CN117471102A - Human bone metabolism marker Galanin double-colloidal gold detection test strip - Google Patents
Human bone metabolism marker Galanin double-colloidal gold detection test strip Download PDFInfo
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- 238000012360 testing method Methods 0.000 title claims abstract description 79
- 238000001514 detection method Methods 0.000 title claims abstract description 71
- 102000019432 Galanin Human genes 0.000 title claims abstract description 36
- 101800002068 Galanin Proteins 0.000 title claims abstract description 36
- SLZIZIJTGAYEKK-CIJSCKBQSA-N molport-023-220-247 Chemical compound C([C@@H](C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=1N=CNC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1N=CNC=1)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(N)=O)NC(=O)[C@H]1N(CCC1)C(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)CN)[C@@H](C)O)C1=CNC=N1 SLZIZIJTGAYEKK-CIJSCKBQSA-N 0.000 title claims abstract description 35
- 230000004097 bone metabolism Effects 0.000 title claims abstract description 25
- 239000003550 marker Substances 0.000 title claims abstract description 23
- 239000008280 blood Substances 0.000 claims abstract description 43
- 210000004369 blood Anatomy 0.000 claims abstract description 43
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims abstract description 34
- 238000001179 sorption measurement Methods 0.000 claims abstract description 26
- 239000002390 adhesive tape Substances 0.000 claims abstract description 25
- 239000010931 gold Substances 0.000 claims abstract description 20
- 229910052737 gold Inorganic materials 0.000 claims abstract description 20
- 238000000034 method Methods 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000012528 membrane Substances 0.000 claims abstract description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 39
- 210000001015 abdomen Anatomy 0.000 claims description 20
- 238000003908 quality control method Methods 0.000 claims description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- 238000010521 absorption reaction Methods 0.000 claims description 8
- 239000000126 substance Substances 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 6
- 238000011084 recovery Methods 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 229920000742 Cotton Polymers 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 241001052560 Thallis Species 0.000 claims description 3
- 239000011127 biaxially oriented polypropylene Substances 0.000 claims description 3
- 229920006378 biaxially oriented polypropylene Polymers 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 239000002699 waste material Substances 0.000 claims description 3
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 2
- 238000003912 environmental pollution Methods 0.000 claims description 2
- 239000003921 oil Substances 0.000 claims description 2
- 229910052710 silicon Inorganic materials 0.000 claims description 2
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- 230000017531 blood circulation Effects 0.000 abstract description 8
- 238000009792 diffusion process Methods 0.000 abstract description 2
- 210000000988 bone and bone Anatomy 0.000 description 8
- 208000001132 Osteoporosis Diseases 0.000 description 7
- 230000010072 bone remodeling Effects 0.000 description 3
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- 230000011164 ossification Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
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- 108091007433 antigens Proteins 0.000 description 1
- 210000001188 articular cartilage Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 229920002521 macromolecule Polymers 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000963 osteoblast Anatomy 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150015—Source of blood
- A61B5/150022—Source of blood for capillary blood or interstitial fluid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/151—Devices specially adapted for taking samples of capillary blood, e.g. by lancets, needles or blades
- A61B5/15101—Details
- A61B5/15103—Piercing procedure
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/157—Devices characterised by integrated means for measuring characteristics of blood
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
- G01N33/54387—Immunochromatographic test strips
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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- Health & Medical Sciences (AREA)
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- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Pathology (AREA)
- General Health & Medical Sciences (AREA)
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- Urology & Nephrology (AREA)
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- Surgery (AREA)
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- Analytical Chemistry (AREA)
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- Public Health (AREA)
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- Heart & Thoracic Surgery (AREA)
- Medical Informatics (AREA)
- Food Science & Technology (AREA)
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Abstract
The invention discloses a human bone metabolism marker Galanin double-colloidal gold detection test strip, which comprises the following components: the PVC bottom plate, the one end surface of PVC bottom plate is connected with the sample pad, the one end adhesion of sample pad has the gold mark pad, the one end that the gold mark pad was relative to the gold mark pad is provided with the NC membrane, the inside gomphosis of NC membrane has T line and C line, the other end of NC membrane is connected with the pad that absorbs water. According to the Galanin double-colloidal gold detection test paper and the detection device method for the bone metabolism marker, the release paper is separated from the bottom surface of the transparent adhesive tape by the aid of the hand-held pulling piece, then the transparent adhesive tape on two sides of the detection test paper is connected to the clean workbench, then collected 2-3 drops of blood are dripped to the sample adsorption port on the surface of the sample pad, then blood flows through the adsorption pad, then the blood flows to the gold mark pad, detection results are analyzed, the detection results can be detected more quickly by the aid of the adsorption pad, and the time required for detection is prevented from being increased due to blood diffusion.
Description
Technical Field
The invention relates to the technical field of medical science, in particular to a human bone metabolism marker Galanin double-colloidal gold detection test strip.
Background
Bone metabolic balance is an important link in the metabolic balance of the body, and bone formation/bone resorption coupling balance is the basis for normal bone remodeling. However, when bone resorption is greater than bone formation, high turnover rates of bone remodeling occur, which results in decreased bone mass and bone mass, leading to osteoporosis. In clinic, dual-energy X-rays are diagnostic gold standards of osteoporosis, and the results can be detected after the osteoporosis is caused, so that no obvious detection results exist in the period of beginning to reduce the bone mass and reducing the bone mass, the hysteresis is serious, the further deterioration of the condition is easily caused, and the social public service and the family burden of patients are increased;
galanin (Galanin) is widely distributed in human central and peripheral nervous tissues, and is distributed in gastrointestinal tract, connective tissue, articular cartilage and human bones. Modern science has shown that Galanin can act on bone metabolic process, promote osteoblast proliferation, inhibit osteoclast activity, and thereby improve bone remodeling disorders. By detecting the Galanin level in the blood sample, the bone metabolism state of the organism can be effectively reflected, and a positive method is provided for early screening, prevention and effective treatment of osteoporosis.
There is a need for technology upgrades to address the above issues to meet market demands.
Disclosure of Invention
The invention aims to provide a human bone metabolism marker Galanin double-colloidal gold detection test strip, which aims to solve the problems that in the background art, double-energy X-rays are clinical diagnosis gold standards of osteoporosis, and the detection result can be usually detected after the onset of the osteoporosis, and no obvious detection result exists in the period of beginning to reduce bone mass and reducing bone mass, the hysteresis is serious, the further deterioration of illness state is easily caused, and the social public service and the household burden of patients are increased.
In order to achieve the above purpose, the present invention provides the following technical solutions: a human bone metabolism marker Galanin double-colloidal gold test strip, comprising: the sample pad is connected to one end surface of the PVC bottom plate, a gold mark pad is adhered to one end of the sample pad, an NC film is arranged at one end of the gold mark pad opposite to the gold mark pad, a T line and a C line are embedded in the NC film, and a water absorption pad is connected to the other end of the NC film;
the PVC bottom plate is characterized in that a transparent adhesive tape is connected to the outer side wall of the PVC bottom plate, release paper is attached to the bottom surface of the transparent adhesive tape, a poking piece is connected to one end of the release paper, a sample adsorption port is formed in the surface of the sample pad, and an adsorption pad is embedded in the sample pad close to the sample adsorption port.
Preferably, the sample pad, the gold-labeled pad, the NC film, the T line, the C line and the water absorbing pad are all positioned on the surface of the PVC bottom plate.
Preferably, the transparent adhesive tape is BOPP membrane material, transparent adhesive tape is provided with four, and every two transparent adhesive tapes are about the longitudinal axis symmetry of PVC bottom plate.
Preferably, the release paper and the poking piece are both made of paper coated with anti-sticking substances, and a silicon oil layer of the release paper is contacted with a primer layer of the transparent adhesive tape.
Preferably, the adsorption pad is embedded in the sample pad in a honeycomb shape and is connected with the gold mark pad, and the adsorption pad is made of sponge materials.
Preferably, the method comprises the following steps:
s1, selecting alcohol cotton: selecting alcohol sponge with proper size in the alcohol box, and clamping the alcohol sponge by using medical forceps;
s2, fingertip disinfection: selecting the abdomen of the finger tip of the person to be detected, and wiping and sterilizing by using alcohol sponge;
s3, puncturing skin: then pricking the sterilized abdomen of the finger tip of the person to be detected by using the disposable blood taking needle;
s4, blood sample collection: collecting 2-3 drops of blood flowing out of the abdomen of the finger tip;
s5, detecting a blood sample: dropping 2-3 drops of blood into a sample area of the test paper, and then horizontally placing the test paper on a clean tabletop;
s6, secondary fingertip disinfection: the medical forceps are clamped with the alcohol sponge again to wipe and disinfect the abdomen of the punctured finger tip;
s7, comparing results: performing contrast analysis on the detection test paper which is placed still for 10 min;
s8, color line display: the collected blood sample can flow to a detection line and a quality control line inside the detection test paper, and the surface of the detection test paper can display a color line;
s9, obtaining a result template: comparing a color line displayed on the surface of the detection test paper with a sample plate, developing color by a quality control line, and not developing color by a detection line, wherein the Galanin level in the sample is lower than a detection limit, namely negative, the quality control line and the detection line are both developed, the Galanin level in the sample is equal to or higher than the detection limit, namely positive, the quality control line is not developed, and the operation is incorrect or the test paper strip is failed;
s10, data recording: after the color line comparison of the test paper is detected, the comparison data is transcribed by a detector, and all-weather data recording is carried out by a camera;
s11, waste recovery: and (3) carrying out centralized recovery treatment on the detected detection test paper, the wiped alcohol sponge and the disposable blood taking needle, and finishing the double-colloidal gold detection.
Preferably, the alcohol content of the alcohol sponge in the S1 is 70% -75%, so that the alcohol sponge can penetrate through the inner part of the abdomen thalli of the finger tip, and the whole protein of the bacteria is coagulated and denatured, so that the aim of sterilization is fulfilled.
Preferably, in the step S2, the disposable blood taking needle is rubbed back and forth on the abdomen of the finger tip of the person to be detected for 3-5 times by using an alcohol sponge, and the specification of the disposable blood taking needle in the step S3 is 0.55 x 19mm.
Preferably, the recording code rate of the camera used in the step S7 is 25Mbps, and the resulting template in the step 7 is color A4 paper.
Preferably, in the step S11, the detection test paper, the alcohol sponge and the disposable blood taking needle are collected in a concentrated mode, and the purpose of environmental pollution caused by random discarding of chemical products is avoided.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the Galanin double-colloidal gold detection test paper and the detection device method for the bone metabolism marker, the release paper is separated from the bottom surface of the transparent adhesive tape by the aid of the hand-held pulling piece, then the transparent adhesive tape on two sides of the detection test paper is connected to the clean workbench, then collected 2-3 drops of blood are dripped to the sample adsorption port on the surface of the sample pad, then blood flows through the adsorption pad, then the blood flows to the gold mark pad, then the blood is influenced by the water absorption pad, so that the blood flows to a T line and a C line, then the detection result is displayed through the color line, the detection result is analyzed, the detection result can be detected more quickly by using the adsorption pad, and the detection time is increased due to blood diffusion, so that the detection test paper and the detection device can be effectively and quickly applied to early screening and preventive workers of osteoporosis in a vast population of a base layer.
2. According to the bone metabolism marker Galanin double-colloidal gold detection test paper and the detection device method, the abdomen of a finger of a person to be detected is wiped and disinfected by using an alcohol cotton ball, then the skin is punctured by using a disposable blood taking needle, 2-3 drops of blood are close to a blood receiving area of the test paper, the finger tips are wiped and disinfected again, then the detection test paper is horizontally placed on a clean workbench for waiting for 10min, then the color development condition of the surface of the detection test paper is compared with a result on a sample plate, then the person to be detected records data, and meanwhile, the camera records the data in real time, so that the data loss is avoided, and finally, the used detection test paper, the alcohol cotton and the disposable blood taking needle are collected in a concentrated mode, so that chemical supplies are prevented from being discarded at will, and the environment is polluted.
Drawings
FIG. 1 is a schematic perspective view of a test strip according to the present invention;
FIG. 2 is a schematic diagram of a cross-sectional structure of a test strip according to the present invention;
FIG. 3 is a schematic view of a release paper connection structure according to the present invention;
FIG. 4 is a schematic cross-sectional view of an absorbent pad according to the present invention;
FIG. 5 is a schematic diagram of the steps of the present invention;
FIG. 6 is a schematic view of the portion of FIG. 5 according to the present invention.
In the figure: 1. a PVC bottom plate; 2. a sample pad; 3. a gold mark pad; 4. NC film; 5. a T line; 6. a C line; 7. a water absorbing pad; 8. a transparent adhesive tape; 9. release paper; 10. a pulling piece; 11. a sample adsorption port; 12. and (5) an adsorption pad.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-6, the present invention provides a technical solution: a human bone metabolism marker Galanin double-colloidal gold test strip, comprising: the device comprises a PVC bottom plate 1, wherein a sample pad 2 is connected to one end surface of the PVC bottom plate 1, a gold mark pad 3 is adhered to one end of the sample pad 2, an NC film 4 is arranged at one end of the gold mark pad 3 opposite to the gold mark pad 3, a T line 5 and a C line 6 are embedded in the NC film 4, and a water absorption pad 7 is connected to the other end of the NC film 4;
the outer side wall of PVC bottom plate 1 is connected with transparent adhesive tape 8, the bottom surface of transparent adhesive tape 8 attaches and has from type paper 9, from the one end of type paper 9 to be connected with plectrum 10, sample adsorption port 11 has been seted up to sample pad 2's surface, sample pad 2 is close to the inside gomphosis of sample adsorption port 11 and has adsorption pad 12.
Referring to fig. 1-2, the sample pad 2, the gold mark pad 3, the NC film 4, the T line 5, the C line 6, and the water absorbing pad 7 are all positioned on the surface of the PVC base plate 1;
referring to fig. 1-3, the transparent adhesive tapes 8 are made of BOPP film materials, and four transparent adhesive tapes 8 are provided, and every two transparent adhesive tapes 8 are symmetrical about the longitudinal central axis of the PVC base plate 1;
referring to fig. 3, the release paper 9 and the pulling sheet 10 are both made of paper coated with anti-sticking substances, and the silicone oil layer of the release paper 9 is contacted with the primer layer of the transparent adhesive tape 8;
referring to fig. 2, the adsorption pad 12 is embedded in the sample pad 2 in a honeycomb shape and is connected with the gold mark pad 3, and the adsorption pad 12 is made of a sponge material;
referring to fig. 5-6, the steps include:
s1, selecting alcohol cotton: selecting alcohol sponge with proper size in the alcohol box, and clamping the alcohol sponge by using medical forceps;
s2, fingertip disinfection: selecting the abdomen of the finger tip of the person to be detected, and wiping and sterilizing by using alcohol sponge;
s3, puncturing skin: then pricking the sterilized abdomen of the finger tip of the person to be detected by using the disposable blood taking needle;
s4, blood sample collection: collecting 2-3 drops of blood flowing out of the abdomen of the finger tip;
s5, detecting a blood sample: dropping 2-3 drops of blood into a sample area of the test paper, and then horizontally placing the test paper on a clean tabletop;
s6, secondary fingertip disinfection: the medical forceps are clamped with the alcohol sponge again to wipe and disinfect the abdomen of the punctured finger tip;
s7, comparing results: performing contrast analysis on the detection test paper which is placed still for 10 min;
s8, color line display: the collected blood sample can flow to a detection line and a quality control line inside the detection test paper, and the surface of the detection test paper can display a color line;
s9, obtaining a result template: comparing a color line displayed on the surface of the detection test paper with a sample plate, developing color by a quality control line, and not developing color by a detection line, wherein the Galanin level in the sample is lower than a detection limit, namely negative, the quality control line and the detection line are both developed, the Galanin level in the sample is equal to or higher than the detection limit, namely positive, the quality control line is not developed, and the operation is incorrect or the test paper strip is failed;
s10, data recording: after the color line comparison of the test paper is detected, the comparison data is transcribed by a detector, and all-weather data recording is carried out by a camera;
s11, waste recovery: and (3) carrying out centralized recovery treatment on the detected detection test paper, the wiped alcohol sponge and the disposable blood taking needle, and finishing the double-colloidal gold detection.
Referring to fig. 5-6, the alcohol content of the alcohol sponge in the step S1 is 70% -75%, so that the alcohol sponge can penetrate through the interior of the abdomen thalli of the finger tip, and the whole protein of the bacteria is coagulated and denatured, so that the aim of sterilization is fulfilled;
referring to fig. 5, in S2, wiping back and forth on the abdomen of the finger tip of the person to be detected with an alcohol sponge for 3-5 times, wherein the specification of the disposable blood taking needle in S3 is 0.55 x 19mm;
referring to fig. 5-6, the recording code rate of the camera adopted in the step S7 is 25Mbps, and the resulting template in the step 7 adopts color A4 paper;
referring to fig. 5, in S11, the test paper, the alcohol sponge and the disposable blood taking needle are collected in a concentrated manner, so as to avoid the pollution of the environment caused by random discarding of chemical products.
Working principle: as shown in fig. 1-6, when the bone metabolism marker Galanin double-colloidal gold test paper and the detection device method are used, the bone metabolism marker Galanin double-colloidal gold test paper and the detection device are used for detecting Galanin protein, and colloidal gold is a colloidal gold-labeled biological macromolecule which can be used as a chromogenic medium to be combined with an antibody in the process of detecting the bone metabolism marker Galanin. The gold-labeled antibody, namely the colloidal gold-labeled antibody, can recognize the Galanin antigen to form a 'viral antigen-gold-labeled antibody' complex, then continuously flows towards the direction of a water absorption pad at the tail end of the test paper, is captured by the T-line antibody specific monoclonal Galanin antibody on a detection line, and forms a 'colloidal gold antibody-Galanin antigen-T-line 5 antibody' complex on a T-line 5. Since colloidal gold is accumulated in a large amount, the T line 5 appears red. Because the amount of the gold-labeled antibody in the test paper is excessive, the gold-labeled antibody is not totally trapped in the T line 5, and can still flow in the direction of the water absorption pad. When the gold-labeled antibody is detected to the C line 6, the C line 6 antibody is captured, the gold-labeled antibody-C line 6 antibody compound is formed on the C line 6, therefore, the C line 6 also shows red color, the release paper 9 is separated on the bottom surface of the transparent adhesive tape 8 through the handheld plectrum 10, then the transparent adhesive tape 8 on two sides of the detection test paper is connected to a clean workbench, 2-3 drops of blood are collected to the sample adsorption port 11 on the surface of the sample pad 2, then blood flows through the adsorption pad 12, then blood flows to the gold-labeled pad 3, then the blood is influenced by the water absorption pad 7, so that the blood flows to the T line 5 and the C line 6, then the detection result is displayed through a color line, the detection result is analyzed, the use of the adsorption pad 12 can be faster, the detection result is prevented from being diffused due to blood, the increase of detection time, the abdomen of a person to be detected is sterilized through an alcohol cotton ball, then the skin is pierced through a disposable needle, then 2-3 drops of blood are next to a blood receiving area, the collected, the finger tip is again wiped, the finger tip is placed on the test paper again, the test paper is placed on the test paper to the surface of the test paper to be detected, the test paper is placed on the test paper, the surface of the test paper is kept clean, and the test paper is used up, the test paper is free from being used up, and the condition of a test paper is free from being compared with a test paper is free, and the condition of a test paper is read by a test paper is free from the test paper, and the test paper is used for detecting the test paper is free, and the test paper is used for the test paper, and the test paper is free from the test paper and has the quality data is free to be used for and read.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. A bone metabolism marker Galanin double-colloidal gold test paper, comprising: PVC bottom plate (1), its characterized in that: one end surface of the PVC bottom plate (1) is connected with a sample pad (2), one end of the sample pad (2) is adhered with a gold mark pad (3), one end of the gold mark pad (3) opposite to the gold mark pad (3) is provided with an NC film (4), a T line (5) and a C line (6) are embedded in the NC film (4), and the other end of the NC film (4) is connected with a water absorption pad (7);
the PVC bottom plate (1) lateral wall is connected with transparent adhesive tape (8), the bottom surface of transparent adhesive tape (8) attaches and has from type paper (9), the one end from type paper (9) is connected with plectrum (10), sample adsorption port (11) have been seted up on the surface of sample pad (2), sample pad (2) are close to the inside gomphosis of sample adsorption port (11) and have adsorption pad (12).
2. The bone metabolism marker Galanin double-colloidal gold test paper according to claim 1, wherein: the sample pad (2), the gold mark pad (3), the NC film (4), the T line (5), the C line (6) and the water absorption pad (7) are all positioned on the surface of the PVC bottom plate (1).
3. The bone metabolism marker Galanin double-colloidal gold test paper according to claim 1, wherein: transparent adhesive tape (8) are BOPP membrane material, transparent adhesive tape (8) are provided with four, and every two transparent adhesive tape (8) are with the longitudinal axis symmetry of PVC bottom plate (1).
4. The bone metabolism marker Galanin double-colloidal gold test paper according to claim 1, wherein: the release paper (9) and the poking piece (10) are both made of paper coated with anti-sticking substances, and a silicon oil layer of the release paper (9) is contacted with a primer layer of the transparent adhesive tape (8).
5. The bone metabolism marker Galanin double-colloidal gold test paper according to claim 1, wherein: the adsorption pad (12) is embedded in the sample pad (2) in a honeycomb shape and is connected with the gold mark pad (3), and the adsorption pad (12) is made of sponge materials.
6. The method for detecting the bone metabolism marker Galanin double-colloidal gold is characterized by comprising the following steps of:
s1, selecting alcohol cotton: selecting alcohol sponge with proper size in the alcohol box, and clamping the alcohol sponge by using medical forceps;
s2, fingertip disinfection: selecting the abdomen of the finger tip of the person to be detected, and wiping and sterilizing by using alcohol sponge;
s3, puncturing skin: then pricking the sterilized abdomen of the finger tip of the person to be detected by using the disposable blood taking needle;
s4, blood sample collection: collecting 2-3 drops of blood flowing out of the abdomen of the finger tip;
s5, detecting a blood sample: dropping 2-3 drops of blood into a sample area of the test paper, and then horizontally placing the test paper on a clean tabletop;
s6, secondary fingertip disinfection: the medical forceps are clamped with the alcohol sponge again to wipe and disinfect the abdomen of the punctured finger tip;
s7, comparing results: performing contrast analysis on the detection test paper which is placed still for 10 min;
s8, color line display: the collected blood sample can flow to a detection line and a quality control line inside the detection test paper, and the surface of the detection test paper can display a color line;
s9, obtaining a result template: comparing a color line displayed on the surface of the detection test paper with a sample plate, developing color by a quality control line, and not developing color by a detection line, wherein the Galanin level in the sample is lower than a detection limit, namely negative, the quality control line and the detection line are both developed, the Galanin level in the sample is equal to or higher than the detection limit, namely positive, the quality control line is not developed, and the operation is incorrect or the test paper strip is failed;
s10, data recording: after the color line comparison of the test paper is detected, the comparison data is transcribed by a detector, and all-weather data recording is carried out by a camera;
s11, waste recovery: and (3) carrying out centralized recovery treatment on the detected detection test paper, the wiped alcohol sponge and the disposable blood taking needle, and finishing the double-colloidal gold detection.
7. The method for detecting the bone metabolism marker Galanin double-colloidal gold according to claim 6, wherein the method comprises the following steps: the alcohol content of the alcohol sponge in the S1 is 70% -75%, so that the alcohol sponge can penetrate through the inner part of the abdomen thalli of the finger tip, and the whole protein of the bacteria is coagulated and denatured, so that the aim of sterilization is fulfilled.
8. The method for detecting the bone metabolism marker Galanin double-colloidal gold according to claim 6, wherein the method comprises the following steps: in the step S2, the abdomen of the finger tip of the person to be detected is rubbed back and forth for 3-5 times by using the alcohol sponge, and the specification of the disposable blood taking needle in the step S3 is 0.55 x 19mm.
9. The method for detecting the bone metabolism marker Galanin double-colloidal gold according to claim 6, wherein the method comprises the following steps: the recording code rate of the camera adopted in the step S7 is 25Mbps, and the color A4 paper is adopted as the result template in the step 7.
10. The method for detecting the bone metabolism marker Galanin double-colloidal gold according to claim 6, wherein the method comprises the following steps: and S11, the detection test paper, the alcohol sponge and the disposable blood taking needle are collected in a concentrated mode, and the purpose of environmental pollution caused by random discarding of chemical products is avoided.
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