CN117448162A - Microscope is with multi-functional tissue environment culture apparatus - Google Patents

Microscope is with multi-functional tissue environment culture apparatus Download PDF

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Publication number
CN117448162A
CN117448162A CN202311509776.1A CN202311509776A CN117448162A CN 117448162 A CN117448162 A CN 117448162A CN 202311509776 A CN202311509776 A CN 202311509776A CN 117448162 A CN117448162 A CN 117448162A
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CN
China
Prior art keywords
sealing
end cover
slide
multifunctional
microscope
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Pending
Application number
CN202311509776.1A
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Chinese (zh)
Inventor
杨懿
陈宇翔
严峻
李增
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Zhejiang Hehu Technology Co ltd
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Zhejiang Hehu Technology Co ltd
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Priority to CN202311509776.1A priority Critical patent/CN117448162A/en
Publication of CN117448162A publication Critical patent/CN117448162A/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/46—Means for fastening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a multifunctional tissue environment culture device for a microscope, which comprises a multifunctional sealing end cover and a culture dish, wherein the culture dish comprises a bottom matrix, a replaceable slide and a slide fixing device; a slide fixing device is arranged in the middle of the bottom matrix, and a replaceable slide is fixed on the slide fixing device; the multifunctional sealing end cover is arranged above the culture dish, a through hole is formed in the middle of the top surface of the multifunctional sealing end cover, an acrylic transparent plate is arranged in the through hole, a PI heating film is arranged at the bottom of the through hole, and a power wire of the PI heating film penetrates out of the side wall of the multifunctional sealing end cover; the side wall of the multifunctional sealing end cover is provided with a pressure transmitter, a pressure regulating device, an air pipe joint and a temperature and humidity sensor; the air pipe connector is connected with an ultrasonic heater; the pressure transmitter and the temperature and humidity sensor are connected with a paperless recorder; solves the problem that the carbon dioxide culture system for the microscope at home and abroad can not simulate the pressure environment.

