CN116400032A - A microfluidic water quality detection method - Google Patents

Abstract

The invention discloses a microfluidic water quality detection method, which specifically includes the following steps: placing a sample: placing a disc chip prefabricated with a water sample for detection and a digestion bottle prefabricated with a reagent for adding samples into the detector from different positions in sequence; Heating digestion: heat and digest the sample reagent in the digestion bottle; transfer mixing: transfer the digested sample reagent to the disk chip, and mix it with the detection water sample prefabricated in the disk chip in advance; detection analysis: transfer to the mixing The final detection water sample emits a detection light source to analyze the data in the detection water sample; in the present invention, the processes of sampling, high-temperature airtight digestion, quantification, and photoelectric detection are all integrated into a sheet with a size of several centimeters through the microfluidic disk chip technology. On the fan-shaped chip, the reagent quantification is all prefabricated, avoiding tedious manual operations, reducing errors, accurate data, micronization of reaction reagents, and less demand for reagents and sample liquids. Compared with conventional laboratory water quality testing, it can be significantly improved. Reduce the amount of waste liquid.

Description

A microfluidic water quality detection method

technical field

The invention relates to the technical field of water quality detection, in particular to a microfluidic water quality detection method.

Background technique

In the traditional water quality testing process, samples are usually taken on-site and brought back to the laboratory for testing. The testing reagents are also manually configured, and then an appropriate amount of testing water samples are added according to testing requirements. Quantitative testing of reagents has not been achieved. The accuracy of the detection data is not high, and it is not conducive to controlling the amount of waste liquid after detection. The mixing of multiple detection reagents is also not conducive to the operation of the staff, which reduces the detection efficiency.

Contents of the invention

The main purpose of the present invention is to provide a microfluidic water quality detection method, aiming to solve the existing technical problems.

In order to achieve the above object, the present invention provides a microfluidic water quality detection method, which specifically includes the following steps,

Sample placement: put the disc chip prefabricated with the detection water sample and the digestion bottle with the prefabricated sample reagent into the detector from different positions in sequence;

Heating digestion: heat and digest the loading reagent in the digestion bottle through the digestion mechanism;

Transfer mixing: transfer the digested loading reagent to the disk chip and mix it with the detection water sample prefabricated in the disk chip;

Detection and analysis: send a detection light source to the mixed detection water sample, and analyze the data in the detection water sample.

Further, during the transfer mixing process, the digested sample addition reagent is transferred to the disc chip, and the sample addition reagent is driven to mix with the detection water sample on the disc chip by centrifugal drive, and the sample addition reagent is quantified and the detection water sample is quantified before mixing. Mixing, when the centrifugation speed is small, the sample reagent enters the quantitative pool, when the centrifugal speed gradually increases, the sample reagent gradually enters the detection area, and the quantitative pool and the detection area are connected through a long and narrow channel.

Further, during the transfer process, the digested digestion bottle is rotated through the transfer mechanism until the mouth of the bottle is facing down, inserted into the disk chip in a vertically downward posture, and stays on the disk chip, and then drives the disk chip to rotate Transposition, the liquid capsule prefabricated on the disk chip is pierced through the piercing mechanism, the detection water sample and the sample reagent flow to mix with each other, and the mixing process is accelerated with the centrifugal action.

Further, the transfer mechanism includes,

The rotating part is used to drive the digestion bottle to change its posture after the digestion reaction is completed, so that the mouth of the digestion bottle is vertically downward;

The vertical moving part is used to drive the digestion bottle to move vertically, so that the digestion bottle after the posture change is moved down and inserted on the disk chip.

Further, during the heating and digestion process of the loading reagent in the digestion bottle, the rotating parts synchronously cooperate to drive the digestion bottle to rotate at a specified speed, so that the loading reagent in it can be fully digested, and then the cooling system is used to cool the loading reagent. Cool down, and then proceed to the subsequent reagent transfer.

Further, during the digestion heating process, the bottom of the digestion bottle is attached to the heating structure, and different digestion reaction temperatures are controlled in real time according to different water samples to be tested through the temperature measuring element.

Further, in the detection and analysis process, the water samples to be inspected are sequentially detected by photoelectric detection modules, and the photoelectric detection modules are arranged at intervals along the centrifugal movement path of the water samples to be inspected. When the module stops, the photoelectric detection module emits light spots of different wavelengths into the water sample to be tested, and automatically identifies the elemental data in the water sample to be tested.

Further, in the process of placing the sample, the digestion bottle and the disk chip are successively placed on the same side of the detector, and the surface of the casing of the detector is provided with a cover door that can be rotatably opened, and the cover door spans a part of the casing. On the upper surface and the front surface, the housing is provided with an integrally formed inner boss near the cover door, and the inner boss is provided with a placement opening for placing a digestion bottle.

