CN117046388B - Device for high-pressure oxidation experiment of protein crystal - Google Patents
Device for high-pressure oxidation experiment of protein crystal Download PDFInfo
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- CN117046388B CN117046388B CN202311315504.8A CN202311315504A CN117046388B CN 117046388 B CN117046388 B CN 117046388B CN 202311315504 A CN202311315504 A CN 202311315504A CN 117046388 B CN117046388 B CN 117046388B
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- air inlet
- pressure cavity
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- base
- protein
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- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 44
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 44
- 239000013078 crystal Substances 0.000 title claims abstract description 38
- 238000002474 experimental method Methods 0.000 title claims abstract description 25
- 238000007254 oxidation reaction Methods 0.000 title claims abstract description 23
- 230000003647 oxidation Effects 0.000 title claims abstract description 21
- 238000007789 sealing Methods 0.000 claims abstract description 35
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 17
- 239000001301 oxygen Substances 0.000 claims abstract description 17
- 239000011521 glass Substances 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 238000011160 research Methods 0.000 description 7
- 238000000034 method Methods 0.000 description 5
- 229920001184 polypeptide Polymers 0.000 description 4
- 102000004196 processed proteins & peptides Human genes 0.000 description 4
- 108090000765 processed proteins & peptides Proteins 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 150000001371 alpha-amino acids Chemical class 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000006059 cover glass Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 238000002441 X-ray diffraction Methods 0.000 description 1
- 150000001413 amino acids Chemical group 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000012835 hanging drop method Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J3/00—Processes of utilising sub-atmospheric or super-atmospheric pressure to effect chemical or physical change of matter; Apparatus therefor
- B01J3/03—Pressure vessels, or vacuum vessels, having closure members or seals specially adapted therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J3/00—Processes of utilising sub-atmospheric or super-atmospheric pressure to effect chemical or physical change of matter; Apparatus therefor
- B01J3/04—Pressure vessels, e.g. autoclaves
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16J—PISTONS; CYLINDERS; SEALINGS
- F16J15/00—Sealings
- F16J15/02—Sealings between relatively-stationary surfaces
- F16J15/06—Sealings between relatively-stationary surfaces with solid packing compressed between sealing surfaces
- F16J15/10—Sealings between relatively-stationary surfaces with solid packing compressed between sealing surfaces with non-metallic packing
- F16J15/104—Sealings between relatively-stationary surfaces with solid packing compressed between sealing surfaces with non-metallic packing characterised by structure
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- General Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a device for high-pressure oxidation experiments of protein crystals, which relates to the technical field of biological experimental instruments, in particular to a device for high-pressure oxidation experiments of protein crystals, comprising a base, a sliding mechanism, a high-pressure cavity mechanism and a sealing air inlet mechanism, wherein a threaded opening is formed in the base, the threaded opening in the base is connected with the bottom end of a guide rail fixing frame, and a high-pressure cavity support is fixedly arranged on one side of the base; the sliding mechanism is arranged on the base through a guide rail fixing frame by a fixing screw, two guide rails which are distributed in parallel penetrate through the guide rail fixing frame, and a sliding block is arranged on each guide rail; the high-pressure cavity mechanism is arranged on the high-pressure cavity support, and an exhaust valve is arranged on one side of the high-pressure cavity mechanism; the sealed air inlet mechanism is fixed on the sliding block. The device for the high-pressure oxidation experiment of the protein crystal can enable the protein crystal to perform oxidation reaction in a high-pressure oxygen environment.
Description
Technical Field
The invention relates to the technical field of biological experiment instruments, in particular to a device for high-pressure oxidation experiments of protein crystals.
Background
Biological experiments refer to the process of purposefully observing and researching biological structures and vital activity phenomena which are not easy to observe under the general condition by using certain instruments, materials and medicines under specific environmental conditions through a scientific method; biology is a natural science based on experiments; through biological experiments, students can understand biological concepts and laws, truly learn basic biological knowledge, and are beneficial to inspiring the students to actively think, training scientific methods and culturing scientific quality of the students.
Biological laboratories have different emphasis and classification such as microbiological laboratories, cell biology laboratories, molecular biology laboratories, tissue culture laboratories, etc.; the usual equipment varies from biological laboratory to biological laboratory.
From the laboratory workflow, the method is divided into sample preservation, sample pretreatment, culture process, observation analysis and the like; different instruments are required in different working links.
Currently, research in life sciences has entered the "protein age"; the measurement of the complete three-dimensional structure of the protein, the complex and the assembly thereof is a scientific basis for researching the relationship between the molecular structure and the function in the life activity and revealing the physicochemical essence of the life phenomenon; x － ray diffraction of proteins and their complex crystals is one of the most dominant means to study the three-dimensional fine structure of biological macromolecules; study of physicochemical branching disciplines of protein crystal structure; the protein molecule is formed by polypeptide chains formed by condensing hundreds or more alpha - amino acids as monomers; a total of 20L-amino acids of the α - amino acids that can constitute the polypeptide chain in a protein; polypeptide chains of different amino acid sequences are formed by their different combinations and arrangements, and these polypeptide chains then further constitute millions of protein molecules by cross-linking.
However, the existing research equipment for protein crystals has large volume and high equipment use cost, so that the application range of the equipment is small and the equipment can be owned by a small number of researches, while the structure of the general high-pressure research equipment for protein crystals is complex, and the equipment needs enough operation experience base and cannot well meet the use requirements of people.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a device for high-pressure oxidation experiments of protein crystals, which solves the problems that the prior research equipment for protein crystals has larger volume and higher equipment use cost, so that the application scope of the equipment is smaller and the equipment can only be owned by a few researches, and the structure of the general high-pressure research equipment for protein crystals is complex and needs enough operation experience foundation.
In order to achieve the above purpose, the invention is realized by the following technical scheme: the device for the high-pressure oxidation experiment of the protein crystal comprises a base, a sliding mechanism, a high-pressure cavity mechanism and a sealing air inlet mechanism, wherein a threaded opening is formed in the base, the threaded opening in the base is connected with the bottom end of a guide rail fixing frame, and a high-pressure cavity support is fixedly arranged on one side of the base;
the sliding mechanism is arranged on the base through a guide rail fixing frame by a fixing screw, two guide rails which are distributed in parallel penetrate through the guide rail fixing frame, and a sliding block is arranged on each guide rail;
The high-pressure cavity mechanism comprises a circular cavity air chamber, an exhaust valve, an exhaust hole, an O-shaped rubber sealing ring, an exhaust hole and a threaded hole, wherein the O-shaped rubber sealing ring is arranged at the left side step of the circular cavity air chamber, the exhaust hole is positioned at the right side of the circular cavity air chamber, the center of the outer circular step of the high-pressure cavity mechanism is symmetrically provided with the threaded hole, the high-pressure cavity mechanism is arranged on a high-pressure cavity support, and the exhaust valve is arranged at the right side of the high-pressure cavity mechanism;
The sealed air inlet mechanism is fixed on the sliding block, a protein crystal plate is fixedly arranged on the right side of the sealed air inlet mechanism, an air inlet valve is fixedly arranged on the other side of the sealed air inlet mechanism, and a vacuum pump connecting valve is fixedly arranged on the left side of the air inlet valve through an oxygen pipe connector;
The sealed air inlet mechanism is a cylindrical step shaft and comprises an air inlet hole and a threaded interface, the left side of the air inlet hole is connected with the air inlet valve, and the right side of the air inlet hole penetrates through the sealed air inlet mechanism;
The central axis of the high-pressure cavity mechanism coincides with the central axis of the sealing air inlet mechanism, and the diameter of an inner circular arc at the left circular opening of the high-pressure cavity mechanism is consistent with the diameter of an outer circular arc at the right end of the sealing air inlet mechanism.
Optionally, the threaded interface is fixedly connected with the slider.
Optionally, the oxygen pipe connector is connected with an oxygen inlet pipe, and the vacuum pump connecting valve is connected with a vacuum pump pipe.
Optionally, the base is made of black organic glass.
Optionally, the protein crystal plate is made of transparent plastic material.
Optionally, the guide rails are symmetrically distributed about the central line of the sliding block, and the guide rails are parallel to the base.
The invention provides a device for a protein crystal high-pressure oxidation experiment, which has the following beneficial effects:
according to the device for the high-pressure oxidation experiment of the protein crystal, the high-pressure cavity mechanism can form a closed high-pressure inflation environment after being attached to the sealing air inlet mechanism, wherein the O-shaped rubber sealing ring can avoid the situation that gaps appear between the high-pressure cavity mechanism and the sealing air inlet mechanism after being attached, so that the tightness of the internal environment of the high-pressure cavity mechanism is maintained, and after the sealing air inlet mechanism is inserted into the high-pressure cavity mechanism, the operation of injecting air into the interior can be realized through an oxygen pipe connector, and the internal air pressure is sequentially improved;
The two parallel guide rails can provide limit guiding function for the sliding block, wherein the sliding block can realize the horizontal reciprocating movement operation of the sealing air inlet mechanism above the sliding block along the central axis direction of the guide rails, so that the plug-in connection between the sealing air inlet mechanism and the high-pressure cavity mechanism is changed; thereby the protein crystal can be subjected to oxidation reaction in high-pressure oxygen environment.
Drawings
FIG. 1 is a schematic diagram showing the structure of the device for high-pressure oxidation experiment of protein crystal in front view;
FIG. 2 is a schematic diagram of a high-pressure cavity mechanism and a sealed air inlet mechanism in a front full-section structure in the device for high-pressure oxidation experiment of protein crystal;
Fig. 3 is a schematic diagram of a front view full-section structure of the high-pressure cavity mechanism and the sealing air inlet mechanism in the device for high-pressure oxidation experiment of protein crystal.
In the figure: 1. a base; 101. a high pressure cavity support; 2. a sliding mechanism; 201. a guide rail fixing frame; 202. a guide rail; 203. a slide block; 204. a set screw; 3. a high pressure cavity mechanism; 301. a circular cavity air chamber; 302. an exhaust valve; 303. an exhaust hole; 304. an O-shaped rubber sealing ring; 305. a threaded hole; 4. sealing the air inlet mechanism; 401. a protein crystal plate; 402. an intake valve; 403. an oxygen pipe connector; 404. a vacuum pump connecting valve; 405. an air inlet hole; 406. a threaded interface.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments.
In the description of the present invention, unless otherwise indicated, the meaning of "a plurality" is two or more; the terms "upper," "lower," "left," "right," "inner," "outer," "front," "rear," "head," "tail," and the like are used as an orientation or positional relationship based on that shown in the drawings, merely to facilitate description of the invention and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "connected," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
Referring to fig. 1 to 3, the present invention provides a technical solution: the device for the high-pressure oxidation experiment of the protein crystal comprises a base 1, a sliding mechanism 2, a high-pressure cavity mechanism 3 and a sealing air inlet mechanism 4, wherein a threaded opening is formed in the base 1, the threaded opening in the base 1 is connected with the bottom end of a guide rail fixing frame 201, and a high-pressure cavity support 101 is fixedly arranged on one side of the base 1;
the sliding mechanism 2 is arranged on the base 1 through a fixed screw 204 by a guide rail fixing frame 201, two guide rails 202 which are distributed in parallel penetrate through the guide rail fixing frame 201, and a sliding block 203 is arranged on the guide rail 202;
The high-pressure cavity mechanism 3 comprises a circular cavity air chamber 301, an exhaust valve 302, an exhaust hole 303, an O-shaped rubber sealing ring 304, the exhaust hole 303 and a threaded hole 305, wherein the O-shaped rubber sealing ring 304 is arranged at the left side step of the circular cavity air chamber 301, the exhaust hole 303 is positioned at the right side of the circular cavity air chamber 301, the threaded hole 305 is symmetrically arranged at the center of the outer circular step of the high-pressure cavity mechanism 3, the high-pressure cavity mechanism 3 is arranged on the high-pressure cavity support 101, the exhaust valve 302 is arranged at the right side of the high-pressure cavity mechanism 3, the high-pressure cavity mechanism 3 can form a closed high-pressure inflation environment after being attached to the sealing air inlet mechanism 4, and the O-shaped rubber sealing ring 304 can avoid the situation that gaps appear between the O-shaped rubber sealing ring 304 after being attached, so that the tightness of the internal environment of the high-pressure cavity mechanism 3 is maintained;
The sealed air inlet mechanism 4 is fixed on the sliding block 203, a protein crystal plate 401 is fixedly arranged on the right side of the sealed air inlet mechanism 4, an air inlet valve 402 is fixedly arranged on the other side of the sealed air inlet mechanism 4, and a vacuum pump connecting valve 404 is fixedly arranged on the left side of the air inlet valve 402 through an oxygen pipe connector 403;
The sealed air inlet mechanism 4 is a cylindrical stepped shaft and comprises an air inlet hole 405 and a threaded interface 406, the left side of the air inlet hole 405 is connected with the air inlet valve 402, and the right side of the air inlet hole 405 penetrates through the sealed air inlet mechanism 4;
The central axis of the high-pressure cavity mechanism 3 coincides with the central axis of the sealing air inlet mechanism 4, and the diameter of an inner circular arc at the left circular opening of the high-pressure cavity mechanism 3 coincides with the diameter of an outer circular arc at the right end of the sealing air inlet mechanism 4.
In this embodiment, as shown in fig. 1, the threaded interface 406 is fixedly connected to the slider 203.
In this embodiment, as shown in fig. 1, an oxygen pipe connector 403 is connected to an oxygen inlet pipe, and a vacuum pump connecting valve 404 is connected to a vacuum pump pipe; after the sealed air inlet mechanism 4 is inserted into the high-pressure cavity mechanism 3, the operation of injecting air into the interior can be realized through the oxygen pipe connector 403, and the internal air pressure is sequentially improved.
In this embodiment, as shown in fig. 1, the base 1 is made of black organic glass.
In this embodiment, as shown in FIG. 1, the protein crystal plate 401 is made of transparent plastic.
In this embodiment, as shown in fig. 1, the guide rails 202 are symmetrically distributed about the center line of the slider 203, and the guide rails 202 are parallel to the base 1; the two parallel guide rails 202 can provide a limit guiding function for the sliding block 203, wherein the sliding block 203 can realize the horizontal reciprocating movement operation of the sealing air inlet mechanism 4 above the sliding block along the central axis direction of the guide rails 202, so as to change the insertion connection between the sealing air inlet mechanism 4 and the high-pressure cavity mechanism 3.
In summary, when the device for high-pressure oxidation experiments of protein crystals is used, firstly, an air inlet pipe is connected with an oxygen pipe connector 403, a vacuum pump is connected with a vacuum pump connecting valve 404, a mixed solution is added into a hole of a protein crystal plate 401, the protein solution and the mixed solution are dripped on a cover glass by using a hanging drop method, the cover glass is placed on the hole of the protein crystal plate 401, a sealing air inlet mechanism 4 is pushed to be close to a high-pressure cavity mechanism 3, the sealing air inlet mechanism 4 is in threaded connection with the high-pressure cavity mechanism 3 for sealing by manually rotating the high-pressure cavity mechanism 3, an exhaust valve 302 is closed, an air inlet valve 402 is opened, the vacuum pump connecting valve 404 is opened, air in a circular cavity air chamber 301 is evacuated, the vacuum pump connecting valve 404 is closed, high-pressure oxygen is opened to inject high-pressure oxygen into the high-pressure cavity mechanism 3, and protein crystallization is waited for.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
Claims (6)
1. The utility model provides a device for protein crystal high pressure oxidation experiment, includes base (1), slide mechanism (2), high pressure cavity mechanism (3) and sealed mechanism (4) that admits air, its characterized in that: the high-pressure cavity support (101) is fixedly arranged on one side of the base (1);
the sliding mechanism (2) is arranged on the base (1) through a guide rail fixing frame (201) by a fixing screw (204), two guide rails (202) which are distributed in parallel are penetrated in the guide rail fixing frame (201), and a sliding block (203) is arranged on the guide rails (202);
The high-pressure cavity mechanism (3) comprises a circular cavity air chamber (301), an exhaust valve (302), an exhaust hole (303), an O-shaped rubber sealing ring (304), an exhaust hole (303) and a threaded hole (305), wherein the O-shaped rubber sealing ring (304) is arranged at the left side step of the circular cavity air chamber (301), the exhaust hole (303) is positioned at the right side of the circular cavity air chamber (301), the threaded hole (305) is symmetrically formed in the center of the outer circular step of the high-pressure cavity mechanism (3), the high-pressure cavity mechanism (3) is arranged on the high-pressure cavity support (101), and the exhaust valve (302) is arranged at the right side of the high-pressure cavity mechanism (3);
The sealing air inlet mechanism (4) is fixed on the sliding block (203), a protein crystal plate (401) is fixedly arranged on the right side of the sealing air inlet mechanism (4), an air inlet valve (402) is fixedly arranged on the other side of the sealing air inlet mechanism (4), and a vacuum pump connecting valve (404) is fixedly arranged on the left side of the air inlet valve (402) through an oxygen pipe connector (403);
The sealed air inlet mechanism (4) is a cylindrical step shaft and comprises an air inlet hole (405) and a threaded interface (406), the left side of the air inlet hole (405) is connected with the air inlet valve (402), and the right side of the air inlet hole (405) penetrates through the sealed air inlet mechanism (4);
The central axis of the high-pressure cavity mechanism (3) coincides with the central axis of the sealing air inlet mechanism (4), and the diameter of an inner circular arc at the left circular opening of the high-pressure cavity mechanism (3) coincides with the diameter of an outer circular arc at the right end of the sealing air inlet mechanism (4).
2. The apparatus for high-pressure oxidation experiments of protein crystals according to claim 1, wherein: the threaded interface (406) is fixedly connected with the sliding block (203).
3. The apparatus for high-pressure oxidation experiments of protein crystals according to claim 1, wherein: the oxygen pipe connector (403) is connected with an oxygen inlet pipe, and the vacuum pump connecting valve (404) is connected with a vacuum pump pipe.
4. The apparatus for high-pressure oxidation experiments of protein crystals according to claim 1, wherein: the base (1) is made of black organic glass.
5. The apparatus for high-pressure oxidation experiments of protein crystals according to claim 1, wherein: the protein crystal plate (401) is made of transparent plastic material.
6. The apparatus for high-pressure oxidation experiments of protein crystals according to claim 1, wherein: the guide rails (202) are symmetrically distributed about the central line of the sliding block (203), and the guide rails (202) are parallel to the base (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311315504.8A CN117046388B (en) | 2023-10-12 | 2023-10-12 | Device for high-pressure oxidation experiment of protein crystal |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311315504.8A CN117046388B (en) | 2023-10-12 | 2023-10-12 | Device for high-pressure oxidation experiment of protein crystal |
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| Publication Number | Publication Date |
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| CN117046388A CN117046388A (en) | 2023-11-14 |
| CN117046388B true CN117046388B (en) | 2024-05-24 |
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| CN202311315504.8A Active CN117046388B (en) | 2023-10-12 | 2023-10-12 | Device for high-pressure oxidation experiment of protein crystal |
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| CN117046388A (en) | 2023-11-14 |
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