CN117402709B - Microorganism culture monitoring device and method - Google Patents

Abstract

The invention relates to the technical field of microorganism culture monitoring, in particular to a microorganism culture monitoring device and a method, wherein in a sampling mechanism designed by the invention, under the condition that a sealing plug is not opened, a sampling rod can be mutually matched with a sampling groove through an opening plate capable of moving left and right, so that a valve can be opened, culture solution outside a stirring rod enters the inside of the sampling groove after passing through a liquid inlet groove, further, the culture solution in a culture tube can be subjected to sampling treatment under the condition that the sealing plug is not opened, the possibility that external mixed bacteria enter the culture tube is reduced, and meanwhile, a plurality of liquid inlet grooves with different heights can be used for carrying out sampling treatment on culture solutions with different heights in the culture tube, so that the monitoring treatment on the culture solutions with different heights in the follow-up monitoring process is ensured, and the monitoring result is more representative.

Description

Microorganism culture monitoring device and method

Technical Field

The application relates to the technical field of microorganism culture monitoring, in particular to a microorganism culture monitoring device and a method.

Background

The microbial cultivation refers to the rapid growth and propagation of certain microorganisms by means of a manually prepared culture medium and artificially created culture conditions (such as culture temperature and the like), and is called as microbial cultivation, and in the microbial cultivation process, the growth of the microorganisms in the culture solution is often required to be monitored in order to determine the quantity of the microorganisms in the culture solution.

The device and the method for monitoring the cell culture box disclosed by the publication number CN111676134A relate to the technical field of cell culture monitoring: the incubator comprises a base and an incubator assembly, wherein the incubator assembly is arranged on the top of the base; the rotating assembly is arranged at the bottom of the incubator assembly, the rotating part of the rotating assembly is inserted into the inner cavity of the incubator assembly, and the driving part of the rotating assembly is arranged in the top groove of the base; the plurality of light-transmitting rack assemblies are uniformly arranged on the outer wall of the rotating part of the rotating assembly, and the light-transmitting rack assemblies are arranged in the inner cavity of the incubator assembly; the mobile monitoring assembly is arranged on the right side of the top of the incubator assembly, and can track the cell culture time, environment and change, so that the purpose of observing and controlling cells in real time is achieved.

However, the prior art described above still has some drawbacks when monitoring for cell culture: the device monitors cells inside the culture assembly by opening the box door in the cell monitoring process, and when the box door is opened, external mixed bacteria possibly enter the inside of the culture box assembly, so that mixed bacteria are doped in the culture solution inside the culture box assembly, thereby causing pollution of the culture solution, affecting growth of the cells and even causing death of the cells.

Based on this, in the statement of the above point of view, the prior art still has room for improvement in the monitoring of microbial cultures.

Disclosure of Invention

In order to solve the technical problems, the application provides a microorganism culture monitoring device and a method, which adopt the following technical scheme:

in a first aspect, a microorganism cultivates monitoring devices, including the horizontal plate that can reciprocate from top to bottom rocks, horizontal plate up end left and right sides all runs through the rotation and installs the ascending cultivate pipe of upper and lower extension and opening, cultivate the pipe up end install the sealing plug, the sealing plug up end runs through the rotation and installs and be used for playing stirring effect and turn to opposite puddler with cultivate the pipe to cultivate the intraductal culture solution of cultivateing, installs sampling mechanism on the puddler.

The sampling mechanism comprises a communicating groove which is formed in the stirring rod and penetrates up and down, a plurality of liquid inlets which are uniformly distributed from top to bottom and are communicated with the communicating groove are formed in the right side wall of the stirring rod, a valve capable of automatically resetting is arranged in the liquid inlets, a type frame with a downward opening is arranged on the upper end face of the stirring rod and above the communicating groove, a type frame horizontal section penetrates through and is provided with a sampling rod which extends up and down and is matched with the communicating groove, scale marks corresponding to the liquid inlets one to one are arranged on the surface of the sampling rod, a sampling groove which is of an L-shaped structure and is used for storing culture liquid is formed in the inner side wall of the sampling rod, and a switching plate which can reciprocate and is matched with the valve is arranged on the right end face of the horizontal section of the sampling groove.

