CN117088995A - Highland barley beta-glucan and preparation method thereof - Google Patents

Highland barley beta-glucan and preparation method thereof Download PDF

Info

Publication number
CN117088995A
CN117088995A CN202311170986.2A CN202311170986A CN117088995A CN 117088995 A CN117088995 A CN 117088995A CN 202311170986 A CN202311170986 A CN 202311170986A CN 117088995 A CN117088995 A CN 117088995A
Authority
CN
China
Prior art keywords
highland barley
glucan
barley beta
beta
ethanol
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202311170986.2A
Other languages
Chinese (zh)
Inventor
游琳珊
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tibet Cangguyuan Food Technology Co ltd
Original Assignee
Tibet Cangguyuan Food Technology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tibet Cangguyuan Food Technology Co ltd filed Critical Tibet Cangguyuan Food Technology Co ltd
Priority to CN202311170986.2A priority Critical patent/CN117088995A/en
Publication of CN117088995A publication Critical patent/CN117088995A/en
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08BPOLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0024Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08BPOLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0003General processes for their isolation or fractionation, e.g. purification or extraction from biomass

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Materials Engineering (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Organic Chemistry (AREA)
  • Sustainable Development (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

The application is applicable to the technical field of biological medicine, and provides highland barley beta-glucan and a preparation method thereof, wherein the preparation method comprises the following steps: step one, raw material preparation: peeling highland barley to obtain highland barley rice; step two, grinding highland barley: pulverizing semen Avenae Nudae, and sieving with 40-80 mesh sieve to obtain semen Avenae Nudae powder; step three, alkali liquor extraction: extracting at 30-50deg.C for 2 hr at pH of 7-10; step four, mixing and adjusting: the highland barley powder is added with water with the feed liquid ratio of 1g (5-80) mL, alkali liquid is added to adjust the pH value to 8-10, then 20U-2000U of high-temperature amylase is added, the mixture is uniformly mixed and stirred and refluxed for 1-15h at the temperature of 90-95 ℃, the characteristics of micromolecular polysaccharide are removed by utilizing membrane separation, and meanwhile, the characteristics of fractional alcohol precipitation and dialysis purification treatment are utilized, the purity of the obtained highland barley beta-glucan is high, the process is simple and convenient, the production cost is lower, more ethanol can be obtained for recycling, the water after the ethanol is recycled can be reused, and the whole extraction process has no pollutant discharge, thereby being beneficial to protecting the environment.