Description

Microscope is with multi-functional tissue environment culture apparatus
Technical Field
The invention relates to the technical field of tissue culture, in particular to a multifunctional tissue environment culture device for a microscope.
Background
The research of organoids, cancer cells, living cells and tissue environments belongs to the leading edge research in the medical field.
In a paper in journal of Cell Stem Cell, researchers have shown that physical pressure squeezes cells, squeezing the Cell contents, can trigger organoids to grow and divide faster than normal.
In the paper of the 'in vitro simulation of the influence of the carbon dioxide pneumoperitoneum environment on the proliferation and apoptosis of cervical cancer cells' and the 'influence of the carbon dioxide pneumoperitoneum environment on the proliferation and apoptosis of ovarian cancer cells', the conclusion is that: the in vitro simulation of the CO2 environment has obvious inhibition effect on the growth of cancer cells, the inhibition degree of the in vitro simulation of the CO2 environment is obviously related to the pressure and the action time of CO2 gas, and the pressure and the action time of CO2 are related to the apoptosis process of cervical cancer cells.
In the paper of the continuous pressure culture environment on proliferation of rabbit bone marrow stromal cells, the conclusion is that: the continuous pressure culture environment can inhibit proliferation of rabbit bone marrow stromal stem cells.
The existing living cell culture systems for domestic and foreign microscopes are not provided with a gas pressure loading device, and the pressure surrounding environment cannot be formed by changing the pressure to stimulate the cells, so that the reaction of the cells in the pressure state is researched; when there is a wound on the skin surface, blood flows out because the pressure in the human body is greater than the atmospheric pressure, and as such, cells in the human body are under the action of the pressure, and when the cells leave the human body, the original pressure disappears, and the pressure-sensitive factors in the cells are included, so that the expression of certain proteins of the cells is influenced. The pressure environment in the body can be simulated, the pressure surrounding environment is formed by changing the stimulation of the pressure to the cells, and the reaction of the cells under the pressure state is studied.
In the above examples, the number of living cells placed in a special carbon dioxide pressure tank was measured mainly by flow cytometry PI staining to analyze the cell cycle and MTT colorimetry, and the observation under a microscope was not possible.
The existing domestic and foreign equipment cannot observe the real-time state of cells in a pressure environment and detailed cell inhibition time in real time through a microscope.
Disclosure of Invention
The invention aims to provide a multifunctional tissue environment culture device for a microscope, which aims to overcome the defects in the prior art.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the application discloses a microscope is with multi-functional tissue environment culture apparatus, including multi-functional sealed end cover and culture dish, the culture dish includes bottom base member, interchangeable slide and slide fixing device; a slide fixing device is arranged in the middle of the bottom matrix, and a replaceable slide is fixed on the slide fixing device; the multifunctional sealing end cover is arranged above the culture dish, a through hole is formed in the middle of the top surface of the multifunctional sealing end cover, an acrylic transparent plate is arranged in the through hole, a PI heating film is arranged at the bottom of the through hole and is positioned right above the replaceable glass slide, and a power line of the PI heating film penetrates out of the side wall of the multifunctional sealing end cover; the side wall of the multifunctional sealing end cover is provided with a pressure transmitter, a pressure regulating device, an air pipe joint and a temperature and humidity sensor; the air pipe connector is connected with an ultrasonic heater; the pressure transmitter and the temperature and humidity sensor are connected with a paperless recorder.
Preferably, the slide fixing device comprises a fixed iron sheet, a fixed magnet and a sealing ring, wherein a slide mounting hole is formed in the middle of the bottom substrate, the fixed iron sheet is arranged below the slide mounting hole, the fixed magnet is arranged above the slide mounting hole, the sealing ring is arranged between the fixed magnet and the fixed iron sheet, and the replaceable slide is positioned in the slide mounting hole.
Preferably, the bottom of the bottom substrate is embedded with a plurality of objective table magnets.
