CN117448161A - Live cell culture system for microscope - Google Patents

Live cell culture system for microscope Download PDF

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Publication number
CN117448161A
CN117448161A CN202311509772.3A CN202311509772A CN117448161A CN 117448161 A CN117448161 A CN 117448161A CN 202311509772 A CN202311509772 A CN 202311509772A CN 117448161 A CN117448161 A CN 117448161A
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CN
China
Prior art keywords
end cover
slide
cell culture
connecting pipe
culture system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202311509772.3A
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Chinese (zh)
Inventor
杨懿
陈宇翔
严峻
李增
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Zhejiang Hehu Technology Co ltd
Original Assignee
Zhejiang Hehu Technology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zhejiang Hehu Technology Co ltd filed Critical Zhejiang Hehu Technology Co ltd
Priority to CN202311509772.3A priority Critical patent/CN117448161A/en
Publication of CN117448161A publication Critical patent/CN117448161A/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/46—Means for fastening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas

Abstract

The invention discloses a living cell culture system for a microscope, which comprises an incubator and a humidifying device, wherein the incubator consists of an end cover and a culture dish, the culture dish comprises a bottom substrate, a slide fixing device is arranged in the middle of the bottom substrate, and a replaceable slide is fixed on the slide fixing device; an end cover is arranged above the culture dish, a through hole is formed in the top surface of the end cover, and a PI heating film is arranged at the bottom of the through hole; a humidifying joint is arranged on the side face of the end cover; the humidifying device comprises a cover plate and a cavity, wherein a carbon dioxide air inlet joint, an air outlet joint and a water inlet joint are arranged on the cover plateThe air outlet connector is connected with the humidifying connector; an ultrasonic atomization sheet is arranged in the cavity. Solves the problems of tightness and volume of a living cell culture system for a domestic large microscope, and has the advantages of applicability, sterile environment, temperature and CO 2 And the concentration is difficult to accurately control.

Description

Live cell culture system for microscope
Technical Field
The invention relates to the technical field of living cell culture system design, in particular to a living cell culture system for a microscope.
Background
Live cell imaging is a research method that observes the movement changes of the internal structures of cells and the physiological processes of cells through image acquisition in real-time and dynamically or over a period of time. Living cell imaging, on the one hand, maintains the physiological activity of cells and, on the other hand, enables real-time observation and image acquisition using a microscope, thus requiring support from a cell culture environment control system. Cell culture environmental control systems are essential components for living cell imaging. Animal cells generally can only maintain normal activity for a short period of time without culture medium or at a suitable temperature. Thus, in order to achieve live cell imaging on a microscope, a culture environment control system must be used to maintain the normal metabolic state of the cells.
Environment and conditions of cell culture: 1. sterile environment: non-toxic and sterile are the primary conditions for in vitro cell culture. The detoxification system and the immune system can resist invasion of microorganisms or other harmful substances in vivo, but the cells lack protection of the immune system of the organism in the process of in vitro culture, so that the defense capability of the cells against microorganisms and the detoxification capability of the harmful substances are lost. To ensure that cells can grow and reproduce in an in vitro environment, sterile working areas, good personal hygiene, sterile reagents and media, and sterile procedures must be ensured.
2. Suitable ambient temperature and humidity and gas concentration: depending on the cell type. Except for the medium conditions under which the cells are mated: insect cells were in a 27 ℃ sterile ambient clean air condition, HEK293 was in a 37 ℃ sterile ambient clean air condition, 5% carbon dioxide; CHO is a sterile ambient clean air condition at 37 ℃,5% carbon dioxide; humidity of 95% or more is required.
There are two types of living cell environmental control systems currently in use: the first is a small-sized foreign control box with the volume of about 300 milliliters, and the system consists of a culture room, a humidifier, a temperature controller and a carbon dioxide gas mixing device which are arranged on an objective table.
The second type is a large incubator body, the volume is about 125 liters, the parts of the incubator body comprise a transparent incubator body covering the whole microscope, temperature and humidity control, air blowing, CO2 concentration control and the like, and the incubator body needs to be tightly attached to the microscope body and is provided with a window for convenient operation.
A first system: 1. in order to ensure that the humidity in the culture room reaches 95%, a humidifier is arranged in the culture room, the humidifier generates water mist in a heating and evaporating mode, and the water mist can be evaporated upwards and stuck on a transparent plastic plate, so that the observation effects of a microscope DIC and transmitted light which are arranged in the culture room are seriously affected; 2. compared with a foreign carbon dioxide culture system, the humidifier is placed in the culture chamber, the water quantity is insufficient due to the problem of space storage, the humidity cannot be kept for a long time, and if water addition is needed, the culture chamber must be re-opened, so that the experiment cannot be completely carried out. 3. Related patents and products are not available in China, and the price is generally high, and is generally between 10 and 20 ten thousand.
The domestic patent and the product are basically the second system, 1. The biggest problem is that the tightness is not feasible, because the acrylic plate is assembled on the microscope, because the microscope is provided with a related transmission mechanism, and the shape tolerance of the sheet metal part is hard to control, the acrylic plate is hard to be completely sealed with the microscope, and thus, the temperature and the CO2 concentration are hard to be accurately controlled due to continuous exchange with outside air. 2. Meanwhile, the sterilizing device is large and heavy, is complex to install, is difficult to sterilize after being used, and cannot ensure a sterile environment all the time. 3. The system is generally suitable for a living cell workstation system with specific functions, has complete functions and various requirements as a high-value and high-performance microscope, and therefore, if the large incubator is adopted, the incubator needs to be assembled and disassembled frequently, and the operation has great risk of damaging the incubator body or the instrument.
In summary, these two conventional living cell environmental control systems are limited in application adaptability due to their respective structures.
Disclosure of Invention
The invention aims to provide a living cell culture system for a microscope, which overcomes the defects in the prior art.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the application discloses a living cell culture system for a microscope, which comprises an incubator and a humidifying device, wherein the incubator consists of an end cover and a culture dish, and the culture dish comprises a bottom substrate, a replaceable slide and a slide fixing device; a slide fixing device is arranged in the middle of the bottom matrix, and a replaceable slide is fixed on the slide fixing device; an end cover is arranged above the culture dish, a through hole is formed in the top surface of the end cover, an acrylic transparent plate is arranged in the through hole, a PI heating film is arranged at the bottom of the through hole and is positioned above the replaceable slide, and a hygrothermograph for monitoring the temperature and the humidity inside the end cover is arranged at the top of the end cover; a humidifying joint is arranged on the side face of the end cover; the humidifying device comprises a cover plate and a cavity, wherein a carbon dioxide air inlet joint, an air outlet joint and a water inlet joint are arranged on the cover plate, the cover plate is positioned above the cavity and is in sealing connection with the cavity, and the air outlet joint is connected with the humidifying joint; an ultrasonic atomization sheet is arranged in the cavity.
Preferably, the slide fixing device comprises a fixed iron sheet, a fixed magnet and a sealing ring, wherein a slide mounting hole is formed in the middle of the bottom substrate, the fixed iron sheet is arranged below the slide mounting hole, the fixed magnet is arranged above the slide mounting hole, the sealing ring is arranged between the fixed magnet and the fixed iron sheet, and the replaceable slide is positioned in the slide mounting hole.
Preferably, the bottom of the bottom substrate is embedded with a plurality of objective table magnets.
Preferably, the bottom substrate is provided with a plurality of end cover magnets, the bottom of the end cover is provided with a plurality of culture dish magnets, and the end cover magnets and the culture dish magnets are in one-to-one correspondence and have opposite magnetic poles; and a silica gel sealing ring is arranged between the end cover and the culture dish.
Preferably, the lower extreme of inlet tube is equipped with the connecting pipe, the other end of connecting pipe is equipped with automatic humidification device, automatic humidification device includes base member, tetrafluoro sealing washer and circular foam, be equipped with a plurality of delivery outlet on the base member, the upper end of base member is connected with the connecting pipe, the inside tetrafluoro sealing washer and the circular foam that are equipped with of base member, tetrafluoro sealing washer is located the top of circular foam, tetrafluoro sealing washer is located the mouth of pipe below of connecting pipe.
Preferably, an adapter is arranged between the connecting pipe and the base body, the upper end of the adapter is in threaded connection with the connecting pipe, an output port is arranged in the middle of the adapter, the output port is connected with a pipe orifice of the connecting pipe, the upper end of the base body is in threaded connection with the adapter, and the tetrafluoro sealing ring is located right below the output port.
Preferably, a connecting port is arranged at the bottom of the end cover, the upper end of the connecting pipe is connected with the connecting port through threads, and the bottom of the connecting port is communicated with the lower end of the water inlet pipe.
Preferably, the cover plate and the cavity are in sealing connection through threads and a raw material belt.
The invention has the beneficial effects that:
1. through the mode of design PI heating film below the transparent acrylic plate, solve the problem that the transparent plastic plate can produce water mist in the living cell culture system for the foreign small microscope
2. Solves the problems of tightness and volume of a living cell culture system for a domestic large microscope, and has the advantages of applicability, sterile environment, temperature and CO 2 The concentration is difficult to control accurately;
3. the cost is low, and compared with foreign products, the cost and the price after domestic self-production can be reduced by 10 times;
4. the split design, the bottom base body design is similar to a reusable culture dish, and compared with the conventional disposable plastic culture dish at present, the split design is only needed to replace the cell climbing sheet in the structure, and meanwhile, the split design is not only suitable for the cell climbing sheet, but also can replace the plastic sheet, the cell counting plate and the glass slide, wherein the scribing cover glass is used for separating cultured cells. Compared with the disposable plastic culture dish, the price of the disposable plastic culture dish is 1 piece of 36 pieces of money, so that the cost for long-term use is saved, and the application range is enlarged;
