-the percentage of agent a in the mixture in percent (%);
TIB-agent B virulence index;
PB-the percentage of the agent B in the mixture in percent (%).
In the formula:
CTC-co-toxicity coefficient;
ATI-actually measured toxicity index of mixed agent;
TTI-mixture theory virulence index.
Results and analysis:
the two agents of the prothioconazole and the metalaxyl-M have different action mechanisms, and the compound use is beneficial to overcoming or delaying the development of drug resistance of germs and improving the prevention and treatment effect.
Test results show that as shown in Table 1, the prothioconazole single agent and the prothioconazole and metalaxyl-M compound preparation thereof have better control effect on the southern blight of the peanut, and prothioconazole EC50The content of the prothioconazole is 0.798mg/L, and the prothioconazole and metalaxyl-M are in a range of 10: 1-1: 10 to show a synergistic effect, wherein the ratio of prothioconazole: the metalaxyl-M has better activity in the mass ratio of 3:2 and EC50The toxicity coefficient is 145.887, and the toxicity coefficient is 0.547 mg/L.
TABLE 1 indoor bioassay results of prothioconazole, metalaxyl-M and their mixed mixture for peanut southern blight
Note: 1. prothioconazole: the concentration of the metalaxyl-M mixed solution refers to the concentration of prothioconazole;
2. metalaxyl-M has no obvious inhibitory activity to the bacterium causing the peanut southern blight when the maximum concentration of metalaxyl-M is designed to be 200mg/L, and EC can not be calculated50And a virulence regression equation.
Indoor example 2
Indoor toxicity determination of peanut root rot
Pathogenic bacteria to be tested: pythium mycoides (Pythium mycotylum) and Fusarium oxysporum (Fusarium oxysporum) were provided by the research and development center of the group.
Preparing a medicament: the prothioconazole and metalaxyl-M original drugs are dissolved by acetone and then diluted by 0.1 percent of Tween-80 aqueous solution. Preparing single-dose mother liquor respectively, designing 5 groups of proportioning according to the mixing purpose and the medicament activity, and preparing 5 series of mass concentrations of each single dose and each group of proportioning mixing agent according to an equal ratio method.
Under aseptic operation conditions, according to test treatment, 45ml of a pre-melted sterile culture medium is added into a sterile conical flask, 5ml of liquid medicine is sequentially sucked from low concentration to high concentration, and is respectively added into the conical flasks, fully shaken, and then poured into 3 culture dishes in equal amount to prepare a medicine-containing plate with corresponding concentration. The test was run with no agent treatment as a blank, which was repeated 3 times per treatment.
The cultured pathogenic bacteria are cut from the edge of a bacterial colony by a sterilization puncher with the diameter of 5mm under the aseptic operation condition, the bacterial cake is inoculated to the center of a drug-containing flat plate by an inoculator, the hypha surface is upward, 1 bacterial cake is placed in each culture dish, the dish cover is covered, and the culture dish is placed in an incubator (26 +/-0.5 ℃) for culture.
Data statistics and analysis:
and (5) investigating the growth condition of the hyphae of the pathogenic bacteria according to the growth condition of the bacteria in the blank control culture dish. The colony diameter was measured in millimeters (mm) with a caliper. The diameter of each colony was measured perpendicularly by the cross method once and averaged.
According to the investigation results, the hypha growth inhibition rate of each treatment concentration on the test target bacteria is calculated, and the calculation result in percentage (%) retains two decimal points.
D＝D1-D2
In the formula:
d-colony growing diameter;
D1-colony diameter;
D2-the diameter of the cake.
In the formula:
i-hypha growth inhibition rate;
D0-growing the diameter of the blank colony;
DTmedicament-treated colonies grow in diameter.
And (4) performing regression analysis according to the concentration logarithm value of each medicament and the corresponding probability value of the hypha growth inhibition rate. Finding EC of virulence regression line50Value and phaseAnd (4) a correlation coefficient r.
