CN103288888A - Production method of smallanthus sonchifolius oligosaccharide - Google Patents

Abstract

The invention relates to a production method of smallanthus sonchifolius oligosaccharide. The production method comprises the steps of: washing, sorting, peeling and rapidly chopping a fresh smallanthus sonchifolius raw material, and pulping the chopped material in hot water to realize enzyme inactivation so as to reduce the degradation and decomposition opportunities and the loss of the smallanthus sonchifolius oligosaccharide; filtering to remove insoluble filter residues and fibers to obtain a crude extract of the smallanthus sonchifolius oligosaccharide, and adding a clarifying agent into the crude extract to precipitate and to remove macromolecular components, such as protein, water-soluble enzyme, tannins, pectin and pigment, and part of metal salt ions from the solution; filtering and separating the solution, then precipitating the separated solution by an organic solvent to further remove components, such as protein, tannins, pectin, amino acids, peptides and polysaccharides, and part of metal salt ions in the solution, and recovering the solvent to obtain the high-content smallanthus sonchifolius oligosaccharide.

Description

The production method of yacon oligosaccharide

Technical field:

The invention belongs to biological product and extract the impurity elimination technical field, particularly, relate to a kind of processing method of extracting active oligomeric Nutriflora P in the yacon.

Background technology:

Yacon (Smallanthus sonchifolius) claims saussurea involucrata potato, chrysanthemum potato, inferior dragon fruit or inferior tribute again, English name (yacon), belong to composite family Helianthus plant, originate in the western Andes of South America south of Peru and Bolivia, be aboriginal Indian's tradition tubers food, import Japan, Brazil, Korea S and some European states the eighties in 20 century into, successfully plant in ground big area such as Yunnan Province of China, Sichuan, Guizhou, Fujian, Hainan, Hunan, Hubei, Shandong, Henan, Hebei at present.

Yacon fruit (piece root) is Ipomoea batatas seemingly, but is as far apart as heaven and earth with Ipomoea batatas.Yacon is rich in the necessary amino acid of more than 20 kind of human body and enriches mineral substance, pulp juice is many, and is faint yellow, glittering and translucent, fragrant and sweet crisp and refreshing, mouthfeel is as the water pears, and mouthfeel does not have slag, moisture content 83-95%, soluble solid content 7-10%, contain nutritive elements such as the amino acid of 20 kinds of needed by human and abundant potassium, calcium, zinc, iron, magnesium, crude protein, fat, Mierocrystalline cellulose and oligofructose content height have very high nutritive value; Simultaneously not starch-containing, belong to low calorie fruit, it is edible to be fit to diabetics and slimmer, and the title of " king of underground fruit " is arranged.(dry matter 90% is glucide in DM) to the yacon dry-matter, and wherein 70%-80% is again oligofructose (FOS) sugar, is the highest fruit of oligosaccharide contg in all fruits, and the title of " king of oligofructose " is arranged again.The oligomeric Nutriflora P of yacon (FOS) polymerization degree is mainly between 3～10, and is maximum with the oligofructose content of the polymerization degree 3～5, is up to 17 (GF16).Oligofructose is main active ingredient and the effective constituent in the yacon.Oligofructose (FOS) is not absorption of human body, is the bifidus bacillus multiplicaiton factor, has effects such as enhancing immunity, reducing blood-fat, coordinating intestines and stomach and the absorption of promotion mineral substance.Yacon has conditioning blood, lowering blood glucose, blood fat and cholesterol, the generation of preventing hypertension, diabetes, cardiovascular and cerebrovascular diseases and obesity; Help digest, nurse one's health and improve the undesirable condition of Digestive tract, promote enterogastric peristalsis, relax bowel, eliminate constipation, the control diarrhea promotes the iron calcium absorption, probiotics reduces and the unbalance digestive system that causes in the intestines such as the bifidus bacillus that improvement causes owing to reasons such as rhythm of life anxiety, excessive use microbiotic, is the growth-promoting agent of profitable strain in the intestines; Anti-oxidant, eliminate free radical, reduce or avoid calculous generation; The hypotensive effect of fire falls in the clearing liver detoxifcation, prevents and treats face acne, dark sore, plays the effect of skin maintenance; Improve effects such as body immunity; Belong to medicinal piece root fruit, have very high nutrition and health care, economic worth.

