CN117405914A - Quantitative transfer method and system for centrifugal microfluidic reagent - Google Patents
Quantitative transfer method and system for centrifugal microfluidic reagent Download PDFInfo
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 190
- 238000012546 transfer Methods 0.000 title claims abstract description 151
- 238000000034 method Methods 0.000 title claims abstract description 66
- 238000001514 detection method Methods 0.000 claims abstract description 94
- 238000001228 spectrum Methods 0.000 claims abstract description 69
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 66
- 239000002265 redox agent Substances 0.000 claims abstract description 55
- 230000008569 process Effects 0.000 claims abstract description 37
- 238000005259 measurement Methods 0.000 claims abstract description 27
- 238000007781 pre-processing Methods 0.000 claims description 12
- 239000011159 matrix material Substances 0.000 claims description 9
- 238000012545 processing Methods 0.000 claims description 6
- 230000009471 action Effects 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 230000033116 oxidation-reduction process Effects 0.000 claims description 3
- 238000011002 quantification Methods 0.000 abstract description 25
- 239000007788 liquid Substances 0.000 description 26
- 230000001133 acceleration Effects 0.000 description 7
- 238000010586 diagram Methods 0.000 description 5
- 230000036772 blood pressure Effects 0.000 description 4
- 238000004891 communication Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000003638 chemical reducing agent Substances 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- G—PHYSICS
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- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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Abstract
The invention discloses a quantitative transfer method and a quantitative transfer system for a centrifugal microfluidic reagent, wherein the method comprises the following steps: pre-storing standard map data of a detection pool area after finishing different reagent transfer stages; in the reagent transferring process of the disk chip, collecting actual measurement map data of a detection pool area in different reagent transferring stages in real time; calculating the related errors of the preprocessed actually measured spectrum data and the corresponding standard spectrum data respectively; and controlling the centrifugal rotating speed in different reagent transferring stages according to the related errors until each reagent transferring stage is completed. According to the invention, actually measured spectrum data in the reagent transferring process is collected under the observation of the linear CCD sensor, the actually measured spectrum data is compared with the standard spectrum data after the quantification is completed, and the centrifugal rotating speed is controlled according to the size of the related error, so that the quantitative transfer of the reagent is controlled, and the quantitative accuracy of the water sample and the accuracy of the mixing amount of the water sample and the redox agent are improved.
Description
Technical Field
The invention belongs to the technical field of microfluidic water quality detection, and particularly relates to a quantitative transfer method and a quantitative transfer system for a centrifugal microfluidic reagent.
Background
In the field of centrifugal microfluidic water quality detection, a disk chip is used as a carrier for water sample detection and is increasingly widely applied to different fields such as chemistry and chemical industry, environmental monitoring, signal detection and the like. According to different water sample indexes, different redox agents can be packaged in the tray chip in advance, and the water sample and the redox agents are mixed in the detection pool by matching with the puncturing and centrifugal rotating speed modes of the thimble structure in the tray chip. During the mixing process, reagent quantification is particularly important, and the accuracy of the photoelectric detection is determined by the magnitude of the quantification error.
For chemical reagents such as ammonia nitrogen, total phosphorus and the like, reagent quantification is mainly divided into water sample quantification, water sample and redox agent A quantification, and water sample and redox agent A and redox agent B quantification. For total nitrogen, reagent quantification is mainly divided into water sample quantification, water sample and redox agent A quantification. No matter what kind of chemical reagent is used for quantification, the water sample and the redox agent are mixed according to the national standard method. In practical cases, the water sample is inaccurate in the quantitative process of centrifugal transfer due to factors such as hydrophobicity of a disc chip material, valve processing technology, viscosity of different water samples and the like. In particular, the transfer amount of the reagent in the flow channel in the disk chip is controlled by the fixed centrifugal speed and the fixed centrifugal duration, and the transfer amount is often excessive or small, and the concentration detection accuracy is affected by the excessive or small amount of the reagent, which is strictly required in the ratio of the chemical reagent to the corresponding redox reagent or color-developing reagent.
The invention patent with publication number of CN 116400032A discloses a microfluidic water quality detection method, which drives a sample adding reagent to be mixed with a detected water sample on a disk chip through centrifugal driving, when the centrifugal rotation speed is small, the sample adding reagent enters a quantifying pond, when the centrifugal rotation speed is gradually increased, the sample adding reagent gradually enters a detection area, although reagent quantification can be prefabricated, the centrifugal rotation speed cannot be accurately controlled to realize quantitative addition of the reagent, and the applicable scene is limited. The invention patent with publication number of CN114887678A discloses a microfluidic mixed chip for multistage quantitative transfer of various liquids, which realizes quantitative transfer and mixing of reagents through a special structure, but has higher chip replacement cost than a more common disk chip.
Disclosure of Invention
In view of the above, the invention provides a quantitative transfer method and a quantitative transfer system for a centrifugal microfluidic reagent, which are used for solving the problem that the transfer amount of the reagent in a flow channel in a disc chip cannot be accurately controlled in the prior art.
The invention discloses a centrifugal microfluidic reagent quantitative transfer method, which comprises the following steps:
pre-storing standard map data of a detection pool area after finishing different reagent transfer stages;
in the reagent transferring process of the disk chip, collecting actual measurement map data of a detection pool area in different reagent transferring stages in real time;
respectively preprocessing standard map data and actual measurement map data of the detection pool area;
calculating the related errors of the preprocessed actually measured spectrum data and the corresponding standard spectrum data respectively;
and controlling the centrifugal rotating speed in different reagent transferring stages according to the related errors until each reagent transferring stage is completed.
On the basis of the technical scheme, the reagent transferring process of the tray chip preferably comprises a water sample transferring stage, a redox agent A transferring stage and a redox agent B transferring stage, or comprises a water sample transferring stage and a redox agent A transferring stage.
On the basis of the technical scheme, preferably, a linear CCD sensor is adopted to collect actual measurement map data of the detection pool area under different reagent transfer stages, and the collection frequency of the actual measurement map data is the same as the centrifugal rotation speed of the corresponding reagent transfer stage.
On the basis of the above technical solution, preferably, the preprocessing of the standard map data and the actually measured map data of the detection pool area respectively specifically includes:
aiming at standard map data and actual measurement map data in different reagent transfer stages, removing data points outside a detection pool area according to the size of the detection pool area, and storing the map data in the detection pool area in a pixel matrix form of R rows and C columns;
and carrying out binarization processing on the map data in the detection pool area.
On the basis of the above technical solution, preferably, the calculating the correlation error between the preprocessed actually measured spectrum data and the corresponding standard spectrum data specifically includes:
combining each column of the preprocessed standard spectrum data into R-bit binary data, and summing the combined results of each column to obtain standard spectrum characteristic values in different reagent transfer stages;
combining each column of the preprocessed actually measured spectrum data into R-bit binary data, and summing the combined results of each column to obtain actually measured spectrum characteristic values in different reagent transfer stages;
calculating the ratio of the measured spectrum characteristic value to the standard spectrum characteristic value in the corresponding reagent transfer stage, and taking the ratio as a related error.
On the basis of the above technical solution, preferably, the centrifugal rotational speed control under different reagent transfer stages according to the related error specifically includes:
measuring critical rotation speeds of breakthrough valves on flow channels corresponding to different reagent transfer stages in advance, and taking the critical rotation speeds as constant centrifugal rotation speeds of the corresponding reagent transfer stages respectively;
transferring the reagent at constant centrifugal rotation speed;
the duration of the constant centrifugation speed is calculated separately from the associated errors of the different reagent transfer phases.
On the basis of the technical proposal, the constant centrifugal rotating speed is preferably V in the water sample transferring stage 1 Oxygen, oxygenThe transfer stage of the reducing agent A is carried out, and the constant centrifugal rotating speed is V 2 The oxidation-reduction agent B transferring stage, the constant centrifugal rotating speed is V 3 Then: v (V) 1 ＞V 2 ＞V 3 。
On the basis of the above technical solution, preferably, the calculating the duration of the constant centrifugal rotational speed according to the related errors of the different reagent transferring stages specifically includes:
let the related error of different reagent transfer stages be DeltaS n Maintain a constant centrifugal rotational speed V n Is of duration deltat n N=1, 2,3 represent the water sample transfer stage, the redox agent a transfer stage and the redox agent B transfer stage, respectively;
in different reagent transferring stages, constant centrifugal speed V is maintained n Duration Δt of (2) n The calculation process of (1) is as follows:
when 1-DeltaS n When not less than 0, deltat n ＝(1-ΔS n )*T n ；
When 1-DeltaS n When < 0, Δt n ＝0；
Wherein T is n The time required for a standard transfer procedure for the corresponding reagent transfer phase.
In a second aspect of the invention, a centrifugal microfluidic reagent quantitative transfer system is disclosed, the system comprising:
centrifugal microfluidic structure: the device is used for transferring the reagent by taking the disc chip as a carrier under the action of centrifugal force;
linear CCD sensor: the device is used for collecting actual measurement map data of a detection pool area in different reagent transfer stages in real time in the reagent transfer process of the disk chip;
SRAM memory: the method is used for pre-storing standard map data of the detection pool area after the transfer stage of different reagents is finished;
the MCU processor: the method comprises the steps of respectively preprocessing standard map data and actual measurement map data of a detection pool area; calculating the related errors of the preprocessed actually measured spectrum data and the corresponding standard spectrum data respectively; and controlling the centrifugal rotating speed in different reagent transferring stages according to the related errors until each reagent transferring stage is completed.
Compared with the prior art, the invention has the following beneficial effects:
1) According to the invention, actually measured spectrum data in the reagent transferring process is collected under the observation of the linear CCD sensor, related error calculation is carried out on the actually measured spectrum data and the standard spectrum data after quantification is completed, and the rotating speed of each reagent transferring stage is controlled according to the magnitude of the related error, so that the quantitative transfer of the reagent is controlled, and the quantitative accuracy of a water sample and the accuracy of the mixing amount of the water sample and the redox agent are improved.
2) According to the invention, the critical rotation speed of the breakthrough valve on the flow channel corresponding to different reagent transfer stages is used as the corresponding constant centrifugal rotation speed, reagent transfer is carried out at the constant centrifugal rotation speed, and the progress of the reagent quantitative process is judged according to the ratio of the measured spectrum data to the standard spectrum data after the quantitative completion, so that the duration time required by the constant centrifugal rotation speed is calculated, and the accurate control of the reagent transfer quantity is realized.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a centrifugal microfluidic reagent quantitative transfer system according to the present invention;
fig. 2 is a schematic view of a centrifugal microfluidic structure 1 of the present invention;
fig. 3 is a schematic structural view of the disk chip 2;
fig. 4 is an enlarged partial view of the X portion of the disk chip 2;
FIG. 5 is a schematic diagram of a data acquisition and preprocessing process;
fig. 6 is a schematic diagram of the binarization process.
Detailed Description
The following description of the embodiments of the present invention will clearly and fully describe the technical aspects of the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, are intended to fall within the scope of the present invention.
Referring to fig. 1, the present invention proposes a centrifugal microfluidic reagent quantitative transfer system, the system comprising: a centrifugal microfluidic structure 100, a linear CCD sensor 200, an SRAM memory 300 and an MCU processor 400.
The centrifugal microfluidic structure 100 is used for transferring reagents under the action of centrifugal force by taking a disc chip as a carrier.
Fig. 2 is a schematic view of a centrifugal microfluidic structure, which mainly includes a centrifugal disk 1 and a disk chip 2, where the disk chip 2 is fixed on the centrifugal disk 1 and can rotate along with the centrifugal disk 1. Fig. 3 is a schematic diagram of the structure of the disk chip 2 on which the reagent port 21, the liquid sac tank a22, the liquid sac tank B23, the detection tank 24, the quantification tank 25, the waste liquid tank 26, the first flow channel 27, the second flow channel 28, and the third flow channel 29 are provided. The liquid bag of the liquid bag pool A22 is internally provided with a redox agent A, the liquid bag of the liquid bag pool B23 is internally provided with a redox agent B, and ejector pins are respectively arranged in the liquid bag pool A22 and the liquid bag pool B23 and used for puncturing the liquid bag to enable the reagent to flow out. The quantitative reservoir 25, the sac reservoir A22 and the sac reservoir B23 are respectively communicated with the detection reservoir 24 through a first flow channel 27, a second flow channel 28 and a third flow channel 29. Wherein, a first breakthrough valve 271 is arranged at one end of the first flow channel 27 near the detection cell 24, and the first flow channel 27 is used for controlling the chemical reagent in the quantitative cell 25 to be transferred into the detection cell 24; a second breakthrough valve 281 is arranged at one end of the second flow channel 28, which is close to the detection cell 24, and the second flow channel 28 is used for controlling the reagent in the liquid sac cell A22 to be transferred into the detection cell 24; a third breakthrough valve 291 is arranged on one end of the third flow channel 29, which is close to the detection cell 24, and the third flow channel 28 is used for controlling the reagent in the liquid sac cell B23 to be transferred into the detection cell 24.
Fig. 3 is a partial enlarged view of the portion X in fig. 3, where the first breakthrough valve 271, the second breakthrough valve 281, and the third breakthrough valve 291 each have a fixed critical rotation speed, and the critical rotation speed of the first breakthrough valve > the critical rotation speed of the second breakthrough valve > the critical rotation speed of the third breakthrough valve, and only when the centrifugal rotation speed is equal to or higher than the corresponding critical rotation speed, the centrifugal force can cause the reagent to break through the resistance of the corresponding breakthrough valve, thereby conducting the flow path. In the present invention, when the rotation speed of the centrifugal disk 1 is lower than the critical rotation speed of the first breakthrough valve, the first flow channel 27 is not conducted; when the rotational speed reaches or exceeds the threshold rotational speed, the passage of the first flow path 27 is conducted, and the liquid in the dosing tank 25 starts to enter the detection tank 24. The second breakthrough valve 28 and the third breakthrough valve 29 are the same, and only when the rotation speed is greater than or equal to the corresponding critical rotation speed, the centrifugal force can enable the reagent to break through the valve resistance, the corresponding flow channel can be conducted, and the redox agent in the liquid sac pool A22 or the liquid sac pool B23 can enter the detection pool. The invention controls the rotating speed of different reagent transferring stages based on the principle and realizes the quantitative transfer of the reagent.
The linear CCD sensor 200 is fixed above the detection cell 24 and is used for collecting actual measurement map data of the detection cell area in different reagent transfer stages in real time in the reagent transfer process of the disk chip 2.
In this embodiment, the reagent transfer of the tray chip 2 includes a water sample transfer stage, a redox reagent A transfer stage, and a redox reagent B transfer stage, which are three reagent transfer stages in total. In the water sample transferring stage, the water sample in the quantifying tank enters the detecting tank 24 under the action of centrifugal force, after the water quantity is quantified, the water sample enters the redox agent A transferring stage, at the moment, a thimble in the liquid sac tank A22 punctures a liquid sac, the redox agent A stored in the liquid sac tank A22 flows into the detecting tank to be mixed with the water sample in the detecting tank 24, after the water sample and the redox agent A are quantified, the water sample enters the redox agent B transferring stage, at the moment, the thimble in the liquid sac tank B23 punctures a liquid sac, and the redox agent B stored in the liquid sac tank B23 flows into the detecting tank 24 to be mixed with the water sample and the redox agent A.
The SRAM memory 300 is configured to store in advance standard map data of the detection cell area after completion of the different reagent transfer phases;
the MCU processor 400 is used for respectively preprocessing the standard map data and the actual measurement map data of the detection pool area; calculating the related errors of the preprocessed actually measured spectrum data and the corresponding standard spectrum data respectively; and controlling the centrifugal rotating speed in different reagent transferring stages according to the related errors until each reagent transferring stage is completed.
The MCU processor 400 specifically includes the following modules:
and a pretreatment module: and the data points outside the detection pool area are removed, the map data in the detection pool area are reserved, binarization processing is carried out, and the data points are stored in the SRAM 300.
And a related error calculation module: and the method is used for respectively combining each column of the preprocessed standard spectrum data into R-bit binary data, and summing the combined results of each column to obtain the standard spectrum characteristic values in different reagent transfer stages. And respectively combining each column of the preprocessed actually measured spectrum data into R-bit binary data, and summing the combined results of each column to obtain actually measured spectrum characteristic values in different reagent transfer stages. Calculating the ratio of the measured spectrum characteristic value to the standard spectrum characteristic value in the corresponding reagent transfer stage, and taking the ratio as a related error.
Specifically, since the preprocessing module has binarized the elements in the standard map data into 0 and 1, for the pixel matrix of R rows and C columns, if r=32, there are 32 data in each column, and each column is composed of 0 and 1, these 32 data are combined in the order from top to bottom to obtain a 32-bit binary data, the standard map data is changed into a matrix of 1 row and C column, and the combination result of each column is Y n Representing Y n (1, c) represents the elemental subscripts of column c thereof, n=1, 2,3 representing the different reagent transfer phases. Summing the combined results of each column to obtain standard spectrum characteristic values S under different reagent transfer stages n n ：
Similar to the calculation mode of the standard map characteristic value, the elements of the measured map data are combined according to columns to obtain a matrix X of 1 row and C columns n Each is then combined withCombined result X of one column n (1, c) summing to obtain the characteristic value S 'of the actual measurement map under different reagent transfer stages n' n ：
Finally, calculating the ratio of the measured spectrum characteristic value to the standard spectrum characteristic value in the corresponding reagent transfer stage, taking the ratio as a correlation error, and setting the correlation error corresponding to different reagent transfer stages as delta S n Then
Since the map data in the detection cell region reflects the amount of reagent transferred in the detection cell region, Δsn=0 when no reagent is transferred to the detection cell; as the reagent gradually enters the detection cell area, the value of Δsn gradually becomes larger; when the reagent has been completely transferred to the detection cell, Δsn is close to 1.
The rotating speed control module is used for: the reagent transfer device is used for measuring critical rotation speeds of breakthrough valves on flow channels corresponding to different reagent transfer stages in advance, wherein the critical rotation speeds are respectively used as constant centrifugal rotation speeds of the corresponding reagent transfer stages, and reagent transfer is carried out at the constant centrifugal rotation speeds. The duration of the constant centrifugation speed is calculated separately from the associated errors of the different reagent transfer phases.
The invention collects the region of the detection pool 24 of the centrifugal microfluidic structure 100 into the MCU processor 400 in the form of pixel array through the linear CCD sensor 200 by AD, and the MCU processor 400 stores the signals collected by the CCD into the SRAM memory through binarization. Only the data map in the detection pool area needs to be stored in the SRAM memory, and the reading and processing efficiency is high.
On the basis of the centrifugal microfluidic reagent quantitative transfer system, the invention provides a centrifugal microfluidic reagent quantitative transfer method, which comprises the following steps:
s1, pre-storing standard map data of a detection pool area after finishing different reagent transfer stages.
In this example, the reagent transfer stage is divided into a water sample transfer stage, a redox reagent A transfer stage, and a redox reagent B transfer stage. The water sample transfer stage is used for completing water sample quantification, the redox agent A transfer stage is used for completing water sample quantification and redox agent A quantification, and the redox agent B transfer stage is used for completing water sample quantification and redox agent A and redox agent B quantification. The standard spectrum data P1 after the water sample quantification is finished, the standard spectrum data P2 after the water sample and the redox agent A quantification is finished, and the standard spectrum data P3 after the water sample and the redox agent A and the redox agent B quantification is finished are stored in advance.
S2, in the reagent transferring process of the disk chip, actually measured map data of the detection pool area under different reagent transferring stages are collected in real time.
According to the invention, the linear CCD sensor 200 is adopted to collect the actual measurement map data of the detection pool area under different reagent transfer stages, and the collection frequency of the actual measurement map data is the same as the centrifugal rotation speed of the corresponding reagent transfer stage, so that the linear CCD sensor 200 can be used for capturing a photo of the detection pool area once every revolution of the centrifugal disk 1. If the centrifuge disk rotates 13 turns within 1 second, 13 pictures are taken by the linear CCD sensor.
S3, respectively preprocessing the standard map data and the actual measurement map data of the detection pool area.
S31, eliminating data points outside the detection pool area.
The linear CCD sensor 200 is a map image acquired from above the detection cell 24 and the data points beyond the detection cell area of the disk chip 2 may not be stored. Therefore, for standard map data and measured map data in different reagent transfer stages, data points outside the detection cell area can be removed according to the size of the detection cell area, and the map data in the detection cell area can be stored in a pixel matrix form of R rows and C columns.
The linear CCD sensor 200 collects the detection pool area into the MCU processor 400 in the form of a pixel array by using a ratio of detection pool length to width of 3.5:1 as an example, as shown in fig. 5, which is a schematic diagram of a map collection and preprocessing process, the size of the raw map data collected by the linear CCD sensor 200 is 60×125, the detection pool area is distributed in a rectangular area surrounded by (5, 5), (5,117), (37,117) and (37, 5), the number of row pixels is 32, and the number of column pixels is 112, so r=32, c=112 is preferable.
S32, carrying out binarization processing on the map data in the detection pool area.
Let n=1, 2,3 represent water sample transfer stage, redox agent A transfer stage, redox agent B transfer stage respectively, then under a certain transfer stage n, the elements of the r-th row and c-th column in the map data after binarization treatment can be expressed as P n (r, c) =0 or P n (r, c) =1, r e {1,2,..32 }, c e {1,2,..112 }. As shown in fig. 6, the binarization result of the reagent filling area in the detection cell is 1, the binarization result of the cavity area is 0, and when the reagent amount at the position of each pixel point exceeds half of the area of the pixel point, the binarization result is 1, otherwise, the reagent amount is 0.
S4, calculating related errors of the preprocessed actually measured spectrum data and the corresponding standard spectrum data respectively.
S41, respectively combining each column of the preprocessed standard spectrum data into R-bit binary data, and summing the combined results of each column to obtain the standard spectrum characteristic values in different reagent transfer stages.
Since the preprocessing in step S3 has binarized the elements in the standard map data into 0 and 1, for the pixel matrix of R rows and C columns, if r=32, there are 32 data in each column, and they are composed of 0 and 1, and these 32 data are combined in the order from top to bottom to obtain a 32-bit binary data, the standard map data is changed into a matrix of 1 row and C column, and the combined result of each column is used with Y n Representing Y n (1, c) represents the elemental subscripts of column c thereof, n=1, 2,3 representing the different reagent transfer phases.
Summing the combined results of each column to obtain standard spectrum characteristic values S under different reagent transfer stages n n ：
S42, respectively combining each column of the preprocessed actually measured spectrum data into R-bit binary data, and summing the combined results of each column to obtain actually measured spectrum characteristic values in different reagent transfer stages.
Similar to step S41, elements of the measured map data are combined according to columns to obtain a matrix X of 1 row and C columns n Combining the results X of each column n (1, c) summing to obtain the characteristic value S 'of the actual measurement map under different reagent transfer stages n' n ：
S43, calculating the ratio of the measured spectrum characteristic value to the standard spectrum characteristic value in the corresponding reagent transfer stage, and taking the ratio as a related error.
Let the corresponding related error of different reagent transfer stages be DeltaS n Then
Since the map data in the detection cell region reflects the amount of reagent transferred in the detection cell region, Δsn=0 when no reagent is transferred to the detection cell; as the reagent gradually enters the detection cell area, the value of Δsn gradually becomes larger; when the reagent has been completely transferred to the detection cell, Δsn is close to 1.
S5, controlling the centrifugal rotation speed in different reagent transferring stages according to the related errors until each reagent transferring stage is completed.
S51, measuring critical rotation speeds of breakthrough valves on flow channels corresponding to different reagent transfer stages in advance, taking the critical rotation speeds as constant centrifugal rotation speeds of the corresponding reagent transfer stages, and carrying out reagent transfer at the constant centrifugal rotation speeds.
Let the critical rotation speed of the first breakthrough valve 271 beV 1 In the water sample transferring stage, the constant centrifugal speed is V 1 Similarly, let the critical rotation speed of the second breakthrough valve 281 be V 2 The critical rotation speed of the third breakthrough valve 291 is V 3 In the redox agent A transfer stage, the constant centrifugal speed is V 2 In the redox agent B transfer stage, the constant centrifugal speed is V 3 And V is 1 ＞V 2 ＞V 3 。
In this embodiment, V can be set 1 ＝3000rps，V 2 ＝2000rps，V 3 =1000 rps. That is, in the water sample transfer stage, the centrifugal disk 1 is set to rotate at a constant centrifugal speed of 3000rps, at this time, only the first breakthrough valve 271 is opened, the water sample in the quantitative tank enters the detection tank through the first flow channel 27, and as long as the centrifugal speed is lower than 3000rps, the excessive reagent in the quantitative tank is not transferred to the detection tank 24. Similarly, during the redox reagent A transfer phase, the centrifuge disk 1 is set to rotate at a constant centrifuge speed of 2000rps, at which time only the second breakthrough valve 281 is opened and the redox reagent A in the liquid sac tank A22 enters the detection tank 24 through the second flow channel 28 to mix with the water sample. In the redox reagent B transfer phase, the centrifuge disk 1 is set to rotate at a constant centrifuge speed of 1000rps, at which time only the third breakthrough valve 291 is opened, and the redox reagent B in the sac tank B23 enters the detection tank through the third flow channel 29 to be mixed with the water sample and the redox reagent a.
S52, respectively calculating the duration time of the constant centrifugal rotating speed according to the related errors of different reagent transfer stages.
Let the related error of different reagent transfer stages be DeltaS n Maintain a constant centrifugal rotational speed V n Is of duration deltat n N=1, 2,3 represent the water sample transfer stage, the redox agent a transfer stage and the redox agent B transfer stage, respectively;
in different reagent transferring stages, constant centrifugal speed V is maintained n Duration Δt of (2) n The calculation process of (1) is as follows:
when 1-DeltaS n When not less than 0, deltat n ＝(1-ΔS n )*T n ；
When 1-DeltaS n When < 0, Δt n ＝0；
Wherein T is n The time required for a standard transfer procedure for the corresponding reagent transfer phase.
Step S5 is described below in connection with specific experimental data.
In each reagent transferring stage, the whole centrifugal rotating speed operation comprises three processes, namely a process of accelerating from 0 to constant centrifugal rotating speed at the starting time, a process of continuously rotating at the constant centrifugal rotating speed and a process V of decelerating from the constant centrifugal rotating speed to 0 Lowering blood pressure . The continuous rotation process at a constant centrifugal speed is the corresponding reagent transfer process.
Taking the example of a full reagent capacity of 380uL in the detection cell 24, a water sample quantity of 330uL is set, and the required oxidation reducing agent A quantity is: 40uL, required amount of oxidation-reduction agent B: 10uL, the water sample in the detection tank 24 after the completion of water sample quantification is 330uL, the total amount of water sample and redox agent A in the detection tank is 370uL, and the total amount of water sample and redox agent A and redox agent B in the detection tank is 380uL.
In the water sample transfer stage, n=1, the constant centrifugal speed must be greater than or equal to 3000rps to enable the water sample reagent to enter the detection tank 24, and the constant centrifugal speed V is taken 1 ＝3000rps。
Setting the rotational speed V of the process of accelerating from 0 to a constant centrifugal rotational speed Lifting device ＝a 1 *t 1 Wherein a is 1 Is acceleration, t 1 For the duration of acceleration, in order to ensure that the reagent does not generate excessive instantaneous centrifugal force due to excessive acceleration, the reagent is thrown out from the reagent port, a is preferable 1 ＝100，t 1 =30.0 s, then constant centrifugal speed V 1 ＝V Lifting device | t1＝30 =3000 rps. The state of the disk chip at this time is: the water sample reagent begins to pass through the first breakthrough valve 271 and is transferred to the detection cell 24. Collecting actual measurement spectrum data in the water sample transfer process in real time, and calculating related error delta S of the actual measurement spectrum data and standard spectrum data of standard water quantity sample fixing results 1 Calculating a constant centrifugal rotational speed V 1 Duration Δt of rotation 1 ：
When 1-DeltaS 1 When not less than 0, deltat 1 ＝(1-ΔS 1 )*T 1 ；
When 1-DeltaS 1 When < 0, Δt 1 ＝0；
T 1 Is the time required for a standard water sample transfer process.
When DeltaS 1 >1, the deceleration process is started. Process V of speed reduction from constant centrifugal rotor to 0 Lowering blood pressure ＝-a 1 *t 1 When the centrifugal speed is less than 3000rps, the water sample reagent is blocked by the first breakthrough valve 271, and the transfer of the water sample to the detection cell is stopped until the water sample quantification is completed.
In the redox agent a transfer stage, n=2, v' Lifting device ＝a 2 *t 2 Wherein a is 2 Is acceleration, t 2 For the duration of acceleration, let a be 2 ＝100，t 2 =20.0 s, then constant centrifugal speed V 2 ＝V' Lifting device | t2＝20 =2000 rps, when the centrifugal speed V is constant 2 When 2000rps is reached, the ejector pin in the liquid sac pool A22 punctures the liquid sac, and the redox reagent A stored in the liquid sac pool A22 starts to pass through the second breakthrough valve 281 and is transferred to the detection pool 24. Collecting actual measurement spectrum data of the transfer process in real time, and calculating related error delta S of the actual measurement spectrum data and standard spectrum data of quantitative results of the water sample and the redox agent A 2 Calculating a constant centrifugal rotational speed V 2 Duration Δt of rotation 2 ：
When 1-DeltaS 2 When not less than 0, deltat 2 ＝(1-ΔS 2 )*T 2 ；
When 1-DeltaS 2 When < 0, Δt 2 ＝0；
T 2 The time required for a standard transfer process of redox agent a.
When DeltaS 2 >1, the deceleration process is started. V'. Lowering blood pressure ＝-a 2 *t 2 When the centrifugal rotation speed V 2 At less than 2000rps, redox agent A will be blocked by second breakthrough valve 281, stopping transfer to the detection cell, until the water sample + redox agent A quantification is completed.
In the redox agent B transfer stage, n=3, v' Lifting device '＝a 3 *t 3 Wherein a is 3 Is acceleration, t 3 For the duration of acceleration, let a be 3 ＝100，t 3 =10.0 s, then constant centrifugal rotational speedWhen the centrifugal speed V is constant 3 When 1000rps is reached, the ejector pin in the sac pool B pierces the sac, and the redox reagent B stored in the sac pool B starts to pass through the third breakthrough valve 291 and is transferred to the detection cell 24. Collecting actual measurement map data of the transfer process in real time, and calculating related error delta S of the actual measurement map data and standard map data of the stage 3 Calculating a constant centrifugal rotational speed V 3 Duration Δt of rotation 3 ：
When 1-DeltaS 3 When not less than 0, deltat 3 ＝(1-ΔS 3 )*T 3 ；
When 1-DeltaS 3 When < 0, Δt 3 ＝0；
T 3 The time required for a standard transfer process of redox agent B.
When DeltaS 2 >1, start to enter into the deceleration process, speed V Lowering blood pressure ''＝-a 3 *t 3 . When the centrifugal speed is less than 1000rps, the redox agent B is blocked by the third breakthrough valve 291, and the transfer to the detection cell is stopped until the quantitative completion of the water sample and the redox agent A and the redox agent B is completed.
The system embodiments and the method embodiments correspond to each other, and the brief description of the system embodiments is just to refer to the method embodiments.
The invention also discloses an electronic device, comprising: at least one processor, at least one memory, a communication interface, and a bus; the processor, the memory and the communication interface complete communication with each other through the bus; the memory stores program instructions executable by the processor that the processor invokes to implement the aforementioned methods of the present invention.
The invention also discloses a computer readable storage medium storing computer instructions for causing a computer to implement all or part of the steps of the methods of the embodiments of the invention. The storage medium includes: a usb disk, a removable hard disk, a ROM, a RAM, a magnetic disk, or an optical disk, or other various media capable of storing program codes.
The system embodiments described above are merely illustrative, wherein the elements illustrated as separate elements may or may not be physically separate, and the elements shown as elements may or may not be physical elements, i.e., may be distributed over a plurality of network elements. One of ordinary skill in the art may select some or all of the modules according to actual needs without performing any inventive effort to achieve the objectives of the present embodiment.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.
Claims (9)
1. A method for quantitatively transferring a centrifugal microfluidic reagent, the method comprising:
pre-storing standard map data of a detection pool area after finishing different reagent transfer stages;
in the reagent transferring process of the disk chip, collecting actual measurement map data of a detection pool area in different reagent transferring stages in real time;
respectively preprocessing standard map data and actual measurement map data of the detection pool area;
calculating the related errors of the preprocessed actually measured spectrum data and the corresponding standard spectrum data respectively;
and controlling the centrifugal rotating speed in different reagent transferring stages according to the related errors until each reagent transferring stage is completed.
2. The quantitative transfer method of a centrifugal microfluidic reagent according to claim 1, wherein the reagent transfer process of the tray chip comprises a water sample transfer stage, a redox agent a transfer stage and a redox agent B transfer stage.
3. The quantitative transfer method of centrifugal microfluidic reagent according to claim 2, wherein a linear CCD sensor is used to collect the measured spectrum data of the detection cell area in different reagent transfer stages, and the collection frequency of the measured spectrum data is the same as the centrifugal rotation speed of the corresponding reagent transfer stage.
4. The quantitative transfer method of a centrifugal microfluidic reagent according to claim 2, wherein the preprocessing of the standard profile data and the actually measured profile data of the detection cell area respectively specifically comprises:
aiming at standard spectrum data and actual measurement spectrum data in different reagent transferring stages, removing data points outside a detection pool area according to the size of the detection pool area, and storing the standard spectrum data and the actual measurement spectrum data in the detection pool area in a pixel matrix form of R rows and C columns;
and carrying out binarization processing on the standard map data and the actually measured map data in the detection pool area.
5. The quantitative transfer method of a centrifugal microfluidic reagent according to claim 4, wherein calculating the correlation error between the preprocessed measured spectrum data and the corresponding standard spectrum data comprises:
combining each column of the preprocessed standard spectrum data into R-bit binary data, and summing the combined results of each column to obtain standard spectrum characteristic values in different reagent transfer stages;
combining each column of the preprocessed actually measured spectrum data into R-bit binary data, and summing the combined results of each column to obtain actually measured spectrum characteristic values in different reagent transfer stages;
calculating the ratio of the measured spectrum characteristic value to the standard spectrum characteristic value in the corresponding reagent transfer stage, and taking the ratio as a related error.
6. The quantitative transfer method of centrifugal microfluidic reagent according to claim 2, wherein the centrifugal rotational speed control at different reagent transfer stages according to the related error specifically comprises:
measuring critical rotation speeds of breakthrough valves on flow channels corresponding to different reagent transfer stages in advance, and taking the critical rotation speeds as constant centrifugal rotation speeds of the corresponding reagent transfer stages respectively;
transferring the reagent at constant centrifugal rotation speed;
the duration of the constant centrifugation speed is calculated separately from the associated errors of the different reagent transfer phases.
7. The quantitative transfer method of centrifugal microfluidic reagent according to claim 6, wherein the constant centrifugal speed is V 1 In the redox agent A transfer stage, the constant centrifugal rotating speed is V 2 The oxidation-reduction agent B transferring stage, the constant centrifugal rotating speed is V 3 Then: v (V) 1 ＞V 2 ＞V 3 。
8. The method for quantitatively transferring a centrifugal microfluidic reagent according to claim 7, wherein calculating the duration of the constant centrifugal rotational speed according to the related errors of the different reagent transfer stages, respectively, comprises:
let the related error of different reagent transfer stages be DeltaS n Constant centrifugal speed of V n Maintain a constant centrifugal rotational speed V n Is of duration deltat n N=1, 2,3 represent the water sample transfer stage, the redox agent a transfer stage and the redox agent B transfer stage, respectively;
in different reagent transferring stages, constant centrifugal speed V is maintained n Duration Δt of (2) n The calculation process of (1) is as follows:
when 1-DeltaS n When not less than 0, deltat n ＝(1-ΔS n )*T n ；
When 1-DeltaS n When < 0, Δt n ＝0；
Wherein T is n The time required for a standard transfer procedure for the corresponding reagent transfer phase.
9. A centrifugal microfluidic reagent quantitative transfer system, the system comprising:
centrifugal microfluidic structure: the device is used for transferring the reagent by taking the disc chip as a carrier under the action of centrifugal force;
linear CCD sensor: the device is used for collecting actual measurement map data of a detection pool area in different reagent transfer stages in real time in the reagent transfer process of the disk chip;
SRAM memory: the method is used for pre-storing standard map data of the detection pool area after the transfer stage of different reagents is finished;
the MCU processor: the method comprises the steps of respectively preprocessing standard map data and actual measurement map data of a detection pool area; calculating the related errors of the preprocessed actually measured spectrum data and the corresponding standard spectrum data respectively; and controlling the centrifugal rotating speed in different reagent transferring stages according to the related errors until each reagent transferring stage is completed.
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Citations (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1831439A2 (en) * | 2004-12-10 | 2007-09-12 | Simon Fraser University | Microfluidic microarray assemblies and methods of manufacturing and using same |
| CN102645413A (en) * | 2012-03-29 | 2012-08-22 | 东华大学 | Centrifugal microfluidic water quality tester and centrifugal microfluidic water quality test method |
| US20140186963A1 (en) * | 2012-12-28 | 2014-07-03 | Chung Yuan Christian University | Microfluid Testing System with a Multiple-Channel Disc and Utility thereof |
| CN107893026A (en) * | 2017-12-29 | 2018-04-10 | 苏州绘真医学检验所有限公司 | Detect micro-fluidic chip, detection method and the detecting system of human virus' nucleic acid |
| CN109212201A (en) * | 2017-07-03 | 2019-01-15 | 杭州霆科生物科技有限公司 | A kind of centrifugal type microfludic chip for hepatitis B five detections in serum |
| CN112461751A (en) * | 2020-10-16 | 2021-03-09 | 江苏大学 | Cancer cell activity detection and evaluation device and method based on multi-adhesion strength fusion |
| US10981174B1 (en) * | 2016-08-04 | 2021-04-20 | National Technology & Engineering Solutions Of Sandia, Llc | Protein and nucleic acid detection for microfluidic devices |
| CN112871228A (en) * | 2021-01-11 | 2021-06-01 | 中国科学院苏州生物医学工程技术研究所 | Microfluidic detection device for blood type detection |
| CN113155588A (en) * | 2021-05-13 | 2021-07-23 | 武汉新烽光电股份有限公司 | Rapid automatic water quality detection system and method based on micro-fluidic |
| CN114113567A (en) * | 2020-11-26 | 2022-03-01 | 深圳市理邦精密仪器股份有限公司 | Molecular diagnosis centrifugal test card |
| CN114152606A (en) * | 2021-12-02 | 2022-03-08 | 武汉新烽光电股份有限公司 | Water quality monitoring device and monitoring system based on micro-flow control disc chip |
| CN114887678A (en) * | 2022-07-12 | 2022-08-12 | 杭州霆科生物科技有限公司 | Micro-fluidic mixing chip for multi-stage quantitative transfer of various liquids |
| CN217277799U (en) * | 2022-01-24 | 2022-08-23 | 武汉新烽光电股份有限公司 | Portable quick water quality detection system based on micro-flow control disc chip |
| CN115157658A (en) * | 2022-06-23 | 2022-10-11 | 浙江大学 | Method for preparing binary coding droplet array by microfluidic 3D droplet printing |
| CN115254219A (en) * | 2022-09-13 | 2022-11-01 | 杭州霆科生物科技有限公司 | Centrifugal microfluidic detection system and detection control method thereof |
| WO2023059595A1 (en) * | 2021-10-08 | 2023-04-13 | Intabio, Llc | Software for microfluidic systems interfacing with mass spectrometry |
| WO2023108412A1 (en) * | 2021-12-14 | 2023-06-22 | 深圳先进技术研究院 | Rapid recognition and quantification method for target protein |
| CN116400032A (en) * | 2022-12-14 | 2023-07-07 | 武汉新烽光电股份有限公司 | Microfluidic water quality detection method |
| US20230241614A1 (en) * | 2020-09-07 | 2023-08-03 | Shenzhen Yhlo Biotech Co., Ltd. | Droplet microfluidic chip and method for producing microdroplets |
-
2023
- 2023-09-21 CN CN202311231395.1A patent/CN117405914B/en active Active
Patent Citations (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1831439A2 (en) * | 2004-12-10 | 2007-09-12 | Simon Fraser University | Microfluidic microarray assemblies and methods of manufacturing and using same |
| CN102645413A (en) * | 2012-03-29 | 2012-08-22 | 东华大学 | Centrifugal microfluidic water quality tester and centrifugal microfluidic water quality test method |
| US20140186963A1 (en) * | 2012-12-28 | 2014-07-03 | Chung Yuan Christian University | Microfluid Testing System with a Multiple-Channel Disc and Utility thereof |
| US10981174B1 (en) * | 2016-08-04 | 2021-04-20 | National Technology & Engineering Solutions Of Sandia, Llc | Protein and nucleic acid detection for microfluidic devices |
| CN109212201A (en) * | 2017-07-03 | 2019-01-15 | 杭州霆科生物科技有限公司 | A kind of centrifugal type microfludic chip for hepatitis B five detections in serum |
| CN107893026A (en) * | 2017-12-29 | 2018-04-10 | 苏州绘真医学检验所有限公司 | Detect micro-fluidic chip, detection method and the detecting system of human virus' nucleic acid |
| US20230241614A1 (en) * | 2020-09-07 | 2023-08-03 | Shenzhen Yhlo Biotech Co., Ltd. | Droplet microfluidic chip and method for producing microdroplets |
| CN112461751A (en) * | 2020-10-16 | 2021-03-09 | 江苏大学 | Cancer cell activity detection and evaluation device and method based on multi-adhesion strength fusion |
| CN114113567A (en) * | 2020-11-26 | 2022-03-01 | 深圳市理邦精密仪器股份有限公司 | Molecular diagnosis centrifugal test card |
| CN112871228A (en) * | 2021-01-11 | 2021-06-01 | 中国科学院苏州生物医学工程技术研究所 | Microfluidic detection device for blood type detection |
| CN113155588A (en) * | 2021-05-13 | 2021-07-23 | 武汉新烽光电股份有限公司 | Rapid automatic water quality detection system and method based on micro-fluidic |
| WO2023059595A1 (en) * | 2021-10-08 | 2023-04-13 | Intabio, Llc | Software for microfluidic systems interfacing with mass spectrometry |
| CN114152606A (en) * | 2021-12-02 | 2022-03-08 | 武汉新烽光电股份有限公司 | Water quality monitoring device and monitoring system based on micro-flow control disc chip |
| WO2023108412A1 (en) * | 2021-12-14 | 2023-06-22 | 深圳先进技术研究院 | Rapid recognition and quantification method for target protein |
| CN217277799U (en) * | 2022-01-24 | 2022-08-23 | 武汉新烽光电股份有限公司 | Portable quick water quality detection system based on micro-flow control disc chip |
| CN115157658A (en) * | 2022-06-23 | 2022-10-11 | 浙江大学 | Method for preparing binary coding droplet array by microfluidic 3D droplet printing |
| CN114887678A (en) * | 2022-07-12 | 2022-08-12 | 杭州霆科生物科技有限公司 | Micro-fluidic mixing chip for multi-stage quantitative transfer of various liquids |
| CN115254219A (en) * | 2022-09-13 | 2022-11-01 | 杭州霆科生物科技有限公司 | Centrifugal microfluidic detection system and detection control method thereof |
| CN116400032A (en) * | 2022-12-14 | 2023-07-07 | 武汉新烽光电股份有限公司 | Microfluidic water quality detection method |
Non-Patent Citations (7)
| Title |
|---|
| SUN Y, ET AL: "Mircofluidic adaptation of Density-gradient centrifugation for isolation of particles and cells", 《BIOENGINEERING》, vol. 4, no. 3, 30 September 2017 (2017-09-30), pages 67 - 85 * |
| 徐佳男，等: "微流控芯片通道的全息显微检测方法", 《红外与激光工程》, vol. 51, no. 09, 30 December 2021 (2021-12-30), pages 351 - 358 * |
| 朱伟, 等: "光学蛋白质芯片法定量检测C-反应蛋白的研究", 《国际检验医学杂志》, no. 07, 30 July 2007 (2007-07-30), pages 7 - 12 * |
| 李鑫，等: "基于微流控技术的等温核酸即时检测系统", 《中国测试》, vol. 49, no. 11, 28 March 2023 (2023-03-28), pages 7 - 15 * |
| 王华梁，等: "《医学实验室建设与质量管理》", vol. 1, 30 November 2011, 上海科学技术出版社, pages: 308 - 314 * |
| 陈梦月，等: "微流控通道加工技术的研究进展", 《南京航空航天大学机电学院》, vol. 58, no. 03, 15 March 2021 (2021-03-15), pages 244 - 253 * |
| 黄世光，等: "微流控芯片核酸分析系统及其精准医学应用", 《中国激光》, vol. 42, no. 03, 2 January 2018 (2018-01-02), pages 18 - 27 * |
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