Sun Yupei method: evaluating the synergistic effect of the mixed medicament according to the co-toxicity coefficient (CTC), wherein the compounded co-toxicity coefficient (CTC) is more than or equal to 120 and shows the synergistic effect; CTC is less than or equal to 80 and shows antagonism; 80 < CTC < 120 showed additive effects.
Co-toxicity coefficient (CTC value) calculation of the blend:
in the formula:
ATI-actually measured toxicity index of mixed agent;
S-EC of Standard Fungicide50In milligrams per liter (mg/L);
EC of M-mixtures50In milligrams per liter (mg/L).
TTI＝TIA*PA+TIB*PB
In the formula:
TTI-mixture theory virulence index;
TIA-agent virulence index a;
PA-the percentage of agent a in the mixture in percent (%);
TIB-agent B virulence index;
PB-the percentage of the agent B in the mixture in percent (%).
In the formula:
CTC-co-toxicity coefficient;
ATI-actually measured toxicity index of mixed agent;
TTI-mixture theory virulence index.
Results and analysis:
the two agents of the prothioconazole and the metalaxyl-M have different action mechanisms, and the compound use is beneficial to overcoming or delaying the development of drug resistance of germs and improving the prevention and treatment effect.
Test results show that as shown in Table 2, 2 single agents and compound preparations thereof have better control effect on peanut root rot fungi (pythium aggregatum), and prothioconazole EC50Is 2.474mg/L, metalaxyl-M EC50It was 0.364 mg/L. The prothioconazole and the metalaxyl-M are 7: 1-1: 5, which both show a synergistic effect, wherein the ratio of prothioconazole: the metalaxyl-M has better proportioning activity of 3:2, wherein the cotoxicity coefficient is 215.456.
TABLE 2 indoor bioassay results of prothioconazole, metalaxyl-M and their mixed preparation on peanut root rot (Humicola nepalensis)
Test results show that as shown in Table 3, the prothioconazole single agent and the compound preparation of prothioconazole and metalaxyl-M have better control effect on peanut root rot (fusarium oxysporum), and prothioconazole EC50It was 1.149 mg/L. The prothioconazole and the metalaxyl-M are 7: 1-1: 5, which both show a synergistic effect, wherein the ratio of prothioconazole: the metalaxyl-M has better proportioning activity of 3:2, wherein the cotoxicity coefficient is 152.590.
TABLE 3 indoor bioassay results of prothioconazole, metalaxyl-M and their mixed preparation on peanut root rot (fusarium oxysporum) bacteria
Note: 1. prothioconazole: the concentration of the metalaxyl-M mixed solution refers to the concentration of prothioconazole;
2. metalaxyl-M has no obvious inhibition activity on peanut root rot (fusarium oxysporum) when the maximum concentration of metalaxyl-M is designed to be 200mg/L, and EC can not be calculated50And a virulence regression equation.
Preparation example 1
20% Prothioconazole-M-metalaxyl seed treatment suspending agent (10+10)
Dispersing alkyl naphthalene sulfonate formaldehyde condensate 2%, EO-PO block copolymer 1%, sodium lignosulfonate 2% and organic silicon defoamer 0.5%, magnesium aluminum silicate 0.5%, propylene glycol 4%, warning color 5% in a proper amount of water, dispersing prothioconazole 10% and mefenoxam 10% therein, and wet-pulverizing with sand mill to D using zirconia beads90(particle diameter of 90% of particles) < 5 μm, to obtain a pulverized slurry. Adding 0.1% of magnesium aluminum silicate, 0.6% of sodium p-hydroxybenzoate and 5% of polyacrylic acid (805) into the crushed slurry, uniformly mixing, complementing deionized water to 100%, and uniformly shearing at high speed to obtain the seed treatment suspending agent with the mass content of the effective component of 20 wt%.
