CN117482016A

CN117482016A - Composition with multi-way acne removing effect and preparation method thereof

Info

Publication number: CN117482016A
Application number: CN202311548401.6A
Authority: CN
Inventors: 张薛勤; 张丽梅; 闫淑萍; 李爱民; 邢岩; 李颖
Original assignee: Guozhen Health Technology Beijing Co ltd
Current assignee: Guozhen Health Technology Beijing Co ltd
Priority date: 2023-11-20
Filing date: 2023-11-20
Publication date: 2024-02-02

Abstract

The invention provides a composition with a multi-way acne removing effect and a preparation method thereof. The acne-removing composition disclosed by the invention plays a role in removing acnes through different ways, and is verified to play a role in five ways of regulating androgen metabolism, resisting endotoxin toxicity, controlling grease generation, eliminating inflammation and inhibiting microbial growth of acne infection, and has a role in inhibiting melanin generation on acne skin, so that recovery of acne muscles is facilitated.

Description

Composition with multi-way acne removing effect and preparation method thereof

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to a composition with a multi-way acne removing effect and a preparation method thereof.

Background

The medical name of the acne is acne, which is an inflammatory disease caused by sebaceous gland grease secretion and microorganism breeding. Currently, the technologies for acne removal are divided into surgical treatment, physical treatment and drug treatment.

Surgical treatment prevents the infection and further deterioration of vaccinia by thoroughly squeezing out microorganisms, microorganism secretions and lipid metabolites in hair follicles by means of an instrument through a sterile manipulation technique. The operation treatment speed is high, but the pain is strong, the recurrence is easy, and if pathogenic bacteria enter blood vessels in the process, the serious result is caused, so the risk is high.

The physical therapy kills microorganisms such as propionibacterium acnes and the like which induce acnes by utilizing the specific wavelength of laser, has low risk of physical laser therapy and can induce regeneration of collagen cells, and is popular for many people at present, but the physical laser therapy only plays a role in sterilization and cannot solve more problems, so that the problems are easy to relapse, and in addition, the phenomena such as pain and swelling of skin, pigmentation and the like can be caused by laser acne removal. Mishandling of laser acne treatment can also cause a series of injuries to the skin.

The medicine treatment is divided into antibiotics, anti-inflammatory medicines, medicines for inhibiting oil secretion and the like according to different action ways, and the main purpose of the medicine treatment is to inhibit oil secretion and microbial growth of skin, and a plurality of acne-removing products such as acne-removing ointment, acne-removing essential oil, acne-removing essence, acne-removing face cream and the like are available on the market. The drug treatment needs to be selected according to different situations of individuals, if the single drug can not achieve the effect or the effect is not long, a certain professional foundation is needed depending on the combination drug, and the acne removal effect is not achieved as same as the single drug when the combination drug is selected.

CN103462870a discloses a dandelion honeysuckle acne-removing mask and a preparation method thereof, wherein the mask is prepared from the following raw materials: 80-100 g of dandelion, 20-30 g of honeysuckle, 30-50 g of mung bean and 10-15 g of honey; the preparation method comprises decocting herba Taraxaci and flos Lonicerae in water, filtering to obtain 500-600 ml filtrate, adding semen Phaseoli Radiati, decocting in water for 1-3 hr, and adding Mel. The dandelion has the effects of clearing heat and detoxicating, and broad-spectrum antibiosis, and the honeysuckle has the effects of clearing heat and diminishing inflammation, and can effectively treat acne. The mung bean is matched for enhancing the curative effect of clearing heat and detoxicating; the honey is matched for seasoning, so that the antibacterial and anti-inflammatory effects of dandelion are enhanced, the face of the face-care product directly has the effects of cleaning and sterilizing the skin, and the face-care product also has the nutrition care effect.

CN105267125a discloses a dandelion acne-removing and face-nourishing mask and a preparation method thereof, relates to an acne-removing and face-nourishing mask and a preparation method thereof, and solves the problems that the existing mask has potential safety hazard, is easy to cause serious skin problems and cannot improve the dullness, roughness, looseness and fine lines of the skin. The acne-removing face-beautifying mask is prepared from dandelion, fructus forsythiae, radix angelicae, rose, clove, pearl powder and honey. The method comprises the following steps: 1. pulverizing herba Taraxaci, fructus forsythiae, radix Angelicae Dahuricae, flos Rosae Rugosae, flos Caryophylli and Margarita powder with pulverizer, sieving with 120 mesh sieve, and stirring to obtain mixed medicinal powder; 2. distilled water and honey are slowly added into the mixed medicinal powder, and stirred into paste, so that the dandelion acne-removing and face-nourishing mask is obtained.

The existing acne removing technology is rich and various, and the surgical treatment and the physical treatment have strong action and quick response to the formed acnes, but have great side effects. The medicine has small side effect, but has various action ways, is difficult to select, and has insignificant acne removing effect due to improper selection. Most of the three acne removing technologies are temporary and temporary, and the acne is easy to recur.

Therefore, the acne-removing composition is developed, not only can play roles of controlling oil, inhibiting bacteria and removing acne marks externally, but also can be used for regulating the androgen level, controlling the strive of sebaceous gland function and reducing inflammatory reaction internally, realizes internal and external regulation, recovers acne muscles, is not easy to relapse, and is a research focus in the field.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide a composition with the multi-way acne-removing effect and a preparation method thereof, which not only can play roles of controlling oil, inhibiting bacteria and removing acne marks externally, but also can regulate the androgen level internally, control the strive of sebaceous gland function and reduce inflammatory reaction, thereby realizing internal and external regulation and multi-way strong acne removal.

In order to achieve the aim of the invention, the invention adopts the following technical scheme:

in a first aspect, the invention provides a composition with multi-way acne-removing effect, wherein the raw materials of the composition comprise dandelion extract, honeysuckle extract, sophora flower extract and purslane extract.

The extracts of dandelion, honeysuckle, pagodatree flower and purslane are used as acne removing raw materials, and the obtained composition can play a role in removing acnes through five ways of regulating androgen level, inhibiting sebaceous gland grease secretion, inhibiting the growth of propionibacterium acnes and staphylococcus aureus, resisting endotoxin and resisting inflammation, has a whitening function, and reduces melanin deposition in the later stage of vaccinia development.

Acne (comedo, acne sore) is mainly caused by imbalance of yin and yang of the five viscera, which can lead to acne, wherein heat, dampness, phlegm and blood stasis are the main factors causing acne. In classical prescriptions for treating acne, the materials with bitter taste and cold nature account for the most.

Dandelion is bitter and sweet in taste and cold in nature, and dandelion is used in the medicine formula for treating acne in different syndromes, because dandelion has broad-spectrum bactericidal effect, and is especially suitable for gram-positive suppurative cocci; secondly, the external and internal medicines have anti-inflammatory effects, can inhibit infiltration and proliferation of leucocytes, reduce release of various inflammatory factors, inhibit proliferation of granulation tissues and prevent adhesion and scar formation; thirdly, anti-follicular acarid activity; fourth, the skin is repaired, the flavonoid substances that are rich have very strong therapeutic effects on acne formation and pigmentation and scar tissue after healing, biological synergistic effects of multienzyme and nonenzyme and antibiotic anti-inflammatory and synergistic effects, can block the injury effect of superoxide radical to skin effectively, carotenoid in dandelion can also be converted into vitamin A in vivo directly, promote metabolism of epidermis and horny layer of skin tissue, nutrient substances such as various amino acids contained, can nourish skin, promote metabolism of skin after acne healing, prevent skin pigmentation. Therefore, according to the Chinese and western theory, dandelion has great potential to be used as a core raw material of the acne-removing composition.

The compatibility proportion of dandelion and honeysuckle is extremely high, the research on the acne-removing efficacy of the honeysuckle is supported by more researches, and the honeysuckle is selected as one of the acne-removing combined raw materials.

Excessive damp-heat is often the root cause of acne, and pagodatree flower and purslane have strong dampness-dispelling effect, so pagodatree flower and purslane are selected as one of the combined raw materials for acne removal.

Preferably, the raw materials of the composition include 1-2 parts by weight of dandelion extract, for example, 1.2 parts, 1.4 parts, 1.6 parts, 1.8 parts and the like, 1-2 parts by weight of honeysuckle extract, for example, 1.2 parts, 1.4 parts, 1.6 parts, 1.8 parts and the like, 1-2 parts by weight of sophora flower extract, for example, 1.2 parts, 1.4 parts, 1.6 parts, 1.8 parts and the like, and 1-2 parts by weight of purslane extract, for example, 1.2 parts, 1.4 parts, 1.6 parts, 1.8 parts and the like.

Preferably, the raw materials of the composition further comprise 0.05-0.15 part of cubilose acid, for example, 0.06 part, 0.08 part, 0.1 part, 0.12 part, 0.14 part and the like.

The bird's nest acid has the potential of oral beauty effect, especially whitening effect, and the bird's nest acid is added into the acne removing composition, so that the recovery of the acne skin in the later period is facilitated, and the bird's nest acid has the function of enhancing immunity, so that the self-healing capability of acne of an organism is facilitated.

Preferably, the dandelion extract, the honeysuckle extract, the sophora flower extract and the purslane extract are extracted by water extraction and/or alcohol extraction.

Preferably, the water extraction is carried out by a method comprising: pulverizing plant, extracting with water, ultrasonic extracting, centrifuging, concentrating, and lyophilizing to obtain water extract of plant.

Preferably, the pulverization is carried out to 30 to 50 mesh, for example, 35 mesh, 40 mesh, 45 mesh, etc.

Preferably, the water extraction liquid ratio is 1 (18-22), for example, 1:19, 1:20, 1:21 and the like.

Preferably, the temperature of the water extraction is 75-85 ℃, for example, 76 ℃, 78 ℃,80 ℃, 82 ℃, 84 ℃ and the like, and the time is 0.5-1.5h, for example, 0.6h, 0.8h, 1h, 1.2h, 1.4h and the like.

Preferably, the ultrasonic extraction time is 8-12min, for example, 9min, 10min, 11min, etc.

Preferably, the rotational speed of the centrifugation is 3500-4500rpm, for example, 3600rpm, 3800rpm, 4000rpm, 4200rpm, 4400rpm, etc., and the time is 3-8min, for example, 4min, 5min, 6min, 7min, etc.

Preferably, the concentration is to 1/5 of the original volume.

Preferably, the alcohol extraction is carried out by a process comprising: pulverizing the plant, extracting with alcohol, centrifuging, concentrating, and freeze drying to obtain the plant extract.

Preferably, the alcohol extraction liquid ratio is 1 (12-18), for example, 1:13, 1:14, 1:15, 1:16, 1:17 and the like can be adopted.

Preferably, the extracting agent used for the alcohol extraction is ethanol water solution.

Preferably, the mass percentage of the ethanol in the ethanol water solution is 60-70%, for example, 62%, 64%, 66%, 68% and the like.

Preferably, the temperature of the alcohol extraction is 75-85 ℃, for example, 76 ℃, 78 ℃,80 ℃, 82 ℃, 84 ℃ and the like, and the time is 1.5-2.5 hours, for example, 1.6 hours, 1.8 hours, 2 hours, 2.2 hours, 2.4 hours and the like.

Preferably, the concentration is to 1/5 of the original volume.

Preferably, the composition comprises dandelion alcohol extract, honeysuckle flower alcohol extract, sophora flower alcohol extract, purslane alcohol extract and nidus Collocaliae acid;

preferably, the composition comprises dandelion water extract, honeysuckle water extract, sophora flower alcohol extract, purslane alcohol extract and nidus Collocaliae acid;

preferably, the composition comprises dandelion aqueous extract, honeysuckle aqueous extract, sophora flower aqueous extract, purslane aqueous extract and nidus Collocaliae acid.

In a second aspect, the present invention provides a method for preparing a composition having multi-path acne-removing efficacy according to the first aspect, the method comprising: and uniformly mixing the dandelion extract, the honeysuckle extract, the sophora flower extract and the purslane extract to obtain the composition.

The numerical ranges recited herein include not only the recited point values, but also any point values between the recited numerical ranges that are not recited, and are limited to, and for the sake of brevity, the invention is not intended to be exhaustive of the specific point values that the recited range includes.

Compared with the prior art, the invention has the following beneficial effects:

the acne-removing composition disclosed by the invention plays a role in removing acnes through different ways, and is verified to play a role in five ways of regulating androgen metabolism, resisting endotoxin toxicity, controlling grease generation, eliminating inflammation and inhibiting microbial growth of acne infection, and has a role in inhibiting melanin generation on acne skin, so that recovery of acne muscles is facilitated.

Drawings

FIG. 1 is a graph showing the effect of the composition prepared in example 1 on the NO secretion level of RAW264.7 cells;

FIG. 2 is a graph showing the effect of the composition prepared in example 1 on the lipid content of SZ95 cells.

Detailed Description

The technical scheme of the invention is further described by the following specific embodiments. It will be apparent to those skilled in the art that the examples are merely to aid in understanding the invention and are not to be construed as a specific limitation thereof.

The terms "comprising," "including," "having," "containing," or any other variation thereof, are intended to cover a non-exclusive inclusion. For example, a composition, step, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, step, method, article, or apparatus.

"optional" or "any" means that the subsequently described event or event may or may not occur, and that the description includes both cases where the event occurs and cases where the event does not.

The indefinite articles "a" and "an" preceding an element or component of the invention are not limited to the requirement (i.e. the number of occurrences) of the element or component. Thus, the use of "a" or "an" should be interpreted as including one or at least one, and the singular reference of an element or component includes the plural reference unless the amount clearly dictates otherwise.

The description of the terms "one embodiment," "some embodiments," "exemplarily," "specific examples," or "some examples," etc., herein described means that a specific feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this document, the schematic representations of the above terms are not necessarily for the same embodiment or example.

In the following examples, all reagents and consumables were purchased from the reagent manufacturers routine in the art unless specifically indicated; unless otherwise indicated, all methods and techniques used are those conventional in the art.

Preparation example 1

The preparation example provides a water extraction process, which comprises the following specific steps:

pulverizing plants to 40 meshes, weighing 10g into a conical flask, adding water according to a feed-liquid ratio of 1:20, uniformly mixing, extracting at 80 ℃ for 1h, then carrying out ultrasonic extraction for 10min, filtering, centrifuging at 4000rpm/min for 5min, concentrating the supernatant under reduced pressure to 1/5 of the original volume, and carrying out freeze drying, weighing and preserving to obtain the water extract of the plants.

Preparation example 2

The preparation example provides an alcohol extraction process, which comprises the following specific steps:

pulverizing plants to 40 meshes, weighing 10g into a conical flask, adding 70% ethanol according to a feed-liquid ratio of 1:15, uniformly mixing, extracting at 80 ℃ for 2 hours, filtering, centrifuging at 4000rpm/min for 5 minutes, concentrating the supernatant under reduced pressure to 1/5 of the original volume, and weighing and preserving after freeze drying to obtain the alcoholic extract of the plants.

Preparation example 3

pulverizing plants to 40 meshes, weighing 10g into a conical flask, adding 60% ethanol according to a feed-liquid ratio of 1:20, uniformly mixing, extracting for 2 hours at 85 ℃, filtering, centrifuging at 4000rpm/min for 5 minutes, concentrating the supernatant under reduced pressure to 1/5 of the original volume, and weighing and preserving after freeze drying to obtain the alcoholic extract of the plants.

Example 1

The embodiment provides a composition with multi-way acne removal efficacy, which comprises the following components in parts by weight:

2 parts of dandelion alcohol extract, 1 part of honeysuckle alcohol extract, 2 parts of sophora flower alcohol extract, 2 parts of purslane alcohol extract and 0.12 part of nidus Collocaliae acid;

the raw materials are prepared by adopting the method provided in preparation example 2.

Example 2

1 part of dandelion water extract, 1 part of honeysuckle water extract, 1 part of sophora flower alcohol extract, 1 part of purslane alcohol extract and 0.08 part of nidus Collocaliae acid;

the water extracts of the raw materials are prepared by adopting the method provided by the preparation example 1, and the alcohol extracts are prepared by adopting the method provided by the preparation example 2.

Example 3

2 parts of dandelion water extract, 1 part of honeysuckle water extract, 2 parts of sophora flower water extract, 1 part of purslane water extract and 0.12 part of nidus Collocaliae acid;

the raw materials are prepared by adopting the method provided in preparation example 1.

Example 4

2 parts of dandelion alcohol extract, 1 part of honeysuckle alcohol extract, 2 parts of sophora flower alcohol extract and 2 parts of purslane alcohol extract;

Example 5

The present example provides a composition with multi-way acne-removing effect, which is different from example 1 only in that 3 parts of dandelion alcohol extract is adjusted, and other raw materials, amounts and preparation methods are the same as those of example 1.

Example 6

The present example provides a composition with multi-way acne-removing effect, which is different from example 1 only in that 3 parts of the honeysuckle flower alcohol extract is adjusted, and other raw materials, amounts and preparation methods are the same as those of example 1.

Example 7

This example provides a composition with multi-way acne-removing effect, which is different from example 1 only in that 3 parts of the pagodatree flower alcohol extract is adjusted, and other raw materials, amounts and preparation methods are the same as those of example 1.

Example 8

This example provides a composition with multi-way acne-removing effect, which is different from example 1 only in that the purslane alcohol extract is adjusted to 3 parts, and other raw materials, amounts and preparation methods are the same as in example 1.

Comparative example 1

This comparative example provides a composition with multi-way acne-removing efficacy, which differs from example 1 only in that no dandelion alcohol extract is added, and other raw materials, amounts and preparation methods are the same as in example 1.

Comparative example 2

This comparative example provides a composition with multi-way acne-removing effect, which is different from example 1 only in that no honeysuckle alcohol extract is added, and other raw materials, amounts and preparation methods are the same as example 1.

Comparative example 3

This comparative example provides a composition with multi-way acne-removing effect, which is different from example 1 only in that no pagodatree flower alcohol extract is added, and other raw materials, amounts and preparation methods are the same as in example 1.

Comparative example 4

This comparative example provides a composition with multi-way acne-removing efficacy, which differs from example 1 only in that no purslane alcohol extract is added, and other raw materials, amounts and preparation methods are the same as in example 1.

Test example 1

Inflammatory factor content determination

(1) 2.0 to 3.0X10 ⁵ The cells were inoculated into 96-well plates at a density of/mL RAW264.7 and cultured for 24 hours.

(2) Normal control wells (medium), LPS-induced model wells (medium+1 μg/mL LPS), sample wells containing different concentrations of starting material (medium+1 μg/mL lps+different concentrations of starting material) were set, the supernatant carefully discarded, the corresponding solution was added to each well, and incubated for 24h.

(3) Collecting culture supernatant of each well, centrifuging at 2000r/min for 5min, and collecting supernatant.

(4) According to the procedure of the nitric oxide kit (Beijing Bi Yuntian, S0021S), 50 mu L of culture supernatant is taken from each hole, 50 mu L of reagent 1 is added, then 50 mu L of reagent 2 is added, and OD is measured ₅₄₀ The concentration of NO per well (μm) was calculated from the standard curve.

The results of the NO content (μm) test in each sample are shown in table 1, and the effect of the composition prepared in example 1 on the NO secretion level of RAW264.7 cells is shown in fig. 1, where "×" indicates that there is a very significant difference, P < 0.01, from the normal group; "#" indicates a very significant difference from the model group, P < 0.01; "#" indicates a significant difference from the model group, P < 0.05.

TABLE 1

According to the table data, the dandelion extract, the honeysuckle extract, the sophora flower extract and the purslane extract obtained by the preparation method have good anti-inflammatory effect when being matched for use, and the anti-inflammatory effect is further improved by adding the nidus Collocaliae acid; from examples 1 and 5, it is apparent that when the amount of dandelion extract in the raw materials is too large, the anti-inflammatory effect is reduced; as is clear from examples 1 and 6, when the amount of the honeysuckle extract in the raw materials is too large, the anti-inflammatory effect is lowered; from examples 1 and 7, it is apparent that when the amount of the sophora flower extract in the raw material is too large, the anti-inflammatory effect is lowered; from examples 1 and 8, it is evident that when the amount of purslane extract used in the raw materials is too large, the anti-inflammatory effect is reduced; the anti-inflammatory efficacy decreases in the absence of any plant extract in the composition.

Test example 2

Oil control test

(1) Taking SZ95 cells in the logarithmic phase, and inoculating the SZ95 cells into a 24-well plate;

(2) Setting a normal control group, and setting 4 sample groups with different concentrations in parallel;

(3) 1mg/mL of nile red stock solution, diluted to 10 μg/mL with PBS when used;

(4) Discarding cell supernatant, adding 1mL PBS buffer solution into each well, washing once, adding 0.5mL nile red use solution into each well, and placing the mixture in an incubator for incubation for 10min;

(5) Discarding the nile red use solution, lightly washing the nile red use solution with PBS buffer solution for 3 times, and adding 1mL of PBS buffer solution into each hole;

(6) Fluorescence intensity measurement: using a multifunctional microplate reader to excite the wavelength 552 and the emission wavelength 636 to detect the fluorescence intensity of each well;

(7) And (3) calculating: calculating the mean value and standard deviation of fluorescence intensity F of each group, adopting Excel 2019 software to conduct data analysis, and adopting a mean value plus-minus standard deviation method to represent experimental results, wherein the two groups are compared by adopting Student's t-test, P < 0.05 to judge that the two groups have obvious differences, and P < 0.01 to judge that the two groups have extremely obvious differences.

The results of the cellular lipid fluorescence intensity test are shown in Table 2, and the effect of the composition prepared in example 1 on the lipid content of SZ95 cells is shown in FIG. 2.

TABLE 2

According to the table data, the dandelion extract, the honeysuckle extract, the sophora flower extract and the purslane extract obtained by the preparation method have good oil control effect when being matched for use, and the oil control effect is further improved by adding the nidus Collocaliae acid; from examples 1 and 5, when the amount of dandelion extract in the raw materials is too large, the oil control effect is reduced; as can be seen from examples 1 and 6, when the amount of the honeysuckle extract in the raw materials is too large, the oil control effect is reduced; as can be seen from examples 1 and 7, when the amount of the sophora flower extract in the raw material is too large, the oil control effect is reduced; as can be seen from examples 1 and 8, when the amount of purslane extract in the raw materials is too large, the oil control effect is reduced; the oil control efficacy decreases in the absence of any plant extract in the composition.

Test example 3

Antibacterial test

(1) Activating propionibacterium acnes, staphylococcus aureus and staphylococcus epidermidis, culturing strains by streaking, flushing the strains on agar (about 10mL of a flat plate) with sterile physiological saline to prepare a bacterial suspension, diluting the bacterial suspension 100 times with a culture medium to prepare 5mL of a test solution of working bacteria and 5mL of a double-material liquid culture medium, adding the test solution into the same test tube, wherein the test tube is named as No. 1, 5mL of the test tube is named from the test tube No. 1, and 5mL of the double-material liquid culture medium is added into another test tube, and the test tube is named as No. 2; taking 5mL from a test tube No. 2, adding 5mL of the double-material liquid culture medium into another test tube, naming the test tube No. 3, numbering the test tube No. N, and discarding 5mL from the test tube No. N; taking 100 mu L of each test tube, adding the test tubes into 3 holes of a 96-well plate, designing 3 repeated holes, adding 100 mu L of working bacteria liquid into each hole, and simultaneously setting a positive control tube (sample diluent+double-material culture medium) and a negative control tube (working bacteria liquid+culture medium); the added 96-well plate is covered with a cover, and the corresponding incubator is placed for culture.

(2) The wells were observed for turbidity, recorded, absorbance at 600nm was measured for each well, with "-" indicating turbidity in the medium, indicating strain growth, "±" indicating semi-turbidity in the medium (OD value between negative and positive control wells), indicating inhibition of strain growth, and "+" indicating clarification in the medium, indicating no strain growth.

The results of the antibacterial effect test of the compositions of the examples and the comparative examples on Propionibacterium acnes are shown in Table 3, the antibacterial effect test on Staphylococcus aureus is shown in Table 4, and the antibacterial effect test on Staphylococcus epidermidis is shown in Table 5.

TABLE 3 Table 3

Note that: "-" indicates medium turbidity, "±" indicates medium half turbidity (OD value between negative control well and positive control well), and "+" indicates medium clarity.

TABLE 4 Table 4

TABLE 5

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According to the table data, the dandelion extract, the honeysuckle extract, the pagodatree flower extract and the purslane extract obtained by the preparation method have good antibacterial efficacy (the stronger and better antibacterial effects on propionibacterium acnes and staphylococcus aureus are better and the weaker and better antibacterial effects on staphylococcus epidermidis) when being matched, and the antibacterial efficacy is further improved by adding the nidus Collocaliae acid; from the results of examples 1 and 5, when the amount of dandelion extract in the raw materials is too large, the antibacterial effect is slightly weakened; from the results of examples 1 and 6, when the amount of the honeysuckle extract in the raw materials is excessive, the antibacterial effect is slightly weakened; from the results of examples 1 and 7, when the amount of the sophora flower extract in the raw materials is too large, the antibacterial effect is slightly weakened; from examples 1 and 8, it is apparent that the antibacterial effect is reduced when the amount of purslane extract in the raw material is too large. From example 1 and comparative examples 1 to 4, when the dandelion extract, the honeysuckle extract, the sophora flower extract or the purslane extract are absent from the raw materials, the antibacterial effect is obviously reduced; in the whole, the four raw materials are best in compatibility.

Test example 4

5 alpha reductase inhibition test

Precisely weighing 36mg testosterone, and fixing the volume to 25mL with absolute ethyl alcohol, wherein the concentration is 5mmol/L; NADPH solution: precisely weighing 42.5mg of NADPH tetrasodium salt, and fixing the volume to 25mL with water, wherein the concentration is 2mmol/L; enzyme extraction buffer solution: 0.32mol/L sucrose, 0.1mmol/L DTT,20mmol/L sodium phosphate, pH6.5. The reaction solution: 1mmol/L DTT,20mmo/L sodium phosphate.

The reaction components were sequentially (155. Mu.L of reaction solution, constant temperature at 37 ℃ C.; 10. Mu.L of testosterone; 10. Mu.L of NADPH; the compositions of the sample examples and comparative examples; 20. Mu.L of 5α -reductase) and the change of absorbance of the reaction system at 340nm within 4min was continuously monitored. According to the calculation formulas of the NADPH standard curve, the inhibitor activity unit and the inhibition percentage, the inhibition rate of the 5 alpha-reductase can be calculated.

Inhibitor activity unit definition: in a reaction system at 37 ℃, one enzyme activity unit is deactivated to one inhibitor activity unit. Percent inhibition = (inhibitor activity/enzyme activity) ×100%. The test results are shown in Table 6.

Test example 5

Tyrosinase inhibition rate assay

The compositions of examples and comparative examples were diluted to 0.2mg/mL in Phosphate Buffer (PBS) with pH=6.8 at 0.2mol/L as a solvent.

0.9mL of the solution prepared from the above sample and having a concentration of 0.2mg/mL was taken, 1mL of L-tyrosine (0.3 mol) and 1mL of Phosphate Buffer (PBS) having pH=6.8 were added, the mixture was preheated in a constant temperature water tank at 37℃for 10 minutes, 0.1mL of an aqueous tyrosinase solution (1.0 mg/mL, mcllvans buffer) was added thereto, the mixture was stirred well, and after a reaction at 37℃for 15 minutes, absorbance (D1) at 475nm was measured. The same reaction was performed using the phosphate buffer instead of the sample, and the absorbance (D2) was measured. For control, absorbance (D3) was measured without the addition of sample solution and tyrosinase. Tyrosinase activity inhibition was calculated using the following formula. Tyrosinase activity inhibition ratio (%) = [ (D2-D1)/(D2-D3) ]x100%. The test results are shown in Table 6.

TABLE 6

Sample of	5 alpha-reductase inhibition ratio (%)	Tyrosinase inhibition rate (%)
			Example 1	92.89	78.59
Example 2	89.69	61.87
			Example 3	60.32	53.23
Example 4	86.06	73.87
			Example 5	88.07	67.31
Example 6	81.51	66.22
			Example 7	91.42	55.26
Example 8	88.59	53.78
			Comparative example 1	72.75	62.02
Comparative example 2	76.98	47.72
			Comparative example 3	52.82	72.53
Comparative example 4	60.50	57.22
			Fusidic acid	68.84	-19.33

According to the table data, the dandelion extract, the honeysuckle extract, the sophora flower extract and the purslane extract obtained by the preparation method are matched for use, so that the effect of inhibiting 5 alpha reductase and tyrosinase is good, and the efficacy is further improved by adding the bird's nest acid; from examples 1 and 5, it is apparent that when the amount of dandelion extract in the raw material is too large, the effect of inhibiting 5a reductase and tyrosinase is reduced; as is clear from examples 1 and 6, when the amount of the honeysuckle extract in the raw material is too large, the effect of inhibiting 5a reductase and tyrosinase is reduced; from examples 1 and 7, it is apparent that when the amount of the extract of Sophora japonica flower is too large, the effect of inhibiting 5. Alpha. Reductase and tyrosinase is reduced; from examples 1 and 8, it is evident that when the amount of purslane extract used in the raw material is too large, the effect of inhibiting 5. Alpha. Reductase and tyrosinase is reduced; when any plant extract is absent from the composition, the inhibition effect on 5α reductase and tyrosinase is greatly reduced.

The applicant states that the process of the invention is illustrated by the above examples, but the invention is not limited to, i.e. does not mean that the invention must be carried out in dependence on the above process steps. It should be apparent to those skilled in the art that any modification of the present invention, equivalent substitution of selected raw materials, addition of auxiliary components, selection of specific modes, etc. fall within the scope of the present invention and the scope of disclosure.

Claims

1. A composition with a multi-way acne removing effect is characterized in that raw materials of the composition comprise dandelion extract, honeysuckle extract, sophora flower extract and purslane extract.

2. The composition with the multi-way acne-removing effect according to claim 1, wherein the raw materials of the composition comprise 1-2 parts by weight of dandelion extract, 1-2 parts by weight of honeysuckle extract, 1-2 parts by weight of sophora japonica extract and 1-2 parts by weight of purslane extract.

3. The composition with multi-path acne-removing efficacy according to claim 1 or 2, wherein the raw materials of the composition further comprise 0.05-0.15 parts of nidus Collocaliae acid.

4. A composition having multi-way acne-removing effect according to any one of claims 1 to 3, wherein the dandelion extract, the honeysuckle extract, the sophora flower extract and the purslane extract are extracted by an extraction method comprising a water extraction method and/or an alcohol extraction method.

5. The composition with multi-path acne-removing efficacy according to claim 4, wherein the aqueous extraction is performed by a method comprising: pulverizing plant, extracting with water, ultrasonic extracting, centrifuging, concentrating, and lyophilizing to obtain water extract of plant.

6. The composition with multi-path acne removing effect according to claim 5, wherein the ratio of the water extract to the liquid extract is 1 (18-22);

preferably, the temperature of the water extraction is 75-85 ℃ and the time is 0.5-1.5h;

preferably, the ultrasonic extraction time is 8-12min;

preferably, the rotational speed of the centrifugation is 3500-4500rpm, and the time is 3-8min;

preferably, the concentration is to 1/5 of the original volume.

7. The composition with multi-path acne-removing efficacy according to claim 4, wherein the alcohol extraction is performed by a method comprising: pulverizing the plant, extracting with alcohol, centrifuging, concentrating, and freeze drying to obtain the plant extract.

8. The composition with multi-path acne removing effect according to claim 7, wherein the alcohol extraction liquid ratio is 1 (12-18);

preferably, the extracting agent adopted by the alcohol extraction is ethanol water solution;

preferably, the mass percentage of the ethanol in the ethanol water solution is 60-70%;

preferably, the temperature of the alcohol extraction is 75-85 ℃ and the time is 1.5-2.5h;

preferably, the concentration is to 1/5 of the original volume.

9. The composition with multi-path acne-removing efficacy according to any one of claims 1 to 8, wherein the composition comprises dandelion alcohol extract, honeysuckle alcohol extract, sophora flower alcohol extract, purslane alcohol extract and nidus Collocaliae acid;

10. A method for preparing the composition with multi-path acne-removing efficacy according to any one of claims 1 to 9, wherein the method comprises: and uniformly mixing the dandelion extract, the honeysuckle extract, the sophora flower extract and the purslane extract to obtain the composition.