CN1173997C - Process for extracting high-purity kanjak glucomannosan - Google Patents

Abstract

The present invention provides a technology for preparing high purity konjak glucomannan from refined konjak powder. The technology comprises: refined konjak powder is used as raw materials, various impurities in the refined powder are effectively removed by methods, such as physical separation, enzymolysis, mixed solvent extraction, pure water purification, dehydration, etc., and high purity glucomannan is obtained. The molecular chains of glucomannan are not destroyed, and the high viscosity and the medicinal characteristics of the glucomannan are maintained.

Description

The extraction process of high-purity kanjak glucomannosan

The present invention relates to a kind of processing method of from konjaku powder, extracting the high purity konjac glucomanna.

Except that containing konjac glucomanna, also contain other material of 20～35% in the konjaku powder, these materials are mainly: starch, fat, protein, soluble sugar, Mierocrystalline cellulose, ash content, sulfurous gas, muriate, pathogenic bacterium etc.

At present, from konjaku powder, extract konjac glucomanna and generally adopt ethanol precipitation.Mantoquita method, vacuum freeze-drying method or adopt with amylase, saccharifying enzyme and handle, the technology of ethanol sedimentation, more than these methods cut both ways, but all can not promptly can not get highly purified konjac glucomanna to greatest extent with the contaminant removal in the konjaku powder.

For this reason, the objective of the invention is to overcome the deficiency of prior art, and a kind of processing method that can extract the high purity konjac glucomanna from konjaku powder is provided.

The technical process of extracting the high purity konjac glucomanna from konjaku powder that realizes the object of the invention is as follows:

(1) konjaku powder is carried out the dry method enzymolysis, the 100ml enzyme can be separated 60～200U konjaku powder, and hydrolysis temperature is 45-60 ℃, and enzymolysis time is 6-12 hour;

(2) the smart powder behind the enzymolysis is carried out deactivation sterilization and wet method mechanical mill;

(3) the smart powder after grinding is handled with alcohol mixeding liquid, kept throw out;

(4) in the throw out that stays, add the alcohol mixed solvent that contains sequestrant, fully stir, remove mixed solvent behind the immersion certain hour, handle with ethanolic soln again, also keep throw out;

(5) add pure water in above throw out, stirring is left standstill, and makes its abundant swelling, carries out purifying treatment repeatedly in swelling process;

(6) with the swelling thing after concentrating under the rotatory evaporator, add weight percent concentration and be the ethanol 95% or 95% or more, stir, make konjac glucomanna that the oyster white flocks take place in ethanol, centrifugation precipitates again, obtains the sedimentary konjac glucomanna product that wet;

(7) the wet product of konjac glucomanna are carried out drying by the low-temperature vacuum drying method, obtain white powder finished product konjac glucomanna after the grinding.

From above-mentioned processing method as can be known, the present invention has adopted technology simple, be easy to industrial dry method enzymolysis, remove heavy metal ion by the sequestrant mixed solution, and utilize konjac glucomanna can swell in the characteristic of water, by the purification of water separation konjac glucomanna is swollen in and carry out the purification of water processing in the water process, because konjac glucomanna swelling object is long-pending very big, the present invention has adopted elder generation that the konjac glucomanna swelling thing behind the purification of water is concentrated, use extraction using alcohol again, make konjac glucomanna in alcoholic solution, form precipitation, further removal can be dissolved in alcoholic acid impurity, has guaranteed the purity of konjac glucomanna.Because of in the used technology of the present invention both without high temperature, also water gaging and ethanol greatly so this method can not damaged the molecular chain of konjac glucomanna, can not only obtain highly purified konjac glucomanna, and high viscosity, the medicinal characteristic of konjac glucomanna and the appearance luster of product have been kept.

Process for extracting to high-purity kanjak glucomannosan provided by the invention elaborates below.

At first, konjaku powder is carried out the dry method enzymolysis, promptly can separate the standard of 60～200U konjaku powder according to the 100ml enzyme, according to the smart powder amount of wanting enzymolysis,, add in the dry smart powder an amount of enzyme furnishing enzyme solution, fully stir evenly, make smart powder at 45～60 ℃ of following abundant enzymolysis 6～12 o'clock.Smart powder behind the enzymolysis is carried out the deactivation germicidal treatment, remove pathogenic bacterium, and smart powder carried out wet method mechanical mill, smart powder after will grinding then places ethanolic soln, discard impurity such as the soluble sugar that dissolves in the ethanol, fat, starch, keep the shallow lake thing, in this throw out, add the alcohol mixed solvent that contains sequestrant again, fully stir the back and soak certain hour, remove mixture, metal ions such as lead, arsenic and sulfurous gas, oxide compound etc. just are eliminated with mixture, and wherein sequestrant can be selected tartrate, acetic acid, phosphoric acid, citric acid or EDTA for use; And then carry-over handled with ethanolic soln, further remove protein, starch, Mierocrystalline celluloses etc. can be dissolved in the impurity of alcohol, after adding alcohol mixeding liquid, add pure water in the throw out of gained, stirring is left standstill, make its abundant swelling, and in the swelling process, it is purified repeatedly, with the swelling thing be placed in the rotatory evaporator concentrate after, be placed on weight percent concentration and be in the ethanolic soln more than 95% or 95%, make konjac glucomanna in ethanol, form the oyster white flocks, with centrifugal separation with precipitate and separate, just obtain the wet product of konjac glucomanna, at last with the wet product low-temperature vacuum drying of konjac glucomanna, what obtain after the grinding is white powder finished product konjac glucomanna.

Claims (2)

1, a kind of extraction process of high-purity kanjak glucomannosan, its technical process is:

(1) konjaku powder is carried out the dry method enzymolysis, the 100ml enzyme can be separated 60～200U konjaku powder, and hydrolysis temperature is 45-60 ℃, and enzymolysis time is 6-12 hour;

(2) the smart powder behind the enzymolysis is carried out deactivation sterilization and wet method mechanical mill;

(3) the smart powder after grinding is handled with alcohol mixeding liquid, kept throw out;

(4) in the throw out that stays, add the mixed solvent that contains sequestrant, fully stir, remove mixed solvent behind the immersion certain hour, handle with alcohol mixeding liquid again, also keep throw out;

(5) add pure water in above throw out, stirring is left standstill, and makes its abundant swelling, carries out purifying treatment repeatedly in swelling process;

(6) will be after concentrating under the rotatory evaporator to the swelling thing, the adding weight percent concentration is the ethanol more than 95% or 95%, stirs, and makes konjac glucomanna that the oyster white flocks take place in ethanol, the centrifugation precipitation obtains the wet product of sedimentary konjac glucomanna again;

(7) the wet product of konjac glucomanna are carried out drying by the low-temperature vacuum drying method, obtain white powder finished product konjac glucomanna after the grinding.

2, the extraction process of high-purity kanjak glucomannosan according to claim 1 is characterized in that: sequestrant can be selected tartrate, acetic acid, phosphoric acid, citric acid or EDTA for use.