Description

Highland barley beta-glucan and preparation method thereof
Technical Field
The application belongs to the technical field of biological medicines, and particularly relates to highland barley beta-glucan and a preparation method thereof.
Background
Highland barley (hordeum vulgar l.var.nudur hook.f) belongs to the genus barley crop of the family gramineae, also known as naked barley, rice barley, huai barley and meta-wheat. The content of beta-glucan in highland barley raw materials is 4% -8%, and researches show that the beta-glucan has the effects of reducing blood fat, reducing cholesterol, regulating blood sugar, preventing cardiovascular diseases, improving immunity, resisting tumors and the like.
The prior art has the following defects:
at present, the preparation method of the high-content highland barley beta-glucan has the processes of alcohol precipitation, deproteinization, depigmentation, preparation chromatography purification and the like, is relatively complex, is relatively difficult to separate, is easy to cause lower yield of the beta-glucan in the extraction process, and has the defect of high water consumption or high treatment cost caused by the consumption of ethanol in the traditional method for extracting the beta-glucan by an alkaline method or an acid method.
Disclosure of Invention
The application provides highland barley beta-glucan and a preparation method thereof, and aims to solve the problems that the existing preparation method of highland barley beta-glucan with high content has the processes of alcohol precipitation, deproteinization, depigmentation, preparation chromatography purification and the like, is complex, is difficult to separate, is easy to cause lower yield of beta-glucan in the extraction process, and has the defects of high water consumption or high ethanol consumption and high treatment cost in the traditional method for extracting beta-glucan by an alkaline method or an acid method.
The application is realized in this way, a highland barley beta-glucan and its preparation method, comprising:
step one, raw material preparation: peeling highland barley to obtain highland barley rice;
step two, grinding highland barley: pulverizing semen Avenae Nudae, and sieving with 40-80 mesh sieve to obtain semen Avenae Nudae powder;
step three, alkali liquor extraction: extracting at 30-50deg.C for 2 hr at pH of 7-10;
step four, mixing and adjusting: adding water with a feed liquid ratio of 1g (5-80) mL into highland barley powder, adding alkali liquid to adjust the pH value to 8-10, then adding 20U-2000U of high-temperature amylase, uniformly mixing, and stirring and refluxing for 1-15h at 90-95 ℃;
fifthly, removing protein: adjusting the pH value of the solution obtained in the step four to 3-5, standing, precipitating protein, centrifuging to remove the precipitate, and preparing an extracting solution;
step six, removing small molecule polysaccharide: intercepting the centrifugate by using an organic or inorganic ultrafiltration membrane with molecular weight of 5-10 ten thousand Da, and collecting the permeate;
step seven, centrifugal extraction: centrifuging for 10 minutes by a 4000-turn centrifuge to remove starch and obtain supernatant;
step eight, concentration treatment: concentrating under reduced pressure;
step nine, grading alcohol precipitation: concentrating the permeate, carrying out fractional alcohol precipitation, collecting precipitate from low to high concentration of alcohol precipitation, and obtaining highland barley beta-glucan crude product;
step ten, filtering: re-suspending the filter residue, stirring again, standing and filtering;
step eleven, dialysis and purification: adding water into the highland barley beta-glucan crude product for re-dissolution, and then dialyzing and purifying by using 8-15 ten thousand cellulose dialysis bags, and collecting highland barley beta-glucan pure product solution;
step twelve, drying: drying the highland barley beta-glucan pure product solution to obtain highland barley beta-glucan pure product;
step thirteen, ethanol recovery: and recycling ethanol by adopting an ethanol recycling tower.
Preferably, the drying temperature in the step twelve is 40-60 ℃ and the drying time is 4 hours.
Preferably, the conditions of the alcohol precipitation process in the step nine are as follows: the ethanol concentration is 95%, the edible ethanol with the mass 1-2 times of that of the concentrated solution is added, and the mixture is stirred for 10min and then is kept stand for 30min, and the mixture is filtered after standing.
Preferably, in the step seven, the centrifugation mode utilizes a decanter centrifuge and a disk centrifuge to centrifuge the extracting solution respectively.
Preferably, the pressure of the pressure concentration in the step eight is-0.06 to-0.08 MPa, and the temperature is 60 ℃.
Preferably, the highland barley beta-glucan is prepared by the preparation method of the highland barley beta-glucan of claims 1-5.
Compared with the prior art, the embodiment of the application has the following main beneficial effects:
the method has the advantages that the characteristics of small molecular polysaccharide are removed by utilizing membrane separation, and meanwhile, the grading alcohol precipitation and dialysis purification treatment are utilized, so that the obtained highland barley beta-glucan has high purity, the process is simple and convenient, the production cost is low, more ethanol can be recycled, water after the ethanol is recovered can be reused, no pollutant is discharged in the whole extraction process, and the environment is favorably protected.
Drawings
FIG. 1 is a schematic flow chart of a preparation method of highland barley beta-glucan;
Detailed Description
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the terminology used in the description of the applications herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application; the terms "comprising" and "having" and any variations thereof in the description of the application and the claims and the description of the drawings above are intended to cover a non-exclusive inclusion. The terms first, second and the like in the description and in the claims or in the above-described figures, are used for distinguishing between different objects and not necessarily for describing a sequential or chronological order.
Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the application. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those of skill in the art will explicitly and implicitly appreciate that the embodiments described herein may be combined with other embodiments.
The embodiment of the application provides highland barley beta-glucan and a preparation method thereof, as shown in figure 1, comprising the following steps:
step one, raw material preparation: peeling highland barley to obtain highland barley rice;
step two, grinding highland barley: pulverizing semen Avenae Nudae, and sieving with 40-80 mesh sieve to obtain semen Avenae Nudae powder;
step three, alkali liquor extraction: extracting at 30-50deg.C for 2 hr at pH of 7-10;
step four, mixing and adjusting: adding water with a feed liquid ratio of 1g (5-80) mL into highland barley powder, adding alkali liquid to adjust the pH value to 8-10, then adding 20U-2000U of high-temperature amylase, uniformly mixing, and stirring and refluxing for 1-15h at 90-95 ℃;
fifthly, removing protein: adjusting the pH value of the solution obtained in the step four to 3-5, standing, precipitating protein, centrifuging to remove the precipitate, and preparing an extracting solution;
step six, removing small molecule polysaccharide: intercepting the centrifugate by using an organic or inorganic ultrafiltration membrane with molecular weight of 5-10 ten thousand Da, and collecting the permeate;
step seven, centrifugal extraction: centrifuging for 10 minutes by a 4000-turn centrifuge to remove starch and obtain supernatant;
step eight, concentration treatment: concentrating under reduced pressure;
step nine, grading alcohol precipitation: concentrating the permeate, carrying out fractional alcohol precipitation, collecting precipitate from low to high concentration of alcohol precipitation, and obtaining highland barley beta-glucan crude product;
step ten, filtering: re-suspending the filter residue, stirring again, standing and filtering;
step eleven, dialysis and purification: adding water into the highland barley beta-glucan crude product for re-dissolution, then dialyzing and purifying by using 8-15 ten thousand cellulose dialysis bags, and collecting highland barley beta-glucan pure product solution;
step twelve, drying: drying the highland barley beta-glucan pure product solution to obtain highland barley beta-glucan pure product;
step thirteen, ethanol recovery: and recycling ethanol by adopting an ethanol recycling tower.
In a further preferred embodiment of the present application, the drying temperature in step twelve is 40-60 ℃ and the drying time is 4 hours.
In a further preferred embodiment of the present application, the conditions in the alcohol precipitation process in step nine are: the ethanol concentration is 95%, the edible ethanol with the mass 1-2 times of that of the concentrated solution is added, and the mixture is stirred for 10min and then is kept stand for 30min, and the mixture is filtered after standing.
In a further preferred embodiment of the present application, the centrifugation in step seven uses a decanter centrifuge and a disk centrifuge to centrifuge the extract, respectively.
In a further preferred embodiment of the present application, the pressure of the medium-pressure concentration in the step eight is-0.06 to-0.08 MPa, and the temperature is 60 ℃.
The highland barley beta-glucan is characterized by being prepared by the preparation method of the highland barley beta-glucan of claims 1-5.
The method has the advantages that the characteristics of small molecular polysaccharide are removed by utilizing membrane separation, and meanwhile, the grading alcohol precipitation and dialysis purification treatment are utilized, so that the obtained highland barley beta-glucan has high purity, the process is simple and convenient, the production cost is low, more ethanol can be recycled, water after the ethanol is recovered can be reused, no pollutant is discharged in the whole extraction process, and the environment is favorably protected.
It should be noted that, for simplicity of description, the foregoing embodiments are all illustrated as a series of acts, but it should be understood by those skilled in the art that the present application is not limited by the order of acts, as some steps may be performed in other order or concurrently in accordance with the present application. Further, those skilled in the art will also appreciate that the embodiments described in the specification are all preferred embodiments, and that the acts and modules referred to are not necessarily required for the present application.
In the several embodiments provided by the present application, it should be understood that the disclosed apparatus may be implemented in other manners. For example, the apparatus embodiments described above are merely illustrative, and such partitioning of the above-described elements may be implemented in other manners, e.g., multiple elements or components may be combined or integrated into another system, or some features may be omitted, or not performed. Alternatively, the coupling or communication connection shown or discussed as being between each other may be an indirect coupling or communication connection between devices or elements via some interfaces, which may be in the form of telecommunications or otherwise.
The units described above as separate components may or may not be physically separate, and components shown as units may or may not be physical units, may be located in one place, or may be distributed over a plurality of network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the solution of this embodiment.
The above embodiments are only for illustrating the technical solution of the present application, and not for limiting the scope of the present application. It will be apparent that the described embodiments are merely some, but not all, embodiments of the application. Based on these embodiments, all other embodiments that may be obtained by one of ordinary skill in the art without inventive effort are within the scope of the application. Although the present application has been described in detail with reference to the above embodiments, those skilled in the art may still combine, add or delete features of the embodiments of the present application or make other adjustments according to circumstances without any conflict, so as to obtain different technical solutions without substantially departing from the spirit of the present application, which also falls within the scope of the present application.

Claims (6)

1. The preparation method of highland barley beta-glucan is characterized by comprising the following steps:
step one, raw material preparation: peeling highland barley to obtain highland barley rice;
step two, grinding highland barley: pulverizing semen Avenae Nudae, and sieving with 40-80 mesh sieve to obtain semen Avenae Nudae powder;
step three, alkali liquor extraction: extracting at 30-50deg.C for 2 hr at pH of 7-10;
step four, mixing and adjusting: adding water with a feed liquid ratio of 1g (5-80) mL into highland barley powder, adding alkali liquid to adjust the pH value to 8-10, then adding 20U-2000U of high-temperature amylase, uniformly mixing, and stirring and refluxing for 1-15h at 90-95 ℃;
fifthly, removing protein: adjusting the pH value of the solution obtained in the step four to 3-5, standing, precipitating protein, centrifuging to remove the precipitate, and preparing an extracting solution;
step six, removing small molecule polysaccharide: intercepting the centrifugate by using an organic or inorganic ultrafiltration membrane with molecular weight of 5-10 ten thousand Da, and collecting the permeate;
step seven, centrifugal extraction: centrifuging for 10 minutes by a 4000-turn centrifuge to remove starch and obtain supernatant;
step eight, concentration treatment: concentrating under reduced pressure;
step nine, grading alcohol precipitation: concentrating the permeate, carrying out fractional alcohol precipitation, collecting precipitate from low to high concentration of alcohol precipitation, and obtaining highland barley beta-glucan crude product;
step ten, filtering: re-suspending the filter residue, stirring again, standing and filtering;
step eleven, dialysis and purification: adding water into the highland barley beta-glucan crude product for re-dissolution, and then dialyzing and purifying by using 8-15 ten thousand cellulose dialysis bags, and collecting highland barley beta-glucan pure product solution;
step twelve, drying: drying the highland barley beta-glucan pure product solution to obtain highland barley beta-glucan pure product;
step thirteen, ethanol recovery: and recycling ethanol by adopting an ethanol recycling tower.
2. The method for preparing highland barley beta-glucan according to claim 1, wherein the drying temperature in the step twelve is 40-60 ℃ and the drying time is 4h.
3. The method for preparing highland barley beta-glucan according to claim 1, wherein the conditions of the alcohol precipitation process in the step nine are as follows: the ethanol concentration is 95%, the edible ethanol with the mass 1-2 times of that of the concentrated solution is added, and the mixture is stirred for 10min and then is kept stand for 30min, and the mixture is filtered after standing.
4. The method for preparing highland barley beta-glucan according to claim 1, wherein the centrifugation method in the seventh step is to centrifuge the extract by a decanter centrifuge and a disk centrifuge, respectively.
5. The method for preparing highland barley beta-glucan according to claim 1, wherein the pressure of the concentration in the step eight is-0.06 to-0.08 MPa and the temperature is 60 ℃.
6. The highland barley beta-glucan according to claim 5, wherein the highland barley beta-glucan is produced by the production method of highland barley beta-glucan according to claims 1 to 5.
CN202311170986.2A 2023-09-12 2023-09-12 Highland barley beta-glucan and preparation method thereof Pending CN117088995A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202311170986.2A CN117088995A (en) 2023-09-12 2023-09-12 Highland barley beta-glucan and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202311170986.2A CN117088995A (en) 2023-09-12 2023-09-12 Highland barley beta-glucan and preparation method thereof

Publications (1)

Publication Number Publication Date
CN117088995A true CN117088995A (en) 2023-11-21

Family

ID=88776860

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202311170986.2A Pending CN117088995A (en) 2023-09-12 2023-09-12 Highland barley beta-glucan and preparation method thereof

Country Status (1)

Country Link
CN (1) CN117088995A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115850531A (en) * 2022-11-25 2023-03-28 西藏奇正青稞健康科技有限公司 Preparation method of highland barley beta-glucan with blood sugar reducing function

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115850531A (en) * 2022-11-25 2023-03-28 西藏奇正青稞健康科技有限公司 Preparation method of highland barley beta-glucan with blood sugar reducing function
CN115850531B (en) * 2022-11-25 2024-05-10 西藏奇正青稞健康科技有限公司 Preparation method of highland barley beta-glucan with blood glucose reducing function

Similar Documents

Publication Publication Date Title
CN110272932B (en) Preparation method of ganoderma lucidum spore powder polysaccharide peptide
CN117088995A (en) Highland barley beta-glucan and preparation method thereof
CN101555293B (en) Method for extracting beta- glucosans from bran of corn
CN106117389B (en) Method for extracting and purifying beta-glucan from highland barley grains
CN113186242B (en) Preparation method and application of distillers' grain alcohol-soluble peptide
CN111393540A (en) Preparation method of high-content highland barley β -glucan
CN104757564A (en) Method for preparing dietary fibers by means of peanut shells
CN113244657A (en) Echelon extraction method of functional components of purple highland barley bran
CN104098637B (en) A kind of method of purification feldamycin
CN102180921B (en) Method for extracting high-purity rutin from boxthorn leaves
CN107217080B (en) Method for preparing jerusalem artichoke fructo-oligosaccharide by using immobilized enzyme
US10968470B2 (en) Method for preparing rubusoside
CN111548380A (en) Preparation method of monotropein in morinda officinalis
WO2020042559A1 (en) Method for synchronously extracting lycopene and citrulline from watermelon
CN112876671B (en) Extraction and purification method of plant condensed tannin and application thereof
CN114773495A (en) Method for preparing Fuzhuan tea polysaccharide with blood sugar and blood fat reducing functions
CN107467639A (en) A kind of coproduction prepares oat beta glucan nutrient powder and the method for dietary fiber micro powder
CN103288888A (en) Production method of smallanthus sonchifolius oligosaccharide
CN113057244A (en) Preparation process of tablet candy with immunoregulation effect
CN112293751A (en) Method for extracting soluble dietary fiber by using olive pomace
CN108059686B (en) Method for preparing polysaccharide based on pummelo peel
CN108530557B (en) Laminarin extraction and purification process and application
CN107090012B (en) Method for simultaneously preparing high maltose syrup and protein by using potatoes
CN110522774A (en) The extraction separation method of Akebia trifoliate koiaz Peels polyphenol
CN100449001C (en) Method for extracting effective component of sweet potato leaf stalk vine

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination