CN117535210A - Salt-tolerant growth-promoting bacterium, microbial inoculum, preparation method of microbial inoculum and application of microbial inoculum - Google Patents
Salt-tolerant growth-promoting bacterium, microbial inoculum, preparation method of microbial inoculum and application of microbial inoculum Download PDFInfo
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- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 claims abstract description 40
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 31
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- 230000001737 promoting effect Effects 0.000 claims abstract description 17
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- 241001645734 Bacillus tropicus Species 0.000 claims abstract description 9
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- 238000009630 liquid culture Methods 0.000 claims description 6
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- 150000007523 nucleic acids Chemical group 0.000 claims description 2
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- 240000004658 Medicago sativa Species 0.000 claims 1
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- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
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- A01N63/22—Bacillus
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Abstract
The application relates to salt-tolerant growth-promoting bacteria, a microbial agent preparation method and application thereof. The salt-resistant growth-promoting bacterium is bacillus tropicalis (Bacillus tropicus), the strain number is YJ33, the preservation number is CCTCC No. M20232194, and the preservation date is: 2023, 11 and 10 days, the preservation address is: eight paths of Lopa nationality mountain in Wuchang district of Wuhan, hubei province, china center for type culture Collection. The salt-tolerant growth-promoting bacteria are obtained by sampling the salt-tolerant growth-promoting bacteria in rhizosphere soil of suaeda salsa in saline-alkali soil and screening and culturing the salt-tolerant growth-promoting bacteria; can be used for preparing alfalfa growth promoting microbial agents. The application of the salt-tolerant growth-promoting bacteria in promoting plant growth under salt stress can obviously improve the salt-tolerant growth-promoting capability of alfalfa, slow down salt damage phenomenon and increase biomass, thereby promoting the growth of alfalfa, improving the physicochemical property of saline-alkali soil to a certain extent, and not only playing a role in increasing yield, but also improving soil.
Description
Technical Field
The application relates to the technical field of microorganisms, in particular to salt-tolerant growth-promoting bacteria, a bacterial agent preparation method and application thereof.
Background
Soil salinization is now an environmental challenge facing the world. Soil salinization can cause low fertility, low and unstable crop yield and brings great obstacle to agricultural production. At present, the grain demands are increasingly high, cultivated land resources are tense, and the method has important significance for solving the problem of saline-alkali soil improvement. The development and utilization of the moderate and mild salinized soil have important significance for the development of agricultural production and the improvement of ecological environment.
Plant growth-promoting bacteria (PGPR) are a large class of bacteria designated to colonize the vicinity of plant root systems, having the function of promoting plant growth, or having the effect of inhibiting plant pathogenic bacteria. PGPR may promote plant growth by improving plant rhizosphere nutrient availability, generating volatile gases, regulating plant hormone levels, and the like through various mechanisms. Currently, more salt tolerant probiotics are the genera Pseudomonas (Pseudomonas) and Bacillus (Bacillus). As an important growth promoting bacterium, the bacillus has the characteristics of high thallus propagation coefficient, good effect, long preparation shelf life, strong stress resistance and the like, and is one of excellent microbial fertilizer strains. The prior art has little research on salt-tolerant growth-promoting microorganisms, and lacks salt-tolerant growth-promoting microorganisms, particularly high-salt-tolerant efficient growth-promoting microorganisms.
Disclosure of Invention
The embodiment of the invention provides a salt-tolerant growth-promoting bacterium, a microbial agent, a preparation method of the microbial agent and application thereof, and at least solves the problems that soil salinization causes low fertility, less research on salt-tolerant growth-promoting microorganisms and the lack of the salt-tolerant growth-promoting microorganisms in the related technology.
The above object of the present application is achieved by the following technical solutions:
in a first aspect, an embodiment of the present application provides a salt-tolerant growth-promoting bacterium, which is characterized in that the salt-tolerant growth-promoting bacterium is bacillus tropicalis (Bacillus tropicus), the strain number is YJ33, the preservation number is cctccc No. M20232194, and the preservation date is: 2023, 11 and 10 days, the preservation address is: eight paths of Lopa nationality mountain in Wuchang district of Wuhan, hubei province, china center for type culture Collection.
According to the embodiment of the invention, the nucleic acid sequence of the salt-tolerant growth-promoting bacteria is shown as SEQ ID NO. 1.
In a second aspect, embodiments of the present application provide a use of a strain of salt tolerant growth promoting bacteria as described in the first aspect to promote plant growth under salt stress.
Further, the plant comprises alfalfa.
In a third aspect, embodiments of the present application provide a use of a strain according to the first aspect for the preparation of a growth-promoting agent.
In a fourth aspect, embodiments of the present application provide a use of a strain according to the first aspect for the preparation of a salt tolerant growth promoting agent.
In a fifth aspect, embodiments of the present application provide a salt tolerant growth promoting bacterial agent comprising a strain of salt tolerant growth promoting bacteria as described in the first aspect.
Further, the active ingredient of the salt-tolerant growth-promoting bacteria agent is bacterial liquid or bacterial cells of the strain of the salt-tolerant growth-promoting bacteria.
In a sixth aspect, embodiments of the present application provide a method for preparing a salt-tolerant growth-promoting microbial agent, where the method includes:
s101, inoculating the strain of the salt-tolerant growth-promoting bacteria into a beef tryptone liquid culture medium;
s102, culturing the inoculated beef tryptone liquid culture medium in a shake flask with the temperature of 27-29 ℃ and the rotating speed of 180r/min for 24 hours to obtain seed liquid;
s103, placing the seed liquid in a centrifugal machine with the temperature of 3-5 ℃ and the rotating speed of 8000 r/min for centrifugal treatment, and collecting the precipitated thalli by hand;
s104, uniformly mixing the precipitate thalli with sterile water to obtain the salt-tolerant growth-promoting microbial agent;
wherein, salt-tolerant growth-promoting bacteria are tropical bacillus (Bacillus tropicus), the strain number is YJ33, the preservation number is CCTCC No. M20232194, and the preservation date is: 2023, 11 and 10 days, the preservation address is: eight paths of Lopa nationality mountain in Wuchang district of Wuhan, hubei province, china center for type culture Collection.
The embodiment of the invention has the beneficial effects that:
the salt-tolerant growth-promoting bacteria provided by the embodiment of the invention can be prepared into a salt-tolerant growth-promoting bacteria agent, and the strain of the salt-tolerant growth-promoting bacteria can be obtained by screening from the plant suaeda salsa rhizosphere soil in the saline-alkali soil, and the acquisition method is simple and convenient; the genetic characteristic and the physiological and biochemical characteristics of the strain are measured, the strain is determined to be bacillus tropicalis (Bacillus tropicus), biological characteristic analysis such as salt tolerance is carried out on the strain, and the strain can still keep activity under the salt concentration of 900 mmol/L.
The salt-tolerant growth-promoting bacteria agent is used for testing the salt-tolerant growth-promoting effect of the alfalfa in a potting experiment under the salt stress condition, and the result shows that the plant height and the total biomass of the alfalfa after the bacteria agent is added are respectively 32.52 percent and 39.06 percent higher than those of the alfalfa which is not subjected to bacteria inoculation treatment. The application of the salt-tolerant growth-promoting microbial inoculum can obviously improve the salt-tolerant growth-promoting capability of the alfalfa under salt stress, slow down the salt damage phenomenon and increase the biomass, thereby promoting the growth of the alfalfa, improving the physicochemical property of the saline-alkali soil to a certain extent, and not only playing a role in increasing the yield, but also improving the soil.
The details of one or more embodiments of the invention are set forth in the accompanying drawings and the description below to provide a more thorough understanding of the other features, objects, and advantages of the invention.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It is evident that the drawings in the following description are only some embodiments of the invention, from which other embodiments can be obtained for a person skilled in the art without inventive effort.
FIG. 1 is a colony morphology of strain YJ33 provided in an example of the present invention;
FIG. 2 is a phylogenetic tree of strain YJ33 provided by an embodiment of the present invention;
FIG. 3 is a graph showing the salt tolerance of strain YJ33 according to the example of the present invention;
FIG. 4 shows the effect of the strain YJ33 on promoting alfalfa growth under salt stress according to the embodiment of the invention;
FIG. 5 is a schematic diagram of plant height of alfalfa under different salt concentrations according to an embodiment of the present invention;
FIG. 6 is a schematic diagram of biomass of alfalfa at different salt concentrations according to an embodiment of the present invention.
In the figure, CS1 is a control group with a salt concentration gradient of 75mmol/L, CS2 is a control group with a salt concentration gradient of 150 mmol/L, CS3 is a control group with a salt concentration gradient of 225 mmol/L, and CS4 is a control group with a salt concentration gradient of 300 mmol/L; BS1 is a test group with a salt concentration gradient of 75mmol/L, BS2 is a test group with a salt concentration gradient of 150 mmol/L, BS3 is a test group with a salt concentration gradient of 225 mmol/L, and BS4 is a test group with a salt concentration gradient of 300 mmol/L.
Detailed Description
Embodiments of the present embodiment will be described in more detail below with reference to the accompanying drawings. While certain embodiments of the present embodiments are illustrated in the accompanying drawings, it is to be understood that the present embodiments may be embodied in various forms and should not be construed as limited to the embodiments set forth herein, but rather are provided to provide a more thorough and complete understanding of the present embodiments. It should be understood that the drawings and the embodiments of the present embodiments are presented for purposes of illustration only and are not intended to limit the scope of the embodiments.
In the embodiment of the invention, the strain of the salt-tolerant growth-promoting bacteria is simply called strain YJ33. The salt-tolerant growth-promoting bacteria provided by the embodiment of the invention can be mixed with an organic fertilizer for application, can be independently applied, and can be used as a base fertilizer and a base fertilizer or can be used as an additional fertilizer.
The salt-resistant growth-promoting bacterium provided by the embodiment of the invention is bacillus tropicalis (Bacillus tropicus), the strain number is YJ33, the preservation number is CCTCC No. M20232194, and the preservation date is as follows: 2023, 11 and 10 days, the preservation address is: eight paths of Lopa nationality mountain in Wuchang district of Wuhan, hubei province, china center for type culture Collection.
Example 1 isolation screening of Strain YJ33
Sampling in saline-alkali soil in the coastal state, selecting rhizosphere soil of suaeda salsa in the saline-alkali soil, and filling the rhizosphere soil into a sterile sampling bag for preservation. Thereafter, the selected rhizosphere soil is diluted to different concentrations to obtain a soil suspension. The soil suspension is respectively coated in NA culture mediums with different NaCl concentrations for screening culture, the culture is carried out for 2 d-3 d at the temperature of 29-31 ℃, the production condition of the strain under different salt concentrations is observed, dominant bacterial groups in the culture mediums are selected for repeated plate streak purification, and a single salt-tolerant growth-promoting bacterial strain YJ33 is obtained, and the salt-tolerant growth-promoting bacterial strain YJ33 is shown in figure 1.
Based on the results shown in FIG. 1, the strain YJ33 in this example grew rapidly and had a short period, and after 24-h culture, the colonies appeared milky white, smooth and opaque in surface, flat, and irregular in edge.
In this embodiment, the selected rhizosphere soil may be diluted to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 And the like.
Example 2 identification of Strain YJ33
DNA was extracted from the single salt-tolerant growth-promoting bacterial strain YJ33 obtained in example 1 and used as a template, a 16SrDNA nucleotide fragment was amplified using a 16SrRNA universal primer as a primer, the amplified fragment was directly subjected to sequence determination, and the sequencing results were aligned. In this embodiment, the sequencing result may be input to a relevant comparison program for comparing the sequencing result. For example, the sequencing result alignment program may include a search tool (Basic Local Alignment Search Tool, BLAST) based on a local alignment algorithm, or the like.
The results show that: the 16SrRNA nucleotide sequence of the strain YJ33 has the highest homology with the 16s rRNA sequence of Bacillus tropicus in bacillus in GenBank gene library, and the homology rate reaches 92%. As shown in FIG. 2 and SEQ ID NO. 1: the result of genetic evolution analysis of the 16SrDNA sequence of Bacillus existing in Genbank by DNAMAN 6.0 shows that the homology of the strain YJ33 16S rRNA with Bacillus tropicus is highest, so that the strain can be primarily judged as the tropical bacillus strain YJ33 of Bacillus.
In this example, the nucleotide sequence of 16SrRNA of strain YJ33 is shown in SEQ ID NO.1,
GGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAATGGATTAAGAGCTTGCTCTTATGAAGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCCATAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATAACATTTTGAACCGCATGGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGTCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGAGACTTGAGTGCAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGAGGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGAAGTTAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACCCTAGAGATAGGGCTTCTCCTTCGGGAGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCATCATTAAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACGGTACAAAGAGCTGCAAGACCGCGAGGTGGAGCTAATCTCATAAAACCGTTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGT
EXAMPLE 3 salt tolerance test of Strain YJ33
Preparing a single salt-tolerant growth-promoting bacterial strain YJ33 obtained in the example 1 into seed solutions, respectively inoculating 2% of the seed solutions into liquid culture media with different salt concentrations, performing shake culture for 24 hours at the temperature of 28 ℃ and the rotating speed of 180r/min, measuring the OD value of the seed solutions, and determining the salt tolerance of the salt-tolerant growth-promoting bacterial strain. A salt tolerance graph as shown in FIG. 3 was obtained, in which the ordinate represents the bacterial concentration (OD 600) and the abscissa represents the salt concentration.
In this embodiment, the liquid culture medium comprises the following components in percentage by weight: beef extract 3%, peptone 5%, distilled water 1000ml, ph=7.2-7.4, wherein,% represents g/1000 mL.
As shown in FIG. 3, the strain YJ33 still maintains a high cell concentration at a salt concentration of 900 mmol/L.
The results of FIG. 3 show that the salt-tolerant growth-promoting bacterial strain YJ33 has a wider application range to NaCl, and can still maintain higher thallus concentration under the condition that the salt concentration reaches 900 mmol/L.
EXAMPLE 4 preparation of salt-tolerant growth-promoting microbial agent
In this embodiment, taking the salt tolerant growth promoting agent as an example, the preparation method of the salt tolerant growth promoting agent for alfalfa includes the following steps S101 to S104.
S101, inoculating a strain of the salt-tolerant growth-promoting bacteria to a culture medium for culture to obtain a colony of the salt-tolerant growth-promoting bacteria.
In this example, the medium may include beef tryptone liquid and NA solid medium. In the culture medium, the strain of the salt-tolerant growth-promoting bacteria is cultivated for 48 hours at the constant temperature of 30 ℃ for standby.
S102, culturing salt-tolerant growth-promoting bacterial colonies in a shake flask at a temperature of 27-29 ℃ and a rotating speed of 180r/min for 24 hours to obtain seed liquid.
In this example, single salt tolerant growth promoting bacterial colonies can be selected, placed in a 100mL Erlenmeyer flask containing 50mL NB liquid medium, and placed in a controlled Wen Yaochuang shake culture for 24 hours. Wherein the temperature of the temperature-controlled shaking table is 27-29 ℃ and the rotating speed is 180 r/min.
S103, placing the seed liquid in a centrifugal machine with the temperature of 3-5 ℃ and the rotating speed of 8000 r/min for centrifugal treatment, and collecting the precipitated thalli by hand.