But because fresh yacon stem tuber contains a large amount of moisture (80-90%), fruit matter is crisp, and not anti-storage is ftractureed easily and dewatered transportation and edible inconvenient; Oligomeric Nutriflora P instability in the while yacon, be easy to generate degraded, fresh yacon is after gathering, the part or all of hydrolysis of the very fast generation of oligofructose is decomposed into sucrose, even is reduced to glucose and fructose, its oligofructose of stem tuber of 12 days of lucifuge storage is reduced to 27-39% by 50-62%, lucifuge does not store 6 days oligofructoses and is reduced to 29-44%[He'nan University of Technology journal (natural science edition), 2010,31 (3): 86-90 by 49-58%; Guizhou University of Technology's journal (natural science edition), 2008,37 (4): 175-177; Food research and development, 2006,27 (6): 179-180,188].Heating all can cause the very big reduction of oligosaccharide contg under course of processing mesohigh, high temperature and the acidic conditions, especially high temperature (120 ° of C) autoclaving and baking (200-220 ° of C) and acid (pH3-4.5) condition can cause quick decline [Leshan Teachers College's journal of Nutriflora P content, 2012,27 (5): 17-18].How to preserve effective constituent and activeconstituents-Nutriflora P in the yacon to greatest extent, for the Application and Development of yacon, have very important significance and be worth.

Process for extracting, separating and purifying about the yacon oligofructose, more existing researchs and report, document [Guizhou agricultural sciences 2010,38 (2): 173～176] adopt hot water extraction and microwave-assisted to extract yacon dry powder, the extraction yield of yacon Nutriflora P reaches 62.32% and 61.37% respectively in the extracting solution; Other has document [Chinese pharmacovigilance, 2011,8 (3): 145-147] report directly to adopt microwave-assisted to extract the oligomeric Nutriflora P of yacon dry powder, and the extraction yield of Nutriflora P is 52.85% in the extracting solution.Document [Guizhou University's journal (natural science edition), 2009, V01.26 (4): 100-103] carries out homogenate reusable heat water to fresh yacon in advance to be carried, and it is 6.32% that fresh yacon extracts juicy fruit oligosaccharide extraction yield; Other has document [Guangxi light industry, _ 2010,1:3-4 8.] adopts the fresh yacon oligosaccharide of ultrasonic assisted extraction, the extraction yield of yacon oligosaccharide is 4.257% in the extracting solution, and activated carbon can make oligosaccharide extract the solution decolouring preferably under the concurrent present pH3.5 acidic conditions; The bibliographical information strongly basic anion exchange resin is further arranged, and [China brewages, 2011,3 (total 228): 48-50] and D318 polymeric adsorbent ［ Guangdong chemical industry, 2011,38 (5): 77-78] the yacon oligosaccharide is extracted solution and have decolorizing effect preferably, extraction solution transmittance is greatly improved; But all Nutriflora P in the extracting solution is not carried out purifying, do not have further quality and the content of Nutriflora P to be analyzed comparison yet.Patent CN102219808A has described a kind of organic solvent (acetone, second cyanogen, tetrahydrofuran (THF), 1-propyl alcohol, 2-propyl alcohol) from yacon aqueous extract method of extracting and separating yacon oligofructose miscible with water of utilizing; But since in the yacon except containing oligosaccharide, also contain amounts of protein, pectin, amino acid, polysaccharide, monose, phenolic constituent and metallic element, show that through our test aqueous extract filters very difficult; Product oligosaccharide content is difficult to reach requirement, and color is deep; Organic solvent is difficult for selecting, and water and organic phase layering are difficult to control, must add a large amount of salt loadings, just can make organic phase and water reach the effective layering that separates, and the adding of a large amount of salt brings new trouble for again the separation and purification of Polylevulosan; Consumption of organic solvent is big, and cost height, dissolvent residual are difficult to avoid, and is unfavorable for being applied to food and healthcare products.After patent CN1831014A regulates pH-activated carbon filtration-resin anion(R.A) decolouring-resin cation (R.C.) deliming-ultrafiltration through milk of lime impurity elimination-phosphoric acid after with yacon juice, obtain the yacon oligosaccharide solution of high-content yacon oligosaccharide solution and low levels, obtain the yacon inulin of high-content (90%) yacon oligofructose powder and low levels (25-35%) again through vacuum concentration and lyophilize, again the yacon inulin of low levels is obtained the yacon oligofructose of high-content (90%) with the inulinase fermentation; The operational path complexity, the equipment requirements height, fermentation condition requires harsh, and enzyme liberating product terminal point is difficult to control, and enzymolysis product does not carry out separation and Extraction, and convection drying is difficult to achieve the goal, and also needs single culture to prepare inulinase simultaneously, should use the comparison difficulty.Patent CN102408457A with the yacon warm air drying after the reusable heat water extraction, extracting solution precipitates impurity elimination through high concentration ethanol, concentrate drying obtains thick yacon oligosaccharide, but oligosaccharide content very low (being no more than 40%), separate through silica gel column chromatography again, though can obtain highly purified kestose and tetrose; But be difficult on producing use.

Below as can be seen, oligosaccharide changes greatly in the yacon, and the extraction and purification process method of having reported and having applied for a patent at present is also not really desirable.In addition, because the yacon stem tuber is interior except containing oligofructose, also contain glucides such as fructose, glucose, sucrose and phenolic acid such as chlorogenic acid, coffic acid, forulic acid, Protocatechuic Acid etc., organic composition and inorganic salt compositions such as flavonoid, terpene, volatile oil, polysaccharide, amino acid, protein, pectin, fat, Mierocrystalline cellulose, pigment, potassium, calcium, zinc, iron, magnesium also have a large amount of polyphenoloxidase, lytic enzyme to exist simultaneously.The existence of liposoluble ingredient and polyphenoloxidase can produce brown stain in the leaching process, lytic enzymes etc. can make oligomeric Nutriflora P degrade, high temperature and long-time heating can make amino acid and sugar that a series of variations such as Maillard reaction, hydrolysis take place, and have a strong impact on content, output, quality and the color and luster of yacon oligosaccharide.Directly adopt with the miscible organic solvent extraction yacon of water in oligosaccharide, fat-soluble component can not be removed on the one hand, water soluble component is removed and not exclusively, is influenced color and the content of levan oligo-saccharide, has organic solvent residual on the other hand.The ethanol sedimentation impurity elimination, though can remove most of water-soluble impurity, fat-soluble component and pigment and micromolecular water soluble components are difficult to again remove.Though can making macromole reach preferably with small molecules, separates by ultra-filtration membrane, but it is bigger to make small molecule monomer and oligosaccharide carry out separating difficulty, owing to the influence of a large amount of pectin and liposoluble ingredient in the yacon, be easy to make film to stop up simultaneously, bring bigger difficulty for cleaning and the replacing of film.Ion exchange resin has reasonable effect for the decolouring of yacon oligosaccharide and the removal of ash content, but wants other composition of place to go and carry out purifying, does not have much effects.Polymeric adsorbent is for the adsorption of having of fat-soluble component in the yacon and look reasonable, but wants the water-soluble coexistence composition difficulty relatively in the yacon oligosaccharide of place to go.Adopt column chromatography and above several different methods to cooperate, though can obtain more highly purified yacon oligosaccharide, one side technology more complicated, cost is too high on the other hand, is unfavorable for application and the exploitation of yacon oligosaccharide.Though enzymatic hydrolysis and fermentation can improve productive rate and the content of yacon oligosaccharide, make a part of yacon water-insoluble fiber, polysaccharide and pectin degrading be the fruit oligosaccharide, but the conditional request harshness also can be degraded to monose with part fruit oligosaccharide, the above purifying difficult problem of same existence simultaneously.