Preferably, the horizontal plate below is provided with the horizontal stand, horizontal stand up end mid-mounting has driving motor, driving motor output up end installs the drive shaft of variable length, driving motor's one end and horizontal plate fixed connection are kept away from to the drive shaft, vertical pole is installed to horizontal stand up end and lie in driving motor left and right sides, two vertical pole up ends and lie in the horizontal plate below and install the annular frame jointly, a plurality of arc archs are evenly installed to annular frame up end circumference, the horizontal plate lower terminal surface is installed bilateral symmetry and is rocked even piece with arc protruding matched with.

Preferably, the driving shaft comprises a first shaft and a second shaft which are distributed from top to bottom, wherein the lower end of the first shaft is arranged on the upper end surface of the second shaft in a limiting sliding manner, the first shaft is provided with an annular bulge, and the first shaft is sleeved with a reset spring fixedly connected with the annular bulge and the upper end of the second shaft.

Preferably, the annular rack is arranged in the middle of the upper end surface of the horizontal rack, a supporting rod corresponding to the culture tube is slidably arranged on the upper end surface of the annular rack, a connecting rod which slides up and down and is matched with the supporting rod on the same side is limited on the upper end surface of the supporting rod, one end of the connecting rod, which is far away from the supporting rod on the same side, is fixedly connected with the lower end of the culture tube on the same side, the annular rack plate is arranged on the upper end surface of the horizontal rack through a left-right symmetrical fixing protrusion, and a first gear meshed with the annular rack plate is arranged on the supporting rod.

Preferably, the middle part of the upper end surface of the horizontal plate is provided with a fixed shaft, the fixed shaft is connected with the left culture tube in a belt transmission mode, a gear II is arranged above the fixed shaft, and two stirring rods are respectively provided with a gear III meshed with the gear I.

Preferably, a plurality of stirring rods which are uniformly distributed from top to bottom and are obliquely arranged are arranged on the left side wall of the stirring rod, an input groove which extends up and down and is provided with an upward opening is formed in the stirring rod, an output groove which is communicated with the input groove is formed in the stirring rod, a linkage rod is arranged in the output groove, a plurality of liquid outlet grooves which are distributed along the extending direction of the stirring rod are uniformly formed in the output groove, rubber plugs which are in one-to-one correspondence with the liquid outlet grooves and play a sealing role on the liquid outlet grooves are arranged on the linkage rod, a driving rod which moves up and down is arranged in the input groove in a sliding mode, and the linkage rod is hinged between the driving rod and the corresponding linkage rod.

Preferably, a connecting spring rod for resetting the opening plate is mounted between the opening plate and the vertical section of the sampling groove, a pushing block matched with the valve is arranged on the right end face of the opening plate, an annular gasket matched with the opening plate is mounted on the horizontal section of the sampling groove, a yielding groove is formed in the upper end face of the horizontal section of the sampling groove and above the opening plate, a linkage protrusion fixedly connected with the opening plate is arranged in the yielding groove in a limiting sliding manner, an air inlet groove which is penetrated with the sampling rod and is of an L-shaped structure is formed in the left side of the yielding groove, and a piston fixedly connected with the linkage protrusion is arranged in the horizontal section of the air inlet groove in a sliding manner.

In a second aspect, a method for monitoring the culture of microorganisms comprises the steps of: s1: and (3) feeding treatment: and taking down the sealing plug, pouring the microbial liquid and the nutrient solution to be cultured into the culture tube to obtain the culture solution, and resetting the sealing plug.

S2: stirring: the stirring rod is rotated to stir and mix the culture solution, and then the culture tube is placed still.

S3: sampling: the culture solution in the culture tube is sampled and monitored by a sampling mechanism.

In summary, the present application includes at least one of the following beneficial technical effects: 1. according to the sampling mechanism designed by the invention, under the condition that the sealing plug is not opened, the sampling rod is matched with the sampling groove through the opening plate capable of moving left and right, so that the valve can be opened, the culture solution outside the stirring rod enters the sampling groove after passing through the liquid inlet groove, the culture solution in the culture tube can be sampled and treated under the condition that the sealing plug is not opened, the possibility that external mixed bacteria enter the culture tube is reduced, meanwhile, a plurality of liquid inlet grooves with different heights can be used for sampling and treating the culture solutions with different heights in the culture tube, the monitoring treatment of the culture solutions with different heights in the subsequent monitoring process is ensured, and the monitoring result is more representative.