In this example, the seed solution was inoculated into a 500mL Erlenmeyer flask containing 250mL of NB liquid medium at an amount of 2%, subjected to shaking culture at 28℃and 180r/min for 24h, and centrifuged at 8000 r/min at 3℃to 5℃for 10 min, and the supernatant was discarded to collect the precipitated cells.
S104, uniformly mixing the precipitated thalli with sterile water to obtain the alfalfa salt-tolerant growth-promoting microbial agent.
In this example, the OD of the alfalfa salt-tolerant growth-promoting microbial agent 600 The value is 1.99 to 2.01. In the alfalfa salt-tolerant growth-promoting microbial agent, the effective viable count of the strain YJ33 is more than 102 hundred million cfu/mL.
In an alternative embodiment, the active ingredient of the alfalfa salt-tolerant growth-promoting bacteria agent is a bacterial liquid or a bacterial body of a salt-tolerant growth-promoting bacterial strain.
Example 5 Effect of Strain YJ33 on alfalfa growth under salt stress
Clean alfalfa seeds which are consistent in size, mature, full and free from insect attack are selected to sprout in an incubator. Internal culture conditions: photoperiod 16 h light treatment, 8h dark treatment; the temperature during light treatment is 28 ℃ and the temperature during dark treatment is 22 ℃; the humidity is 50% -80%.
After the alfalfa seeds germinate, transplanting the alfalfa leaves into a plastic flowerpot after the alfalfa leaves are completely unfolded, and placing the mixed nutrient soil into the flowerpot to sterilize at high temperature in a sterilizing pot. In this embodiment, the mixed nutrient soil may include nutrient soil, vermiculite and perlite. Further, the ratio of nutrient soil, vermiculite and perlite may be 4:1:1.
salt treatments were performed at different concentrations, and a total of 4 salt concentration gradients (75 mmol/L, 150 mmol/L, 225 mmol/L, 300 mmol/L) were set.
And (3) respectively pouring the saline solution 50mL with the 4 salt concentration gradients into each pot of alfalfa, transplanting the alfalfa seedlings with consistent growth vigor after the solution is completely absorbed, planting 15 plants in each pot, thinning the alfalfa seedlings after the first multiple leaves of the alfalfa seedlings are completely unfolded, and ensuring the uniform size of the alfalfa seedlings in each pot.
Treatment of test group: OD of 50mL was applied once each on day 21, day 28 and day 35 of alfalfa seedling engrafting into the pot 600 2.0 of alfalfa salt-tolerant growth-promoting microbial agent;
treatment of control group: sterile water was poured once each at day 21, day 28 and day 35, respectively, of alfalfa seedlings into the pots, for 50 mL.
In this example, the growth conditions of alfalfa seedlings in the alfalfa salt-tolerant probiotic groups and the control group are shown in FIG. 4.
The plant height of the plant is measured after the last alfalfa salt-tolerant growth-promoting microbial agent and sterile water are irrigated for 7 days as shown in figure 5, and the indexes such as total biomass of the plant is measured after the last alfalfa salt-tolerant growth-promoting microbial agent and sterile water are irrigated for 7 days as shown in figure 6.
The results show that the plant height and the total biomass of the alfalfa after the microbial inoculum is added are respectively 32.52 percent and 39.06 percent higher than those of the alfalfa which is not inoculated with the microbial inoculum. The application of the alfalfa salt-tolerant growth-promoting microbial inoculum can obviously improve the salt-tolerant growth-promoting capability of the alfalfa under salt stress, slow down salt damage phenomenon and increase biomass, thereby promoting the growth of the alfalfa, improving the physicochemical property of the saline-alkali soil to a certain extent, and not only playing a role in increasing yield, but also improving the soil.
It should be noted that the term "comprising" and its variants as used in the embodiments of the present invention are open-ended, i.e. "including but not limited to". The term "based on" is based at least in part on. The term "one embodiment" means "at least one embodiment"; the term "another embodiment" means "at least one additional embodiment"; the term "some embodiments" means "at least some embodiments. References to "one or more" modifications in the examples of the invention are intended to be illustrative rather than limiting, and it will be understood by those skilled in the art that "one or more" is intended to be interpreted as "one or more" unless the context clearly indicates otherwise.
User information (including but not limited to user equipment information, user personal information and the like) and data (including but not limited to data used for analysis, stored data, displayed data and the like) according to the embodiment of the invention are information and data which are allowed by a user or are fully allowed by all parties, and the collection, the use and the processing of related data are required to comply with related laws and regulations and standards of related countries and regions, and corresponding operation entrances are provided for the user to select to allow or reject.
The steps described in the method embodiments provided in the embodiments of the present invention may be performed in a different order and/or performed in parallel. Furthermore, method embodiments may include additional steps and/or omit performing the illustrated steps. The scope of the invention is not limited in this respect.
The term "embodiment" in this specification means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the invention. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive. The various embodiments in this specification are described in a related manner, with identical and similar parts being referred to each other. In particular, for apparatus, devices, system embodiments, the description is relatively simple as it is substantially similar to method embodiments, see for relevant part of the description of method embodiments.
The above examples merely represent a few embodiments of the present invention, which are described in more detail and are not to be construed as limiting the scope of the patent claims. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of the invention should be assessed as that of the appended claims.
Claims (9)
1. The salt-tolerant growth-promoting bacterium is characterized in that the salt-tolerant growth-promoting bacterium is bacillus tropicalis (Bacillus tropicus), the strain number is YJ33, the preservation number is CCTCC No. M20232194, and the preservation date is: 2023, 11 and 10 days, the preservation address is: eight paths of Lopa nationality mountain in Wuchang district of Wuhan, hubei province, china center for type culture Collection.
2. The salt tolerant growth-promoting bacterium of claim 1, wherein the nucleic acid sequence of the salt tolerant growth-promoting bacterium is shown in SEQ ID No. 1.
3. Use of a strain of salt tolerant growth promoting bacteria according to claim 1 for promoting plant growth under salt stress.
4. The use according to claim 3, wherein the plant comprises alfalfa.
5. The use of a strain of salt tolerant growth-promoting bacteria according to claim 1 in the preparation of a growth-promoting bacteria agent.
6. The use of a strain of salt tolerant growth-promoting bacteria according to claim 1 in the preparation of a salt tolerant growth-promoting bacteria agent.
7. A salt-tolerant growth-promoting bacterium comprising the strain of salt-tolerant growth-promoting bacterium according to claim 1.
8. The salt-tolerant growth-promoting microbial agent according to claim 7, wherein the active ingredient of the salt-tolerant growth-promoting microbial agent is a bacterial liquid or a bacterial cell of a strain of the salt-tolerant growth-promoting microbial agent.
9. A method for preparing a salt-tolerant growth-promoting microbial agent, which is characterized by comprising the following steps:
s101, inoculating the strain of the salt-tolerant growth-promoting bacteria into a beef tryptone liquid culture medium;
s102, culturing the inoculated beef tryptone liquid culture medium in a shake flask with the temperature of 27-29 ℃ and the rotating speed of 180r/min for 24 hours to obtain seed liquid;
s103, placing the seed liquid in a centrifugal machine with the temperature of 3-5 ℃ and the rotating speed of 8000 r/min for centrifugal treatment, and collecting the precipitated thalli by hand;
s104, uniformly mixing the precipitate thalli with sterile water to obtain the salt-tolerant growth-promoting microbial agent;
wherein, salt-tolerant growth-promoting bacteria are tropical bacillus (Bacillus tropicus), the strain number is YJ33, the preservation number is CCTCC No. M20232194, and the preservation date is: 2023, 11 and 10 days, the preservation address is: eight paths of Lopa nationality mountain in Wuchang district of Wuhan, hubei province, china center for type culture Collection.
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