CN117084086A - Application of p-coumarate methyl ester in promoting root growth and reducing nitrous oxide emission - Google Patents
Application of p-coumarate methyl ester in promoting root growth and reducing nitrous oxide emission Download PDFInfo
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- CN117084086A CN117084086A CN202311075096.3A CN202311075096A CN117084086A CN 117084086 A CN117084086 A CN 117084086A CN 202311075096 A CN202311075096 A CN 202311075096A CN 117084086 A CN117084086 A CN 117084086A
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- NITWSHWHQAQBAW-QPJJXVBHSA-N (E)-4-coumaric acid methyl ester Chemical compound COC(=O)\C=C\C1=CC=C(O)C=C1 NITWSHWHQAQBAW-QPJJXVBHSA-N 0.000 title claims abstract description 65
- GQPLMRYTRLFLPF-UHFFFAOYSA-N Nitrous Oxide Chemical compound [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 title claims abstract description 18
- 230000002786 root growth Effects 0.000 title claims abstract description 18
- 230000001737 promoting effect Effects 0.000 title claims abstract description 13
- 239000001272 nitrous oxide Substances 0.000 title claims abstract description 9
- 239000002689 soil Substances 0.000 claims abstract description 45
- 241000208125 Nicotiana Species 0.000 claims abstract description 37
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims abstract description 37
- 239000005431 greenhouse gas Substances 0.000 claims abstract description 8
- 239000003630 growth substance Substances 0.000 claims abstract description 4
- 241000196324 Embryophyta Species 0.000 claims description 17
- 239000000618 nitrogen fertilizer Substances 0.000 claims description 8
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 2
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 2
- NGSWKAQJJWESNS-ZZXKWVIFSA-M trans-4-coumarate Chemical compound OC1=CC=C(\C=C\C([O-])=O)C=C1 NGSWKAQJJWESNS-ZZXKWVIFSA-M 0.000 claims description 2
- 239000001166 ammonium sulphate Substances 0.000 claims 1
- 230000012010 growth Effects 0.000 abstract description 17
- 241001002356 Valeriana edulis Species 0.000 abstract description 7
- 238000000034 method Methods 0.000 abstract description 5
- 238000011161 development Methods 0.000 abstract description 4
- 230000006870 function Effects 0.000 abstract description 3
- 230000005764 inhibitory process Effects 0.000 abstract description 3
- 230000001276 controlling effect Effects 0.000 abstract description 2
- 238000005265 energy consumption Methods 0.000 abstract description 2
- 239000003337 fertilizer Substances 0.000 abstract description 2
- 230000010354 integration Effects 0.000 abstract description 2
- 230000000813 microbial effect Effects 0.000 abstract description 2
- 230000001105 regulatory effect Effects 0.000 abstract description 2
- -1 methyl p-coumarate compound Chemical class 0.000 abstract 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 abstract 1
- 239000003112 inhibitor Substances 0.000 description 17
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 16
- 238000011282 treatment Methods 0.000 description 10
- 229910052757 nitrogen Inorganic materials 0.000 description 9
- 239000007789 gas Substances 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- 238000012360 testing method Methods 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- 230000009467 reduction Effects 0.000 description 5
- 230000008635 plant growth Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 240000006394 Sorghum bicolor Species 0.000 description 3
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 3
- 230000033228 biological regulation Effects 0.000 description 3
- 238000013400 design of experiment Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- QOHGMEYPUKSMST-UHFFFAOYSA-N methyl 2-(4-hydroxyphenyl)propanoate Chemical compound COC(=O)C(C)C1=CC=C(O)C=C1 QOHGMEYPUKSMST-UHFFFAOYSA-N 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 2
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 241000219195 Arabidopsis thaliana Species 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 1
- 244000124853 Perilla frutescens Species 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 1
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- WBCMGDNFDRNGGZ-ACNVUDSMSA-N coumarate Natural products COC(=O)C1=CO[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H]3[C@@H]1C=C[C@]34OC(=O)C(=C4)[C@H](C)OC(=O)C=Cc5ccc(O)cc5 WBCMGDNFDRNGGZ-ACNVUDSMSA-N 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- FPVGTPBMTFTMRT-UHFFFAOYSA-L disodium;2-amino-5-[(4-sulfonatophenyl)diazenyl]benzenesulfonate Chemical compound [Na+].[Na+].C1=C(S([O-])(=O)=O)C(N)=CC=C1N=NC1=CC=C(S([O-])(=O)=O)C=C1 FPVGTPBMTFTMRT-UHFFFAOYSA-L 0.000 description 1
- 230000003828 downregulation Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000019233 fast yellow AB Nutrition 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 235000020778 linoleic acid Nutrition 0.000 description 1
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 1
- 229960004488 linolenic acid Drugs 0.000 description 1
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000000442 meristematic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000001546 nitrifying effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000008447 perception Effects 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000003642 reactive oxygen metabolite Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/45—Tobacco
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Application of methyl p-coumarate in promoting root growth and reducing nitrous oxide emission, the methyl p-coumarate compound provided by the invention can collect nitrification inhibition and N 2 O reduces the emission and promotes the growth of tobacco root systems to be multifunctional, thereby realizing the integration of the functions. Methyl p-coumarate not only can reduce the greenhouse gas N of tobacco planting soil by regulating and controlling the microbial activity of the soil 2 O is discharged, and the growth of tobacco root systems can be directly promoted. The method provides possibility for replacing various synergists/stimulators for adding the methyl coumarate in the development of novel green nitrogenous fertilizers and tobacco seedling growth regulators, and reduces energy consumption and cost.
Description
Technical Field
The invention belongs to the technical field of plant root growth regulation and greenhouse gas emission reduction, and in particular relates to a biological nitrification inhibitor for methyl coumarate in promoting tobacco root growth and reducing tobacco planting soil N 2 Application in O emission.
Background
Nitrogen is an important nutrient element in the growth and development process of tobacco, and the application of nitrogen fertilizer directly influences the yield and quality of tobacco. How to improve the utilization rate of the nitrogen fertilizer of the tobacco, reduce the environmental impact and promote the growth of the flue-cured tobacco has become a key problem of synergy and emission reduction of the flue-cured tobacco. The nitrification-denitrification process is closely related to the loss and utilization of soil nitrogen, and chemical nitrification inhibitors have been used to reduce the loss of farmland soil nitrogen due to nitrification. In recent years, natural-source and environment-friendly biological nitrification inhibitors are a new strategy for synergism and emission reduction of agricultural nitrogen.
Nitrous oxide (N) 2 O) is a greenhouse gas having a global warming potential greater than CO 2 298 times higher. N can be generated by nitrification and denitrification in soil 2 O. Although some biological nitrification inhibitors can inhibit soil nitrification processes and N 2 O discharges, but due to different chemical structures of different inhibitors, some of the inhibitors can be used as easily-decomposed carbon sources to provide substrates for denitrifying microorganisms in soil, so that the denitrifying activity of the microorganisms is promoted, and finally N in the soil is caused 2 The O emissions increase. For example, on soil with strong nitrification, two kinds of soil are usedLinear fatty acid biological nitrification inhibitor, linolenic acid and linoleic acid can obviously increase soil N under high concentration addition 2 O is discharged. This pair of greenhouse gases N 2 The promoting effect of O emissions is to be avoided as much as possible in biological nitrification inhibitor applications.
In addition, biological nitrification inhibitor can reduce nitrogen loss to increase ammonium nitrogen in soil, but excessive ammonium in soil can stress plants to inhibit root growth. Therefore, biological nitrification inhibitors reduce nitrogen loss and do not necessarily mean a promoting effect on plant growth. Recent researches find that the biological nitrification inhibitor can directly regulate physiological processes in plants to inhibit root growth. For example, the sorghum-derived biological nitrification inhibitor methyl p-hydroxyphenylpropionate (MHPP) remarkably inhibits root growth of model plant Arabidopsis thaliana and medicinal plant perilla. MHPP affects root tip auxin perception and response by inducing nitric oxide accumulation and elevated levels of reactive oxygen species, thereby inhibiting division of root tip meristematic cells and primary root growth. Another biological nitrification inhibitor of sorghum quinone (sorgo) secreted by sorghum inhibits photosynthesis in plants. These direct negative regulation of plant growth can affect future development and application of biological nitrification inhibitors, limiting the practical use of biological nitrification inhibitors.
Methyl p-coumarate is a biological nitrification inhibitor that was previously found in root tissues. However, in the past, attention has been paid mainly to the effect of methyl p-coumarate on the nitrification activity in a pure culture system of nitrifying strains, and the reduction of greenhouse gas N in methyl p-coumarate 2 The use of O emissions and regulation of tobacco root growth has not been disclosed. In flue-cured tobacco production, screening simultaneously reduces greenhouse gas N 2 O discharges plant source functional substances which are directly beneficial to plant growth, greatly promotes the research and development of green novel efficient nitrogenous fertilizer and root and seedling promoting agent for flue-cured tobacco, and has important practical significance for sustainable development of flue-cured tobacco industry.
Disclosure of Invention
The technical problems to be solved are as follows: aiming at the technical problems, the invention provides an application of methyl p-coumarate in promoting root growth and reducing nitrous oxide emission, which can collectNitrification inhibition, soil N 2 O reduces the emission and regulates the growth of the root system of the tobacco seedling into a whole, thereby realizing the integration of the functions. The method provides a simple, green and effective solution for improving the utilization rate of nitrogen fertilizer and reducing the loss of nitrogen in flue-cured tobacco production and simultaneously promoting the growth and stress resistance of tobacco root systems.
The technical scheme is as follows: the application of methyl p-coumarate in promoting plant root growth or reducing nitrous oxide emission in tobacco planting soil chamber is provided.
The judging indexes of the root system growth are the length of the main root and the quantity of the lateral roots of the plant.
The addition concentration of the p-coumarate methyl ester in promoting the growth of plant root systems is 5-40 mu M.
The plant is tobacco.
The addition concentration of the p-coumarate methyl ester in reducing the emission of yellow soil greenhouse gas nitrous oxide is 10-500 mu M.
The tobacco planting soil is yellow soil.
The p-coumarate methyl ester and ammonium sulfate are mixed to prepare a mixed solution, and then the mixed solution is applied to soil with the application amount of 50-200 mg.N.kg -1 Soil.
Application of methyl p-coumarate in preparing tobacco seedling growth regulator is provided.
Application of methyl p-coumarate in preparing tobacco nitrogenous fertilizer products.
The beneficial effects are that: the invention provides a multidirectional regulation and control effect on methyl coumarate, which not only can improve the utilization rate of nitrogen fertilizer and reduce N by regulating and controlling the microbial activity of soil 2 O discharges, and can also directly promote the growth of main roots and the quantity of lateral roots of tobacco seedlings under proper addition concentration. The application field of the methyl p-coumarate is further widened, and the industrial application value of the methyl p-coumarate is improved. The p-coumarate has single molecule and multiple functions, and one substance can play a combined role of adding various fertilizer synergists and stimulators. The novel nitrogen fertilizer or the growth regulator can replace various synergists/stimulators to be added in the preparation of agricultural production, so that the types of the synergists/stimulators are reduced, the energy consumption and the cost are reduced, and the potential threat to the environment caused by the addition of the various synergists/stimulators is reduced. For a pair ofThe methyl coumarate can promote the growth of tobacco root systems, can be used as a synergist or a stimulator to be sprayed on plants when being applied, and can be applied to soil instead of other nitrogen synergists, thereby saving more labor.
Drawings
FIG. 1 shows the effect of various amounts of added methyl coumarate on tobacco root growth.
FIG. 2 shows the effect of varying amounts of added methyl coumarate on tobacco lateral root growth.
FIG. 3 shows P.coumarate N in tobacco planting soil of Qian southwest 2 Influence of O emissions.
Detailed Description
Example 1: influence of different addition amounts on the growth of main roots of tobacco seedlings by methyl coumarate
1. Design of experiment
1.1 test plants: the plant material is tobacco K326.
1.2 test agent: p-coumaric acid methyl ester (C) 10 H 10 O 3 )。
1.3 experimental treatment: the experiment set up 6 final concentrations of methyl p-coumarate, respectively: 0. Mu.M, 5. Mu.M, 10. Mu.M, 20. Mu.M, 40. Mu.M, 100. Mu.M.
1.4 experimental procedure:
tobacco seeds were placed in sterilized 1.5mL centrifuge tubes and 1mL of 10% H was added 2 O 2 10 mu L of sodium dodecyl sulfate, uniformly swirling by a vortex meter, ensuring complete seed sterilization, keeping for 15min, spraying 75% alcohol on the surface of a centrifuge tube, sterilizing, and transferring into an ultra-clean bench. The seeds are washed 5-7 times by sucking sterilized water by a pipette until the seeds are washed. Then, an appropriate amount of 0.1% agarose solution was added to suspend the seeds in the agarose solution. Seeds were sown onto a conventional germination culture MS matrix (Sigma-Aldrich, pH adjusted to 5.80). The substrate plate was sealed with a Parafilm and placed vertically in a light incubator to allow root growth vertically downward along the substrate surface. The photoperiod of the culture room is 16h/8h, the temperature (23+/-1) DEG C and the illumination intensity is 100 mu mol.m -2 ·s -1 . After the tobacco seeds germinate on a conventional culture medium for 7 days, taking tobacco seedlings with uniform growth vigor, and transferring the tobacco seedlings to a medium treated by methyl coumarateThe growth was continued on, and the main root length and lateral root number of tobacco were measured on day 5 of culture, with 15 replicates per treatment. Elongation of main root: the initial root length was marked at the bottom of the substrate after seedling transplanting and the net growth was measured with a ruler at day 5 after seedling transplanting for 5 days. The elongation of the main root refers to the newly increased length of the main root after moving to the treatment plate.
1.5 experimental results
The addition of 5. Mu.M, 10. Mu.M, 40. Mu.M and 60. Mu.M of methyl p-coumarate significantly promoted the growth of the main roots of tobacco K326 at 37.2% -74.5% compared to the control without added methyl p-coumarate, with 10. Mu.M being the most effective (FIG. 1). In addition, the promotion rate tends to decrease with increasing concentration, and the growth of the main root of K326 is inhibited at 100. Mu.M.
Example 2: influence of different addition amounts on growth of lateral roots of tobacco seedlings by methyl coumarate
2. Design of experiment
2.1 experimental procedure: the test plants, test reagents, experimental treatments and experimental procedures were as in example 1.
Measurement of the number of side roots: after transplanting seedlings to MS culture medium treated by methyl coumarate with different concentrations for 5 days, the number of mature primary lateral roots (the length of the lateral roots is more than 0.5 mm) is counted by means of naked eyes observation with the help of a measuring scale. Individuals of plants without lateral roots were counted as zero.
2.2 experimental results
The 10. Mu.M, 20. Mu.M and 40. Mu.M methyl p-coumarate treatments all significantly promoted tobacco K326 primary side root number by about 15-30% compared to the no added methyl p-coumarate control (FIG. 2). With increasing concentration, the promoting effect becomes smaller gradually, and the growth of lateral root is inhibited at 100 mu M. The addition of 10-20 mu M methyl coumarate has better promotion effect on tobacco K326 main root and lateral root by combining the result of main root.
Example 3: different addition amounts of p-coumarate methyl p-tobacco planting soil N 2 Influence of O emissions
3. Design of experiment
3.1 test soil: the acid yellow soil tested was obtained from Xingzheng city, miao nationality, southwest, guizhou province. Collecting from the surface layer of cultivated land, removing impurities, air drying, sieving with 2mm sieve, and storing at room temperature. The physicochemical properties of the soil are shown in Table 1.