Preparation example 2
15% Prothioconazole-metalaxyl-M seed treatment suspending agent (9+6)
Dispersing 3% of alkyl naphthalene sulfonate formaldehyde condensate, 0.8% of EO-PO block copolymer, 2% of sodium dodecyl sulfate and 0.45% of organic silicon defoamer, 0.5% of magnesium aluminum silicate, 4% of propylene glycol and 5% of warning color in a proper amount of water, dispersing 9% of prothioconazole and 6% of metalaxyl-M in the mixture, and performing wet grinding to D with a sand mill by using zirconia beads90(particle diameter of 90% of particles) < 5 μm, to obtain a pulverized slurry. Adding 0.2% of magnesium aluminum silicate, 0.5% of sodium benzoate and 5% of polyacrylic acid (805) into the crushed slurry, uniformly mixing, complementing deionized water to 100%, and uniformly shearing at high speed to obtain the seed treatment suspending agent with the mass content of the effective component of 15 wt%.
Preparation example 3
20% Prothioconazole-metalaxyl-M seed treatment suspending agent (15+5)
Dispersing 3% of alkyl naphthalene sulfonate formaldehyde condensate, 1% of EO-PO block copolymer, 2% of sodium dodecyl sulfate and 0.5% of organic silicon defoamer, 0.5% of magnesium aluminum silicate, 4% of propylene glycol and 5% of warning color in a proper amount of water, dispersing 15% of prothioconazole and 5% of mefenoxam in the mixture, and performing wet grinding by a sand mill to D by using zirconia beads90(particle diameter of 90% of particles) < 5 μm, to obtain a pulverized slurry. Adding 0.1% of magnesium aluminum silicate, 0.5% of sodium benzoate and 5% of polyacrylic acid (805) into the crushed slurry, uniformly mixing, and supplementing deionized water until the mixture is full100 percent, and uniformly shearing at high speed to prepare the seed treatment suspending agent with the mass content of the effective component of 20 weight percent.
Preparation example 4
26% Prothioconazole-M-metalaxyl seed treatment suspending agent (14+12)
Dispersing 1.5% of sodium lignin sulfonate, 1.5% of polycarboxylate, 2% of sodium dodecyl sulfate and 0.5% of organic silicon defoamer in a proper amount of water, 0.5% of magnesium aluminum silicate, 4% of propylene glycol and 5% of warning color, dispersing 14% of prothioconazole and 12% of metalaxyl-M in the mixture, and performing wet grinding to D by using a sand mill by using zirconia beads90(particle diameter of 90% of particles) < 5 μm, to obtain a pulverized slurry. 0.1 percent of xanthan gum, 0.5 percent of sodium benzoate and 5 percent of polyacrylic acid (805) are added into the crushed slurry and evenly mixed, deionized water is complemented to 100 percent, and the seed treatment suspending agent with the mass content of the effective component of 26 percent by weight is obtained after even high-speed shearing.
Preparation example 5
30% prothioconazole-metalaxyl-M seed treatment suspending agent (20+10)
Dispersing sodium lignin sulfonate 2.5%, polycarboxylate 1%, sodium dodecyl sulfate 1.5% and organic silicon defoamer 0.5%, magnesium aluminum silicate 0.5%, propylene glycol 5%, warning color 5%, in which prothioconazole 20% and mefenoxam 10% are dispersed, using zirconia beads, and wet-pulverizing with sand mill to D90(particle diameter of 90% of particles) < 5 μm, to obtain a pulverized slurry. 0.1 percent of xanthan gum, 0.6 percent of sodium p-hydroxybenzoate and 5 percent of polyacrylic acid (805) are added into the crushed slurry and uniformly mixed, deionized water is complemented to 100 percent, and the seed treatment suspending agent with the mass content of the effective component of 30 percent by weight is uniformly sheared at high speed.