The beneficial effects of the present invention are reflected in:

In the present invention, the processes of sampling, high-temperature airtight digestion, quantification, step-by-step mixing of reagents, reaction and color development, and photoelectric detection are all integrated into a fan-shaped chip with a size of several centimeters through the microfluidic disk chip technology. All prefabrication is completed, avoiding tedious manual operations, just add water samples to start testing, reducing errors, accurate data, micro-quantization of reaction reagents, less demand for reagents and sample liquids, comparable to conventional laboratory water quality testing than can significantly reduce the amount of waste liquid.

Description of drawings

Fig. 1 is a schematic diagram of putting in a digestion bottle and a disc chip of the present invention;

Fig. 2 is a schematic diagram of puncturing the liquid sac 1 of the present invention (the liquid sac 1 is punctured in a 90° state of rotation);

Fig. 3 is a schematic diagram of puncturing the liquid capsule 2 of the present invention (the liquid capsule 2 is punctured in a 90° state of rotation);

Fig. 4 is a schematic diagram of insertion of digestion bottle 1 of the present invention (rotated 180 ° state is pressed into digestion bottle 1);

Fig. 5 is a schematic diagram of inserting the digestion bottle 2 of the present invention (the digestion bottle 2 is pressed into the digestion bottle 2 in a state of rotating 180°).

Explanation of reference signs:

100, disc chip; 200, digestion bottle; 300, transfer mechanism; 310, vertical moving part; 320, rotating part; 400, digestion mechanism; 500, photoelectric detection module; 600, piercing mechanism; 700, cover door; case.

Detailed ways

The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. In the case of no conflict, the embodiments in the present application and the features in the embodiments can be combined with each other. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

It should be noted that if there is a directional indication (such as up, down, left, right, front, back...) in the embodiment of the present invention, the directional indication is only used to explain the position in a certain posture (as shown in the accompanying drawing). If the specific posture changes, the directional indication will also change accordingly.

In addition, if there are descriptions involving "first", "second" and so on in the embodiments of the present invention, the descriptions of "first", "second" and so on are only for descriptive purposes, and should not be interpreted as indicating or implying Its relative importance or implicitly indicates the number of technical features indicated. Thus, the features defined as "first" and "second" may explicitly or implicitly include at least one of these features. In addition, the meaning of "and/or" appearing in the whole text includes three parallel schemes, taking "A and/or B" as an example, including scheme A, scheme B, or schemes that both A and B satisfy. In addition, "plurality" means two or more. In addition, the technical solutions of the various embodiments can be combined with each other, but it must be based on the realization of those skilled in the art. When the combination of technical solutions is contradictory or cannot be realized, it should be considered that the combination of technical solutions does not exist .

Please refer to Figures 1-5, a microfluidic water quality detection method of the present invention, specifically comprising the following steps,

Sample placement: put the disc chip 100 prefabricated with the detection water sample and the digestion bottle 200 prefabricated with the sample addition reagent into the detector from different positions in sequence;

Heat digestion: heat and digest the loading reagent in the digestion bottle 200 through the digestion mechanism 400;

Transfer mixing: transfer the digested loading reagent to the disk chip 100, and mix it with the detection water sample prefabricated in the disk chip 100;

Detection and analysis: send a detection light source to the mixed detection water sample, and analyze the data in the detection water sample.

The invention is applicable to on-site water sample detection operations, and the prefabricated reagents are distributed and mixed with the detection water samples for detection, which reduces the operation of artificially adding detection reagents or water samples, improves detection accuracy, and improves detection efficiency.

The present invention standardizedly integrates sampling, high-temperature airtight digestion, quantification, step-by-step mixing of reagents, reaction and color development, photoelectric detection and other processes into a fan-shaped chip with a size of several centimeters through the microfluidic disk chip 100 technology (see the same date for the specific structure. The submitted patent name: a portable microfluidic water quality detector), all prefabricated reagents are quantified, avoiding tedious manual operations, just add water samples to start testing, reducing errors, accurate data, and responsive Reagents are microquantified, and the demand for reagents and sample liquids is small, which can greatly reduce the amount of waste liquid compared with conventional laboratory water quality testing.

In one embodiment, during the transfer mixing process, the digested sample addition reagent is transferred to the disc chip 100, and the sample addition reagent is driven to mix with the detection water sample on the disc chip 100 by centrifugal drive, and the sample addition reagent is quantified and detected before mixing. Quantitative mixing of water samples. When the centrifugal speed is small, the sample reagent enters the quantitative pool. When the centrifugal speed gradually increases, the sample reagent gradually enters the detection area. The quantitative pool and the detection area are connected through a long and narrow channel. In this way, the quantitative addition of reagents can be realized by controlling the centrifuge speed, which helps to add an appropriate amount of reagents according to different detection requirements, and improves the detection accuracy. The reaction is more sufficient, further improving the detection accuracy.