TABLE 1 basic physicochemical Properties of test soil
3.2 experimental procedure: 100mL glass flasks were used for culture, 5 treatments were set, and 3 replicates were set for each treatment. 10g of dry soil was weighed and placed in a flask, and suction (NH 4 ) 2 SO 4 And applying the methyl coumarate biological nitrification inhibitor treatment liquid at different concentrations to uniformly mix the treatment liquid into the soil as much as possible, wherein the water content of the soil is adjusted to 60% WHC, and the concentration (NH 4 ) 2 SO 4 The concentration is 100mg N kg -1 The final concentration of methyl p-coumarate in the soil was 0, 10, 50, 100, 500 μm, respectively. Sealing with rubber plug, culturing in incubator at 25deg.C under shade, collecting gas on 1 st, 2 nd, 3 rd, 4 th, 5 th, 6 th, 7 th, 10 th, 14 th and 21 th days after adding treatment liquid, sealing for 23 hr after exhausting glass bottle before sampling, repeatedly extracting 3 times with 25mL syringe injector, collecting 20mL gas, and injecting into vacuum bottle for measuring N 2 O concentration.
Soil N 2 O production rate:
F＝(M/V m )×(dc/dt)×273/(273+T)×V/m
wherein: f represents the discharge rate of the gas, N 2 O-N, μg/(kg.h); m is N 2 O molar mass, 28g/mol; v (V) m Is the molar volume of the gas, 22.4L/mol; dc/dt is the increase of gas concentration in culture flask in unit time, 10 -9 /h; t is the temperature during culture and is at the temperature of DEG C; v is the effective space volume of the gas in the culture flask, L; m is the weight of the baked soil in the culture flask, and kg. N (N) 2 The O gas emission accumulation amount can be calculated by time-weighting the average generation rate of the gas for each period of time according to the rate generation formula described above.
3.3 experimental results
As shown in FIG. 3, after 21 days of culture, various concentrations of methyl p-coumarateTreatment of tobacco-planting soil N 2 The O emission is obviously inhibited. With the increase of the concentration of the methyl p-coumarate, the p-tobacco planting soil N 2 The degree of inhibition of O emissions increases gradually. At 10. Mu.M, p-coumarate methyl ester was able to reduce 16.9% N 2 O emission, at an addition of 100. Mu.M, was reduced by 53.3% of the soil N 2 O is discharged; when the addition concentration is 500 mu M, the soil N 2 The O emission reduction range is 62.8%.
Claims (9)
1. The application of methyl p-coumarate in promoting plant root growth or reducing nitrous oxide emission in tobacco planting soil chamber is provided.
2. The use according to claim 1, wherein the root growth judgment index is the main root length and the lateral root number of the plant.
3. The use according to claim 1, wherein the concentration of p-coumarate added to promote plant root growth is 5-40 μm.
4. The use according to claim 1, wherein the plant is tobacco.
5. The use according to claim 1, wherein the methyl p-coumarate is added at a concentration of 10-500 μm for reducing the emission of yellow soil greenhouse gas nitrous oxide.
6. The use according to claim 1, wherein the tobacco planting soil is yellow soil.
7. The use according to claim 6, wherein the methyl p-coumarate and ammonium sulphate are applied together in the soil after being mixed into a solution, the application amount being 50-200 mg. N.kg -1 Soil.
8. Application of methyl p-coumarate in preparing tobacco seedling growth regulator is provided.
9. Application of methyl p-coumarate in preparing tobacco nitrogenous fertilizer products.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311075096.3A CN117084086A (en) | 2023-08-24 | 2023-08-24 | Application of p-coumarate methyl ester in promoting root growth and reducing nitrous oxide emission |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311075096.3A CN117084086A (en) | 2023-08-24 | 2023-08-24 | Application of p-coumarate methyl ester in promoting root growth and reducing nitrous oxide emission |
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| Publication Number | Publication Date |
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| CN117084086A true CN117084086A (en) | 2023-11-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311075096.3A Pending CN117084086A (en) | 2023-08-24 | 2023-08-24 | Application of p-coumarate methyl ester in promoting root growth and reducing nitrous oxide emission |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117084086A (en) |
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2023
- 2023-08-24 CN CN202311075096.3A patent/CN117084086A/en active Pending
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