CN117598964A - Tea and caviar composition with repairing, antioxidant, anti-wrinkle and tightening effects and application thereof - Google Patents
Tea and caviar composition with repairing, antioxidant, anti-wrinkle and tightening effects and application thereof Download PDFInfo
- Publication number
- CN117598964A CN117598964A CN202311528473.4A CN202311528473A CN117598964A CN 117598964 A CN117598964 A CN 117598964A CN 202311528473 A CN202311528473 A CN 202311528473A CN 117598964 A CN117598964 A CN 117598964A
- Authority
- CN
- China
- Prior art keywords
- caviar
- culture medium
- tea
- composition
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 90
- ZPUCINDJVBIVPJ-LJISPDSOSA-N cocaine Chemical compound O([C@H]1C[C@@H]2CC[C@@H](N2C)[C@H]1C(=O)OC)C(=O)C1=CC=CC=C1 ZPUCINDJVBIVPJ-LJISPDSOSA-N 0.000 title claims abstract description 76
- 230000000694 effects Effects 0.000 title claims abstract description 43
- 230000001153 anti-wrinkle effect Effects 0.000 title claims abstract description 32
- 230000003078 antioxidant effect Effects 0.000 title claims abstract description 22
- 239000003963 antioxidant agent Substances 0.000 title claims abstract description 19
- 238000000855 fermentation Methods 0.000 claims abstract description 102
- 230000004151 fermentation Effects 0.000 claims abstract description 102
- 239000000284 extract Substances 0.000 claims abstract description 54
- 241000972773 Aulopiformes Species 0.000 claims abstract description 45
- 235000019515 salmon Nutrition 0.000 claims abstract description 45
- 241000881711 Acipenser sturio Species 0.000 claims abstract description 44
- 229940111782 egg extract Drugs 0.000 claims abstract description 38
- 239000002537 cosmetic Substances 0.000 claims abstract description 9
- 244000269722 Thea sinensis Species 0.000 claims description 86
- 239000001963 growth medium Substances 0.000 claims description 67
- 244000235858 Acetobacter xylinum Species 0.000 claims description 62
- 235000002837 Acetobacter xylinum Nutrition 0.000 claims description 62
- 235000013616 tea Nutrition 0.000 claims description 52
- 235000006468 Thea sinensis Nutrition 0.000 claims description 35
- 235000020279 black tea Nutrition 0.000 claims description 35
- 241000235347 Schizosaccharomyces pombe Species 0.000 claims description 33
- 239000007788 liquid Substances 0.000 claims description 30
- 238000009630 liquid culture Methods 0.000 claims description 29
- 239000007787 solid Substances 0.000 claims description 24
- 150000001875 compounds Chemical class 0.000 claims description 23
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 21
- 239000000843 powder Substances 0.000 claims description 21
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 20
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 20
- 241000235342 Saccharomycetes Species 0.000 claims description 20
- 238000002360 preparation method Methods 0.000 claims description 20
- 239000008103 glucose Substances 0.000 claims description 16
- 239000002609 medium Substances 0.000 claims description 16
- 230000001954 sterilising effect Effects 0.000 claims description 14
- 230000004913 activation Effects 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 12
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 10
- 230000001580 bacterial effect Effects 0.000 claims description 10
- 229910052799 carbon Inorganic materials 0.000 claims description 10
- 229910052757 nitrogen Inorganic materials 0.000 claims description 10
- 239000000725 suspension Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 239000001888 Peptone Substances 0.000 claims description 9
- 108010080698 Peptones Proteins 0.000 claims description 9
- 244000061456 Solanum tuberosum Species 0.000 claims description 9
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 9
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 claims description 9
- 229960005091 chloramphenicol Drugs 0.000 claims description 9
- 235000019319 peptone Nutrition 0.000 claims description 9
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- 244000068988 Glycine max Species 0.000 claims description 8
- 235000010469 Glycine max Nutrition 0.000 claims description 8
- 229930006000 Sucrose Natural products 0.000 claims description 8
- 239000005720 sucrose Substances 0.000 claims description 8
- 229920001817 Agar Polymers 0.000 claims description 7
- 239000008272 agar Substances 0.000 claims description 7
- 239000002054 inoculum Substances 0.000 claims description 7
- 238000000265 homogenisation Methods 0.000 claims description 6
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 claims description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 5
- 229940041514 candida albicans extract Drugs 0.000 claims description 5
- 239000008121 dextrose Substances 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 5
- 239000012138 yeast extract Substances 0.000 claims description 5
- 229930091371 Fructose Natural products 0.000 claims description 4
- 239000005715 Fructose Substances 0.000 claims description 4
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 235000015067 sauces Nutrition 0.000 claims description 4
- 239000012137 tryptone Substances 0.000 claims description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 2
- 230000008439 repair process Effects 0.000 abstract description 14
- 108010022452 Collagen Type I Proteins 0.000 abstract description 11
- 230000005764 inhibitory process Effects 0.000 abstract description 10
- 108020004999 messenger RNA Proteins 0.000 abstract description 10
- 230000029663 wound healing Effects 0.000 abstract description 8
- 102000012422 Collagen Type I Human genes 0.000 abstract description 7
- 108010016113 Matrix Metalloproteinase 1 Proteins 0.000 abstract description 7
- 230000005012 migration Effects 0.000 abstract description 7
- 238000013508 migration Methods 0.000 abstract description 7
- 102000000380 Matrix Metalloproteinase 1 Human genes 0.000 abstract description 6
- 230000003712 anti-aging effect Effects 0.000 abstract description 4
- -1 DPPH free radical Chemical class 0.000 abstract description 3
- 230000004888 barrier function Effects 0.000 abstract description 2
- 241001122767 Theaceae Species 0.000 abstract 5
- 230000000052 comparative effect Effects 0.000 description 62
- 239000000243 solution Substances 0.000 description 45
- 210000004027 cell Anatomy 0.000 description 27
- 108010035532 Collagen Proteins 0.000 description 23
- 102000008186 Collagen Human genes 0.000 description 22
- 229920001436 collagen Polymers 0.000 description 22
- 210000003491 skin Anatomy 0.000 description 15
- 239000000835 fiber Substances 0.000 description 13
- 238000012360 testing method Methods 0.000 description 11
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 10
- 238000012258 culturing Methods 0.000 description 10
- 239000000523 sample Substances 0.000 description 10
- 210000002966 serum Anatomy 0.000 description 10
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 9
- 239000012467 final product Substances 0.000 description 9
- 210000002510 keratinocyte Anatomy 0.000 description 9
- 210000004177 elastic tissue Anatomy 0.000 description 8
- 238000011081 inoculation Methods 0.000 description 8
- 230000032683 aging Effects 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 7
- 102000002274 Matrix Metalloproteinases Human genes 0.000 description 6
- 108010000684 Matrix Metalloproteinases Proteins 0.000 description 6
- 239000011248 coating agent Substances 0.000 description 6
- 238000000576 coating method Methods 0.000 description 6
- 230000002195 synergetic effect Effects 0.000 description 6
- 206010040954 Skin wrinkling Diseases 0.000 description 5
- 239000013543 active substance Substances 0.000 description 5
- 239000000706 filtrate Substances 0.000 description 5
- 229920001184 polypeptide Polymers 0.000 description 5
- 108090000765 processed proteins & peptides Proteins 0.000 description 5
- 102000004196 processed proteins & peptides Human genes 0.000 description 5
- 230000037303 wrinkles Effects 0.000 description 5
- 108010014258 Elastin Proteins 0.000 description 4
- 102000016942 Elastin Human genes 0.000 description 4
- 238000013329 compounding Methods 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 229920002549 elastin Polymers 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 150000003254 radicals Chemical class 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 210000004207 dermis Anatomy 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000013642 negative control Substances 0.000 description 3
- 239000012488 sample solution Substances 0.000 description 3
- 125000000185 sucrose group Chemical group 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 2
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 238000011529 RT qPCR Methods 0.000 description 2
- 230000003064 anti-oxidating effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 210000002744 extracellular matrix Anatomy 0.000 description 2
- 235000019688 fish Nutrition 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 239000010410 layer Substances 0.000 description 2
- 210000003936 merozoite Anatomy 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 239000007218 ym medium Substances 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000012292 cell migration Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 239000006481 glucose medium Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 125000001312 palmitoyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
- 230000004224 protection Effects 0.000 description 1
- 230000009979 protective mechanism Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 230000009759 skin aging Effects 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 229960000344 thiamine hydrochloride Drugs 0.000 description 1
- 235000019190 thiamine hydrochloride Nutrition 0.000 description 1
- 239000011747 thiamine hydrochloride Substances 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
- 230000037373 wrinkle formation Effects 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/90—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation
Abstract
The application relates to the technical field of cosmetics, and particularly discloses a tea and caviar composition with repairing, antioxidant and anti-wrinkle tightening effects and application thereof. The tea and caviar composition of the present application includes a fermentation complex, salmon egg extract, and sturgeon caviar extract. The tea and caviar composition has good DPPH free radical inhibition effect, can promote migration of cells in a scratch area, and has the potential of wound healing and barrier repair; meanwhile, the tea and caviar composition can promote the expression of collagen COL-I mRNA and inhibit the expression of MMP-1 mRNA. The tea and caviar composition has good antioxidant, anti-wrinkle, repairing and anti-aging effects.
Description
Technical Field
The application relates to the technical field of cosmetics, in particular to a tea and caviar composition with repairing, antioxidant and anti-wrinkle tightening effects and application thereof.
Background
Along with the continuous acceleration of the life rhythm and the increasing severity of environmental problems, the problems of regulation of in vivo hormones and invasion of external harmful substances of skin tissues easily cause the weakening of the synthesis capacity of collagen and elastin of human skin, and the aging problem of skin also occurs.
The existing anti-wrinkle cosmetics generally take active substances of polypeptides as active ingredients for anti-wrinkle of cosmetics, such as acetyl polypeptide active substances, palmitoyl polypeptide active substances and the like; because of the barrier effect of the epidermis and the horny layer of the skin, the molecular weight of the active substances of polypeptides is large, and the active substances of polypeptides are mostly difficult to be absorbed through skin, so that the anti-aging effect of cosmetics is poor, and therefore, there is room for improvement.
Disclosure of Invention
The present application aims to overcome the above-mentioned shortcomings of the prior art and provide a tea and caviar composition with repairing, antioxidant and anti-wrinkle tightening effects and its application.
In order to achieve the above purpose, the technical scheme adopted by the application is as follows:
the present application provides a tea and caviar composition having repair, antioxidant and anti-wrinkle tightening effects, the composition comprising a fermentation complex, salmon egg extract and sturgeon caviar extract;
the fermentation compound is obtained by fermenting saccharomycetes, acetobacter xylinum and black tea.
The applicant finds that the tea and caviar composition obtained by fermenting saccharomycetes, acetobacter xylinum and black tea to obtain a fermentation compound and then compounding salmon egg extract and sturgeon caviar extract has good DPPH free radical inhibition effect through multiple experiments. At the cell level, the tea and caviar composition can promote migration of cells in a scratch area, and has the potential of wound healing; meanwhile, the tea and caviar composition can well promote the expression of collagen COL-I mRNA, inhibit the expression of MMP-1mRNA and promote the expression of collagen COL-I mRNA, so that collagen is more easily synthesized by cells, the expression of MMP-1mRNA is inhibited, the normal structure of collagen fibers and elastic fibers is less easily damaged, and the collagen fibers and the elastic fibers are more easily maintained. The composition can remove free radicals to a certain extent on macroscopic manifestation, and can resist wrinkle and aging better.
When the fermentation complex is fermented with only one fungus (acetobacter xylinum or saccharomycetes), the obtained composition has a great influence on MMP-1mRNA expression, and when the fermentation complex is fermented with only one fungus, the obtained composition has a smaller inhibition effect on MMP-1mRNA expression than the tea and caviar composition. Moreover, when the fermentation compound is fermented by only one fungus (acetobacter xylinum or saccharomycetes), the antioxidant, repairing and anti-wrinkle effects of the obtained composition are inferior to those of the tea and caviar composition.
As a preferred embodiment of the tea and caviar composition described herein, the mass ratio of the fermentation complex, salmon egg extract and sturgeon caviar extract is (2-4): (0.8-1.2), namely (0.8-1.2); preferably, the mass ratio of the fermentation complex, salmon egg extract and sturgeon roe sauce extract is 3:1:1.
The tea and caviar composition can promote migration of HaCaT human keratinocytes, and has strong repairing potential. In addition, the anti-wrinkle agent can promote the expression of COL-I genes in HaCaT human keratinocytes, and has better anti-wrinkle capability.
Wherein, there is synergism between the fermented complex, salmon egg extract and sturgeon caviar extract of this application, lack one of them component, or replace the component in it with the component with similar function, the antioxidation, repair, crease-resistant effect of the composition obtained is all inferior to tea and caviar composition of this application.
As a preferred embodiment of the tea and caviar composition described herein, the yeast is Schizosaccharomyces pombe.
In some embodiments, the schizosaccharomyces pombe is a schizosaccharomyces pombe BNCC186058 and the acetobacter xylinum is a BNCC339798 acetobacter xylinum.
As a preferred embodiment of the tea and caviar composition described herein, the process for preparing the fermentation complex comprises the steps of:
inoculating the saccharomycete seed liquid and the acetobacter xylinum seed liquid into a culture medium containing black tea powder for fermentation culture to obtain the fermentation compound.
As a preferred embodiment of the tea and caviar composition, the total inoculation amount of the saccharomycete seed liquid and the acetobacter xylinum seed liquid is 1-3%, and the inoculation mass ratio of the saccharomycete seed liquid to the acetobacter xylinum seed liquid is (2-5): 1.
as a preferred embodiment of the tea and caviar composition described herein, the black tea powder-containing medium comprises the following components in mass percent:
0.5-2% of black tea powder, 2-5% of carbon source, 0.5-2% of nitrogen source and the balance of water;
the carbon source comprises at least one of sucrose, glucose and fructose;
the nitrogen source comprises at least one of soybean peptone, tryptone and yeast extract.
The yeast seed liquid and the acetobacter xylinum seed liquid with the inoculation mass ratio are adopted, and the carbon source and the nitrogen source of the components are adopted, so that the antioxidant, repairing and crease-resistant effects of the final product can be improved.
As a preferred embodiment of the tea and caviar composition described herein, the conditions of the fermentation culture include: the temperature of the fermentation culture is 28-35 ℃, the time of the fermentation culture is 56-84 hours, and the ventilation amount of the sterile air is 0.2-0.5 vvm.
As a preferred embodiment of the tea and caviar composition described herein, the method of preparing a fermentation complex further comprises a post-treatment step comprising a high pressure homogenization treatment, a filtration treatment, and a sterilization treatment;
the pressure of the high-pressure homogenization is 8-20 MPa, and the time of the high-pressure homogenization is 20-60 min;
the temperature of the sterilization treatment is 85-105 ℃, and the time of the sterilization treatment is more than 15 min.
As a preferred embodiment of the tea and caviar composition described herein, the method for preparing the yeast seed solution is:
1) Inoculating the bacterial suspension of the saccharomycetes into a solid culture medium A for activation culture, wherein the temperature of the activation culture is 28-33 ℃, and the time of the activation culture is 24-48 hours, so as to obtain activated saccharomycetes;
2) Inoculating the activated saccharomycetes into a liquid culture medium A for expansion culture at 28-33 ℃ for 24-48 hours to obtain saccharomycetes seed liquid;
the solid culture medium A comprises at least one of YM solid culture medium, wort solid culture medium and potato glucose solid culture medium;
the liquid culture medium A comprises at least one of YM liquid culture medium, malt juice liquid culture medium and potato dextrose liquid culture medium.
As a preferred embodiment of the tea and caviar composition described herein, the preparation method of the acetobacter xylinum seed liquid comprises the following steps:
1) Inoculating the bacterial suspension of acetobacter xylinum into a solid culture medium B for activation culture, wherein the temperature of the activation culture is 28-33 ℃, and the time of the activation culture is 3-5 d, so as to obtain activated acetobacter xylinum;
2) Inoculating the activated acetobacter xylinum into a liquid culture medium B for expansion culture, wherein the expansion culture temperature is 28-33 ℃, and the expansion culture time is 3-5 d, so as to obtain acetobacter xylinum seed liquid;
the solid culture medium B comprises at least one of a yeast glucose chloramphenicol agar culture medium, a wort solid culture medium and a YM solid culture medium;
the liquid culture medium B comprises at least one of yeast glucose chloramphenicol liquid culture medium, malt juice liquid culture medium and YM liquid culture medium.
The application also provides application of the tea and caviar composition with repairing, antioxidant and anti-wrinkle effects in preparing cosmetics.
Preferably, the tea and caviar composition with repairing, antioxidant and anti-wrinkle effects is added into the cosmetic in an amount of 0.1-20% by mass.
Compared with the prior art, the application has the following beneficial effects:
the application provides a tea and caviar composition with repairing, antioxidant and anti-wrinkle tightening effects and application thereof. The fermentation compound, salmon egg extract and sturgeon caviar are compounded according to a certain proportion, and the obtained tea and caviar composition has a good DPPH free radical inhibition effect. At the cell level, the tea and caviar composition can promote migration of cells in a scratch area, and has the potential of wound healing; meanwhile, the tea and caviar composition can well promote the expression of collagen COL-I mRNA and inhibit the expression of MMP-1 mRNA; the expression of the COL-I mRNA of the collagen is promoted, which indicates that the cell can synthesize the collagen more easily; MMP-1mRNA expression was inhibited, indicating that the normal structure of collagen fibers and elastic fibers was less likely to be disrupted, and collagen fibers and elastic fibers were more likely to remain undisturbed. The tea and caviar composition has good antioxidant, anti-wrinkle, repairing and anti-aging effects on macroscopic appearance.
Detailed Description
For a better description of the objects, technical solutions and advantages of the present application, the present application will be further described with reference to specific examples.
In the following examples and comparative examples, the experimental methods used were conventional methods unless otherwise specified, and the materials, reagents and the like used, unless otherwise specified, were all commercially available.
The sturgeon roe sauce extract, salmon roe extract, roe extract and hydrolyzed roe used in the present application are raw materials in the used cosmetic raw materials catalog, and are commercially available.
The schizosaccharomyces pombe used in the application is BNCC186058 schizosaccharomyces pombe, the acetobacter xylinus used in the application is BNCC339798 acetobacter xylinus, and the strains are purchased from Beijing North Nakau biological technology research institute.
The YM medium, the yeast glucose chloramphenicol medium, the wort medium and the potato glucose medium mentioned in the application are commonly used in the prior art and can be prepared by commercial methods or the following methods:
YM medium (for example, 1L preparation): 3.0g of yeast extract, 3.0g of malt extract, 10.0g of glucose, 5.0g of peptone and 20.0g of agar (without liquid culture medium), wherein distilled water is used for sterilizing at 121 ℃ for 15min to 1.0L, and cooling for standby.
Yeast glucose chloramphenicol medium (1L for preparation): 5.0g of yeast extract, 20.0g of glucose, 15.0g of agar (liquid culture medium is not contained), 0.1g of chloramphenicol, 1.0L of distilled water with constant volume, sterilizing at 121 ℃ for 15min, and cooling for later use.
Wort medium (for example 1L preparation): 130.1g of wort culture medium powder, 10g of agar powder (liquid culture medium is not contained), distilled water is taken to be 1.0L, and the mixture is heated and boiled until the mixture is completely dissolved at 121 ℃ for 15min, sterilized and cooled for standby.
Potato dextrose medium (1L for preparation): 15g of potato soaked powder, 20g of glucose, 3g of monopotassium phosphate, 1.5g of anhydrous magnesium sulfate, 8mg of thiamine hydrochloride and 15.0g of agar (the liquid culture medium does not contain), distilled water is fixed to 1L, and the materials are uniformly mixed, sterilized at 121 ℃ for 15min and cooled for standby.
Example 1
The embodiment provides a tea and caviar composition with repairing, antioxidant and anti-wrinkle tightening effects, comprising 60 parts by weight of a fermentation compound, 20 parts by weight of salmon egg extract and 20 parts by weight of sturgeon caviar extract; the mass ratio of the fermentation compound to the salmon roe extract to the sturgeon roe sauce extract is 3:1:1.
The fermentation compound is obtained by fermenting saccharomycetes, acetobacter xylinum and black tea.
The preparation method of the fermentation complex comprises the following steps:
(1) Preparing schizosaccharomyces pombe seed liquid and acetobacter xylinum seed liquid:
coating the bacterial suspension of schizosaccharomyces pombe in YM solid culture medium, and culturing at 30 ℃ for 36 hours to obtain activated schizosaccharomyces pombe; picking the colony of activated schizosaccharomyces pombe into YM liquid culture medium, and culturing at 30deg.C for 36h to obtain schizosaccharomyces pombe seed solution.
Coating the bacterial suspension of acetobacter xylinum in a yeast glucose chloramphenicol agar medium, and culturing for 4d at 30 ℃ to obtain activated acetobacter xylinum; picking the colony of the activated acetobacter xylinum into a yeast glucose chloramphenicol liquid culture medium, and culturing for 4d at 30 ℃ to obtain acetobacter xylinum seed liquid.