In one embodiment, during the transfer process, the digested digestion bottle 200 is inserted into the disk chip 100 in a vertically downward posture through the rotating part 320 of the transfer mechanism 300 until the bottle mouth is downward, and stays on the disk chip 100 Afterwards, the disc chip 100 is driven to rotate and transpose, and the liquid capsule prefabricated on the disc chip 100 is pierced through the piercing mechanism 600, and the water sample and the reagent to be added are detected to flow and mix with each other, and the mixing process is accelerated with the centrifugal action. In this way, the digestion bottle 200 is left on the disc chip 100, and through the quantitative addition of reagents, the excess waste liquid is retained in the disc chip 100, and the usual laboratory detection waste liquid is turned into solid chip waste, which is convenient for transportation and processing, and Through the alternate operation of the piercing mechanism 600 and the transfer mechanism 300, multiple liquids are mixed step by step, which facilitates the detection reaction.

Due to different detection requirements, it is necessary to mix different reagents and water samples to be tested. During the multi-channel liquid mixing detection process, it is necessary to perform multiple conversion and mixing processes in sequence. Please refer to Figure 2-5 for different digestion bottles. 200 is inserted into different disk chips 100, and the piercing mechanism 600 described below pierces different liquid capsules sequentially to complete the detection of different water samples to be tested.

In one embodiment, the transfer mechanism 300 includes,

The rotating part 320 is used to drive the digestion bottle 200 to change its posture after the digestion reaction is completed, so that the mouth of the digestion bottle 200 is vertically downward;

The vertical moving part 310 is used to drive the digestion bottle 200 to move vertically, so that the digestion bottle 200 after the posture change is moved down and inserted onto the disk chip 100 .

After the digestion bottle 200 and the disk chip 100 are put in and the digestion reaction is completed, the rotation part 320 drives the digestion bottle 200 to change its posture, and transfers it to the disk chip 100 to transfer the reagents, and then cooperates with the rotation part 320 parts, transfer the position of the puncture mechanism 600, puncture the liquid bag on the disk chip 100, realize the mixing of multiple liquids, and the transfer mechanism 300 can change the action to realize the alternate transformation of the position of the digestion bottle 200 and the puncture mechanism 600 and transfer.

In one embodiment, during the heating and digestion process of the loading reagent in the digestion bottle 200, the rotating part 320 is synchronously coordinated to drive the digestion bottle 200 to rotate the part 320 at a specified speed, so that the loading reagent therein can fully undergo a digestion reaction , and then cool down the sample reagent through the heat dissipation system, and then carry out the subsequent reagent transfer. In this way, during the digestion reaction process, the rotation of the digestion bottle 200 is driven by the rotating part 320 to accelerate the digestion reaction rate of the sample-filled reagent, thereby improving the overall detection efficiency, and then cooling down through the heat dissipation system to quickly restore it to The temperature required for subsequent reactions further improves the efficiency of subsequent transfer mixing detection.

Wherein, the heat dissipation system may adopt a heat dissipation pipe and a heat dissipation fan.

In one embodiment, during the digestion and heating process, the bottom of the digestion bottle 200 is attached to the heating structure, and different digestion reaction temperatures are controlled in real time according to different water samples to be tested through the temperature measuring element. In this way, through contact conduction heating, the temperature required for the digestion reaction can be met, and the action when the subsequent digestion bottle 200 is transferred will not be affected, because the upper body of the digestion bottle 200 is in a bare state at this time, which is convenient for the subsequent transfer process. Other limiting structures and contacts; Among them, the heating structure adopts ceramic structure, which can withstand high temperature up to 350°C, and the lead wire uses 0.5mm nickel wire, which has the advantages of fast heating and no power attenuation for long-term use.

In one embodiment, during the detection and analysis process, the water samples to be inspected are sequentially detected by the photoelectric detection module 500, and the photoelectric detection modules 500 are arranged at intervals along the centrifugal movement path of the water samples to be inspected. When the detection module 500 stops, the photoelectric detection module 500 emits light spots of different wavelengths into the water sample to be tested, and automatically identifies the elemental data in the water sample to be tested. The output end of the photoelectric detection module 500 is electrically connected to the data processing module, wherein the data processing module is electrically connected to the touch screen provided on the surface of the box for displaying the detected data. The photoelectric detection module 500 emits light of a specific wavelength, which is captured by the receiving part after passing through the reacting liquid, and can be converted into an electrical signal according to the obtained light signal, and the corresponding value of the concentration can be obtained through a corresponding analysis method. The invention patent CN113655011A "A Microflow Control Detection System and Its Detection Method" applied by our company has disclosed the corresponding photoelectric signal processing circuit.

