CN117572001A - High-flux POCT (point of care testing) device and method - Google Patents
High-flux POCT (point of care testing) device and method Download PDFInfo
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- CN117572001A CN117572001A CN202311668769.6A CN202311668769A CN117572001A CN 117572001 A CN117572001 A CN 117572001A CN 202311668769 A CN202311668769 A CN 202311668769A CN 117572001 A CN117572001 A CN 117572001A
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- 238000012123 point-of-care testing Methods 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title abstract description 7
- 238000001514 detection method Methods 0.000 claims abstract description 117
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 23
- 239000003085 diluting agent Substances 0.000 claims abstract description 7
- 239000012898 sample dilution Substances 0.000 claims abstract description 5
- 238000011895 specific detection Methods 0.000 claims abstract description 4
- 238000000018 DNA microarray Methods 0.000 claims description 9
- 238000012360 testing method Methods 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 7
- 230000000694 effects Effects 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims 1
- 238000010790 dilution Methods 0.000 claims 1
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000004737 colorimetric analysis Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000004879 turbidimetry Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010876 biochemical test Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000004848 nephelometry Methods 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses a high-flux POCT detection device and a method, comprising a fixed seat and detection clamping positions, wherein the fixed seat is a revolving body with a rotation center, a plurality of detection clamping positions are fixedly arranged on the revolving body, the detection clamping positions are used for placing detection units, the detection units are used for realizing specific detection single items, and a plurality of detection positions are arranged in a detection device in a circumferentially symmetrical mode, so that a plurality of reagent consumables are parallelly fixed on a tray driven by a motor, and high-flux detection can be realized. The matched detection instrument and consumable are fixed in a special mode, single detection reagent and diluent are preset in the consumable, sample dilution with different proportions can be realized, and the concentration range capable of being tested is expanded.
Description
Technical Field
The invention relates to the technical field of high-flux POCT detection, in particular to a high-flux POCT detection device and method.
Background
The traditional biochemical analyzer adopts a colorimetry and a turbidimetry to analyze the substance content of the blood sample, the colorimetry can detect most blood biochemical test items, and the turbidimetry can detect part of blood immunological test items. The test method is that the serum sample and the reagent are added into a cuvette for reaction at 37 ℃ and detection, the reacted liquid has absorption and scattering effects on monochromatic light with different wavelengths, the transmitted light is weakened, and the content of the detection substance can be obtained through the weakened degree.
In recent years, with the rapid development of microfluidic technology, biochips have been widely used in various fields such as biomedical research, clinical diagnosis, drug development, food sanitation, and environmental monitoring. Many manufacturers have designed single-person multi-item detection instruments and microfluidic biochips.
The test sample of the biochemical analyzer is usually carried by a microfluidic chip, and the problem that the consumable material of the centrifugal microfluidic chip has high cost and can only be used for parallel detection of multiple indexes at present is that the microfluidic chip for parallel detection of multiple indexes is introduced in the integrated device based on multiple detection positions and the analysis method 201810782047.6 of the Chinese patent, but the cost of the consumable material is relatively high and is not cost-effective for single detection.
The invention mainly aims at the high-flux simultaneous detection requirement of single or two items in the market, and provides a high-flux POCT detection device and method for solving the problems.
Disclosure of Invention
The invention aims to provide a high-flux POCT detection device and method, which are used for solving the problems that the current microfluidic chip proposed in the background technology is not cost-effective for single-item detection due to high cost of consumable materials.
In order to achieve the above object, the present invention provides a high throughput POCT detection apparatus, comprising: the fixed seat is a revolution body with a rotation center, a plurality of detection clamping positions are fixedly arranged on the revolution body, the detection clamping positions are used for placing detection units, and the detection units are used for realizing specific detection single items.
As a preferable technical scheme, the rotary body comprises a chassis, a biochip and a chassis cover (4), wherein the chassis cover is fixedly arranged on the inner side of the chassis, the biochip is detachably arranged between the chassis cover and the chassis, and the chassis is connected with an external driving device and is driven to rotate.
As a preferable technical scheme, the detection unit comprises a matched detection instrument and consumable parts, wherein the detection instrument and the consumable parts are fixed in a special mode, and single detection reagent and diluent are preset in the consumable parts.
As a preferred embodiment, the rotor is driven by a centrifugal motor provided therebetween.