(2) Preparing a culture medium containing black tea powder:
uniformly mixing 1% of black tea powder, 3% of sucrose, 1% of soybean peptone and the balance of water, and sterilizing at 121 ℃ for 15min to obtain a culture medium containing black tea powder.
(3) Fermentation treatment
Inoculating Schizosaccharomyces pombe seed solution and Acetobacter xylinum seed solution into a culture medium containing black tea powder at an inoculum size of 2% (the inoculum size of the Schizosaccharomyces pombe seed solution is 1.6%, and the inoculum size of the Acetobacter xylinum seed solution is 0.4%), and fermenting at 30deg.C under a sterile air inlet of 0.3vvm for 72 hr to obtain crude black tea fermentation broth.
(4) Post-treatment
And (3) placing the crude black tea fermentation broth in a high-pressure homogenizer, homogenizing under 10MPa for 30min, filtering the homogenized crude black tea fermentation broth, and preserving the temperature of the obtained filtrate at 90 ℃ for 20min for sterilization to obtain the fermentation compound.
Example 2
The embodiment provides a tea and caviar composition with repairing, antioxidant and anti-wrinkle tightening effects, which comprises 66.67 parts by weight of a fermentation compound, 20 parts by weight of sturgeon caviar extract and 13.33 parts by weight of salmon egg extract, wherein the mass ratio of the fermentation compound to the salmon egg extract to the sturgeon caviar extract is 4:1.2:0.8.
Wherein the fermentation complex is prepared in the same manner as in example 1.
Example 3
The embodiment provides a tea and caviar composition with repairing, antioxidant and anti-wrinkle tightening effects, which comprises 50 parts by weight of a fermentation compound, 20 parts by weight of sturgeon caviar extract and 30 parts by weight of salmon egg extract, wherein the mass ratio of the fermentation compound to the sturgeon caviar extract to the salmon egg extract is 2:0.8:1.2.
Wherein the fermentation complex is prepared in the same manner as in example 1.
Examples 4 to 7
The proportions of the fermentation complexes, salmon egg extract and sturgeon roe paste extract in examples 4 to 7 were the same as in example 1, except that the preparation method of the fermentation complexes was different from example 1, and the preparation methods of the fermentation complexes in examples 4 to 7 were different from example 1 as shown in Table 1 below:
TABLE 1
Example 8
The embodiment provides a tea and caviar composition with repairing, antioxidant and anti-wrinkle tightening effects, which comprises 50 parts by weight of a fermentation compound, 25 parts by weight of sturgeon caviar extract and 25 parts by weight of salmon egg extract, wherein the mass ratio of the fermentation compound to the sturgeon caviar extract to the salmon egg extract is 2:1:1.
The fermentation compound is obtained by fermenting saccharomycetes, acetobacter xylinum and black tea.
The preparation method of the fermentation complex comprises the following steps:
(1) Preparation of Schizosaccharomyces pombe seed solution and Acetobacter xylinum seed solution
Coating the bacterial suspension of schizosaccharomyces pombe in a wort solid culture medium, and culturing for 24 hours at 33 ℃ to obtain activated schizosaccharomyces pombe; the activated schizosaccharomyces pombe colony is picked up to a wort solid culture medium, and is cultured for 48 hours at the temperature of 33 ℃ to obtain schizosaccharomyces pombe seed liquid.
Coating the bacterial suspension of acetobacter xylinum in YM solid culture medium, and culturing for 5d at 28 ℃ to obtain activated acetobacter xylinum; picking the colony of the activated acetobacter xylinum, and culturing for 3d at 28 ℃ in YM liquid culture medium to obtain acetobacter xylinum seed liquid.
(2) Preparing a culture medium containing black tea powder:
uniformly mixing 1.5% of black tea powder, 2% of fructose, 2% of tryptone and the balance of water, and sterilizing at 121 ℃ for 15min to obtain a culture medium containing black tea powder.
(3) Fermentation treatment
Inoculating Schizosaccharomyces pombe seed solution and Acetobacter xylinum seed solution into black tea powder-containing culture medium (0.8% for Schizosaccharomyces pombe seed solution and 0.2% for Acetobacter xylinum seed solution) at 1%, fermenting at 35deg.C under aseptic air of 0.5vvm for 56 hr to obtain crude black tea fermentation broth
(4) Post-treatment
And (3) placing the crude black tea fermentation broth in a high-pressure homogenizer, homogenizing under 8MPa for 60min, filtering the homogenized crude black tea fermentation broth, and preserving the temperature of the obtained filtrate at 85 ℃ for 30min for sterilization to obtain the fermentation compound.
Example 9
The embodiment provides a tea and caviar composition with repairing, antioxidant and anti-wrinkle tightening effects, which comprises 60 parts by weight of a fermentation compound, 16 parts by weight of sturgeon caviar extract and 24 parts by weight of salmon egg extract, wherein the mass ratio of the fermentation compound to the sturgeon caviar extract to the salmon egg extract is 3:0.8:1.2.
The fermentation compound is obtained by fermenting saccharomycetes, acetobacter xylinum and black tea.
The preparation method of the fermentation complex comprises the following steps:
(1) Preparing schizosaccharomyces pombe seed liquid and acetobacter xylinum seed liquid:
coating the bacterial suspension of schizosaccharomyces pombe in a potato dextrose solid culture medium, and culturing for 48 hours at the temperature of 28 ℃ to obtain activated schizosaccharomyces pombe; the colony of activated schizosaccharomyces pombe is picked up and cultured for 24 hours at 28 ℃ in a potato dextrose liquid culture medium, so as to obtain the schizosaccharomyces pombe seed liquid.
Coating the bacterial suspension of acetobacter xylinum in a wort solid culture medium, and culturing for 3d at 33 ℃ to obtain activated acetobacter xylinum; picking the colony of the activated acetobacter xylinum into a malt juice liquid culture medium, and culturing for 5d at 33 ℃ to obtain acetobacter xylinum seed liquid.
(2) Preparing a culture medium containing black tea powder:
uniformly mixing 0.8% of black tea powder, 5% of sucrose, 0.5% of soybean peptone and the balance of water, and sterilizing at 121 ℃ for 15min to obtain a culture medium containing black tea powder.
(3) Fermentation treatment
Inoculating Schizosaccharomyces pombe seed solution and Acetobacter xylinum seed solution into black tea powder-containing culture medium (the inoculum size of Schizosaccharomyces pombe seed solution is 2% and Acetobacter xylinum seed solution is 1%) at 3%, fermenting at 28deg.C under aseptic air of 0.2vvm for 84 hr to obtain crude black tea fermentation broth
(4) Post-treatment
And (3) placing the crude black tea fermentation broth in a high-pressure homogenizer, homogenizing under 20MPa for 20min, filtering the homogenized crude black tea fermentation broth, and preserving the temperature of the obtained filtrate at 100 ℃ for 25min for sterilization to obtain the fermentation compound.
Comparative examples 1 to 6
Comparative examples 1 to 6 are similar to example 1 except that the weight parts of the fermentation complex, sturgeon roe paste extract and salmon roe extract are different, and the preparation method of the fermentation complex is the same as in example 1. The specific differences are shown in table 2.