Preparation example 6
28% Flucloxacarbdiamide prothioconazole mefenoxam seed treatment suspending agent (16+12)
Dispersing sodium lignin sulfonate 2.4%, EO-PO block copolymer 1.5%, sodium dodecyl sulfate 1.5% and organosilicon defoaming agent 0.5%, magnesium aluminum silicate 0.5%, propylene glycol 4%, warning color 5%, dispersing prothioconazole 16% and metalaxyl-M12% in right amount of water, using zirconium oxideWet pulverizing the beads to D with a sand mill90(particle diameter of 90% of particles) < 5 μm, to obtain a pulverized slurry. 0.1 percent of xanthan gum, 0.5 percent of sodium benzoate and 5 percent of polyacrylic acid (805) are added into the crushed slurry and evenly mixed, deionized water is complemented to 100 percent, and the seed treatment suspending agent with the mass content of the effective component of 28 percent is obtained after even high-speed shearing.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
In China, due to changes of farming systems in recent years, due to factors such as perennial continuous cropping of part of peanuts in peanut producing areas, single peanut varieties and the like, southern blight and root rot of the peanuts grow more and more year by year, and the occurrence area of southern blight and root rot of the peanuts is enlarged day by day, particularly in southern blight and root rot of main peanut producing areas such as Guangxi, Hubei, Shandong, Tianjin, Sichuan and Liaoning.
Field example 1
Peanut southern blight field efficacy test
Test subjects: the Sclerotium rolfsii Sacc is the pathogenic bacterium of southern peanut Sclerotium rolfsii, which belongs to the infection of fungi of deuteromycotina.
And (3) test crops: peanut (fu hua No. 8);
the peanut planting specification is as follows: ridge spacing of 80cm, ridge surface width of 55cm, ridge up spacing of 30cm, hole spacing of 16.5cm, peanut density of 15 ten thousand holes/hm22 seeds per hole are cultivated in a mulching film.
Test site: chongxi province of Shanghai province, China Tomby county, Tomen town peanut.
The test field has good irrigation facilities, the cultivation conditions (soil type, water and fertilizer management, planting density and growth period) of test districts are uniform and consistent, and the test field conforms to the agricultural practice (GAP) of local science.
Cell area and repetition: cell area 15m2Repeat 4 times.
Investigation time and number of times: 2 times of investigation are carried out in total, wherein the first investigation is carried out for investigating the emergence rate 10 days after peanut sowing; the 2 nd survey is a disease survey conducted 7 days before peanut harvest.
The using method comprises the following steps: weighing the pesticide and the peanut seeds according to the using amount of the pesticide, pouring the seeds into a seed mixing container one day before sowing, pouring the pesticide onto the peanut seeds, turning over while pouring, fully stirring uniformly, pushing aside and airing after the surface color of all the peanuts is consistent, and sowing.
The investigation method comprises the following steps: observing whether the pesticide injury occurs and symptoms of the pesticide injury occur from the time after the peanut seedlings emerge to the time before the peanut is harvested.
Sampling at 5 points of diagonal lines of each cell, investigating 20 piers at each point and 100 piers at each cell, surveying for the second time, pulling out the root of the peanut from one pier to another, recording the total plant number and the plant number of various diseases of southern blight, and calculating the prevention and treatment effect.
The grading method comprises the following steps:
level 0: no disease;
level 1: the peanut root is diseased, and 10 percent of diseases occur;
and 3, level: the peanut stem base generates obvious pathological changes, white hypha appears, and 30 percent of diseases occur;
and 5, stage: the peanut stem base part becomes black, white hyphae are obvious, and 50 percent of disease occurs;
and 7, stage: white hypha is fully distributed on the root of the whole peanut plant, the whole pier begins to wither, and 80 percent of diseases occur;
and 9, stage: the white hyphae of the whole peanut plant are completely covered, and the root is completely blackened and dead.
The drug effect calculation method comprises the following steps:
rate of emergence (%) — number of emergence/number of sowing × 100
Disease index (%) - [ sigma (number of diseased plants at each stage × relative stage)/(total number of investigated plants × highest stage) × 100
The preventing and treating effect (%) is (disease index of control area-disease index of treatment area)/disease index of control area x 100
After the peanuts are sowed and emerge and before the peanuts are harvested, the growth condition and the growth phase of the peanuts in the field are observed irregularly, and no visible obvious phytotoxicity symptom is found.