Summary of the invention

The present invention is intended to the above-mentioned deficiency at prior art, and a kind of high-quality yacon oligosaccharide that can be applied to food, healthcare products, beverage is provided, and preserves the simplified method of Nutriflora P composition in the yacon raw material as far as possible.The fresh yacon that employing will now excavate or refrigerate is cleaned, and rejects the rotten raw material that goes mouldy, and peeling is shredded fast to add in the hot water and pulled an oar, and makes enzyme deactivation, to reduce degraded and the decomposition of yacon oligosaccharide, reduces the loss of yacon oligosaccharide; After Yacon pulp removes by filter insoluble filter residue and fiber, add finings, precipitation is removed macromolecular components and part metals salt ions such as protein in the solution, water-soluble enzyme, tannin, pectin, pigment; Further remove composition and part metals salt ions such as protein in the solution, tannin, pectin, amino acid, polypeptide, polysaccharide again with ethanol sedimentation, thereby obtain high-content yacon oligosaccharide.

In order to realize above-mentioned purpose of the present invention, the present invention has adopted following technical scheme:

Yacon oligosaccharide production method, the fresh yacon that enchashment is dug or refrigerated is cleaned, the rejecting rotten raw material that goes mouldy, peeling, pull an oar in 60-100 ° of C hot water of chopping adding fast and grind, after removing by filter insoluble filter residue and fiber, filtrate is heated to 30-90 ° of C, stir the finings solution that adds filtrate volume 3-12%, staticly settle, removing by filter precipitation, filtrate is decompressed under 30-80 ° of C condition-and 0.05-0.08MPa is concentrated into the 1/2-1/20 volume, stirs the organic solvent miscible with water that adds 1-7 times of volume of concentrated solution, staticly settle, removing by filter precipitation, filtrate is decompressed under 30-70 ° of C condition-and 0.05-0.08MPa is concentrated into and does or be concentrated into no ethanol final vacuum drying, lyophilize, spraying drying is pulverized and is obtained high-content yacon oligosaccharide.

Described production method, hot water are distilled water or deionized water.Should not use tap water, cause the metal ion ash content to exceed standard easily.Temperature is unsuitable too high or too low, the low excessively not purpose of ambassador's yacon enzyme denaturation inactivation that reaches, and the too high yacon glycan of accelerating easily again decomposes.

Described production method, the 1-20 of the amount yacon raw material of hot water doubly.Very few yacon oligosaccharide extracts not exclusively, crosses to cause the waste of subsequent technique material usage at most, and cost is increased.

Described production method is filtered and is adopted natural filtration or vacuum filtration or filter press or centrifuging.

Described method, described finings are any one or two kinds that include but not limited in 101 finingss, KBT-YSC, KBT-GZC, ZTC1+1 finings, chitosan, KBT-FJC, KBT-HJC, HD003, SX-865, XM-118, NCA, KJ-1, DHG, BD, CTS, CZ-1, HD003, JH, KJ, NVP, PF-II, G409, section sun 1+1, Han Weisite, JA, GZB, medicine holy 1+1, XYZ1+1, VA debt VB, KOLORFINE, the cationic polyacrylamide.More common with 101 finingss, ZTC1+1 finings, chitosan, BD, JA finings.

It is more reasonable that the 1/2-1/20 that described method, described settled solution are concentrated into original volume adds organic solvent again, can greatly reduce consumption of organic solvent, reaches optimal effectiveness.But excessive concentration is unfavorable for the organic solvent deposit impurity elimination, occurs the thickness layering easily; Cross that low then consumption of organic solvent is excessive.

Described method, described organic solvent be include but not limited to alcohol, methyl alcohol, acetone etc. can with the mixed solvent of any a kind or 2-3 kind of the miscible solvent of water.Take all factors into consideration from security and raw material sources, best with ethanol.

Described production method, drying means adopts vacuum-drying or lyophilize or spraying drying or warm air drying.Vacuum-drying is generally controlled at 60 ° below the C.Drying temperature is unsuitable too high, and bonding appears in too high back easily, is unfavorable for the pulverizing powdery of product, and causes color burn.

Compared with prior art, the present invention possesses following excellent benefit:

This invention is at first shredded fresh yacon fast to add in the hot water and is pulled an oar, and makes enzyme deactivation, to reduce degraded and the decomposition of yacon oligosaccharide, reduces the loss of yacon oligosaccharide; After removing by filter insoluble filter residue and fiber, directly add finings, remove macromolecular components and part metals salt ions such as protein in the solution, water-soluble enzyme, tannin, pectin, pigment with precipitation; Further remove composition and part metals salt ions such as protein in the solution, tannin, pectin, amino acid, polypeptide, polysaccharide again with ethanol sedimentation, thereby obtain high-quality yacon oligosaccharide.

Description of drawings:

Fig. 1 is yacon oligosaccharide technological process of production figure of the present invention.

Embodiment:

Below in conjunction with accompanying drawing, further specify essentiality content of the present invention with the specific embodiment of the invention, but do not limit the present invention with this.

Embodiment 1:

Get fresh yacon piece root, choose the rotten fruit that goes rotten, clear water is cleaned, dry moisture, take by weighing 2000g, the crust of pruning, be chopped into bulk in small, broken bits, be heated to immediately in the 2000mL deionized water of 90 ° of C, making beating grinds, and filters, and with 500mL deionized water wash pomace 2 times, discard filter residue, merging filtrate gets muddy shape thickness extracting solution 4000mL, be heated to 50 ° of C, stir and slowly add the 1% weight concentration chitosan solution that 300mL prepares in advance, continuation stirring insulation 30min, cooling, placed 3 hours, remove by filter precipitation, get light yellow clarification extracting solution, be heated to 50 ° of C, be decompressed to-concentrate under the 0.08MPa condition and be recycled to 300mL, stir adding 1500mL ethanol, place clarification 6 hours, remove by filter precipitation, get extraction using alcohol solution, be heated to 40 ° of C, be decompressed to-concentrate under the 0.08MPa condition and be recycled to driedly, get faint yellow yacon oligosaccharide 128g.Sulfuric acid-phynol method is measured total sugar content, and the DNS method is measured reducing sugar content, calculates fruit oligosaccharide content 82.5%; High temperature ash purification determining ash oontent 2.3%.Production technique is referring to Fig. 1.

Embodiment 2:

Get fresh yacon piece root, choose the rotten fruit that goes rotten, clear water is cleaned, dry moisture, take by weighing 2000g, the crust of pruning, be chopped into bulk in small, broken bits, be heated in the 2000mL distilled water of 85 ° of C levigate making beating, press filtration, and with 500mL distilled water wash pomace 2 times, discard filter residue, merging filtrate gets muddy shape thickness yacon extracting solution 4000mL, be heated to 80 ° of C, stir the 1% weight concentration ZTC1+1II type finings B solution that the slow 200mL of adding prepares, continue to stir insulation 1 hour, slowly add the 1% weight concentration ZTC1+1II type finings A solution that 100mL prepares again, continued insulated and stirred 15 minutes, stop to stir, cooling was placed 10 hours, the sucking-off supernatant liquid, lower sediment centrifuging, filtrate and supernatant liquid merge, and are decompressed to-0.08MPa, be heated to 60 ° of C vacuum concentration and be recycled to 300mL, stir adding 1200mL ethanol, place clarification 4 hours, remove by filter precipitation, get extraction using alcohol solution, be heated to 40 ° of C, be decompressed to-concentrate under the 0.08MPa condition and be recycled to driedly, get yellow-white yacon oligosaccharide 131g.Sulfuric acid-phynol method is measured total sugar content, and the DNS method is measured reducing sugar content, calculates fruit oligosaccharide content 83.7%; High temperature ash purification determining ash oontent 2.5%.

Embodiment 3:

Get fresh yacon piece root, choose the rotten fruit that goes rotten, flushing with clean water is clean, dry exterior skin moisture, take by weighing 1000g, the crust of pruning, be chopped into bulk in small, broken bits, be heated to immediately in the 1000mL distilled water of 80 ° of C, levigate making beating is filtered, and with 300mL distilled water wash pomace 2 times, discard filter residue, merging filtrate gets muddy shape thickness yacon extracting solution 2000mL, be heated to 60 ° of C, stir 1% weight concentration, the 101 fruit juice clarifier A solution that the slow 120mL of adding prepares, continue to stir insulation 1 hour, slowly add 1% weight concentration, the 101 fruit juice clarifier B solution that 60mL prepares again, continued insulated and stirred 20 minutes, stop to stir, cooling was placed 8 hours, extract supernatant liquid, precipitation is removed in lower sediment centrifuging, and merging filtrate and supernatant liquid are heated to 40 ° of C, be decompressed to-the 0.08MPa vacuum concentration is recycled to 200mL, stir adding 1200mL ethanol, place clarification 3 hours, remove by filter precipitation, get extraction using alcohol solution, be heated to 40 ° of C, be decompressed to-concentrate under the 0.08MPa condition and be recycled to driedly, get yellow yacon oligosaccharide 62g.Sulfuric acid-phynol method is measured total sugar content, and the DNS method is measured reducing sugar content, and calculating fruit oligosaccharide content is 80.6%; High temperature ash purification determining ash oontent 2.8%.

Embodiment 4:

Get fresh yacon piece root, choose the rotten fruit that goes rotten, flushing with clean water is clean, dry exterior skin moisture, take by weighing 1500g, the crust of pruning, be chopped into bulk in small, broken bits, be heated to immediately in the 1500mL distilled water of 90 ° of C, levigate making beating is filtered, and with 400mL distilled water wash pomace 2 times, discard filter residue, merging filtrate gets muddy shape thickness yacon extracting solution 3000mL, be heated to 70 ° of C, stir the sun 1+1 of the 1% weight concentration section finings A solution that the slow 150mL of adding prepares, continuation is stirred and is incubated 1.5 hours, slowly adds the sun 1+1 of the 1% weight concentration section finings B solution that 75mL prepares again, continued insulated and stirred 30 minutes, stop to stir, cooling was placed 7 hours, extract supernatant liquid, the lower sediment Plate Filtration is removed precipitation, and merging filtrate and supernatant liquid are heated to 40 ° of C, be decompressed to-the 0.08MPa vacuum concentration is recycled to 300mL, stir adding 1200mL ethanol, place clarification 3 hours, remove by filter precipitation, get extraction using alcohol solution, be heated to 40 ° of C, be decompressed to-concentrate under the 0.08MPa condition and be recycled to driedly, get yellow yacon oligosaccharide 103g.Sulfuric acid-phynol method is measured total sugar content, and the DNS method is measured reducing sugar content, and calculating fruit oligosaccharide content is 81.5%; High temperature ash purification determining ash oontent 2.7%.

Embodiment 5:

Get fresh yacon piece root, reject the rotten fruit that goes rotten, flushing with clean water is clean, dry exterior skin moisture, take by weighing 1500g, the crust of pruning, be chopped into bulk in small, broken bits, be heated to immediately in the 1500mL deionized water of 85 ° of C, pour in the pulp machine and pull an oar, filter, and with the cold pure water of 400mL washing pomace 2 times, discard filter residue, merging filtrate, get the thick fruit juice 3000mL of muddy shape thickness, be heated to 75 ° of C, stir the 1% weight concentration BD finings B solution that the slow 180mL of adding prepares, continue to stir insulation 1.5 hours, slowly add the 1% weight concentration BD finings A solution that 90mL prepares again, continue insulated and stirred 20 minutes, and stopped to stir cooling, placed 6 hours, extract supernatant liquid, the centrifugal precipitation of removing of lower sediment, merging filtrate and supernatant liquid, be heated to 50 ° of C, be decompressed to-the 0.08MPa vacuum concentration is recycled to 300mL, stirs to add 1200mL ethanol, places clarification 3 hours, remove by filter precipitation, extraction using alcohol solution, be heated to 40 ° of C, be decompressed to-concentrate under the 0.08MPa condition and be recycled to no ethanol and distinguish the flavor of, spraying drying gets yellow yacon oligosaccharide 102g.Sulfuric acid-phynol method is measured total sugar content, and the DNS method is measured reducing sugar content, and calculating fruit oligosaccharide content is 81.4%; High temperature ash purification determining ash oontent 2.6%.