TABLE 2
Comparative example 7
The fermented black tea filtrate prepared by the method of example 1 in the patent of application No. 202211011955.8 was used in place of the fermented complex in example 1 of the present application, and the weight parts of the fermented black tea filtrate, salmon egg extract and sturgeon caviar extract were the same as in example 1, to prepare a tea and caviar composition.
Comparative example 8
The tea and caviar composition was prepared by replacing 20 parts by weight of salmon egg extract in example 1 of the present application with 20 parts by weight of fish egg extract.
Comparative example 9
The tea and caviar composition was prepared by replacing 20 parts by weight of salmon egg extract in example 1 of the present application with 20 parts by weight of hydrolyzed fish eggs.
Comparative example 10
The fermentation complex of comparative example 10 was fermented with only Schizosaccharomyces pombe (without adding Acetobacter xylinum seed solution, the inoculum size of the Schizosaccharomyces pombe seed solution was 2%), and the preparation method of the fermentation complex, the mass ratio of the fermentation complex, salmon egg extract and sturgeon roe paste extract was the same as in example 1, to prepare a tea and caviar composition.
Comparative example 11
The fermentation complex in comparative example 11 was fermented with only Acetobacter xylinum (no schizosaccharomyces pombe seed solution was added, the inoculum size of the Acetobacter xylinum seed solution was 2%), and the remaining fermentation complex was prepared by the preparation method of the Acetobacter xylinum seed solution, and the mass ratio of the fermentation complex, salmon egg extract and sturgeon roe paste extract was the same as in example 1, to prepare a composition.
Test case one, DPPH inhibition test
DPPH is also called 1, 1-diphenyl-2-trinitrophenylhydrazine, has single electron, has strong absorption at 517nm, and has purple characteristic of absolute ethanol solution. When there is a radical in one system, the radical can be captured by the unpaired electrons of DPPH after addition of DPPH, and the color changes from dark purple to pale yellow or colorless after reaction, and the degree of discoloration is quantitatively related to the number of electrons it receives. Thus, the ability of the sample to scavenge free radicals can be judged by DPPH. The higher the DPPH inhibition rate, the better the antioxidation effect of the sample.
The test was performed according to the cosmetic-free radical (DPPH) scavenging test method (T/SHRH 006-2018).
Test sample: the compositions of examples 1-9, comparative examples 1-11 were diluted with deionized water to a 1% by mass aqueous solution.
The test results are shown in table 3 below:
TABLE 3 Table 3
The results of the tests showed that the antioxidant properties of the tea and caviar compositions of examples 1-9 were superior to those of the compositions of comparative examples 1-11. The analyses of example 1 and comparative examples 1 to 6 revealed that there was a synergistic effect between the fermented complex, salmon egg extract, sturgeon roe paste extract, and that the antioxidant effect of the resulting composition was poor, lacking one of the components, or adding only one of the fermented complex, salmon egg extract, sturgeon roe paste extract.
Further, it is evident from comparison of comparative examples 4 to 6 that the effect of comparative example 4 on DPPH is best, and it is revealed that the fermentation complex has the greatest contribution to the effect of DPPH on the basis of the synergistic effect of the fermentation complex, salmon egg extract, sturgeon roe paste extract.
As is clear from comparative examples 1, 4 to 7 and comparative examples 10 and 11, the preparation process of the fermentation complex of the present invention affects the synergistic effect among the fermentation complex, salmon egg extract and sturgeon roe paste extract and affects the antioxidant effect of the final product, in the present invention, in the preparation process of the fermentation complex, preferably, yeast and Acetobacter xylinum are fermented together, the inoculation mass ratio of the yeast seed solution to the Acetobacter xylinum seed solution is 2-5:1, the carbon source is at least one of sucrose, glucose and fructose, and the nitrogen source is at least one of soybean peptone, tryptone and yeast extract.
Test case two, repair Performance test
Cell migration experiments
Experimental principle:
the ability of a cell to migrate directly reflects the level of activity of the cell, and if the cell is able to proliferate and migrate rapidly, a protective mechanism can be established as soon as possible in response to exogenous stimuli, thereby repairing damaged cells. The cell scratch (repair) method is a simple method for measuring the migration movement and repair capability of cells, and is similar to an in-vitro wound healing model, on a single-layer adherent cell cultured on an in-vitro culture dish or a flat plate, a trace gun head or other hard objects are used for scribing a central area where cells grow, the cells in the central part are removed, then the cells are continuously cultured for a set time of experiments, then a cell culture plate is taken out, whether peripheral cells grow (repair) to a central scratch area is observed, so that the repair capability of the cells is judged, a normal control group and an experimental group are usually required to be set in the experiments, the experimental group is a group with certain treatment factors or medicines, exogenous genes and the like, and the migration and repair capability of the cells in each group can be judged through the repair capability of the cells among different groups to the scratch area.
Samples (1) negative control: DMEM medium containing 10% serum; (2) sample group: the tea and caviar compositions of examples 1 to 9, the compositions of comparative examples 1 to 11, were prepared as 2% by mass solutions using a 10% serum DMEM medium as a solvent; (3) positive control: the EGF solution with the mass percent of 10% is prepared by using a DMEM culture solution containing 10% of serum as a solvent.
The experimental steps are as follows: haCaT human keratinocytes were seeded in 12-well plates (100000/well) and cultured in an incubator at 37℃for 24 hours. After the culture is finished, the original culture solution in the holes is discarded, two scratches are marked vertically and horizontally by a gun head in each hole, 100 mu L of DMEM culture solution containing 1% serum is added into a negative control group, 100 mu L of prepared sample solution is added into each hole of a sample group, and 100 mu L of 1% EGF solution is added into each hole of a positive control group, and the culture is carried out for 18 hours. Sampling at the time points of 0 and 18 hours, photographing, randomly selecting 3 areas, and calculating the average value of the scratch areas among cells.
Cell mobility (wound healing rate) was calculated from the average value of the intercellular scratch areas at 0h and 18h, and the repairing effect of the sample was expressed by the cell mobility (wound healing rate), which indicated that the greater the cell mobility (wound healing rate), the better the repairing effect.
Cell mobility (wound healing rate)% = (0 h scratch area-18 h post scratch area)/0 h scratch area
The experimental results are shown in table 4 below:
TABLE 4 Table 4
Compared with a negative control group, the composition of the embodiment and the comparative example can promote migration of HaCaT human keratinocytes and has certain repairing capability. Among them, examples 1 to 3 were superior to comparative examples 1 to 6 in the repairing effect, indicating that the use of the fermentation complex, sturgeon roe paste extract and salmon roe extract in combination in the present application can increase the repairing effect of the final product, lack any of the components thereof, and the repairing effect of the composition is poor.
As is clear from comparison of comparative examples 4 to 6, the repairing effect of comparative example 6 was best, and it was revealed that the salmon egg extract contributed most to the repairing effect on the basis of the synergistic effect of the fermentation complex, sturgeon roe paste extract, and salmon egg extract.
Further, the ratio of the fermentation complex, salmon egg extract and sturgeon roe paste extract affects the repairing effect of the final product, and in this application, the optimal mass ratio of the fermentation complex, salmon egg extract and sturgeon roe paste extract is 3:1:1.