2. The rotating horizontal plate designed by the invention can be mutually matched with the arc-shaped protrusions through the shaking connecting blocks to carry out shaking treatment on the culture bottle, so that the microorganism liquid and the nutrient solution in the culture bottle are ensured to be uniformly mixed, the direction of the microorganism liquid and the nutrient solution in the culture bottle is the same as that of the stirring rod in the rotation process of the culture bottle, the uniformity of the culture solution in the stirring process is further improved, and the growth effect of microorganisms is improved.

3. When needs are to pour nutrient solution into the inside of cultivateing the pipe, pour into the culture solution into the output inslot portion through the input groove, open and shut and then can pour into the nutrient solution into the inside of cultivateing the pipe under the condition that does not open the sealing plug through the play liquid groove on the control stirring connecting rod, reduce the possibility that miscellaneous fungus got into the cultivateing pipe, many stirring connecting rods mutually support simultaneously can make the nutrient solution go down to cultivate inside different height of pipe, further improves the homogeneity of nutrient solution in the culture solution.

Drawings

Fig. 1 is a schematic perspective view of the present invention.

FIG. 2 is a schematic view of the upper part of the present invention in a partially isolated configuration.

Fig. 3 is an enlarged view of a portion of fig. 2 at a in accordance with the present invention.

FIG. 4 is a schematic view of the lower part of the present invention in a partially isolated configuration.

Fig. 5 is an enlarged view of a portion of fig. 4 at B in accordance with the present invention.

FIG. 6 is a schematic view showing the internal structure of the culture tube of the present invention.

Fig. 7 is an enlarged view of a portion of fig. 6 at C in accordance with the present invention.

Fig. 8 is a partial enlarged view of the invention at D in fig. 6.

FIG. 9 is a schematic view showing the internal perspective structure of the culture tube according to the present invention.

Fig. 10 is an enlarged view of a portion of fig. 9 at E in accordance with the present invention.

FIG. 11 is a flow chart of a method for monitoring the culture of microorganisms according to the present invention.

Reference numerals illustrate: 1. a horizontal plate; 11. a horizontal frame; 111. an annular frame; 112. a support rod; 113. a connecting rod; 114. annular rack plate; 115. a first gear; 116. a fixed shaft; 117. a second gear; 118. a third gear; 12. a driving motor; 13. a drive shaft; 131. a first shaft; 132. a second shaft; 133. an annular protrusion; 134. a return spring; 14. a vertical rod; 15. an annular frame; 16. arc-shaped bulges; 17. shaking the connecting blocks; 2. a culture tube; 3. a sealing plug; 4. a stirring rod; 41. a stirring connecting rod; 42. an input slot; 43. an output slot; 44. a linkage rod; 45. a liquid outlet groove; 46. a rubber stopper; 47. a driving rod; 48. a linkage connecting rod; 5. a sampling mechanism; 51. a communication groove; 52. a liquid inlet tank; 53. a valve; 54. type rack; 55. a sampling rod; 56. a sampling groove; 561. connecting a spring rod; 562. a pushing block; 563. an annular gasket; 564. a relief groove; 565. a linkage protrusion; 566. an air inlet groove; 567. a piston; 568. a liquid leakage groove; 569. a sealing block; 57. an opening plate; 58. graduation marks.

Detailed Description

The present application is described in further detail below in conjunction with fig. 1-11.

The embodiment of the application discloses a microorganism culture monitoring device and a method, wherein the method of not opening the device is used for sampling the culture solution in the device and filling the culture solution for treatment, so that the possibility that external mixed bacteria enter the device is reduced.

Embodiment one: referring to fig. 1, a microorganism culture monitoring device, including horizontal plate 1 that can reciprocate from top to bottom rocks, horizontal plate 1 up end left and right sides all runs through and rotates and install the ascending cultivate pipe 2 of upper and lower extension and opening, cultivate pipe 2 up end install sealing plug 3, sealing plug 3 up end runs through and rotates and install and be used for playing stirring effect and with cultivate pipe 2 reverse puddler 4 of turning to cultivate the inside culture solution of pipe 2, install sampling mechanism 5 on the puddler 4.

The sealing plug 3 is taken down, microorganism liquid and nutrient solution to be cultured are poured into the culture tube 2 to obtain culture solution, the sealing plug 3 is reset, the culture solution can be stirred by rotating the stirring rod 4 to be uniformly mixed, and when the culture solution is required to be taken out for monitoring, the culture solution is taken out from the culture tube 2 for monitoring through the sampling mechanism 5.

With continued reference to fig. 1, in order to ensure uniformity of mixing of culture solution in the culture tube 2, a horizontal frame 11 is provided below the horizontal frame 1, a driving motor 12 is installed in the middle of an upper end surface of the horizontal frame 11, a driving shaft 13 with a variable length is installed on an upper end surface of an output end of the driving motor 12, one end of the driving shaft 13 away from the driving motor 12 is fixedly connected with the horizontal frame 1, the driving motor 12 and the driving shaft 13 are mutually matched to drive the horizontal frame 1 to rotate, vertical rods 14 are installed on the upper end surface of the horizontal frame 11 and on the left side and the right side of the driving motor 12, an annular frame 15 is jointly installed on the upper end surface of the two vertical rods 14 and below the horizontal frame 1, a plurality of arc-shaped protrusions 16 are uniformly installed on the upper end surface of the annular frame 15, shaking connecting blocks 17 which are symmetrical left and right and are matched with the arc-shaped protrusions 16 are installed on the lower end surface of the horizontal frame 1, and the horizontal frame 1 can shake up and down through the mutual matching of the shaking connecting blocks 17 and the arc-shaped protrusions 16 in the rotation process.

Starting driving motor 12, driving motor 12 output shaft rotates the in-process and drives drive shaft 13 to rotate, and drive shaft 13 rotates the in-process and drives cultivate tub 2 round drive shaft 13 circumference through horizontal plate 1, and horizontal plate 1 rotates the in-process and can drive cultivate tub 2 and rock from top to bottom through rocking link 17 and arc protruding 16 mutually supporting, cultivate tub 2 and rock the in-process from top to bottom and shake the inside culture solution and shake even processing, guarantees the inside microorganism liquid of culture solution and nutrient solution mixed homogeneity.

Referring to fig. 2, the driving shaft 13 is of variable length, and the driving shaft 13 according to the present invention is composed of a first shaft 131 and a second shaft 132 distributed from top to bottom, wherein the lower end of the first shaft 131 is slidably disposed on the upper end surface of the second shaft 132, the first shaft 131 is provided with an annular protrusion 133, and the first shaft 131 is sleeved with a return spring 134 fixedly connected to the annular protrusion 133 and the upper end of the second shaft 132.

The first shaft 131 slides up and down in the reciprocating manner on the second shaft 132 in the vertical shaking process of the horizontal plate 1, so that the second shaft 132 and the first shaft 131 are matched with each other to play a yielding role in the vertical reciprocating shaking process of the horizontal plate 1, the driving shaft 13 is prevented from being broken rigidly, the annular bulge 133 and the reset spring 134 are matched with each other to play a damping role on the second shaft 132, rigid impact between the first shaft 131 and the second shaft 132 is avoided, and the first shaft 131 and the second shaft 132 are protected.

Referring to fig. 4 to 5, in order to further ensure uniformity of mixing of the culture solution in the culture tube 2, an annular rack 111 is mounted in the middle of an upper end surface of the horizontal rack 11, a support rod 112 corresponding to the culture tube 2 is slidably disposed on the upper end surface of the annular rack 111, a connecting rod 113 which slides up and down and is matched with the support rod 112 on the same side is disposed on the upper end surface of the support rod 112 in a limited manner, synchronous rotation of the two can be ensured by the connecting rod 113 and the support rod 112 in a limited sliding manner, one end of the connecting rod 113, which is far away from the support rod 112 on the same side, is fixedly connected with the lower end of the culture tube 2 on the same side, an annular rack 114 is mounted on the upper end surface of the horizontal rack 11 through a left-right symmetrical fixing protrusion, a first gear 115 meshed with the annular rack 114 is mounted on the support rod 112, and the support rod 112 can be driven to rotate by the first gear 115 and the annular rack 114 in a circumferential rotation process.

Referring back to fig. 2, a fixed shaft 116 is mounted in the middle of the upper end surface of the horizontal plate 1, the fixed shaft 116 is connected with the left culture tube 2 in a belt transmission mode, a second gear 117 is mounted above the fixed shaft 116, a third gear 118 meshed with the first gear 115 is mounted on the two stirring rods 4, and the stirring rods 4 are mutually matched and rotated through the third gear 118 and the second gear 117 in the rotation process.

The first gear 115 and the annular rack plate 114 are mutually matched to drive the support rod 112 to rotate in the circumferential rotation process of the two culture tubes 2 around the driving shaft 13, the support rod 112 drives the culture tubes 2 to rotate through the connecting rod 113 in the rotation process of the support rod 112, the limiting sliding arrangement of the connecting rod 113 and the support rod 112 can play a yielding role in the up-and-down reciprocating shaking process of the culture tubes 2, the stirring rod 4 is driven to rotate through the third gear 118, the fixing shaft 116 and the second gear 117 in the rotation process of the culture tubes 2, the stirring rod 4 can stir the culture fluid in the rotation process of the stirring rod 4, the uniformity of culture fluid mixing is further guaranteed, and the microorganisms in different positions can obtain enough nutrition in the growth process.

The first gear 115 and the annular rack plate 114, and the third gear 118 and the second gear 117 are mutually matched to still respectively drive the culture tube 2 and the stirring rod 4 to rotate under the condition of reducing external driving, so that energy sources are effectively saved, and meanwhile, the stirring rod 4 and the culture tube 2 are opposite in direction of rotation, so that the culture solution receives different shaking force directions, and the uniformity of the culture solution in the stirring process is further improved.

Referring to fig. 6, 7 and 9, in order to avoid exposing the internal environment of the culture tube 2 to the outside of the air, the sampling mechanism 5 of the present invention includes a communicating groove 51 formed inside the stirring rod 4 and penetrating up and down, a plurality of liquid inlets 52 uniformly distributed from top to bottom and penetrating through the communicating groove 51 are formed on the right side wall of the stirring rod 4, an automatically resettable valve 53 is mounted inside the liquid inlets 52, the valve 53 can control the opening and closing of the liquid inlets 52, a type frame 54 with a downward opening is mounted on the upper end surface of the stirring rod 4 and above the communicating groove 51, a sampling rod 55 extending up and down and matching the communicating groove 51 is mounted on the horizontal section of the type frame 54, the sampling rod 55 can sample the culture liquid, a scale mark 58 (fig. 3) corresponding to the liquid inlets 52 one by one is provided on the surface of the sampling rod 55, the relative positions of the scale mark 58 and the type frame 54 can determine the specific positions of the lower ends of the sampling rod 55, an L-shaped structure is mounted inside the sampling rod 56 for storing the culture liquid, a sampling plate 57 is mounted on the right end surface of the sampling rod 56, and the horizontal section of the sampling rod 56 is reciprocally movable and the right end surface is provided with a sampling plate 57 capable of controlling the opening and closing of the sampling plate 57.

Referring to fig. 7, a connection spring lever 561 for resetting the opening and closing plate 57 is mounted between the opening and closing plate 57 and the vertical section of the sampling slot 56, a pushing block 562 matched with the valve 53 is disposed on the right end surface of the opening and closing plate 57, the pushing block 562 can be used for pushing the opening and closing of the valve 53, an annular gasket 563 matched with the opening and closing plate 57 is mounted on the horizontal section of the sampling slot 56, a yielding slot 564 is disposed on the upper end surface of the horizontal section of the sampling slot 56 and above the opening and closing plate 57, a linkage protrusion 565 fixedly connected with the opening and closing plate 57 is disposed in the inner limit sliding of the yielding slot 564, the movement of the opening and closing plate 57 can be controlled by the linkage protrusion 565, an air inlet slot 566 which is penetrated with the opening and has an L-shaped structure is disposed in the left side of the opening and closing plate 57, a piston 567 fixedly connected with the linkage protrusion 565 is slidingly disposed on the horizontal section of the air inlet slot 566, and the piston 567 is used for controlling the movement of the linkage protrusion 565.

The lower end of the sampling tank 56 is provided with a drain tank 568, the opening and closing plate 57 is provided with a sealing block 569 for sealing the drain tank 568, the drain tank 568 is opened at the initial position, an air outlet of the external air pump is connected with the air inlet tank 566 by a rubber hose (both the external air pump and the rubber hose are not shown), when the sampling is needed, the driving motor 12 is closed, the sampling rod 55 is pushed to move downwards according to the height of the needed sampling at the moment, the sampling rod 55 is enabled to move to a designated scale line 58, the scale line 58 is enabled to be level with the horizontal section of the type frame 54, at the moment, the horizontal section of the sampling tank 56 is communicated with the corresponding liquid inlet tank 52, the external air pump is started to pump air into the air inlet tank 566, the air is driven to move to the left side of the piston 567, the piston 567 is driven to move rightwards in the right in the process of the piston 567 by the linkage protrusion 565, at the moment, the opening plate 57 is opened by pushing the valve 53 in the right-pushing process of the opening plate 57, the spring rod is pulled to move rightwards according to the height of the needed sampling, the sampling rod 55 is enabled to move to the designated scale line 58, the scale line is enabled to be enabled to move horizontally, the scale line 58 is enabled to be leveled with the scale line 54, the corresponding scale line is enabled to be leveled with the corresponding to the corresponding scale line 52, the corresponding to be opened and the horizontal section of the liquid inlet tank 53 is opened, the horizontal section of the liquid inlet tank 56 is opened, and the horizontal section is opened to be opened, and the horizontal section is opened, and the corresponding to the horizontal section is opened, and the valve section 53 is opened.

When the sampling is finished, the external air pump is deflated, the spring rod 561 is connected at the moment to drive the opening and closing plate 57 to reset, the piston 567 is synchronously driven to reset through the linkage protrusions 565 in the resetting process of the sampling groove 56 closing the opening and closing plate 57, the pushing block 562 is synchronously driven to reset in the resetting process of the opening and closing plate 57, the valve 53 is synchronous at the moment, the liquid inlet groove 52 is closed, the sampling is finished, and the sampled sample is monitored.

When the sealing block 569 is synchronously reset after the opening and closing plate 57 is reset, the drain tank 568 is opened, and the culture solution on the right side of the opening and closing plate 57 in the horizontal section of the sampling tank 56 leaks downwards through the drain tank 568 and falls to the bottom of the communicating tank 51, so that the possibility of waste of the culture solution caused by overflow of the culture solution outside the sampling tank 56 after the sampling rod 55 is taken out is avoided.

Embodiment two: referring to fig. 7 to 10, on the basis of the first embodiment, along with the growth of microorganisms, there may be insufficient nutrient components in the culture solution, so in order to timely fill nutrition into the culture solution, a plurality of stirring rods 41 uniformly distributed from top to bottom and obliquely arranged are installed on the left side wall of the stirring rod 4, an input groove 42 extending up and down and having an upward opening is provided in the stirring rod 4, the input groove 42 can be used for storing the nutrient solution, an output groove 43 communicated with the input groove 42 is provided in the stirring rod 41, a linkage rod 44 is provided in the output groove 43, a plurality of liquid outlet grooves 45 distributed along the extending direction of the stirring rod 41 are uniformly provided in the output groove 43, the output groove 43 and the liquid outlet grooves 45 are mutually matched to carry out conveying treatment on the nutrient solution, rubber plugs 46 which are in one-to-one correspondence with the liquid outlet grooves 45 and play a sealing role are provided on the linkage rod 44, a driving rod 47 moving up and down is slidably provided in the input groove 42, a linkage rod 48 is jointly hinged between the driving rod 47 and the corresponding rod 44, and the linkage rod 47 can be mutually matched with the linkage rod 45 through the linkage rod 48.

During specific work, the culture solution is poured into the input groove 42 in the initial state, after microorganisms grow for a period of time, nutrient solution needs to be poured into the culture solution, at the moment, the driving rod 47 is pressed downwards through external force and air is pumped into the stirring rod 4 through external force, the driving rod 47 moves downwards, the linkage rod 44 is driven to move along the stirring rod 41 through the linkage rod 48 in the process of moving the driving rod 44, the rubber plug 46 is driven to be opened in the process of moving the linkage rod 44, at the moment, the nutrient solution is extruded from the output groove 43 through the liquid outlet groove 45 through air, and is output into the culture solution, further, the nutrient solution can be poured into the culture tube 2 under the condition that the sealing plug 3 is not opened through controlling the liquid outlet groove 45 on the stirring rod 41, the possibility that mixed bacteria enter the culture tube 2 is reduced, and meanwhile, the plurality of stirring rods 41 are mutually matched to enable the nutrient solution to fall into different heights inside the culture tube 2, and the uniformity of the nutrient solution in the culture solution is further improved.

After the nutrient solution is filled, the driving rod 47 is reset, the rubber plug 46 is synchronously reset, the liquid outlet groove 45 is sealed at the moment, and the microorganism can be cultivated and monitored by repeating the actions.

Referring to fig. 11, the invention further provides a method for monitoring the culture of microorganisms, comprising the following steps: s1: and (3) feeding treatment: and taking down the sealing plug 3, pouring the microbial liquid and the nutrient solution to be cultured into the culture tube 2 to obtain the culture solution, and resetting the sealing plug 3.

S2: stirring: the stirring rod 4 is rotated to stir and mix the culture solution, and then the culture tube 2 is left to stand.

S3: sampling and monitoring: the culture liquid in the culture tube 2 is subjected to sampling monitoring treatment by the sampling mechanism 5.

The embodiments of the present invention are all preferred embodiments of the present invention, and are not intended to limit the scope of the present invention in this way, therefore: all equivalent changes in structure, shape and principle of the invention should be covered in the scope of protection of the invention.

Claims (5)

1. The utility model provides a monitoring devices is cultivateed to microorganism, includes horizontal plate (1) that can reciprocate from top to bottom, and horizontal plate (1) up end left and right sides all runs through rotation and installs extension from top to bottom and ascending cultivate pipe (2) of opening, its characterized in that: the utility model provides a cultivate pipe (2) up end install sealing plug (3), sealing plug (3) up end runs through and rotates and install puddler (4) that are used for playing stirring effect and reverse with cultivate pipe (2) direction of rotation to cultivate inside culture solution of pipe (2), install sampling mechanism (5) on puddler (4), wherein:

the sampling mechanism (5) comprises a communication groove (51) which is formed in the stirring rod (4) and is communicated up and down, a plurality of liquid inlet grooves (52) which are uniformly distributed from top to bottom and are communicated with the communication groove (51) are formed in the right side wall of the stirring rod (4), a valve (53) capable of automatically resetting is arranged in the liquid inlet grooves (52), a type frame (54) with a downward opening is arranged on the upper end face of the stirring rod (4) and above the communication groove (51), a sampling rod (55) which extends up and down and is matched with the communication groove (51) is arranged at the horizontal section of the type frame (54) in a penetrating manner, scale marks (58) which are in one-to-one correspondence with the liquid inlet grooves (52) are formed in the surface of the sampling rod (55), an L-shaped structure and are used for storing culture liquid are formed in the sampling rod (55), and an opening and closing plate (57) which can reciprocate and is matched with the valve (53) is arranged on the right end face of the horizontal section of the sampling rod (56).