Embodiment 6:

Get fresh yacon piece root, reject the rotten fruit that goes rotten, flushing with clean water is clean, dry exterior skin moisture, take by weighing 1000g, the crust of pruning, be chopped into bulk in small, broken bits, be heated in the 1000mL distilled water of 95 ° of C levigate making beating immediately, filter, and with 300mL distilled water wash pomace 2 times, discard filter residue, merging filtrate, get muddy shape thickness yacon extracting solution 2000mL, be heated to 50 ° of C, stir the 1% weight concentration JA finings solution that the slow 200mL of adding prepares, continue to stir insulation 1 hour, stop to stir, cooling was placed 5 hours, extracted supernatant liquid, the lower sediment Plate Filtration is removed precipitation, merging filtrate and supernatant liquid are heated to 50 ° of C, be decompressed to-the 0.08MPa vacuum concentration is recycled to 200mL, stir and add 1000mL ethanol, place clarification 3 hours, remove by filter precipitation, get extraction using alcohol solution, be heated to 40 ° of C, be decompressed to-concentrate under the 0.08MPa condition and be recycled to no ethanol and distinguish the flavor of, spraying drying gets yellow yacon oligosaccharide 69g.Sulfuric acid-phynol method is measured total sugar content, and the DNS method is measured reducing sugar content, and calculating fruit oligosaccharide content is 80.9%; High temperature ash purification determining ash oontent 2.4%.

Claims (10)

1. yacon oligosaccharide production method, getting yacon cleans, rejecting is gone mouldy rotten, peeling, pull an oar in 60-100 ° of C hot water of chopping adding fast and grind, after removing by filter insoluble filter residue and fiber, filtrate is heated to 30-90 ° of C, stir the finings solution that adds filtrate volume 3-12%, staticly settle, remove by filter precipitation, filtrate is decompressed under 30-80 ° of C condition-0.05-0.08MPa is concentrated into the 1/2-1/20 volume, stir add 1-7 times of volume of concentrated solution volume can be miscible with water organic solvent, staticly settle, remove by filter precipitation, filtrate is being decompressed under the 30-70 ° of C condition-0.05-0.08MPa be concentrated into do or be concentrated into no ethanol after dry, pulverizing obtains the yacon oligosaccharide.

2. production method as claimed in claim 1 is characterized in that the yacon that the yacon enchashment is excavated or refrigerated.

3. production method as claimed in claim 1 is characterized in that hot water is distilled water or deionized water.

4. production method as claimed in claim 1, the amount that it is characterized in that hot water be the yacon raw material 1-20 doubly.

5. production method as claimed in claim 1 is characterized in that described finings is any one or two kinds in 101 finingss, KBT-YSC, KBT-GZC, ZTC1+1 finings, chitosan, KBT-FJC, KBT-HJC, HD003, SX-865, XM-118, NCA, KJ-1, DHG, BD, CTS, CZ-1, HD003, JH, KJ, NVP, PF-II, G409, section sun 1+1, Han Weisite, JA, GZB, medicine holy 1+1, XYZ1+1, VA debt VB, KOLORFINE, the cationic polyacrylamide.

6. as claim 1 or 5 described production methods, it is characterized in that described finings is 101 finingss or ZTC1+1 finings or chitosan or BD or JA finings.

7. production method as claimed in claim 1 is characterized in that filtering employing natural filtration or vacuum filtration or filter press or centrifuging.

8. the method for claim 1 is characterized in that described organic solvent is etoh solvent or methyl alcohol or acetone any a kind or 2-3 kind that can be miscible with water.

9. as claim 1 or 8 described methods, it is characterized in that described organic solvent is ethanol.

10. production method as claimed in claim 1 is characterized in that drying means adopts vacuum-drying or lyophilize or spraying drying or warm air drying.

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