CN117106941A - 一种鉴定香菇品种的mnp标记法的开发及应用 - Google Patents
一种鉴定香菇品种的mnp标记法的开发及应用 Download PDFInfo
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Abstract
本发明公开了一种鉴定香菇品种的MNP标记法的开发及应用。本发明基于香菇187个菌株基因组重测序数据,筛选得到501个广泛MNP标记位点;通过相关引物设计和对78份香菇商业品种的多重扩增、MNP‑Seq测序,构建了香菇的MNP标记位点数据库。在此基础上进一步筛选187个具有100%检测率的MNP标记位点作为核心MNP标记位点,利用240个菌株的核心位点序列构建系统发育图谱,计算不同谱系间和品种间遗传相似度,据此判断两个品种为不同品种还是相同品种,为不同谱系还是相同谱系。本发明具有重要的应用价值。
Description
技术领域
本发明属于生物技术领域,具体涉及一种鉴定香菇品种的MNP标记法的开发及应用。
背景技术
香菇[Lentinula edodes(Berk.)pegler.],是一类在世界范围内广泛种植的食用菌,分类学上隶属于担子菌(Basidiomycota)、蘑菇纲(Agaricomycetes)、蘑菇目(Agaricales)、口蘑科(Tricholomataceae)、香菇属(Lentinula)。香菇一直是我国年产量最大的食用菌品类,其年产量占食用菌年总产量的27.15%。中国是最早栽培香菇的国家,栽培历史悠久,品种资源丰富。然而随着品种选育、种质交流和引种栽培等工作的推进,同一菌株名称不同或不同菌株名称相同的现象普遍存在。根据2008年—2015年全国各单位选育菌株情况统计结果,不同名称的香菇栽培菌株由150个增长至500个,其中27个是国家审定品种,37个是地方审定(鉴定、备案)品种。这些调查结果反映了香菇品种数量众多,增加了品种管理的难度;而且栽培品种名称混乱,种质来源不明确,这导致侵权行为普遍存在,育种人权益得不到保障,严重阻碍市场发展。此外,文献研究表明,我国香菇的栽培种质资源的遗传多样性低。因为我国的香菇栽培菌株大多从数量有限的优良品系中衍生出来,以致遗传基础越来越狭窄,这给品种鉴别带来困难。
使用传统的形态学、理化和同工酶等方法对香菇品种进行鉴定,结果表明,亲缘关系密切的类群无法被区分开,且鉴定耗时较长。分子标记具有许多优势,例如:分子标记数量巨大,覆盖生物体的全部基因组;分子标记中存在更高的多态性,可以揭示变异和等位基因;分子标记是共显性的,可以区分纯合和杂合基因型;分子标记是“中性”的,它们对表型没有影响,没有上位效应,并且不受环境因素和发育阶段的影响。此外,分子标记还具有简单、快速、实验可重复性好等优点,近年来被广泛用于植物品种的鉴定、登记、种子纯度检测及真实性检验,具有高准确性、高可靠性和低成本等特点。为了解决国内香菇品种繁多杂乱的问题,需要找到一种高效精确的分子标记方法。目前市面上已存在一些分子标记,如ISSR、RAPD、SSR和SNP等,但依然存在工作量大、精确性和重复性不足等问题,品种间难以准确的区分,极大影响了产业发展和种质创新进程。
多核苷酸多态性(Multiple Nucleotide Polymorphism,MNP)标记法是在单核苷酸多态性标记的基础上发展起来的,为了在每个靶位点检测尽可能多的SNP变异,设计了一种在单个扩增子中检测多个SNP的方法,其原理是在基因组内小于300bp的碱基序列中存在多个SNP标记,不同SNP标记的等位基因型组合可以区分不同的个体DNA。MNP标记法有几大优势:一、有丰富的等位基因。MNP标记的等位基因数高达2n(n:MNP标记中SNP的个数),远多于SNP标记。MNP标记具有标记数量丰富、品种区分力强的特点。二、MNP检测准确率高。筛选MNP候选区段时是避开了易出错的序列,如SSR和连续的SNP位点。基于高通量测序的MNP标记检测克服了凝胶电泳中SSR扩增长度的不确定性和SNP标记的微阵列杂交噪声。三、MNP检测可以定量。利用第二代高通量测序技术,在同一MNP标记位点重复检测数百个测序片段,可以量化等位基因比例和多倍体基因剂量等。四、MNP检测效率高。一次PCR反应就可以完成数百个MNP标记位点的扩增;一次高通量测序可同时检测数百个样本的数千个MNP标记,效率极高。
MNP标记能够准确检测侵权植物品种和保护植物品种知识产权,并在种质指纹图谱和分子辅助选择(MAS)方面具有很大的潜力,与表型性状相关的标记已被用于在植物育种计划中选择理想性状。现已开发出水稻、大豆、油菜、茄子等的MNP标记引物组合,应用于遗传学和育种研究。然而,MNP标记法在食用菌的品种鉴别中尚未得到应用。
发明内容
本发明的目的是鉴定香菇品种。
本发明首先保护引物组合甲,可包括引物1—引物1002;所述引物1—引物1002的核苷酸序列依次如SEQ ID NO:1-SEQ ID NO:1002所示。
所述引物组合甲具体可由引物1—引物1002组成。
本发明还保护引物组合乙。所述引物组合乙具体可由引物5、引物6、引物7、引物8、引物11、引物12、引物15、引物16、引物19、引物20、引物21、引物22、引物25、引物26、引物35、引物36、引物45、引物46、引物49、引物50、引物51、引物52、引物67、引物68、引物73、引物74、引物87、引物88、引物97、引物98、引物99、引物100、引物105、引物106、引物115、引物116、引物117、引物118、引物131、引物132、引物139、引物140、引物145、引物146、引物147、引物148、引物165、引物166、引物171、引物172、引物173、引物174、引物177、引物178、引物179、引物180、引物181、引物182、引物183、引物184、引物187、引物188、引物195、引物196、引物197、引物198、引物205、引物206、引物213、引物214、引物215、引物216、引物227、引物228、引物237、引物238、引物239、引物240、引物243、引物244、引物253、引物254、引物255、引物256、引物257、引物258、引物263、引物264、引物265、引物266、引物267、引物268、引物271、引物272、引物273、引物274、引物281、引物282、引物283、引物284、引物287、引物288、引物295、引物296、引物299、引物300、引物301、引物302、引物303、引物304、引物313、引物314、引物315、引物316、引物321、引物322、引物339、引物340、引物347、引物348、引物355、引物356、引物375、引物376、引物379、引物380、引物381、引物382、引物383、引物384、引物393、引物394、引物395、引物396、引物397、引物398、引物399、引物400、引物407、引物408、引物411、引物412、引物423、引物424、引物429、引物430、引物435、引物436、引物437、引物438、引物441、引物442、引物451、引物452、引物463、引物464、引物465、引物466、引物473、引物474、引物475、引物476、引物477、引物478、引物479、引物480、引物491、引物492、引物493、引物494、引物495、引物496、引物497、引物498、引物501、引物502、引物507、引物508、引物511、引物512、引物513、引物514、引物515、引物516、引物517、引物518、引物519、引物520、引物523、引物524、引物529、引物530、引物531、引物532、引物533、引物534、引物535、引物536、引物539、引物540、引物541、引物542、引物549、引物550、引物553、引物554、引物557、引物558、引物559、引物560、引物563、引物564、引物567、引物568、引物571、引物572、引物573、引物574、引物579、引物580、引物583、引物584、引物605、引物606、引物607、引物608、引物611、引物612、引物615、引物616、引物621、引物622、引物623、引物624、引物627、引物628、引物629、引物630、引物635、引物636、引物643、引物644、引物649、引物650、引物661、引物662、引物665、引物666、引物673、引物674、引物685、引物686、引物687、引物688、引物691、引物692、引物693、引物694、引物695、引物696、引物703、引物704、引物705、引物706、引物713、引物714、引物717、引物718、引物723、引物724、引物725、引物726、引物727、引物728、引物729、引物730、引物731、引物732、引物739、引物740、引物741、引物742、引物743、引物744、引物747、引物748、引物749、引物750、引物751、引物752、引物753、引物754、引物755、引物756、引物767、引物768、引物769、引物770、引物775、引物776、引物779、引物780、引物807、引物808、引物813、引物814、引物823、引物824、引物831、引物832、引物833、引物834、引物835、引物836、引物843、引物844、引物845、引物846、引物851、引物852、引物857、引物858、引物861、引物862、引物871、引物872、引物875、引物876、引物885、引物886、引物893、引物894、引物909、引物910、引物911、引物912、引物913、引物914、引物915、引物916、引物923、引物924、引物927、引物928、引物929、引物930、引物933、引物934、引物937、引物938、引物939、引物940、引物947、引物948、引物951、引物952、引物953、引物954、引物959、引物960、引物961、引物962、引物965、引物966、引物967、引物968、引物971、引物972、引物977、引物978、引物989、引物990、引物999和引物1000组成;
引物5、引物6、引物7、引物8、引物11、引物12、引物15、引物16、引物19、引物20、引物21、引物22、引物25、引物26、引物35、引物36、引物45、引物46、引物49、引物50、引物51、引物52、引物67、引物68、引物73、引物74、引物87、引物88、引物97、引物98、引物99、引物100、引物105、引物106、引物115、引物116、引物117、引物118、引物131、引物132、引物139、引物140、引物145、引物146、引物147、引物148、引物165、引物166、引物171、引物172、引物173、引物174、引物177、引物178、引物179、引物180、引物181、引物182、引物183、引物184、引物187、引物188、引物195、引物196、引物197、引物198、引物205、引物206、引物213、引物214、引物215、引物216、引物227、引物228、引物237、引物238、引物239、引物240、引物243、引物244、引物253、引物254、引物255、引物256、引物257、引物258、引物263、引物264、引物265、引物266、引物267、引物268、引物271、引物272、引物273、引物274、引物281、引物282、引物283、引物284、引物287、引物288、引物295、引物296、引物299、引物300、引物301、引物302、引物303、引物304、引物313、引物314、引物315、引物316、引物321、引物322、引物339、引物340、引物347、引物348、引物355、引物356、引物375、引物376、引物379、引物380、引物381、引物382、引物383、引物384、引物393、引物394、引物395、引物396、引物397、引物398、引物399、引物400、引物407、引物408、引物411、引物412、引物423、引物424、引物429、引物430、引物435、引物436、引物437、引物438、引物441、引物442、引物451、引物452、引物463、引物464、引物465、引物466、引物473、引物474、引物475、引物476、引物477、引物478、引物479、引物480、引物491、引物492、引物493、引物494、引物495、引物496、引物497、引物498、引物501、引物502、引物507、引物508、引物511、引物512、引物513、引物514、引物515、引物516、引物517、引物518、引物519、引物520、引物523、引物524、引物529、引物530、引物531、引物532、引物533、引物534、引物535、引物536、引物539、引物540、引物541、引物542、引物549、引物550、引物553、引物554、引物557、引物558、引物559、引物560、引物563、引物564、引物567、引物568、引物571、引物572、引物573、引物574、引物579、引物580、引物583、引物584、引物605、引物606、引物607、引物608、引物611、引物612、引物615、引物616、引物621、引物622、引物623、引物624、引物627、引物628、引物629、引物630、引物635、引物636、引物643、引物644、引物649、引物650、引物661、引物662、引物665、引物666、引物673、引物674、引物685、引物686、引物687、引物688、引物691、引物692、引物693、引物694、引物695、引物696、引物703、引物704、引物705、引物706、引物713、引物714、引物717、引物718、引物723、引物724、引物725、引物726、引物727、引物728、引物729、引物730、引物731、引物732、引物739、引物740、引物741、引物742、引物743、引物744、引物747、引物748、引物749、引物750、引物751、引物752、引物753、引物754、引物755、引物756、引物767、引物768、引物769、引物770、引物775、引物776、引物779、引物780、引物807、引物808、引物813、引物814、引物823、引物824、引物831、引物832、引物833、引物834、引物835、引物836、引物843、引物844、引物845、引物846、引物851、引物852、引物857、引物858、引物861、引物862、引物871、引物872、引物875、引物876、引物885、引物886、引物893、引物894、引物909、引物910、引物911、引物912、引物913、引物914、引物915、引物916、引物923、引物924、引物927、引物928、引物929、引物930、引物933、引物934、引物937、引物938、引物939、引物940、引物947、引物948、引物951、引物952、引物953、引物954、引物959、引物960、引物961、引物962、引物965、引物966、引物967、引物968、引物971、引物972、引物977、引物978、引物989、引物990、引物999和引物1000的核苷酸序列依次可如SEQ ID NO:5、SEQ ID NO:6、SEQ ID NO:7、SEQ ID NO:8、SEQ IDNO:11、SEQ ID NO:12、SEQ ID NO:15、SEQ ID NO:16、SEQ ID NO:19、SEQ ID NO:20、SEQ IDNO:21、SEQ ID NO:22、SEQ ID NO:25、SEQ ID NO:26、SEQ ID NO:35、SEQ ID NO:36、SEQ IDNO:45、SEQ ID NO:46、SEQ ID NO:49、SEQ ID NO:50、SEQ ID NO:51、SEQ ID NO:52、SEQ IDNO:67、SEQ ID NO:68、SEQ ID NO:73、SEQ ID NO:74、SEQ ID NO:87、SEQ ID NO:88、SEQ IDNO:97、SEQ ID NO:98、SEQ ID NO:99、SEQ ID NO:100、SEQ ID NO:105、SEQ ID NO:106、SEQID NO:115、SEQ ID NO:116、SEQ ID NO:117、SEQ ID NO:118、SEQ ID NO:131、SEQ ID NO:132、SEQ ID NO:139、SEQ ID NO:140、SEQ ID NO:145、SEQ ID NO:146、SEQ ID NO:147、SEQID NO:148、SEQ ID NO:165、SEQ ID NO:166、SEQ ID NO:171、SEQ ID NO:172、SEQ ID NO:173、SEQ ID NO:174、SEQ ID NO:177、SEQ ID NO:178、SEQ ID NO:179、SEQ ID NO:180、SEQID NO:181、SEQ ID NO:182、SEQ ID NO:183、SEQ ID NO:184、SEQ ID NO:187、SEQ ID NO:188、SEQ ID NO:195、SEQ ID NO:196、SEQ ID NO:197、SEQ ID NO:198、SEQ ID NO:205、SEQID NO:206、SEQ ID NO:213、SEQ ID NO:214、SEQ ID NO:215、SEQ ID NO:216、SEQ ID NO:227、SEQ ID NO:228、SEQ ID NO:237、SEQ ID NO:238、SEQ ID NO:239、SEQ ID NO:240、SEQID NO:243、SEQ ID NO:244、SEQ ID NO:253、SEQ ID NO:254、SEQ ID NO:255、SEQ ID NO:256、SEQ ID NO:257、SEQ ID NO:258、SEQ ID NO:263、SEQ ID NO:264、SEQ ID NO:265、SEQID NO:266、SEQ ID NO:267、SEQ ID NO:268、SEQ ID NO:271、SEQ ID NO:272、SEQ ID NO:273、SEQ ID NO:274、SEQ ID NO:281、SEQ ID NO:282、SEQ ID NO:283、SEQ ID NO:284、SEQID NO:287、SEQ ID NO:288、SEQ ID NO:295、SEQ ID NO:296、SEQ ID NO:299、SEQ ID NO:300、SEQ ID NO:301、SEQ ID NO:302、SEQ ID NO:303、SEQ ID NO:304、SEQ ID NO:313、SEQID NO:314、SEQ ID NO:315、SEQ ID NO:316、SEQ ID NO:321、SEQ ID NO:322、SEQ ID NO:339、SEQ ID NO:340、SEQ ID NO:347、SEQ ID NO:348、SEQ ID NO:355、SEQ ID NO:356、SEQID NO:375、SEQ ID NO:376、SEQ ID NO:379、SEQ ID NO:380、SEQ ID NO:381、SEQ ID NO:382、SEQ ID NO:383、SEQ ID NO:384、SEQ ID NO:393、SEQ ID NO:394、SEQ ID NO:395、SEQID NO:396、SEQ ID NO:397、SEQ ID NO:398、SEQ ID NO:399、SEQ ID NO:400、SEQ ID NO:407、SEQ ID NO:408、SEQ ID NO:411、SEQ ID NO:412、SEQ ID NO:423、SEQ ID NO:424、SEQID NO:429、SEQ ID NO:430、SEQ ID NO:435、SEQ ID NO:436、SEQ ID NO:437、SEQ ID NO:438、SEQ ID NO:441、SEQ ID NO:442、SEQ ID NO:451、SEQ ID NO:452、SEQ ID NO:463、SEQID NO:464、SEQ ID NO:465、SEQ ID NO:466、SEQ ID NO:473、SEQ ID NO:474、SEQ ID NO:475、SEQ ID NO:476、SEQ ID NO:477、SEQ ID NO:478、SEQ ID NO:479、SEQ ID NO:480、SEQID NO:491、SEQ ID NO:492、SEQ ID NO:493、SEQ ID NO:494、SEQ ID NO:495、SEQ ID NO:496、SEQ ID NO:497、SEQ ID NO:498、SEQ ID NO:501、SEQ ID NO:502、SEQ ID NO:507、SEQID NO:508、SEQ ID NO:511、SEQ ID NO:512、SEQ ID NO:513、SEQ ID NO:514、SEQ ID NO:515、SEQ ID NO:516、SEQ ID NO:517、SEQ ID NO:518、SEQ ID NO:519、SEQ ID NO:520、SEQID NO:523、SEQ ID NO:524、SEQ ID NO:529、SEQ ID NO:530、SEQ ID NO:531、SEQ ID NO:532、SEQ ID NO:533、SEQ ID NO:534、SEQ ID NO:535、SEQ ID NO:536、SEQ ID NO:539、SEQID NO:540、SEQ ID NO:541、SEQ ID NO:542、SEQ ID NO:549、SEQ ID NO:550、SEQ ID NO:553、SEQ ID NO:554、SEQ ID NO:557、SEQ ID NO:558、SEQ ID NO:559、SEQ ID NO:560、SEQID NO:563、SEQ ID NO:564、SEQ ID NO:567、SEQ ID NO:568、SEQ ID NO:571、SEQ ID NO:572、SEQ ID NO:573、SEQ ID NO:574、SEQ ID NO:579、SEQ ID NO:580、SEQ ID NO:583、SEQID NO:584、SEQ ID NO:605、SEQ ID NO:606、SEQ ID NO:607、SEQ ID NO:608、SEQ ID NO:611、SEQ ID NO:612、SEQ ID NO:615、SEQ ID NO:616、SEQ ID NO:621、SEQ ID NO:622、SEQID NO:623、SEQ ID NO:624、SEQ ID NO:627、SEQ ID NO:628、SEQ ID NO:629、SEQ ID NO:630、SEQ ID NO:635、SEQ ID NO:636、SEQ ID NO:643、SEQ ID NO:644、SEQ ID NO:649、SEQID NO:650、SEQ ID NO:661、SEQ ID NO:662、SEQ ID NO:665、SEQ ID NO:666、SEQ ID NO:673、SEQ ID NO:674、SEQ ID NO:685、SEQ ID NO:686、SEQ ID NO:687、SEQ ID NO:688、SEQID NO:691、SEQ ID NO:692、SEQ ID NO:693、SEQ ID NO:694、SEQ ID NO:695、SEQ ID NO:696、SEQ ID NO:703、SEQ ID NO:704、SEQ ID NO:705、SEQ ID NO:706、SEQ ID NO:713、SEQID NO:714、SEQ ID NO:717、SEQ ID NO:718、SEQ ID NO:723、SEQ ID NO:724、SEQ ID NO:725、SEQ ID NO:726、SEQ ID NO:727、SEQ ID NO:728、SEQ ID NO:729、SEQ ID NO:730、SEQID NO:731、SEQ ID NO:732、SEQ ID NO:739、SEQ ID NO:740、SEQ ID NO:741、SEQ ID NO:742、SEQ ID NO:743、SEQ ID NO:744、SEQ ID NO:747、SEQ ID NO:748、SEQ ID NO:749、SEQID NO:750、SEQ ID NO:751、SEQ ID NO:752、SEQ ID NO:753、SEQ ID NO:754、SEQ ID NO:755、SEQ ID NO:756、SEQ ID NO:767、SEQ ID NO:768、SEQ ID NO:769、SEQ ID NO:770、SEQID NO:775、SEQ ID NO:776、SEQ ID NO:779、SEQ ID NO:780、SEQ ID NO:807、SEQ ID NO:808、SEQ ID NO:813、SEQ ID NO:814、SEQ ID NO:823、SEQ ID NO:824、SEQ ID NO:831、SEQID NO:832、SEQ ID NO:833、SEQ ID NO:834、SEQ ID NO:835、SEQ ID NO:836、SEQ ID NO:843、SEQ ID NO:844、SEQ ID NO:845、SEQ ID NO:846、SEQ ID NO:851、SEQ ID NO:852、SEQID NO:857、SEQ ID NO:858、SEQ ID NO:861、SEQ ID NO:862、SEQ ID NO:871、SEQ ID NO:872、SEQ ID NO:875、SEQ ID NO:876、SEQ ID NO:885、SEQ ID NO:886、SEQ ID NO:893、SEQID NO:894、SEQ ID NO:909、SEQ ID NO:910、SEQ ID NO:911、SEQ ID NO:912、SEQ ID NO:913、SEQ ID NO:914、SEQ ID NO:915、SEQ ID NO:916、SEQ ID NO:923、SEQ ID NO:924、SEQID NO:927、SEQ ID NO:928、SEQ ID NO:929、SEQ ID NO:930、SEQ ID NO:933、SEQ ID NO:934、SEQ ID NO:937、SEQ ID NO:938、SEQ ID NO:939、SEQ ID NO:940、SEQ ID NO:947、SEQID NO:948、SEQ ID NO:951、SEQ ID NO:952、SEQ ID NO:953、SEQ ID NO:954、SEQ ID NO:959、SEQ ID NO:960、SEQ ID NO:961、SEQ ID NO:962、SEQ ID NO:965、SEQ ID NO:966、SEQID NO:967、SEQ ID NO:968、SEQ ID NO:971、SEQ ID NO:972、SEQ ID NO:977、SEQ ID NO:978、SEQ ID NO:989、SEQ ID NO:990、SEQ ID NO:999和SEQ ID NO:1000所示。
本发明还保护上述任一所述引物组合甲或上述任一所述引物组合乙在香菇品种鉴定中的应用。
本发明还保护上述任一所述引物组合甲或上述任一所述引物组合乙在鉴别香菇品种真伪性中的应用。
本发明还保护上述任一所述引物组合甲或上述任一所述引物组合乙在鉴定待测香菇菌株是否为同一品种或同一谱系中的应用。
本发明还保护一种鉴定若干待测香菇菌株是否为同一品种或同一谱系的方法,依次可包括如下步骤:
(1)分别以若干待测香菇菌株的基因组DNA为模板,采用上述任一所述引物组合甲或上述任一所述引物组合乙进行多重PCR扩增,得到PCR扩增产物;
(2)分别将PCR扩增产物进行测序;
(3)分别将PCR扩增产物测序结果与香菇MNP标记文库中进行比对,计算待测香菇菌株和香菇MNP标记文库中菌株间的两两遗传相似度GS,之后进行如下判断:如果遗传相似度GS小于94%,则两个待测香菇菌株为不同品种且不同谱系;如果遗传相似度GS为94%以上且小于99.5%,则两个待测香菇菌株为同一谱系内的不同品种;如果遗传相似度GS为99.5%以上,则两个待测香菇菌株为同一谱系内的同一品种。
上述方法中,所述香菇MNP标记文库的制备方法可如下:分别提取具有代表性的香菇菌株的基因组DNA,采用上述任一所述引物组合甲或上述任一所述引物组合乙进行多重PCR扩增,得到PCR扩增产物;之后测序,所有测序结果组成香菇MNP标记文库。
本发明采用基于SNP标记法发展而来的多核苷酸多态性标记(MNP)法,开发了MNP标记用于香菇的精准鉴定,并制定相关流程实现香菇品种鉴定。发明人首先基于187份香菇全基因组数据,以125bp碱基为窗口筛选SNP位点数大于2且小于10的区段为候选区段,随后对候选区段进一步筛选,最终设计并合成得到501个引物对作为MNP标记。利用所开发的501个引物对对78份香菇商业品种进行多重扩增、MNP-Seq测序,构建MNP标记位点数据库;数据分析表明每个样品平均检出的MNP标记达472.96个,平均检出率高达94.4%。为了检测所开发的MNP标记的重现性和准确性,分别对15个品种的相同DNA建立两次文库,结果表明可重复的基因型对数为7601,重现性为99.92%,准确性为99.96%。可见,501个引物对组成的MNP标记的重现性、准确性和检出率都较高。
本发明还通过计算具有代表性的78份香菇样品两两样品间的MNP位点差异比例和遗传相似度(GS),建立了评价两个品种关系的判断标准:如果两个品种间遗传相似度GS<94%,则两品种为“不同品种且不同谱系”;如果两个品种间94%≤遗传相似度GS<99.5%,则两品种为“相同谱系内的不同品种”;如果两个品种间遗传相似度GS≥99.5%,则两品种为“相同品种”。与现有技术相比,本发明具有以下优点:1)引物来源于市面常见的香菇菌株材料,鉴定具有代表性;2)本发明提供的501个引物对的靶序列覆盖整个香菇基因组,相对于传统的SSR标记检测位点更全面;3)此外虽然引物较多,但采用Multi PCR技术和高通量测序只需一次扩增反应即可完成所有MNP位点的检测,检测成本较低,操作简便,采用MultiPCR技术和高通量测序只需一次扩增反应即可完成所有MNP位点的检测。本发明具有重要的应用价值。
附图说明
图1为部分香菇样品MNP标记检出位点数的分布结果。
图2为基于187个核心MNP标记序列的香菇系统发育树。
保藏说明
微生物名称:Lentinula edodes香菇
株号:5.0501
保藏机构:中国微生物菌种保藏管理委员会普通微生物中心
保藏机构简称:CGMCC
地址:北京市朝阳区北辰西路1号院
保藏日期:2022年3月31日
保藏中心登记编号:CGMCC 5.2232
微生物名称:Lentinula edodes香菇
株号:5.0502
保藏机构:中国微生物菌种保藏管理委员会普通微生物中心
保藏机构简称:CGMCC
地址:北京市朝阳区北辰西路1号院
保藏日期:2022年3月31日
保藏中心登记编号:CGMCC 5.2233
微生物名称:Lentinula edodes香菇
株号:5.0503
保藏机构:中国微生物菌种保藏管理委员会普通微生物中心
保藏机构简称:CGMCC
地址:北京市朝阳区北辰西路1号院
保藏日期:2022年3月31日
保藏中心登记编号:CGMCC 5.2234
微生物名称:Lentinula edodes香菇
株号:5.0504
保藏机构:中国微生物菌种保藏管理委员会普通微生物中心
保藏机构简称:CGMCC
地址:北京市朝阳区北辰西路1号院
保藏日期:2022年3月31日
保藏中心登记编号:CGMCC 5.2235
微生物名称:Lentinula edodes香菇
株号:5.0505
保藏机构:中国微生物菌种保藏管理委员会普通微生物中心
保藏机构简称:CGMCC
地址:北京市朝阳区北辰西路1号院
保藏日期:2022年3月31日
保藏中心登记编号:CGMCC 5.2236
微生物名称:Lentinula edodes香菇
株号:5.0506
保藏机构:中国微生物菌种保藏管理委员会普通微生物中心
保藏机构简称:CGMCC
地址:北京市朝阳区北辰西路1号院
保藏日期:2022年3月31日
保藏中心登记编号:CGMCC 5.2237
微生物名称:Lentinula edodes香菇
株号:5.0507
保藏机构:中国微生物菌种保藏管理委员会普通微生物中心
保藏机构简称:CGMCC
地址:北京市朝阳区北辰西路1号院
保藏日期:2022年3月31日
保藏中心登记编号:CGMCC 5.2238
微生物名称:Lentinula edodes香菇
株号:5.0508
保藏机构:中国微生物菌种保藏管理委员会普通微生物中心
保藏机构简称:CGMCC
地址:北京市朝阳区北辰西路1号院
保藏日期:2022年3月31日
保藏中心登记编号:CGMCC 5.2239
微生物名称:Lentinula edodes香菇
株号:5.0509
保藏机构:中国微生物菌种保藏管理委员会普通微生物中心
保藏机构简称:CGMCC
地址:北京市朝阳区北辰西路1号院
保藏日期:2022年3月31日
保藏中心登记编号:CGMCC 5.2240
微生物名称:Lentinula edodes香菇
株号:5.0510
保藏机构:中国微生物菌种保藏管理委员会普通微生物中心
保藏机构简称:CGMCC
地址:北京市朝阳区北辰西路1号院
保藏日期:2022年3月31日
保藏中心登记编号:CGMCC 5.2241
微生物名称:Lentinula edodes香菇
株号:5.0511
保藏机构:中国微生物菌种保藏管理委员会普通微生物中心
保藏机构简称:CGMCC
地址:北京市朝阳区北辰西路1号院
保藏日期:2022年3月31日
保藏中心登记编号:CGMCC 5.2242
微生物名称:Lentinula edodes香菇
株号:5.0512
保藏机构:中国微生物菌种保藏管理委员会普通微生物中心
保藏机构简称:CGMCC
地址:北京市朝阳区北辰西路1号院
保藏日期:2022年3月31日
保藏中心登记编号:CGMCC 5.2243
微生物名称:Lentinula edodes香菇
株号:5.0513
保藏机构:中国微生物菌种保藏管理委员会普通微生物中心
保藏机构简称:CGMCC
地址:北京市朝阳区北辰西路1号院
保藏日期:2022年3月31日
保藏中心登记编号:CGMCC 5.2244
微生物名称:Lentinula edodes香菇
株号:5.0514
保藏机构:中国微生物菌种保藏管理委员会普通微生物中心
保藏机构简称:CGMCC
地址:北京市朝阳区北辰西路1号院
保藏日期:2022年3月31日
保藏中心登记编号:CGMCC 5.2245
微生物名称:Lentinula edodes香菇
株号:5.0515
保藏机构:中国微生物菌种保藏管理委员会普通微生物中心
保藏机构简称:CGMCC
地址:北京市朝阳区北辰西路1号院
保藏日期:2022年3月31日
保藏中心登记编号:CGMCC 5.2246
微生物名称:Lentinula edodes香菇
株号:5.0516
保藏机构:中国微生物菌种保藏管理委员会普通微生物中心
保藏机构简称:CGMCC
地址:北京市朝阳区北辰西路1号院
保藏日期:2022年3月31日
保藏中心登记编号:CGMCC 5.2247
微生物名称:Lentinula edodes香菇
株号:5.0517
保藏机构:中国微生物菌种保藏管理委员会普通微生物中心
保藏机构简称:CGMCC
地址:北京市朝阳区北辰西路1号院
保藏日期:2022年3月31日
保藏中心登记编号:CGMCC 5.2248
微生物名称:Lentinula edodes香菇
株号:5.0518
保藏机构:中国微生物菌种保藏管理委员会普通微生物中心
保藏机构简称:CGMCC
地址:北京市朝阳区北辰西路1号院
保藏日期:2022年3月31日
保藏中心登记编号:CGMCC 5.2249
微生物名称:Lentinula edodes香菇
株号:5.0519
保藏机构:中国微生物菌种保藏管理委员会普通微生物中心
保藏机构简称:CGMCC
地址:北京市朝阳区北辰西路1号院
保藏日期:2022年3月31日
保藏中心登记编号:CGMCC 5.2250
微生物名称:Lentinula edodes香菇
株号:5.0521
保藏机构:中国微生物菌种保藏管理委员会普通微生物中心
保藏机构简称:CGMCC
地址:北京市朝阳区北辰西路1号院
保藏日期:2022年3月31日
保藏中心登记编号:CGMCC 5.2252
微生物名称:Lentinula edodes香菇
株号:5.0522
保藏机构:中国微生物菌种保藏管理委员会普通微生物中心
保藏机构简称:CGMCC
地址:北京市朝阳区北辰西路1号院
保藏日期:2022年3月31日
保藏中心登记编号:CGMCC 5.2253
具体实施方式
下面结合具体实施方式对本发明进行进一步的详细描述,给出的实施例仅为了阐明本发明,而不是为了限制本发明的范围。以下提供的实施例可作为本技术领域普通技术人员进行进一步改进的指南,并不以任何方式构成对本发明的限制。
下述实施例中的实验方法,如无特殊说明,均为常规方法,按照本领域内的文献所描述的技术或条件或者按照产品说明书进行。下述实施例中所用的材料、试剂等,如无特殊说明,均可从商业途径得到。
下述实施例涉及的香菇品种的基本信息见表1中第1至4列。
表1.香菇品种的基本信息
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注:黑体加粗标注的菌株为重测序菌株。
Lentinula edodes香菇5.0501、Lentinula edodes香菇5.0502、Lentinulaedodes香菇5.0503、Lentinula edodes香菇5.0504、Lentinula edodes香菇5.0505、Lentinula edodes香菇5.0506、Lentinula edodes香菇5.0507、Lentinula edodes香菇5.0508、Lentinula edodes香菇5.0509、Lentinula edodes香菇5.0510、Lentinula edodes香菇5.0511、Lentinula edodes香菇5.0512、Lentinula edodes香菇5.0513、Lentinulaedodes香菇5.0514、Lentinula edodes香菇5.0515、Lentinula edodes香菇5.0516、Lentinula edodes香菇5.0517、Lentinula edodes香菇5.0518、Lentinula edodes香菇5.0519、Lentinula edodes香菇5.0521和Lentinula edodes香菇5.0522在本申请中的名称依次为:白花2号、庆科20变种、香菇品种L109、大春-1、春栽208、矮花3号、香9608-WY、香农9608、森源16号、香菇品种807(中香36)、台引、辽抚10号、高香1号、菌兴68、香菇品种922、香菇品种218、香农49、香菇野生菌株GX20170368、香菇野生菌株ZRL20180417、香24、香菇野生菌株GX20170702。
实施例1、用于扩增香菇广泛MNP标记位点的引物对筛选
1、本申请的发明人通过重测序,获得25株香菇菌株全基因组重测序数据。
2、25株香菇菌株分别为广香51、香菇品种L135、香菇品种241-4、武香1号、香菇品种L808、香菇品种Cr02、沪农1号、森源8404、香菇品种L9015、香九、香菇品种7401、香菇品种L12、香菇品种L26、香菇品种L087、苏香、久香秋7、大春-1、矮花3号、香菇野生菌株GX20170368、香菇野生菌株ZRL20180417、香菇野生菌株ZRL20180817、香菇野生菌株5.0439、香24、香菇野生菌株5.0491和香菇野生菌株GX20170702。
3、对162份NCBI上的香菇菌株全基因组重测序数据(涉及162株香菇菌株)和25株香菇菌株全基因组重测序数据(自行获得,)进行生物信息学分析。具体步骤如下:首先使用Bowtie2将重测序数据和香菇参考基因组(基因组版本:GCA_001562095.1_B17_genomic.fna.gz)进行比对;之后使用SAMtools-mpileup分析获得所有个体的SNP,从所有SNP存在区段中,使用125个碱基对(bp)的滑动窗口,以5bp的增量扫描基因组,筛选没有SSR、没有连续SNP的窗口作为目标区段;通过分析共筛选得到501个香菇广泛MNP标记位点,并根据香菇广泛MNP标记位点的位置设计并合成501个引物对。引物对设计的原则包括:扩增区保守、单拷贝、多态性高、在不同样品中的缺失率低等;保证每个引物的退火温度一致、不形成二聚体、在基因组上扩增特异。
用于扩增501个香菇广泛MNP标记位点的引物对的引物序列等信息见表2。
表2
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注:加粗字体为用于扩增核心MNP标记位点的引物对。
实施例2、香菇MNP标记文库的建立
本申请的发明人从实施例1中187株香菇菌株中选取具有代表性的78株香菇菌株(具体为表1中序号1至序号78的78株香菇菌株),包括25个国家品种委员会认定的香菇商业栽培品种、7个通过地方审定(鉴定、备案)品种、以及具有代表性状、生物学特性和来自不同地域的栽培菌株。品种名称、品种认定情况、品种来源及其谱系情况详见表1。
分别提取78株香菇菌株的基因组DNA,采用实施例1中合成的用于扩增501个香菇广泛MNP标记位点的引物对进行多重PCR扩增、测序以及数据分析。结果表明,共完成了37830个标记的检测,每个菌株的平均测序覆盖率为3464.904倍,构建了香菇MNP标记文库(即78株菌株的MNP-Seq数据),文库基本信息见表3。
表3.香菇MNP标记文库基本信息
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实施例3、香菇MNP标记文库中香菇广泛MNP标记位点检出率、重现性和准确性检测
1、对实施例2构建的香菇MNP标记文库中的MNP-Seq数据进行分析。
结果表明,每个菌株分别可检出250-497个MNP标记位点,平均每个菌株可以检出472.96个MNP标记,检出率约为94.4%,MNP标记检出位点数分布结果见图1。由此可见,实施例2获得的MNP标记位点具有较高检出率。
2、从实施例2中78株香菇菌株中随机选择15株,进行重现性和准确性实验。具体步骤为:分别提取15株香菇菌株的基因组DNA,将香菇菌株的基因组DNA等量均分;之后将同一香菇菌株的两份基因组DNA采用实施例1中合成的用于扩增501个香菇广泛MNP标记位点的引物对进行多重PCR扩增、先后测序(即不同时间测序)以及数据分析,获得第一批次香菇MNP标记文库和第二批次香菇MNP标记文库。比较第一批次香菇MNP标记文库和第二批次香菇MNP标记文库中差异位点数目,根据重现性计算公式分别计算出每个菌株的重现性(n:可重复的基因型对数,N:比较的基因型总对数,r:重现性):
合计所得的基因型总对数为7607,可重复的基因型对数为7601,计算可得,重现性为99.92%,每个菌株的重现性见表4。
第一批次香菇MNP标记文库和第二批次香菇MNP标记文库之间可重复的基因型可以被认为是准确的,根据准确性性计算公式,分别计算出每个菌株的准确性:
计算可得,准确性为99.96%,每个菌株的准确性见表4。
表4
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实施例4、香菇核心MNP标记位点的确定
从501个香菇广泛MNP标记位点中,选择对供试的78株香菇菌株扩增率为100%的位点作为香菇的核心MNP标记位点,共得到187个香菇核心MNP标记位点。
用于扩增187个香菇核心MNP标记位点的引物对的引物序列信息见表2中加粗字体。
参考实施例3的方法,采用187个香菇核心MNP标记位点构建香菇MNP标记核心文库,该文库的香菇核心MNP标记位点检出率、重现性和准确性均为100%。
实施例5、香菇两两菌株间遗传相似度的计算方法
本申请的发明人经过大量实验,建立了香菇两两菌株间遗传相似度的方法,具体步骤如下:
1、将待测香菇菌株分别进行总DNA提取。
2、采用187个香菇核心MNP标记位点的混合引物对进行多重PCR扩增。
3、扩增产物送往测序公司进行测序,得187个核心MNP标记位点的序列。
4、将上述菌株的多重PCR测序结果与香菇MNP标记核心文库中所有菌株的位点序列进行比对,并计算待测菌株和文库中所有品种的两两遗传相似度。
具体方法为:除去MNP位点序列中空值“N”后,把待测香菇菌株的MNP序列和文库中所有品种的核心MNP标记位点序列分别进行两两比较。待测香菇菌株在同一MNP位点上的序列完全相同(杂合位点的兼并碱基与正常碱基视为相同)则认为它们在该位点具有相同的基因型,该位点记作相同位点;否则为不同基因型,记作差异位点。分别统计待测香菇菌株和文库中所有品种间共有的非空值(即序列中的“N”)MNP差异位点数(Nij)、相同位点数(nij),根据下述公式计算遗传相似度(GS):
实施例6、利用187个香菇核心MNP标记位点确定谱系和品种
1、获得240株香菇菌株187个香菇核心MNP标记位点序列数据,包括实施例1中162份NCBI上的香菇菌株全基因组重测序数据中提取出的和本申请的发明人对实施例2中78株香菇菌株187个香菇核心MNP标记位点测序获得的数据。
2、从240株香菇菌株187个香菇核心MNP标记位点序列数据构建187个核心MNP标记位点序列的ML系统发育树(见图2)。结合系统发育树和所有商业栽培菌株两两之间的GS(见表5),所有供试的商业栽培菌株可以划分为24个谱系(见图2),具体标准为:如果两个品种间遗传相似度GS<94%,则两品种为“不同品种且不同谱系”;如果两个品种间94%≤遗传相似度GS<99.5%,则两品种为“相同谱系内的不同品种”;如果两个品种间遗传相似度GS≥99.5%,则两品种为“相同品种”。
表5-1. 24个谱系内及谱系间的遗传相似度
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表5-2. 24个谱系内及谱系间的遗传相似度
G9 | G10 | G11 | G12 | G13 | G14 | G15 | G16 | |
G1 | 13.24% | 17.62% | 14.24% | 16.45% | 19.13% | 20.77% | 18.65% | 15.57% |
G2 | 73.21% | 75.14% | 71.83% | 75.67% | 77.27% | 73.53% | 74.09% | 66.31% |
G3 | 70.78% | 70.82% | 72.24% | 73.35% | 75.94% | 74.33% | 74.36% | 63.90% |
G4 | 68.82% | 69.39% | 66.49% | 74.50% | 72.43% | 69.73% | 76.02% | 61.62% |
G5 | 70.67% | 82.01% | 69.39% | 76.44% | 71.40% | 73.02% | 77.38% | 57.37% |
G6 | 64.88% | 65.22% | 62.91% | 69.19% | 67.83% | 69.01% | 68.41% | 57.97% |
G7 | 68.94% | 81.67% | 69.58% | 72.64% | 70.94% | 72.48% | 73.95% | 61.30% |
G8 | 93.96% | 89.84% | 92.22% | 86.10% | 94.27% | 88.47% | 82.64% | 89.67% |
G9 | 96.20% | 89.34% | 90.53% | 80.55% | 86.72% | 82.67% | 77.95% | 83.75% |
G10 | 89.34% | 94.54% | 88.47% | 80.12% | 83.24% | 80.81% | 81.55% | 80.41% |
G11 | 90.53% | 88.47% | 98.01% | 79.58% | 86.52% | 83.96% | 82.21% | 78.51% |
G12 | 80.55% | 80.12% | 79.58% | 98.74% | 90.52% | 84.08% | 92.61% | 78.44% |
G13 | 86.72% | 83.24% | 86.52% | 90.52% | 100.00% | 94.12% | 91.81% | 89.30% |
G14 | 82.67% | 80.81% | 83.96% | 84.08% | 94.12% | 100.00% | 93.45% | 77.81% |
G15 | 77.95% | 81.55% | 82.21% | 92.61% | 91.81% | 93.45% | 98.35% | 80.51% |
G16 | 83.75% | 80.41% | 78.51% | 78.44% | 89.30% | 77.81% | 80.51% | 97.86% |
G17 | 81.81% | 83.39% | 82.21% | 88.32% | 85.34% | 80.13% | 86.91% | 86.09% |
G18 | 85.93% | 74.90% | 84.22% | 79.06% | 90.05% | 79.68% | 80.24% | 82.30% |
G19 | 92.42% | 82.70% | 88.95% | 85.69% | 87.17% | 86.10% | 81.19% | 79.95% |
G20 | 88.35% | 81.89% | 91.24% | 81.57% | 85.56% | 83.42% | 79.83% | 80.21% |
G21 | 86.47% | 76.63% | 84.80% | 77.72% | 88.93% | 79.51% | 78.51% | 81.77% |
G22 | 81.80% | 79.49% | 83.78% | 74.10% | 85.15% | 77.82% | 78.22% | 77.37% |
G23 | 84.50% | 80.67% | 93.65% | 73.02% | 89.16% | 81.83% | 76.87% | 77.69% |
G24 | 70.50% | 83.28% | 83.02% | 71.92% | 73.26% | 74.33% | 74.37% | 66.58% |
表5-3. 24个谱系内及谱系间的遗传相似度
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以上对本发明进行了详述。对于本领域技术人员来说,在不脱离本发明的宗旨和范围,以及无需进行不必要的实验情况下,可在等同参数、浓度和条件下,在较宽范围内实施本发明。虽然本发明给出了特殊的实施例,应该理解为,可以对本发明作进一步的改进。总之,按本发明的原理,本申请欲包括任何变更、用途或对本发明的改进,包括脱离了本申请中已公开范围,而用本领域已知的常规技术进行的改变。按以下附带的权利要求的范围,可以进行一些基本特征的应用。
<110>中国科学院微生物研究所 江汉大学
<120>一种鉴定香菇品种的MNP标记法的开发及应用
<160>1002
<170>PatentIn version 3.5
<210> 1
<211> 25
<212> DNA
<213> Artificial sequence
<400> 1
ATTATCCAAA TGAAAGGAGC GTGTG 25
<210> 2
<211> 25
<212> DNA
<213> Artificial sequence
<400> 2
TGCTACAGTA CACGTCTGAA TTGTA 25
<210> 3
<211> 25
<212> DNA
<213> Artificial sequence
<400> 3
TCCCTCTTGT TGCTTATCTT CAAGA 25
<210> 4
<211> 25
<212> DNA
<213> Artificial sequence
<400> 4
TCGAATGAGT TTTGGAGAGT ACAGT 25
<210> 5
<211> 25
<212> DNA
<213> Artificial sequence
<400> 5
ATGCACACTC ATACTCTCAC ATCTT 25
<210> 6
<211> 25
<212> DNA
<213> Artificial sequence
<400> 6
ATAACAATGG ATTTACGGAA CTGCC 25
<210> 7
<211> 25
<212> DNA
<213> Artificial sequence
<400> 7
CTGAGACAAC GCTCAGTATA TACGA 25
<210> 8
<211> 25
<212> DNA
<213> Artificial sequence
<400> 8
ATAGATGTAT GATGCAAGAT GGCTG 25
<210> 9
<211> 25
<212> DNA
<213> Artificial sequence
<400> 9
GTCTAAAGCG TTGTTTTGAA AGCAG 25
<210> 10
<211> 25
<212> DNA
<213> Artificial sequence
<400> 10
CGTCGGAAAA GACTAGCTGA AATTA 25
<210> 11
<211> 25
<212> DNA
<213> Artificial sequence
<400> 11
ACGGTTGCAA AAATCAACGA ATATG 25
<210> 12
<211> 25
<212> DNA
<213> Artificial sequence
<400> 12
GGAGTCGCTG TACTACTGTA TGATT 25
<210> 13
<211> 25
<212> DNA
<213> Artificial sequence
<400> 13
GATATGTTCG CTACATCGGA ATGTC 25
<210> 14
<211> 25
<212> DNA
<213> Artificial sequence
<400> 14
GAAACATTTC TCGTTCCTCT TCTCG 25
<210> 15
<211> 25
<212> DNA
<213> Artificial sequence
<400> 15
GGATAATGGA TGGATTTTGG AGGTG 25
<210> 16
<211> 23
<212> DNA
<213> Artificial sequence
<400> 16
CAGAACAGAT GCAGAGCTTG TTC 23
<210> 17
<211> 25
<212> DNA
<213> Artificial sequence
<400> 17
TGGTAGTTAA TTTAGGAACG CCACT 25
<210> 18
<211> 25
<212> DNA
<213> Artificial sequence
<400> 18
TTTAGAAGCT GCATGTTGAT TGGTT 25
<210> 19
<211> 25
<212> DNA
<213> Artificial sequence
<400> 19
GATCCGGCAT TGCAAATATC AGTAT 25
<210> 20
<211> 25
<212> DNA
<213> Artificial sequence
<400> 20
TACAAACTAC ACATCACCTT GCAAT 25
<210> 21
<211> 25
<212> DNA
<213> Artificial sequence
<400> 21
CCAGGTATGC AATCAGGGAA TTAAC 25
<210> 22
<211> 25
<212> DNA
<213> Artificial sequence
<400> 22
CCACTGCATC TTTGTGAAGT CATTA 25
<210> 23
<211> 25
<212> DNA
<213> Artificial sequence
<400> 23
GGAATTCACG GCAAGTTGTA GAAAT 25
<210> 24
<211> 25
<212> DNA
<213> Artificial sequence
<400> 24
TTCTTTCTGT TCAGATGCAC AACTC 25
<210> 25
<211> 25
<212> DNA
<213> Artificial sequence
<400> 25
TGTGAAAAGA TTCAAAACCT TGGGT 25
<210> 26
<211> 25
<212> DNA
<213> Artificial sequence
<400> 26
AGAGCTAAAC AACAGAATTT GAGGC 25
<210> 27
<211> 25
<212> DNA
<213> Artificial sequence
<400> 27
GGACAGTGTG ATATCTTGTG GTACT 25
<210> 28
<211> 25
<212> DNA
<213> Artificial sequence
<400> 28
ACAACAGATG GTGAAGCAAA ATGAT 25
<210> 29
<211> 25
<212> DNA
<213> Artificial sequence
<400> 29
AGGTCATACC AAAGGAAGGT CATAC 25
<210> 30
<211> 25
<212> DNA
<213> Artificial sequence
<400> 30
TCGTTTATCG TTTATCGTTG TGTCC 25
<210> 31
<211> 25
<212> DNA
<213> Artificial sequence
<400> 31
ATTTTTACAT GGGCAAGCCA AAAAG 25
<210> 32
<211> 25
<212> DNA
<213> Artificial sequence
<400> 32
ACCATTGAGA ACATACTGTA GAGCT 25
<210> 33
<211> 25
<212> DNA
<213> Artificial sequence
<400> 33
CTCACCATAT TGCATTGTGC CTAAG 25
<210> 34
<211> 25
<212> DNA
<213> Artificial sequence
<400> 34
GACGGATCCT CGAAGATTCT AAAGA 25
<210> 35
<211> 26
<212> DNA
<213> Artificial sequence
<400> 35
TCAGCCTAGT GTATTATCAT CATGCT 26
<210> 36
<211> 25
<212> DNA
<213> Artificial sequence
<400> 36
GCCCCTAGTC TATAAACCCC AAAAA 25
<210> 37
<211> 25
<212> DNA
<213> Artificial sequence
<400> 37
ATCTTTGCCT TCAAGTTTGA GTGTT 25
<210> 38
<211> 25
<212> DNA
<213> Artificial sequence
<400> 38
TAGTCTACTA CACCCGATTC AACAG 25
<210> 39
<211> 23
<212> DNA
<213> Artificial sequence
<400> 39
TTTCATTCGA ACCCGTCAAA ACC 23
<210> 40
<211> 25
<212> DNA
<213> Artificial sequence
<400> 40
AGGAATTACA ATCAATGATC TGGGT 25
<210> 41
<211> 25
<212> DNA
<213> Artificial sequence
<400> 41
TGTAATGAAT CTGTTCACGA ACTGA 25
<210> 42
<211> 22
<212> DNA
<213> Artificial sequence
<400> 42
GTCGCAGCAA CGATTACACT TG 22
<210> 43
<211> 25
<212> DNA
<213> Artificial sequence
<400> 43
AAGACTTGAG ATACTCAACC CTTGG 25
<210> 44
<211> 25
<212> DNA
<213> Artificial sequence
<400> 44
ATTTCACCAG AATGCGGTCT CTATT 25
<210> 45
<211> 25
<212> DNA
<213> Artificial sequence
<400> 45
AATGCTTCCT AGTAGGTTAC TGGAC 25
<210> 46
<211> 25
<212> DNA
<213> Artificial sequence
<400> 46
CTGTGATGTT GACCAAGGAA AGAAG 25
<210> 47
<211> 25
<212> DNA
<213> Artificial sequence
<400> 47
AGAAGGATTG AGAAGGATTG TCAGA 25
<210> 48
<211> 23
<212> DNA
<213> Artificial sequence
<400> 48
ATCATTTTGA TTTTGGGCGC CTG 23
<210> 49
<211> 25
<212> DNA
<213> Artificial sequence
<400> 49
CGGAGCAGGT AGGATAAGGT AAAAT 25
<210> 50
<211> 25
<212> DNA
<213> Artificial sequence
<400> 50
TACTATCTAT CCTCCTATCC CTGCC 25
<210> 51
<211> 25
<212> DNA
<213> Artificial sequence
<400> 51
CTACTGTTTT TGACCTCCTC TCTGA 25
<210> 52
<211> 25
<212> DNA
<213> Artificial sequence
<400> 52
CCTTGTGATA ACAAGCCTTG GAATT 25
<210> 53
<211> 25
<212> DNA
<213> Artificial sequence
<400> 53
GAAAGCGAAC TTGGTAGTAT GGTTC 25
<210> 54
<211> 25
<212> DNA
<213> Artificial sequence
<400> 54
TCAATGAGAC ATGCGTCAGT ATTTG 25
<210> 55
<211> 26
<212> DNA
<213> Artificial sequence
<400> 55
AAGATTGCTC AATATCTCAA GAATGA 26
<210> 56
<211> 25
<212> DNA
<213> Artificial sequence
<400> 56
GCAGTGGTAA TTGTTACACA AGAGT 25
<210> 57
<211> 26
<212> DNA
<213> Artificial sequence
<400> 57
AAGTAGATTG ATTGGATGGT TCTACA 26
<210> 58
<211> 25
<212> DNA
<213> Artificial sequence
<400> 58
AGTCTGGCAT ATGTTCAGCT ACTTC 25
<210> 59
<211> 26
<212> DNA
<213> Artificial sequence
<400> 59
ACGTTCCCCT TGAAATTATC ATTGAG 26
<210> 60
<211> 25
<212> DNA
<213> Artificial sequence
<400> 60
ATCGAAGAAG AGACGAGCAT ATTGA 25
<210> 61
<211> 25
<212> DNA
<213> Artificial sequence
<400> 61
CGGAATTGAA ACAGAGATAA CCACC 25
<210> 62
<211> 23
<212> DNA
<213> Artificial sequence
<400> 62
TTGTGTACTG GGAGTGGGAT GTC 23
<210> 63
<211> 25
<212> DNA
<213> Artificial sequence
<400> 63
TGAGAGTAGT CTAATGCCAA CGTAG 25
<210> 64
<211> 25
<212> DNA
<213> Artificial sequence
<400> 64
TCGTGACATA AGGAACTGTC TTCAT 25
<210> 65
<211> 25
<212> DNA
<213> Artificial sequence
<400> 65
TGATAGTCAT TGAGGTTGAG GTCAA 25
<210> 66
<211> 25
<212> DNA
<213> Artificial sequence
<400> 66
GCATCCCAAA AACTCATCAT GATCT 25
<210> 67
<211> 25
<212> DNA
<213> Artificial sequence
<400> 67
CAGATTTTGT GTAGGATTGC CATGT 25
<210> 68
<211> 25
<212> DNA
<213> Artificial sequence
<400> 68
TCAATCTTGG GTCCTAGTGA TTCTG 25
<210> 69
<211> 25
<212> DNA
<213> Artificial sequence
<400> 69
GGAAACCGAC TCCTATGTGA TGATA 25
<210> 70
<211> 25
<212> DNA
<213> Artificial sequence
<400> 70
CATTTCTTTT TGATGACGAC GTAGG 25
<210> 71
<211> 24
<212> DNA
<213> Artificial sequence
<400> 71
AAAAAGCCAA TGATGAATGG CAAT 24
<210> 72
<211> 25
<212> DNA
<213> Artificial sequence
<400> 72
TTGACAATCC GTGTAGTTGG GAATA 25
<210> 73
<211> 25
<212> DNA
<213> Artificial sequence
<400> 73
GAAGGAGCAA GGGAAGGTAA TTAGG 25
<210> 74
<211> 25
<212> DNA
<213> Artificial sequence
<400> 74
TGATAATATG TCGGCTTCAT CCAGC 25
<210> 75
<211> 25
<212> DNA
<213> Artificial sequence
<400> 75
CTTCGTGACA TAGACCAGGT AGTTG 25
<210> 76
<211> 25
<212> DNA
<213> Artificial sequence
<400> 76
CTCATCATGA TCTTGTTCCG TTTCC 25
<210> 77
<211> 19
<212> DNA
<213> Artificial sequence
<400> 77
AAACCGCCTT CCCCATACG 19
<210> 78
<211> 25
<212> DNA
<213> Artificial sequence
<400> 78
AAAGTGGGTG CTAATATTTA CCCCA 25
<210> 79
<211> 25
<212> DNA
<213> Artificial sequence
<400> 79
GCACAACAAT CTGTTTCAGC TTCTA 25
<210> 80
<211> 25
<212> DNA
<213> Artificial sequence
<400> 80
ATATACCTTC CGGCTTTCCT TACAA 25
<210> 81
<211> 25
<212> DNA
<213> Artificial sequence
<400> 81
TTATTGAATT TCTGCTCATG TCGGG 25
<210> 82
<211> 25
<212> DNA
<213> Artificial sequence
<400> 82
GTACATACCC TCTTACCATC ATCCC 25
<210> 83
<211> 25
<212> DNA
<213> Artificial sequence
<400> 83
AAAGATCGCA TTCGGTATCC CTAAT 25
<210> 84
<211> 25
<212> DNA
<213> Artificial sequence
<400> 84
AAAAGCGGAA TACAGTACCC AAAAA 25
<210> 85
<211> 25
<212> DNA
<213> Artificial sequence
<400> 85
CCATAGTCAT CCAACTGTGT CCTAT 25
<210> 86
<211> 25
<212> DNA
<213> Artificial sequence
<400> 86
AACACGTACC GAACTATGAT TTGTG 25
<210> 87
<211> 25
<212> DNA
<213> Artificial sequence
<400> 87
CTTTAGCAAA CTTGAGTCCA CATGT 25
<210> 88
<211> 25
<212> DNA
<213> Artificial sequence
<400> 88
GGATGCCATA ATTAAGAACA GGGTG 25
<210> 89
<211> 25
<212> DNA
<213> Artificial sequence
<400> 89
GACAAAGTTC ATCAGCATGC AGAAC 25
<210> 90
<211> 25
<212> DNA
<213> Artificial sequence
<400> 90
AGTTAAACAT AGTGCGAAGA TGGAG 25
<210> 91
<211> 25
<212> DNA
<213> Artificial sequence
<400> 91
GCCTTCTGGA TGATTGTATT TGCAT 25
<210> 92
<211> 25
<212> DNA
<213> Artificial sequence
<400> 92
TGAGATTGAC CCTTGCTTAG CTATC 25
<210> 93
<211> 25
<212> DNA
<213> Artificial sequence
<400> 93
TGCATGAACT TCAGCATACA AATGT 25
<210> 94
<211> 24
<212> DNA
<213> Artificial sequence
<400> 94
CAATCTCTTG CTTTGACCAA CTCA 24
<210> 95
<211> 23
<212> DNA
<213> Artificial sequence
<400> 95
CTCCTTTCTC CTCCCAAAGT CTG 23
<210> 96
<211> 25
<212> DNA
<213> Artificial sequence
<400> 96
AAGAAGGGTG ATTTAGTCCA GTACC 25
<210> 97
<211> 21
<212> DNA
<213> Artificial sequence
<400> 97
CTTCTCCTGT GCCTTCCTCA G 21
<210> 98
<211> 23
<212> DNA
<213> Artificial sequence
<400> 98
TTCGAAGGAA ATTTTTCGCT GGT 23
<210> 99
<211> 22
<212> DNA
<213> Artificial sequence
<400> 99
GCGAAAAGCG ATGTTGAACT CG 22
<210> 100
<211> 23
<212> DNA
<213> Artificial sequence
<400> 100
GTGGGCACAA TTAGCTTCAG AAG 23
<210> 101
<211> 25
<212> DNA
<213> Artificial sequence
<400> 101
TTAAAGATCG CCTACCTCGA TTCAT 25
<210> 102
<211> 23
<212> DNA
<213> Artificial sequence
<400> 102
GGACACGGAC CCATAAAGAT CAA 23
<210> 103
<211> 26
<212> DNA
<213> Artificial sequence
<400> 103
TGTGGATTCA ATCTTACTGT TATCCG 26
<210> 104
<211> 25
<212> DNA
<213> Artificial sequence
<400> 104
CTCAAGAGCA GCTACAGTTC TAGAT 25
<210> 105
<211> 25
<212> DNA
<213> Artificial sequence
<400> 105
AAGTTAGAAC ACTAGTGGCA AATGC 25
<210> 106
<211> 25
<212> DNA
<213> Artificial sequence
<400> 106
CCAGAAACAC TTCCTACCCT TGATA 25
<210> 107
<211> 25
<212> DNA
<213> Artificial sequence
<400> 107
TTGCCTAGGG AACTCTTTTA TACCG 25
<210> 108
<211> 25
<212> DNA
<213> Artificial sequence
<400> 108
GCCGATTAGC TTTCTTCTTA GCAAT 25
<210> 109
<211> 25
<212> DNA
<213> Artificial sequence
<400> 109
TTTACAAGAG TATAATCCGC CGTGA 25
<210> 110
<211> 25
<212> DNA
<213> Artificial sequence
<400> 110
GTAATGTCGA GTAGGATGAT TGGGA 25
<210> 111
<211> 25
<212> DNA
<213> Artificial sequence
<400> 111
CATTGGCAGG TACATAGGTG ATCTA 25
<210> 112
<211> 25
<212> DNA
<213> Artificial sequence
<400> 112
TATAGGCAGT GATGTACAGG AAAGG 25
<210> 113
<211> 25
<212> DNA
<213> Artificial sequence
<400> 113
CTCAAAGTAA GGCTGCAGTT TGTTA 25
<210> 114
<211> 25
<212> DNA
<213> Artificial sequence
<400> 114
AGATGGAAGG ACTGCAATAA GTTCT 25
<210> 115
<211> 28
<212> DNA
<213> Artificial sequence
<400> 115
GCTATGTACT ACTATTACTA ACTCGCCA 28
<210> 116
<211> 25
<212> DNA
<213> Artificial sequence
<400> 116
AAAACTGAAT TTCTGGATAA CCGCG 25
<210> 117
<211> 25
<212> DNA
<213> Artificial sequence
<400> 117
GTGTCGTAGT TTATCAGCGT TCTTG 25
<210> 118
<211> 25
<212> DNA
<213> Artificial sequence
<400> 118
TACGACTTGT ACAGAAATGG CCTTA 25
<210> 119
<211> 25
<212> DNA
<213> Artificial sequence
<400> 119
TATCATGCCC ATCCATTGAC CAATC 25
<210> 120
<211> 25
<212> DNA
<213> Artificial sequence
<400> 120
CCTGATGTTT ATCATTCAGT GAGGC 25
<210> 121
<211> 25
<212> DNA
<213> Artificial sequence
<400> 121
TCATCACGAA ATATGGACTG TACGA 25
<210> 122
<211> 25
<212> DNA
<213> Artificial sequence
<400> 122
CTTTACATAA TAGGCGAACG AGGTG 25
<210> 123
<211> 25
<212> DNA
<213> Artificial sequence
<400> 123
CTTTCTTCAC CATATGTTTG TGGCT 25
<210> 124
<211> 25
<212> DNA
<213> Artificial sequence
<400> 124
TAACTTTCAA TTCCAACCCA TCCTC 25
<210> 125
<211> 25
<212> DNA
<213> Artificial sequence
<400> 125
ATCAAGAAAA GACTTCATGG AGGAT 25
<210> 126
<211> 25
<212> DNA
<213> Artificial sequence
<400> 126
CAAATCTTTG CATTTCTGTT CCTCT 25
<210> 127
<211> 25
<212> DNA
<213> Artificial sequence
<400> 127
GGCAACTTAT ACCAGCCGAT TAATT 25
<210> 128
<211> 25
<212> DNA
<213> Artificial sequence
<400> 128
AGTACTGATA TTGTGTAGTG CGGAA 25
<210> 129
<211> 25
<212> DNA
<213> Artificial sequence
<400> 129
GTCAGGAGTT CCCTTCATAG TTGAT 25
<210> 130
<211> 25
<212> DNA
<213> Artificial sequence
<400> 130
GTCAGTCGAT CCTTCTCTTT TCATT 25
<210> 131
<211> 25
<212> DNA
<213> Artificial sequence
<400> 131
TAATTTGTAA GCCCTGTCAC AACAC 25
<210> 132
<211> 24
<212> DNA
<213> Artificial sequence
<400> 132
ATTCTTGTCT CCATGGCTAT CAGG 24
<210> 133
<211> 25
<212> DNA
<213> Artificial sequence
<400> 133
TCAGCTCTTG AATGTTTGAA GACAG 25
<210> 134
<211> 25
<212> DNA
<213> Artificial sequence
<400> 134
CAGAGTTTGA CCCATTATGA GGATC 25
<210> 135
<211> 25
<212> DNA
<213> Artificial sequence
<400> 135
ACAAACTTGT CCTCATCAGG TGTAT 25
<210> 136
<211> 25
<212> DNA
<213> Artificial sequence
<400> 136
AAAAACCAGG TTTAGATGTA GCAGG 25
<210> 137
<211> 21
<212> DNA
<213> Artificial sequence
<400> 137
GATGAGGGGA CATAGCACCA C 21
<210> 138
<211> 25
<212> DNA
<213> Artificial sequence
<400> 138
GCAGATTATA CCATTCCCCC AAACT 25
<210> 139
<211> 25
<212> DNA
<213> Artificial sequence
<400> 139
TCAGATGTTG TTACGAATTA CCCGA 25
<210> 140
<211> 25
<212> DNA
<213> Artificial sequence
<400> 140
ACATTTTGTT TCTCTTGATG GCCAT 25
<210> 141
<211> 25
<212> DNA
<213> Artificial sequence
<400> 141
TGCATTAGAC CATCGTTTTT GACTC 25
<210> 142
<211> 25
<212> DNA
<213> Artificial sequence
<400> 142
TGTTTGATAC GGTAATCGAC TGAGT 25
<210> 143
<211> 25
<212> DNA
<213> Artificial sequence
<400> 143
AGGACTCATC ATATGATTCA AAGCA 25
<210> 144
<211> 25
<212> DNA
<213> Artificial sequence
<400> 144
TTCCCAAATA ATTACCGTGT TGTGG 25
<210> 145
<211> 25
<212> DNA
<213> Artificial sequence
<400> 145
GCACGTGTGT ATGAGACCGA TATAA 25
<210> 146
<211> 25
<212> DNA
<213> Artificial sequence
<400> 146
ATATATTGTG ACTCACCACC AGCTG 25
<210> 147
<211> 25
<212> DNA
<213> Artificial sequence
<400> 147
CAAATAAAGT GGAAATCCGG TGACA 25
<210> 148
<211> 25
<212> DNA
<213> Artificial sequence
<400> 148
CAGGTGCACT ATTCATTCCC AAATT 25
<210> 149
<211> 25
<212> DNA
<213> Artificial sequence
<400> 149
CAGCTGATTA TTGAACGAAA ACTGC 25
<210> 150
<211> 25
<212> DNA
<213> Artificial sequence
<400> 150
TTGCTACACC CACAATCATT TATGG 25
<210> 151
<211> 25
<212> DNA
<213> Artificial sequence
<400> 151
CAGTTAACGC GTTATAAACA AGGGA 25
<210> 152
<211> 25
<212> DNA
<213> Artificial sequence
<400> 152
GTTCGGGAAG TAGTCTCAAG GAATA 25
<210> 153
<211> 25
<212> DNA
<213> Artificial sequence
<400> 153
CCGGCAGGAA AATTATAATG CAGAT 25
<210> 154
<211> 25
<212> DNA
<213> Artificial sequence
<400> 154
TGAGACAATT TCTTTTCAGC CTCAC 25
<210> 155
<211> 24
<212> DNA
<213> Artificial sequence
<400> 155
CTGTCCTAGT GCTGCCCTTA AATT 24
<210> 156
<211> 25
<212> DNA
<213> Artificial sequence
<400> 156
AGATTGGGAT AGAAATGCAC AAAGC 25
<210> 157
<211> 25
<212> DNA
<213> Artificial sequence
<400> 157
CTTTTCTTAC ATCATTTGCT GCAGC 25
<210> 158
<211> 24
<212> DNA
<213> Artificial sequence
<400> 158
GAAATCCTGC AGAAGTGGTT ACTC 24
<210> 159
<211> 25
<212> DNA
<213> Artificial sequence
<400> 159
GCCTTCATCT ACGTCCATAT TCTCT 25
<210> 160
<211> 25
<212> DNA
<213> Artificial sequence
<400> 160
CGGAGTGCCT TTATAAGATT GGAAG 25
<210> 161
<211> 26
<212> DNA
<213> Artificial sequence
<400> 161
GCTCTGCCAG ATATTAGTAT TCCTCT 26
<210> 162
<211> 27
<212> DNA
<213> Artificial sequence
<400> 162
CAGCTAACTG TTTTAATTTC TTGCACA 27
<210> 163
<211> 25
<212> DNA
<213> Artificial sequence
<400> 163
TCTCATTTCA AAGCATTCCA AGGTG 25
<210> 164
<211> 25
<212> DNA
<213> Artificial sequence
<400> 164
CGGAAGTACG AAACTGAGAG TGTAT 25
<210> 165
<211> 25
<212> DNA
<213> Artificial sequence
<400> 165
TTTGCTATGA AGGCAGATTT CGATC 25
<210> 166
<211> 25
<212> DNA
<213> Artificial sequence
<400> 166
TTCAGTCCAT CCTGAATACC TCATC 25
<210> 167
<211> 25
<212> DNA
<213> Artificial sequence
<400> 167
ACAACAGGAA GAATCACGAT CTACA 25
<210> 168
<211> 25
<212> DNA
<213> Artificial sequence
<400> 168
GTGTCTGCAT CTAGGTACAA AACAC 25
<210> 169
<211> 25
<212> DNA
<213> Artificial sequence
<400> 169
CACAGCTTTG GCATTGATAT ATGGT 25
<210> 170
<211> 25
<212> DNA
<213> Artificial sequence
<400> 170
GTGAAATGCC AACTCACCTG AATAT 25
<210> 171
<211> 25
<212> DNA
<213> Artificial sequence
<400> 171
CTATTCTGAC CCAGGTCTTA CACAA 25
<210> 172
<211> 25
<212> DNA
<213> Artificial sequence
<400> 172
ATTGAAGACT TTTGCCTCTT GTCAC 25
<210> 173
<211> 25
<212> DNA
<213> Artificial sequence
<400> 173
CCATTCATAC CAGATTCTGA TTGCC 25
<210> 174
<211> 26
<212> DNA
<213> Artificial sequence
<400> 174
TCACTTGTAT ATTGCTTCTT GACAGC 26
<210> 175
<211> 25
<212> DNA
<213> Artificial sequence
<400> 175
TAACTCGTCC AGCTCATCAT CTAAC 25
<210> 176
<211> 25
<212> DNA
<213> Artificial sequence
<400> 176
ATCGTTATTG CTGCTTCTTT CTTTC 25
<210> 177
<211> 25
<212> DNA
<213> Artificial sequence
<400> 177
TAAGGTTGTC ATCGGGATTG TTTTT 25
<210> 178
<211> 25
<212> DNA
<213> Artificial sequence
<400> 178
GAAAGGTACC ACATTGACAA CTTGT 25
<210> 179
<211> 25
<212> DNA
<213> Artificial sequence
<400> 179
TATATTTCCA GAAAAACAAG CGCCA 25
<210> 180
<211> 25
<212> DNA
<213> Artificial sequence
<400> 180
TTTCATCTTC CACGCTTTCT ATTGG 25
<210> 181
<211> 25
<212> DNA
<213> Artificial sequence
<400> 181
TTGCCAGCAT CCGAATACGT ATATA 25
<210> 182
<211> 24
<212> DNA
<213> Artificial sequence
<400> 182
GAAAGTCACT TCATGGTAGC TGGA 24
<210> 183
<211> 25
<212> DNA
<213> Artificial sequence
<400> 183
CATCATCAAT AGACCCAACC TAGCT 25
<210> 184
<211> 25
<212> DNA
<213> Artificial sequence
<400> 184
ATTACAGAAT ATGGGTATTG CAGGC 25
<210> 185
<211> 25
<212> DNA
<213> Artificial sequence
<400> 185
TATTGACACA GAGGAAAGTG GAAGA 25
<210> 186
<211> 25
<212> DNA
<213> Artificial sequence
<400> 186
CGCAATAAGG GTATCGTTGG ATTAT 25
<210> 187
<211> 25
<212> DNA
<213> Artificial sequence
<400> 187
TGGACTGACA GGTCAATACT TTGAT 25
<210> 188
<211> 24
<212> DNA
<213> Artificial sequence
<400> 188
CATGCCAGAG TGGAAGGTAG ATAG 24
<210> 189
<211> 25
<212> DNA
<213> Artificial sequence
<400> 189
AAAAGAGAAT TTTGTCGGTG TTGGT 25
<210> 190
<211> 25
<212> DNA
<213> Artificial sequence
<400> 190
ATATCTATTC AAGCCCAACA TTCGC 25
<210> 191
<211> 25
<212> DNA
<213> Artificial sequence
<400> 191
AATTCAAAGG AGCTCTGTAA GGACT 25
<210> 192
<211> 23
<212> DNA
<213> Artificial sequence
<400> 192
GGGCCTTTCA GAAAAGCTTC TTC 23
<210> 193
<211> 23
<212> DNA
<213> Artificial sequence
<400> 193
TTTTTCCCTG ATTCGTCGTT TCG 23
<210> 194
<211> 25
<212> DNA
<213> Artificial sequence
<400> 194
TTCTGTGTGC GTATTTCGTG TGTTG 25
<210> 195
<211> 25
<212> DNA
<213> Artificial sequence
<400> 195
AATGCAGTAA TCTCGAGCAT GATTG 25
<210> 196
<211> 25
<212> DNA
<213> Artificial sequence
<400> 196
TCTGCATTCG GTCCTTAAAT TCATG 25
<210> 197
<211> 24
<212> DNA
<213> Artificial sequence
<400> 197
CGCGTAAGTA TCTTCTGAAG ATGC 24
<210> 198
<211> 25
<212> DNA
<213> Artificial sequence
<400> 198
TATCTTATCT GCACACCTTC TCACC 25
<210> 199
<211> 25
<212> DNA
<213> Artificial sequence
<400> 199
AGATTGTAAT CAGGTCTAGC AAGGC 25
<210> 200
<211> 25
<212> DNA
<213> Artificial sequence
<400> 200
CTCGTTGAAT TGGGTGTTCG TATAA 25
<210> 201
<211> 25
<212> DNA
<213> Artificial sequence
<400> 201
TATTTAATTC TATCGCGGGT CCAGT 25
<210> 202
<211> 25
<212> DNA
<213> Artificial sequence
<400> 202
TTCAGCCCTC TATGTAGCCA GTATA 25
<210> 203
<211> 22
<212> DNA
<213> Artificial sequence
<400> 203
AGTCATCGAT GGCTTGTCTA CC 22
<210> 204
<211> 25
<212> DNA
<213> Artificial sequence
<400> 204
CTTCAAGTTT TGCCAAGATT TGCAA 25
<210> 205
<211> 25
<212> DNA
<213> Artificial sequence
<400> 205
GATCCAGAGA ACATCAACAC TGTTC 25
<210> 206
<211> 25
<212> DNA
<213> Artificial sequence
<400> 206
CCTCAACCTC TCAATACCTT TTGGA 25
<210> 207
<211> 25
<212> DNA
<213> Artificial sequence
<400> 207
GCATGCACAG ATTATTGAGA GAGAA 25
<210> 208
<211> 25
<212> DNA
<213> Artificial sequence
<400> 208
CCTATGAGTG GCCTTTGAGC TATTA 25
<210> 209
<211> 25
<212> DNA
<213> Artificial sequence
<400> 209
TGACGGATGG AGAACTAATA CGATT 25
<210> 210
<211> 25
<212> DNA
<213> Artificial sequence
<400> 210
ATTTCAATCA GGCTGTAGAT GTACA 25
<210> 211
<211> 25
<212> DNA
<213> Artificial sequence
<400> 211
TTACAGCTGC TGTGCATTGT ATGTT 25
<210> 212
<211> 25
<212> DNA
<213> Artificial sequence
<400> 212
CAGTCCAGAG AATCTTATGC ATGTT 25
<210> 213
<211> 25
<212> DNA
<213> Artificial sequence
<400> 213
TTGGACAGCT TACTTCGATA CACAT 25
<210> 214
<211> 25
<212> DNA
<213> Artificial sequence
<400> 214
AATAGATACA GTGGTACCAT CGTCG 25
<210> 215
<211> 25
<212> DNA
<213> Artificial sequence
<400> 215
AGAAGCAAGG TTAGAGCATG AACTA 25
<210> 216
<211> 23
<212> DNA
<213> Artificial sequence
<400> 216
GTGCGGAACA TGTCTGTAAC TTG 23
<210> 217
<211> 25
<212> DNA
<213> Artificial sequence
<400> 217
ATTACTGAGA TGATGATGGT GTGGT 25
<210> 218
<211> 25
<212> DNA
<213> Artificial sequence
<400> 218
TACCGTCTGA GGTAGTATAT TCCCG 25
<210> 219
<211> 24
<212> DNA
<213> Artificial sequence
<400> 219
CTGTAGGTCG GTCTATGAAG CTTC 24
<210> 220
<211> 25
<212> DNA
<213> Artificial sequence
<400> 220
TCCCCATCTA TGATATCCTC ATCCT 25
<210> 221
<211> 25
<212> DNA
<213> Artificial sequence
<400> 221
CTTGCATACC AATATGGATG TGCAC 25
<210> 222
<211> 25
<212> DNA
<213> Artificial sequence
<400> 222
CACACGAAAC TTTTACTGAT GACGA 25
<210> 223
<211> 24
<212> DNA
<213> Artificial sequence
<400> 223
GAATTGTGGT CTTCAGGGTT GGAT 24
<210> 224
<211> 25
<212> DNA
<213> Artificial sequence
<400> 224
TGGAATTTGA CATCGGACAA AAAGC 25
<210> 225
<211> 25
<212> DNA
<213> Artificial sequence
<400> 225
TTCCAGAGGA CTTTTTGGCT TTTTC 25
<210> 226
<211> 25
<212> DNA
<213> Artificial sequence
<400> 226
GGGTTCTGAT GAAGCTTCTT TATCG 25
<210> 227
<211> 25
<212> DNA
<213> Artificial sequence
<400> 227
AAACGACCTA TGCCAAAACT GATTT 25
<210> 228
<211> 25
<212> DNA
<213> Artificial sequence
<400> 228
TGCAGTAACT CAGATTGGTA AGTCA 25
<210> 229
<211> 25
<212> DNA
<213> Artificial sequence
<400> 229
ACAAATTTCA GCTTCTTTCC CGAAT 25
<210> 230
<211> 24
<212> DNA
<213> Artificial sequence
<400> 230
GACATTCAGC TCCCTCATGA GTTA 24
<210> 231
<211> 25
<212> DNA
<213> Artificial sequence
<400> 231
AGGGAATGCT CATTTCAACG AAATT 25
<210> 232
<211> 25
<212> DNA
<213> Artificial sequence
<400> 232
GCAAGGACTT ACAGCCATCT TTAGA 25
<210> 233
<211> 25
<212> DNA
<213> Artificial sequence
<400> 233
CATCCTCTTT CCGTCTTGTG AAATG 25
<210> 234
<211> 25
<212> DNA
<213> Artificial sequence
<400> 234
CTTTGATATG TGGGTTCACG TCGAG 25
<210> 235
<211> 25
<212> DNA
<213> Artificial sequence
<400> 235
CCCACACTGA CTTGATATAG GAAGT 25
<210> 236
<211> 25
<212> DNA
<213> Artificial sequence
<400> 236
AGTCGAGGAG AGTGAAAGAA AAGAA 25
<210> 237
<211> 25
<212> DNA
<213> Artificial sequence
<400> 237
CTATACTTTT CCGTTCGCTC ATTCC 25
<210> 238
<211> 25
<212> DNA
<213> Artificial sequence
<400> 238
CATAGATGTA TGAAATGCTG TGGCA 25
<210> 239
<211> 26
<212> DNA
<213> Artificial sequence
<400> 239
GTCCTAACTA TTATCCTACA TCCCCC 26
<210> 240
<211> 25
<212> DNA
<213> Artificial sequence
<400> 240
TCTCGAAACC TGAGAAGTGA TTAGT 25
<210> 241
<211> 25
<212> DNA
<213> Artificial sequence
<400> 241
TCAAGTTCTT CGGGTGGATA CTTTC 25
<210> 242
<211> 22
<212> DNA
<213> Artificial sequence
<400> 242
CACCATCGCA TGTGAACGTA AC 22
<210> 243
<211> 25
<212> DNA
<213> Artificial sequence
<400> 243
TTCAAGTCCC ACTCTTGTCA TTTTG 25
<210> 244
<211> 25
<212> DNA
<213> Artificial sequence
<400> 244
GGAAAGATGC TCTGTAAAGA ACTGT 25
<210> 245
<211> 25
<212> DNA
<213> Artificial sequence
<400> 245
ACCAATATCA GAACCAGGTT GAGAT 25
<210> 246
<211> 25
<212> DNA
<213> Artificial sequence
<400> 246
AGCTATACAA ACTCCACATC CAACT 25
<210> 247
<211> 25
<212> DNA
<213> Artificial sequence
<400> 247
ACTGTGAAGA AGAGATTGAG CAATG 25
<210> 248
<211> 25
<212> DNA
<213> Artificial sequence
<400> 248
CTTAGGAAGC ATTGTGGGTA TTGAC 25
<210> 249
<211> 25
<212> DNA
<213> Artificial sequence
<400> 249
AGTCATCCAT TAGACCCATT TTCCT 25
<210> 250
<211> 25
<212> DNA
<213> Artificial sequence
<400> 250
TTACCAGCTC ACAACAAATT TCCTC 25
<210> 251
<211> 24
<212> DNA
<213> Artificial sequence
<400> 251
CATTGGAAAC TGCTTCTTGA GTGG 24
<210> 252
<211> 23
<212> DNA
<213> Artificial sequence
<400> 252
GTGAGTACCG AGTATGCAGA GAG 23
<210> 253
<211> 25
<212> DNA
<213> Artificial sequence
<400> 253
CGTGTTCTTC CAGATTCACT CATGA 25
<210> 254
<211> 25
<212> DNA
<213> Artificial sequence
<400> 254
GTTGGAGTCG CACCAGTAGA TGAAA 25
<210> 255
<211> 25
<212> DNA
<213> Artificial sequence
<400> 255
GCTAAAGCAT TGGGAAGATA TTGCA 25
<210> 256
<211> 25
<212> DNA
<213> Artificial sequence
<400> 256
GAGATTCAGA TTATCAGCAG GACCT 25
<210> 257
<211> 25
<212> DNA
<213> Artificial sequence
<400> 257
GGTTGTTTCT TGTTGACGTC CATAA 25
<210> 258
<211> 25
<212> DNA
<213> Artificial sequence
<400> 258
CAGCTATCTC GTCTCGTACA TAGTC 25
<210> 259
<211> 25
<212> DNA
<213> Artificial sequence
<400> 259
GTAGCTGTGA TCTGGCAAAT TGTAA 25
<210> 260
<211> 25
<212> DNA
<213> Artificial sequence
<400> 260
AGTCTTCAAC ACGTGGAAAC TTTTT 25
<210> 261
<211> 25
<212> DNA
<213> Artificial sequence
<400> 261
TGCTTGAACT TACTTGTACA GCTTG 25
<210> 262
<211> 25
<212> DNA
<213> Artificial sequence
<400> 262
CAATTGTTGT GTTGTACTCC TTGGA 25
<210> 263
<211> 25
<212> DNA
<213> Artificial sequence
<400> 263
TTGCTCTAGA TCAAGAGAAA GCCTA 25
<210> 264
<211> 25
<212> DNA
<213> Artificial sequence
<400> 264
AATTGCCATA CATATAGCAG GCATG 25
<210> 265
<211> 25
<212> DNA
<213> Artificial sequence
<400> 265
AAATGATTCT TAGAACGCGA TGGAC 25
<210> 266
<211> 25
<212> DNA
<213> Artificial sequence
<400> 266
CCAAAATGCC TTTCCTCATA CTCTC 25
<210> 267
<211> 25
<212> DNA
<213> Artificial sequence
<400> 267
AAGTTCACCA CTTAAAACCG GAAAG 25
<210> 268
<211> 21
<212> DNA
<213> Artificial sequence
<400> 268
AATTTTCTGC CCCCGACTCT C 21
<210> 269
<211> 25
<212> DNA
<213> Artificial sequence
<400> 269
AATCCATTCT TTGCTTTCAG TCCTG 25
<210> 270
<211> 25
<212> DNA
<213> Artificial sequence
<400> 270
GAGAAATGTG CATATAGTGT GCTGT 25
<210> 271
<211> 25
<212> DNA
<213> Artificial sequence
<400> 271
AATGACTGGG AGTACTTTGC ATTTC 25
<210> 272
<211> 25
<212> DNA
<213> Artificial sequence
<400> 272
GCAATGGATG TGGAATAAGA AGGTC 25
<210> 273
<211> 25
<212> DNA
<213> Artificial sequence
<400> 273
ATCTTTGAAC AGAACATGCA TAGCC 25
<210> 274
<211> 25
<212> DNA
<213> Artificial sequence
<400> 274
AATTTCCAGT CGTATCCCCA AAAAC 25
<210> 275
<211> 25
<212> DNA
<213> Artificial sequence
<400> 275
ATGTATCCCC TGGAAAGAGT GATTT 25
<210> 276
<211> 25
<212> DNA
<213> Artificial sequence
<400> 276
AATGTATGTT GCTAACAGCT AACCG 25
<210> 277
<211> 25
<212> DNA
<213> Artificial sequence
<400> 277
CAGAAAATCC GAACAACTTG AATGC 25
<210> 278
<211> 25
<212> DNA
<213> Artificial sequence
<400> 278
GTGTTGTGAT TTTCCTTCCT TCACT 25
<210> 279
<211> 25
<212> DNA
<213> Artificial sequence
<400> 279
CATCCTCTGC TTCGAAGATT TCTTG 25
<210> 280
<211> 24
<212> DNA
<213> Artificial sequence
<400> 280
CGGCTCTTCA AAAGCCATTC ATTA 24
<210> 281
<211> 25
<212> DNA
<213> Artificial sequence
<400> 281
CATGCATTAT CATCCAATCC CTCAC 25
<210> 282
<211> 25
<212> DNA
<213> Artificial sequence
<400> 282
CTGGAAGAAC ACACTAATCC TCTGA 25
<210> 283
<211> 25
<212> DNA
<213> Artificial sequence
<400> 283
CCACTTCCCA TTGCCATTTT AATCA 25
<210> 284
<211> 25
<212> DNA
<213> Artificial sequence
<400> 284
TAGACTACAT TGTTGACAGT GCGTA 25
<210> 285
<211> 25
<212> DNA
<213> Artificial sequence
<400> 285
CCTTGTTCAG TGACTAGGAA TCTGA 25
<210> 286
<211> 25
<212> DNA
<213> Artificial sequence
<400> 286
CCATTGAACT TCTTCACCTC TTTGT 25
<210> 287
<211> 25
<212> DNA
<213> Artificial sequence
<400> 287
GGCAATCTGT TAGAACTGCT ATCAC 25
<210> 288
<211> 25
<212> DNA
<213> Artificial sequence
<400> 288
AAAATCTGAA GGCGGAATGT TCAAT 25
<210> 289
<211> 24
<212> DNA
<213> Artificial sequence
<400> 289
GGTTGCATTC ATACCAGAAC ACAC 24
<210> 290
<211> 25
<212> DNA
<213> Artificial sequence
<400> 290
CGATTGTAAA TGTAGCTCCA ACTCC 25
<210> 291
<211> 25
<212> DNA
<213> Artificial sequence
<400> 291
GTCAAACTAG AGTCAAGCTC AACAG 25
<210> 292
<211> 25
<212> DNA
<213> Artificial sequence
<400> 292
CAGAAAGTGG TGTTGATGCA ATGTA 25
<210> 293
<211> 25
<212> DNA
<213> Artificial sequence
<400> 293
TCAACCTCTC GTTAAAGTGC ATAGA 25
<210> 294
<211> 25
<212> DNA
<213> Artificial sequence
<400> 294
GAGGGTCATG CAGGATATTC TCTTC 25
<210> 295
<211> 25
<212> DNA
<213> Artificial sequence
<400> 295
CTTCCTTCCA TGTACTTGAG TCTCA 25
<210> 296
<211> 25
<212> DNA
<213> Artificial sequence
<400> 296
CCCCCGTCTA AACTAATTAC CTGTT 25
<210> 297
<211> 25
<212> DNA
<213> Artificial sequence
<400> 297
AAATGGTGAC TATTTCGATC AACCC 25
<210> 298
<211> 25
<212> DNA
<213> Artificial sequence
<400> 298
TGTCGAGAAA GCATTTCAAA AAGGA 25
<210> 299
<211> 25
<212> DNA
<213> Artificial sequence
<400> 299
AATCTTGGGG GTGTATGTTT AACCT 25
<210> 300
<211> 25
<212> DNA
<213> Artificial sequence
<400> 300
CGAGTTGGTC CATAGTCATC ATTTC 25
<210> 301
<211> 25
<212> DNA
<213> Artificial sequence
<400> 301
AATTTCTCTG TTCGGATGAC AGGAA 25
<210> 302
<211> 25
<212> DNA
<213> Artificial sequence
<400> 302
TCCATATCTA CTTCGACATG GTCTT 25
<210> 303
<211> 25
<212> DNA
<213> Artificial sequence
<400> 303
AGCCTGAACA CAAAATTGAA ATTGA 25
<210> 304
<211> 25
<212> DNA
<213> Artificial sequence
<400> 304
TGCAGAGCAA GCTAATTTAA CTCTC 25
<210> 305
<211> 25
<212> DNA
<213> Artificial sequence
<400> 305
ATAGGGATGA GAGAAACAGA CCTTG 25
<210> 306
<211> 24
<212> DNA
<213> Artificial sequence
<400> 306
CTGATACTTC TGGGGAGGTT ACTG 24
<210> 307
<211> 25
<212> DNA
<213> Artificial sequence
<400> 307
ATATAATGTG ACACCGGCTT AATGC 25
<210> 308
<211> 25
<212> DNA
<213> Artificial sequence
<400> 308
GTGCGTTGAA AATGAACTTT GTTGA 25
<210> 309
<211> 25
<212> DNA
<213> Artificial sequence
<400> 309
ATTCGTGTAA TTCTAGATCT GGGGG 25
<210> 310
<211> 25
<212> DNA
<213> Artificial sequence
<400> 310
ACGATAAGTT GCTCCTCCAT AACAT 25
<210> 311
<211> 25
<212> DNA
<213> Artificial sequence
<400> 311
ATTGAATGTA TGACGGCAGA TTTCG 25
<210> 312
<211> 25
<212> DNA
<213> Artificial sequence
<400> 312
TGGCAGAAAT TTGATTGCAA GACTA 25
<210> 313
<211> 27
<212> DNA
<213> Artificial sequence
<400> 313
CGAACATTCA AGTAAAACTA CATTTGT 27
<210> 314
<211> 25
<212> DNA
<213> Artificial sequence
<400> 314
AGCACGCAAT CTCTAATTGT TCAAA 25
<210> 315
<211> 25
<212> DNA
<213> Artificial sequence
<400> 315
CGTGAAGTGG TTCAAGTTAC AAGTT 25
<210> 316
<211> 25
<212> DNA
<213> Artificial sequence
<400> 316
GAGATCATGT ACGATTGAAG GAGTG 25
<210> 317
<211> 25
<212> DNA
<213> Artificial sequence
<400> 317
GCAAAAGAAT GCGAAGATGG AAGTA 25
<210> 318
<211> 25
<212> DNA
<213> Artificial sequence
<400> 318
GCTTGTAGAT CGCTATGAGG AAGAA 25
<210> 319
<211> 23
<212> DNA
<213> Artificial sequence
<400> 319
GCTAGTTTGC ACCGCATATA CTG 23
<210> 320
<211> 25
<212> DNA
<213> Artificial sequence
<400> 320
TAGCGTGACC TCTAGTATGA TCTCT 25
<210> 321
<211> 26
<212> DNA
<213> Artificial sequence
<400> 321
GCTTGACATA CATAATCACT ATGGGC 26
<210> 322
<211> 25
<212> DNA
<213> Artificial sequence
<400> 322
CGTTGTTATG TCTGGTGCAT GATTT 25
<210> 323
<211> 25
<212> DNA
<213> Artificial sequence
<400> 323
GGAACAGTTC AGATGGAGTT TTGAG 25
<210> 324
<211> 25
<212> DNA
<213> Artificial sequence
<400> 324
CTGGTTTCAT CGAAGGAAGA AGATG 25
<210> 325
<211> 25
<212> DNA
<213> Artificial sequence
<400> 325
GTACATTCTT TGTCCTGATT CCTGC 25
<210> 326
<211> 25
<212> DNA
<213> Artificial sequence
<400> 326
TTGACCATGG TGAGTCGAAA AGAAG 25
<210> 327
<211> 25
<212> DNA
<213> Artificial sequence
<400> 327
GTCCTTGAGA GACATTACAT TGCTG 25
<210> 328
<211> 26
<212> DNA
<213> Artificial sequence
<400> 328
TCACAAGGTA AAGCATAGAT AAAGCA 26
<210> 329
<211> 25
<212> DNA
<213> Artificial sequence
<400> 329
GTTCACATCT TTTTCGTGCT TGTTC 25
<210> 330
<211> 25
<212> DNA
<213> Artificial sequence
<400> 330
GAAGGTGTGT GGATGTTTAG AGTTG 25
<210> 331
<211> 25
<212> DNA
<213> Artificial sequence
<400> 331
TAAAGGTTAC TTGACCATGG CTACA 25
<210> 332
<211> 25
<212> DNA
<213> Artificial sequence
<400> 332
GCTCAGACTA TGGGTAGGAG ATTTT 25
<210> 333
<211> 25
<212> DNA
<213> Artificial sequence
<400> 333
TACATCTCCA GAAGAGTGCT TTCAA 25
<210> 334
<211> 25
<212> DNA
<213> Artificial sequence
<400> 334
AGGTCAATCT TCAGAGTCAG TTTGA 25
<210> 335
<211> 25
<212> DNA
<213> Artificial sequence
<400> 335
TCAATTTCTC GTTTGCTTAC ACAAG 25
<210> 336
<211> 25
<212> DNA
<213> Artificial sequence
<400> 336
TCTACAGATG CAAATTTGAC ACACC 25
<210> 337
<211> 25
<212> DNA
<213> Artificial sequence
<400> 337
TCGTAGTCTG GACTTGTTCA TTCTT 25
<210> 338
<211> 25
<212> DNA
<213> Artificial sequence
<400> 338
TCTTGCGATT TTCACCTTGA ACTTC 25
<210> 339
<211> 25
<212> DNA
<213> Artificial sequence
<400> 339
TCTATACCTG ATGATCAAGC GTACC 25
<210> 340
<211> 25
<212> DNA
<213> Artificial sequence
<400> 340
TGGCATACAC TTCGAGATAA CTAGG 25
<210> 341
<211> 25
<212> DNA
<213> Artificial sequence
<400> 341
TGACGACGAT ACTATGAAGT TCCTC 25
<210> 342
<211> 25
<212> DNA
<213> Artificial sequence
<400> 342
CGGTGAAGGA AGACAAACTA AATGA 25
<210> 343
<211> 25
<212> DNA
<213> Artificial sequence
<400> 343
TGAGGTAACG TAGTGAAGTT CCAAT 25
<210> 344
<211> 25
<212> DNA
<213> Artificial sequence
<400> 344
GCCTAGTACA AGGATCCTTC TCATT 25
<210> 345
<211> 25
<212> DNA
<213> Artificial sequence
<400> 345
TTATACCCTT TACCGTCTTC CAACA 25
<210> 346
<211> 25
<212> DNA
<213> Artificial sequence
<400> 346
AAAAACCCTT CCAAAAACCC TTCTT 25
<210> 347
<211> 25
<212> DNA
<213> Artificial sequence
<400> 347
TTCCCAAGCT ATTATTGTTC TTGGT 25
<210> 348
<211> 25
<212> DNA
<213> Artificial sequence
<400> 348
TTTGCGAATT AATGGTCTGA ATTCG 25
<210> 349
<211> 25
<212> DNA
<213> Artificial sequence
<400> 349
TTTTGGCCAT CTTCTCCAAA CAAAT 25
<210> 350
<211> 25
<212> DNA
<213> Artificial sequence
<400> 350
ACGCTTACTC TTACATGGTC AGATT 25
<210> 351
<211> 25
<212> DNA
<213> Artificial sequence
<400> 351
CAGATCTGCT GTATTTGAAG TTGCA 25
<210> 352
<211> 25
<212> DNA
<213> Artificial sequence
<400> 352
GCAACATATT GATCAAGGCT CAAGA 25
<210> 353
<211> 25
<212> DNA
<213> Artificial sequence
<400> 353
AACTGGAAGA TTTGAACGAG GTCTA 25
<210> 354
<211> 25
<212> DNA
<213> Artificial sequence
<400> 354
CAAAGCAGTA GATTCATCGA GCAAT 25
<210> 355
<211> 25
<212> DNA
<213> Artificial sequence
<400> 355
AAGAGAATTA GCCAGTGCAT AAAGC 25
<210> 356
<211> 25
<212> DNA
<213> Artificial sequence
<400> 356
ATTAATTCTT GAGTGAGGCT GGTTG 25
<210> 357
<211> 25
<212> DNA
<213> Artificial sequence
<400> 357
ACAATCATCA ATGTGCTGAC TTTCA 25
<210> 358
<211> 25
<212> DNA
<213> Artificial sequence
<400> 358
ACGTGAACGG AGATATAAGA GTCAG 25
<210> 359
<211> 25
<212> DNA
<213> Artificial sequence
<400> 359
ACAGACAACA AGGAAGATTA CAAGC 25
<210> 360
<211> 25
<212> DNA
<213> Artificial sequence
<400> 360
GCCACGGATA AAGCCACTTA TGTTA 25
<210> 361
<211> 25
<212> DNA
<213> Artificial sequence
<400> 361
ACCGTCCAAG AGTACCTTTT CATTA 25
<210> 362
<211> 25
<212> DNA
<213> Artificial sequence
<400> 362
CTGATGTAGA TAAAGCCGGA GAGAA 25
<210> 363
<211> 25
<212> DNA
<213> Artificial sequence
<400> 363
ACGAATATCT ACACGTACGA AGAAA 25
<210> 364
<211> 25
<212> DNA
<213> Artificial sequence
<400> 364
TAGATGTTTT TGACGACAAG TTGGT 25
<210> 365
<211> 25
<212> DNA
<213> Artificial sequence
<400> 365
ACTGCCCAGT ACAATTCAAT TTGTT 25
<210> 366
<211> 25
<212> DNA
<213> Artificial sequence
<400> 366
ATGAAGAATG AACGACAGAG GAAGA 25
<210> 367
<211> 25
<212> DNA
<213> Artificial sequence
<400> 367
ACTTGTACAC ATTGTGGTAG ACAGA 25
<210> 368
<211> 25
<212> DNA
<213> Artificial sequence
<400> 368
GAAACCACAG CGTTTGAGAA AATTG 25
<210> 369
<211> 25
<212> DNA
<213> Artificial sequence
<400> 369
AGCCAAAGAA TGAAACTAGA GTGGT 25
<210> 370
<211> 25
<212> DNA
<213> Artificial sequence
<400> 370
CGCAATCACT GTATCCAGAT GATAG 25
<210> 371
<211> 25
<212> DNA
<213> Artificial sequence
<400> 371
AGGTCTAAAT CAGAAACTTG CCTCT 25
<210> 372
<211> 25
<212> DNA
<213> Artificial sequence
<400> 372
ATTCAGAAAA TTACTCTGTG ACGGG 25
<210> 373
<211> 25
<212> DNA
<213> Artificial sequence
<400> 373
AGGTTGGATA TAAGTGCAAT TCCAC 25
<210> 374
<211> 25
<212> DNA
<213> Artificial sequence
<400> 374
TTAAATTCAC GAGTCCTTCC ATCCT 25
<210> 375
<211> 25
<212> DNA
<213> Artificial sequence
<400> 375
AGTCTCAATA GCAGATGAAG CATGA 25
<210> 376
<211> 25
<212> DNA
<213> Artificial sequence
<400> 376
ATGGAATTTG AAGGGTTGGA GAAAC 25
<210> 377
<211> 24
<212> DNA
<213> Artificial sequence
<400> 377
ATATCACGCT GAGCTCTAGT CAAG 24
<210> 378
<211> 25
<212> DNA
<213> Artificial sequence
<400> 378
AAAGATTTCA AACCCGTCTC CTCTA 25
<210> 379
<211> 25
<212> DNA
<213> Artificial sequence
<400> 379
ATCGAGAGCT TCTTTTTGCT TCTTC 25
<210> 380
<211> 24
<212> DNA
<213> Artificial sequence
<400> 380
TACACTGTGC TTCAACGAAA AGTG 24
<210> 381
<211> 25
<212> DNA
<213> Artificial sequence
<400> 381
ATTAGATACA TACCTCCTGC TGTGG 25
<210> 382
<211> 25
<212> DNA
<213> Artificial sequence
<400> 382
TAATTGGGGG TCGCGTAATC TATTA 25
<210> 383
<211> 25
<212> DNA
<213> Artificial sequence
<400> 383
ATTCGATTGA AGACTCGACT GAAGA 25
<210> 384
<211> 25
<212> DNA
<213> Artificial sequence
<400> 384
TAATTAAAGT TCCAACGACT GGCAG 25
<210> 385
<211> 25
<212> DNA
<213> Artificial sequence
<400> 385
CAAAGTGAAT GGACTGAGAC AAGGA 25
<210> 386
<211> 25
<212> DNA
<213> Artificial sequence
<400> 386
TTTTAACTGG CTTTGCTGAT AGCTC 25
<210> 387
<211> 25
<212> DNA
<213> Artificial sequence
<400> 387
CAAGCAGCTC CTGTATCGTT ACTAT 25
<210> 388
<211> 25
<212> DNA
<213> Artificial sequence
<400> 388
TGTTACTGTC CCTTTCTTCG ACTTC 25
<210> 389
<211> 25
<212> DNA
<213> Artificial sequence
<400> 389
CAATACGACG TAAATTTCGA GCAGA 25
<210> 390
<211> 24
<212> DNA
<213> Artificial sequence
<400> 390
AAGTCACGTC CTGAAAGAAA CAGT 24
<210> 391
<211> 25
<212> DNA
<213> Artificial sequence
<400> 391
CAGTACACTG ACAGTACATC ACAGT 25
<210> 392
<211> 25
<212> DNA
<213> Artificial sequence
<400> 392
TTGTACCTGG TCTCCCTTAC ATTTT 25
<210> 393
<211> 23
<212> DNA
<213> Artificial sequence
<400> 393
CCACATTTCC ATCTCCTTGC ATC 23
<210> 394
<211> 25
<212> DNA
<213> Artificial sequence
<400> 394
TCAATCACCA GGGGTTCTTA GATTT 25
<210> 395
<211> 25
<212> DNA
<213> Artificial sequence
<400> 395
CCACGACCTG TAAGATTGAG AAAAG 25
<210> 396
<211> 25
<212> DNA
<213> Artificial sequence
<400> 396
ATTTTGCTCT CTTTGATTGT GGAGG 25
<210> 397
<211> 25
<212> DNA
<213> Artificial sequence
<400> 397
CCGATATCTA CCGGCATTTC TCAAT 25
<210> 398
<211> 25
<212> DNA
<213> Artificial sequence
<400> 398
CCACAGTTAT TCTAGGAGTG CTCTT 25
<210> 399
<211> 25
<212> DNA
<213> Artificial sequence
<400> 399
CCGGAAGTCT TCGGTTTAAA TGAAT 25
<210> 400
<211> 25
<212> DNA
<213> Artificial sequence
<400> 400
GACTCGGAAT CTGTTGTCAA TGTTC 25
<210> 401
<211> 25
<212> DNA
<213> Artificial sequence
<400> 401
CTCGAGTTGG GCTTTAATTG TACAT 25
<210> 402
<211> 25
<212> DNA
<213> Artificial sequence
<400> 402
ACATCTGGTT GATATCGTCC TCATC 25
<210> 403
<211> 25
<212> DNA
<213> Artificial sequence
<400> 403
GAACAAGGAG GACCAAACCA AATTT 25
<210> 404
<211> 25
<212> DNA
<213> Artificial sequence
<400> 404
AAGCATTCAT AGTCCCGTTT TCTTC 25
<210> 405
<211> 25
<212> DNA
<213> Artificial sequence
<400> 405
GAATGGATGT TCCTCCTTCA AGTGA 25
<210> 406
<211> 25
<212> DNA
<213> Artificial sequence
<400> 406
GCATTGATAC ACTCTTCTTC GAAGT 25
<210> 407
<211> 25
<212> DNA
<213> Artificial sequence
<400> 407
GAGCAAGATT CTGGTTAAAA AGCCT 25
<210> 408
<211> 25
<212> DNA
<213> Artificial sequence
<400> 408
CCAAACAAAA CCACTCGAAC TCTAT 25
<210> 409
<211> 25
<212> DNA
<213> Artificial sequence
<400> 409
GATCAGCAAC TTGTTGAGAA CAGAA 25
<210> 410
<211> 25
<212> DNA
<213> Artificial sequence
<400> 410
CTTCACACCT TCACAGTTCC TTGAA 25
<210> 411
<211> 25
<212> DNA
<213> Artificial sequence
<400> 411
GCCATGCTGC GATAGAAATG TTATA 25
<210> 412
<211> 24
<212> DNA
<213> Artificial sequence
<400> 412
GCGTTTGACG TTCTTTTTGT TCTC 24
<210> 413
<211> 27
<212> DNA
<213> Artificial sequence
<400> 413
GCTCAAGGTG TAAATACTGT ATTTCGT 27
<210> 414
<211> 25
<212> DNA
<213> Artificial sequence
<400> 414
GGACGAATGA CCATGATTTC GAAAT 25
<210> 415
<211> 25
<212> DNA
<213> Artificial sequence
<400> 415
GGTCTCAACG ACGTGAGATA CTTTA 25
<210> 416
<211> 25
<212> DNA
<213> Artificial sequence
<400> 416
AACAGAGCTA GTATTGTATC GCACT 25
<210> 417
<211> 25
<212> DNA
<213> Artificial sequence
<400> 417
GTATTCCCAA ACGATCCACG ATTTT 25
<210> 418
<211> 25
<212> DNA
<213> Artificial sequence
<400> 418
GAACGATGTG TATGCGTTTG CTATA 25
<210> 419
<211> 25
<212> DNA
<213> Artificial sequence
<400> 419
GTCGAAAGGC ATGATCTTCT TGATG 25
<210> 420
<211> 25
<212> DNA
<213> Artificial sequence
<400> 420
TCGTCGAATT CTCCTTCTTT AATCT 25
<210> 421
<211> 25
<212> DNA
<213> Artificial sequence
<400> 421
GTGTATCGCG AGGAAGAAGA ATAAC 25
<210> 422
<211> 25
<212> DNA
<213> Artificial sequence
<400> 422
CCGAAATGAA CCGAAATTTG GAAAA 25
<210> 423
<211> 24
<212> DNA
<213> Artificial sequence
<400> 423
GTGTTGAGGA CCCATTTGTT ACTG 24
<210> 424
<211> 25
<212> DNA
<213> Artificial sequence
<400> 424
GAGAGGTCTC GTTGTATGTC GACTC 25
<210> 425
<211> 25
<212> DNA
<213> Artificial sequence
<400> 425
GTTTCCTCTT CCGTTAAAAG GGTTG 25
<210> 426
<211> 25
<212> DNA
<213> Artificial sequence
<400> 426
GATGGCTGTC AATTTGGGTA CATAG 25
<210> 427
<211> 25
<212> DNA
<213> Artificial sequence
<400> 427
TAAGGTTCTA CTCGTTCAAA TGTGA 25
<210> 428
<211> 25
<212> DNA
<213> Artificial sequence
<400> 428
GAATCCATCT CTCATCACAG CAACT 25
<210> 429
<211> 25
<212> DNA
<213> Artificial sequence
<400> 429
TACTATCTTT CGGCTTATGG CTTGT 25
<210> 430
<211> 25
<212> DNA
<213> Artificial sequence
<400> 430
CGTGAAATTT AGGAACAGCC GATAA 25
<210> 431
<211> 25
<212> DNA
<213> Artificial sequence
<400> 431
TAGAATGTTG AGTCCCGATC AAGTT 25
<210> 432
<211> 23
<212> DNA
<213> Artificial sequence
<400> 432
GGATCCAGCT ACCGAAACAG AAG 23
<210> 433
<211> 25
<212> DNA
<213> Artificial sequence
<400> 433
TATAGACCAG AGCTGTTTGT CATGT 25
<210> 434
<211> 24
<212> DNA
<213> Artificial sequence
<400> 434
CTAAGCATGG TCGAAGTGTC AGTA 24
<210> 435
<211> 25
<212> DNA
<213> Artificial sequence
<400> 435
TATATTGGTG GTCAGTCTTC CCTTC 25
<210> 436
<211> 25
<212> DNA
<213> Artificial sequence
<400> 436
CCAGATCGGA AAATATGAAT CGGAC 25
<210> 437
<211> 25
<212> DNA
<213> Artificial sequence
<400> 437
TCAAAGTCAT TTCGCATTCA GAACA 25
<210> 438
<211> 24
<212> DNA
<213> Artificial sequence
<400> 438
GCAAGGAGAA CAACAAGGAA AAGA 24
<210> 439
<211> 25
<212> DNA
<213> Artificial sequence
<400> 439
TCAACCAACT TCGACTCTTA GAGTT 25
<210> 440
<211> 25
<212> DNA
<213> Artificial sequence
<400> 440
TGATTATTTC CGGATCTAGC AGGAT 25
<210> 441
<211> 25
<212> DNA
<213> Artificial sequence
<400> 441
TCACAGAAAT TGCAAAGTAT ACCGC 25
<210> 442
<211> 25
<212> DNA
<213> Artificial sequence
<400> 442
TTCCTGTATC TCCCGTATTT GAACA 25
<210> 443
<211> 25
<212> DNA
<213> Artificial sequence
<400> 443
TCCAATTTCA CTTCCAATCG ATGTG 25
<210> 444
<211> 25
<212> DNA
<213> Artificial sequence
<400> 444
GATATCGAAC CAGACGAAAT TCCTG 25
<210> 445
<211> 25
<212> DNA
<213> Artificial sequence
<400> 445
TCGTTGGATA GCATTCTCTC ATTGA 25
<210> 446
<211> 24
<212> DNA
<213> Artificial sequence
<400> 446
TGCAATACAC GAGGTCGAGA ATAG 24
<210> 447
<211> 25
<212> DNA
<213> Artificial sequence
<400> 447
TTCTTCTTGG ATAAACCTGT CTGAG 25
<210> 448
<211> 25
<212> DNA
<213> Artificial sequence
<400> 448
GGTTGTGAGA TAATCAGTGT TGGTC 25
<210> 449
<211> 22
<212> DNA
<213> Artificial sequence
<400> 449
TTGTTGGGAT TGGGAAGGAG AC 22
<210> 450
<211> 21
<212> DNA
<213> Artificial sequence
<400> 450
ACGAGATGAG GAGCATATTG C 21
<210> 451
<211> 25
<212> DNA
<213> Artificial sequence
<400> 451
AAAAAGAAAG AGGCATGCAG TCTTT 25
<210> 452
<211> 22
<212> DNA
<213> Artificial sequence
<400> 452
TTCACGGCCT GTTTCATCAG TT 22
<210> 453
<211> 23
<212> DNA
<213> Artificial sequence
<400> 453
AAAAAGGGCA TCTGGGAACG TTA 23
<210> 454
<211> 25
<212> DNA
<213> Artificial sequence
<400> 454
AAACATGAGC CGAAATAGGT AGTCT 25
<210> 455
<211> 21
<212> DNA
<213> Artificial sequence
<400> 455
AAAAATAGAA CGCGACGCCT G 21
<210> 456
<211> 25
<212> DNA
<213> Artificial sequence
<400> 456
ACGTTTGAAA TACCGCCTTC ATAAT 25
<210> 457
<211> 25
<212> DNA
<213> Artificial sequence
<400> 457
AAACACTCCT TTTGTTTCTC AACGT 25
<210> 458
<211> 25
<212> DNA
<213> Artificial sequence
<400> 458
GTATTTTGGC ATTGTGACGT TGTTC 25
<210> 459
<211> 25
<212> DNA
<213> Artificial sequence
<400> 459
AAACCTACCA TTTGTCGATC AGGTA 25
<210> 460
<211> 24
<212> DNA
<213> Artificial sequence
<400> 460
GAAGCTTCCT GTTGTTAAAC GAGG 24
<210> 461
<211> 25
<212> DNA
<213> Artificial sequence
<400> 461
AAACTGCAGC AACCTTTCAG ATTAA 25
<210> 462
<211> 25
<212> DNA
<213> Artificial sequence
<400> 462
TCTACTGTTG GTGTTTTGGA AATCG 25
<210> 463
<211> 25
<212> DNA
<213> Artificial sequence
<400> 463
AAAGAACACA CACGAGTTCA TTCTC 25
<210> 464
<211> 25
<212> DNA
<213> Artificial sequence
<400> 464
CGCAGATGGA ATACGTATTC TCTCA 25
<210> 465
<211> 25
<212> DNA
<213> Artificial sequence
<400> 465
AAATGATACA TATGCGACCA TGACG 25
<210> 466
<211> 25
<212> DNA
<213> Artificial sequence
<400> 466
CTTTGTGAAA TGCTTTGATT CCACG 25
<210> 467
<211> 25
<212> DNA
<213> Artificial sequence
<400> 467
AAATGCATGT CTTGGTTGTT TTGTG 25
<210> 468
<211> 25
<212> DNA
<213> Artificial sequence
<400> 468
GATGCGCATA CACTCATTCA AATCT 25
<210> 469
<211> 25
<212> DNA
<213> Artificial sequence
<400> 469
AACGTAGCCG GGAAAATTAA CGTAT 25
<210> 470
<211> 25
<212> DNA
<213> Artificial sequence
<400> 470
CGACATCTCC TCCAACCATT TTTAG 25
<210> 471
<211> 25
<212> DNA
<213> Artificial sequence
<400> 471
AACTGAGAAT GTCTGAATCA CTGGA 25
<210> 472
<211> 23
<212> DNA
<213> Artificial sequence
<400> 472
TTCCGTGGAA GATTTTGTAC CGG 23
<210> 473
<211> 25
<212> DNA
<213> Artificial sequence
<400> 473
AAGATAACAA CATGCTTCGG AGATG 25
<210> 474
<211> 25
<212> DNA
<213> Artificial sequence
<400> 474
CTCGCCGATA AAATTTCAGG ACAAA 25
<210> 475
<211> 25
<212> DNA
<213> Artificial sequence
<400> 475
AAGATTCAAC AGTACAGTAG GTGGG 25
<210> 476
<211> 23
<212> DNA
<213> Artificial sequence
<400> 476
TGACGAGGAG GAAAAGCAGA TAG 23
<210> 477
<211> 25
<212> DNA
<213> Artificial sequence
<400> 477
AAGATTTCAA TATCGTCCTT GGCAC 25
<210> 478
<211> 25
<212> DNA
<213> Artificial sequence
<400> 478
CATGTGCTCG AATTATTCAG GAACA 25
<210> 479
<211> 25
<212> DNA
<213> Artificial sequence
<400> 479
AAGCAAGTAT GTATGCGTTC GTATG 25
<210> 480
<211> 25
<212> DNA
<213> Artificial sequence
<400> 480
ATTGAACGGA TGACCTTGAG GAAAT 25
<210> 481
<211> 24
<212> DNA
<213> Artificial sequence
<400> 481
AATCAGCTGC AACTCTTCGA GAAT 24
<210> 482
<211> 25
<212> DNA
<213> Artificial sequence
<400> 482
ACTCGGACTT TGAAATTTTG GATGA 25
<210> 483
<211> 25
<212> DNA
<213> Artificial sequence
<400> 483
AATGCAAAAT GTCGGATGAT CAAGA 25
<210> 484
<211> 25
<212> DNA
<213> Artificial sequence
<400> 484
AAGTTTTCTG CGTTTGTTTA TTGGC 25
<210> 485
<211> 25
<212> DNA
<213> Artificial sequence
<400> 485
AATTATTTTA GGTGGATGGG CGCTC 25
<210> 486
<211> 25
<212> DNA
<213> Artificial sequence
<400> 486
TGGAGGAGAC ACCATCTGAT ACTAA 25
<210> 487
<211> 25
<212> DNA
<213> Artificial sequence
<400> 487
ACAAAGCAAG AAATCACTGG AAGAG 25
<210> 488
<211> 25
<212> DNA
<213> Artificial sequence
<400> 488
CTGAACTCCA TCAACAAATT GGCAG 25
<210> 489
<211> 25
<212> DNA
<213> Artificial sequence
<400> 489
ACAATGAGGC AATAGCAGAA TTGAA 25
<210> 490
<211> 25
<212> DNA
<213> Artificial sequence
<400> 490
TCGATGACAC CAATCTGGAA GAATA 25
<210> 491
<211> 25
<212> DNA
<213> Artificial sequence
<400> 491
ACACCCTTTA ATGCTCAACT TTGAT 25
<210> 492
<211> 25
<212> DNA
<213> Artificial sequence
<400> 492
ACATGCCAGT AAATTCTCTA GTCCA 25
<210> 493
<211> 26
<212> DNA
<213> Artificial sequence
<400> 493
ACAGAACCAG ATAAGATACT ATGGCA 26
<210> 494
<211> 25
<212> DNA
<213> Artificial sequence
<400> 494
TGCTGTGTGA AGCTTAGTCC TATAA 25
<210> 495
<211> 24
<212> DNA
<213> Artificial sequence
<400> 495
ACAGGAAGGT TTACAAGCCA AGAA 24
<210> 496
<211> 25
<212> DNA
<213> Artificial sequence
<400> 496
ACAGAGAGTA GCTCCACTCT ATCTC 25
<210> 497
<211> 25
<212> DNA
<213> Artificial sequence
<400> 497
ACCAACCAAA ATCGTTAGAC CTTTC 25
<210> 498
<211> 25
<212> DNA
<213> Artificial sequence
<400> 498
GTTCCAGGTG TGTTATAGCA TTCTG 25
<210> 499
<211> 25
<212> DNA
<213> Artificial sequence
<400> 499
ACCACGGAAA GGTATAAGAC ATCAA 25
<210> 500
<211> 25
<212> DNA
<213> Artificial sequence
<400> 500
GATAGGAACA GCAAGAGCAG AAGAA 25
<210> 501
<211> 25
<212> DNA
<213> Artificial sequence
<400> 501
ACCAGTAGTA CAGATTCAGC TTCTG 25
<210> 502
<211> 24
<212> DNA
<213> Artificial sequence
<400> 502
TAGAACTAGT GACGGAGGCT GAAT 24
<210> 503
<211> 25
<212> DNA
<213> Artificial sequence
<400> 503
ACTAGGATTT TAGTCGATTT GGGCT 25
<210> 504
<211> 25
<212> DNA
<213> Artificial sequence
<400> 504
AATGTAGCGT CGTTCAATGT TTCTT 25
<210> 505
<211> 25
<212> DNA
<213> Artificial sequence
<400> 505
ACTGATCCTA CAGATTTGCT TGTCT 25
<210> 506
<211> 25
<212> DNA
<213> Artificial sequence
<400> 506
GATTTCACCA CACATTATCT GCCAA 25
<210> 507
<211> 25
<212> DNA
<213> Artificial sequence
<400> 507
AGAAACAAGC TGCACGAAAA TCTAT 25
<210> 508
<211> 25
<212> DNA
<213> Artificial sequence
<400> 508
GATCAGAAAG TGACAATTGA CGCTT 25
<210> 509
<211> 25
<212> DNA
<213> Artificial sequence
<400> 509
AGAAGGGAAG AGTACTGGTC TATCA 25
<210> 510
<211> 25
<212> DNA
<213> Artificial sequence
<400> 510
TATGTTATTT CCCAGAGCGT ACAGT 25
<210> 511
<211> 25
<212> DNA
<213> Artificial sequence
<400> 511
AGACAAGATA CGAGTGCTTA TTCCA 25
<210> 512
<211> 25
<212> DNA
<213> Artificial sequence
<400> 512
TACGACCATC CAGTAGTAAT GATGT 25
<210> 513
<211> 25
<212> DNA
<213> Artificial sequence
<400> 513
AGAGAGGGAA ATGTATCAGA GTTCG 25
<210> 514
<211> 24
<212> DNA
<213> Artificial sequence
<400> 514
CTGTTGTCGG AACCTTTGGA AAAA 24
<210> 515
<211> 23
<212> DNA
<213> Artificial sequence
<400> 515
AGCAACATCG TATGCTGTCT CTC 23
<210> 516
<211> 25
<212> DNA
<213> Artificial sequence
<400> 516
TCAGGTATCT TTGTGAAGAA GGAGG 25
<210> 517
<211> 25
<212> DNA
<213> Artificial sequence
<400> 517
AGCGTGTATA GCCTCAATTC TAGTT 25
<210> 518
<211> 25
<212> DNA
<213> Artificial sequence
<400> 518
TTCTTGAAAA ATCGCGGAGG AACAA 25
<210> 519
<211> 25
<212> DNA
<213> Artificial sequence
<400> 519
AGCTCAACAT GAACTGGAAT GAAAG 25
<210> 520
<211> 25
<212> DNA
<213> Artificial sequence
<400> 520
GACGTCAACG AGAAAATTGA AGAGT 25
<210> 521
<211> 25
<212> DNA
<213> Artificial sequence
<400> 521
AGGCTTACTT TGTCTACAGT CTCTG 25
<210> 522
<211> 25
<212> DNA
<213> Artificial sequence
<400> 522
TTACTCTTAC CGATTACGTG TCCAG 25
<210> 523
<211> 25
<212> DNA
<213> Artificial sequence
<400> 523
AGGGGAAGAA AATCAAGAGG CATAT 25
<210> 524
<211> 25
<212> DNA
<213> Artificial sequence
<400> 524
GTTACCAGCG AAATTTCCGT TTAAC 25
<210> 525
<211> 25
<212> DNA
<213> Artificial sequence
<400> 525
AGTATAACTC GAGGCTAGCG AAAAT 25
<210> 526
<211> 25
<212> DNA
<213> Artificial sequence
<400> 526
TCAATGATTA GCAAAAAGAG CGAGA 25
<210> 527
<211> 26
<212> DNA
<213> Artificial sequence
<400> 527
AGTGTCATCC ATAATCTCAA TTTCGT 26
<210> 528
<211> 19
<212> DNA
<213> Artificial sequence
<400> 528
TATCCCTGCT TTGGCCGAC 19
<210> 529
<211> 19
<212> DNA
<213> Artificial sequence
<400> 529
AGTTGGCAGC ACTGTCCAG 19
<210> 530
<211> 25
<212> DNA
<213> Artificial sequence
<400> 530
GAAGACTCAC ACTGCTTTCA AGAGT 25
<210> 531
<211> 25
<212> DNA
<213> Artificial sequence
<400> 531
AGTTTGGTCG ATGATCTCCA TTCTA 25
<210> 532
<211> 25
<212> DNA
<213> Artificial sequence
<400> 532
AACTTGGTAA GGTTCTCGCT AAGAA 25
<210> 533
<211> 25
<212> DNA
<213> Artificial sequence
<400> 533
ATACATGCTC ACTATTGCTT CTTGC 25
<210> 534
<211> 25
<212> DNA
<213> Artificial sequence
<400> 534
TAAAAGCGCT TTAATTACCG CTAGG 25
<210> 535
<211> 25
<212> DNA
<213> Artificial sequence
<400> 535
ATACGCAAAA CTCCAAAGGC AATAA 25
<210> 536
<211> 25
<212> DNA
<213> Artificial sequence
<400> 536
GTTGATTGAA TAAGGCGCAC TTTTT 25
<210> 537
<211> 25
<212> DNA
<213> Artificial sequence
<400> 537
ATAGAATCGT GTATGGAGGT TGCAT 25
<210> 538
<211> 25
<212> DNA
<213> Artificial sequence
<400> 538
TACCATGAAC CATCCACTAT GCAAG 25
<210> 539
<211> 25
<212> DNA
<213> Artificial sequence
<400> 539
ATAGCCAAGT TTTTGAGAGG AGGAA 25
<210> 540
<211> 25
<212> DNA
<213> Artificial sequence
<400> 540
TAAATAAACA AGCTGTGAAC CTGGC 25
<210> 541
<211> 25
<212> DNA
<213> Artificial sequence
<400> 541
ATAGTGCCAT TCTCATTACC CCTTT 25
<210> 542
<211> 24
<212> DNA
<213> Artificial sequence
<400> 542
ACTCGCAAAT GTTAATTTCG CAGA 24
<210> 543
<211> 25
<212> DNA
<213> Artificial sequence
<400> 543
ATATCAAAGA CGGAAAGGGA GTGAA 25
<210> 544
<211> 25
<212> DNA
<213> Artificial sequence
<400> 544
GCATGGAGTA GGTTCCTTGA TTCTA 25
<210> 545
<211> 25
<212> DNA
<213> Artificial sequence
<400> 545
ATATGCTTTT GAGTTCGGAG ACCTT 25
<210> 546
<211> 25
<212> DNA
<213> Artificial sequence
<400> 546
AATACCTGCA CAAAAGAAGA ACTCG 25
<210> 547
<211> 23
<212> DNA
<213> Artificial sequence
<400> 547
ATATTATGTC TCCCGAGTTC CGC 23
<210> 548
<211> 25
<212> DNA
<213> Artificial sequence
<400> 548
ACCAAGATAT GCAGATCTTT GAAGC 25
<210> 549
<211> 25
<212> DNA
<213> Artificial sequence
<400> 549
ATCACAAGAG CACTTTCACT CTGAT 25
<210> 550
<211> 25
<212> DNA
<213> Artificial sequence
<400> 550
GATAGTCAGA CCTCAGAGGA TGAAC 25
<210> 551
<211> 24
<212> DNA
<213> Artificial sequence
<400> 551
ATCACACGAC CATGGAAGAA AAAC 24
<210> 552
<211> 25
<212> DNA
<213> Artificial sequence
<400> 552
ATCGTCTGTT GAACCTGGCT GATAT 25
<210> 553
<211> 25
<212> DNA
<213> Artificial sequence
<400> 553
ATCATGTGTC CCTTCACGTA ATACT 25
<210> 554
<211> 25
<212> DNA
<213> Artificial sequence
<400> 554
TAGAGGCTGA TAGAGGACGA GATTA 25
<210> 555
<211> 25
<212> DNA
<213> Artificial sequence
<400> 555
ATCTTTTGCG GGACTAATGC TCATC 25
<210> 556
<211> 25
<212> DNA
<213> Artificial sequence
<400> 556
TATATTCAGG AGGTCTACCG CGATT 25
<210> 557
<211> 21
<212> DNA
<213> Artificial sequence
<400> 557
ATGGGGTATG CCTCCGATGA T 21
<210> 558
<211> 24
<212> DNA
<213> Artificial sequence
<400> 558
CAAACTCGTC AATGACATCC ATCC 24
<210> 559
<211> 25
<212> DNA
<213> Artificial sequence
<400> 559
ATGGTGGTAT GCACTCTTGA TTATT 25
<210> 560
<211> 24
<212> DNA
<213> Artificial sequence
<400> 560
GTTGGCGAAT GTACAGAATG AGTC 24
<210> 561
<211> 25
<212> DNA
<213> Artificial sequence
<400> 561
ATTCAGCTTC CCAACCTCTA TTCTT 25
<210> 562
<211> 25
<212> DNA
<213> Artificial sequence
<400> 562
CTAGTCGGAA CCAATCAGCT CAAAT 25
<210> 563
<211> 25
<212> DNA
<213> Artificial sequence
<400> 563
ATTCATGGAA AGTACGCGAC TAGAT 25
<210> 564
<211> 24
<212> DNA
<213> Artificial sequence
<400> 564
GAGCTCTTCG TCAGAATCTT GTTG 24
<210> 565
<211> 25
<212> DNA
<213> Artificial sequence
<400> 565
ATTGCACAAT TTTCCGAGGC TTTAT 25
<210> 566
<211> 22
<212> DNA
<213> Artificial sequence
<400> 566
GAGCTCCTGT ACACTGGAAA CA 22
<210> 567
<211> 25
<212> DNA
<213> Artificial sequence
<400> 567
ATTTACAGGA AATCGAGCGT CTCTA 25
<210> 568
<211> 25
<212> DNA
<213> Artificial sequence
<400> 568
AAATCCAGTT CTGCACAAGT TCATT 25
<210> 569
<211> 25
<212> DNA
<213> Artificial sequence
<400> 569
ATTTACATGC CTTTCCATGC TTTCT 25
<210> 570
<211> 25
<212> DNA
<213> Artificial sequence
<400> 570
TTTTGGTACC AGCGGCTAAA ATCTA 25
<210> 571
<211> 25
<212> DNA
<213> Artificial sequence
<400> 571
ATTTTCCTAG GACTGGACAT GTACC 25
<210> 572
<211> 25
<212> DNA
<213> Artificial sequence
<400> 572
TCTTTCTGCC ACATTTTCGA TGATC 25
<210> 573
<211> 25
<212> DNA
<213> Artificial sequence
<400> 573
CAAATTGCGG AAAGTTATCC TGACT 25
<210> 574
<211> 26
<212> DNA
<213> Artificial sequence
<400> 574
TCGTCTACGA CATCTTTAAT TACCAC 26
<210> 575
<211> 24
<212> DNA
<213> Artificial sequence
<400> 575
CAACATCTTC TCGACACTTT TCGG 24
<210> 576
<211> 25
<212> DNA
<213> Artificial sequence
<400> 576
CTCAACATGA TCCGACTCCA GAAAG 25
<210> 577
<211> 24
<212> DNA
<213> Artificial sequence
<400> 577
CAACCATCTG AGTGACAGGT AGAC 24
<210> 578
<211> 25
<212> DNA
<213> Artificial sequence
<400> 578
TCGCCTCCAC AGTATTCAAT CTATT 25
<210> 579
<211> 25
<212> DNA
<213> Artificial sequence
<400> 579
CAAGAACAAG AACAAGACAG ACGAA 25
<210> 580
<211> 25
<212> DNA
<213> Artificial sequence
<400> 580
TTGAGCTTGT CGACACATCT TTAAC 25
<210> 581
<211> 24
<212> DNA
<213> Artificial sequence
<400> 581
CAAGGAAGGT TGCGTTTTTG TTTG 24
<210> 582
<211> 25
<212> DNA
<213> Artificial sequence
<400> 582
GCAAATCTCC GGAATGTCGA TTATG 25
<210> 583
<211> 25
<212> DNA
<213> Artificial sequence
<400> 583
CAAGTACATA TCACCTTTGG CCTTG 25
<210> 584
<211> 23
<212> DNA
<213> Artificial sequence
<400> 584
CTTAATGGCA CCTCAGTTGA CAC 23
<210> 585
<211> 25
<212> DNA
<213> Artificial sequence
<400> 585
CAAGTCATAT TGTGTTCCTA TCGCA 25
<210> 586
<211> 25
<212> DNA
<213> Artificial sequence
<400> 586
GTCTGATCTT GGAATAACAG TTCGC 25
<210> 587
<211> 25
<212> DNA
<213> Artificial sequence
<400> 587
CAAGTCTCCA TGATTAAGGG CTACC 25
<210> 588
<211> 24
<212> DNA
<213> Artificial sequence
<400> 588
TGGTAGTCAC CCATCCTGTG TAAT 24
<210> 589
<211> 24
<212> DNA
<213> Artificial sequence
<400> 589
CAATAGAATT GTTCAGGGCT ACGC 24
<210> 590
<211> 25
<212> DNA
<213> Artificial sequence
<400> 590
CATCGTAATA GCAAATGGGT AAGCA 25
<210> 591
<211> 22
<212> DNA
<213> Artificial sequence
<400> 591
CAATGGGGAG ATGGATACCC TG 22
<210> 592
<211> 25
<212> DNA
<213> Artificial sequence
<400> 592
TTATGTGAAG TTGCGGTAAG GTTTC 25
<210> 593
<211> 24
<212> DNA
<213> Artificial sequence
<400> 593
CACCAGGCAA AGTTTCGACT TTAC 24
<210> 594
<211> 25
<212> DNA
<213> Artificial sequence
<400> 594
TTTCGTTCAC GGTATGCTTT GTTAA 25
<210> 595
<211> 25
<212> DNA
<213> Artificial sequence
<400> 595
CACGAACAGT CTAAAATCTG GCTTC 25
<210> 596
<211> 25
<212> DNA
<213> Artificial sequence
<400> 596
ACGAACAACA TGTTGAATGA CAGAA 25
<210> 597
<211> 23
<212> DNA
<213> Artificial sequence
<400> 597
CACTACTGCC CAGCTTTTTG ATG 23
<210> 598
<211> 25
<212> DNA
<213> Artificial sequence
<400> 598
AGTCATGTGA GAATACTCCA ACTCG 25
<210> 599
<211> 25
<212> DNA
<213> Artificial sequence
<400> 599
CACTCGTACA CTGTGTTCAA CTATG 25
<210> 600
<211> 25
<212> DNA
<213> Artificial sequence
<400> 600
CTCTTACTAA GCTAGCATCG CACAG 25
<210> 601
<211> 25
<212> DNA
<213> Artificial sequence
<400> 601
CACTGTTTGG TAAATCGTCG TATGT 25
<210> 602
<211> 25
<212> DNA
<213> Artificial sequence
<400> 602
CTCGTTTCGG TTCTGAGAAT GTAAG 25
<210> 603
<211> 23
<212> DNA
<213> Artificial sequence
<400> 603
CACTTGGCAT AATGACGAGC AAC 23
<210> 604
<211> 25
<212> DNA
<213> Artificial sequence
<400> 604
CTCAAAAACT AGGGCCCCAC TTTTC 25
<210> 605
<211> 25
<212> DNA
<213> Artificial sequence
<400> 605
CAGGAATCCG ACTATTTCAC CAAAG 25
<210> 606
<211> 25
<212> DNA
<213> Artificial sequence
<400> 606
GTCCCCCAAA GAATGTATCC TTTTG 25
<210> 607
<211> 25
<212> DNA
<213> Artificial sequence
<400> 607
CAGTTTTACT GGTACGAGAT TTGCA 25
<210> 608
<211> 25
<212> DNA
<213> Artificial sequence
<400> 608
