CN219417114U

CN219417114U - Quartz crystal microbalance testing instrument

Info

Publication number: CN219417114U
Application number: CN202320387092.8U
Authority: CN
Inventors: 孟卓君; 朱滔滔; 张鹏飞; 张开翔
Original assignee: Wenzhou Research Institute Of Guoke Wenzhou Institute Of Biomaterials And Engineering
Current assignee: Wenzhou Research Institute Of Guoke Wenzhou Institute Of Biomaterials And Engineering
Priority date: 2023-02-24
Filing date: 2023-02-24
Publication date: 2023-07-25
Anticipated expiration: 2033-02-24

Abstract

The utility model discloses a quartz crystal microbalance testing instrument, which comprises a main body, wherein a pre-sampling module and a testing module are arranged on the main body, the pre-sampling module comprises a first sample cell and a first cover body arranged at the top of the first sample cell, and the testing module comprises a second sample cell, a quartz crystal microbalance testing sensor arranged at the inner bottom of the second sample cell and a second cover body arranged at the top of the second sample cell; the first cover body and the second cover body are provided with communication holes for the insertion of pipelines for communicating the first sample tank and the second sample tank, the first cover body is also provided with sample injection holes, and the two cover bodies are also provided with sample outlet holes. The utility model is additionally provided with the pre-sampling sample pool, so that the sample solution can be uniformly mixed before detection.

Description

Quartz crystal microbalance testing instrument

Technical Field

The utility model relates to the technical field of biological detection, in particular to a quartz crystal microbalance testing instrument.

Background

The quartz crystal microbalance (Quartz Crystal Microbalance, QCM) is a high-sensitivity piezoelectric sensor capable of detecting in real time without fluorescent calibration, and can be used for monitoring surface analysis of biological materials, interaction of proteins, adsorption analysis of membrane surfaces, hybridization of DNA (deoxyribonucleic acid) on the surfaces of the biological membranes, degradation of enzymes, research of targeted drugs, research of characteristics such as polymer swelling and structure change, biocompatibility of polymer materials and the like in real time. The method has the advantages of high resolution, high sensitivity, good specificity, real-time online monitoring and the like, and is widely applied to the fields of biotechnology, medical equipment, energy cleaning, food pharmacy and the like.

The QCM mainly comprises a quartz crystal sensor, a signal collection part, a signal detection part, a data processing part and the like. Currently, most commonly used QCM instruments are single-channel and four-channel, for example, patent specification with publication number CN213239118U discloses a QCM single-channel detection cell, which comprises a housing, wherein the housing is provided with a liquid inlet and a liquid outlet; the shell is internally provided with a microfluidic channel and a detection cavity, one end of the microfluidic channel is communicated with the liquid inlet, and the other end of the microfluidic channel is communicated with the inlet of the detection cavity; the outlet of the detection cavity is communicated with the liquid outlet; the detection cavity is internally provided with a sensing chip for detection, the shell is also provided with a metal conductor for transmitting frequency signals, one end of the conductor is connected with the sensing chip, and the other end of the conductor extends out of the shell.

The single-channel detection cell of the scheme is not provided with the pre-sampling sample cell, and the sample mixed solution is required to be uniformly mixed in other containers in advance before detection, if the sample and the buffer solution are directly pumped in, the problem of non-uniform mixing can be caused, and the detection effect is affected.

Disclosure of Invention

The utility model aims to provide a quartz crystal microbalance testing instrument, which is additionally provided with a pre-sampling sample tank, so that the temperature control and the uniform mixing of a sample solution can be realized before the detection.

The quartz crystal microbalance testing instrument comprises a main body, and is characterized in that a pre-sampling module and a testing module are arranged on the main body, the pre-sampling module comprises a first sample cell and a first cover body arranged at the top of the first sample cell, and the testing module comprises a second sample cell, a quartz crystal microbalance testing sensor arranged at the inner bottom of the second sample cell and a second cover body arranged at the top of the second sample cell; the first cover body and the second cover body are provided with communication holes for the insertion of pipelines for communicating the first sample tank and the second sample tank, the first cover body is also provided with sample injection holes, and the two cover bodies are also provided with sample outlet holes.

Before detection, a communicating pipe is inserted into the two communicating holes to realize the communication between the first sample cell and the second sample cell, a sample inlet pipe is inserted into the sample inlet hole, the other end of the sample inlet pipe is used for extracting samples and buffer solution, a sample outlet pipe is inserted into the sample outlet hole, the other end of the sample outlet pipe is connected with the inlet end of a peristaltic pump, and the transport of sample solution is realized through the operation of the peristaltic pump; the sample solution firstly enters the pre-sampling module for uniform mixing, and then enters the testing module for detection after reaching the target temperature.

When the quartz crystal microbalance chip needs to be regenerated or cleaned, the buffer solution or the chip cleaning liquid can be directly pumped into the test module through the sample injection pipe to regenerate or clean the chip.

Preferably, a heating and refrigerating sheet is arranged at the bottom of the first sample tank of the pre-sampling module, and the temperature range can be controlled to be 4-95 ℃.

Under the action of the peristaltic pump, a sample firstly enters a sample tank of the pre-sampling module through a hose and is preheated, and after the temperature reaches the target temperature, the sample is rapidly pumped into the testing module through the peristaltic pump.

Preferably, the main body comprises two detachable parts, and each part is provided with a pre-sampling module and a testing module. The two parts can be used for simultaneously carrying out a test experiment and a control experiment, and can be independently tested after being disassembled, so that the device is small and convenient in size and can be used together with a microscope.

Further preferably, the two parts of the body are detachably connected by means of a socket, eye-splice arrangement located on opposite side walls of the two parts.

Preferably, the second cover body adopts transparent design, makes things convenient for experimental observation and uses with the microscope.

Preferably, the pre-sampling module and the testing module are both provided with signal lamps for displaying whether the module starts to operate, and when the connection is normal, the green light is turned on.

The utility model has the beneficial effects that:

(1) The utility model can simultaneously carry out two groups of experiments, namely a test experiment and a control experiment.

(2) The pre-sampling module can regulate and control the experimental temperature, can carry out specific precise experiments with requirements on temperature, and can also realize the uniform mixing of sample solutions.

(3) The detachable design can decompose the instrument into two independent test modules, and the instrument size is small and exquisite after the dismantlement, and convenient and microscope combination is used, and the instrument is light and handy portable simultaneously, is fit for removing the test scene.

Drawings

FIG. 1 is a schematic diagram of the structure of the present utility model;

FIG. 2 is a schematic view of the structure of the utility model (uncapping);

in the figure: 1. a pre-sampling module; 11. a first sample cell; 12. a first cover; 121. a communication hole; 122. a sample inlet; 2. a test module; 21. a second sample cell; 22. a second cover; 221. a communication hole; 222. a sample outlet hole; 23. a quartz crystal microbalance test sensor; 3. a signal lamp.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

As shown in fig. 1 and 2, a quartz crystal microbalance testing instrument comprises a main body 100, wherein a pre-sampling module 1 and a testing module 2 are arranged on the main body 100.

The pre-sampling module 1 comprises a first sample cell 11 and a first cover 12 arranged on the top of the first sample cell 11.

The test module 2 comprises a second sample cell 21, a second cover 22 arranged at the top of the second sample cell 21 and a quartz crystal microbalance test sensor 23 arranged at the bottom in the second sample cell 21.

The first cover 12 and the second cover 22 are provided with communication holes 121 and 221, and the first sample cell 11 and the second sample cell 12 can be communicated through the communication holes, so that the first sample cell 11 and the second sample cell 12 form a communicated closed space.

In this embodiment, the second cover 22 is transparent, so that experimental observation and combination with a microscope are facilitated.

The first cover 12 is also provided with a sample inlet 122, and the first sample pool 11 and the sample container can be communicated by inserting the sample inlet; the second cover 22 is also provided with a sample outlet 222 which is connected with the inlet end of the peristaltic pump after being inserted into the sample outlet pipe; and through the operation of a peristaltic pump, the sample is conveyed through the pre-sampling module 1 and the testing module 2.

In this embodiment, the pre-sampling module 1 further includes a heating and cooling plate disposed at the bottom of the first sample cell 11, where the temperature range can be controlled to be 4-95 ℃.

Under the action of the peristaltic pump, a sample firstly enters a sample cell of the pre-sampling module 1 from a sampling pipe and is preheated, and after the temperature reaches the target temperature, the sample is rapidly pumped into the testing module 2 by the peristaltic pump.

In this embodiment, the main body 100 includes two detachable parts, and each part is provided with a pre-sampling module 1 and a testing module 2; the two parts can be used for simultaneously carrying out a test experiment and a control experiment, and can be independently tested after being disassembled, so that the device is small and convenient in size and can be used together with a microscope. The two parts of the main body 100 may be detachably connected by means of a socket and a buckle structure provided on opposite side walls of the two parts.

In this embodiment, the sample injection module 1 and the test module 2 are both provided with a signal lamp 3 for displaying whether the module starts to operate, and when the connection is normal, the green lamp is turned on.

Although the present utility model has been described with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described, or equivalents may be substituted for elements thereof, and any modifications, equivalents, improvements and changes may be made without departing from the spirit and principles of the present utility model.

Claims

1. The quartz crystal microbalance testing instrument comprises a main body, and is characterized in that a pre-sampling module and a testing module are arranged on the main body, the pre-sampling module comprises a first sample cell and a first cover body arranged at the top of the first sample cell, and the testing module comprises a second sample cell, a quartz crystal microbalance testing sensor arranged at the inner bottom of the second sample cell and a second cover body arranged at the top of the second sample cell; the first cover body and the second cover body are provided with communication holes for the insertion of pipelines for communicating the first sample tank and the second sample tank, the first cover body is also provided with sample injection holes, and the two cover bodies are also provided with sample outlet holes.

2. The quartz crystal microbalance testing instrument according to claim 1, wherein a heating and cooling plate is arranged at the bottom of the first sample cell of the pre-sampling module, and the controllable temperature range is 4-95 ℃.

3. The quartz crystal microbalance testing instrument according to claim 1, wherein the body comprises two detachable parts, and each part is provided with a pre-sampling module and a testing module.

4. A quartz crystal microbalance testing instrument according to claim 3, wherein the two parts of the body are detachably connected by means of a socket, eye-splice arrangement located on opposite side walls of the two parts.

5. The quartz crystal microbalance testing instrument of claim 1, wherein the second cover is of transparent design.

6. The quartz crystal microbalance testing instrument according to claim 1, wherein the pre-sampling module and the testing module are each provided with a signal lamp indicating whether the module is started to operate.