Preferably, the bottom substrate is provided with a plurality of end cover magnets, the bottom of the multifunctional sealing end cover is provided with a plurality of culture dish magnets, and the end cover magnets and the culture dish magnets are in one-to-one correspondence and have opposite magnetic poles; the multifunctional sealing end cover is fastened with the culture dish through a plurality of locking screws, and a compressed silica gel sealing ring is arranged between the multifunctional sealing end cover and the culture dish.
Preferably, the pressure regulating device comprises a pressure regulating valve body, a sealing block, a spring and a pressure regulating nut, wherein a cavity is arranged in the pressure regulating valve body, one end of the cavity is provided with a connecting pipeline, the other end of the connecting pipeline is communicated with the inside of the culture device, the other end of the connecting pipeline is communicated with the external environment, one end, close to the culture device, in the cavity is provided with the sealing block, the outer diameter of the sealing block is larger than the inner diameter of the connecting pipeline, one end, close to the external environment, in the cavity is provided with the pressure regulating nut, and the pressure regulating nut is connected with the sealing block through the spring.
Preferably, a guide adapter is arranged between the spring and the sealing block, one side of the guide adapter is provided with a sealing block mounting groove, and the other side of the guide adapter is provided with a spring mounting groove; one end of the sealing block is fixedly arranged in the sealing block mounting groove, one end of the spring is fixedly arranged in the spring mounting groove, and the outer diameter of the guide adapter is matched with the inner diameter of the cavity.
Preferably, the pressure regulating nut is connected with the inner wall of the cavity through threads.
Preferably, a power wire sealing nut for sealing the PI heating film power wire is arranged on the side face of the multifunctional sealing end cover, the power wire sealing nut is connected with the side face of the multifunctional sealing end cover through threads, and a special-shaped sealing ring is arranged between the power wire sealing nut and the multifunctional sealing end cover.
Preferably, a clamping ring is arranged at the lower part of the acrylic transparent plate, the clamping ring is fixedly connected with the through hole through threads, and a gasket is arranged between the acrylic transparent plate and the clamping ring.
The invention has the beneficial effects that:
1. the split type design, the bottom base member design is similar to a can used repeatedly's culture dish, for the disposable plastic culture dish of current stage commonly used, only need change the cell climbing piece in the structure just can, not only be applicable to the cell climbing piece simultaneously, also can change marking cover glass, plastic sheet, cell counting board, slide. Compared with the price of a disposable plastic culture dish, the cost of long-term use is saved, and the application range is enlarged;
2. the control system is provided with the paperless recorder, the client does not need to look at the data in real time, the paperless recorder can record the specific temperature, humidity and pressure data of each time point in real time, and the client can directly extract the environmental information of the required time period;
3. the magnetic attraction type design can be directly adsorbed on a microscope objective table without installing screws, so that customer experience is improved;
the features and advantages of the present invention will be described in detail by way of example with reference to the accompanying drawings.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a multifunctional tissue environment culture device for a microscope according to the present invention;
FIG. 2 is an exploded view of the structure of the culture dish according to the present invention;
FIG. 3 is an exploded view of the construction of the multi-functional seal end cap of the present invention;
FIG. 4 is a schematic diagram of the pressure regulating device of the present invention;
FIG. 5 is a schematic view showing the structure of the culture system of the present invention;
the multifunctional sealing end cover comprises a 1-multifunctional sealing end cover, a 11-acrylic transparent plate, a 111-clamping ring, a 112-gasket, a 12-PI heating film, a 121-power line sealing nut, a 122-anisotropic sealing ring, a 2-culture dish, a 21-bottom substrate, a 211-slide mounting hole, a 212-objective table magnet, a 213-end cover magnet, a 22-replaceable slide, a 23-slide fixing device, a 231-fixed iron sheet, a 232-fixed magnet, a 233-sealing ring, a 234-compressed silica gel sealing ring, a 3-pressure transmitter, a 4-pressure regulating device, a 41-pressure regulating valve body, a 411-cavity, a 412-connecting pipeline, a 42-sealing block, a 43-spring, a 44-pressure regulating nut, a 45-guiding adapter, a 5-tracheal joint and a 6-temperature and humidity sensor.
Detailed Description
The present invention will be further described in detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the detailed description and specific examples, while indicating the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention. In addition, in the following description, descriptions of well-known structures and techniques are omitted so as not to unnecessarily obscure the present invention.
Referring to fig. 1, the multifunctional tissue environment culture device for a microscope comprises a multifunctional sealing end cover 1 and a culture dish 2, wherein the culture dish 2 comprises a bottom base 21, a replaceable slide 22 and a slide fixing device 23; a slide fixing device 23 is arranged in the middle of the bottom matrix 21, and a replaceable slide 22 is fixed on the slide fixing device 23; a multifunctional sealing end cover 1 is arranged above the culture dish 2, a through hole is formed in the middle of the top surface of the multifunctional sealing end cover 1, an acrylic transparent plate 11 is arranged in the through hole, a PI heating film 12 is arranged at the bottom of the through hole, the PI heating film 12 is positioned right above the replaceable slide 22, and a power line of the PI heating film 12 penetrates out of the side wall of the multifunctional sealing end cover 1; the side wall of the multifunctional sealing end cover 1 is provided with a pressure transmitter 3, a pressure regulating device 4, an air pipe joint 5 and a temperature and humidity sensor 6; the air pipe joint 5 is connected with an ultrasonic heater; the pressure transmitter 3 and the temperature and humidity sensor 6 are connected with a paperless recorder.
Wherein the air pipe joint 5 is connected with the sealed ultrasonic humidifier through an air pipe, as shown in figure 5, 100% CO in the air bottle 2 The air in the gas and the air pump passes through the gas proportional valve, and the gas proportional valve controls the gas concentration by controlling the flow rate of the gas through the gas proportional valve controller, for example: 20% CO is required 2 CO can be controlled by a gas proportional valve 2 The flow rate of (2) and the flow rate of air were 1L/min and 4L/min, respectively. The mixed gas with controlled concentration is connected with an ultrasonic humidifier, the high-frequency vibration of the atomizing sheet causes water in the humidifier to be thrown away from the water surface to generate elegant water mist, and the mixed gas takes away the water mist in the humidifier to ensure that the mixed gas is added with the first gasAnd flows into the culture system to ensure that the humidity inside the culture system is maintained at more than 95%.
The pressure transmitter 3 and the temperature and humidity sensor 6 are connected with a paperless recorder, the paperless recorder displays the temperature, humidity and pressure data in the culture system, and records the specific temperature, humidity and pressure data at each time point in real time, so that a customer can directly extract the environmental information of the required time period according to the needs.
Referring to fig. 2, the slide fixing device 23 includes a fixing iron plate 231, a fixing magnet 232 and a sealing ring 233, a slide mounting hole 211 is provided in the middle of the bottom substrate 21, the fixing iron plate 231 is provided below the slide mounting hole 211, the fixing magnet 232 is provided above the slide mounting hole 211, the sealing ring 233 is provided between the fixing magnet 232 and the fixing iron plate 231, and the replaceable slide 22 is located in the slide mounting hole 211.
The bottom of the bottom base 21 is embedded with a plurality of stage magnets 212.
The bottom substrate 21 is provided with a plurality of end cover magnets 213, the bottom of the multifunctional sealing end cover 1 is provided with a plurality of culture dish magnets, and the end cover magnets 213 and the culture dish magnets are in one-to-one correspondence and have opposite magnetic poles; the multifunctional sealing end cover 1 is fastened with the culture dish 2 through a plurality of locking screws, and a compressed silica gel sealing ring 234 is arranged between the multifunctional sealing end cover 1 and the culture dish 2.
A reusable culture dish 2, a fixed iron sheet 231 (circular hollow iron sheet) is adhered below a bottom substrate 21, and mainly for fixing a magnet 232 to provide a magnetic attraction force, a replaceable slide 22 is arranged on the bottom substrate 21, and the slide can be replaced by a cell climbing sheet, a streaking cover glass, a plastic sheet, a cell counting plate and a glass slide according to application scenes; the replaceable slide 22 is provided with a sealing ring 233 (silica gel gasket), which is mainly matched with the fixed magnet 232 to play a role in sealing and preventing the culture fluid on the replaceable slide 22 from flowing out; 6 objective table magnets 212 are adhered to the bottom base 21, so that the magnetic attraction of the magnetic attraction magnet to the microscope objective table can be realized directly, screws are not needed, and the operation time is saved. The bottom base 21 is adhered with 4 end cover magnets 213 and compressed silica gel sealing rings 234 simultaneously, so that magnetic attraction can be formed between the four end cover magnets and the four culture dish magnets on the multifunctional sealing end cover 1, and a sealing environment is formed by matching with the compressed silica gel sealing rings 234.
Referring to fig. 4, the pressure regulating device 4 includes a pressure regulating valve body 41, a sealing block 42, a spring 43 and a pressure regulating nut 44, a cavity 411 is disposed in the pressure regulating valve body 41, one end of the cavity 411 is provided with a connecting pipe 412, the other end of the connecting pipe 412 is communicated with the inside of the culture device, the other end of the connecting pipe is communicated with the external environment, one end, close to the culture device, in the cavity 411 is provided with the sealing block 42, the outer diameter of the sealing block 42 is greater than the inner diameter of the connecting pipe 412, one end, close to the external environment, in the cavity 411 is provided with the pressure regulating nut 44, and the pressure regulating nut 44 is connected with the sealing block 42 through the spring 43.
A guide adapter 45 is arranged between the spring 43 and the sealing block 42, one side of the guide adapter 45 is provided with a sealing block mounting groove, and the other side is provided with a spring mounting groove; one end of the sealing block 42 is fixedly mounted in the sealing block mounting groove, one end of the spring 43 is fixedly mounted in the spring mounting groove, and the outer diameter of the guiding adaptor 45 is matched with the inner diameter of the cavity 411.
The pressure regulating nut 44 is connected with the inner wall of the cavity 411 through threads.
The pressure regulating device 4 is characterized in that a plastic sealing block 42 is arranged in the pressure regulating valve body 41 to play a sealing role, and a guiding adapter 45 is connected to the plastic sealing block 42; the guide adapter 45 is connected with the spring 43 and plays a role in guiding and transmitting the spring force; the pressure regulating nut 44 and the pressure regulating valve body 41 are connected through threads, and clockwise rotation of the nut can provide downward pressure, and counterclockwise rotation is opposite. According to the pressure regulating principle, gas flows into the sealed cavity 411, so that certain pressure is formed in the cavity 411, the pressure is smaller than or equal to the pressure of the gas in the gas cylinder, the pressure of the gas in the pressure regulating device is larger than or equal to the spring force, the gas valve is opened, the gas flows out, and the pressure in the cavity is reduced, so that the pressure reaches the effect of regulating the pressure.
Referring to fig. 3, a power line sealing nut 121 for sealing the power line of the PI heating film 12 is disposed on the side surface of the multifunctional sealing end cover 1, the power line sealing nut 121 is connected with the side surface of the multifunctional sealing end cover 1 through threads, and a special-shaped sealing ring 122 is disposed between the power line sealing nut 121 and the multifunctional sealing end cover 1.
A clamping ring 111 is arranged at the lower part of the acrylic transparent plate 11, the clamping ring 111 is fixedly connected with the through hole through threads, and a gasket 112 is arranged between the acrylic transparent plate 11 and the clamping ring 111;
the power line sealing nut 121 and the plastic special-shaped sealing ring 122 are mainly used for sealing the power line coming out of the PI heating film 12, and because the power line sealing nut 121 and the plastic special-shaped sealing ring 122 are used for sealing the power line coming out of the PI heating film 12 in a pressure environment, the common traditional sealing mode has the possibility of failure, the novel power line sealing mode is adopted, the power line sealing nut 121 and the top end cover are connected through threads, the plastic special-shaped sealing ring 122 is a silica gel sealing ring with a special structure, an oblique angle groove is formed in the plastic special-shaped sealing ring, and the groove on the plastic special-shaped sealing ring 122 is extruded through rotating the power line sealing nut 121, so that the plastic special-shaped sealing ring and the power line form a tight sealing environment. The acrylic transparent plate 11 is arranged on the top end cover, and is in threaded connection with the top end cover through the clamping ring 111, so that the middle plastic gasket 112 and the acrylic transparent plate 11 are propped against the top end cover, and sealing connection is realized. The pressure transmitter 3 and the temperature and humidity sensor 6 are directly connected to the top end cover through threads. The PI heating film is stuck on the top end cover through glue, and the temperature is controlled through a PI heating film temperature controller.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, or alternatives falling within the spirit and principles of the invention.