5. the hygrothermograph is directly arranged on the living cell culture system for the microscope, so that a user can intuitively observe the temperature and the humidity of the living cell culture system for the microscope;
6. the magnetic attraction type design can be directly adsorbed on a microscope objective table without installing screws, so that customer experience is improved;
7. independent ultrasonic wave humidification device, for foreign carbon dioxide culture system puts into the cultivate indoor with the humidifier, because the space stores the problem, leads to the water yield insufficient, can't keep humidity for a long time, if need to add water, must reopen cultivate the room, leads to the experiment to go on incompletely.
The features and advantages of the present invention will be described in detail by way of example with reference to the accompanying drawings.
Drawings
FIG. 1 is a schematic view showing the structure of an incubator of a living cell culture system for a microscope according to the present invention;
FIG. 2 is an exploded view of the structure of the end cap of the incubator of the present invention;
FIG. 3 is an exploded view of the structure of a culture dish of the incubator of the present invention;
FIG. 4 is a plan view showing a construction of a humidifying device of a living cell culture system for a microscope according to the present invention;
fig. 5 is a sectional view showing a structure of a humidifying device according to the present invention;
fig. 6 is a schematic structural view of an automatic water adding device of the humidifying device of the present invention;
in the figure: 1-end cover, 11-through hole, 12-acrylic transparent plate, 13-PI heating film, 14-hygrothermograph, 15-humidifying joint, 2-culture dish, 21-bottom base body, 211-mounting hole, 212-end cover magnet, 213-silica gel sealing ring, 214-objective table magnet, 22-replaceable slide, 23-slide fixing device, 231-fixed iron sheet, 232-fixed magnet, 233-sealing ring, 3-cover plate, 31-connecting port, 4-cavity, 5-connecting pipe, 6-automatic humidifying device, 61-base body, 611-water adding port, 62-tetrafluoro sealing ring, 63-circular foam, 7-adapter, 8-ultrasonic atomizing sheet.
Detailed Description
The present invention will be further described in detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the detailed description and specific examples, while indicating the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention. In addition, in the following description, descriptions of well-known structures and techniques are omitted so as not to unnecessarily obscure the present invention.
The invention relates to a living cell culture system for a microscope, which comprises an incubator and a humidifying device, referring to FIG. 1, wherein the incubator consists of an end cover 1 and a culture dish 2, as shown in FIG. 2, the culture dish 2 comprises a bottom substrate 21, a replaceable slide 22 and a slide fixing device 23; the middle part of the bottom matrix 21 is provided with a slide fixing device 23, and a replaceable slide 22 is fixed on the slide fixing device 23 and can be replaced by a cell climbing sheet, a streak cover glass, a plastic sheet, a cell counting plate and a slide glass according to application scenes; an end cover 1 is arranged above the culture dish 2, as shown in fig. 3, a through hole 11 is arranged on the top surface of the end cover 1, an acrylic transparent plate 12 is arranged in the through hole 11, a PI heating film 13 is arranged at the bottom of the through hole 11, the PI heating film 13 is positioned above the replaceable slide 22, a hygrothermograph 14 for monitoring the temperature and humidity inside the end cover 1 is arranged at the top of the end cover 1, and the hygrohygrothermograph 14 is mainly used for monitoring the temperature and humidity conditions in a living cell culture system for a microscope;
the acrylic transparent plate 12 is adhered to the end cover 1 through glue, the PI heating film 13 is positioned under the acrylic transparent plate 12 and also adhered to the end cover 1, the heating and temperature maintaining functions are mainly achieved, the top temperature is slightly higher than the bottom temperature because the heater is positioned at the top, and water mist generally generates condensed water when meeting cold air at high temperature, and the principle is similar to that of an electric heating anti-fog mirror.
A humidifying joint 15 is arranged on the side surface of the end cover 1; the humidifying device comprises a cover plate 3 and a cavity 4, wherein the cover plate 3 is provided with a carbon dioxide air inlet joint, an air outlet joint and an air inlet joint, the cover plate 3 is positioned above the cavity 4 and is in sealing connection with the cavity 4, the air outlet joint is connected with a humidifying joint 15, and an ultrasonic atomizing sheet 8 is arranged inside the cavity 4, as shown in fig. 5.