The repair effect of example 1 was superior to comparative examples 7-9, indicating that the replacement of the fermentation complex of the present application, or the replacement of the roe extract with a similar material, resulted in a poor repair effect of the final product.
The repairing effect of example 1 is superior to that of comparative examples 10 to 11, showing that in the present application, the obtained fermentation complex by symbiotic fermentation of yeast and acetobacter xylinum has a better compounding effect with salmon egg extract and sturgeon roe paste extract, and the repairing effect of the composition obtained by compounding by fermentation with a single strain is poor.
Further, the process by which the fermentation complex is fermented also has an impact on the repair effect of the final composition. Comparative examples 1, 4 to 7 show that in the composition of the present application, the optimal inoculation ratio of the merozoite seed solution of Schizosaccharomyces pombe and the Acetobacter xylinum seed solution during the preparation of the fermentation complex is 4:1. the optimal carbon source is sucrose and the optimal nitrogen source is soybean peptone.
Test case three, collagen COL-I mRNA expression level experiment
Experimental principle:
aging is a complex process, and wrinkles are an important evidence and appearance of aging. The dermis is closely related to wrinkle formation and is mainly responsible for the formation of collagen and elastin fibers. Collagen fibers form a dense network to maintain skin cell tissue and resistance, while finer elastin fibers make the skin soft and elastic. The amount of collagen fibers and elastic fibers decreases as the skin ages. The reduction of dermal collagen fibers, disturbance of collagen fiber bundle structure and activation or increase of MMPs caused by ultraviolet rays can lead to decomposition and rupture of collagen, thereby causing the skin to lose elasticity and form wrinkles. Therefore, the expression level of the collagen gene can reflect the collagen content of skin, and then can directly reflect whether the composition has anti-wrinkle capability, thereby reflecting the anti-aging effect.
Samples (1) control group: DMEM medium containing 10% serum; (2) sample group: the tea and caviar compositions of examples 1-9, the compositions of comparative examples 1-11 were formulated as a 2% mass solution using a 10% serum DMEM medium as a solvent.
The experimental steps are as follows: haCaT human keratinocytes were seeded in 6-well plates (200000 cells/well) and cultured in an incubator at 37℃for 24 hours. After the culture is finished, the original culture solution in the holes is discarded, the DMEM culture solution containing 10% of serum is added into the control group, the sample solution is added into each hole of the sample group, and the culture is carried out for 24 hours. After completion of the culture, the supernatant was discarded and 30mJ/cm was used 2 Medium wave Ultraviolet (UVB) irradiation HaCaT human keratinocytes for 30min, wherein the control group was cultured normally without uv irradiation. And detecting the expression quantity of the collagen-related gene COL-I by using qRT-PCR technology.
Expression of experimental results: the higher the expression level of COL-I gene, the better the anti-wrinkle ability.
The experimental results are shown in table 5 below:
TABLE 5
| Group of | COL-I mRNA expression level |
| Control group | 1.04 |
| Example 1 | 3.43 |
| Example 2 | 3.28 |
| Example 3 | 3.06 |
| Example 4 | 3.14 |
| Example 5 | 3.33 |
| Example 6 | 3.26 |
| Example 7 | 3.02 |
| Example 8 | 2.88 |
| Example 9 | 3.25 |
| Comparative example 1 | 1.86 |
| Comparative example 2 | 2.31 |
| Comparative example 3 | 2.18 |
| Comparative example 4 | 1.57 |
| Comparative example 5 | 1.34 |
| Comparative example 6 | 1.47 |
| Comparative example 7 | 1.90 |
| Comparative example 8 | 2.35 |
| Comparative example 9 | 2.13 |
| Comparative example 10 | 2.46 |
| Comparative example 11 | 2.07 |
The test results show that: the compositions of the examples and comparative examples herein both promote the expression of the COL-I gene in HaCaT human keratinocytes, demonstrating the anti-wrinkle ability of the compositions of the examples and comparative examples herein.
Among them, examples 1 to 3 were superior to comparative examples 1 to 6 in anti-wrinkle effect, indicating that the use of the fermentation complex, sturgeon roe paste extract and salmon roe extract in combination in the present application can increase the anti-wrinkle effect of the final product, and the composition was inferior in anti-wrinkle effect in the absence of any one of the components thereof. Further, the ratio of the fermentation complex, the sturgeon roe paste extract and the salmon roe extract affects the anti-wrinkle effect of the final product, and in this application, the optimal mass ratio of the fermentation complex, the salmon roe extract and the sturgeon roe paste extract is 3:1:1.
The repair effect of example 1 was superior to comparative examples 7-9, demonstrating that the replacement of the fermentation complex of the present application, or the replacement of the roe extract with a similar material, resulted in a final product with poor anti-wrinkle effect.
The repairing effect of example 1 is superior to that of comparative examples 10 to 11, showing that in the present application, the obtained fermentation complex by symbiotic fermentation of yeast and acetobacter xylinum has a better compounding effect with salmon egg extract and sturgeon roe paste extract, fermentation is performed by a single strain, and the anti-wrinkle effect of the compounded composition is poor.
Further, the process by which the fermentation complex is fermented also has an effect on the anti-wrinkle effect of the final composition. Comparative examples 1, 4 to 7 show that in the composition of the present application, the optimal inoculation ratio of the merozoite seed solution of Schizosaccharomyces pombe and the Acetobacter xylinum seed solution during the preparation of the fermentation complex is 4:1. the optimal carbon source is sucrose and the optimal nitrogen source is soybean peptone.
Test case four, MMP-1mRNA expression level experiment
Experimental principle:
one of the main causes of skin aging is the altered structure of the dermis layer, which is responsible for many of the causes. External factors activate Matrix Metalloproteinases (MMPs) in vivo, which cause excessive degradation of collagen and elastin supporting skin structures in dermis, and thus cause aging symptoms such as wrinkles, reduced elasticity, etc. of skin. Degradation of the extracellular matrix is largely dependent on proteolytic enzymes, while MMPs are the most important group of proteolytic enzymes, of which MMP-1 is the most predominant enzyme for degradation of type i and type iii collagens. When mRNA of MMP-1 is over-expressed, the content of MMPs is higher, and the MMPs can specifically degrade extracellular matrix components and destroy the normal structures of collagen fibers and elastic fibers, so that aging manifestations such as wrinkles and the like of skin are caused.
MMP-1mRNA testing method:
samples (1) control group: DMEM medium containing 10% serum; (2) sample group: the tea and caviar compositions of examples 1 to 9, the compositions of comparative examples 1 to 11 were prepared as 2% by mass solutions using a 10% serum DMEM medium as a solvent.
The experimental steps are as follows: haCaT human keratinocytes were seeded in 6-well plates (200000 cells/well) and cultured in an incubator at 37℃for 24 hours. After the completion of the culture, the wells were discardedThe original culture solution, the control group and the DMEM culture solution containing 10% of serum are added, the sample solution is added into each hole of the sample group, and the culture is carried out for 24 hours. After completion of the culture, the supernatant was discarded and 30mJ/cm was used 2 Medium wave Ultraviolet (UVB) irradiates HaCaT human keratinocytes for 1h. And detecting the expression quantity of the collagen-related gene MMP-1 by using qRT-PCR technology.
Detecting the index: the higher the level of MMP-1mRNA expression, the more MMP-1, the more easily the collagen fibers and elastic fibers of the skin are destroyed, and the more easily the skin is wrinkled and aged. The results are shown in Table 6.
TABLE 6
| Group of | MMP-1mRNA expression level |
| Control group | 3.55 |
| Example 1 | 2.07 |
| Example 2 | 2.26 |
| Example 3 | 2.18 |
| Example 4 | 2.15 |
| Example 5 | 2.11 |
| Example 6 | 2.13 |
| Example 7 | 2.20 |
| Example 8 | 2.38 |
| Example 9 | 2.21 |
| Comparative example 1 | 2.55 |
| Comparative example 2 | 2.98 |
| Comparative example 3 | 2.76 |
| Comparative example 4 | 2.84 |
| Comparative example 5 | 2.32 |
| Comparative example 6 | 2.96 |
| Comparative example 7 | 2.62 |
| Comparative example 8 | 2.85 |
| Comparative example 9 | 2.93 |
| Comparative example 10 | 2.56 |
| Comparative example 11 | 2.63 |
Compared with a control group, the compositions of the embodiment and the comparative example can inhibit the expression of MMP-1mRNA, can slow down the damage of collagen fiber and elastic fiber by MMP-1, and can slow down the aging of skin.
Comparative example 1, comparative examples 1 to 6, in which the MMP-1mRNA expression level of example 1 was lower than that of comparative examples 1 to 6, showed that the synergistic effect of the fermentation complex, salmon egg extract, sturgeon roe paste extract was present in the composition of the present application, and that the inhibition of MMP-1mRNA by the composition obtained by the addition of one of the components, or by the fermentation complex, salmon egg extract, sturgeon roe paste extract alone was poor.
Further, it is understood from comparative examples 4 to 6 that the inhibition of MMP-1mRNA expression was stronger when sturgeon roe paste extract was used alone, on the basis of the synergistic effect among the fermentation complex, salmon roe extract, and sturgeon roe paste extract, indicating that the contribution of sturgeon roe paste extract to the inhibition of MMP-1mRNA expression was greater. Comparative examples 1, examples 4 to 7, comparative examples 10 and 11 show that the obtained composition has a large influence on MMP-1mRNA expression when the fermentation complex is fermented with only one bacterium, that the obtained tea and caviar composition has a weak inhibitory effect on MMP-1mRNA expression when the fermentation complex is fermented with only one bacterium, and that the carbon source nitrogen source species have a small influence on the inhibitory effect on MMP-1mRNA expression of the final tea and caviar composition when the fermentation complex is fermented with two bacteria, such as the inoculation ratio of yeast and Acetobacter xylinum, and that the schizosaccharomyces pombe seed liquid: when the inoculation ratio of the acetobacter xylinum seed solution is 4:1, the carbon source is sucrose, and the nitrogen source is soybean peptone, the final product has the strongest inhibition effect on MMP-1mRNA expression.
Finally, it should be noted that the above embodiments are only for illustrating the technical solutions of the present application and not for limiting the scope of protection of the present application, and although the present application has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made to the technical solutions of the present application without departing from the spirit and scope of the technical solutions of the present application.
Claims (10)
1. A tea and caviar composition having repairing, antioxidant and anti-wrinkle tightening effects, characterized in that the composition comprises a fermentation complex, salmon egg extract and sturgeon caviar extract;
the fermentation compound is obtained by fermenting saccharomycetes, acetobacter xylinum and black tea.
2. The tea and caviar composition according to claim 1, wherein the mass ratio of the fermentation complex, salmon egg extract and sturgeon caviar extract is (2-4): (0.8-1.2), namely (0.8-1.2); preferably, the mass ratio of the fermentation complex, salmon egg extract and sturgeon roe sauce extract is 3:1:1.
3. The tea and caviar composition of claim 1, wherein the yeast is schizosaccharomyces pombe.
4. The tea and caviar composition of claim 1, wherein the process of preparing the fermentation complex comprises the steps of:
inoculating the saccharomycete seed liquid and the acetobacter xylinum seed liquid into a culture medium containing black tea powder for fermentation culture to obtain the fermentation compound.
5. The tea and caviar composition according to claim 4, wherein the total inoculum size of the yeast seed liquid and the acetobacter xylinum seed liquid is 1 to 3%, and the mass ratio of the yeast seed liquid to the acetobacter xylinum seed liquid is (2 to 5): 1.
6. a tea and caviar composition according to claim 4, wherein the black tea powder-containing medium comprises the following components in mass percent:
0.5-2% of black tea powder, 2-5% of carbon source, 0.5-2% of nitrogen source and the balance of water;
the carbon source comprises at least one of sucrose, glucose and fructose;
the nitrogen source comprises at least one of soybean peptone, tryptone and yeast extract.
7. The tea and caviar composition of claim 4, wherein the fermentation culture conditions include: the temperature of the fermentation culture is 28-35 ℃, the time of the fermentation culture is 56-84 hours, and the ventilation amount of the sterile air is 0.2-0.5 vvm.
8. The tea and caviar composition of claim 4, wherein the method of preparing a fermentation complex further comprises a post-treatment step comprising high pressure homogenization, filtration and sterilization;
the pressure of the high-pressure homogenization is 8-20 MPa, and the time of the high-pressure homogenization is 20-60 min;
the temperature of the sterilization treatment is 85-105 ℃, and the time of the sterilization treatment is more than 15 min.
9. The tea and caviar composition of claim 4, wherein the yeast seed solution is prepared by:
1) Inoculating the bacterial suspension of the saccharomycetes into a solid culture medium A for activation culture, wherein the temperature of the activation culture is 28-33 ℃, and the time of the activation culture is 24-48 hours, so as to obtain activated saccharomycetes;
2) Inoculating the activated saccharomycetes into a liquid culture medium A for expansion culture at 28-33 ℃ for 24-48 hours to obtain saccharomycetes seed liquid;
the solid culture medium A comprises at least one of YM solid culture medium, wort solid culture medium and potato glucose solid culture medium;
the liquid culture medium A comprises at least one of YM liquid culture medium, malt juice liquid culture medium and potato dextrose liquid culture medium;
the preparation method of the acetobacter xylinum seed liquid comprises the following steps:
1) Inoculating the bacterial suspension of acetobacter xylinum into a solid culture medium B for activation culture, wherein the temperature of the activation culture is 28-33 ℃, and the time of the activation culture is 3-5 d, so as to obtain activated acetobacter xylinum;
2) Inoculating the activated acetobacter xylinum into a liquid culture medium B for expansion culture, wherein the expansion culture temperature is 28-33 ℃, and the expansion culture time is 3-5 d, so as to obtain acetobacter xylinum seed liquid;
the solid culture medium B comprises at least one of a yeast glucose chloramphenicol agar culture medium, a wort solid culture medium and a YM solid culture medium;
the liquid culture medium B comprises at least one of yeast glucose chloramphenicol liquid culture medium, malt juice liquid culture medium and YM liquid culture medium.
