CN116400032A - Microfluidic water quality detection method - Google Patents
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- CN116400032A CN116400032A CN202211610869.9A CN202211610869A CN116400032A CN 116400032 A CN116400032 A CN 116400032A CN 202211610869 A CN202211610869 A CN 202211610869A CN 116400032 A CN116400032 A CN 116400032A
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- 238000001514 detection method Methods 0.000 title claims abstract description 91
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 74
- 230000029087 digestion Effects 0.000 claims abstract description 85
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 69
- 238000000034 method Methods 0.000 claims abstract description 32
- 230000008569 process Effects 0.000 claims abstract description 28
- 238000006243 chemical reaction Methods 0.000 claims abstract description 23
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 238000010438 heat treatment Methods 0.000 claims abstract description 21
- 238000012546 transfer Methods 0.000 claims abstract description 21
- 238000004458 analytical method Methods 0.000 claims abstract description 8
- 230000007246 mechanism Effects 0.000 claims description 22
- 230000008859 change Effects 0.000 claims description 7
- 230000017525 heat dissipation Effects 0.000 claims description 7
- 230000009471 action Effects 0.000 claims description 5
- 238000011002 quantification Methods 0.000 claims description 3
- 230000017105 transposition Effects 0.000 claims description 2
- 238000012372 quality testing Methods 0.000 claims 1
- 230000001360 synchronised effect Effects 0.000 claims 1
- 239000002699 waste material Substances 0.000 abstract description 7
- 238000005070 sampling Methods 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 3
- 238000002360 preparation method Methods 0.000 abstract 1
- 238000012545 processing Methods 0.000 description 4
- 238000011161 development Methods 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- WABPQHHGFIMREM-UHFFFAOYSA-N lead(0) Chemical compound [Pb] WABPQHHGFIMREM-UHFFFAOYSA-N 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000009417 prefabrication Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A20/00—Water conservation; Efficient water supply; Efficient water use
- Y02A20/20—Controlling water pollution; Waste water treatment
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Abstract
The invention discloses a microfluidic water quality detection method, which specifically comprises the following steps of: placing a disk chip prefabricated with a detection water sample and a digestion bottle prefabricated with a sample adding reagent into a detector from different positions in sequence; heating and digestion: heating and digesting the sample adding reagent in the digestion bottle; transfer mixing: transferring the digested sample adding reagent to a tray chip, and mixing the digested sample adding reagent with a detection water sample prefabricated in the tray chip in advance; detection and analysis: sending out a detection light source to the mixed detection water sample, and analyzing data in the detection water sample; in the invention, the processes of sampling, high-temperature airtight digestion, quantitative, photoelectric detection and the like are all integrated on a fan-shaped chip with a size of a few centimeters through the microfluidic disc chip technology standardization, and the quantitative preparation of the reagent is completed, so that the complicated manual operation is avoided, the error is reduced, the data is accurate, the reaction reagent is micronized, the reagent and the sample liquid are less in demand, and the quantity of waste liquid can be greatly reduced compared with the conventional laboratory water quality detection.
Description
Technical Field
The invention relates to the technical field of water quality detection, in particular to a microfluidic water quality detection method.
Background
In the traditional water quality detection process, the water quality is generally detected by sampling on site and carrying the water quality into a laboratory, the detection reagent is also configured manually, then a proper amount of detection water sample is added according to detection requirements, quantitative detection of the reagent cannot be realized, the accuracy of detection data is not high, the control of the amount of waste liquid after detection is not facilitated, the operation of staff is also not facilitated by the mixing of multiple detection reagents, and the detection efficiency is reduced.
Disclosure of Invention
The invention mainly aims to provide a microfluidic water quality detection method, which aims to solve the existing technical problems.
In order to achieve the above purpose, the invention provides a microfluidic water quality detection method, which comprises the following steps,
sample placement: placing a disk chip prefabricated with a detection water sample and a digestion bottle prefabricated with a sample adding reagent into a detector from different positions in sequence;
heating and digestion: heating and digesting the sample adding reagent in the digestion bottle through a digestion mechanism;
transfer mixing: transferring the digested sample adding reagent to a tray chip, and mixing the digested sample adding reagent with a detection water sample prefabricated in the tray chip in advance;
detection and analysis: and (3) sending out a detection light source to the mixed detection water sample, and analyzing data in the detection water sample.
Further, in the transferring and mixing process, the digested sample adding reagent is transferred to the disc chip, the sample adding reagent is driven to be mixed with the detected water sample on the disc chip through centrifugal driving, the sample adding reagent is quantitatively mixed with the detected water sample through quantification before mixing, when the centrifugal rotating speed is small, the sample adding reagent enters the quantifying pond, and when the centrifugal rotating speed is gradually increased, the sample adding reagent gradually enters the detecting area, and the quantifying pond is communicated with the detecting area through a long and narrow channel.
Further, in the transfer process, the digested digestion bottle is rotated to the downward opening through the transfer mechanism, is inserted onto the disc chip in a vertical downward moving posture and stays on the disc chip, then the disc chip is driven to rotate for transposition, a liquid sac prefabricated on the disc chip is pierced through the piercing mechanism, a water sample and a sample adding reagent are detected to flow to be mixed with each other, and the mixing process is accelerated by matching with centrifugal action.
Further, the transfer mechanism comprises a plurality of transfer mechanisms,
the rotary component is used for driving the digestion bottle to change the posture after the digestion reaction is completed, so that the bottle mouth of the digestion bottle is vertically downward;
and the vertical moving part is used for driving the digestion bottle to move vertically, so that the digestion bottle after posture change moves downwards and is inserted into the disc chip.
Further, in the heating digestion process of the sample adding reagent in the digestion bottle, the rotation part is synchronously matched to drive the digestion bottle to rotate at a specified speed, so that the sample adding reagent therein fully undergoes digestion reaction, and then the sample adding reagent is cooled through the heat dissipation system, and then the subsequent reagent transfer is performed.
Further, in the digestion heating process, the bottom of the digestion bottle is attached to the heating structure, and different digestion reaction temperatures are controlled in real time according to different water samples to be detected through the temperature measuring element.
Further, in the detection analysis process, the water sample to be detected is detected sequentially through the photoelectric detection modules, the photoelectric detection modules are arranged at intervals along the centrifugal motion path of the water sample to be detected, the water sample to be detected stops when passing through one photoelectric detection module, the photoelectric detection modules emit light spots with different wavelengths into the water sample to be detected, and element data in the water sample to be detected are automatically identified.
Further, in the sample placing process, the digestion bottle and the tray chip are placed in sequence on the same side of the detector, a cover door capable of being opened in a rotating mode is arranged on the surface of the shell of the detector, the cover door stretches across the local upper surface and the front surface of the shell, an inner boss which is formed integrally and is close to the cover door is arranged in the shell, and a placing opening for placing the digestion bottle is formed in the inner boss.
The beneficial effects of the invention are as follows:
in the invention, the processes of sampling, high-temperature closed digestion, quantitative and stepwise reagent mixing, reaction and color development, photoelectric detection and the like are all integrated on a fan-shaped chip with a size of a few centimeters through the microfluidic disc chip technology in a standardized way, and the quantitative and full prefabrication of the reagent is completed, so that complicated manual operation is avoided, only a water sample is added to start detection, errors are reduced, data are accurate, the reaction reagent is micronized, the reagent and the sample liquid are less in required quantity, and compared with the conventional laboratory water quality detection, the waste liquid quantity can be greatly reduced.
Drawings
FIG. 1 is a schematic view of a digestion bottle and tray chip placement of the present invention;
FIG. 2 is a schematic illustration of the puncturing of the liquid sac 1 according to the present invention (puncturing of the liquid sac 1 in a 90 degree rotated state);
FIG. 3 is a schematic illustration of the puncturing of the liquid bladder 2 (puncturing of the liquid bladder 2 in a 90 rotated state) according to the present invention;
FIG. 4 is a schematic view showing the insertion of the digestion vessel 1 of the present invention (rotated 180 degrees to press into the digestion vessel 1);
FIG. 5 is a schematic view showing the insertion of the digestion vessel 2 according to the present invention (the digestion vessel 2 is pressed in a state rotated 180 degrees).
Reference numerals illustrate:
100. a disk chip; 200. digestion bottle; 300. a transfer mechanism; 310. a vertically moving member; 320. a rotating member; 400. a digestion mechanism; 500. a photoelectric detection module; 600. a lancing mechanism; 700. a cover door; 800. a housing.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the invention. Embodiments and features of embodiments in this application may be combined with each other without conflict. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It should be noted that, if directional indications (such as up, down, left, right, front, and rear … …) are included in the embodiments of the present invention, the directional indications are merely used to explain the relative positional relationship, movement conditions, etc. between the components in a specific posture (as shown in the drawings), and if the specific posture is changed, the directional indications are correspondingly changed.
In addition, if there is a description of "first", "second", etc. in the embodiments of the present invention, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In addition, the meaning of "and/or" as it appears throughout includes three parallel schemes, for example "A and/or B", including the A scheme, or the B scheme, or the scheme where A and B are satisfied simultaneously. In addition, "a plurality of" means two or more. In addition, the technical solutions of the embodiments may be combined with each other, but it is necessary that the combination of the technical solutions should be regarded as not existing when the combination of the technical solutions contradicts or cannot be realized on the basis of the realization of those skilled in the art.
Referring to fig. 1-5, the method for detecting water quality by micro-flow control of the present invention comprises the following steps,
sample placement: placing the tray chip 100 prefabricated with the detection water sample and the digestion bottle 200 prefabricated with the sample adding reagent into a detector from different positions in sequence;
heating and digestion: heating and digesting the sample adding reagent in the digestion bottle 200 through the digestion mechanism 400;
transfer mixing: transferring the digested sample adding reagent to the tray chip 100, and mixing the digested sample adding reagent with a detection water sample prefabricated in the tray chip 100 in advance;
detection and analysis: and (3) sending out a detection light source to the mixed detection water sample, and analyzing data in the detection water sample.
The invention is suitable for on-site water sample detection operation, and by mixing and detecting the prefabricated reagent and the detected water sample distribution, the operation of manually adding the detection reagent or the water sample is reduced, the detection accuracy is improved, and the detection efficiency is also improved.
The method integrates the processes of sampling, high-temperature airtight digestion, quantitative and stepwise reagent mixing, reaction and color development, photoelectric detection and the like on a fan-shaped chip with a size of a few centimeters (the specific structure is referred to a patent name submitted on the same day: a portable microfluidic water quality detector) through the microfluidic disk chip 100 technology standardization, the reagent quantification is completely prefabricated, the complicated manual operation is avoided, only a water sample is added for detection, the error is reduced, the data is accurate, the reaction reagent is micronized, the reagent and the sample liquid are less in demand, and the waste liquid amount can be greatly reduced compared with the conventional laboratory water quality detection.
In an embodiment, in the transferring and mixing process, the digested sample-adding reagent is transferred onto the tray chip 100, and is driven to be mixed with the detected water sample on the tray chip 100 by centrifugal driving, the sample-adding reagent is quantitatively mixed with the detected water sample before being mixed, when the centrifugal rotation speed is small, the sample-adding reagent enters the quantitative pool, and when the centrifugal rotation speed is gradually increased, the sample-adding reagent gradually enters the detection area, wherein the quantitative pool is communicated with the detection area through a long and narrow channel. In such an operation, quantitative addition of the reagent can be realized by controlling the rotation speed of centrifugation, so that an appropriate amount of reagent can be added according to different detection requirements, the detection accuracy is improved, and the reaction between the reagents is more sufficient by mixing multiple reagents step by step, so that the detection accuracy is further improved.
In an embodiment, in the transferring process, the digested digestion bottle 200 is inserted onto the tray chip 100 through the rotating component 320 of the transferring mechanism 300 until the bottle mouth is downward, and stays on the tray chip 100 in a vertically downward moving posture, then the tray chip 100 is driven to rotate and shift, the liquid sac prefabricated on the tray chip 100 is punctured by the puncturing mechanism 600, the water sample and the sample adding reagent are detected to flow to be mixed with each other, and the mixing process is accelerated in cooperation with the centrifugal action. In this way, the digestion bottle 200 is left on the tray chip 100, and excess waste liquid is left in the tray chip 100 by quantitative addition of reagents, so that the usual laboratory detection waste liquid is changed into solid chip waste, and is convenient to transport and process, and multiple paths of liquid are mixed step by the alternative operation of the puncturing mechanism 600 and the transferring mechanism 300, thereby facilitating the detection reaction.
Because of different detection requirements, different reagents and water samples to be detected need to be mixed, in the process of multi-path liquid mixing detection, multiple conversion and mixing processes need to be sequentially performed, please refer to fig. 2-5, different digestion bottles 200 are inserted onto different tray chips 100, and different liquid bags are sequentially punctured by a puncturing mechanism 600 described below, so that detection operations of different water samples to be detected are completed.
In one embodiment, the transfer mechanism 300 includes,
the rotating component 320 is used for driving the digestion bottle 200 to change the posture after the digestion reaction is completed, so that the bottle mouth of the digestion bottle 200 is vertically downward;
the vertical moving component 310 is configured to drive the digestion bottle 200 to move vertically, so that the digestion bottle 200 after posture conversion moves down and is inserted onto the tray chip 100.
After the digestion bottle 200 and the disc chip 100 are put into and the digestion reaction is completed, the rotation component 320 drives the digestion bottle 200 to change the posture, the digestion bottle is transferred onto the disc chip 100, reagent is transferred, the rotation component 320 is matched subsequently, the puncturing mechanism 600 is shifted in position, the liquid bags on the disc chip 100 are punctured, the mixing of multiple paths of liquid is realized, and the shifting mechanism 300 can change the positions of the digestion bottle 200 and the puncturing mechanism 600 alternately and is shifted.
In an embodiment, in the process of heating and digesting the sample adding reagent in the digestion bottle 200, the rotation component 320 is synchronously matched with the rotation component 320 to drive the digestion bottle 200 to rotate at a specified speed, so that the sample adding reagent therein fully undergoes digestion reaction, and then the sample adding reagent is cooled by a heat dissipation system, and then the subsequent reagent transfer is performed. In the digestion reaction process, the rotary component 320 drives the digestion bottle 200 to rotate, so that the digestion reaction rate of the sample adding reagent is accelerated, the overall detection efficiency is improved, and the temperature is reduced through the heat dissipation system, so that the temperature required by the subsequent reaction is quickly recovered, and the efficiency of the subsequent transfer mixing detection is further improved.
The heat dissipation system can adopt a heat dissipation pipe and a heat dissipation fan.
In an embodiment, during the digestion heating process, the bottom of the digestion bottle 200 is attached to the heating structure, and different digestion reaction temperatures are controlled in real time by the temperature measuring element according to different water samples to be detected. In such an operation, through contact conduction heating, the temperature required by digestion reaction can be met, and the action of the subsequent digestion bottle 200 during transfer can not be influenced, because the upper bottle body of the digestion bottle 200 is in a bare state at this time, other limiting structures are convenient to contact in the subsequent transfer process; the heating structure adopts a ceramic structure, can resist the high temperature of 350 ℃ at most, and the lead wire adopts the 0.5mm nickel wire, so that the heating structure has the advantages of quick heating and no power attenuation after long-term use.
In an embodiment, in the detection and analysis process, the water sample to be detected is sequentially detected by the photoelectric detection modules 500, the photoelectric detection modules 500 are arranged at intervals along the centrifugal motion path of the water sample to be detected, the water sample to be detected stops when passing through one photoelectric detection module 500, and the photoelectric detection modules 500 emit light spots with different wavelengths into the water sample to be detected, so that element data in the water sample to be detected can be automatically identified. The output end of the photoelectric detection module 500 is electrically connected with the data processing module, wherein the data processing module is electrically connected with the touch screen arranged on the surface of the box body and is used for displaying detected data. The photodetection module 500 emits light with a specific wavelength, and the light passes through the reacted liquid and is acquired by the receiving part, and can be converted into an electrical signal according to the acquired optical signal, and the corresponding value of the concentration is acquired through a corresponding analysis method. The invention patent CN113655011A of the company application, a micro-flow control detection system and a detection method thereof, discloses a corresponding photoelectric signal processing circuit.
In an embodiment, in the sample placing process, the digestion bottle 200 and the disk chip 100 are placed in sequence on the same surface of the detector, the surface of the shell 800 of the detector is provided with a cover door 700 which can be opened in a rotating way, the cover door 700 spans over the local upper surface and the front surface of the shell 800, an inner boss which is formed integrally and is close to the cover door 700 is arranged in the shell 800, and a placing opening for placing the digestion bottle 200 is formed in the inner boss. Because the action states of the digestion bottle 200 and the disc chip 100 in the detection process are different, the digestion bottle 200 needs to be moved vertically, and the disc chip 100 only needs to be rotated horizontally, and the cover door 700 crossing the adjacent surfaces is arranged on the surface of the shell 800, so that the digestion bottle 200 and the disc chip 100 can be taken conveniently at the same time, the shell 800 can be conveniently molded, the molding process is reduced, and the processing efficiency of the detector is improved.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.
Claims (8)
1. A microfluidic water quality detection method is characterized in that: in particular comprising the following steps of the method,
sample placement: placing a tray chip (100) prefabricated with a detection water sample and a digestion bottle (200) prefabricated with a sample adding reagent into a detector from different positions in sequence;
heating and digestion: heating and digesting the sample adding reagent in the digestion bottle (200) through a digestion mechanism (400);
transfer mixing: transferring the digested sample adding reagent to a tray chip (100), and mixing the digested sample adding reagent with a detection water sample prefabricated in the tray chip (100) in advance;
detection and analysis: and (3) sending out a detection light source to the mixed detection water sample, and analyzing data in the detection water sample.
2. The microfluidic water quality detection method according to claim 1, wherein: in the transfer mixing process, the digested sample adding reagent is transferred onto the tray chip (100), the sample adding reagent is driven to be mixed with a detected water sample on the tray chip (100) through centrifugal driving, the sample adding reagent is quantitatively mixed with the detected water sample through quantification before mixing, when the centrifugal rotating speed is small, the sample adding reagent enters the quantifying pond, and when the centrifugal rotating speed is gradually increased, the sample adding reagent gradually enters the detecting area, and the quantifying pond is communicated with the detecting area through a long and narrow channel.
3. The microfluidic water quality detection method according to claim 1, wherein: in the transfer process, the digested digestion bottle (200) is rotated to the bottle mouth downwards through the transfer mechanism (300), is inserted onto the tray chip (100) in a vertically downward moving posture and stays on the tray chip (100), then the tray chip (100) is driven to rotate for transposition, the liquid sac prefabricated on the tray chip (100) is punctured through the puncturing mechanism (600), the water sample and the sample adding reagent are detected to flow to be mixed with each other, and the mixing process is accelerated by matching with the centrifugal action.
4. A microfluidic water quality testing method according to claim 3, wherein: the transfer mechanism (300) comprises a plurality of transfer units,
the rotary component (320) is used for driving the digestion bottle (200) to change the posture after the digestion reaction is completed, so that the bottle mouth of the digestion bottle (200) is vertically downward;
and the vertical moving component (310) is used for driving the digestion bottle (200) to move vertically, so that the digestion bottle (200) subjected to posture change moves downwards and is inserted into the tray chip (100).
5. The microfluidic water quality detection method according to claim 4, wherein: in the heating digestion process of the sample adding reagent in the digestion bottle (200), the digestion bottle (200) is driven to rotate at a specified speed through synchronous cooperation of the rotating component (320) so that the sample adding reagent therein fully undergoes digestion reaction, and then the sample adding reagent is cooled through a heat dissipation system, and then subsequent reagent transfer is performed.
6. The microfluidic water quality detection method according to claim 1, wherein: in the digestion heating process, the bottom of the digestion bottle (200) is attached to the heating structure, and different digestion reaction temperatures are controlled in real time according to different water samples to be detected through the temperature measuring element.
7. The microfluidic water quality detection method according to claim 1, wherein: in the detection analysis process, the water sample to be detected is sequentially detected through the photoelectric detection modules (500), the photoelectric detection modules (500) are arranged at intervals along the centrifugal motion path of the water sample to be detected, the water sample to be detected stops when passing through one photoelectric detection module (500), and the photoelectric detection modules (500) emit light spots with different wavelengths into the water sample to be detected, so that element data in the water sample to be detected are automatically identified.
8. The microfluidic water quality detection method according to claim 1, wherein: in the sample placing process, the digestion bottle (200) and the disc chip (100) are placed in the same face of the detector in sequence, a rotatable cover door (700) is arranged on the surface of a shell (800) of the detector, the cover door (700) spans across the local upper surface and the front surface of the shell (800), an inner boss which is formed integrally and is close to the cover door (700) is arranged in the shell (800), and a placing opening for placing the digestion bottle (200) is formed in the inner boss.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN117405914A (en) * | 2023-09-21 | 2024-01-16 | 湖北微流控科技有限公司 | Quantitative transfer method and system for centrifugal microfluidic reagent |
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