Claims (9)

1. A microscope is with multi-functional tissue environment culture apparatus which characterized in that: comprises a multifunctional sealing end cover (1) and a culture dish (2), wherein the culture dish (2) comprises a bottom matrix (21), a replaceable slide (22) and a slide fixing device; a slide fixing device is arranged in the middle of the bottom matrix (21), and a replaceable slide (22) is fixed on the slide fixing device; the multifunctional sealing end cover (1) is arranged above the culture dish (2), a through hole is formed in the middle of the top surface of the multifunctional sealing end cover (1), an acrylic transparent plate (11) is arranged in the through hole, a PI heating film (12) is arranged at the bottom of the through hole, the PI heating film (12) is positioned right above the replaceable glass slide (22), and a power line of the PI heating film (12) penetrates out of the side wall of the multifunctional sealing end cover (1); the side wall of the multifunctional sealing end cover (1) is provided with a pressure transmitter (3), a pressure regulating device (4), an air pipe joint (5) and a temperature and humidity sensor (6); the air pipe joint (5) is connected with an ultrasonic heater; the pressure transmitter (3) and the temperature and humidity sensor (6) are connected with a paperless recorder.
2. A multi-functional tissue environment culture device for a microscope as claimed in claim 1, wherein: slide fixing device includes fixed iron sheet (231), fixed magnet (232) and sealing washer (233), the middle part of bottom base member (21) is equipped with slide mounting hole (211), the below of slide mounting hole (211) is equipped with fixed iron sheet (231), the top of slide mounting hole (211) is equipped with fixed magnet (232), be equipped with sealing washer (233) between fixed magnet (232) and fixed iron sheet (231), interchangeable slide (22) are located slide mounting hole (211).
3. A multi-functional tissue environment culture device for a microscope as claimed in claim 1, wherein: the bottom of the bottom base body (21) is embedded with a plurality of objective table magnets (212).
4. A multi-functional tissue environment culture device for a microscope as claimed in claim 1, wherein: a plurality of end cover magnets (213) are arranged on the bottom substrate (21), a plurality of culture dish magnets are arranged at the bottom of the multifunctional sealing end cover (1), and the end cover magnets (213) are in one-to-one correspondence with the culture dish magnets in position and have opposite magnetic poles; the multifunctional sealing end cover (1) is fastened with the culture dish (2) through a plurality of locking screws, and a compressed silica gel sealing ring (234) is arranged between the multifunctional sealing end cover (1) and the culture dish (2).
5. A multi-functional tissue environment culture device for a microscope as claimed in claim 1, wherein: pressure regulating device (4) are including air-vent valve body (41), sealing block (42), spring (43) and pressure regulating nut (44), inside cavity (411) that are equipped with of air-vent valve body (41), the one end of cavity (411) is equipped with links up pipeline (412), the other end intercommunication culture apparatus's of linking pipeline (412) inside, the other end intercommunication external environment, be close to culture apparatus's one end in cavity (411) is equipped with sealing block (42), the external diameter of sealing block (42) is greater than the internal diameter of linking pipeline (412), the one end that is close to external environment in cavity (411) is equipped with pressure regulating nut (44), be connected through spring (43) between pressure regulating nut (44) and sealing block (42).
6. A multi-functional tissue environment culture device for a microscope as claimed in claim 5, wherein: a guide adapter (45) is arranged between the spring (43) and the sealing block (42), one side of the guide adapter (45) is provided with a sealing block mounting groove, and the other side is provided with a spring mounting groove; one end of the sealing block (42) is fixedly arranged in the sealing block mounting groove, one end of the spring (43) is fixedly arranged in the spring mounting groove, and the outer diameter of the guide adapter (45) is matched with the inner diameter of the cavity (411).
7. A multi-functional tissue environment culture device for a microscope as claimed in claim 5, wherein: the pressure regulating nut (44) is connected with the inner wall of the cavity (411) through threads.
8. A multi-functional tissue environment culture device for a microscope as claimed in claim 1, wherein: the multifunctional sealing end cover (1) is characterized in that a power line sealing nut (121) for sealing the power line of the PI heating film (12) is arranged on the side face of the multifunctional sealing end cover (1), the power line sealing nut (121) is connected with the side face of the multifunctional sealing end cover (1) through threads, and a special-shaped sealing ring (122) is arranged between the power line sealing nut (121) and the multifunctional sealing end cover (1).
9. A multi-functional tissue environment culture device for a microscope as claimed in claim 1, wherein: the lower part of the acrylic transparent plate (11) is provided with a clamping ring (111), the clamping ring (111) is fixedly connected with the through hole through threads, and a gasket (112) is arranged between the acrylic transparent plate (11) and the clamping ring (111).
CN202311509776.1A 2023-11-14 2023-11-14 Microscope is with multi-functional tissue environment culture apparatus Pending CN117448162A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202311509776.1A CN117448162A (en) 2023-11-14 2023-11-14 Microscope is with multi-functional tissue environment culture apparatus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202311509776.1A CN117448162A (en) 2023-11-14 2023-11-14 Microscope is with multi-functional tissue environment culture apparatus

Publications (1)

Publication Number Publication Date
CN117448162A true CN117448162A (en) 2024-01-26

Family

ID=89590809

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Application Number Title Priority Date Filing Date
CN202311509776.1A Pending CN117448162A (en) 2023-11-14 2023-11-14 Microscope is with multi-functional tissue environment culture apparatus

Country Status (1)

Country Link
CN (1) CN117448162A (en)

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