In one possible implementation, as shown in fig. 3, the slide fixing device 23 includes a fixed iron plate 231, a fixed magnet 232 and a sealing ring 233, a slide mounting hole 211 is formed in the middle of the bottom substrate 21, the fixed iron plate 231 is disposed below the slide mounting hole 211, the fixed magnet 232 is disposed above the slide mounting hole 211, the sealing ring 233 is disposed between the fixed magnet 232 and the fixed iron plate 231, and the replaceable slide 22 is located in the slide mounting hole 211. Specifically, a circular hollow iron sheet (i.e., a fixed iron sheet 231) is adhered under the bottom substrate 21, and mainly provides a magnetic attraction force for the fixed magnet 232, and a sealing ring 233 is arranged on the replaceable slide 304, and is mainly matched with the magnet 301 to play a role in sealing and preventing culture fluid on the replaceable slide 304 from flowing out.
The bottom of the bottom substrate 21 is embedded with a plurality of stage magnets 214, so that the bottom substrate can be directly magnetically attracted onto a microscope stage without screw mounting, and the operation time is saved.
The bottom substrate 21 is provided with a plurality of end cover magnets 212, the bottom of the end cover 1 is provided with a plurality of culture dish magnets, and the end cover magnets 212 and the culture dish magnets are in one-to-one correspondence and have opposite magnetic poles; a silica gel sealing ring 213 is arranged between the end cover 1 and the culture dish 2. Specifically, 4 end cover magnets 212 and a silica gel sealing ring 213 are adhered to the bottom substrate at the same time, so that a magnetic attraction force can be formed between the end cover magnets and four small magnets on the end cover 1, the silica gel sealing ring 303 is compressed, a sealing environment is formed, but a relatively large pressure is required, and when the magnetic attraction force of the magnets is exceeded, four locking screws are additionally required to lock the end cover at the end part so as to form a seal with the bottom substrate.
In a possible embodiment, as shown in fig. 6, the lower end of the water inlet joint is provided with a connecting pipe 5, the other end of the connecting pipe 5 is provided with an automatic humidifying device 6, the automatic humidifying device 6 comprises a base body 61, a tetrafluoro sealing ring 62 and circular foam 63, a plurality of water inlets 611 are formed in the base body 61, the upper end of the base body 61 is connected with the connecting pipe 5, the tetrafluoro sealing ring 62 and the circular foam 63 are arranged in the base body 61, the tetrafluoro sealing ring 62 is located above the circular foam 63, and the tetrafluoro sealing ring 62 is located below a pipe orifice of the connecting pipe 5.
The connecting pipe is characterized in that an adapter 7 is arranged between the connecting pipe 5 and the base body 61, the upper end of the adapter 7 is in threaded connection with the connecting pipe 5, an output port is arranged in the middle of the adapter 7 and is connected with a pipe orifice of the connecting pipe 5, the upper end of the base body 61 is in threaded connection with the adapter 7, and the tetrafluoro sealing ring 62 is located right below the output port. The bottom of apron 3 is equipped with connector 31, pass through threaded connection between the upper end of connecting pipe 5 and the connector 31, the bottom of connector 31 is linked together with the lower extreme of water inlet joint.
In a possible embodiment, the cover plate 3 and the cavity 4 are connected in a sealing manner by threads and a raw material belt.
Specifically, ultrasonic wave humidification device has 3 quick-operation joint through threaded connection on the apron 3, is respectively: CO 2 The air inlet, the air outlet and the purified water inlet of the gas; the cover plate 3 and the cavity 61 are connected in a sealing manner by threads and a raw material tape. The stainless steel pipe 7 with threads is connected to the cover plate 3 through threads under the quick connector 4 at the water inlet, and meanwhile, the stainless steel pipe (the connecting pipe 5) with threads and the automatic water adding device 6 are connected through threads of the adapter 7. The adapter 7 and the base body 61 are connected by threads. Meanwhile, the tetrafluoro sealing ring 62 and the circular foam 63 are arranged in the matrix 61 of the water adding device, when the water is full, the circular foam 63 has an upward buoyancy, and the tetrafluoro sealing ring 62 is propped against to realize sealing, so that the water cannot flow in through the quick connector. When the water level is insufficient, the tetrafluoro seal ring 62 and the circular foam 63 drop, the seal fails, and the water directly flows down and flows into the humidifier chamber 4 through the through hole of the water adding device base 61. The precondition of automatic water adding is that the air pipe joint of the water inlet is required to be always connected with a water tap, and the water tap is in a normally open state. If only for one-time water addition, the ball valve can be closed to form a seal after water addition, and the ultrasonic atomization sheet 8 floats on the water surface.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, or alternatives falling within the spirit and principles of the invention.