The control effect is shown in table 4, the mixed medicament shows obvious control effect on the peanut southern blight, and compared with blank control medicament treatment, the control medicament has the advantages of reduced morbidity index and better control effect.
TABLE 4 prevention and treatment effects of the test agents on southern blight of peanuts
Note: the control (%) in the table above is the average of each repetition; capital letters indicate that the level difference is significant at 1%.
Field example 2
Peanut root rot field efficacy test
Test subjects: root rot of peanut, and pathogenic bacteria causing the disease are mainly Fusarium solani [ Fusarium solani (Mart) Sacc ] and Fusarium oxysporum (Fusarium oxysporum Schlecht).
And (3) test crops: peanut (fu hua No. 8);
the peanut planting specification is as follows: ridge spacing of 80cm, ridge surface width of 55cm, ridge up spacing of 30cm, hole spacing of 16.5cm, peanut density of 15 ten thousand holes/hm22 seeds per hole are cultivated in a mulching film.
Test site: chongxi province of Shanghai province, China Tomby county, Tomen town peanut. The test field has good irrigation facilities, the cultivation conditions (soil type, water and fertilizer management, planting density and growth period) of test districts are uniform and consistent, and the test field conforms to the agricultural practice (GAP) of local science.
Cell area and repetition: cell area 15m2Repeat 4 times.
Investigation time and number of times: 2 times of investigation are carried out in total, wherein the first investigation is carried out for investigating the emergence rate 10 days after peanut sowing; the 2 nd investigation is the investigation of the disease condition 60 days after the peanut seedlings emerge.
The using method comprises the following steps: weighing the pesticide and the peanut seeds according to the using amount of the pesticide, pouring the seeds into a seed mixing container one day before sowing, pouring the pesticide onto the peanut seeds, turning over while pouring, fully stirring uniformly, pushing aside and airing after the surface color of all the peanuts is consistent, and sowing.
The investigation method comprises the following steps: observing whether the pesticide injury occurs and symptoms of the pesticide injury occur from the time after the peanut seedlings emerge to the time before the peanut is harvested.
Sampling at 5 points of diagonal lines of each cell, investigating 20 piers at each point, investigating 100 piers in each cell, investigating for the second time, pulling out the root of the peanut from the piers, recording the total plant number and the plant numbers of all levels of root rot, and calculating the control effect.
The grading method comprises the following steps:
level 0: no disease;
level 1: the root system changes color slightly, the color-changed root system accounts for less than 10 percent of the whole root system, and the plant does not wither;
and 3, level: the root system is obviously browned, the discolored root system accounts for 10.1 to 30 percent of the total root system, and the plant begins to wilt;
and 5, stage: the color-changing root system accounts for 30.1-50% of the total root system, and the plant is wilted obviously;
and 7, stage: the color-changing root system accounts for 50.1-80% of the total root system, and the plant wilts;
and 9, stage: the whole plant died.
The drug effect calculation method comprises the following steps:
rate of emergence (%) — number of emergence/number of sowing × 100
Disease index (%) - [ sigma (number of diseased plants at each stage × relative stage)/(total number of investigated plants × highest stage) × 100
The preventing and treating effect (%) is (disease index of control area-disease index of treatment area)/disease index of control area x 100
After the peanuts are sowed and emerge and before the peanuts are harvested, the growth condition and the growth phase of the peanuts in the field are observed irregularly, and no visible obvious phytotoxicity symptom is found.
The control effect is shown in table 5, the binary mixed medicament shows obvious control effect on flower root rot, and compared with blank control medicament treatment, the control effect has the advantages of reduced morbidity index and better control effect.
TABLE 5 prevention and treatment effects of the test agents on peanut root rot
Note: the control (%) in the table above is the average of each repetition; capital letters indicate that the level difference is significant at 1%.