The horizontal plate (1) is provided with a horizontal frame (11) below, a driving motor (12) is arranged in the middle of the upper end face of the horizontal frame (11), a driving shaft (13) with variable length is arranged on the upper end face of the output end of the driving motor (12), one end of the driving shaft (13) away from the driving motor (12) is fixedly connected with the horizontal plate (1), vertical rods (14) are arranged on the left side and the right side of the upper end face of the horizontal frame (11) and positioned on the left side and the right side of the driving motor (12), annular frames (15) are jointly arranged on the upper end faces of the two vertical rods (14) and positioned below the horizontal plate (1), a plurality of arc-shaped bulges (16) are uniformly arranged on the circumferential direction of the upper end faces of the annular frames (15), and shaking connecting blocks (17) which are symmetrical left and right and matched with the arc-shaped bulges (16) are arranged on the lower end faces of the horizontal plate (1);

the driving shaft (13) consists of a first shaft (131) and a second shaft (132) which are distributed from top to bottom, wherein the lower end of the first shaft (131) is arranged on the upper end surface of the second shaft (132) in a limiting sliding manner, the first shaft (131) is provided with an annular bulge (133), the first shaft (131) is sleeved with the annular bulge (133), and the upper end of the second shaft (132) is fixedly connected with a reset spring (134);

the horizontal rack is characterized in that an annular rack (111) is arranged in the middle of the upper end face of the horizontal rack (11), a supporting rod (112) corresponding to the culture tube (2) is arranged on the upper end face of the annular rack (111) in a sliding mode, a connecting rod (113) which slides up and down and is matched with the supporting rod (112) on the same side is arranged on the upper end face of the supporting rod (112) in a limiting mode, one end, far away from the supporting rod (112) on the same side, of the connecting rod (113) is fixedly connected with the lower end of the culture tube (2) on the same side, annular rack plates (114) are arranged on the upper end face of the horizontal rack (11) through left-right symmetrical fixing protrusions, and a first gear (115) meshed with the annular rack plates (114) is arranged on the supporting rod (112).

2. The microbial cultivation monitoring device of claim 1, wherein: the utility model discloses a device for detecting the position of the valve, including opening and closing plate (57) and sampling groove (56), install jointly between the vertical section of opening and closing plate (57) and sampling groove (56) and be used for carrying out connection spring bar (561) that resets to opening and closing plate (57), opening and closing plate (57) right-hand member face is provided with and valve (53) matched with impeller (562), install on sampling groove (56) horizontal segment with opening and closing plate (57) matched with annular packing ring (563), sampling groove (56) horizontal segment up end just is located opening and closing plate (57) top and offered and step down groove (564), step down groove (564) inside spacing slip be provided with opening and closing plate (57) fixed connection's linkage arch (565), sampling rod (55) inside just be located step down groove (564) left side and offered and be mutually and be L type structure's air inlet groove (566), air inlet groove (566) horizontal segment slip is provided with piston (567) with linkage arch (565) fixed connection.

3. The microbial cultivation monitoring device of claim 1, wherein: the middle part of the upper end surface of the horizontal plate (1) is provided with a fixed shaft (116), the fixed shaft (116) is connected with the left culture tube (2) in a belt transmission mode, a gear II (117) is arranged above the fixed shaft (116), and two stirring rods (4) are respectively provided with a gear III (118) meshed with the gear I (115).

4. The microbial cultivation monitoring device of claim 1, wherein: the stirring rod (4) left side wall install a plurality of stirring connecting rods (41) that evenly distributed and slope set up from top to bottom, input groove (42) that extend from top to bottom and open upwards are offered to stirring rod (4) inside, output groove (43) that link up with input groove (42) are offered to stirring rod (41) inside, output groove (43) inside is provided with gangbar (44), evenly offered a plurality of play liquid groove (45) that distribute along stirring rod (41) extending direction on output groove (43), be provided with on gangbar (44) with play liquid groove (45) one-to-one and play rubber stopper (46) of sealed effect thereof, inside slip of input groove (42) is provided with actuating lever (47) that reciprocates, common articulated between actuating lever (47) and the gangbar (44) that correspond has gangbar (48).

5. A method for monitoring the culture of microorganisms comprising using the microorganism culture monitoring device according to any one of claims 1 to 4, characterized in that: the application method comprises the following steps:

s1: and (3) feeding treatment: taking down the sealing plug (3), pouring the microbial fluid and nutrient solution to be cultured into the culture tube (2) to obtain a culture solution, and resetting the sealing plug (3);

s2: stirring: rotating the stirring rod (4) to stir and mix the culture solution, and then standing the culture tube (2);

s3: sampling and monitoring: the culture solution in the culture tube (2) is sampled and monitored by a sampling mechanism (5).