In one embodiment, during the sample placement process, the digestion bottle 200 and the disk chip 100 are put in successively from the same side of the detector. The surface of the casing 800 of the detector is provided with a cover door 700 that can be turned and opened, and the cover door 700 spans the casing. The upper surface and the front surface of the body 800 are partly provided. The housing 800 is provided with an integrally formed inner boss close to the cover door 700 . The inner boss is provided with a placement opening for placing the digestion bottle 200 . Since the action states of the digestion bottle 200 and the disk chip 100 are different during the detection process, the digestion bottle 200 needs to be moved vertically, while the disk chip 100 only needs to be rotated horizontally. The door 700 can facilitate the taking of the digestion bottle 200 and the disc chip 100 at the same time, and can also facilitate the molding operation of the casing 800, which reduces the molding process and improves the processing efficiency of the detector.

The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included in the scope of the present invention. within the scope of protection.

Claims (8)

1. A microfluidic water quality detection method is characterized in that: in particular comprising the following steps of the method,

sample placement: placing a tray chip (100) prefabricated with a detection water sample and a digestion bottle (200) prefabricated with a sample adding reagent into a detector from different positions in sequence;

heating and digestion: heating and digesting the sample adding reagent in the digestion bottle (200) through a digestion mechanism (400);

transfer mixing: transferring the digested sample adding reagent to a tray chip (100), and mixing the digested sample adding reagent with a detection water sample prefabricated in the tray chip (100) in advance;

detection and analysis: and (3) sending out a detection light source to the mixed detection water sample, and analyzing data in the detection water sample.

2. The microfluidic water quality detection method according to claim 1, wherein: in the transfer mixing process, the digested sample adding reagent is transferred onto the tray chip (100), the sample adding reagent is driven to be mixed with a detected water sample on the tray chip (100) through centrifugal driving, the sample adding reagent is quantitatively mixed with the detected water sample through quantification before mixing, when the centrifugal rotating speed is small, the sample adding reagent enters the quantifying pond, and when the centrifugal rotating speed is gradually increased, the sample adding reagent gradually enters the detecting area, and the quantifying pond is communicated with the detecting area through a long and narrow channel.

3. The microfluidic water quality detection method according to claim 1, wherein: in the transfer process, the digested digestion bottle (200) is rotated to the bottle mouth downwards through the transfer mechanism (300), is inserted onto the tray chip (100) in a vertically downward moving posture and stays on the tray chip (100), then the tray chip (100) is driven to rotate for transposition, the liquid sac prefabricated on the tray chip (100) is punctured through the puncturing mechanism (600), the water sample and the sample adding reagent are detected to flow to be mixed with each other, and the mixing process is accelerated by matching with the centrifugal action.

4. A microfluidic water quality testing method according to claim 3, wherein: the transfer mechanism (300) comprises a plurality of transfer units,

the rotary component (320) is used for driving the digestion bottle (200) to change the posture after the digestion reaction is completed, so that the bottle mouth of the digestion bottle (200) is vertically downward;

and the vertical moving component (310) is used for driving the digestion bottle (200) to move vertically, so that the digestion bottle (200) subjected to posture change moves downwards and is inserted into the tray chip (100).

5. The microfluidic water quality detection method according to claim 4, wherein: in the heating digestion process of the sample adding reagent in the digestion bottle (200), the digestion bottle (200) is driven to rotate at a specified speed through synchronous cooperation of the rotating component (320) so that the sample adding reagent therein fully undergoes digestion reaction, and then the sample adding reagent is cooled through a heat dissipation system, and then subsequent reagent transfer is performed.

6. The microfluidic water quality detection method according to claim 1, wherein: in the digestion heating process, the bottom of the digestion bottle (200) is attached to the heating structure, and different digestion reaction temperatures are controlled in real time according to different water samples to be detected through the temperature measuring element.

7. The microfluidic water quality detection method according to claim 1, wherein: in the detection analysis process, the water sample to be detected is sequentially detected through the photoelectric detection modules (500), the photoelectric detection modules (500) are arranged at intervals along the centrifugal motion path of the water sample to be detected, the water sample to be detected stops when passing through one photoelectric detection module (500), and the photoelectric detection modules (500) emit light spots with different wavelengths into the water sample to be detected, so that element data in the water sample to be detected are automatically identified.

8. The microfluidic water quality detection method according to claim 1, wherein: in the sample placing process, the digestion bottle (200) and the disc chip (100) are placed in the same face of the detector in sequence, a rotatable cover door (700) is arranged on the surface of a shell (800) of the detector, the cover door (700) spans across the local upper surface and the front surface of the shell (800), an inner boss which is formed integrally and is close to the cover door (700) is arranged in the shell (800), and a placing opening for placing the digestion bottle (200) is formed in the inner boss.

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