Claims (8)

1. A living cell culture system for a microscope, characterized in that: the culture dish comprises an incubator and a humidifying device, wherein the incubator consists of an end cover (1) and a culture dish (2), and the culture dish (2) comprises a bottom base body (21), a replaceable slide (22) and a slide fixing device (23); a slide fixing device (23) is arranged in the middle of the bottom base body (21), and a replaceable slide (22) is fixed on the slide fixing device (23); an end cover (1) is arranged above the culture dish (2), a through hole (11) is formed in the top surface of the end cover (1), an acrylic transparent plate (12) is arranged in the through hole (11), a PI heating film (13) is arranged at the bottom of the through hole (11), the PI heating film (13) is arranged above the replaceable glass slide (22), and a hygrothermograph (14) for monitoring the temperature and the humidity inside the end cover (1) is arranged at the top of the end cover (1); a humidifying joint (15) is arranged on the side surface of the end cover (1); the humidifying device comprises a cover plate (3) and a cavity (4), wherein a carbon dioxide air inlet joint, an air outlet joint and a water inlet joint are arranged on the cover plate (3), the cover plate (3) is positioned above the cavity (4) and is in sealing connection with the cavity (4) and the cavity and the air outlet joint, the air outlet joint is connected with a humidifying joint (15), and an ultrasonic atomizing sheet (8) is arranged inside the cavity (4).
2. A viable cell culture system for microscopes as claimed in claim 1, wherein: slide fixing device (23) are including fixed iron sheet (231), fixed magnet (232) and sealing washer (233), the middle part of bottom base member (21) is equipped with slide mounting hole (211), the below of slide mounting hole (211) is equipped with fixed iron sheet (231), the top of slide mounting hole (211) is equipped with fixed magnet (232), be equipped with sealing washer (233) between fixed magnet (232) and fixed iron sheet (231), interchangeable slide (22) are located slide mounting hole (211).
3. A viable cell culture system for microscopes as claimed in claim 1, wherein: the bottom of the bottom base body (21) is embedded with a plurality of objective table magnets (214).
4. A viable cell culture system for microscopes as claimed in claim 1, wherein: a plurality of end cover magnets (212) are arranged on the bottom base body (21), a plurality of culture dish magnets are arranged at the bottom of the end cover (1), and the end cover magnets (212) are in one-to-one correspondence with the culture dish magnets in position and have opposite magnetic poles; a silica gel sealing ring (213) is arranged between the end cover (1) and the culture dish (2).
5. A viable cell culture system for microscopes as claimed in claim 1, wherein: the lower extreme of water inlet connector is equipped with connecting pipe (5), the other end of connecting pipe (5) is equipped with automatic humidification device (6), automatic humidification device (6) are including base member (61), tetrafluoro sealing washer (62) and circular foam (63), be equipped with a plurality of filler (611) on base member (61), the upper end of base member (61) is connected with connecting pipe (5), inside tetrafluoro sealing washer (62) and the circular foam (63) of being equipped with of base member (61), tetrafluoro sealing washer (62) are located the top of circular foam (63), tetrafluoro sealing washer (62) are located the mouth of pipe below of connecting pipe (5).
6. A viable cell culture system for microscopes as claimed in claim 5, wherein: be equipped with adapter (7) between connecting pipe (5) and base member (61), adopt threaded connection between the upper end of adapter (7) and connecting pipe (5), the middle part of adapter (7) is equipped with the delivery outlet, the delivery outlet links up with the mouth of pipe of connecting pipe (5), adopt threaded connection between the upper end of base member (61) and adapter (7), tetrafluoro sealing washer (62) are located under the delivery outlet.
7. A viable cell culture system for microscopes as claimed in claim 5, wherein: the bottom of apron (3) is equipped with connector (31), pass through threaded connection between the upper end of connecting pipe (5) and connector (31), the bottom of connector (31) is linked together with the lower extreme of water inlet joint.
8. A viable cell culture system for microscopes as claimed in claim 1, wherein: the cover plate (3) is connected with the cavity (4) in a sealing way through threads and a raw material belt.
CN202311509772.3A 2023-11-14 2023-11-14 Live cell culture system for microscope Pending CN117448161A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202311509772.3A CN117448161A (en) 2023-11-14 2023-11-14 Live cell culture system for microscope

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202311509772.3A CN117448161A (en) 2023-11-14 2023-11-14 Live cell culture system for microscope

Publications (1)

Publication Number Publication Date
CN117448161A true CN117448161A (en) 2024-01-26

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202311509772.3A Pending CN117448161A (en) 2023-11-14 2023-11-14 Live cell culture system for microscope

Country Status (1)

Country Link
CN (1) CN117448161A (en)

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