The high-flux POCT detection method uses the high-flux POCT detection device, and comprises the following steps:
step S1: the high-flux POCT detection device is installed;
step S2: the fixed seat in the equipment is matched with the consumable material in a special mode, and balancing is achieved;
step S3: through fixing the consumable after the application of sample on same circumference in parallel, realize the parallel detection under the same centrifugal force effect.
As a preferable technical scheme, in the step S2, 1-30 samples are simultaneously placed in the detection units on the revolving body, so that all the detection units are uniformly distributed to achieve dynamic balance.
As a preferable technical scheme, after step S2, different samples are identified by numbering on the device fixing seat, so as to prevent confusion of detection results of the samples.
As a preferred embodiment, step S1 includes:
1) A plurality of detection positions are arranged in the detection equipment in a circumferentially symmetrical mode, and a plurality of reagent consumables are parallelly fixed in a detection unit of the revolving body, so that high-flux detection is realized.
2) The matched detection instrument and consumable are fixed in a special mode, and single detection reagent and diluent are preset in the consumable, so that sample dilution with different proportions is realized, and the concentration range capable of being tested is expanded.
Compared with the prior art, the invention has the beneficial effects that:
the scheme mainly aims at the high-flux simultaneous detection requirement of single items or two items in the market, a plurality of detection positions are set in a circumferentially symmetrical mode in detection equipment, and a plurality of reagent consumables are parallelly fixed on a tray driven by a motor, so that high-flux detection can be realized. The matched detection instrument and consumable are fixed in a special mode, single detection reagent and diluent are preset in the consumable, sample dilution with different proportions can be realized, and the concentration range capable of being tested is expanded.
Drawings
FIG. 1 is a schematic diagram of a high throughput POCT detection device according to the present invention;
FIG. 2 is a flow chart of a high throughput POCT detection method of the present invention;
fig. 3 is a schematic diagram of a detection method of a high throughput POCT detection device according to the present invention.
In the figure: 1-chassis, 2-biochip, 3-collision bead, 4-chassis cover, 5-detection light source, 6-first detection signal receiving device, 7-second detection signal receiving device.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-2, the present invention provides a technical solution: a high throughput POCT detection device, comprising: the fixed seat is a revolution body with a rotation center, a plurality of detection clamping positions are fixedly arranged on the revolution body, the detection clamping positions are used for placing detection units, and the detection units are used for realizing specific detection single items. The revolving body comprises a chassis 1, a biochip 2 and a chassis cover 4, wherein the chassis cover 4 is fixedly arranged on the inner side of the chassis 1, the biochip 2 is detachably arranged between the chassis cover 4 and the chassis 1, and the chassis 1 is connected with an external driving device and is driven to rotate. The detection unit comprises a detection instrument and a consumable matched with each other, the detection instrument and the consumable are fixed in a special mode, and single detection reagent and diluent are preset in the consumable.
Working principle: and (3) a single-detection microfluidic reagent chip, wherein different freeze-dried detection reagents are placed into a reaction detection area in the production process, the surfaces of the microfluidic reagent chips are sealed by a film or a plastic sheet, and the microfluidic reagent chips are packaged in a single sealing way. The microfluidic reagent chip is a disposable consumable for single person use, one reagent chip corresponds to one liquid sample, most typically a peripheral blood (fingertip blood) sample, a user unpacks the sample to be tested from a sample loading hole of the reagent chip, and then the sample enters a sample quantifying area under the drive of centrifugation. Solid impurities in the sample enter the impurity storage area due to the high density, and the quantified sample enters the reaction detection area. The method avoids subjective influence on operation, has small required sample amount, is easy to operate, omits a preparation process of complex operation, and is quick and accurate.
The user can freely match according to the requirement, can carry out the simultaneous detection of single index of multiple samples, also can single sample multiple index detects. During the use, with reagent consumable card in the fixing base of check out test set, can put into 30 detection consumables simultaneously at most on the fixing base, distribute along the circumference. Then, as shown in fig. 3, the liquid irradiated into the detection chamber by the detection light source is received by the first detection signal receiving means by reflection or is received by the second detection signal receiving means by refraction; and the parameters of the sample are known by analyzing the output signals of the first detector and the second detector, such as by colorimetry, turbidimetry and nephelometry.
As a verification scheme, the existing scheme needs 100 microlitres of samples, the detection time is about 10 minutes, and the reagent consumable material is high in price. The invention uses 20 microliter whole blood sample to complete single index detection, shortens the detection time from 10 minutes to 3 minutes, has lower cost for detecting single index, can complete detection of 30 samples in 3 minutes at the same time, and realizes high flux detection.
In summary, the chip structure for detecting single or two indexes by the microfluidic technology can automatically quantify samples, and can detect high-flux micro samples under the drive of a centrifugal motor, so that the cost is low and the use is simple.
(1) The consumable after sample addition is parallelly fixed on the same circumference, so that parallel detection under the action of the same centrifugal force is realized;
(2) The fixed seat in the device is matched with the consumable material in a special mode, 1 to 30 samples can be detected at the same time, and only balancing is needed to achieve dynamic balance (balancing similar to a centrifuge);
(3) Different samples are identified through numbers on the equipment fixing seat, so that confusion of detection results of the samples is prevented;
(4) The high-flux detection can be realized, single-index detection results of 30 samples can be given in 5 minutes, single-sample multi-index detection can be realized, and flexible allocation can be realized.
The scheme mainly aims at the high-flux simultaneous detection requirement of single items or two items in the market, a plurality of detection positions are set in a circumferentially symmetrical mode in detection equipment, and a plurality of reagent consumables are parallelly fixed on a tray driven by a motor, so that high-flux detection can be realized. The matched detection instrument and consumable are fixed in a special mode, single detection reagent and diluent are preset in the consumable, sample dilution with different proportions can be realized, and the concentration range capable of being tested is expanded.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. A high throughput POCT test device, comprising: the fixed seat is a revolving body with a rotation center, a plurality of detection clamping positions are fixedly arranged on the revolving body, the detection clamping positions are used for placing detection units, the detection units are used for realizing specific detection items, a detection light source (5) is arranged above the revolving body, and a first detection signal receiving device (6) and a second detection signal receiving device (7) are respectively arranged at the side and the bottom of the revolving body.
2. The high-throughput POCT detection device of claim 1, wherein the rotary body comprises a chassis (1), a biochip (2) and a chassis cover (4), the chassis cover (4) is fixedly arranged on the inner side of the chassis (1), the biochip (2) is detachably mounted between the chassis cover (4) and the chassis (1), and the chassis (1) is connected with an external driving device and is driven to rotate by the external driving device.
3. The high throughput POCT test apparatus of claim 1, wherein the test unit comprises a test instrument and a consumable which are matched, wherein the test instrument and the consumable are fixed in a special manner, and single test reagents and dilutions are preset in the consumable.
4. The high-throughput POCT cell of claim 1, wherein the rotor is driven by a centrifugal motor disposed therebetween.
5. A high throughput POCT detection method, characterized in that a high throughput POCT detection device as claimed in any of the preceding claims 1 to 3 is used, comprising the steps of:
step S1: the high-flux POCT detection device is installed;
step S2: the fixed seat in the equipment is matched with the consumable material in a special mode, and balancing is achieved;
step S3: through fixing the consumable after the application of sample on same circumference in parallel, realize the parallel detection under the same centrifugal force effect.
6. The high throughput POCT cell of claim 5, wherein in step S2, 1 to 30 samples are simultaneously placed in the detection units on the rotor, so that all the detection units are uniformly distributed to achieve dynamic balance.
7. The high-throughput POCT detection method is characterized in that after step S2, different samples are identified through numbering on a device fixing seat, so that the confusion of detection results of the samples is prevented.
8. A high throughput POCT detection method, wherein step S1 comprises:
1) A plurality of detection positions are arranged in the detection equipment in a circumferentially symmetrical mode, and a plurality of reagent consumables are parallelly fixed in a detection unit of the revolving body, so that high-flux detection is realized.
2) The matched detection instrument and consumable are fixed in a special mode, and single detection reagent and diluent are preset in the consumable, so that sample dilution with different proportions is realized, and the concentration range capable of being tested is expanded.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311668769.6A CN117572001A (en) | 2023-12-05 | 2023-12-05 | High-flux POCT (point of care testing) device and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311668769.6A CN117572001A (en) | 2023-12-05 | 2023-12-05 | High-flux POCT (point of care testing) device and method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117572001A true CN117572001A (en) | 2024-02-20 |
Family
ID=89886244
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311668769.6A Pending CN117572001A (en) | 2023-12-05 | 2023-12-05 | High-flux POCT (point of care testing) device and method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117572001A (en) |
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2023
- 2023-12-05 CN CN202311668769.6A patent/CN117572001A/en active Pending
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