CATGGTCAAA ATCGTGGTGA TCTTG 25
<210> 609
<211> 24
<212> DNA
<213> Artificial sequence
<400> 609
CATATCACCC ACGTCTTCAA CAAC 24
<210> 610
<211> 25
<212> DNA
<213> Artificial sequence
<400> 610
GTATCGCATA ATTTTACCGA GGTGG 25
<210> 611
<211> 25
<212> DNA
<213> Artificial sequence
<400> 611
CATCAGCACC GAAAGACTAA AAAGG 25
<210> 612
<211> 25
<212> DNA
<213> Artificial sequence
<400> 612
AACGACGACA GCAGATGTAG TAATA 25
<210> 613
<211> 25
<212> DNA
<213> Artificial sequence
<400> 613
CATCGTCGAT CCGTGAGTAC ATCTC 25
<210> 614
<211> 25
<212> DNA
<213> Artificial sequence
<400> 614
ATCTTGAATC TGTGGCTATC TCTGG 25
<210> 615
<211> 25
<212> DNA
<213> Artificial sequence
<400> 615
CATCTGCGTA CAGTTTTCCT GAAAG 25
<210> 616
<211> 24
<212> DNA
<213> Artificial sequence
<400> 616
GAAGACGTGG AAGGTATGGA ATCT 24
<210> 617
<211> 25
<212> DNA
<213> Artificial sequence
<400> 617
CATGAGAAGA TATGTATGGT GGCGA 25
<210> 618
<211> 25
<212> DNA
<213> Artificial sequence
<400> 618
CCAAAAAGCA CAGTATCCTC ATGTT 25
<210> 619
<211> 25
<212> DNA
<213> Artificial sequence
<400> 619
CATGGCTGAA GAGAGTATGG ACATA 25
<210> 620
<211> 25
<212> DNA
<213> Artificial sequence
<400> 620
ATATTTCAGC CCTCCTTCAA TCCTT 25
<210> 621
<211> 25
<212> DNA
<213> Artificial sequence
<400> 621
CATGGTGTGT TGTATTGTAG ACTGG 25
<210> 622
<211> 25
<212> DNA
<213> Artificial sequence
<400> 622
GCTTTGAAAA TGGATGCTTC ACAAG 25
<210> 623
<211> 25
<212> DNA
<213> Artificial sequence
<400> 623
CCACAAAACG GTAATGAACG TATGT 25
<210> 624
<211> 25
<212> DNA
<213> Artificial sequence
<400> 624
ATTGAATGCT TATCAGAGCG TAACC 25
<210> 625
<211> 25
<212> DNA
<213> Artificial sequence
<400> 625
CCAGTCAATC GGTCGATATT GAGTA 25
<210> 626
<211> 25
<212> DNA
<213> Artificial sequence
<400> 626
AGGTCAGGTT ACATAGAAAG GTTCC 25
<210> 627
<211> 25
<212> DNA
<213> Artificial sequence
<400> 627
CCCTTCAGAT GCAAAATACT GGAAT 25
<210> 628
<211> 25
<212> DNA
<213> Artificial sequence
<400> 628
GACGAGCGAG TAAGAATTTG CATAG 25
<210> 629
<211> 24
<212> DNA
<213> Artificial sequence
<400> 629
CCGGGCCTAT AGAGTTGACT TTTT 24
<210> 630
<211> 23
<212> DNA
<213> Artificial sequence
<400> 630
GGAGCAATTC GTAGTAAGGA GCC 23
<210> 631
<211> 25
<212> DNA
<213> Artificial sequence
<400> 631
CCGGTTTCAT TTTGTTCTAC GGTAC 25
<210> 632
<211> 25
<212> DNA
<213> Artificial sequence
<400> 632
GACCGCTACC ATCCTATCAA AATTG 25
<210> 633
<211> 25
<212> DNA
<213> Artificial sequence
<400> 633
CCTCCCGGAA TAGTATTCTA TGCAT 25
<210> 634
<211> 25
<212> DNA
<213> Artificial sequence
<400> 634
GAGGTTTCGT TGTATGCAAA CTAGA 25
<210> 635
<211> 25
<212> DNA
<213> Artificial sequence
<400> 635
CCTGTCCCCT TTTTATCGAA CAAAG 25
<210> 636
<211> 25
<212> DNA
<213> Artificial sequence
<400> 636
ATTGATGGAG TTCGGAAGGA AAGAA 25
<210> 637
<211> 25
<212> DNA
<213> Artificial sequence
<400> 637
CCTTCACTAA TCCAGTTCCG GTTTA 25
<210> 638
<211> 25
<212> DNA
<213> Artificial sequence
<400> 638
GACGATGTTG ATGCGAATTC AAAAC 25
<210> 639
<211> 25
<212> DNA
<213> Artificial sequence
<400> 639
CCTTCCCGTC TACGATTGAA ATTTT 25
<210> 640
<211> 25
<212> DNA
<213> Artificial sequence
<400> 640
TAGAATTAAG GTTGTTGAGC ATGCC 25
<210> 641
<211> 25
<212> DNA
<213> Artificial sequence
<400> 641
CGAACGCAAC GATCATCAGT TATTT 25
<210> 642
<211> 25
<212> DNA
<213> Artificial sequence
<400> 642
TTGTTCATCA CACGAGAAAT GCAAT 25
<210> 643
<211> 25
<212> DNA
<213> Artificial sequence
<400> 643
CGAATGCATT TCTGAACACA GAGAT 25
<210> 644
<211> 25
<212> DNA
<213> Artificial sequence
<400> 644
AACTCATTCA AGTTTAGCAC TCGTG 25
<210> 645
<211> 25
<212> DNA
<213> Artificial sequence
<400> 645
CGACCATGTC AACAAACAAA ACAAA 25
<210> 646
<211> 25
<212> DNA
<213> Artificial sequence
<400> 646
GACAGCAAAG GTGTTTATGG AAAGA 25
<210> 647
<211> 25
<212> DNA
<213> Artificial sequence
<400> 647
CGACCGGAAG ATACCATACC ATTTA 25
<210> 648
<211> 25
<212> DNA
<213> Artificial sequence
<400> 648
TTGTATCCCA AAAGATACCC TGGAG 25
<210> 649
<211> 25
<212> DNA
<213> Artificial sequence
<400> 649
CGACTTTGCC GATGACAGAT TTATT 25
<210> 650
<211> 25
<212> DNA
<213> Artificial sequence
<400> 650
GAGATTTTGG TCCTCCAGAT ACAGT 25
<210> 651
<211> 25
<212> DNA
<213> Artificial sequence
<400> 651
CGAGAAAGAC ATGGAAGTTC AACTG 25
<210> 652
<211> 25
<212> DNA
<213> Artificial sequence
<400> 652
TCCATATGAA GTTCCAATAC AGCCT 25
<210> 653
<211> 25
<212> DNA
<213> Artificial sequence
<400> 653
CGAGACACTG TATAATTGTT GTGGG 25
<210> 654
<211> 23
<212> DNA
<213> Artificial sequence
<400> 654
TTATCGTCGT ACATGGTGGA GTC 23
<210> 655
<211> 25
<212> DNA
<213> Artificial sequence
<400> 655
CGGGAGATTC TGTTGAAGTA TTTGG 25
<210> 656
<211> 25
<212> DNA
<213> Artificial sequence
<400> 656
TTCAAGTACG TTTCTGGAAA AAGGG 25
<210> 657
<211> 25
<212> DNA
<213> Artificial sequence
<400> 657
CGGGTGAGTG AAGAAATGAA ATGAA 25
<210> 658
<211> 25
<212> DNA
<213> Artificial sequence
<400> 658
GTGGATGCCA TGGAGAGAAT TTATG 25
<210> 659
<211> 25
<212> DNA
<213> Artificial sequence
<400> 659
CGTAACAGCT CTATCTTTGA ACGTC 25
<210> 660
<211> 25
<212> DNA
<213> Artificial sequence
<400> 660
CCTTGGTATT CCCGTTCGAC ATATA 25
<210> 661
<211> 25
<212> DNA
<213> Artificial sequence
<400> 661
CGTAATGAAA CGAGAATCGG TAAGG 25
<210> 662
<211> 25
<212> DNA
<213> Artificial sequence
<400> 662
AGCAAATACG GATCCATATC GTACA 25
<210> 663
<211> 26
<212> DNA
<213> Artificial sequence
<400> 663
CGTCAACGTT ATCGACTTTA CTATGG 26
<210> 664
<211> 24
<212> DNA
<213> Artificial sequence
<400> 664
GATAGCACGG TAGTATTGGG GATG 24
<210> 665
<211> 24
<212> DNA
<213> Artificial sequence
<400> 665
CTAGCAAGAA GTCTCAGGGT AGAG 24
<210> 666
<211> 25
<212> DNA
<213> Artificial sequence
<400> 666
ATAACGAGAT TTCATCGAAA GCTGC 25
<210> 667
<211> 25
<212> DNA
<213> Artificial sequence
<400> 667
CTATCTCCAA TCGGTCGCAT TATTC 25
<210> 668
<211> 25
<212> DNA
<213> Artificial sequence
<400> 668
AAGAAGGACC ATCTTCAGGT ACAAT 25
<210> 669
<211> 25
<212> DNA
<213> Artificial sequence
<400> 669
CTATTCGCTT TCCATTCAAT GAGCT 25
<210> 670
<211> 25
<212> DNA
<213> Artificial sequence
<400> 670
GTGCATGTTC GTTATCATAC CAGTC 25
<210> 671
<211> 25
<212> DNA
<213> Artificial sequence
<400> 671
CTCACAATCA AGAAGTCGAA GAGCA 25
<210> 672
<211> 25
<212> DNA
<213> Artificial sequence
<400> 672
ACTACAGCTT GCCCATACTT ATTCT 25
<210> 673
<211> 23
<212> DNA
<213> Artificial sequence
<400> 673
CTCACTGATC TCGAGGCTTT ACG 23
<210> 674
<211> 25
<212> DNA
<213> Artificial sequence
<400> 674
GGATTGAGTA GACGGAGATA TGGAC 25
<210> 675
<211> 25
<212> DNA
<213> Artificial sequence
<400> 675
CTCCTATTCA TTGCTTCAAG TGTGG 25
<210> 676
<211> 24
<212> DNA
<213> Artificial sequence
<400> 676
GAAAGTACAT GGCCTTCATT CTGG 24
<210> 677
<211> 22
<212> DNA
<213> Artificial sequence
<400> 677
CTCTGTATCG AACCTGAGAC CC 22
<210> 678
<211> 25
<212> DNA
<213> Artificial sequence
<400> 678
ACCTCCTAGC TCATCTCGTA AATTC 25
<210> 679
<211> 24
<212> DNA
<213> Artificial sequence
<400> 679
CTCTGTCTGT TCATCGACTC CAAG 24
<210> 680
<211> 25
<212> DNA
<213> Artificial sequence
<400> 680
TTATCCCAAC CCTCAACTCT TCTTC 25
<210> 681
<211> 25
<212> DNA
<213> Artificial sequence
<400> 681
CTCTTAACGA GGAGGAAGCA TCTAT 25
<210> 682
<211> 25
<212> DNA
<213> Artificial sequence
<400> 682
AACTGCAGAG TGATTTCTTT GATGG 25
<210> 683
<211> 25
<212> DNA
<213> Artificial sequence
<400> 683
CTGAACATTG TCGATCGATT GATGT 25
<210> 684
<211> 25
<212> DNA
<213> Artificial sequence
<400> 684
CGGTTATCAA TTGTACTCTT CCTCG 25
<210> 685
<211> 21
<212> DNA
<213> Artificial sequence
<400> 685
CTGAATCTGC GCTCCATCAA C 21
<210> 686
<211> 20
<212> DNA
<213> Artificial sequence
<400> 686
TCACAGCGTC GATCACCTTG 20
<210> 687
<211> 24
<212> DNA
<213> Artificial sequence
<400> 687
CTGCATGGGA GAGTTCAGTA ACAG 24
<210> 688
<211> 25
<212> DNA
<213> Artificial sequence
<400> 688
ATAGAGCTAC ATTTTACCTC GCACT 25
<210> 689
<211> 23
<212> DNA
<213> Artificial sequence
<400> 689
CTGGAGGTGA TGGTCAGAAA ACG 23
<210> 690
<211> 25
<212> DNA
<213> Artificial sequence
<400> 690
AGATGATATT CATACCGACT ACGCG 25
<210> 691
<211> 25
<212> DNA
<213> Artificial sequence
<400> 691
CTTCAGATGT TGGAGCGATG CAATC 25
<210> 692
<211> 21
<212> DNA
<213> Artificial sequence
<400> 692
GTAGGTTTCC GCGGACGTTA G 21
<210> 693
<211> 25
<212> DNA
<213> Artificial sequence
<400> 693
CTTCATCATC CCGAAATGTT GGAAA 25
<210> 694
<211> 25
<212> DNA
<213> Artificial sequence
<400> 694
AAGAATCCTC TAGTTTTGGG CCTTT 25
<210> 695
<211> 25
<212> DNA
<213> Artificial sequence
<400> 695
CTTCGAACAA GGCTACGCAA GATAC 25
<210> 696
<211> 25
<212> DNA
<213> Artificial sequence
<400> 696
CACTTTTGTA TTTTCCGCTT TCTGC 25
<210> 697
<211> 24
<212> DNA
<213> Artificial sequence
<400> 697
CTTGCTCTGG ATCTTCTCAC TCTC 24
<210> 698
<211> 25
<212> DNA
<213> Artificial sequence
<400> 698
TGTACAAGAA AGTTCAAAGA CGCAA 25
<210> 699
<211> 25
<212> DNA
<213> Artificial sequence
<400> 699
CTTTCATTCT TCAATTGGCA TGTCC 25
<210> 700
<211> 25
<212> DNA
<213> Artificial sequence
<400> 700
GGAATCTACG ACCTCGAAAT CTGTA 25
<210> 701
<211> 25
<212> DNA
<213> Artificial sequence
<400> 701
CTTTCTGATG TTACGGTCCC TCTAT 25
<210> 702
<211> 25
<212> DNA
<213> Artificial sequence
<400> 702
TAATAAATGG AGATAAGCGC CGAGA 25
<210> 703
<211> 25
<212> DNA
<213> Artificial sequence
<400> 703
CTTTGGCTTA CATTTCTACC AGTCC 25
<210> 704
<211> 25
<212> DNA
<213> Artificial sequence
<400> 704
CCTTACCCAT ATAGCTGGTC AACTT 25
<210> 705
<211> 25
<212> DNA
<213> Artificial sequence
<400> 705
CTTTTGGTTG TCATAGGCTG GATGT 25
<210> 706
<211> 25
<212> DNA
<213> Artificial sequence
<400> 706
CAGCTCTCGT AAAAACCACA ACTAA 25
<210> 707
<211> 25
<212> DNA
<213> Artificial sequence
<400> 707
GAAAAGCGTT ATACCTATCG ACAGC 25
<210> 708
<211> 25
<212> DNA
<213> Artificial sequence
<400> 708
CAGTGATTTT CGAGACTATG CTGTG 25
<210> 709
<211> 25
<212> DNA
<213> Artificial sequence
<400> 709
GAAAATCAAA CCCGTCGGTA TTCAT 25
<210> 710
<211> 25
<212> DNA
<213> Artificial sequence
<400> 710
GTGAATTGGA GGACATCATT CAAGG 25
<210> 711
<211> 24
<212> DNA
<213> Artificial sequence
<400> 711
GAAAGAAATG ATACGAGCCA GCAC 24
<210> 712
<211> 25
<212> DNA
<213> Artificial sequence
<400> 712
AGATAAGAGG AACCGAACGT GTTTA 25
<210> 713
<211> 25
<212> DNA
<213> Artificial sequence
<400> 713
GAAAGAGCAA ACATGAGATA CGGAG 25
<210> 714
<211> 25
<212> DNA
<213> Artificial sequence
<400> 714
TCCGAGTGAT CAACATTCAC CTAAA 25
<210> 715
<211> 25
<212> DNA
<213> Artificial sequence
<400> 715
GAAAGTTGTC TGTTCTGTCT TCTGG 25
<210> 716
<211> 25
<212> DNA
<213> Artificial sequence
<400> 716
ACGATATTCC TTCACGACTC TTTGA 25
<210> 717
<211> 24
<212> DNA
<213> Artificial sequence
<400> 717
GAAATACCGA TGCTCGCAAA GTAC 24
<210> 718
<211> 25
<212> DNA
<213> Artificial sequence
<400> 718
ACAGTTTCGC CACTAAATCT GATTC 25
<210> 719
<211> 25
<212> DNA
<213> Artificial sequence
<400> 719
GAACAGGCTG AAGTAGATTG AAAGG 25
<210> 720
<211> 25
<212> DNA
<213> Artificial sequence
<400> 720
TATAAGGTGC ATGATGACCT TACCT 25
<210> 721
<211> 25
<212> DNA
<213> Artificial sequence
<400> 721
GAACGATCTG AATGAAGCAT CGATT 25
<210> 722
<211> 23
<212> DNA
<213> Artificial sequence
<400> 722
GAAATAGTCC AACCGCCTCC AAT 23
<210> 723
<211> 25
<212> DNA
<213> Artificial sequence
<400> 723
GAAGAATGAG GGTACATTTC GAAGC 25
<210> 724
<211> 25
<212> DNA
<213> Artificial sequence
<400> 724
GGTAAGAGCA AGATGAAACT ATGGC 25
<210> 725
<211> 24
<212> DNA
<213> Artificial sequence
<400> 725
GAAGAGATGA GCATGGAGAT GAGG 24
<210> 726
<211> 24
<212> DNA
<213> Artificial sequence
<400> 726
GATGTCGTCA AAAGAGCTAA GCAG 24
<210> 727
<211> 25
<212> DNA
<213> Artificial sequence
<400> 727
GAAGCCTGCG AAATACTTGT AGAAG 25
<210> 728
<211> 25
<212> DNA
<213> Artificial sequence
<400> 728
AACTGTAGAA GGAGTCAAAC AGTGA 25
<210> 729
<211> 23
<212> DNA
<213> Artificial sequence
<400> 729
GAAGGATCCT GCACTAGATC GAG 23
<210> 730
<211> 25
<212> DNA
<213> Artificial sequence
<400> 730
TCGATGTATA ATTTGCTTGA CACGG 25
<210> 731
<211> 25
<212> DNA
<213> Artificial sequence
<400> 731
GAATGAAATA GACCAAGACA GAGCA 25
<210> 732
<211> 25
<212> DNA
<213> Artificial sequence
<400> 732
GTGAGTCTCG AGATCCTACA AATCT 25
<210> 733
<211> 23
<212> DNA
<213> Artificial sequence
<400> 733
GACACCATTG ACATAGTCCC CTG 23
<210> 734
<211> 25
<212> DNA
<213> Artificial sequence
<400> 734
AAGGAGTTTA GATTGGATAC GAGGG 25
<210> 735
<211> 25
<212> DNA
<213> Artificial sequence
<400> 735
GACACGTCGA ATCCATCTGT ATTTG 25
<210> 736
<211> 25
<212> DNA
<213> Artificial sequence
<400> 736
AACATTCAGT GATGTTCAAG TCGTC 25
<210> 737
<211> 21
<212> DNA
<213> Artificial sequence
<400> 737
GACCGCAGAG GATGAAGGAA C 21
<210> 738
<211> 25
<212> DNA
<213> Artificial sequence
<400> 738
GGAACCAGCG GATATTAGGT CTATG 25
<210> 739
<211> 23
<212> DNA
<213> Artificial sequence
<400> 739
GACGATCGAG CTGATACCAG TTT 23
<210> 740
<211> 25
<212> DNA
<213> Artificial sequence
<400> 740
ACCTCATCGG AAGTGATTCC AATAT 25
<210> 741
<211> 25
<212> DNA
<213> Artificial sequence
<400> 741
GAGAAGAGTA CCAGTGGAGT TATCC 25
<210> 742
<211> 23
<212> DNA
<213> Artificial sequence
<400> 742
CCCAAAGACA TCCTTCCTGA CTT 23
<210> 743
<211> 25
<212> DNA
<213> Artificial sequence
<400> 743
GAGCAATGAT AGTCTTGATG AGGGA 25
<210> 744
<211> 25
<212> DNA
<213> Artificial sequence
<400> 744
AGCAGGAGAC CCAATCTTTG ATAAT 25
<210> 745
<211> 23
<212> DNA
<213> Artificial sequence
<400> 745
GAGCATTTAA CCCAACCTCA GGT 23
<210> 746
<211> 25
<212> DNA
<213> Artificial sequence
<400> 746
ATATAACCCT GACATCCCAG ACATG 25
<210> 747
<211> 23
<212> DNA
<213> Artificial sequence
<400> 747
GAGGATTGGT GGGTTTTCGA GTC 23
<210> 748
<211> 21
<212> DNA
<213> Artificial sequence
<400> 748
CGAACGGTGC AAGACCAAAA A 21
<210> 749
<211> 25
<212> DNA
<213> Artificial sequence
<400> 749
GAGTTGAGGT GAAATAGCTA CGTTG 25
<210> 750
<211> 25
<212> DNA
<213> Artificial sequence
<400> 750
GGAAAGAGAG TAGCTAATCT TGGGT 25
<210> 751
<211> 24
<212> DNA
<213> Artificial sequence
<400> 751
GATAAATAGC GCTCAGGAAT TGGC 24
<210> 752
<211> 25
<212> DNA
<213> Artificial sequence
<400> 752
TCTGAATTGG GTGGAAATGT TTGAG 25
<210> 753
<211> 25
<212> DNA
<213> Artificial sequence
<400> 753
GATACTATTG GCGAGGATCA GACAG 25
<210> 754
<211> 25
<212> DNA
<213> Artificial sequence
<400> 754
CTTCACATCA CCTGCAACCT ATTAC 25
<210> 755
<211> 25
<212> DNA
<213> Artificial sequence
<400> 755
GATATCACAG TGGAAATGAA CGTCC 25
<210> 756
<211> 25
<212> DNA
<213> Artificial sequence
<400> 756
CGGAAGCTTA AGTTACAGGA ACATG 25
<210> 757
<211> 23
<212> DNA
<213> Artificial sequence
<400> 757
GATATTCTCC GGAAGTGGAA GCC 23
<210> 758
<211> 25
<212> DNA
<213> Artificial sequence
<400> 758
GATCGAAATT GCAACGTGGA ATAAA 25
<210> 759
<211> 25
<212> DNA
<213> Artificial sequence
<400> 759
GATCCTGTCT TGCTCAAACC TAAAC 25
<210> 760
<211> 25
<212> DNA
<213> Artificial sequence
<400> 760
CCGAGTGTAT ATGGCATGTC TCTTA 25
<210> 761
<211> 23
<212> DNA
<213> Artificial sequence
<400> 761
GATGAAGACG AGTTGGATGC TCT 23
<210> 762
<211> 25
<212> DNA
<213> Artificial sequence
<400> 762
GTACTACATC GATGCTACAG GAACT 25
<210> 763
<211> 23
<212> DNA
<213> Artificial sequence
<400> 763
GATGATCCAG CAGGGTTACC AAG 23
<210> 764
<211> 25
<212> DNA
<213> Artificial sequence
<400> 764
CATAGTCTCT ACATCTAACC CAGGC 25
<210> 765
<211> 25
<212> DNA
<213> Artificial sequence
<400> 765
GATGCTGATG TCTGTATCAT TGGTG 25
<210> 766
<211> 25
<212> DNA
<213> Artificial sequence
<400> 766
ACGCCCTGTT CTCTACATTA GTATC 25
<210> 767
<211> 25
<212> DNA
<213> Artificial sequence
<400> 767
GATTCCAGCA TACATCGGTT TTGAA 25
<210> 768
<211> 25
<212> DNA
<213> Artificial sequence
<400> 768
GTATATAGGC ACTGATGGGA GATGG 25
<210> 769
<211> 23
<212> DNA
<213> Artificial sequence
<400> 769
GATTGTTGTT GGGTATACCT GCC 23
<210> 770
<211> 21
<212> DNA
<213> Artificial sequence
<400> 770
TTTCAACCCG ATTGCAAAGC A 21
<210> 771
<211> 25
<212> DNA
<213> Artificial sequence
<400> 771
GCAAGCAATT GAAGGATATC ATCGA 25
<210> 772
<211> 25
<212> DNA
<213> Artificial sequence
<400> 772
GACCAATGAG GAATTTATGC GGTAG 25
<210> 773
<211> 25
<212> DNA
<213> Artificial sequence
<400> 773
GCACCATTGA GTTTGGTACA GAATT 25
<210> 774
<211> 25
<212> DNA
<213> Artificial sequence
<400> 774
GTGGGATAAC TGGAATGGAT GGTAT 25
<210> 775
<211> 25
<212> DNA
<213> Artificial sequence
<400> 775
GCATCATCTT TCCACTTGTT GGTTA 25
<210> 776
<211> 25
<212> DNA
<213> Artificial sequence
<400> 776
TATGGGCTAC ATTATCAAAG CTCCC 25
<210> 777
<211> 25
<212> DNA
<213> Artificial sequence
<400> 777
GCATGAAGCA AAATCTTCGC AAATT 25
<210> 778
<211> 25
<212> DNA
<213> Artificial sequence
<400> 778
AACAAGACCA AGAGCAAATT CTTCG 25
<210> 779
<211> 25
<212> DNA
<213> Artificial sequence
<400> 779
GCCCATTGAA CTTGTTCATC CATAG 25
<210> 780
<211> 25
<212> DNA
<213> Artificial sequence
<400> 780
TTCCATTGCA GACATTAACT ATCGC 25
<210> 781
<211> 25
<212> DNA
<213> Artificial sequence
<400> 781
GCCGATCAAG AGGAAAATCT TTTGA 25
<210> 782
<211> 23
<212> DNA
<213> Artificial sequence
<400> 782
CACTTTTTCT ATGGCGCCGA TTG 23
<210> 783
<211> 23
<212> DNA
<213> Artificial sequence
<400> 783
GCCGCGAATG TACAAATCCA TTT 23
<210> 784
<211> 25
<212> DNA
<213> Artificial sequence
<400> 784
AGTAACGAGG ATGTTATGAA CGACA 25
<210> 785
<211> 25
<212> DNA
<213> Artificial sequence
<400> 785
GCGGTTCATG ATATCATCAT CATCT 25
<210> 786
<211> 25
<212> DNA
<213> Artificial sequence
<400> 786
ATGTTTGAAC AGTTGAAGCT CAGTC 25
<210> 787
<211> 25
<212> DNA
<213> Artificial sequence
<400> 787
GCTAAACCGA TGCTCTTGTA CAAAA 25
<210> 788
<211> 25
<212> DNA
<213> Artificial sequence
<400> 788
TTCTCATACC GAACAACCCA GAAAA 25
<210> 789
<211> 25
<212> DNA
<213> Artificial sequence
<400> 789
GCTGCTGGAC CTTCAAGATA AGTAT 25
<210> 790
<211> 25
<212> DNA
<213> Artificial sequence
<400> 790
CTCACTACAC CCATCTGATA CTTCC 25
<210> 791
<211> 25
<212> DNA
<213> Artificial sequence
<400> 791
GCTTCAACTT TCAAGGTAGC AATCT 25
<210> 792
<211> 25
<212> DNA
<213> Artificial sequence
<400> 792
TCCGTTGATT ATATATAGCC TCTGC 25
<210> 793
<211> 26
<212> DNA
<213> Artificial sequence
<400> 793
GGAAACACAA TTTACACAGT GCATAC 26
<210> 794
<211> 25
<212> DNA
<213> Artificial sequence
<400> 794
TGAGCAATGT ATTCAAAAGT GCTCC 25
<210> 795
<211> 25
<212> DNA
<213> Artificial sequence
<400> 795
GGAATGGAGA GGATCTTCTC GAAAT 25
<210> 796
<211> 25
<212> DNA
<213> Artificial sequence
<400> 796
TCCACAGAAA TTGGCTTGCT ATAAC 25
<210> 797
<211> 25
<212> DNA
<213> Artificial sequence
<400> 797
GGACGCAACG TGGAAGATAT AGATA 25
<210> 798
<211> 25
<212> DNA
<213> Artificial sequence
<400> 798
GCTCAAAATA GATCAGATCG GTTCG 25
<210> 799
<211> 25
<212> DNA
<213> Artificial sequence
<400> 799
GGATAATAAG AGGGACCGTA AACGA 25
<210> 800
<211> 25
<212> DNA
<213> Artificial sequence
<400> 800
AGATTTTCAT CGTCGTCAGA AGTTG 25
<210> 801
<211> 25
<212> DNA
<213> Artificial sequence
<400> 801
GGATATCCAC TATGTCAAAC GAACG 25
<210> 802
<211> 22
<212> DNA
<213> Artificial sequence
<400> 802
CTCACCGAGC AGAGCAAAGA AT 22
<210> 803
<211> 25
<212> DNA
<213> Artificial sequence
<400> 803
GGATTTATGA CGAGCTCGAT TTCTC 25
<210> 804
<211> 23
<212> DNA
<213> Artificial sequence
<400> 804
TGTCTATCCC TACTCTGACT GCG 23
<210> 805
<211> 25
<212> DNA
<213> Artificial sequence
<400> 805
GGGATATATA CCTGTCGCTG ATTCA 25
<210> 806
<211> 25
<212> DNA
<213> Artificial sequence
<400> 806
ATAAACTTAG CTCTCCTACG CTACC 25
<210> 807
<211> 25
<212> DNA
<213> Artificial sequence
<400> 807
GGGGGCCTAC ATCTTATTTG AGATT 25
<210> 808
<211> 25
<212> DNA
<213> Artificial sequence
<400> 808
ACAGTGGTAG AAATGGGAGT AGTTC 25
<210> 809
<211> 25
<212> DNA
<213> Artificial sequence
<400> 809
GGGTGAACTT TGTTGTTGTT GATCT 25
<210> 810
<211> 25
<212> DNA
<213> Artificial sequence
<400> 810
TCGATTCTGC TTGTTCAAAA AGTGA 25
<210> 811
<211> 23
<212> DNA
<213> Artificial sequence
<400> 811
GGTGGATTCT TCACCTTCCA CAT 23
<210> 812
<211> 25
<212> DNA
<213> Artificial sequence
<400> 812
AGTAGTATAT CCAGAACAAC CAGCG 25
<210> 813
<211> 25
<212> DNA
<213> Artificial sequence
<400> 813
GTAAGTGCAG ACTTTGAATG AGTCG 25
<210> 814
<211> 25
<212> DNA
<213> Artificial sequence
<400> 814
ATGACAACAG TGCAATTTCT GTGAT 25
<210> 815
<211> 25
<212> DNA
<213> Artificial sequence
<400> 815
GTATCTGGAC ACCAAACATC TTGTG 25
<210> 816
<211> 25
<212> DNA
<213> Artificial sequence
<400> 816
GATGGCACCT GAGCTAATCA TTCAA 25
<210> 817
<211> 24
<212> DNA
<213> Artificial sequence
<400> 817
GTCAGAAGGG TTGATGGAGA AGAG 24
<210> 818
<211> 24
<212> DNA
<213> Artificial sequence
<400> 818
GACTTTTTGG GCAAGGTTCT GTAG 24
<210> 819
<211> 25
<212> DNA
<213> Artificial sequence
<400> 819
GTCATAAGTC GACTGTGAAA GTGTG 25
<210> 820
<211> 25
<212> DNA
<213> Artificial sequence
<400> 820
TTCAGAGCAG AGTTATGGAG GTTAC 25
<210> 821
<211> 25
<212> DNA
<213> Artificial sequence
<400> 821
GTCGTAGAAG AAAATCGCTG GTATG 25
<210> 822
<211> 25
<212> DNA
<213> Artificial sequence
<400> 822
AGTGATTGTG ATTGTGAAGG GTTTC 25
<210> 823
<211> 25
<212> DNA
<213> Artificial sequence
<400> 823
GTGCAAATTC GAGAATTGTT CGATG 25
<210> 824
<211> 25
<212> DNA
<213> Artificial sequence
<400> 824
TCATTTCCAC TTTCTTGGTA TCAGC 25
<210> 825
<211> 25
<212> DNA
<213> Artificial sequence
<400> 825
GTGGCACTCG AATCTGTACA TGTTC 25
<210> 826
<211> 25
<212> DNA
<213> Artificial sequence
<400> 826
AAGATCCATA GGAGTGTGAA AATGC 25
<210> 827
<211> 25
<212> DNA
<213> Artificial sequence
<400> 827
GTGTCTTCCT CCGAATCTCA AATTC 25
<210> 828
<211> 23
<212> DNA
<213> Artificial sequence
<400> 828
TATACGTGAA GCCAATCCCA CTC 23
<210> 829
<211> 25
<212> DNA
<213> Artificial sequence
<400> 829
GTGTGTGTGA ATCATTAACC CCATT 25
<210> 830
<211> 25
<212> DNA
<213> Artificial sequence
<400> 830
ATCCTCGATG ACAATGACAT ACCAT 25
<210> 831
<211> 25
<212> DNA
<213> Artificial sequence
<400> 831
GTGTTTCACC TCGAATCAGG AAATT 25
<210> 832
<211> 25
<212> DNA
<213> Artificial sequence
<400> 832
ACAAAGTTCG TAAATCCCAT TTGGT 25
<210> 833
<211> 25
<212> DNA
<213> Artificial sequence
<400> 833
GTTAACAAAA CGAACCCAAT ATGCG 25
<210> 834
<211> 25
<212> DNA
<213> Artificial sequence
<400> 834
GTGTCATACT ATTTCCTCAA TCCGG 25
<210> 835
<211> 25
<212> DNA
<213> Artificial sequence
<400> 835
GTTATGACTG AGCAAGAACA AGGAG 25
<210> 836
<211> 25
<212> DNA
<213> Artificial sequence
<400> 836
GAGGAACACT GAAATATCGA CTCCT 25
<210> 837
<211> 25
<212> DNA
<213> Artificial sequence
<400> 837
GTTATGGGAC TTGTAGATGC TGTTC 25
<210> 838
<211> 25
<212> DNA
<213> Artificial sequence
<400> 838
CTGTCAATCT TCATGATTTG CGAGA 25
<210> 839
<211> 25
<212> DNA
<213> Artificial sequence
<400> 839
GTTCTTGCAG AAACATTCAA AGCTG 25
<210> 840
<211> 25
<212> DNA
<213> Artificial sequence
<400> 840
GGTCCTCTAA TATCCAAGCC AATCT 25
<210> 841
<211> 25
<212> DNA
<213> Artificial sequence
<400> 841
GTTGGATTAT GGGTTTAGCG TTGAT 25
<210> 842
<211> 25
<212> DNA
<213> Artificial sequence
<400> 842
AGTTCATGGA CTGAAAAACG AAACT 25
<210> 843
<211> 25
<212> DNA
<213> Artificial sequence
<400> 843
GTTTATCCCG ACATCAATAA CCACA 25
<210> 844
<211> 22
<212> DNA
<213> Artificial sequence
<400> 844
GTAGCCTCGA TGTTGCGTAA TC 22
<210> 845
<211> 25
<212> DNA
<213> Artificial sequence
<400> 845
TAAACGTAGC AAGGTCCTTA ATGCA 25
<210> 846
<211> 25
<212> DNA
<213> Artificial sequence
<400> 846
AATTCCTTAT CCACAACACC ATTGG 25
<210> 847
<211> 25
<212> DNA
<213> Artificial sequence
<400> 847
TAAATGTGGA TTCCGGGTAC GTATT 25
<210> 848
<211> 25
<212> DNA
<213> Artificial sequence
<400> 848
ATCGTCCGTA GAGAAAGTAG CAAAA 25
<210> 849
<211> 25
<212> DNA
<213> Artificial sequence
<400> 849
TAAGAAACAA TGTCCAGACC CTGAT 25
<210> 850
<211> 25
<212> DNA
<213> Artificial sequence
<400> 850
ACAGATATTC ACTCAGTGCT AGCAT 25
<210> 851
<211> 25
<212> DNA
<213> Artificial sequence
<400> 851
TAAGTATCAG GCTCATTGAA CCCTT 25
<210> 852
<211> 25
<212> DNA
<213> Artificial sequence
<400> 852
ACTCAATTTG AACGCGTTTA CTGAT 25
<210> 853
<211> 25
<212> DNA
<213> Artificial sequence
<400> 853
TAATATCATC ACTGACATTG CTGGC 25
<210> 854
<211> 25
<212> DNA
<213> Artificial sequence
<400> 854
CCAAGTCCAT CAGTTCTATA TGGGT 25
<210> 855
<211> 25
<212> DNA
<213> Artificial sequence
<400> 855
TAATATCTTT GTCAGCATTG GTGGG 25
<210> 856
<211> 25
<212> DNA
<213> Artificial sequence
<400> 856
TCTCGCCTGG TAGTATTACA TCAAG 25
<210> 857
<211> 25
<212> DNA
<213> Artificial sequence
<400> 857
TAATCTCCTC ATTTACGCCT CTCTC 25
<210> 858
<211> 25
<212> DNA
<213> Artificial sequence
<400> 858
CCAACCGGAT CATCATAGTA TCCAC 25
<210> 859
<211> 22
<212> DNA
<213> Artificial sequence
<400> 859
TAATGAGCAT CGTACCGTGT GC 22
<210> 860
<211> 24
<212> DNA
<213> Artificial sequence
<400> 860
CTTGGTGCCA CTAGTAGTGT GTTA 24
<210> 861
<211> 25
<212> DNA
<213> Artificial sequence
<400> 861
TACTGGCATC AAATTTTGCT ATCGC 25
<210> 862
<211> 25
<212> DNA
<213> Artificial sequence
<400> 862
GAAGTTCAAT GCCCAGAACC ACATG 25
<210> 863
<211> 25
<212> DNA
<213> Artificial sequence
<400> 863
TACTGGTATG GGATCAGTAG TAGGT 25
<210> 864
<211> 25
<212> DNA
<213> Artificial sequence
<400> 864
ATGGTTGCAT CTGACTCGTA CTATT 25
<210> 865
<211> 25
<212> DNA
<213> Artificial sequence
<400> 865
TACTTCATCG CAATTCAAGT GATCG 25
<210> 866
<211> 25
<212> DNA
<213> Artificial sequence
<400> 866
TGTGTTCTTT GAGACAAGGT TGATG 25
<210> 867
<211> 25
<212> DNA
<213> Artificial sequence
<400> 867
TAGAGAAACA ATACAGTGGC ATGGA 25
<210> 868
<211> 23
<212> DNA
<213> Artificial sequence
<400> 868
TGGAAAGGCC AAGGGTGATA TTC 23
<210> 869
<211> 24
<212> DNA
<213> Artificial sequence
<400> 869
TAGAGGAGGG AGTTGCGATA GTAG 24
<210> 870
<211> 25
<212> DNA
<213> Artificial sequence
<400> 870
AAGTTACTTT ATGGAGACCT CGAGG 25
<210> 871
<211> 25
<212> DNA
<213> Artificial sequence
<400> 871
TAGATTACCT CTGTTGCACT CTTGG 25
<210> 872
<211> 25
<212> DNA
<213> Artificial sequence
<400> 872
GTTAACGTGT ATGTGATCTC ATCGC 25
<210> 873
<211> 25
<212> DNA
<213> Artificial sequence
<400> 873
TAGCAATTGT GGGGATAGAA GAGAG 25
<210> 874
<211> 25
<212> DNA
<213> Artificial sequence
<400> 874
ATTGTCGTCT ATATTGGGCA TGGTA 25
<210> 875
<211> 25
<212> DNA
<213> Artificial sequence
<400> 875
TAGCATGCAC TTTCTATAAC GAGGA 25
<210> 876
<211> 25
<212> DNA
<213> Artificial sequence
<400> 876
GAATGCAACT TCGTTCATTA CTTGC 25
<210> 877
<211> 25
<212> DNA
<213> Artificial sequence
<400> 877
TAGGTTCACT TTCTTCCACT CGTAC 25
<210> 878
<211> 25
<212> DNA
<213> Artificial sequence
<400> 878
CCAAGTCAGG AACCAGTATT TCATT 25
<210> 879
<211> 25
<212> DNA
<213> Artificial sequence
<400> 879
TATGAGGTAC TGAACATTGC GCTTA 25
<210> 880
<211> 26
<212> DNA
<213> Artificial sequence
<400> 880
AGTCCTTCAG TTACCTTATC ATTGGA 26
<210> 881
<211> 25
<212> DNA
<213> Artificial sequence
<400> 881
TATGGGCTAA GAACTTACAG CAACT 25
<210> 882
<211> 25
<212> DNA
<213> Artificial sequence
<400> 882
TATTGAACGC TCCTACCCAG TGATC 25
<210> 883
<211> 25
<212> DNA
<213> Artificial sequence
<400> 883
TATGTAATCG TGAGTACCCG ATGTC 25
<210> 884
<211> 25
<212> DNA
<213> Artificial sequence
<400> 884
TACACGAGAC AAACTCCTAA CTCAG 25
<210> 885
<211> 25
<212> DNA
<213> Artificial sequence
<400> 885
TATTGAGATT CAGGCGGAAG ATGAT 25
<210> 886
<211> 21
<212> DNA
<213> Artificial sequence
<400> 886
GTCACTTCCG ACACTGTCCA C 21
<210> 887
<211> 24
<212> DNA
<213> Artificial sequence
<400> 887
TCAAAAATAT CTGGCCACTC AGGT 24
<210> 888
<211> 26
<212> DNA
<213> Artificial sequence
<400> 888
TCATCTTTTC CTAATCAATC GAAGCT 26
<210> 889
<211> 25
<212> DNA
<213> Artificial sequence
<400> 889
TCACCATGAG ATTGTACCTT GTGTC 25
<210> 890
<211> 24
<212> DNA
<213> Artificial sequence
<400> 890
CTGGGTGAGT GACTCAATTA CGAA 24
<210> 891
<211> 25
<212> DNA
<213> Artificial sequence
<400> 891
TCATAAACAT TGGCTGTATG CATGT 25
<210> 892
<211> 25
<212> DNA
<213> Artificial sequence
<400> 892
TTTCCAGTCA TTCACTAGCT TGGAT 25
<210> 893
<211> 25
<212> DNA
<213> Artificial sequence
<400> 893
TCATCACCTT CTGGAATACG CATAC 25
<210> 894
<211> 25
<212> DNA
<213> Artificial sequence
<400> 894
ATTGTGGAGG TACGGTAACT ACTTC 25
<210> 895
<211> 25
<212> DNA
<213> Artificial sequence
<400> 895
TCATTCCTAA CACCTCTTCG AGTTT 25
<210> 896
<211> 25
<212> DNA
<213> Artificial sequence
<400> 896
TGTTGTTATG CTAACTCATC CCGTA 25
<210> 897
<211> 25
<212> DNA
<213> Artificial sequence
<400> 897
TCCAAACATC AACATAGTTC AACGA 25
<210> 898
<211> 25
<212> DNA
<213> Artificial sequence
<400> 898
AAGTTCATGA GAGAGCTGGA TCATT 25
<210> 899
<211> 25
<212> DNA
<213> Artificial sequence
<400> 899
TCCAGAGAGA TGGATCAAGA AGGTA 25
<210> 900
<211> 25
<212> DNA
<213> Artificial sequence
<400> 900
TCAACTACTT CTTCTTTCGA CCCTT 25
<210> 901
<211> 25
<212> DNA
<213> Artificial sequence
<400> 901
TCCCAGTATT CCAGAAACGG ATATG 25
<210> 902
<211> 25
<212> DNA
<213> Artificial sequence
<400> 902
GTAACGACAA CAAGGTGTGC ATATG 25
<210> 903
<211> 25
<212> DNA
<213> Artificial sequence
<400> 903
TCGATATCGA TGTCAAATGC GTTTT 25
<210> 904
<211> 25
<212> DNA
<213> Artificial sequence
<400> 904
GGATTCAAGG AGAGGAAATT TGACG 25
<210> 905
<211> 25
<212> DNA
<213> Artificial sequence
<400> 905
TCTAGCTTTG ACCAGTTCAT TCTGA 25
<210> 906
<211> 25
<212> DNA
<213> Artificial sequence
<400> 906
ATACCGCAGA AATTGATGAG CTTCA 25
<210> 907
<211> 25
<212> DNA
<213> Artificial sequence
<400> 907
TCTGCCGAGT CTACTAGCTA AATTC 25
<210> 908
<211> 25
<212> DNA
<213> Artificial sequence
<400> 908
GTTGGTTGAA GGACTGACAG TTATG 25
<210> 909
<211> 24
<212> DNA
<213> Artificial sequence
<400> 909
TCTGGTTAAT GCCGGAGATA ATCC 24
<210> 910
<211> 25
<212> DNA
<213> Artificial sequence
<400> 910
CGATCCAGAA AAATCTAACC CACAG 25
<210> 911
<211> 25
<212> DNA
<213> Artificial sequence
<400> 911
TCTGGCAAAG ACATATCAAA GGGAG 25
<210> 912
<211> 25
<212> DNA
<213> Artificial sequence
<400> 912
CAAGATAGAT TTCTGCTGCA TCTGG 25
<210> 913
<211> 28
<212> DNA
<213> Artificial sequence
<400> 913
TCTTCATAGT TGTTGTATAC GAAAGGAC 28
<210> 914
<211> 21
<212> DNA
<213> Artificial sequence
<400> 914
CTATCTTCCT GTGGCCTTGG C 21
<210> 915
<211> 25
<212> DNA
<213> Artificial sequence
<400> 915
TCTTCCTTTA AAACTCTTTC CTGCG 25
<210> 916
<211> 25
<212> DNA
<213> Artificial sequence
<400> 916
AGACTGTGCG AGTAAAAGAC GTATC 25
<210> 917
<211> 25
<212> DNA
<213> Artificial sequence
<400> 917
TGAATTACAA CCAATTGCAA TCCCA 25
<210> 918
<211> 25
<212> DNA
<213> Artificial sequence
<400> 918
ACTGTATTGA CACTCGTGAA GATCA 25
<210> 919
<211> 24
<212> DNA
<213> Artificial sequence
<400> 919
TGACACCAGT CTGACACTTG TTTT 24
<210> 920
<211> 25
<212> DNA
<213> Artificial sequence
<400> 920
GAGACAACAA CCATCATCTC ATGAC 25
<210> 921
<211> 25
<212> DNA
<213> Artificial sequence
<400> 921
TGACATTGAC ATGTTGAACT TGCTC 25
<210> 922
<211> 25
<212> DNA
<213> Artificial sequence
<400> 922
ATTGAAATGT CGATCATGGA GAACG 25
<210> 923
<211> 25
<212> DNA
<213> Artificial sequence
<400> 923
TGACTGTTCT CATATTGGAG GGTTT 25
<210> 924
<211> 25
<212> DNA
<213> Artificial sequence
<400> 924
ATTCAGCTAT GGTAAATGGA GCTCT 25
<210> 925
<211> 25
<212> DNA
<213> Artificial sequence
<400> 925
TGATAAGCCA CCTTGAATCA GAGAT 25
<210> 926
<211> 25
<212> DNA
<213> Artificial sequence
<400> 926
GACATCTGAA TCTTCCATTG CCAAT 25
<210> 927
<211> 25
<212> DNA
<213> Artificial sequence
<400> 927
TGATTTTTCC CAAACATATG CGTCT 25
<210> 928
<211> 25
<212> DNA
<213> Artificial sequence
<400> 928
TTTGAAGAAG TCGTACAAGA TTGGC 25
<210> 929
<211> 25
<212> DNA
<213> Artificial sequence
<400> 929
TGCTATCAGG AAGTAAGTAC GCAAT 25
<210> 930
<211> 25
<212> DNA
<213> Artificial sequence
<400> 930
AGCGGAACTA CTAGAAAAGT CACAA 25
<210> 931
<211> 25
<212> DNA
<213> Artificial sequence
<400> 931
TGGACAAAGT GAGTTTATAT GGGGT 25
<210> 932
<211> 25
<212> DNA
<213> Artificial sequence
<400> 932
CTCGAGTTCT ACGATCCTCT CAATT 25
<210> 933
<211> 25
<212> DNA
<213> Artificial sequence
<400> 933
TGGATCTTCT ATCAATTATC CGGGG 25
<210> 934
<211> 25
<212> DNA
<213> Artificial sequence
<400> 934
CTACCATGAG AGTCCTTTTC TTTGC 25
<210> 935
<211> 25
<212> DNA
<213> Artificial sequence
<400> 935
TGGCTGATAT GCATGAAGCT AAATG 25
<210> 936
<211> 25
<212> DNA
<213> Artificial sequence
<400> 936
ATTAGAAGGT GCTGCCATGT ATAGT 25
<210> 937
<211> 25
<212> DNA
<213> Artificial sequence
<400> 937
TGTAGCCCAT TTCTTTTTCA GGTTC 25
<210> 938
<211> 25
<212> DNA
<213> Artificial sequence
<400> 938
CTTAGGTATA GCGAGATTGA CAACG 25
<210> 939
<211> 25
<212> DNA
<213> Artificial sequence
<400> 939
TGTCTTTGCC CGTTGTACTA TGTAT 25
<210> 940
<211> 24
<212> DNA
<213> Artificial sequence
<400> 940
GAGCCTCCCA AACTAGGTTA ACAG 24
<210> 941
<211> 25
<212> DNA
<213> Artificial sequence
<400> 941
TGTGCACCAA TCTCTACTTT GATTT 25
<210> 942
<211> 25
<212> DNA
<213> Artificial sequence
<400> 942
ATAAGAGAAT AGAAATCGGG GGTGG 25
<210> 943
<211> 25
<212> DNA
<213> Artificial sequence
<400> 943
TGTTGTCGTA AAAATGTTTG AACGC 25
<210> 944
<211> 25
<212> DNA
<213> Artificial sequence
<400> 944
GGAACTTTGG AACGGACTCT TATTC 25
<210> 945
<211> 25
<212> DNA
<213> Artificial sequence
<400> 945
TTATGCAAGC GGATTGAGTG ATAAC 25
<210> 946
<211> 25
<212> DNA
<213> Artificial sequence
<400> 946
CGTAACAGCA TTTCCAGCTC TAATT 25
<210> 947
<211> 25
<212> DNA
<213> Artificial sequence
<400> 947
TTATTATTTC TCTCGCTTGC AGCAC 25
<210> 948
<211> 25
<212> DNA
<213> Artificial sequence
<400> 948
GGCCCCTTAT CTAATCTGAT GGAAT 25
<210> 949
<211> 25
<212> DNA
<213> Artificial sequence
<400> 949
TTCACTCACA ATGGGTTAAG GAAAC 25
<210> 950
<211> 25
<212> DNA
<213> Artificial sequence
<400> 950
GTAGCACCAT TCCTCGAAAA TCTTG 25
<210> 951
<211> 20
<212> DNA
<213> Artificial sequence
<400> 951
TTCATCGTCG TCTTGAGCGG 20
<210> 952
<211> 25
<212> DNA
<213> Artificial sequence
<400> 952
TTGAAACCAG ACAATCCAGG ATGAG 25
<210> 953
<211> 25
<212> DNA
<213> Artificial sequence
<400> 953
TTCATCTGTA TATCATCGAG CCCAG 25
<210> 954
<211> 25
<212> DNA
<213> Artificial sequence
<400> 954
AGAACCTTTA CTTTGGAATT GACCG 25
<210> 955
<211> 23
<212> DNA
<213> Artificial sequence
<400> 955
TTCATCTTGA GCTGTCGACA GAT 23
<210> 956
<211> 25
<212> DNA
<213> Artificial sequence
<400> 956
GAGGAAATAA CGGTTCAGGT AGGAC 25
<210> 957
<211> 25
<212> DNA
<213> Artificial sequence
<400> 957
TTCCACTCGA GATTCAAGAA TGGAT 25
<210> 958
<211> 25
<212> DNA
<213> Artificial sequence
<400> 958
TACTTATTCC CACTTCGTTC TGACC 25
<210> 959
<211> 25
<212> DNA
<213> Artificial sequence
<400> 959
TTCCCTACGG AGATTGGCAT AATAC 25
<210> 960
<211> 25
<212> DNA
<213> Artificial sequence
<400> 960
TGGCTCACAG AAGAATCTAA CCAAT 25
<210> 961
<211> 25
<212> DNA
<213> Artificial sequence
<400> 961
TTCCGAAACA TGTACAGGTC GTATA 25
<210> 962
<211> 25
<212> DNA
<213> Artificial sequence
<400> 962
TAATTGAACG AGGTGGTGCT ATAGG 25
<210> 963
<211> 25
<212> DNA
<213> Artificial sequence
<400> 963
TTCCGAGTAA ATTCACGGAC AGATA 25
<210> 964
<211> 25
<212> DNA
<213> Artificial sequence
<400> 964
AATGGGAAAA AGTTTTGAGA GTCCC 25
<210> 965
<211> 25
<212> DNA
<213> Artificial sequence
<400> 965
TTCTCACACA TACGTTTCAT TCCTC 25
<210> 966
<211> 25
<212> DNA
<213> Artificial sequence
<400> 966
ATATAGATAT GGTTCTCGAC TCCGC 25
<210> 967
<211> 25
<212> DNA
<213> Artificial sequence
<400> 967
TTCTCTATCA TACAGATCCT CAGCC 25
<210> 968
<211> 19
<212> DNA
<213> Artificial sequence
<400> 968
CTCCCGAAGA GCCTGAAAA 19
<210> 969
<211> 25
<212> DNA
<213> Artificial sequence
<400> 969
TTCTGAAGCA AAGAGAGCTA GTAGG 25
<210> 970
<211> 25
<212> DNA
<213> Artificial sequence
<400> 970
ATCATCAGCA AATGTGTATG GTGTG 25
<210> 971
<211> 25
<212> DNA
<213> Artificial sequence
<400> 971
TTCTTGATTC ATACTTCCTC ACCGT 25
<210> 972
<211> 25
<212> DNA
<213> Artificial sequence
<400> 972
CATCAGGATA GGAAGGGAGA TTGAG 25
<210> 973
<211> 25
<212> DNA
<213> Artificial sequence
<400> 973
TTGACTCTTG AGTTGATGGA TCAGA 25
<210> 974
<211> 25
<212> DNA
<213> Artificial sequence
<400> 974
TAGTCTTGGC TTATAGGAAT GCCTT 25
<210> 975
<211> 25
<212> DNA
<213> Artificial sequence
<400> 975
TTGGAAAGTG TGTAACGGAG GTATT 25
<210> 976
<211> 25
<212> DNA
<213> Artificial sequence
<400> 976
TCAAACTGCC GCAACAGATA TAATC 25
<210> 977
<211> 21
<212> DNA
<213> Artificial sequence
<400> 977
TTGGTTTGGG GGTATGCTGT G 21
<210> 978
<211> 25
<212> DNA
<213> Artificial sequence
<400> 978
TGACCTAGAA TTCATGCTAC CGAAT 25
<210> 979
<211> 25
<212> DNA
<213> Artificial sequence
<400> 979
TTGTTTCCCT TTGACTTGTC TGTAC 25
<210> 980
<211> 25
<212> DNA
<213> Artificial sequence
<400> 980
GTAAGGAGTT AGGCAAATGA AGGAG 25
<210> 981
<211> 25
<212> DNA
<213> Artificial sequence
<400> 981
TTTCAACCCC CAGACTCAGA GTTTA 25
<210> 982
<211> 25
<212> DNA
<213> Artificial sequence
<400> 982
CTCAATGAAT GGCAGACAAC TTCTT 25
<210> 983
<211> 25
<212> DNA
<213> Artificial sequence
<400> 983
TTTCACTTAT CGTTACTGGC GAATG 25
<210> 984
<211> 24
<212> DNA
<213> Artificial sequence
<400> 984
GACTGGGTCT CGTATCAATC CAAT 24
<210> 985
<211> 25
<212> DNA
<213> Artificial sequence
<400> 985
TTTCGAAATA GCATCACCCG TAATG 25
<210> 986
<211> 25
<212> DNA
<213> Artificial sequence
<400> 986
CCGAATCATG CTGCTAATCA TTTCT 25
<210> 987
<211> 25
<212> DNA
<213> Artificial sequence
<400> 987
TTTCTGGCTT TGGATCAGAG AACAA 25
<210> 988
<211> 25
<212> DNA
<213> Artificial sequence
<400> 988
CATCATGAAA ATCTTTCGTC AACGC 25
<210> 989
<211> 25
<212> DNA
<213> Artificial sequence
<400> 989
TTTCTGTAAT GACACCTTAG CTGGA 25
<210> 990
<211> 27
<212> DNA
<213> Artificial sequence
<400> 990
TCAAGTTCCG TTGCTATAAT TGTAACA 27
<210> 991
<211> 25
<212> DNA
<213> Artificial sequence
<400> 991
TTTGACTCCA AGTGTAAATT CACGG 25
<210> 992
<211> 25
<212> DNA
<213> Artificial sequence
<400> 992
GAGTCCCAAA ATTGAAGTCA GTGAT 25
<210> 993
<211> 25
<212> DNA
<213> Artificial sequence
<400> 993
TTTGCTTCTT CGGAGTAACA TGAAC 25
<210> 994
<211> 25
<212> DNA
<213> Artificial sequence
<400> 994
TTCCTGCTGT CAGAGTTTAT GTGTA 25
<210> 995
<211> 25
<212> DNA
<213> Artificial sequence
<400> 995
TTTTGTTGTC GTTCGTTTGT TGTTC 25
<210> 996
<211> 25
<212> DNA
<213> Artificial sequence
<400> 996
GTATCCGATA CATCATGCTA GCAGA 25
<210> 997
<211> 25
<212> DNA
<213> Artificial sequence
<400> 997
CCATATTTAT GTCCTAATCA CGGCG 25
<210> 998
<211> 25
<212> DNA
<213> Artificial sequence
<400> 998
CACAGATAGT GCAGGAGTAA CGTAG 25
<210> 999
<211> 25
<212> DNA
<213> Artificial sequence
<400> 999
CTCTTCTTCT TCTTTCTCCT CCTCC 25
<210> 1000
<211> 25
<212> DNA
<213> Artificial sequence
<400> 1000
AGTGATATAG CTGGGCTGTT TAACA 25
<210> 1001
<211> 25
<212> DNA
<213> Artificial sequence
<400> 1001
GTAATCTGAT GAAAAGGAGC TGGAC 25
<210> 1002
<211> 25
<212> DNA
<213> Artificial sequence
<400> 1002
CTAAAACCAC CAAACCAAAA GCGTT 25
Claims (8)
1.引物组合甲,包括引物1—引物1002;
所述引物1—引物1002的核苷酸序列依次如SEQ ID NO:1-SEQ ID NO:1002所示。
2.根据权利要求1所述的引物组合甲,其特征在于:所述引物组合甲由引物1—引物1002组成。
3.引物组合乙,由引物5、引物6、引物7、引物8、引物11、引物12、引物15、引物16、引物19、引物20、引物21、引物22、引物25、引物26、引物35、引物36、引物45、引物46、引物49、引物50、引物51、引物52、引物67、引物68、引物73、引物74、引物87、引物88、引物97、引物98、引物99、引物100、引物105、引物106、引物115、引物116、引物117、引物118、引物131、引物132、引物139、引物140、引物145、引物146、引物147、引物148、引物165、引物166、引物171、引物172、引物173、引物174、引物177、引物178、引物179、引物180、引物181、引物182、引物183、引物184、引物187、引物188、引物195、引物196、引物197、引物198、引物205、引物206、引物213、引物214、引物215、引物216、引物227、引物228、引物237、引物238、引物239、引物240、引物243、引物244、引物253、引物254、引物255、引物256、引物257、引物258、引物263、引物264、引物265、引物266、引物267、引物268、引物271、引物272、引物273、引物274、引物281、引物282、引物283、引物284、引物287、引物288、引物295、引物296、引物299、引物300、引物301、引物302、引物303、引物304、引物313、引物314、引物315、引物316、引物321、引物322、引物339、引物340、引物347、引物348、引物355、引物356、引物375、引物376、引物379、引物380、引物381、引物382、引物383、引物384、引物393、引物394、引物395、引物396、引物397、引物398、引物399、引物400、引物407、引物408、引物411、引物412、引物423、引物424、引物429、引物430、引物435、引物436、引物437、引物438、引物441、引物442、引物451、引物452、引物463、引物464、引物465、引物466、引物473、引物474、引物475、引物476、引物477、引物478、引物479、引物480、引物491、引物492、引物493、引物494、引物495、引物496、引物497、引物498、引物501、引物502、引物507、引物508、引物511、引物512、引物513、引物514、引物515、引物516、引物517、引物518、引物519、引物520、引物523、引物524、引物529、引物530、引物531、引物532、引物533、引物534、引物535、引物536、引物539、引物540、引物541、引物542、引物549、引物550、引物553、引物554、引物557、引物558、引物559、引物560、引物563、引物564、引物567、引物568、引物571、引物572、引物573、引物574、引物579、引物580、引物583、引物584、引物605、引物606、引物607、引物608、引物611、引物612、引物615、引物616、引物621、引物622、引物623、引物624、引物627、引物628、引物629、引物630、引物635、引物636、引物643、引物644、引物649、引物650、引物661、引物662、引物665、引物666、引物673、引物674、引物685、引物686、引物687、引物688、引物691、引物692、引物693、引物694、引物695、引物696、引物703、引物704、引物705、引物706、引物713、引物714、引物717、引物718、引物723、引物724、引物725、引物726、引物727、引物728、引物729、引物730、引物731、引物732、引物739、引物740、引物741、引物742、引物743、引物744、引物747、引物748、引物749、引物750、引物751、引物752、引物753、引物754、引物755、引物756、引物767、引物768、引物769、引物770、引物775、引物776、引物779、引物780、引物807、引物808、引物813、引物814、引物823、引物824、引物831、引物832、引物833、引物834、引物835、引物836、引物843、引物844、引物845、引物846、引物851、引物852、引物857、引物858、引物861、引物862、引物871、引物872、引物875、引物876、引物885、引物886、引物893、引物894、引物909、引物910、引物911、引物912、引物913、引物914、引物915、引物916、引物923、引物924、引物927、引物928、引物929、引物930、引物933、引物934、引物937、引物938、引物939、引物940、引物947、引物948、引物951、引物952、引物953、引物954、引物959、引物960、引物961、引物962、引物965、引物966、引物967、引物968、引物971、引物972、引物977、引物978、引物989、引物990、引物999和引物1000组成;
引物5、引物6、引物7、引物8、引物11、引物12、引物15、引物16、引物19、引物20、引物21、引物22、引物25、引物26、引物35、引物36、引物45、引物46、引物49、引物50、引物51、引物52、引物67、引物68、引物73、引物74、引物87、引物88、引物97、引物98、引物99、引物100、引物105、引物106、引物115、引物116、引物117、引物118、引物131、引物132、引物139、引物140、引物145、引物146、引物147、引物148、引物165、引物166、引物171、引物172、引物173、引物174、引物177、引物178、引物179、引物180、引物181、引物182、引物183、引物184、引物187、引物188、引物195、引物196、引物197、引物198、引物205、引物206、引物213、引物214、引物215、引物216、引物227、引物228、引物237、引物238、引物239、引物240、引物243、引物244、引物253、引物254、引物255、引物256、引物257、引物258、引物263、引物264、引物265、引物266、引物267、引物268、引物271、引物272、引物273、引物274、引物281、引物282、引物283、引物284、引物287、引物288、引物295、引物296、引物299、引物300、引物301、引物302、引物303、引物304、引物313、引物314、引物315、引物316、引物321、引物322、引物339、引物340、引物347、引物348、引物355、引物356、引物375、引物376、引物379、引物380、引物381、引物382、引物383、引物384、引物393、引物394、引物395、引物396、引物397、引物398、引物399、引物400、引物407、引物408、引物411、引物412、引物423、引物424、引物429、引物430、引物435、引物436、引物437、引物438、引物441、引物442、引物451、引物452、引物463、引物464、引物465、引物466、引物473、引物474、引物475、引物476、引物477、引物478、引物479、引物480、引物491、引物492、引物493、引物494、引物495、引物496、引物497、引物498、引物501、引物502、引物507、引物508、引物511、引物512、引物513、引物514、引物515、引物516、引物517、引物518、引物519、引物520、引物523、引物524、引物529、引物530、引物531、引物532、引物533、引物534、引物535、引物536、引物539、引物540、引物541、引物542、引物549、引物550、引物553、引物554、引物557、引物558、引物559、引物560、引物563、引物564、引物567、引物568、引物571、引物572、引物573、引物574、引物579、引物580、引物583、引物584、引物605、引物606、引物607、引物608、引物611、引物612、引物615、引物616、引物621、引物622、引物623、引物624、引物627、引物628、引物629、引物630、引物635、引物636、引物643、引物644、引物649、引物650、引物661、引物662、引物665、引物666、引物673、引物674、引物685、引物686、引物687、引物688、引物691、引物692、引物693、引物694、引物695、引物696、引物703、引物704、引物705、引物706、引物713、引物714、引物717、引物718、引物723、引物724、引物725、引物726、引物727、引物728、引物729、引物730、引物731、引物732、引物739、引物740、引物741、引物742、引物743、引物744、引物747、引物748、引物749、引物750、引物751、引物752、引物753、引物754、引物755、引物756、引物767、引物768、引物769、引物770、引物775、引物776、引物779、引物780、引物807、引物808、引物813、引物814、引物823、引物824、引物831、引物832、引物833、引物834、引物835、引物836、引物843、引物844、引物845、引物846、引物851、引物852、引物857、引物858、引物861、引物862、引物871、引物872、引物875、引物876、引物885、引物886、引物893、引物894、引物909、引物910、引物911、引物912、引物913、引物914、引物915、引物916、引物923、引物924、引物927、引物928、引物929、引物930、引物933、引物934、引物937、引物938、引物939、引物940、引物947、引物948、引物951、引物952、引物953、引物954、引物959、引物960、引物961、引物962、引物965、引物966、引物967、引物968、引物971、引物972、引物977、引物978、引物989、引物990、引物999和引物1000的核苷酸序列依次如SEQ ID NO:5、SEQ ID NO:6、SEQ ID NO:7、SEQ ID NO:8、SEQ ID NO:11、SEQ ID NO:12、SEQ ID NO:15、SEQ ID NO:16、SEQ ID NO:19、SEQ ID NO:20、SEQ ID NO:21、SEQ ID NO:22、SEQ ID NO:25、SEQ ID NO:26、SEQ ID NO:35、SEQ ID NO:36、SEQ IDNO:45、SEQ ID NO:46、SEQ ID NO:49、SEQ ID NO:50、SEQ ID NO:51、SEQ ID NO:52、SEQ IDNO:67、SEQ ID NO:68、SEQ ID NO:73、SEQ ID NO:74、SEQ ID NO:87、SEQ ID NO:88、SEQ IDNO:97、SEQ ID NO:98、SEQ ID NO:99、SEQ ID NO:100、SEQ ID NO:105、SEQ ID NO:106、SEQID NO:115、SEQ ID NO:116、SEQ ID NO:117、SEQ ID NO:118、SEQ ID NO:131、SEQ ID NO:132、SEQ ID NO:139、SEQ ID NO:140、SEQ ID NO:145、SEQ ID NO:146、SEQ ID NO:147、SEQID NO:148、SEQ ID NO:165、SEQ ID NO:166、SEQ ID NO:171、SEQ ID NO:172、SEQ ID NO:173、SEQ ID NO:174、SEQ ID NO:177、SEQ ID NO:178、SEQ ID NO:179、SEQ ID NO:180、SEQID NO:181、SEQ ID NO:182、SEQ ID NO:183、SEQ ID NO:184、SEQ ID NO:187、SEQ ID NO:188、SEQ ID NO:195、SEQ ID NO:196、SEQ ID NO:197、SEQ ID NO:198、SEQ ID NO:205、SEQID NO:206、SEQ ID NO:213、SEQ ID NO:214、SEQ ID NO:215、SEQ ID NO:216、SEQ ID NO:227、SEQ ID NO:228、SEQ ID NO:237、SEQ ID NO:238、SEQ ID NO:239、SEQ ID NO:240、SEQID NO:243、SEQ ID NO:244、SEQ ID NO:253、SEQ ID NO:254、SEQ ID NO:255、SEQ ID NO:256、SEQ ID NO:257、SEQ ID NO:258、SEQ ID NO:263、SEQ ID NO:264、SEQ ID NO:265、SEQID NO:266、SEQ ID NO:267、SEQ ID NO:268、SEQ ID NO:271、SEQ ID NO:272、SEQ ID NO:273、SEQ ID NO:274、SEQ ID NO:281、SEQ ID NO:282、SEQ ID NO:283、SEQ ID NO:284、SEQID NO:287、SEQ ID NO:288、SEQ ID NO:295、SEQ ID NO:296、SEQ ID NO:299、SEQ ID NO:300、SEQ ID NO:301、SEQ ID NO:302、SEQ ID NO:303、SEQ ID NO:304、SEQ ID NO:313、SEQID NO:314、SEQ ID NO:315、SEQ ID NO:316、SEQ ID NO:321、SEQ ID NO:322、SEQ ID NO:339、SEQ ID NO:340、SEQ ID NO:347、SEQ ID NO:348、SEQ ID NO:355、SEQ ID NO:356、SEQID NO:375、SEQ ID NO:376、SEQ ID NO:379、SEQ ID NO:380、SEQ ID NO:381、SEQ ID NO:382、SEQ ID NO:383、SEQ ID NO:384、SEQ ID NO:393、SEQ ID NO:394、SEQ ID NO:395、SEQID NO:396、SEQ ID NO:397、SEQ ID NO:398、SEQ ID NO:399、SEQ ID NO:400、SEQ ID NO:407、SEQ ID NO:408、SEQ ID NO:411、SEQ ID NO:412、SEQ ID NO:423、SEQ ID NO:424、SEQID NO:429、SEQ ID NO:430、SEQ ID NO:435、SEQ ID NO:436、SEQ ID NO:437、SEQ ID NO:438、SEQ ID NO:441、SEQ ID NO:442、SEQ ID NO:451、SEQ ID NO:452、SEQ ID NO:463、SEQID NO:464、SEQ ID NO:465、SEQ ID NO:466、SEQ ID NO:473、SEQ ID NO:474、SEQ ID NO:475、SEQ ID NO:476、SEQ ID NO:477、SEQ ID NO:478、SEQ ID NO:479、SEQ ID NO:480、SEQID NO:491、SEQ ID NO:492、SEQ ID NO:493、SEQ ID NO:494、SEQ ID NO:495、SEQ ID NO:496、SEQ ID NO:497、SEQ ID NO:498、SEQ ID NO:501、SEQ ID NO:502、SEQ ID NO:507、SEQID NO:508、SEQ ID NO:511、SEQ ID NO:512、SEQ ID NO:513、SEQ ID NO:514、SEQ ID NO:515、SEQ ID NO:516、SEQ ID NO:517、SEQ ID NO:518、SEQ ID NO:519、SEQ ID NO:520、SEQID NO:523、SEQ ID NO:524、SEQ ID NO:529、SEQ ID NO:530、SEQ ID NO:531、SEQ ID NO:532、SEQ ID NO:533、SEQ ID NO:534、SEQ ID NO:535、SEQ ID NO:536、SEQ ID NO:539、SEQID NO:540、SEQ ID NO:541、SEQ ID NO:542、SEQ ID NO:549、SEQ ID NO:550、SEQ ID NO:553、SEQ ID NO:554、SEQ ID NO:557、SEQ ID NO:558、SEQ ID NO:559、SEQ ID NO:560、SEQID NO:563、SEQ ID NO:564、SEQ ID NO:567、SEQ ID NO:568、SEQ ID NO:571、SEQ ID NO:572、SEQ ID NO:573、SEQ ID NO:574、SEQ ID NO:579、SEQ ID NO:580、SEQ ID NO:583、SEQID NO:584、SEQ ID NO:605、SEQ ID NO:606、SEQ ID NO:607、SEQ ID NO:608、SEQ ID NO:611、SEQ ID NO:612、SEQ ID NO:615、SEQ ID NO:616、SEQ ID NO:621、SEQ ID NO:622、SEQID NO:623、SEQ ID NO:624、SEQ ID NO:627、SEQ ID NO:628、SEQ ID NO:629、SEQ ID NO:630、SEQ ID NO:635、SEQ ID NO:636、SEQ ID NO:643、SEQ ID NO:644、SEQ ID NO:649、SEQID NO:650、SEQ ID NO:661、SEQ ID NO:662、SEQ ID NO:665、SEQ ID NO:666、SEQ ID NO:673、SEQ ID NO:674、SEQ ID NO:685、SEQ ID NO:686、SEQ ID NO:687、SEQ ID NO:688、SEQID NO:691、SEQ ID NO:692、SEQ ID NO:693、SEQ ID NO:694、SEQ ID NO:695、SEQ ID NO:696、SEQ ID NO:703、SEQ ID NO:704、SEQ ID NO:705、SEQ ID NO:706、SEQ ID NO:713、SEQID NO:714、SEQ ID NO:717、SEQ ID NO:718、SEQ ID NO:723、SEQ ID NO:724、SEQ ID NO:725、SEQ ID NO:726、SEQ ID NO:727、SEQ ID NO:728、SEQ ID NO:729、SEQ ID NO:730、SEQID NO:731、SEQ ID NO:732、SEQ ID NO:739、SEQ ID NO:740、SEQ ID NO:741、SEQ ID NO:742、SEQ ID NO:743、SEQ ID NO:744、SEQ ID NO:747、SEQ ID NO:748、SEQ ID NO:749、SEQID NO:750、SEQ ID NO:751、SEQ ID NO:752、SEQ ID NO:753、SEQ ID NO:754、SEQ ID NO:755、SEQ ID NO:756、SEQ ID NO:767、SEQ ID NO:768、SEQ ID NO:769、SEQ ID NO:770、SEQID NO:775、SEQ ID NO:776、SEQ ID NO:779、SEQ ID NO:780、SEQ ID NO:807、SEQ ID NO:808、SEQ ID NO:813、SEQ ID NO:814、SEQ ID NO:823、SEQ ID NO:824、SEQ ID NO:831、SEQID NO:832、SEQ ID NO:833、SEQ ID NO:834、SEQ ID NO:835、SEQ ID NO:836、SEQ ID NO:843、SEQ ID NO:844、SEQ ID NO:845、SEQ ID NO:846、SEQ ID NO:851、SEQ ID NO:852、SEQID NO:857、SEQ ID NO:858、SEQ ID NO:861、SEQ ID NO:862、SEQ ID NO:871、SEQ ID NO:872、SEQ ID NO:875、SEQ ID NO:876、SEQ ID NO:885、SEQ ID NO:886、SEQ ID NO:893、SEQID NO:894、SEQ ID NO:909、SEQ ID NO:910、SEQ ID NO:911、SEQ ID NO:912、SEQ ID NO:913、SEQ ID NO:914、SEQ ID NO:915、SEQ ID NO:916、SEQ ID NO:923、SEQ ID NO:924、SEQID NO:927、SEQ ID NO:928、SEQ ID NO:929、SEQ ID NO:930、SEQ ID NO:933、SEQ ID NO:934、SEQ ID NO:937、SEQ ID NO:938、SEQ ID NO:939、SEQ ID NO:940、SEQ ID NO:947、SEQID NO:948、SEQ ID NO:951、SEQ ID NO:952、SEQ ID NO:953、SEQ ID NO:954、SEQ ID NO:959、SEQ ID NO:960、SEQ ID NO:961、SEQ ID NO:962、SEQ ID NO:965、SEQ ID NO:966、SEQID NO:967、SEQ ID NO:968、SEQ ID NO:971、SEQ ID NO:972、SEQ ID NO:977、SEQ ID NO:978、SEQ ID NO:989、SEQ ID NO:990、SEQ ID NO:999和SEQ ID NO:1000。
4.权利要求1或2所述引物组合甲或权利要求3所述引物组合乙在香菇品种鉴定中的应用。
5.权利要求1或2所述引物组合甲或权利要求3所述引物组合乙在鉴别香菇品种真伪性中的应用。
6.权利要求1或2所述引物组合甲或权利要求3所述引物组合乙在鉴定待测香菇菌株是否为同一品种或同一谱系中的应用。
7.一种鉴定若干待测香菇菌株是否为同一品种或同一谱系的方法,依次包括如下步骤:
(1)分别以若干待测香菇菌株的基因组DNA为模板,采用权利要求1或2所述引物组合甲或权利要求3所述引物组合乙进行多重PCR扩增,得到PCR扩增产物;
(2)分别将PCR扩增产物进行测序;
(3)分别将PCR扩增产物测序结果与香菇MNP标记文库中进行比对,计算待测香菇菌株和香菇MNP标记文库中菌株间的两两遗传相似度GS,之后进行如下判断:如果遗传相似度GS小于94%,则两个待测香菇菌株为不同品种且不同谱系;如果遗传相似度GS为94%以上且小于99.5%,则两个待测香菇菌株为同一谱系内的不同品种;如果遗传相似度GS为99.5%以上,则两个待测香菇菌株为同一谱系内的同一品种。
8.根据权利要求7所述的方法,其特征在于:所述香菇MNP标记文库的制备方法如下:分别提取具有代表性的香菇菌株的基因组DNA,采用权利要求1或2所述引物组合甲或权利要求3所述引物组合乙进行多重PCR扩增,得到PCR扩增产物;之后测序,所有测序结果组成香菇MNP标记文库。
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