Through indoor toxicity measurement and field tests, the prothioconazole and the metalaxyl-M are compounded to show good prevention and control effects on the southern blight and the root rot of the peanut. The pesticide composition or the preparation thereof obtained by compounding has obvious control effect, is superior to a single agent in the aspects of delaying the generation of drug resistance and prolonging the drug-holding property, and simultaneously comprises the seed treatment suspending agent of the active ingredients of prothioconazole and metalaxyl-M, so that the dosage form ensures that the seed coating is fast in film forming and does not fall off, and the pesticide composition or the preparation thereof is safe to crop seeds and has good tolerance. And no phytotoxicity of the compound pesticide on crops is found in tests, which shows that the production cost and the use cost can be reduced and the pesticide composition or the preparation is safe to the crops under the condition of improved sterilization synergy.
Although the invention has been described in detail with respect to the general description and the specific embodiments, it will be apparent to those skilled in the art that modifications or improvements may be made thereto without departing from the spirit of the invention.
Claims (10)
1. The application of the bactericidal composition containing prothioconazole and metalaxyl-M in controlling peanut diseases is characterized in that the bactericidal composition at least comprises an active ingredient A prothioconazole and an active ingredient B metalaxyl-M, and the mass ratio of the active ingredient A prothioconazole to the active ingredient B metalaxyl-M is 50: 1-1: 50.
2. The bactericidal composition according to claim 1, wherein the mass ratio of the prothioconazole as an active ingredient A to the metalaxyl-M as an active ingredient B is 10: 1-1: 10.
3. The bactericidal composition according to claim 1, wherein the mass ratio of the prothioconazole as an active ingredient A to the metalaxyl-M as an active ingredient B is 7: 1-1: 5;
preferably, the mass ratio of the prothioconazole as the active ingredient A to the metalaxyl-M as the active ingredient B is 3: 1-3: 4.
4. The bactericidal composition of claim 1, wherein the sum of the contents of the active ingredient a and the active ingredient B in the bactericidal composition is 0.1% to 80%, preferably 2% to 50%, and more preferably 2% to 30%, based on 100% by weight of the total bactericidal composition.
5. The germicidal composition of claim 1, further comprising an adjuvant selected from one or more of a wetting agent, a dispersing agent, an emulsifier, a thickener, a disintegrant, an anti-freeze agent, an anti-foaming agent, a solvent, a preservative, a stabilizer, a warning color, a film-forming agent, a synergist, and a carrier.
6. The bactericidal composition of claim 5, wherein the bactericidal composition is in any agriculturally acceptable dosage form;
preferably, the dosage form of the bactericidal composition is any one of aqueous solution, wettable powder, microemulsion, aqueous emulsion, suspending agent, missible oil, water dispersible granules, suspended seed coating agent, seed treatment suspending agent, microcapsule suspending agent and dry seed mixing agent; preferably, the formulation of the bactericidal composition is a seed treatment suspending agent.
7. The use of the bactericidal composition of claim 1 for controlling peanut diseases, wherein the peanut diseases are bacterial diseases or fungal diseases.
8. The use of the bactericidal composition according to claim 7 for controlling peanut diseases, wherein the peanut diseases are any one or more of peanut black spot disease, peanut brown spot disease, peanut net spot disease, peanut anthracnose, peanut coke spot disease, peanut purple stripe disease, peanut rust disease, peanut bacterial wilt, peanut stem rot, peanut southern blight, peanut root rot, peanut yellow mosaic virus, peanut stripe virus disease, peanut dwarf virus disease and peanut crown rot.
9. The use of the bactericidal composition according to claim 7 for controlling peanut diseases, wherein the peanut diseases are peanut southern blight and peanut root rot.
10. The use of the bactericidal composition according to claim 7 for controlling peanut diseases, wherein the bactericidal composition is applied to diseases to be controlled or a growth medium thereof in an effective dose.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111558047.6A CN114176090A (en) | 2021-12-20 | 2021-12-20 | Application of bactericidal composition containing prothioconazole and metalaxyl-M in preventing and treating peanut diseases |
| CN202211443843.XA CN116210703A (en) | 2021-12-20 | 2022-11-18 | Application of sterilization composition containing prothioconazole and metalaxyl-M in preventing and treating peanut diseases |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111558047.6A CN114176090A (en) | 2021-12-20 | 2021-12-20 | Application of bactericidal composition containing prothioconazole and metalaxyl-M in preventing and treating peanut diseases |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114176090A true CN114176090A (en) | 2022-03-15 |
Family
ID=80605556
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111558047.6A Pending CN114176090A (en) | 2021-12-20 | 2021-12-20 | Application of bactericidal composition containing prothioconazole and metalaxyl-M in preventing and treating peanut diseases |
| CN202211443843.XA Pending CN116210703A (en) | 2021-12-20 | 2022-11-18 | Application of sterilization composition containing prothioconazole and metalaxyl-M in preventing and treating peanut diseases |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211443843.XA Pending CN116210703A (en) | 2021-12-20 | 2022-11-18 | Application of sterilization composition containing prothioconazole and metalaxyl-M in preventing and treating peanut diseases |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN114176090A (en) |
-
2021
- 2021-12-20 CN CN202111558047.6A patent/CN114176090A/en active Pending
-
2022
- 2022-11-18 CN CN202211443843.XA patent/CN116210703A/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN116210703A (en) | 2023-06-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104365611B (en) | A kind of bactericidal composition and application containing amisulbrom and mandipropamid | |
| CN114617127B (en) | Pesticide sterilization composition and application thereof | |
| CN111183995B (en) | Application of pesticide composition containing fludioxonil and thifluzamide to prevention and treatment of peanut diseases or insect pests | |
| CN105475335A (en) | Suspension seed coating containing thifluzamide, mefenoxam and thiamethoxam | |
| CN113966743B (en) | Sterilization mixture containing succinate dehydrogenase inhibitor and application thereof | |
| CN112514903B (en) | Pesticide composition containing fenpyroximate and application thereof | |
| CN114617129A (en) | Bactericidal composition and application thereof | |
| CN114176090A (en) | Application of bactericidal composition containing prothioconazole and metalaxyl-M in preventing and treating peanut diseases | |
| CN108184861A (en) | A kind of bactericidal composition and its application containing pyraziflumid | |
| CN114097803B (en) | Pesticide composition containing prothioconazole and application thereof | |
| CN115316394B (en) | Sterilization composition containing fluazinam | |
| CN114747582B (en) | Pesticide composition containing Isoflucypram and copper quinolinate and application thereof | |
| CN114766492B (en) | Pesticide composition containing bixafen and application thereof | |
| CN114451416B (en) | Pesticide composition containing benzovindiflupyr and application thereof | |
| CN114158565B (en) | Sterilization composition containing amifepin and chlorphenamine amide and application thereof | |
| CN117099795A (en) | Application of pesticide composition containing prothioconazole and fludioxonil in preventing and controlling wheat stem-based rot and improving wheat growth activity | |
| CN102057907B (en) | Sterilization composite containing dithianon and chlorothalonil and application thereof | |
| CN117770267A (en) | Mite-killing composition and application thereof | |
| CN115885998A (en) | Application of bactericidal composition containing mandipropamid and fluopicolide in preventing and treating phytophthora diseases | |
| CN117063935A (en) | Sterilization composition and application thereof | |
| CN115104621A (en) | Bactericidal composition containing fosetyl-aluminum and application thereof | |
| CN117617256A (en) | Nematicidal composition and application thereof in preventing and controlling nematode infection and development on crops | |
| CN114766483A (en) | Sterilization composition containing bronopol and application thereof | |
| CN116369326A (en) | Sterilization composition containing tetramycin and tricyclazole and application thereof | |
| CN116998498A (en) | Pesticide composition containing cyantraniliprole and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20220315 |
|
| WD01 | Invention patent application deemed withdrawn after publication |