10. Use of a tea and caviar composition according to any one of claims 1 to 9 in the preparation of a cosmetic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311528473.4A CN117598964A (en) | 2023-11-16 | 2023-11-16 | Tea and caviar composition with repairing, antioxidant, anti-wrinkle and tightening effects and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311528473.4A CN117598964A (en) | 2023-11-16 | 2023-11-16 | Tea and caviar composition with repairing, antioxidant, anti-wrinkle and tightening effects and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117598964A true CN117598964A (en) | 2024-02-27 |
Family
ID=89955487
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311528473.4A Pending CN117598964A (en) | 2023-11-16 | 2023-11-16 | Tea and caviar composition with repairing, antioxidant, anti-wrinkle and tightening effects and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117598964A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994028153A2 (en) * | 1993-05-24 | 1994-12-08 | Nauchno-Proizvodstvennoe Predpriyatie 'farmek' | Low-molecular dna from sturgeon milt, a method of obtaining the dna and a pharmaceutical preparation based on it |
| FR2904550A1 (en) * | 2006-08-03 | 2008-02-08 | Soc Extraction Principes Actif | Use of hydrolysed salmon eggs in cosmetic, dermatological and-or pharmaceutical compositions for increasing melanin synthesis in melanocytes of hair or skin, e.g. in suntan creams and hair lotions |
| JP2014159493A (en) * | 2014-06-12 | 2014-09-04 | Atena:Kk | Sturgeon egg extracts |
| CN108066215A (en) * | 2017-12-27 | 2018-05-25 | 国东启 | A kind of composition of whitening and anti-aging, the skin care item and preparation method for including it |
| CN110101651A (en) * | 2019-05-31 | 2019-08-09 | 广东盛美化妆品有限公司 | Yeast tunning lysis object is split containing two releive repair Essence and preparation method thereof |
| KR20200121059A (en) * | 2019-04-15 | 2020-10-23 | 한국원자력연구원 | Composition for preventing skin damage and preparing method thereof |
| CN113527467A (en) * | 2021-06-21 | 2021-10-22 | 福建省水产研究所（福建水产病害防治中心） | Sturgeon skin collagen polypeptide extraction method, application, cosmetic raw material and preparation method thereof |
| CN115105452A (en) * | 2022-08-23 | 2022-09-27 | 广州优科生物科技有限公司 | Black tea fermentation filtrate and preparation method and application thereof |
| CN116270332A (en) * | 2023-03-27 | 2023-06-23 | 上海伊妮化妆品有限公司 | Anti-wrinkle tightening composition containing sturgeon caviar extract and application thereof |
-
2023
- 2023-11-16 CN CN202311528473.4A patent/CN117598964A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994028153A2 (en) * | 1993-05-24 | 1994-12-08 | Nauchno-Proizvodstvennoe Predpriyatie 'farmek' | Low-molecular dna from sturgeon milt, a method of obtaining the dna and a pharmaceutical preparation based on it |
| FR2904550A1 (en) * | 2006-08-03 | 2008-02-08 | Soc Extraction Principes Actif | Use of hydrolysed salmon eggs in cosmetic, dermatological and-or pharmaceutical compositions for increasing melanin synthesis in melanocytes of hair or skin, e.g. in suntan creams and hair lotions |
| JP2014159493A (en) * | 2014-06-12 | 2014-09-04 | Atena:Kk | Sturgeon egg extracts |
| CN108066215A (en) * | 2017-12-27 | 2018-05-25 | 国东启 | A kind of composition of whitening and anti-aging, the skin care item and preparation method for including it |
| KR20200121059A (en) * | 2019-04-15 | 2020-10-23 | 한국원자력연구원 | Composition for preventing skin damage and preparing method thereof |
| CN110101651A (en) * | 2019-05-31 | 2019-08-09 | 广东盛美化妆品有限公司 | Yeast tunning lysis object is split containing two releive repair Essence and preparation method thereof |
| CN113527467A (en) * | 2021-06-21 | 2021-10-22 | 福建省水产研究所（福建水产病害防治中心） | Sturgeon skin collagen polypeptide extraction method, application, cosmetic raw material and preparation method thereof |
| CN115105452A (en) * | 2022-08-23 | 2022-09-27 | 广州优科生物科技有限公司 | Black tea fermentation filtrate and preparation method and application thereof |
| CN116270332A (en) * | 2023-03-27 | 2023-06-23 | 上海伊妮化妆品有限公司 | Anti-wrinkle tightening composition containing sturgeon caviar extract and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| KYUNG-EUN LEE: "Caviar Extract and Its Constituent DHA Inhibits UVB-Irradiated Skin Aging by Inducing Adiponectin Production", INTERNATIONAL JOURNAL O F MOLECULAR SCIENCES, 31 December 2020 (2020-12-31) * |
| 母姿姿: "鲟鱼子酱对小鼠免疫功能促进作用的研究", 长江大学硕士学位论文, 1 May 2023 (2023-05-01) * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111437237B (en) | Skin care composition with antioxidant, anti-saccharification and anti-aging effects and application thereof | |
| EP2875805B1 (en) | Method for the isolation of glycoprotein-rich fungal extract and its use in anti-ageing cosmetic formulations | |
| CN110960443A (en) | Composition containing recombinant human collagen, freeze-dried material, preparation method of freeze-dried material and cosmetic | |
| CN111603434B (en) | Composition for improving skin wrinkles and cosmetic thereof | |
| CN112494403A (en) | Oat fermentation liquor and preparation method and application thereof | |
| CN110604704A (en) | Peptide composition cosmetic and preparation method thereof | |
| CN111643389A (en) | Anti-wrinkle whitening repair mask containing marine bioactive peptide and preparation method thereof | |
| CN112662718A (en) | Preparation method of oligomeric sodium hyaluronate | |
| CN115054530B (en) | Skin care composition with moisturizing and anti-aging effects and preparation method thereof | |
| CN111228130A (en) | Composition with skin cell energy stimulating and skin barrier repairing effects and preparation method and application thereof | |
| CN115337392A (en) | Recombinant III-type humanized collagen gel and preparation method and application thereof | |
| CN109939019A (en) | A kind of Skin Cell purification cosmetic composition | |
| CN116536368B (en) | Bacillus marinus fermentation product, preparation method and application thereof | |
| CN117598964A (en) | Tea and caviar composition with repairing, antioxidant, anti-wrinkle and tightening effects and application thereof | |
| CN114404344B (en) | Yeast/barley seed fermentation product, product containing same and preparation method and application thereof | |
| CN113604509B (en) | Saccharomyces bifidus fermentation product and preparation method and application thereof | |
| CN110934772A (en) | Wrinkle-removing and anti-aging composition and application thereof in cosmetics | |
| CN114886836A (en) | Freeze-dried powder essence mask for triple repairing of skin barrier and preparation method thereof | |
| CN108969430B (en) | Application of American ginseng fermentation liquor as skin care product or skin care product additive | |
| CN112451438A (en) | Ginseng ferment and polypeptide composition | |
| CN113440455B (en) | Application of highland barley fermentation liquor | |
| CN116942562B (en) | Skin repair water for high-heat skin barrier damage and preparation method thereof | |
| CN113876662B (en) | Skin care composition with anti-aging effect and preparation method and application thereof | |
| CN109453104A (en) | Light spot skin toner Essence and its preparation process | |
| CN115287204B (en) | Kluyveromyces marxianus strain and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |