CN117286022B - Contrast experimental device for microorganisms in soil - Google Patents

Abstract

The invention relates to the field of microorganism experiments, in particular to a contrast experiment device for microorganisms in soil, which comprises a bottom plate, a placing cylinder, a telescopic cylinder, an upper cover, a fixing column and an environment conversion mechanism, wherein the bottom plate is arranged on the bottom plate; the invention can solve the following problems in the process of carrying out contrast experiments on soil in the prior art: the environment where the soil is located cannot be adjusted, so that the types and the quantity of microorganisms in the soil can be increased or reduced under the influence of the environment, the experimental precision and experimental diversity are influenced, and the research of soil samples is not facilitated; the invention can convert the environment state of the soil sample in the culture dish, and is particularly divided into a wet environment, a dry environment, a high-temperature environment and a low-temperature environment, so that the soil sample in different environments is subjected to a comparison experiment, the accuracy of the comparison experiment on the soil sample can be improved through converting the environment, the experiment effect is further improved, and the experiment diversity is improved.

Description

Contrast experimental device for microorganisms in soil

Technical Field

The invention relates to the field of microorganism experiments, in particular to a contrast experiment device for microorganisms in soil.

Background

Soil is one of the most important resources in natural environments, and it carries the growth and development of various organisms and plants. Wherein the soil microorganism is an indispensable part of the soil ecosystem; in order to detect the diversity of microorganisms and the stability of an ecosystem in soil, a comparison test needs to be performed on soil samples and standard samples in different regions and different depths, and the comparison test of microorganisms in soil generally comprises the aspects of the types and the numbers of the microorganisms in soil, activity and metabolism, physical and chemical properties of the soil, microbial community structure, nutrient conversion efficiency, water retention capacity and the like.

The type and the quantity of the microorganisms in the soil obtained through the comparison test reflect the complexity of a soil ecological system and the condition of natural environment, and the type and the quantity of the microorganisms in the soil can be different because the environment of the soil is different in different seasons and different weather; to ensure the accuracy of the comparative test, it is necessary to place the soil in a petri dish and simulate whether the types and the numbers of microorganisms in the soil are increased or decreased under different environments, thereby more comprehensively detecting the types and the numbers of microorganisms in the soil.

For comparison of the types and numbers of microorganisms in soil, a counting method is generally adopted, in which information about the number of microorganisms is obtained by observing the number and morphology of cells in a soil sample, and then the total number of microorganisms in soil is estimated by calculating the number of microorganisms.

However, when the existing soil is subjected to the comparison experiment, the soil sample is usually taken back to the laboratory and then is directly subjected to the comparison experiment, so that the existing soil comparison experiment method has some defects:

1. although the soil sample can be preserved by a refrigerator in a laboratory, the soil sample is also caused to be in different environments, so that the phenomenon that the variety and the quantity of microorganisms in the soil are increased or reduced is easy to occur; the existing comparison experiment method cannot adjust the environment of the soil, so that the types and the quantity of microorganisms in the soil can be increased or reduced under the influence of the environment, and the results obtained by the comparison experiment cannot represent the original types and the quantity of the microorganisms, so that the experiment precision is affected.

2. In addition, the types and the numbers of microorganisms in the soil samples under different environments are changed, so that corresponding environments are required to be manufactured for the soil samples according to experimental requirements, but the existing comparison experimental method cannot manufacture different environments for the soil samples, so that experimental diversity is affected, and the research of the soil samples is not facilitated.

Thus, under the above stated viewpoints, there is room for improvement in the comparative test means of existing microorganisms.

Disclosure of Invention

In order to solve the problems, the invention provides a contrast experiment device for microorganisms in soil, which comprises a bottom plate, wherein a plurality of placing cylinders are arranged above the bottom plate at equal intervals along the length direction, two telescopic cylinders are symmetrically arranged between the lower end of each placing cylinder and the upper end of the bottom plate, the upper end of each placing cylinder is clamped with an upper cover, fixing columns are arranged between the lower ends of four corners of the lower end of each upper cover and the upper end of the bottom plate, and the lower end of each upper cover is provided with an environment conversion mechanism positioned inside each placing cylinder.

The environment conversion mechanism comprises a water tank, the middle part of the lower end of an upper cover is provided with the water tank, the side wall of the water tank is provided with a water inlet pipe communicated with the inside of the water tank, the upper end of the water inlet pipe extends upwards after penetrating through the upper cover, the lower end of the water tank is uniformly provided with a plurality of annularly distributed lower inserting cylinders and drain pipes, the drain pipes are inserted into the lower inserting cylinders, the upper end of the drain pipes are communicated with the water tank, the upper end of the upper cover is provided with a conversion assembly, the conversion assembly comprises an installation block arranged in the middle of the upper end of the upper cover, a linkage assembly is installed between the installation block and the upper cover, the linkage assembly comprises positioning plates, and the upper end of the upper cover is symmetrically provided with two positioning plates along the installation block.

Preferably, the conversion assembly further comprises an execution column, the execution column parallel to the length direction of the execution column is penetrated in the installation block, a circular groove is formed in the installation block, an auxiliary branched chain positioned in the circular groove is installed between the execution column and the installation block, three installation grooves are formed in the installation block at equal intervals along the clockwise direction of the execution column, wires are arranged in the installation grooves through triggering branched chains, one end of each wire, far away from the triggering branched chain, sequentially penetrates through the upper cover and the water tank and then extends to the inside of the lower insertion barrel, the execution branched chain connected with the wires is installed in the lower insertion barrel, and waterproof sleeves sleeved outside the wires are installed in the water tank;

one side of the installation block, which is not provided with the installation groove, is penetrated and provided with a through hole communicated with the circular groove, and the inside of the through hole is provided with a ventilation hose communicated with the water tank and the outside.

Preferably, the auxiliary branched chain comprises an annular cylinder, the middle part in the circular groove is rotationally connected with the annular cylinder, the inner side wall of the annular cylinder is uniformly provided with a plurality of key teeth which are distributed in an annular way, one side of the outer wall of the execution column is sleeved with a driving sleeve, and the outer wall of the driving sleeve is uniformly provided with a plurality of key grooves corresponding to the key teeth in position;

an annular groove is formed in one side, away from the driving sleeve, of the annular cylinder, a flexible elastic sheet is arranged on any side of the outer wall of the annular cylinder, an annular plate sleeved on the outer wall of the execution column is rotatably connected to one side, away from the driving sleeve, of the inner portion of the annular groove, an annular pad in sliding fit with the annular groove is arranged on one side, close to the annular cylinder, of the annular plate, and a chamfer is formed in the outer wall, close to the annular groove, of the annular pad.

Preferably, the trigger branched chain comprises a fixed cylinder, a fixed cylinder is arranged in the mounting groove, the inner wall of the fixed cylinder is connected with a collision pin through a displacement plate in a sliding manner, a lead is connected with the upper end of the displacement plate, a supporting spring is arranged between the upper end of the displacement plate and the inner top wall of the fixed cylinder, one end of the collision pin, which is far away from the displacement plate, passes through the fixed cylinder and the mounting groove and then slides and collides with the annular cylinder, the lower end of the displacement plate is provided with a first electrode ring sleeved outside the collision pin, and the inner bottom wall of the fixed cylinder is provided with a second electrode ring matched with the first electrode ring.

Preferably, the execution branched chain comprises a controller, the lower ends of a plurality of wires are connected with the controller together, the lower end of the controller is uniformly provided with a plurality of connecting wires which are uniformly distributed and extend to the inside of the lower insertion barrel, the outer wall of the drain pipe is uniformly spaced from top to bottom and is circumferentially provided with a plurality of spray heads connected with the connecting wires, the outer wall of the lower insertion barrel is uniformly provided with a plurality of through holes corresponding to the spray heads in position, and the inner wall of each through hole is provided with a soil-proof cover;

a plurality of annular distributed holding tanks are uniformly arranged on the inner wall of the lower inserting cylinder, heating pads connected with connecting wires are arranged inside the holding tanks, a plurality of condensing pipes corresponding to the positions of the heating pads are uniformly arranged on the outer wall of the lower inserting cylinder, and the condensing pipes are connected with the connecting wires.

Preferably, the linkage assembly further comprises a fixed sleeve, two ends of the execution column penetrate through the mounting blocks and are rotationally connected with the positioning plate, the ends of two adjacent execution columns are respectively provided with the fixed sleeve and a rotating column, the rotating columns are rotationally abutted to the inside of the fixed sleeve, a yielding groove is formed in the rotating column, a push rod is slidably penetrated in the execution column, one end of the push rod is provided with a round table block which is slidably abutted to the yielding groove, a contraction spring sleeved outside the push rod is arranged between the round table block and the yielding groove, and the push rod in the same yielding groove is connected with the opposite round table block;

the inside displacement groove that evenly has offered a plurality of annular distributions and be linked together with the groove of stepping down of rotating the cylinder, and sliding connection has the grafting round pin in the displacement groove, and grafting round pin outer wall cover is equipped with the linkage board, is provided with the extrusion spring between one side that the linkage board kept away from the groove of stepping down and the displacement inslot wall, and the one end that the grafting round pin is close to the groove of stepping down slides the butt with the round platform piece, and a plurality of spacing grooves corresponding with the displacement groove position have evenly been offered to fixed sleeve inside wall, carry out and install spacing branched chain between post and the locating plate.

Preferably, the spacing branched chain still includes annular seat, and execution post outer wall is equipped with two annular seats along locating plate symmetry slip cap, rotates between annular seat and the locating plate to be connected, and annular seat outer wall has a plurality of annular swinging arms that distribute through the torsional spring evenly articulated, and one side that the swinging arms is close to the locating plate is provided with spacing ball, and a plurality of recess that contradict with spacing ball slip are seted up to the locating plate outer wall.

Preferably, the spacing branched chain includes spacing card, is located and evenly installs a plurality of annular spacing cards that distribute after the execution post tip of outside upper cover top passes the locating plate, and spacing card is L type structure, and the horizontal segment of spacing card is provided with the hem, and the inside push rod tip rotation of this execution post is provided with the carousel, and the carousel outer wall evenly is provided with a plurality of assistance bars corresponding with spacing card position.

Preferably, the positioning seat is arranged at the bottom of the placing cylinder, the clamping groove is formed in the upper end of the positioning seat, the culture dish is clamped in the clamping groove, and the upper half part of the clamping groove is gradually inclined to one side far away from the center of the positioning seat.

In summary, the present application includes at least one of the following beneficial technical effects:

1. according to the invention, the execution column drives the annular pad to be pulled out of the annular groove so that the flexible elastic sheet is sunken under the self elastic action, and then the execution column drives the annular cylinder to rotate, so that different environments can be manufactured on soil samples in the culture dish through the sunken flexible elastic sheet matched with the ventilation hose, the first electrode ring and the second electrode ring, and further, the soil samples in different environments can be subjected to a comparison experiment, so that the experiment effect is enhanced, and the experiment diversity is improved.

2. According to the invention, the first electrode ring and the second electrode ring are controlled to be contacted by rotating the annular cylinder, so that the environment state of the soil sample in the culture dish is converted, and the environment state is specifically divided into a wet environment, a dry environment, a high-temperature environment and a low-temperature environment, so that a comparison experiment is carried out on the soil sample in different environments, whether the types and the numbers of microorganisms in the soil sample are increased or decreased in different environments is judged according to experimental results, and the accuracy of the comparison experiment on the soil sample can be improved by converting the environments.

3. According to the invention, the locking state between the fixed sleeve and the rotary cylinder can be adjusted by adjusting the matching state between the plug pin and the limit groove, so that single control and multiple control can be formed, the single control cannot drive other execution columns to synchronously rotate when the execution column on any installation block rotates, and at the moment, the soil samples in different placing cylinders can be respectively subjected to environment conversion; the multi-control can drive other execution columns to synchronously rotate when rotating any execution column so as to simultaneously carry out environment conversion on soil samples in a plurality of placement cylinders, thereby carrying out self-adaptive adjustment on the control of the environment conversion according to actual experiment demands and being convenient to operate.

4. According to the invention, the push rod can be limited by matching the auxiliary bar and the limiting clamp, so that the plug pin is prevented from being pulled out of the limiting groove due to rebound of the push rod and the round table block under the action of the contraction spring, and the fixed sleeve and the rotary cylinder are ensured to be always kept in a locking state; and can carry out spacingly to the execution through the cooperation between spacing ball and the recess, prevent that the execution post from rotating at will and lead to flexible shell fragment to remove from contradicting the round pin department, and then cause to close the conversion to placing the inside soil sample environment of section of thick bamboo.

Drawings

The invention will be further described with reference to the drawings and examples.

Fig. 1 is a schematic structural view of the present invention.

Fig. 2 is a schematic view of the structure of the invention between the placement barrel, the upper cover and the environmental conversion mechanism.

Fig. 3 is a schematic view of the internal structure of the inventive placement barrel.

Fig. 4 is a schematic view of the structure between the upper cover and the environment switching mechanism of the present invention.

Fig. 5 is a schematic plan view of a conversion assembly of the present invention.

Fig. 6 is a schematic diagram of the internal structure of the environment switching mechanism of the present invention.

Fig. 7 is an enlarged view of a portion of fig. 6 a of the present invention.

Fig. 8 is an enlarged view of a portion of the invention at B of fig. 7.

Fig. 9 is a schematic view of the structure between the water tank and the actuating branched chain of the present invention.

Fig. 10 is an enlarged view of a portion of fig. 9C in accordance with the present invention.

Fig. 11 is a schematic view of the structure between the conversion assembly and the linkage assembly of the present invention.

Fig. 12 is a partial enlarged view of the invention at D of fig. 11.

Fig. 13 is an enlarged view of a portion of fig. 11 at E in accordance with the present invention.

In the figure, 1, a bottom plate; 2. placing a cylinder; 21. a positioning seat; 22. a culture dish; 3. a telescopic cylinder; 4. an upper cover; 5. fixing the column; 6. an environment conversion mechanism; 61. a water tank; 62. a water inlet pipe; 63. a lower insertion barrel; 64. a drain pipe; 65. a conversion assembly; 651. a mounting block; 652. performing a column; 653. auxiliary branched chains; 660. an annular cylinder; 661. key teeth; 662. a driving sleeve; 663. a key slot; 664. an annular groove; 665. a flexible spring plate; 666. an annular plate; 667. an annular pad; 654. triggering a branched chain; 670. a fixed cylinder; 671. a contact pin; 672. a support spring; 673. a first electrode ring; 674. a second electrode ring; 655. a wire; 656. performing branching; 680. a controller; 681. a connecting wire; 682. a spray head; 683. a soil-proof cover; 684. a heating pad; 685. a condensing tube; 657. a waterproof sleeve; 658. a ventilation hose; 70. a linkage assembly; 701. a positioning plate; 702. a fixed sleeve; 703. rotating the cylinder; 704. a push rod; 705. round table blocks; 706. a retraction spring; 707. a plug pin; 708. a linkage plate; 709. pushing and extruding a spring; 710. a limit groove; 711. limiting branched chains; 712. a limit clamp; 713. a turntable; 714. an auxiliary strip; 715. an annular seat; 716. a swinging rod; 717. and a limit ball.

Detailed Description

Embodiments of the invention are described in detail below with reference to fig. 1-13, but the invention can be implemented in a number of different ways, which are defined and covered by the claims.

The embodiment of the application discloses a contrast experiment device for microorganisms in soil, which is mainly applied to the process of contrast experiments on the types and the numbers of the microorganisms in the soil, and can manufacture different environments for soil samples in technical effect, so that the contrast experiments are carried out on the soil samples under different environments, whether the types and the numbers of the microorganisms in the soil samples are increased or decreased in different environments is judged according to experimental results, and the accuracy of the contrast experiments on the soil samples can be improved by converting the environments; particularly, in the environment conversion, the environment of the soil sample can be converted into a wet environment, a dry environment, a high-temperature environment and a low-temperature environment; further, this contrast experimental apparatus to microorganism in soil can also carry out many accuses and single accuse to the soil sample environmental conversion in a plurality of placing section of thick bamboo 2, and single accuse can carry out environmental conversion to the soil sample in a plurality of placing section of thick bamboo 2 respectively, and many accuses can carry out environmental conversion to the soil sample in a plurality of placing section of thick bamboo 2 simultaneously to can carry out the self-adaptation adjustment to the control of environmental conversion according to actual experiment demand, and the simple operation.

Example 1

Referring to fig. 1, 2 and 3, a contrast experiment device for microorganisms in soil comprises a bottom plate 1, wherein a plurality of placing cylinders 2 are installed above the bottom plate 1 at equal intervals along the length direction, positioning seats 21 are installed at the bottoms of the placing cylinders 2, clamping grooves are formed in the upper ends of the positioning seats 21, culture dishes 22 are clamped in the clamping grooves, the upper half parts of the clamping grooves are gradually inclined to one side far away from the center of the positioning seats 21, so that the culture dishes 22 are guided, and the culture dishes 22 can be smoothly placed in the clamping grooves; two telescopic cylinders 3 are symmetrically arranged between the lower end of the placing cylinder 2 and the upper end of the bottom plate 1, an upper cover 4 is clamped at the upper end of the placing cylinder 2, fixing columns 5 are arranged between the lower ends of four corners of the lower end of the upper cover 4 and the upper end of the bottom plate 1, and an environment conversion mechanism 6 positioned inside the placing cylinder 2 is arranged at the lower end of the upper cover 4.

In practical application, firstly, the telescopic cylinder 3 is started, the telescopic cylinder 3 drives the placing cylinder 2 to move downwards, secondly, the culture dishes 22 filled with soil samples in different regions are respectively placed in different placing cylinders 2, and then the telescopic cylinder 3 drives the placing cylinder 2 to move upwards and lean against the bottom of the upper cover 4, so that the upper cover 4 seals the soil samples in the placing cylinder 2, and the influence of external air on the environment of the soil samples is avoided; then, different environments are manufactured for the soil sample in the placing cylinder 2 through the environment manufacturing mechanism, microorganisms in the soil sample can be increased or reduced through the different environments, and finally, a comparison experiment is carried out on the soil sample under the different environments.

Referring to fig. 4, 5 and 6, in order to facilitate the comparison experiments of the types and numbers of the microorganisms in the soil sample under different environments, the environment where the soil sample is located needs to be converted, based on this, in this embodiment, an environment conversion mechanism 6 is provided, specifically, the environment conversion mechanism 6 includes a water tank 61, a middle part of the lower end of the upper cover 4 is provided with the water tank 61, a side wall of the water tank 61 is provided with a water inlet pipe 62 communicated with the interior thereof, an upper end of the water inlet pipe 62 extends upwards after passing through the upper cover 4, water can be injected into the water tank 61 through the water inlet pipe 62, a plurality of annularly distributed lower insertion barrels 63 and drain pipes 64 are uniformly arranged at the lower end of the water tank 61, the drain pipes 64 are inserted into the lower insertion barrels 63, and the upper end of the drain pipes 64 is communicated with the water tank 61, a conversion assembly 65 is installed at the upper end of the upper cover 4, the conversion assembly 65 includes a mounting block 651 disposed at the middle part of the upper end of the upper cover 4, and a linkage assembly 70 is installed between the mounting block 651 and the upper cover 4.

In a specific implementation process, the placing cylinder 2 moves upwards and abuts against the bottom of the upper cover 4, and the culture dish 22 can drive the soil sample to move synchronously, so that the lower inserting cylinder 63 is inserted into the soil sample, and then the environment inside the soil can be converted through the lower inserting cylinder 63 by the conversion assembly 65, so that whether the types and the numbers of microorganisms inside the soil sample are increased or reduced in different environments can be detected in the process of a comparison experiment.

Referring to fig. 6 and 7, in order to facilitate the transformation of the environment where the soil sample is located, in this embodiment, the transformation assembly 65 further includes an execution post 652, the installation block 651 is internally provided with an execution post 652 parallel to the length direction thereof, the installation block 651 is internally provided with a circular groove, an auxiliary branched chain 653 located inside the circular groove is installed between the execution post 652 and the installation block 651, three installation grooves are evenly spaced inside the installation block 651 along the clockwise direction of the execution post 652, a conducting wire 655 is provided in the installation groove through a triggering branched chain 654, one end of the conducting wire 655 far away from the triggering branched chain 654 sequentially passes through the upper cover 4 and the water tank 61 and then extends into the lower insertion barrel 63, the lower insertion barrel 63 is internally provided with an execution branched chain 656 connected with the conducting wire 655, the water tank 61 is internally provided with a waterproof sleeve 657 sleeved outside the conducting wires 655, and the conducting wire 655 can be protected through the waterproof sleeve 657, and potential hazards caused by the conducting wire 655 immersed in water are avoided.

Further, in this embodiment, a through hole communicating with the circular groove is formed in the mounting block 651 on one side where the mounting groove is not formed, a vent hose 658 communicating with the water tank 61 and the outside is disposed in the through hole, and the vent hose 658 is convenient for conveying external air into the water tank 61.

In a specific implementation process, the environment of the soil sample in the culture dish 22 can be converted through the cooperation among the execution post 652, the auxiliary branched chain 653, the trigger branched chain 654 and the execution branched chain 656, so that the soil sample in different environments can be conveniently subjected to a comparison experiment, and the accuracy of the soil sample comparison experiment can be improved through the conversion environment.

With continued reference to fig. 7, in order to be able to cooperate with the execution branch 656 to manufacture different environments for the soil sample, in this embodiment, the auxiliary branch 653 includes an annular cylinder 660, the middle part in the annular groove is rotationally connected with the annular cylinder 660, a plurality of key teeth 661 distributed in annular form are uniformly arranged on the inner side wall of the annular cylinder 660, a driving sleeve 662 is sleeved on one side of the outer wall of the execution post 652, and a plurality of key grooves 663 corresponding to the positions of the key teeth 661 are uniformly formed on the outer wall of the driving sleeve 662.

Further, in this embodiment, an annular groove 664 is provided on a side of the annular cylinder 660 away from the driving sleeve 662, and a flexible spring plate 665 is installed on any side of the outer wall of the annular cylinder 660, the flexible spring plate 665 always has a spring force recessed toward a side close to the annular groove 664, an annular plate 666 sleeved on the outer wall of the execution post 652 is rotatably connected to a side of the annular groove away from the driving sleeve 662, an annular pad 667 in sliding fit with the annular groove 664 is installed on a side of the annular plate 666 close to the annular cylinder 660, a chamfer is provided on an outer wall of a side of the annular pad 667 close to the annular groove 664, and the annular pad 667 is conveniently inserted into the annular groove 664 smoothly through the chamfer.

In the initial state, the active sleeve 662 is positioned at the side of the annular cylinder 660 away from the annular plate 666, the annular pad 667 is inserted into the annular groove 664, and thus the flexible spring plate 665 is supported by the annular pad 667, at this time, the air hose 658 is pressed closed and does not allow external air to enter the water tank 61, and thus water in the water tank 61 is subjected to negative pressure and does not flow out of the drain pipe 64.

As shown in fig. 8, since the triggering branch 654 is required when different environments are manufactured in the soil sample, in this embodiment, the triggering branch 654 is provided, specifically, the triggering branch 654 includes a fixed barrel 670, a fixed barrel 670 is provided in a mounting groove, an inner wall of the fixed barrel 670 is slidably connected with a supporting pin 671 through a displacement plate, a wire 655 is connected with an upper end of the displacement plate, a supporting spring 672 is installed between the upper end of the displacement plate and an inner top wall of the fixed barrel 670, one end of the supporting pin 671 far from the displacement plate passes through the fixed barrel 670 and the mounting groove and slidably supports against the annular barrel 660, the supporting spring 672 always applies a supporting force directed to one side of the annular barrel 660 to the displacement plate, so that the displacement plate drives the supporting pin 671 to slidably support against the annular barrel 660 after passing through the fixed barrel 670 and the mounting groove, a first electrode ring 673 sleeved outside the supporting pin 673 is provided at a lower end of the displacement plate, and a second electrode ring 674 matched with the first electrode ring 673 is installed at an inner bottom wall of the fixed barrel 670.

In the initial state, the abutting pin 671 abuts against the outer wall of the annular barrel 660 and drives the first electrode ring 673 and the second electrode ring 674 to be separated through the displacement plate.

In a specific implementation process, the execution post 652 drives the annular plate 666 and the annular pad 667 to move to one side far away from the annular barrel 660, so that the annular pad 667 is pulled out of the annular groove 664, and the flexible elastic sheet 665 is recessed under the self elastic action; at this time, the actuating post 652 drives the driving sleeve 662 to be inserted into the inner side of the annular cylinder 660, so that the key teeth 661 are slidably inserted into the key grooves 663, and the actuating post 652 can drive the annular cylinder 660 to rotate through the cooperation between the key teeth 661 and the key grooves 663.

When the concave flexible spring plate 665 rotates to the position of the ventilation hose 658, the ventilation hose 658 is not squeezed any more, so that external air enters the water tank 61 through the ventilation hose 658, and the negative pressure state of the water tank 61 is relieved, so that water in the water tank 61 flows out through the water drain pipe 64, and water spraying of the branched chains 656 to the soil sample in the culture dish 22 is performed cooperatively, and the moist state of the soil sample is simulated; when the concave flexible spring plate 665 rotates to the position of the abutting pin 671, the abutting pin 671 drives the first electrode ring 673 to contact with the second electrode ring 674 under the action of the supporting spring 672, so that the first electrode ring 673 and the second electrode ring 674 cooperate with the wire 655 to execute the branched chain 656 to transmit signals, so as to facilitate the conversion of the environment where the soil sample is located, and further, the soil samples in different environments can be subjected to comparison experiments, further, the experimental effect is enhanced, and the experimental diversity is improved.

Referring to fig. 9 and 10, in order to facilitate the conversion of different environments of the soil sample by matching the auxiliary branched chain 653 and the trigger branched chain 654, in this embodiment, the execution branched chain 656 includes a controller 680, the lower ends of the plurality of wires 655 are commonly connected with the controller 680, a plurality of connecting wires 681 which are uniformly distributed and extend to the inside of the lower insert barrel 63 are uniformly arranged at the lower end of the controller 680, a plurality of spray heads 682 which are connected with the connecting wires 681 are uniformly arranged at equal intervals from top to bottom and circumferentially on the outer wall of the drain pipe 64, a plurality of through holes corresponding to the positions of the spray heads 682 are uniformly formed on the outer wall of the lower insert barrel 63, a soil protection cover 683 is arranged on the inner wall of each through hole, and the soil sample can be blocked by the soil protection cover 683 to prevent the spray heads 682 from blocking.

Further, in the present embodiment, a plurality of annular accommodating grooves are uniformly formed in the inner wall of the lower insertion tube 63, a heating pad 684 connected with a connecting line 681 is disposed in the accommodating grooves, a plurality of condensation pipes 685 corresponding to the heating pad 684 are uniformly mounted on the outer wall of the lower insertion tube 63, and the condensation pipes 685 are connected with the connecting line 681.

It should be noted that, the three sets of the first electrode ring 673 and the second electrode ring 674 in the mounting groove correspond to the heating pad 684 and the condensation duct 685 in the lower socket 63, respectively.

In a specific implementation process, when one group of first electrode rings 673 and second electrode rings 674 in the installation groove are in contact, signals are transmitted to the controller 680 through the wires 655, and the controller 680 controls the heating pad 684 to dry the soil in the culture dish 22 at a low temperature through the connecting wire 681, so that the soil sample is in a dry state; when the other group of first electrode rings 673 and second electrode rings 674 are contacted, the controller 680 controls the heating pad 684 to heat the soil in the culture dish 22 through the connecting wire 681, so that the temperature inside the soil sample is higher; when the last set of first electrode rings 673 and second electrode rings 674 are in contact, the controller 680 controls the condensing tube 685 to cool the soil in the culture dish 22 through the connecting line 681, so that the temperature inside the soil sample is reduced.

Therefore, the first electrode ring 673 and the second electrode ring 674 are controlled to be contacted by the rotating annular cylinder 660, so that the environment state of the soil sample in the culture dish 22 is converted, and the environment state is specifically divided into a humid environment, a dry environment, a high-temperature environment and a low-temperature environment, so that the soil samples in different environments are subjected to comparison experiments, and whether the types and the numbers of microorganisms in the soil samples are increased or decreased in different environments is judged according to the experimental results.

Example two

Referring to fig. 11 and 12, on the basis of the first embodiment, since a plurality of placing drums 2 may be placed with soil samples of different areas or soil samples of the same area, the different soil samples need to be subjected to comparison experiments in different environments or under the same environment; based on this, it is necessary to perform synchronous environmental conversion or perform environmental conversion one by one on the soil sample inside the placing cylinder 2, specifically, the linkage assembly 70 includes a positioning plate 701, two positioning plates 701 are symmetrically disposed at the upper end of the upper cover 4 along the mounting block 651, two ends of the execution post 652 pass through the mounting block 651 and are rotationally connected with the positioning plate 701, the ends of two adjacent execution posts 652 are respectively mounted with a fixed sleeve 702 and a rotating cylinder 703, the rotating cylinder 703 is rotationally abutted inside the fixed sleeve 702, a yielding groove is formed inside the rotating cylinder 703, a push rod 704 is slidingly disposed inside the execution post 652, a round table block 705 slidingly abutted inside the yielding groove is mounted at one end of the push rod 704, a shrinkage spring 706 sleeved outside the push rod 704 is mounted between the round table block 705 and the yielding groove, and the push rod 704 in the same yielding groove is connected with the opposite round table block 705.

Further, in this embodiment, a plurality of displacement slots which are distributed annularly and are communicated with the abdication slots are uniformly formed in the rotary cylinder 703, a plug pin 707 is slidably connected in the displacement slots, a linkage plate 708 is sleeved on the outer wall of the plug pin 707, a pushing spring 709 is arranged between one side of the linkage plate 708 away from the abdication slots and the inner wall of the displacement slots, one end of the plug pin 707, which is close to the abdication slots, is in sliding contact with the round table block 705, a plurality of limit slots 710 corresponding to the positions of the displacement slots are uniformly formed in the inner side wall of the fixed sleeve 702, and a limit branched chain 711 is installed between the execution column 652 and the positioning plate 701.

Under the initial condition, the shrinkage spring 706 always applies shrinkage force to the round platform block 705, so that the round platform block 705 is shrunk to the side wall of the yielding groove in the initial condition, the pushing spring 709 always applies pushing force pointing to one side of the yielding groove to the linkage plate 708 and the plug pin 707, at the moment, the plug pin 707 is not contacted with the round platform block 705, the end part of the plug pin does not extend out of the displacement groove, and when the execution column 652 on any installation block 651 is rotated, other execution columns 652 cannot be driven to synchronously rotate, at the moment, single control can be formed, and soil samples in different placing cylinders 2 can be respectively subjected to environment conversion.

In a specific implementation process, the push rod 704 is pushed, the push rod 704 drives the round platform block 705 to synchronously move, the round platform block 705 applies a pushing force to the plug pin 707, so that the plug pin 707 extends out of the displacement groove and is inserted into the limit groove 710, the fixed sleeve 702 and the rotary cylinder 703 can be locked through matching between the plug pin 707 and the limit groove 710, and therefore when any execution column 652 is rotated, other execution columns 652 can be driven to synchronously rotate, multiple control can be formed at the moment, environment conversion can be conducted on soil samples in a plurality of placing cylinders 2 at the same time, self-adaptive adjustment can be conducted on the control of the environment conversion according to actual experiment requirements, and operation is convenient.

When the single control is needed, the round platform block 705 is driven to reset by the push rod 704, the plug pin 707 does not bear the pushing force of the round platform block 705 any more, and is retracted into the displacement groove under the action of the pushing spring 709, so that the locking effect on the fixed sleeve 702 and the rotary cylinder 703 is relieved.

Referring to fig. 12, in order to avoid the random change of the environment of the soil sample caused by the random rotation of the execution post 652, the execution post 652 needs to be limited, specifically, the limiting branched chain 711 further comprises an annular seat 715, the outer wall of the execution post 652 is symmetrically sleeved with two annular seats 715 in a sliding manner along the positioning plate 701, the annular seats 715 are rotationally connected with the positioning plate 701, a plurality of annularly distributed swinging rods 716 are uniformly hinged to the outer wall of the annular seat 715 through torsion springs, a torsion force pointing to one side of the positioning plate 701 is always applied to the swinging rods 716 through the arrangement of the torsion springs, a limiting ball 717 is arranged on one side of the swinging rods 716 close to the positioning plate 701, and a plurality of grooves which are in sliding contact with the limiting ball 717 are formed in the outer wall of the positioning plate 701.

In a specific implementation process, the annular seat 715, the swing rod 716 and the limiting ball 717 are driven to move circumferentially in the rotation process of the execution post 652, after the rotation of the execution post 652 is completed, the limiting ball 717 on the side wall of the swing rod 716 abuts against the inside of the groove, so that the execution can be limited through the matching between the limiting ball 717 and the groove, the execution post 652 is prevented from rotating randomly, the flexible elastic sheet 665 is prevented from moving away from the abutting pin 671, and the soil sample environment inside the placement barrel 2 is further closed.

Referring to fig. 13, in order to prevent the fixed sleeve 702 and the rotating cylinder 703 from unlocking due to random rebound of the push rod 704, corresponding limitation needs to be performed on the push rod 704, specifically, the limitation branched chain 711 includes a limitation card 712, a plurality of limitation cards 712 distributed in a ring shape are uniformly installed after the end of the execution column 652 located above the outermost upper cover 4 passes through the positioning plate 701, the limitation card 712 is in an L-shaped structure, a folded edge is provided on a horizontal segment of the limitation card 712, a turntable 713 is rotatably provided on the end of the push rod 704 inside the execution column 652, and a plurality of auxiliary strips 714 corresponding to the positions of the limitation cards 712 are uniformly provided on the outer wall of the turntable 713.

In a specific implementation process, the push rod 704 is pushed by the rotary table 713, at this time, the auxiliary strips 714 on the outer wall of the rotary table 713 are staggered with the limit cards 712, then the rotary table 713 rotates, and the rotary table 713 drives the auxiliary strips 714 to be clamped in the limit cards 712, so that the push rod 704 can be limited by matching between the auxiliary strips 714 and the limit cards 712, the push rod 704 and the round table block 705 are prevented from rebounding under the action of the shrinkage spring 706, the plug pins 707 are pulled out of the limit grooves 710, and the fixed sleeve 702 and the rotary cylinder 703 are ensured to be always kept in a locking state.

When in operation, the device comprises: the first step: firstly, the placing cylinder 2 is driven to move downwards through the telescopic cylinder 3, secondly, the culture dishes 22 filled with soil samples are respectively placed in different placing cylinders 2, and then the telescopic cylinder 3 drives the placing cylinder 2 to move upwards and lean against the bottom of the upper cover 4, so that the upper cover 4 seals the soil samples in the placing cylinder 2, and the influence of outside air on the environment of the soil samples is avoided.

The placing cylinder 2 moves upwards and abuts against the bottom of the upper cover 4, and the culture dish 22 can drive the soil sample to move synchronously, so that the lower inserting cylinder 63 is inserted into the soil sample.

And a second step of: the annular plate 666 and the annular pad 667 are driven by the execution post 652 to move to the side far away from the annular barrel 660, so that the annular pad 667 is pulled out of the annular groove 664, and the flexible spring plate 665 is recessed under the self elastic action; at this time, the actuating post 652 drives the driving sleeve 662 to be inserted into the inner side of the annular cylinder 660, so that the key teeth 661 are slidably inserted into the key grooves 663, and the actuating post 652 can drive the annular cylinder 660 to rotate.

And a third step of: when the concave flexible spring plate 665 rotates to the position of the ventilation hose 658, the ventilation hose 658 is not squeezed any more, so that external air enters the water tank 61 through the ventilation hose 658 and the negative pressure state of the water tank 61 is released, so that water in the water tank 61 flows out through the water drain pipe 64, and the water spray of the branched chain 656 to the soil sample in the culture dish 22 is matched, so that the moist state of the soil sample is simulated.

When the concave flexible spring plate 665 rotates to the position of the abutting pin 671, the abutting pin 671 drives the first electrode ring 673 to contact with the second electrode ring 674 under the action of the supporting spring 672; when one of the first electrode rings 673 and the second electrode ring 674 are in contact, a signal is transmitted to the controller 680 through the wire 655, and the controller 680 controls the heating pad 684 to dry the soil in the culture dish 22 at a low temperature, so that the soil sample is in a dry state; when the other group of first electrode rings 673 and second electrode rings 674 are contacted, the controller 680 controls the heating pad 684 to heat the soil in the culture dish 22, and the temperature inside the soil sample is higher; when the last group of first electrode rings 673 and second electrode rings 674 are contacted, the controller 680 controls the condensing tube 685 to cool the soil in the culture dish 22, and the temperature inside the soil sample is reduced; so as to be convenient for converting the environment where the soil sample is located, thereby being capable of carrying out contrast experiments on the soil samples in different environments and further enhancing the experimental effect.

Fourth step: the round platform block 705 is driven by the push rod 704 to synchronously move, and the round platform block 705 applies pushing force to the plug pin 707, so that the plug pin 707 extends out of the displacement groove, is inserted into the limit groove 710 and locks between the fixed sleeve 702 and the rotating cylinder 703, and when any one execution post 652 is rotated, other execution posts 652 can be driven to synchronously rotate, so that the soil samples in a plurality of placing cylinders 2 can be simultaneously subjected to environment conversion.

When the conversion is needed to be single control, the round platform block 705 is driven to reset through the push rod 704, the plug pin 707 does not bear the pushing force of the round platform block 705 any more and is retracted into the displacement groove under the action of the pushing spring 709, the locking effect on the fixed sleeve 702 and the rotary cylinder 703 is relieved, at the moment, the execution column 652 on any mounting block 651 cannot be driven to synchronously rotate when the execution column 652 on the other mounting block 651 is rotated, at the moment, single control can be formed, and the soil samples in different placing cylinders 2 can be respectively subjected to environment conversion.

Fifth step: after pushing the push rod 704, the turntable 713 is rotated, and the turntable 713 drives the auxiliary bar 714 to be clamped in the limiting clamp 712, so that the push rod 704 can be limited through the matching between the auxiliary bar 714 and the limiting clamp 712, and the push rod 704 and the round table block 705 are prevented from rebounding under the action of the contraction spring 706 to cause the plug pin 707 to be pulled out of the limiting groove 710.

After the rotation of the execution post 652 is completed, the limiting ball 717 on the side wall of the swinging rod 716 abuts against the groove, so that the execution can be limited through the cooperation between the limiting ball 717 and the groove, and the flexible elastic sheet 665 is prevented from being moved away from the abutting pin 671 due to the random rotation of the execution post 652, so that the soil sample environment inside the placing cylinder 2 is closed.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.

Claims (4)

1. The utility model provides a contrast experimental apparatus to microorganism in soil, includes bottom plate (1), and a plurality of section of thick bamboo (2) are placed along length direction equidistant installing in bottom plate (1) top, and the symmetry is provided with two telescopic cylinder (3) between section of thick bamboo (2) lower extreme and bottom plate (1) upper end, and section of thick bamboo (2) upper end joint has upper cover (4) of placing, all installs fixed column (5) between four corners lower extreme of upper cover (4) lower extreme and bottom plate (1) upper end, its characterized in that: the upper cover (4) lower extreme is provided with and is located the inside environment conversion mechanism (6) of a section of thick bamboo (2) of placing, wherein:

the environment conversion mechanism (6) comprises a water tank (61), the middle part of the lower end of an upper cover (4) is provided with the water tank (61), the side wall of the water tank (61) is provided with a water inlet pipe (62) communicated with the inside of the water tank, the upper end of the water inlet pipe (62) penetrates through the upper cover (4) and then extends upwards, the lower end of the water tank (61) is uniformly provided with a plurality of annularly distributed lower inserting cylinders (63) and a plurality of water discharging pipes (64), the water discharging pipes (64) are inserted into the lower inserting cylinders (63), the upper end of the water discharging pipes (64) are communicated with the water tank (61), the upper end of the upper cover (4) is provided with a conversion assembly (65), the conversion assembly (65) comprises a mounting block (651) arranged in the middle of the upper end of the upper cover (4), a linkage assembly (70) is arranged between the mounting block (651) and the upper cover (4), the linkage assembly (70) comprises positioning plates (701), and the upper end of the upper cover (4) is symmetrically provided with two positioning plates (701) along the mounting block (651).

The conversion assembly (65) further comprises an execution column (652), the inside of the installation block (651) is provided with an execution column (652) which is parallel to the length direction of the execution column, a round groove is formed in the installation block (651), an auxiliary branched chain (653) which is positioned in the round groove is arranged between the execution column (652) and the installation block (651), three installation grooves are formed in the installation block (651) at equal intervals along the clockwise direction of the execution column (652), wires (655) are arranged in the installation grooves through triggering branched chains (654), one end, far away from the triggering branched chains (654), of each wire (655) sequentially penetrates through an upper cover (4) and a water tank (61) and then extends into the lower insertion cylinder (63), an execution branched chain (656) which is connected with the wires (655) is installed in the lower insertion cylinder (63), and waterproof sleeves (657) which are sleeved outside the wires (655) are installed in the water tank (61).

One side of the installation block (651) which is not provided with an installation groove is provided with a through hole communicated with the circular groove in a penetrating way, and the through hole is internally provided with a ventilation hose (658) communicated with the water tank (61) and the outside;

the auxiliary branched chain (653) comprises an annular cylinder (660), the middle part in the annular groove is rotationally connected with the annular cylinder (660), a plurality of annular distributed key teeth (661) are uniformly arranged on the inner side wall of the annular cylinder (660), a driving sleeve (662) is sleeved on one side of the outer wall of the execution column (652), and a plurality of key grooves (663) corresponding to the key teeth (661) in position are uniformly formed in the outer wall of the driving sleeve (662);

an annular groove (664) is formed in one side, far away from the driving sleeve (662), of the annular cylinder (660), a flexible spring piece (665) is arranged on any side of the outer wall of the annular cylinder (660), an annular plate (666) sleeved on the outer wall of the execution column (652) is rotatably connected to one side, far away from the driving sleeve (662), of the annular groove, an annular pad (667) in sliding fit with the annular groove (664) is arranged on one side, close to the annular cylinder (660), of the annular plate (666), and a chamfer is formed in the outer wall, close to the annular groove (664);

the trigger branched chain (654) comprises a fixed barrel (670), a fixed barrel (670) is arranged in a mounting groove, an interference pin (671) is slidably connected to the inner wall of the fixed barrel (670) through a displacement plate, a lead (655) is connected with the upper end of the displacement plate, a supporting spring (672) is arranged between the upper end of the displacement plate and the inner top wall of the fixed barrel (670), one end, far away from the displacement plate, of the interference pin (671) penetrates through the fixed barrel (670) and the mounting groove and slidably interferes with an annular barrel (660), a first electrode ring (673) sleeved outside the interference pin (671) is arranged at the lower end of the displacement plate, and a second electrode ring (674) matched with the first electrode ring (673) is arranged on the inner bottom wall of the fixed barrel (670);

the execution branched chain (656) comprises a controller (680), wherein the lower ends of a plurality of wires (655) are commonly connected with the controller (680), a plurality of connecting wires (681) which are uniformly distributed and extend to the inside of a lower insertion barrel (63) are uniformly arranged at the lower end of the controller (680), a plurality of spray heads (682) which are connected with the connecting wires (681) are uniformly arranged on the outer wall of a drain pipe (64) from top to bottom at equal intervals in the circumferential direction, a plurality of through holes corresponding to the positions of the spray heads (682) are uniformly formed on the outer wall of the lower insertion barrel (63), and soil-proof covers (683) are arranged on the inner walls of the through holes;

a plurality of annularly-distributed accommodating grooves are uniformly formed in the inner wall of the lower inserting cylinder (63), heating pads (684) connected with connecting wires (681) are arranged in the accommodating grooves, a plurality of condensing pipes (685) corresponding to the positions of the heating pads (684) are uniformly arranged on the outer wall of the lower inserting cylinder (63), and the condensing pipes (685) are connected with the connecting wires (681);

the linkage assembly (70) further comprises a fixed sleeve (702), two ends of the execution column (652) are rotatably connected with the positioning plate (701) after passing through the installation blocks (651), the ends of two adjacent execution columns (652) are respectively provided with the fixed sleeve (702) and a rotary cylinder (703), the rotary cylinders (703) are rotatably abutted to the inside of the fixed sleeve (702), the inside of the rotary cylinder (703) is provided with a yielding groove, the inside of the execution column (652) is slidably penetrated with a push rod (704), one end of the push rod (704) is provided with a round platform block (705) which is slidably abutted to the yielding groove, a contraction spring (706) sleeved outside the push rod (704) is arranged between the round platform block (705) and the yielding groove, and the push rod (704) in the same yielding groove is connected with the opposite round platform block (705);

a plurality of annular distribution and the displacement groove that is linked together with the groove of stepping down have evenly been seted up to rotation cylinder (703), sliding connection has spliced pin (707) in the displacement groove, spliced pin (707) outer wall cover is equipped with linkage board (708), be provided with between one side that the groove was stepped down was kept away from to linkage board (708) and the displacement inslot wall and push spring (709), the one end that spliced pin (707) is close to the groove of stepping down and round platform piece (705) slip butt, a plurality of spacing grooves (710) corresponding with the displacement groove position have evenly been seted up to fixed sleeve (702) inside wall, install spacing branch (711) between executive column (652) and locating plate (701).

2. A comparative experimental device for microorganisms in soil according to claim 1, wherein: limiting branch chain (711) still includes annular seat (715), and execution post (652) outer wall is equipped with two annular seats (715) along locating plate (701) symmetry slip cover, rotates between annular seat (715) and locating plate (701) and is connected, and annular seat (715) outer wall has a plurality of annular swinging arms (716) of distributing through the torsional spring evenly articulated, and one side that swinging arms (716) are close to locating plate (701) is provided with spacing ball (717), and a plurality of recess that contradict with spacing ball (717) slip are seted up to locating plate (701) outer wall.

3. A comparative experimental device for microorganisms in soil according to claim 1, wherein: spacing branch chain (711) include spacing card (712), are located behind execution post (652) tip of outside upper cover (4) top and pass locating plate (701) evenly install spacing card (712) that a plurality of annular distribute, spacing card (712) are L type structure, and the horizontal segment of spacing card (712) is provided with the hem, and the inside push rod (704) tip rotation of this execution post (652) is provided with carousel (713), carousel (713) outer wall evenly is provided with a plurality of supplementary strips (714) corresponding with spacing card (712) position.

4. A comparative experimental device for microorganisms in soil according to claim 1, wherein: the bottom of the placing barrel (2) is provided with a positioning seat (21), the upper end of the positioning seat (21) is provided with a clamping groove, a culture dish (22) is clamped in the clamping groove, and the upper half part of the clamping groove is gradually inclined to one side far away from the center of the positioning seat (21).