CN117302810A - Biological sample cryopreservation device - Google Patents

Abstract

The invention relates to the technical field of biomedical storage, in particular to a biological sample freezing storage device, which aims to solve the problems that the tube picking structures in the existing intelligent biological sample library all adopt independent tube picking positions, the occupied space is large, the actions are more and the efficiency is low. The biological sample cryopreservation device comprises a box body, wherein a storage area and a cache area are arranged in the box body, the cache area is provided with a first conveying line and a code reading platform, the code reading platform is positioned on one side of the first conveying line, and a selection position is formed by the code reading platform and a retraction position of the first conveying line together and is used for selecting a cryopreservation box or a cryopreservation pipe. When the selecting position is used for selecting the frozen storage pipe, the code reading platform and the retrieving position of the first conveying line can be combined to form the pipe selecting position, so that the frozen storage pipes in the sample frozen storage box and the target frozen storage box can be mutually transferred, the pipe selecting station is prevented from being additionally arranged, the space occupation is reduced, and the working efficiency is improved.

Description

Biological sample cryopreservation device

Technical Field

The invention relates to the technical field of biomedical storage, and particularly provides a biological sample freezing and storing device.

Background

In the biomedical industry, an ultralow-temperature automatic refrigerator is required to store biological samples for freezing and storing, and the biological samples need to be recorded in information when entering and exiting the warehouse so as to manage the frozen biological samples. In the related art, an intelligent biological sample library is provided, a plurality of automatic refrigerators are arranged inside, the plurality of automatic refrigerators are divided into a machine type with a tube picking function and a machine type with a box picking function, and the plurality of automatic refrigerators are connected in series by using an automatic trolley carrying a transfer barrel, so that the transfer of a freezing box and/or a freezing tube can be realized among the plurality of automatic refrigerators. However, the tube picking structures in the intelligent biological sample library all adopt independent tube picking positions, so that the occupied space is large, the actions are more, and the efficiency is low.

Therefore, the present invention needs to provide a new biological sample cryopreserving device to solve the above technical problems.

Disclosure of Invention

The invention aims to solve the technical problems that the tube picking structures in the existing intelligent biological sample library all adopt independent tube picking positions, the occupied space is large, the actions are more and the efficiency is low.

For this purpose, the invention provides a biological sample cryopreservation device, which comprises a box body, wherein a storage area and a buffer area are arranged in the box body, the storage area is used for storing a cryopreservation box, the buffer area is provided with a first conveying line and a code reading platform, the first conveying line is arranged to be capable of conveying the cryopreservation box between the buffer area and the storage area or between the outside of the box body and the buffer area, the code reading platform is positioned on one side of the first conveying line and used for placing the cryopreservation box to read sample information of the cryopreservation box, and the code reading platform and a retracting position of the first conveying line jointly form a selecting position for selecting the cryopreservation box or the cryopreservation pipe.

Under the condition of adopting the technical scheme, when selecting the position to select the cryopreservation pipe operation, the retrieval position of the code reading platform and the first conveying line can be combined to form a pipe picking position, the code reading platform is used for placing a sample cryopreservation box or a target cryopreservation box taken out from a storage area, and the retrieval position of the first conveying line is used for placing the target cryopreservation box or the sample cryopreservation box taken out from the storage area, so that the sample cryopreservation box and the cryopreservation pipe in the target cryopreservation box can be mutually transferred, the pipe picking station is prevented from being additionally arranged, the space occupation is reduced, and the working efficiency is improved.

In a specific embodiment of the biological sample cryopreserving device, the buffer area is further provided with a second conveying line, the second conveying line is located on one side of the code reading platform, one of the second conveying line and the first conveying line is arranged to be capable of conveying the cryopreserved box between the buffer area and the storage area, and the other is arranged to be capable of conveying the cryopreserved box between the outside of the box body and the buffer area.

In a specific embodiment of the biological sample cryopreserving device, a part, close to the code reading platform, in the buffer area and a part, close to the first conveying line, of the buffer area are both provided with a box pressing mechanism, and the box pressing mechanism is used for applying force to a corresponding cryopreservation box to position when a cryopreservation pipe is selected, so that the cryopreservation box does not move in the pipe selecting process.

Under the condition of adopting the technical scheme, when selecting the frozen storage pipe, the frozen storage box mechanism is utilized to apply force to the frozen storage box on the corresponding code reading platform and the frozen storage box which is retracted on the first conveying line so as to position the frozen storage box, so that the frozen storage box does not follow movement when the frozen storage box is selected, the success of pipe selection is ensured, and the problem that the frozen storage box is caused to follow movement to cause pipe selection failure due to the adhesion of the frozen storage pipe and the frozen storage box when the frozen storage pipe is selected is avoided.

In a specific embodiment of the biological sample cryopreserving device, the box pressing mechanism comprises a driving assembly, a supporting frame and a first sliding plate, wherein the supporting frame is fixed in a buffer area of the box body, the driving assembly is arranged on the supporting frame, the first sliding plate is in sliding connection with the supporting frame, a clamp holder is fixed at one end of the first sliding plate, and the driving assembly drives the first sliding plate to do reciprocating linear motion so as to drive the clamp holder to clamp the cryopreserving box or separate from the cryopreserving box.

Under the condition of adopting the technical scheme, the first sliding plate is driven by the driving assembly to drive the clamp holder to move, and the clamp holder can clamp the freezing box in the moving process, so that the purpose that the freezing box does not move when the pipe is picked is achieved, the clamp holder clamps the side wall of the freezing box, the freezing box is applicable to the freezing boxes with different heights, and the application range is wider.

In a specific embodiment of the biological sample cryopreserving device, the code reading platform and the first conveying line are both provided with placing grooves for placing the cryopreserving box, one end of the first sliding plate is provided with a notch, the clamp comprises two elastic pieces, the two elastic pieces are respectively fixed on two opposite side walls in the notch, a clamping opening is formed between the two elastic pieces, and the two elastic pieces are extruded by the cryopreserving box in the process of moving along the first sliding plate towards the cryopreserving box to generate elastic force so that the clamping opening clamps the cryopreserving box.

Under the condition of adopting the technical scheme, the elastic clamping freezing box with the two elastic sheets is simple in integral structure and low in manufacturing cost.

In a specific embodiment of the biological sample cryopreservation apparatus, the notch and the clamping opening are both V-shaped.

In a specific embodiment of the biological sample cryopreserving device, the elastic sheet is V-shaped, one side wall of the elastic sheet is fixed on the side wall of the first sliding plate, and the other side wall of the elastic sheet is located in the notch.

In the specific embodiment of the biological sample cryopreserving device, the buffer area is further provided with a mechanical arm, the mechanical arm is located above the first conveying line, the second conveying line and the code reading platform, and the mechanical arm is arranged to be capable of clamping the cryopreserving box and/or transferring the cryopreserving tube among the first conveying line, the second conveying line and the code reading platform.

In a specific embodiment of the biological sample cryopreserving device, the mechanical arm comprises a tube picking clamp, a box picking clamp and a three-way displacement driving mechanism, wherein the three-way displacement driving mechanism is arranged to drive the tube picking clamp and the box picking clamp to respectively move along the XYZ axis direction, the tube picking clamp is used for clamping a cryopreserving tube, and the box picking clamp is used for clamping the cryopreserving box.

Under the condition of adopting the technical scheme, the picking pipe clamp device and the picking box clamp device can be driven to move along the XYZ axis directions respectively by utilizing the three-direction driving mechanism, so that the volume of the whole structure is reduced, and the occupied space is small.

In a specific embodiment of the biological sample cryopreserving device, the storage area and the buffer area are closed cavities which are independent of each other, a transmission window which can be communicated with the buffer area is arranged on the storage area, and a picking and placing window which is communicated with the buffer area in the storage area is arranged on the side wall of the box body.

Drawings

Preferred embodiments of the present invention are described below with reference to the accompanying drawings, in which:

FIG. 1 is a schematic diagram of the whole structure of a biological sample cryopreservation apparatus provided by the invention;

FIG. 2 is a schematic diagram of the structure of a buffer area in a box;

FIG. 3 is a schematic view of the structure of FIG. 2 at another angle;

FIG. 4 is a schematic view of the structure of the first conveyor line;

FIG. 5 is a schematic view of the structure of FIG. 4 at another angle;

FIG. 6 is a schematic view of the structure of the picking cassette holder;

FIG. 7 is a schematic view of the structure of the gripper;

FIG. 8 is a schematic structural view of the cartridge pressing mechanism;

FIG. 9 is an enlarged view of a portion of FIG. 8A;

fig. 10 is a schematic structural view of the first load table.

List of reference numerals:

1. a case; 2. a second conveyor line; 3. a first conveyor line; 31. a first bracket; 32. a second slide plate; 33. a first load table; 331. a supporting rod; 332. a placement groove; 34. a first horizontal movement mechanism; 341. a first motor; 342. a first rack; 343. a first slide rail; 35. a second horizontal movement mechanism; 351. a chain; 352. a sprocket; 4. a code reading platform; 41. a second load table; 42. a second bracket; 5. a box pressing mechanism; 51. a first slide plate; 52. a support frame; 53. a holder; 54. a drive assembly; 541. a third motor; 542. a third rack; 543. a third gear; 6. a three-dimensional movement driving mechanism; 61. a first orientation driving mechanism; 62. a second azimuth driving mechanism; 63. a third position driving mechanism; 7. picking a box clamp holder; 8. a tube gripper; 9. an electric door.

Detailed Description

Preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are merely for explaining the technical principles of the present invention, and are not intended to limit the scope of the present invention. Those skilled in the art can adapt it as desired to suit a particular application.

It should be noted that, in the description of the present invention, terms such as "upper," "lower," "inner," "outer," and the like, which indicate a direction or a positional relationship, are based on the direction or the positional relationship shown in the drawings, which are merely for convenience of description, and do not indicate or imply that the relevant devices or elements must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the ordinal terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

Furthermore, it should be noted that, in the description of the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium. The specific meaning of the above terms in the present invention can be understood by those skilled in the art according to the specific circumstances.

The tube picking structures in the existing intelligent biological sample library all adopt independent tube picking positions, so that the occupied space is large, the actions are more, and the efficiency is low.

In order to solve the above technical problems, referring to fig. 1 and 2, the present invention provides a biological sample cryopreservation apparatus, which includes a box 1, a storage area and a buffer area are provided in the box 1, the storage area is used for storing a cryopreservation box, the buffer area is provided with a first conveying line 3 and a code reading platform 4, the first conveying line 3 is configured to be capable of transferring the cryopreservation box between the buffer area and the storage area or between the outside of the box 1 and the buffer area, the code reading platform 4 is located at one side of the first conveying line 3, and is used for placing the cryopreservation box to read sample information of the cryopreservation box, and the code reading platform 4 and a retraction position of the first conveying line 3 together form a selection position for selecting the cryopreservation box or a cryopreservation tube.

Specifically, the storage area and the buffer area are closed cavities which are mutually independent, the storage area and the buffer area are provided with mutually independent refrigerating systems, the storage area is kept at-80 ℃ by adopting hydrocarbon refrigeration, the buffer area is kept at-25 ℃ by adopting hydrocarbon air cooling, and the refrigerating temperature can be flexibly adjusted according to requirements. The storage area is provided with a transfer window which can be communicated with the cache area, and the side wall of the box body 1 is provided with a picking and placing window which is communicated with the cache area therein. The power door 9 is arranged on the transmission window and the picking and placing window. The construction of the power door 9 is known to those skilled in the art and will not be described in detail herein.

The storage area is provided with a double-row goods shelf and a mechanical arm, the double-row goods shelf is provided with a plurality of storage positions, and each storage position is matched with the freezing storage box. Therefore, the freezing box is stored in the storage position of the double-row shelf, and the freezing box is convenient to take and place. The arrangement of the double-row shelf can improve the storage quantity, so that the capacity of the biological sample freezing and storing device is further increased. Illustratively, a biological sample to be frozen is stored in a freezing tube, a plurality of freezing tubes are integrally stored in a freezing box, and then the freezing box is placed in a storage place for storage. The manipulator is arranged at the middle part of the double-row goods shelves and is used for taking and placing the freezing storage boxes of the storage positions at two sides. Therefore, when the frozen sample is taken and placed, the movement stroke of the manipulator is reduced. The specific structure of the manipulator is known to those skilled in the art and will not be described in detail herein.

In one embodiment, referring to fig. 2, the buffer area is further provided with a second conveying line 2, the second conveying line 2 is located at one side of the code reading platform 4, and when the first conveying line 3 is configured to be capable of conveying the frozen box between the buffer area and the storage area, the second conveying line 2 is configured to be capable of conveying the frozen box between the outside of the box 1 and the buffer area.

In another embodiment, the buffer area is further provided with a second conveying line 2, the second conveying line 2 is located at one side of the code reading platform 4, and when the first conveying line 3 is set to be capable of conveying the freezing box between the outside of the box body 1 and the buffer area, the second conveying line 2 is set to be capable of conveying the freezing box between the buffer area and the storage area.

In the present application, the first conveyor line 3 is arranged to be able to transfer the cryopreserved cassettes between the buffer area and the storage area, and the second conveyor line 2 is arranged to be able to transfer the cryopreserved cassettes between the outside of the casing 1 and the buffer area, as a preferred embodiment.

Specifically, referring to fig. 4 and 5, the first conveying line 3 includes a first bracket 31, a second sliding plate 32, a first horizontal moving mechanism 34, a second horizontal moving mechanism 35 and a first loading platform 33, the first bracket 31 is fixed on a side wall of a buffer area in the box 1, the second sliding plate 32 is slidably connected with the first bracket 31, the first horizontal moving mechanism 34 drives the second sliding plate 32 to perform horizontal linear movement, the second horizontal moving mechanism 35 is arranged on the second sliding plate 32, the first loading platform 33 is slidably connected on the second sliding plate 32, the second horizontal moving mechanism 35 can drive the first loading platform 33 to move relative to the second sliding plate 32 in the moving process of the second sliding plate 32, so that the horizontal moving distance of the first loading platform 33 is prolonged, the first loading platform 33 can extend into the outer side of the box 1 through a first picking and placing window, the whole size of the retracted first conveying line 3 is small, the space occupation is small while the transportation of the frozen box is realized, and the space occupation rate is reduced. The first conveyor line 3 and the second conveyor line 2 have the same structure, and a detailed description thereof will not be repeated here.

The first horizontal moving mechanism 34 includes a first motor 341, a first slide rail 343, a first rack 342, and a first gear, the first motor 341 is fixed on the first bracket 31, the first slide rail 343 is slidably connected on the first bracket 31, the second slide plate 32 is fixed on the first slide rail 343, the first rack 342 is fixed on a side wall of the second slide plate 32, and an output shaft of the first motor 341 is fixed with the first gear engaged with the first rack 342. The first gear is rotated by the first motor 341, so that the second slider 32 moves in a horizontal straight line.

The second horizontal moving mechanism 35 comprises a chain 351 and two chain wheels 352, the two chain wheels are respectively connected to two ends of the second sliding plate 32 in a rotating mode, the chain 351 is sleeved on the chain wheels 352, the second sliding plate 32 penetrates through the inner side of the chain 351, part of the chain 351 is located above the second sliding plate 32, part of the chain 351 is located below the second sliding plate 32, a connecting block is fixed on the lower chain 351, and the connecting block is fixedly connected with the first bracket 31. The second sliding plate 32 drives the sprocket to move forward in the horizontal linear movement process, the sprocket rotates under the condition that the sprocket is matched with the chain 351, and the upper part of the chain 351 drives the first loading table 33 to move linearly relative to the second sliding plate 32, so that the purpose of lengthening the movement distance of the first loading table 33 is achieved, and meanwhile, the occupied space is reduced and the manufacturing cost is reduced.

In addition, the code reading platform includes a second support 42, a second loading table 41, and a code reader (not shown), where the second loading table 41 is fixed on the second support 42, the second support 42 is fixed in the box 1, and the code reader is located above the second loading table 41 and fixedly connected with the second support 42. It should be noted that the specific structure of the code reader is known to those skilled in the art, and the present application only uses the function thereof, so that detailed description thereof is omitted herein.

The first loading table 33 and the second loading table 41 are each provided with a placement groove 332 for placing the freezing box. Specifically, referring to fig. 10, the four corner ends on the first loading platform 33 are respectively fixed with a supporting rod 331, the top ends of the supporting rods 331 are fixed with stoppers, and the inner sides of the four stoppers form a placing groove 332, so that the freezing box is placed in the placing groove 332, and the freezing box is prevented from falling down from the supporting rods 331, but in order to make the freezing box easy to place, a gap exists between the stoppers and the freezing box.

The second loading table 41 has the same structure as the first loading table 33, and the retracted position on the first conveyor line 3 is a position where the first loading table 33 is retracted to the buffer and corresponds to the second loading table 41.

When the selecting position is used for selecting the frozen storage pipe, the code reading platform and the retrieving position of the first conveying line 3 can be combined to form the pipe selecting position, the code reading platform is used for placing the sample frozen storage box or the target frozen storage box taken out of the storage area, and the retrieving position of the first conveying line 3 is used for placing the target frozen storage box or the sample frozen storage box taken out of the storage area, so that the sample frozen storage box and the frozen storage pipe in the target frozen storage box can be mutually transferred, the pipe selecting station is prevented from being additionally arranged, the space occupation is reduced, and the working efficiency is improved.

In one embodiment, the buffer area is further provided with a mechanical arm, which is located above the first conveying line 3, the second conveying line 2 and the code reading platform 4, and the mechanical arm is configured to be capable of clamping the cryopreserved box and/or transferring the cryopreserved tube between the first conveying line 3, the second conveying line 2 and the code reading platform 4.

Alternatively, referring to fig. 3, 6 and 7, the mechanical arm includes a picking gripper 8, a picking cassette gripper 7 and a three-way displacement driving mechanism 6, the three-way displacement driving mechanism 6 is configured to be capable of driving the picking gripper 8 and the picking cassette gripper 7 to move along XYZ axis directions, the picking gripper 8 is used for gripping a frozen storage tube, and the picking cassette gripper 7 is used for gripping a frozen storage cassette.

Specifically, the three-way displacement driving mechanism 6 includes a first azimuth driving mechanism 61, a second azimuth driving mechanism 62, and two third azimuth driving mechanisms 63, the first azimuth driving mechanism 61 driving the second azimuth driving mechanism 62 and the two third azimuth driving mechanisms 63 to move together in the X-axis direction (in fig. 2, to move in the lateral direction), the second azimuth driving mechanism 62 driving the third azimuth driving mechanism 63 to move in the Y-axis direction (in fig. 2, to move in the longitudinal direction), the two third azimuth driving mechanisms 63 are respectively connected to the picking cassette holder 7 and the picking cassette holder 8, and the third azimuth driving mechanism 63 drives the corresponding picking cassette holder 7 or the picking cassette holder 8 to move in the Z-axis direction (in fig. 2, to move up and down). The number of the second azimuth driving mechanisms 62 may be one or two, and when the number of the second azimuth driving mechanisms is one, the second azimuth driving mechanisms 62 jointly drive the two third azimuth driving mechanisms 63 to move; when there are two, the two second azimuth driving mechanisms 62 drive one third azimuth mechanism to move, respectively. The first azimuth driving mechanism 61, the second azimuth driving mechanism 62, and the third azimuth driving mechanism 63 may be electric modules or rack-and-pinion driving mechanisms, and the present application is not particularly limited with respect to specific structures, and is flexibly set without departing from the basic principles of the present application. In addition, the structure of the electric module using the screw principle is known to those skilled in the art, and will not be described in detail herein.

Illustratively, when the first azimuth driving mechanism 61 is a rack-and-pinion driving mechanism, the first azimuth driving mechanism specifically includes a third bracket, a second gear, a second rack, a second motor and a second sliding rail, the third bracket is fixed on the inner wall of the box 1, the second sliding rail and the second rack are both fixed on the third bracket, a first sliding block is slidingly connected on the second sliding rail, the second motor is fixed on the first sliding block, an output shaft of the second motor is fixed with the second gear, the second gear is meshed with the second rack, and the second motor drives the second gear to rotate to realize the sliding of the first sliding block along the second sliding rail. A fourth bracket is fixed on the first sliding block and is perpendicular to the third bracket, and two side walls of the fourth bracket are respectively connected with a second azimuth driving mechanism 62. The second azimuth driving mechanism 62 has the same structure as the first azimuth driving mechanism 61, the third azimuth driving mechanism 63 adopts an electric module, at this time, a first sliding block on the second azimuth driving mechanism 62 fixes an electric module, and a sliding block on the electric module fixes the picking box holder 7 or the picking pipe holder 8 corresponding to the first sliding block. The picking cassette holder 7 and the picking tube holder 8 are located on both sides of the fourth bracket.

It should be noted that the specific structures of the picking box gripper 7 and the picking tube gripper 8 are known to those skilled in the art, and are not described in detail herein.

According to the invention, the picking tube clamp holder 8 and the picking box clamp holder 7 can be driven to move along the XYZ axis directions respectively by using a three-way driving mechanism, so that the volume of the whole structure is reduced, and the occupied space is small.

In one embodiment, referring to fig. 2, a part of the buffer area, which is close to the code reading platform, and a part of the buffer area, which is close to the first conveying line 3, are both provided with a box pressing mechanism 5, and the box pressing mechanism 5 is used for applying force to a corresponding freezing box to position when the freezing box is selected, so that the freezing box does not move in the process of selecting the freezing box.

When the frozen storage pipes are selected, the frozen storage boxes on the corresponding code reading platform and the frozen storage boxes in the first conveying line 3 are subjected to force application by the box pressing mechanism 5 so as to be positioned, so that the frozen storage boxes do not move along with the pipe selecting process, the success of pipe selecting is ensured, and the problem that the frozen storage boxes fail to select the pipes due to the fact that the frozen storage pipes are stuck with the frozen storage boxes due to frost when the frozen storage pipes are selected is avoided.

In one embodiment, referring to fig. 8 and 9, the box pressing mechanism 5 includes a driving component 54, a supporting frame 52 and a first sliding plate 51, the supporting frame 52 is fixed in the buffer area of the box body 1, the driving component 54 is arranged on the supporting frame 52, the first sliding plate 51 is slidably connected with the supporting frame 52, a clamp 53 is fixed at one end of the first sliding plate 51, and the driving component 54 drives the first sliding plate 51 to make a reciprocating linear motion to drive the clamp 53 to clamp the freezing box or separate from the freezing box.

Specifically, the driving assembly 54 includes a third motor 541, a third rack 542, and a third gear 543, where the third motor 541 is fixed on the support frame 52, the third rack 542 is fixed on the first sliding plate 51, the third gear 543 is fixed on an output shaft of the third motor 541, and the third gear 543 is meshed with the third rack 542, and the third motor 541 drives the third gear 543 to rotate, so that the third rack 542 drives the first sliding plate 51 to perform a reciprocating linear motion. It will be appreciated, of course, that the particular configuration of the drive assembly 54 is not limited to that described above, but is flexible in terms of practical requirements without departing from the basic principles of the present invention.

The invention uses the driving component 54 to drive the first sliding plate 51 to drive the clamp holder 53 to move, and the clamp holder 53 can clamp the freezing box in the moving process, so that the purpose that the freezing box does not move when the pipe is picked is achieved, and the clamp holder 53 clamps the side wall of the freezing box, so that the invention is applicable to the freezing boxes with different heights, and has wider application range.

In one embodiment, the code reading platform and the first conveying line 3 are both provided with a placing groove 332 for placing the freezing box, one end of the first sliding plate 51 is provided with a notch, the clamp 53 comprises two elastic pieces, the two elastic pieces are respectively fixed on two opposite side walls in the notch, a clamping opening is formed between the two elastic pieces, and the two elastic pieces are extruded by the freezing box in the process of moving along the first sliding plate 51 towards the freezing box to generate elastic force so that the clamping opening clamps the freezing box. The placement groove 332 can prevent that the cryopreservation box from dropping down, has the clearance between placement groove 332 and the cryopreservation box, in order to put into the cryopreservation box more easily. In order to ensure the success of tube picking, the freezing box does not move along with the freezing tube during tube picking, so that the freezing box needs to be fixed during tube picking. The two elastic sheets form a clamping opening, the first sliding plate 51 drives the clamping opening to approach the freezing box to enable the part of the freezing box to enter the clamping opening, the freezing box extrudes the elastic sheets under the blocking effect of the placing groove 332, the two elastic sheets generate elastic force and act on the freezing box, the purpose of clamping and fixing the freezing box is achieved, and meanwhile the manufacturing cost of the clamp holder 53 is low.

It will be appreciated, of course, that the construction of the holder 53 is not limited to that described above, and that other constructions may be chosen without departing from the basic principles of the invention, and that the holder 53 may illustratively be in the form of a suction cup for holding it, but the construction described above is considered as a preferred embodiment of the present application, and is of simple construction and low cost to manufacture.

Specifically, as shown in fig. 9, the notch is V-shaped with the clamping opening, the elastic sheet is also V-shaped, the freezing box is rectangular, one side of the elastic sheet is fixed on the first sliding plate 51, the other side is located in the notch and is in a free state, the first sliding plate 51 is inserted into the clamping opening toward the freezing box during the moving process of the freezing box, and the side of the elastic sheet in the free state is extruded to generate clamping force on the freezing box, so that the purpose of clamping the freezing box is achieved. In addition, the clamps 53 clamp two adjacent side walls of the freezing box, so that the height of the freezing box is not limited, and the freezing box is applicable to freezing boxes with different heights and has wider application range.

It will be appreciated that the shapes of the clamping opening, the elastic sheet and the notch are not particularly limited in the application, and are flexibly set according to actual use requirements without departing from the basic principle of the invention.

In the present application, the operation steps of the present application will be described by taking the case of storing a sample in a storage area after the sample is stored in the storage area after passing through the storage window and entering the buffer area of the case 1. The first conveying line 3 is used for the transmission of cryopreserved box between buffer memory district and the storage area, specifically, gets to put the electrically operated gate 9 of window and opens, and the first loading platform 33 on the second conveying line 2 stretches out to get the outside of putting the window, places the sample cryopreserved box on first loading platform 33 through manual work or robot, and rethread second conveying line 2 is with the sample cryopreserved box transport to the buffer memory district in the box 1, gets to put the electrically operated gate 9 of window and closes this moment. Meanwhile, the electric door 9 on the transfer window of the storage area is opened, the first loading platform 33 on the first conveying line 3 stretches into the storage area, the target frozen storage box is taken out of the storage position through the manipulator and placed on the first loading platform 33, then the first conveying line 3 transfers the target box to the buffer area and is located in the retraction position of the first conveying line 3, and the electric door 9 of the transfer window is closed. The mechanical arm is started to drive the box picking clamp holder 7 to move, after the box picking clamp holder 7 places a target frozen storage box on the code reading platform for code reading, the target frozen storage box is placed on the first loading table 33 of the first conveying line 3 again, then the mechanical arm drives the box picking clamp holder 7 to clamp the sample frozen storage box to be placed on the code reading platform for code reading, meanwhile, the box pressing mechanism 5 is started to position the frozen storage box, and then the mechanical arm drives the box picking clamp holder 8 to pick a frozen storage pipe on the sample frozen storage box into the target frozen storage box. The electric door 9 of the transfer window is opened after the tube picking is completed, the first conveying line 3 sends the target frozen storage box back to the storage area, the electric door 9 is closed after the first conveying line 3 is retracted, the mechanical arm drives the tube picking clamp holder 7 to move to place the empty sample frozen storage box on the first load table 33 of the second conveying line 2, the electric door 9 of the picking and placing window is opened, the second conveying line 2 conveys the empty sample frozen storage box, the electric door 9 is closed after the second conveying line 2 is retracted, and tube picking operation is completed.

Thus far, the technical solution of the present invention has been described in connection with the preferred embodiments shown in the drawings, but it is easily understood by those skilled in the art that the scope of protection of the present invention is not limited to these specific embodiments. Equivalent modifications and substitutions for related technical features may be made by those skilled in the art without departing from the principles of the present invention, and such modifications and substitutions will fall within the scope of the present invention.

Claims (10)

1. The utility model provides a biological sample freezes and deposits device, its characterized in that, biological sample freezes and deposits device includes the box, be provided with storage area and buffer zone in the box, the storage area is used for depositing the cryopreserved box, the buffer zone is provided with first transfer chain and code reading platform, first transfer chain sets up to can with the cryopreserved box the buffer zone with transfer or set up to can with the cryopreserved box outside the box with transfer between the buffer zone, code reading platform is located one side of first transfer chain for place the cryopreserved box in order to read the sample information of cryopreserved box, code reading platform with the position of retrieving of first transfer chain forms jointly and selects the position and be used for choosing the cryopreserved box or freezes the pipe operation.

2. The biological sample cryopreservation apparatus of claim 1, wherein the buffer zone is further provided with a second conveyor line, the second conveyor line is located at one side of the code reading platform, one of the second conveyor line and the first conveyor line is configured to be capable of transferring a cryopreservation box between the buffer zone and the storage zone, and the other is configured to be capable of transferring a cryopreservation box between the outside of the case and the buffer zone.

3. The biological sample cryopreservation device of claim 1, wherein a part of the buffer area, which is close to the code reading platform, and a part of the buffer area, which is close to the first conveying line, are both provided with a box pressing mechanism, and the box pressing mechanism is used for applying force to a corresponding cryopreservation box to position when a cryopreservation tube is selected, so that the cryopreservation box does not move in the tube selecting process.

4. The frozen storage device of claim 3, wherein the box pressing mechanism comprises a driving assembly, a supporting frame and a first sliding plate, the supporting frame is fixed in a buffer area of the box body, the driving assembly is arranged on the supporting frame, the first sliding plate is in sliding connection with the supporting frame, a clamp holder is fixed at one end of the first sliding plate, and the driving assembly drives the first sliding plate to do reciprocating linear motion so as to drive the clamp holder to clamp the frozen storage box or separate from the frozen storage box.

5. The frozen storage device of claim 4, wherein the code reading platform and the first conveying line are respectively provided with a placing groove for placing the frozen storage box, one end of the first sliding plate is provided with a notch, the clamp comprises two elastic pieces, the two elastic pieces are respectively fixed on two opposite side walls in the notch, a clamping opening is formed between the two elastic pieces, and the two elastic pieces are extruded by the frozen storage box in the process of moving along the first sliding plate towards the frozen storage box to generate elastic force so that the clamping opening clamps the frozen storage box.

6. The biological sample cryopreservation apparatus of claim 5 wherein the notch and the grip opening are both V-shaped.

7. The frozen storage device of claim 5, wherein the spring plate is V-shaped, one side wall of the spring plate is fixed on the side wall of the first slide plate, and the other side wall of the spring plate is positioned in the notch.

8. The frozen storage device of claim 1, wherein the buffer area is further provided with a mechanical arm, the mechanical arm is located above the first conveying line, the second conveying line and the code reading platform, and the mechanical arm is arranged to clamp the frozen storage box and/or transport the frozen storage pipe between the first conveying line, the second conveying line and the code reading platform.

9. The frozen storage device of claim 8, wherein the mechanical arm comprises a tube picking gripper, a box picking gripper and a three-way displacement driving mechanism, wherein the three-way displacement driving mechanism is configured to drive the tube picking gripper and the box picking gripper to move along the XYZ axis direction respectively, the tube picking gripper is used for clamping frozen storage tubes, and the box picking gripper is used for clamping frozen storage boxes.

10. The frozen storage device for biological samples according to any one of claims 1 to 9, wherein the storage area and the buffer area are closed cavities independent of each other, a transmission window which can be communicated with the buffer area is arranged on the storage area, and a picking and placing window which is communicated with the buffer area in the storage area is arranged on the side wall of the box body.